run_metadata: 10335
This data as json
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| 10335 | ERR7384915 | ERX6953419 | ERS8736620 | ERP133301 | PRJEB48872 | Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon | E-MTAB-11163 | Transcriptome Analysis | Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration. | ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31 | Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina | 1dpl 3 | SAMEA11086956 | Izmir Biomedicine and Genome Center | ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086956|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:1dpl 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|injury:stab lesion|organism part:telencephalon|sample name:E MTAB 11163:1dpl 3|sex:male|strain:AB | NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon | E MTAB 11163:1dpl 3 p | 1dpl 3 p | Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon | Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina | Experimental Factor: injury:stab lesion|Experimental Factor: time:1 | RNA-Seq | TRANSCRIPTOMIC | other | PAIRED | ILLUMINA | NextSeq 500 | ERP133301 | NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon | ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31 | 1dpl-3_1.fastq.gz 1dpl-3_2.fastq.gz | fastq fastq | 9373333280.0 | 58583333.0 | E MTAB 11163:1dpl 3 | 0:80 1:80 | A:2671556387;C:2008711931;G:2062635156;T:2629594613;N:835193 | 80 | 80 | 2671556387 | 2008711931 | 2062635156 | 2629594613 | 835193 | ERX6953419 | ERS8736620 | ERA7396457 | Izmir Biomedicine and Genome Center|European Nucleotide Archive | Izmir Biomedicine and Genome Center|European Nucleotide Archive | 2 | 0.93438 | 0.93842 | 0.21075 | 0.20463 | 0.71459 | 0.71246 | 0.50094 | 0.50105 | 80 | 80 | B | B | biological fallback assumption | illumina | nextseq | full_length | random_priming | trueseq | bulk | unknown | unknown | Turkey | 2022-01-31 | Adult | Adult | Brain | Nervous System |