rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 50723,SRR8270602,SRX5087288,SRS4099391,SRP171998,PRJNA507898,Single cell RNA Seq reveals Fgf signaling dynamics during sensory hair cell regeneration,GSE123241,Transcriptome Analysis,Loss of sensory hair cells leads to deafness and balance deficiencies. In contrast to mammalian hair cells zebrafish ear and lateral line hair cells regenerate from poorly characterized proliferating support cells. Equally ill defined is the gene regulatory network underlying the progression of support cells to cycling hair cell progenitors and differentiated hair cells. We used single cell RNA Sequencing scRNA Seq of lateral line sensory organs and uncovered five different support cell types including quiescent and activated stem cells. In silico ordering of support cells along a developmental trajectory identified cells that self renew and new groups of genes required for hair cell differentiation. scRNA Seq analyses of fgf3 mutants in which hair cell regeneration is increased demonstrates that Fgf and Notch signaling inhibit proliferation of support cells in parallel by inhibiting Wnt signaling. Our scRNA Seq analyses set the foundation for mechanistic studies of sensory organ regeneration and is crucial for identifying factors to trigger hair cell production in mammals. Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 3 different samples: wild type fgf3 mutants and fgf3 siblings.,,pubmed:30681411;pubmed:31488837,,fgf3 mutant,GSM3498554,,source name:neuromast|age:5dpf|tissue:neuromast|genotype:fgf3 / |strain:fgf3^t26212; ETkrt4:EGFPSqGw57A; Tgpou4f3:GAP GFPs356t screened for mutants,fgf3 mutant,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|genotype:fgf3 / |strain:fgf3^t26212;ETkrt4:EGFPSqGw57A;Tgpou4f3:GAP GFPs356t screened for mutants,GSM3498554,GSM3498554: fgf3 mutant; Danio rerio; RNA Seq,GSM3498554,,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,GEO Accession:GSM3498554,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP171998,,,possorted_genome_bam_fgf3_mut.bam,10X Genomics bam file,16974126576.0,171455824.0,GSM3498554 r1,0:99,A:5250835858;C:3266700009;G:3497115040;T:4840312852;N:119162817,99,,,,5250835858,3266700009,3497115040,4840312852,119162817,SRX5087288,SRS4099391,SRA817952,GEO,Stowers Institute,1,0.89941,,0.21879,,0.80805,,0.48702,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2018-12-02,Larval,Larval,Lateral Line,Sensory System 50724,SRR8270601,SRX5087287,SRS4099390,SRP171998,PRJNA507898,Single cell RNA Seq reveals Fgf signaling dynamics during sensory hair cell regeneration,GSE123241,Transcriptome Analysis,Loss of sensory hair cells leads to deafness and balance deficiencies. In contrast to mammalian hair cells zebrafish ear and lateral line hair cells regenerate from poorly characterized proliferating support cells. Equally ill defined is the gene regulatory network underlying the progression of support cells to cycling hair cell progenitors and differentiated hair cells. We used single cell RNA Sequencing scRNA Seq of lateral line sensory organs and uncovered five different support cell types including quiescent and activated stem cells. In silico ordering of support cells along a developmental trajectory identified cells that self renew and new groups of genes required for hair cell differentiation. scRNA Seq analyses of fgf3 mutants in which hair cell regeneration is increased demonstrates that Fgf and Notch signaling inhibit proliferation of support cells in parallel by inhibiting Wnt signaling. Our scRNA Seq analyses set the foundation for mechanistic studies of sensory organ regeneration and is crucial for identifying factors to trigger hair cell production in mammals. Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 3 different samples: wild type fgf3 mutants and fgf3 siblings.,,pubmed:30681411;pubmed:31488837,,fgf3 sibling,GSM3498553,,source name:neuromast|age:5dpf|tissue:neuromast|genotype:fgf3+/ |strain:fgf3^t26212; ETkrt4:EGFPSqGw57A; Tgpou4f3:GAP GFPs356t,fgf3 sibling,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|genotype:fgf3+/ |strain:fgf3^t26212;ETkrt4:EGFPSqGw57A;Tgpou4f3:GAP GFPs356t,GSM3498553,GSM3498553: fgf3 sibling; Danio rerio; RNA Seq,GSM3498553,,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,GEO Accession:GSM3498553,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP171998,,,possorted_genome_bam_fgf3_sib.bam,10X Genomics bam file,15833733894.0,159936706.0,GSM3498553 r1,0:99,A:4876827108;C:3036857735;G:3248946665;T:4560644612;N:110457774,99,,,,4876827108,3036857735,3248946665,4560644612,110457774,SRX5087287,SRS4099390,SRA817952,GEO,Stowers Institute,1,0.89735,,0.21699,,0.80409,,0.4894,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2018-12-02,Larval,Larval,Lateral Line,Sensory System 50725,SRR8270600,SRX5087286,SRS4099389,SRP171998,PRJNA507898,Single cell RNA Seq reveals Fgf signaling dynamics during sensory hair cell regeneration,GSE123241,Transcriptome Analysis,Loss of sensory hair cells leads to deafness and balance deficiencies. In contrast to mammalian hair cells zebrafish ear and lateral line hair cells regenerate from poorly characterized proliferating support cells. Equally ill defined is the gene regulatory network underlying the progression of support cells to cycling hair cell progenitors and differentiated hair cells. We used single cell RNA Sequencing scRNA Seq of lateral line sensory organs and uncovered five different support cell types including quiescent and activated stem cells. In silico ordering of support cells along a developmental trajectory identified cells that self renew and new groups of genes required for hair cell differentiation. scRNA Seq analyses of fgf3 mutants in which hair cell regeneration is increased demonstrates that Fgf and Notch signaling inhibit proliferation of support cells in parallel by inhibiting Wnt signaling. Our scRNA Seq analyses set the foundation for mechanistic studies of sensory organ regeneration and is crucial for identifying factors to trigger hair cell production in mammals. Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 3 different samples: wild type fgf3 mutants and fgf3 siblings.,,pubmed:30681411;pubmed:31488837,,WT,GSM3498552,,source name:neuromast|age:5dpf|tissue:neuromast|genotype:WT|strain:ETkrt4:EGFPSqGw57A; Tgpou4f3:GAP GFPs356t,WT,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|genotype:WT|strain:ETkrt4:EGFPSqGw57A;Tgpou4f3:GAP GFPs356t,GSM3498552,GSM3498552: WT; Danio rerio; RNA Seq,GSM3498552,,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,GEO Accession:GSM3498552,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP171998,,,possorted_genome_bam_WT.bam,10X Genomics bam file,32918660478.0,332511722.0,GSM3498552 r1,0:99,A:10031241232;C:6365493396;G:6804558002;T:9674542542;N:42825306,99,,,,10031241232,6365493396,6804558002,9674542542,42825306,SRX5087286,SRS4099389,SRA817952,GEO,Stowers Institute,1,0.90685,,0.26503,,0.79368,,0.47639,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2018-12-02,Larval,Larval,Lateral Line,Sensory System 56605,SRR11031290,SRX7683557,SRS6110537,SRP247442,PRJNA605021,Epithelial planar bipolarity emerges from Notch mediated asymmetric inhibition of Emx2,GSE144827,Transcriptome Analysis,This dataset consists of single cell RNA seq 10X data of neuromast cells from zebrafish larvae. Posterior lateral line neuromast cells were isolated by fluorescence activated cell sorting FACS from the dissociated trunks of Tg[myo6b:actb1 EGFP] transgenic zebrafish larvae expressing the green fluorescent protein EGFP in hair cells and unipotent hair cell progenitors UHCPs and from Tg[Etkrt4:EGFPsqgw57A] larvae expressing EGFP in supporting cells. Overall design: A single cell RNA seq experiment of isolated neuromast cells from zebrafish larvae was performed using 10X Genomics technology to generate single cell transcriptomic profiles of supporting cells and hair