run_metadata: 15074
This data as json
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| 15074 | ERR12476438 | ERX11852259 | ERS17743534 | ERP156655 | PRJEB71869 | Effects of paternal starvation in the offspring development of zebrafish | 33b9d14c-4211-4249-aede-f0f021d197e6 | Other | Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. | ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15 | 24 hpf embryo collected at 1118 from a cross with a fed father | 1118 Fed | 1118F | organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden | NextSeq 500 sequencing | ena EXPERIMENT TAB 15 01 2024 21:42:36:173 276973 | 1118F | 1 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | NextSeq 500 | ERP156655 | NextSeq 500 sequencing | ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 | 1118F_S9_L003_R1_001.fastq.gz | fastq | 98443412.0 | 1309747.0 | ena RUN TAB 15 01 2024 21:42:36:173 276974 | 0:75.16 | A:35171021;C:18991511;G:20974950;T:23280285;N:25645 | 75 | 35171021 | 18991511 | 20974950 | 23280285 | 25645 | ERX11852259 | ERS17743534 | ERA27788968 | University of Birmingham|European Nucleotide Archive | University of Birmingham | B | usable mapping rate | illumina | nextseq | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | United Kingdom | 2024-01-15 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures |