run_metadata: 11711
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 11711 | ERR11282347 | ERX10689990 | ERS15395535 | ERP146895 | PRJEB61813 | RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month | E-MTAB-12901 | Other | The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. | ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 | Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection | Sample 8 | SAMEA113400389 | VANDERBILT UNIVERSITY | ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400389|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 8|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 8|scientific name:Danio rerio|sex:female|strain:AB | Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month | E MTAB 12901:Sample 8 p | Sample 8 p | RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month | brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection | Experimental Factor: genotype:wild type genotype | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | ERP146895 | Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month | ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 | 8680-JP-0008_S1_L005_R2_001.fastq.gz 8680-JP-0008_S1_L005_R1_001.fastq.gz | fastq fastq | 21919844332.0 | 72582266.0 | E MTAB 12901:8680 JP 0008 S1 L005 R | 0:151 1:151 | A:5996325335;C:4770833694;G:5244802952;T:5876120950;N:31761401 | 151 | 151 | 5996325335 | 4770833694 | 5244802952 | 5876120950 | 31761401 | ERX10689990 | ERS15395535 | ERA23235100 | VANDERBILT UNIVERSITY|European Nucleotide Archive | VANDERBILT UNIVERSITY|European Nucleotide Archive | 2 | 0.92572 | 0.92425 | 0.13427 | 0.13101 | 0.70086 | 0.70266 | 0.50868 | 0.50381 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | United States | 2023-07-14 | Adult | Adult | Brain | Nervous System |