cells.,,pubmed:32109392,,hair cells,GSM4298909,,source name:zebrafish neuromast cells|genotype:Tg[myo6b:actb1 EGFP]|source tissue:neuromast|developmental day:7 dpf,hair cells,The 10X Genomics core computational software Cellranger v.2.1.1 was used for alignment and processing of next generation sequencing reads of the scRNA seq data as recommended https://support.10xgenomics.com/single cell gene expression/software/overview/welcome. Genome build: GRCz11 Supplementary files format and content: Raw UMI count matrix feature x cell barcode from 10X Genomics single cell RNAseq experiments. gzipped files contains count matrix in market matrix format cell barcodes as tab delimited text file and feature names as tab delimited text file.,zebrafish neuromast cells,none,Enzymatic dissociation of larval trunks and isolation of green fluorescent protein expressing cells by FAC sorting. Single cell libraries were generated using the ChromiumTM Single cell 3`library and gel bead kit v2 PN #120237 from 10x Genomics. Libraries were sequenced on the HiSeq4000 Illumina with 150bp paired end sequencing. RNA Seq 10X Genomics,Standardized conditions at 28.5°C in the 0 3 x Danieau’s solution.,genotype:Tg[myo6b:actb1 EGFP]|source tissue:neuromast|developmental day:7 dpf,GSM4298909,GSM4298909: hair cells; Danio rerio; RNA Seq,GSM4298909,,1,Enzymatic dissociation of larval trunks and isolation of green fluorescent protein expressing cells by FAC sorting. Single cell libraries were generated using the ChromiumTM Single cell 3`library and gel bead kit v2 PN #120237 from 10x Genomics. Libraries were sequenced on the HiSeq4000 Illumina with 150bp paired end sequencing. RNA Seq 10X Genomics,GEO Accession:GSM4298909,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP247442,,,hair_cells_R2.fastq.gz hair_cells_R1.fastq.gz,fastq fastq,89474157378.0,505503714.0,GSM4298909 r1,0:26 1:151,A:25071099481;C:19794086212;G:19845960995;T:24749018204;N:13992486,26,151,,,25071099481,19794086212,19845960995,24749018204,13992486,SRX7683557,SRS6110537,SRA1038640,GEO,"Research Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München",2,0.00294,0.9352,0.00072,0.07461,0.99358,0.81487,0.46744,0.43177,26,151,T,B,sc-like readlen,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Germany,2020-02-05,Larval,Larval,Lateral Line,Sensory System 56606,SRR11031289,SRX7683556,SRS6110536,SRP247442,PRJNA605021,Epithelial planar bipolarity emerges from Notch mediated asymmetric inhibition of Emx2,GSE144827,Transcriptome Analysis,This dataset consists of single cell RNA seq 10X data of neuromast cells from zebrafish larvae. Posterior lateral line neuromast cells were isolated by fluorescence activated cell sorting FACS from the dissociated trunks of Tg[myo6b:actb1 EGFP] transgenic zebrafish larvae expressing the green fluorescent protein EGFP in hair cells and unipotent hair cell progenitors UHCPs and from Tg[Etkrt4:EGFPsqgw57A] larvae expressing EGFP in supporting cells. Overall design: A single cell RNA seq experiment of isolated neuromast cells from zebrafish larvae was performed using 10X Genomics technology to generate single cell transcriptomic profiles of supporting cells and hair cells.,,pubmed:32109392,,supporting cells,GSM4298908,,source name:zebrafish neuromast cells|genotype:Tg[Etkrt4:EGFPsqgw57A]|source tissue:neuromast|developmental day:7 dpf,supporting cells,The 10X Genomics core computational software Cellranger v.2.1.1 was used for alignment and processing of next generation sequencing reads of the scRNA seq data as recommended https://support.10xgenomics.com/single cell gene expression/software/overview/welcome. Genome build: GRCz11 Supplementary files format and content: Raw UMI count matrix feature x cell barcode from 10X Genomics single cell RNAseq experiments. gzipped files contains count matrix in market matrix format cell barcodes as tab delimited text file and feature names as tab delimited text file.,zebrafish neuromast cells,none,Enzymatic