run_metadata: 33877
This data as json
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| 33877 | SRR30831722 | SRX26231007 | SRS22772579 | SRP535562 | PRJNA1166865 | Transcriptome data of zebrafish with glula knockdown and glulb knockdown | PRJNA1166865 | Other | To investigate global changes in gene expression following the knockdown of glula or glulb in zebrafish we employed RNA sequencing to analyze the whole genome transcriptomes of glula or glulb knockdown zebrafish. Three dpf wild type zebrafish and zebrafish injected with glula MO and glulb MO were prepared for analysis with three independent replicates performed for each treatment. The total RNA extraction was performed using TRIzol reagent Invitrogen United States according to the manufacturer's protocol. Subsequently the quality and quantity of total RNAs were assessed using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit Agilent CA United States ensuring that the RIN values exceeded 7.0. The RNA samples were subsequently submitted to GENEWIZ Science Suzhou China for deep sequencing using an Illumina Hiseq2500 platform. | Mo glulb | Mo glulb3 | strain:Zebrafish|isolate:glulb knock low and repeat3|age:3 days|dev stage:embryo|collection date:2020|geo loc name:China: Jiangsu|sex:pooled male and female|tissue:embryo|cell type:embryo|genotype:knockdown glulb|BioSampleModel:Model organism or animal | RNA seq of zebrafish | B3 | B3 | Three dpf zebrafish injected with glulb MO embryo | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP535562 | glulb-mo-3_combined_R1.fastq.gzip glulb-mo-3_combined_R2.fastq.gzip | fastq fastq | 6765297900.0 | 22550993.0 | glulb mo 3 combined R1.fastq.gzip | 0:150 1:150 | A:1696049453;C:1697070390;G:1692526372;T:1679586337;N:65348 | 150 | 150 | 1696049453 | 1697070390 | 1692526372 | 1679586337 | 65348 | SRX26231007 | SRS22772579 | SRA1981714 | Nantong University|School of Life Sciences | Nantong University | B | B | biological fallback assumption | illumina | hiseq_era | full_length | poly_a | unknown | bulk | unknown | unknown | China | 2024-09-29 | Larval | Larval | Embryo Imprecise | All anatomical structures |