run_metadata: 33387
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 33387 | SRR30184293 | SRX25650003 | SRS22293072 | SRP525334 | PRJNA1145997 | Regulation of optic nerve regeneration by srebf2 | PRJNA1145997 | Other | Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model. | heatshock srebf2MO 3 | hs smo1 F | strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock srebf2MO 3|BioSampleModel:Model organism or animal | LCM seq of Danio rerio:retinal ganglion cell layer | L27 | L27 | LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene | RNA-Seq | TRANSCRIPTOMIC | cDNA_oligo_dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP525334 | L27_S115_L004_R2_001.fastq.gz L27_S115_L004_R1_001.fastq.gz | fastq fastq | 22610315690.0 | 74868595.0 | L27 S115 L004 R1 001.fastq.gz | 0:151 1:151 | A:6205491974;C:5075129196;G:4965212019;T:6361870398;N:2612103 | 151 | 151 | 6205491974 | 5075129196 | 4965212019 | 6361870398 | 2612103 | SRX25650003 | SRS22293072 | SRA1943640 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.78769 | 0.78807 | 0.09282 | 0.09299 | 0.76619 | 0.7651 | 0.54884 | 0.5509 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | United States | 2024-08-09 | Adult | Adult | Eye | Sensory System |