run_metadata: 2320
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2320 | ERR1289946 | ERX1361552 | ERS954842 | ERP012920 | PRJEB11523 | Zebrafish eye populations transcriptomics | Zebrafish_eye_populations_transcriptomics-sc-3967 | Transcriptome Analysis | To characterise the transcriptome of the stem cells and the proliferative progenitors in the retina of 5 dpf zebrafish Danio rerio eyes were prepared as follows: 20 30 eyes per condition were collected dissociated and FACS sorted. The samples were collected to RLT buffer and RNA extraction was immediately performed. cDNA and Nextera XT libraries were generated and subsequent sequencing was carried out using the Illumina HiSeq 2500 platform. | ArrayExpress:E ERAD 441 | SAMEA3647693 | SC | ArrayExpress OrganismPart:eye cell populations|ArrayExpress Sex:mixed|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2016 02 23T15:26:28Z|ENA LAST UPDATE:2018 03 09T09:23:00Z|External Id:SAMEA3647693|INSDC center name:SC|INSDC first public:2016 02 23T15:26:28Z|INSDC last update:2018 03 09T09:23:00Z|INSDC status:public|Submitter Id:WT ctrl sc 2454964|common name:zebrafish|sample description:eye cell populations from zebrafish embryo|sample name:WT ctrl sc 2454964|scientific name:Danio rerio | Illumina HiSeq 2500 paired end sequencing | SC EXP 18222 2#11 | 15249492 | Illumina sequencing of library 15249492 constructed from sample accession ERS954842 for study accession ERP012920. This is part of an Illumina multiplexed sequencing run 18222 2. This submission includes reads tagged with the sequence TAGGCATGTATCCTCT. | Nextera dual index qPCR only | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP012920 | Illumina HiSeq 2500 paired end sequencing | ENA FIRST PUBLIC:2016 02 23|ENA LAST UPDATE:2018 11 16 | 18222_2#11.cram | cram | 2748114600.0 | 13740573.0 | SC RUN 18222 2#11 | 0:100 1:100 | A:747132237;C:630918171;G:613637334;T:756413030;N:13828 | 100 | 100 | 747132237 | 630918171 | 613637334 | 756413030 | 13828 | ERX1361552 | ERS954842 | ERA565862 | European Nucleotide Archive | Wellcome Sanger Institute | 2 | 0.91201 | 0.911 | 0.09071 | 0.09156 | 0.88994 | 0.88988 | 0.47118 | 0.49948 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | nextera | bulk | unknown | unknown | United Kingdom | 2016-02-23 | Undetermined | Embryo | Eye | Sensory System |