dissociation of larval trunks and isolation of green fluorescent protein expressing cells by FAC sorting. Single cell libraries were generated using the ChromiumTM Single cell 3`library and gel bead kit v2 PN #120237 from 10x Genomics. Libraries were sequenced on the HiSeq4000 Illumina with 150bp paired end sequencing. RNA Seq 10X Genomics,Standardized conditions at 28.5°C in the 0 3 x Danieau’s solution.,genotype:Tg[Etkrt4:EGFPsqgw57A]|source tissue:neuromast|developmental day:7 dpf,GSM4298908,GSM4298908: supporting cells; Danio rerio; RNA Seq,GSM4298908,,1,Enzymatic dissociation of larval trunks and isolation of green fluorescent protein expressing cells by FAC sorting. Single cell libraries were generated using the ChromiumTM Single cell 3`library and gel bead kit v2 PN #120237 from 10x Genomics. Libraries were sequenced on the HiSeq4000 Illumina with 150bp paired end sequencing. RNA Seq 10X Genomics,GEO Accession:GSM4298908,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP247442,,,support_cells_R2.fastq.gz support_cells_R1.fastq.gz,fastq fastq,209506251249.0,1183651137.0,GSM4298908 r1,0:26 1:151,A:59676591457;C:46803384480;G:47908589489;T:55084749523;N:32936300,26,151,,,59676591457,46803384480,47908589489,55084749523,32936300,SRX7683556,SRS6110536,SRA1038640,GEO,"Research Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München",2,0.00206,0.92892,0.00042,0.04303,0.99508,0.86354,0.40312,0.45704,26,151,T,B,sc-like readlen,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Germany,2020-02-05,Larval,Larval,Lateral Line,Sensory System 68516,SRR17900890,SRX14059608,SRS11892528,SRP358576,PRJNA803902,High resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration,GSE196211,Transcriptome Analysis,NOT PROVIDED; REQUESTED Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 7 different samples: homeo 0min post neomycin 30min 1hr 3hr 5hr 10hr,,pubmed:35316618,,10hr post neomycin,GSM5862805,,source name:neuromast|age:5dpf|tissue:neuromast|treatment/time point:10hr post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,10hr post neomycin,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 ensembl release 91 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|treatment/time point:10hr post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,GSM5862805,GSM5862805: 10hr post neomycin; Danio rerio; RNA Seq,GSM5862805 r1,GSM5862805,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,SRP358576,,assembly:danRer10|intentional duplicate,10hr_possorted_genome_bam.bam,10X Genomics bam file,11755529379.0,118742721.0,GSM5862805 r1,0:99,A:3815855652;C:2300159508;G:2649552225;T:2989724050;N:237944,99,,,,3815855652,2300159508,2649552225,2989724050,237944,SRX14059608,SRS11892528,SRA1367859,"Piotrowski Lab, Stowers Institute for Medical Research","Piotrowski Lab, Stowers Institute for Medical Research",1,0.88584,,0.18938,,0.81968,,0.57795,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-02-06,Larval,Larval,Lateral Line,Sensory System 68517,SRR17900891,SRX14059607,SRS11892527,SRP358576,PRJNA803902,High resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration,GSE196211,Transcriptome Analysis,NOT PROVIDED; REQUESTED Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 7 different samples: homeo 0min post neomycin 30min 1hr 3hr 5hr 10hr,,pubmed:35316618,,5hr post neomycin,GSM5862804,,source name:neuromast|age:5dpf|tissue:neuromast|treatment/time point:5hr post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,5hr post neomycin,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 ensembl release 91 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|treatment/time point:5hr post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,GSM5862804,GSM5862804: 5hr post neomycin; Danio rerio; RNA Seq,GSM5862804 r1,GSM5862804,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,SRP358576,,assembly:danRer10|intentional duplicate,5hr_possorted_genome_bam.bam,10X Genomics bam