run_metadata: 33311

This data as json

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33311	SRR29927545	SRX25421505	SRS22081661	SRP521592	PRJNA1139127	Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration	PRJNA1139127	Other	Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration.				ON 3d rep3	MCWR 2019 000157		strain:wild type\|dev stage:adult\|collection date:2019 10 18\|geo loc name:USA: Wisconsin Milwaukee\|sex:female\|tissue:optic nerve\|treatment:ON 3d rep3\|BioSampleModel:Model organism or animal									RNA Seq of Danio rerio: 3 xxx post injury optic nerve	ON 3d rep3	ON 3d rep3	SMART Seqv4			RNA-Seq	TRANSCRIPTOMIC	Oligo-dT	PAIRED	ILLUMINA	Illumina HiSeq 2000		SRP521592			MCWR_2019_000157_S28_L001_R1_001.fastq.gz MCWR_2019_000157_S28_L001_R2_001.fastq.gz MCWR_2019_000157_S28_L002_R1_001.fastq.gz MCWR_2019_000157_S28_L002_R2_001.fastq.gz	fastq fastq fastq fastq	12637756180.0	41976869.0	MCWR 2019 000157 S28 L001 R1 001.fastq.gz	0:150.53 1:150.53	A:3303913534;C:3007130754;G:3036624621;T:3289934437;N:152834	150	150			3303913534	3007130754	3036624621	3289934437	152834	SRX25421505	SRS22081661	SRA1930690	Medical College of Wisconsin\|Cell Biology, Neurobiology, and Anatomy	Medical College of Wisconsin	2	0.9516	0.95161	0.06268	0.06324	0.7245	0.72539	0.50499	0.5082	151	151	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	sc	single_cell_plate	smartseq		United States	2024-07-23	Adult	Adult	Brain	Nervous System