run_metadata: 31902

This data as json

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31902	SRR28763293	SRX24328715	SRS21090019	SRP503258	PRJNA1102381	Acidification alters anxiety like behaviour and brain gene expression in zebrafish	PRJNA1102381	Other	CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain acidification caused differential expression of genes involved in cytoskeletal organization cellular transport immunity and the visual neural system. In the gills an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.					EB19		replicate name:10\|breed:AB\|age:4 months\|dev stage:adult\|collection date:2021 11 14\|geo loc name:Hong Kong\|sex:not determined\|tissue:brain\|BioSampleModel:Model organism or animal									RNA Seq of 4mpf Danio rerio brain	EB19	EB19	Total RNA was extracted using the RNeasy Micro Kit Qiagen.			RNA-Seq	TRANSCRIPTOMIC	PolyA	PAIRED	ILLUMINA	Illumina NovaSeq 6000		SRP503258			EB19_1.fastq.gz EB19_2.fastq.gz	fastq fastq	9436386526.0	31246313.0	EB19 1.fastq.gz	0:151 1:151	A:2716315062;C:1976294752;G:2147671436;T:2595980360;N:124916	151	151			2716315062	1976294752	2147671436	2595980360	124916	SRX24328715	SRS21090019	SRA1849282	The University of Hong Kong\|School of Biological Sciences	The University of Hong Kong	2	0.91409	0.91345	0.24896	0.24788	0.78056	0.78279	0.72332	0.73168	151	151	B	B	biological fallback assumption	illumina	novaseq_era	unknown	poly_a	unknown	bulk	unknown	unknown		China	2024-04-22	Adult	Adult	Brain	Nervous System