run_metadata: 32875
This data as json
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| 32875 | SRR29488935 | SRX24999618 | SRS21700578 | SRP515253 | PRJNA1126644 | Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity | GSE270442 | Transcriptome Analysis | Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. | ALDH3A1KO5 | GSM8343030 | source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO|geo loc name:missing|collection date:missing | ALDH3A1KO5 | Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. | larvae | Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library. | tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO | GSM8343030 | GSM8343030: ALDH3A1KO5; Danio rerio; RNA Seq | GSM8343030 r1 | GSM8343030 | 1 | Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | SRP515253 | ALDH3A1KO5_1.fq.gz ALDH3A1KO5_2.fq.gz | fastq fastq | 4811331000.0 | 24056655.0 | GSM8343030 r1 | 0:100 1:100 | A:1314568654;C:1091419677;G:1111494171;T:1293848498;N:0 | 100 | 100 | 1314568654 | 1091419677 | 1111494171 | 1293848498 | 0 | SRX24999618 | SRS21700578 | SRA1904686 | ECAS | ECAS | B | B | biological fallback assumption | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | Unknown | 2024-06-21 | Larval | Larval | Cell Line | Cell Line |