file,10868082885.0,109778615.0,GSM5862804 r1,0:99,A:3267489116;C:2251000050;G:2404850523;T:2944522797;N:220399,99,,,,3267489116,2251000050,2404850523,2944522797,220399,SRX14059607,SRS11892527,SRA1367859,"Piotrowski Lab, Stowers Institute for Medical Research","Piotrowski Lab, Stowers Institute for Medical Research",1,0.93721,,0.18379,,0.81852,,0.54714,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-02-06,Larval,Larval,Lateral Line,Sensory System 68518,SRR17900892,SRX14059606,SRS11892526,SRP358576,PRJNA803902,High resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration,GSE196211,Transcriptome Analysis,NOT PROVIDED; REQUESTED Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 7 different samples: homeo 0min post neomycin 30min 1hr 3hr 5hr 10hr,,pubmed:35316618,,3hr post neomycin,GSM5862803,,source name:neuromast|age:5dpf|tissue:neuromast|treatment/time point:3hr post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,3hr post neomycin,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 ensembl release 91 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|treatment/time point:3hr post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,GSM5862803,GSM5862803: 3hr post neomycin; Danio rerio; RNA Seq,GSM5862803 r1,GSM5862803,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,SRP358576,,assembly:danRer10|intentional duplicate,3hr_possorted_genome_bam.bam,10X Genomics bam file,30818123028.0,311294172.0,GSM5862803 r1,0:99,A:9348916523;C:6246488965;G:6684733854;T:8511548264;N:26435422,99,,,,9348916523,6246488965,6684733854,8511548264,26435422,SRX14059606,SRS11892526,SRA1367859,"Piotrowski Lab, Stowers Institute for Medical Research","Piotrowski Lab, Stowers Institute for Medical Research",1,0.92667,,0.21879,,0.80736,,0.52626,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-02-06,Larval,Larval,Lateral Line,Sensory System 68519,SRR17900893,SRX14059605,SRS11892525,SRP358576,PRJNA803902,High resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration,GSE196211,Transcriptome Analysis,NOT PROVIDED; REQUESTED Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 7 different samples: homeo 0min post neomycin 30min 1hr 3hr 5hr 10hr,,pubmed:35316618,,1hr post neomycin,GSM5862802,,source name:neuromast|age:5dpf|tissue:neuromast|treatment/time point:1hr post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,1hr post neomycin,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 ensembl release 91 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|treatment/time point:1hr post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,GSM5862802,GSM5862802: 1hr post neomycin; Danio rerio; RNA Seq,GSM5862802 r1,GSM5862802,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,SRP358576,,assembly:danRer10|intentional duplicate,1hr_possorted_genome_bam.bam,10X Genomics bam file,17016505902.0,171883898.0,GSM5862802 r1,0:99,A:5220935048;C:3448280771;G:3717726451;T:4625338927;N:4224705,99,,,,5220935048,3448280771,3717726451,4625338927,4224705,SRX14059605,SRS11892525,SRA1367859,"Piotrowski Lab, Stowers Institute for Medical Research","Piotrowski Lab, Stowers Institute for Medical Research",1,0.92365,,0.21642,,0.83339,,0.55101,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-02-06,Larval,Larval,Lateral Line,Sensory System 68520,SRR17900894,SRX14059604,SRS11892524,SRP358576,PRJNA803902,High resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration,GSE196211,Transcriptome Analysis,NOT PROVIDED; REQUESTED Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 7 different samples: homeo 0min post neomycin 30min 1hr 3hr 5hr 10hr,,pubmed:35316618,,30min post neomycin,GSM5862801,,source name:neuromast|age:5dpf|tissue:neuromast|treatment/time point:30min post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,30min post neomycin,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 ensembl release 91 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|treatment/time point:30min post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,GSM5862801,GSM5862801: 30min post neomycin; Danio rerio; RNA Seq,GSM5862801 r1,GSM5862801,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,SRP358576,,assembly:danRer10|intentional duplicate,30min_possorted_genome_bam.bam,10X Genomics bam file,15228653913.0,153824787.0,GSM5862801 r1,0:99,A:4774203586;C:3007224980;G:3233337864;T:4213847766;N:39717,99,,,,4774203586,3007224980,3233337864,4213847766,39717,SRX14059604,SRS11892524,SRA1367859,"Piotrowski Lab, Stowers Institute for Medical Research","Piotrowski Lab, Stowers Institute for Medical Research",1,0.92255,,0.24454,,0.83128,,0.55089,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-02-06,Larval,Larval,Lateral Line,Sensory System 68521,SRR17900895,SRX14059603,SRS11892523,SRP358576,PRJNA803902,High resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration,GSE196211,Transcriptome Analysis,NOT PROVIDED; REQUESTED Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 7 different samples: homeo 0min post neomycin 30min 1hr 3hr 5hr 10hr,,pubmed:35316618,,0min post neomycin,GSM5862800,,source name:neuromast|age:5dpf|tissue:neuromast|treatment/time point:0min post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,0min post neomycin,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 ensembl release 91 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|treatment/time point:0min post neomycin|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,GSM5862800,GSM5862800: 0min post neomycin; Danio rerio; RNA Seq,GSM5862800 r1,GSM5862800,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,SRP358576,,assembly:danRer10|intentional duplicate,0min_possorted_genome_bam.bam,10X Genomics bam file,16733371941.0,169023959.0,GSM5862800 r1,0:99,A:5247307437;C:3263851520;G:3538128507;T:4684038782;N:45695,99,,,,5247307437,3263851520,3538128507,4684038782,45695,SRX14059603,SRS11892523,SRA1367859,"Piotrowski Lab, Stowers Institute for Medical Research","Piotrowski Lab, Stowers Institute for Medical Research",1,0.91636,,0.24042,,0.82875,,0.54973,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-02-06,Larval,Larval,Lateral Line,Sensory System 68522,SRR17900896,SRX14059602,SRS11892522,SRP358576,PRJNA803902,High resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration,GSE196211,Transcriptome Analysis,NOT PROVIDED; REQUESTED Overall design: Single cell RNA sequecing on FACS sorted cells from the zebrafish neuromast in 5df embryos in 7 different samples: homeo 0min post neomycin 30min 1hr 3hr 5hr 10hr,,pubmed:35316618,,homeo,GSM5862799,,source name:neuromast|age:5dpf|tissue:neuromast|treatment/time point:homeo|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,homeo,Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome GRCz10 ensembl release 91 using the Cell Ranger version 2.1.1 pipeline from 10x genomics using the default settings. Genome build: danRer10 Supplementary files format and content: h5 files contain the UMI counts for each cell.,neuromast,,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,age:5dpf|tissue:neuromast|treatment/time point:homeo|strain:Tgmyo6b:H2B mScarlet I/Et20:GFP,GSM5862799,GSM5862799: homeo; Danio rerio; RNA Seq,GSM5862799 r1,GSM5862799,1,Presumptive neuromast cells GFP+ were isolated by FACS. Standard 10x genomics scRNA seq protocol using v2 chemistry Droplet based scRNA seq,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,SRP358576,,assembly:danRer10|intentional duplicate,homeo_possorted_genome_bam.bam,10X Genomics bam file,15150832686.0,153038714.0,GSM5862799 r1,0:99,A:4484040403;C:3115374217;G:3333967460;T:4132413883;N:85036723,99,,,,4484040403,3115374217,3333967460,4132413883,85036723,SRX14059602,SRS11892522,SRA1367859,"Piotrowski Lab, Stowers Institute for Medical Research","Piotrowski Lab, Stowers Institute for Medical Research",1,0.93926,,0.18875,,0.80762,,0.50625,,99,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-02-06,Larval,Larval,Lateral Line,Sensory System