rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 53291,SRR9849281,SRX6603894,SRS5168943,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 5 mpf replicate 5,wt 14560 b kidney,,strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 5 mpf replicate 5,wt 14560 b kidney,wt 14560 b kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14560-b-kidney_merged_S17-1.fastq.gz wt-14560-b-kidney_merged_S17-2.fastq.gz,fastq fastq,3653995129.0,24359221.0,wt 14560 b kidney merged S17 1.fastq.gz,0:75.01 1:74.99,A:929157328;C:876547415;G:871160744;T:954251039;N:22878603,75,74,,,929157328,876547415,871160744,954251039,22878603,SRX6603894,SRS5168943,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95086,0.95525,0.0929,0.09179,0.72099,0.72226,0.54133,0.53922,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53292,SRR9849193,SRX6603893,SRS5168942,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 5 mpf replicate 4,wt 14560 g kidney,,strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 4|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 5 mpf replicate 4,wt 14560 g kidney,wt 14560 g kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14560-g-kidney_merged_S77-1.fastq.gz wt-14560-g-kidney_merged_S77-2.fastq.gz,fastq fastq,3573615196.0,24717802.0,wt 14560 g kidney merged S77 1.fastq.gz,0:72.27 1:72.31,A:898296425;C:813436864;G:804954936;T:918903824;N:138023147,72,72,,,898296425,813436864,804954936,918903824,138023147,SRX6603893,SRS5168942,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95022,0.95352,0.10595,0.10566,0.7149,0.71589,0.55515,0.55564,35,35,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53293,SRR9849199,SRX6603892,SRS5168941,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 3 mpf replicate 3,kl 14744 g kidney,,strain:AB|age:3months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 3 mpf replicate 3,kl 14744 g kidney,kl 14744 g kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14744-g-kidney_merged_S83-1.fastq.gz kl-14744-g-kidney_merged_S83-2.fastq.gz,fastq fastq,3737995505.0,25138545.0,kl 14744 g kidney merged S83 1.fastq.gz,0:74.34 1:74.35,A:957928510;C:878559278;G:871082168;T:980068971;N:50356578,74,74,,,957928510,878559278,871082168,980068971,50356578,SRX6603892,SRS5168941,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94562,0.94982,0.09916,0.09805,0.71585,0.71727,0.53455,0.52965,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53294,SRR9849194,SRX6603891,SRS5168940,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 3 mpf replicate 2,kl 14744 e kidney,,strain:AB|age:3months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 3 mpf replicate 2,kl 14744 e kidney,kl 14744 e kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14744-e-kidney_merged_S59-1.fastq.gz kl-14744-e-kidney_merged_S59-2.fastq.gz,fastq fastq,3189473322.0,21535442.0,kl 14744 e kidney merged S59 1.fastq.gz,0:74.05 1:74.05,A:823511818;C:738062888;G:732498035;T:841940599;N:53459982,74,74,,,823511818,738062888,732498035,841940599,53459982,SRX6603891,SRS5168940,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94678,0.95071,0.09344,0.09218,0.71971,0.72129,0.54757,0.54618,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53295,SRR9849198,SRX6603890,SRS5168939,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 3 mpf replicate 1,kl 14744 a kidney,,strain:AB|age:3months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 3 mpf replicate 1,kl 14744 a kidney,kl 14744 a kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14744-a-kidney_merged_S58-1.fastq.gz kl-14744-a-kidney_merged_S58-2.fastq.gz,fastq fastq,3553107262.0,24182301.0,kl 14744 a kidney merged S58 1.fastq.gz,0:73.47 1:73.46,A:902558501;C:824321758;G:817836028;T:922899545;N:85491430,73,73,,,902558501,824321758,817836028,922899545,85491430,SRX6603890,SRS5168939,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95045,0.95329,0.09674,0.09571,0.72072,0.72135,0.52747,0.53937,74,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53296,SRR9849195,SRX6603889,SRS5168938,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 5 mpf replicate 8,wt 14560 h kidney,,strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 8|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 5 mpf replicate 8,wt 14560 h kidney,wt 14560 h kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14560-h-kidney_merged_S89-1.fastq.gz wt-14560-h-kidney_merged_S89-2.fastq.gz,fastq fastq,3309513073.0,22153246.0,wt 14560 h kidney merged S89 1.fastq.gz,0:74.72 1:74.67,A:790892918;C:836461180;G:837452371;T:813600384;N:31106220,74,74,,,790892918,836461180,837452371,813600384,31106220,SRX6603889,SRS5168938,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.96472,0.96761,0.054,0.05438,0.71175,0.71348,0.51051,0.51195,75,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53297,SRR9849196,SRX6603888,SRS5168937,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 3 mpf replicate 4,kl 14744 b kidney,,strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 4|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 3 mpf replicate 4,kl 14744 b kidney,kl 14744 b kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14744-b-kidney_merged_S70-1.fastq.gz kl-14744-b-kidney_merged_S70-2.fastq.gz,fastq fastq,3696398655.0,24993764.0,kl 14744 b kidney merged S70 1.fastq.gz,0:73.95 1:73.94,A:928283187;C:880520024;G:868819514;T:952460036;N:66315894,73,73,,,928283187,880520024,868819514,952460036,66315894,SRX6603888,SRS5168937,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94725,0.95062,0.08433,0.08251,0.7139,0.71334,0.52496,0.52625,65,65,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53298,SRR9849197,SRX6603887,SRS5168935,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 3 mpf replicate 5,kl 14744 d kidney,,strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 3 mpf replicate 5,kl 14744 d kidney,kl 14744 d kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14744-d-kidney_merged_S94-1.fastq.gz kl-14744-d-kidney_merged_S94-2.fastq.gz,fastq fastq,3235564566.0,21704627.0,kl 14744 d kidney merged S94 1.fastq.gz,0:74.55 1:74.53,A:808568448;C:783575935;G:776803253;T:830140556;N:36476374,74,74,,,808568448,783575935,776803253,830140556,36476374,SRX6603887,SRS5168935,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95065,0.95457,0.07932,0.0782,0.69844,0.69852,0.52161,0.52384,75,75,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53299,SRR9849200,SRX6603886,SRS5168936,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 5 mpf replicate 7,kl 14563 b kidney,,strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 5 mpf replicate 7,kl 14563 b kidney,kl 14563 b kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14563-b-kidney_merged_S23-1.fastq.gz kl-14563-b-kidney_merged_S23-2.fastq.gz,fastq fastq,3841497499.0,25646923.0,kl 14563 b kidney merged S23 1.fastq.gz,0:74.90 1:74.89,A:999048603;C:899322616;G:891418096;T:1023530696;N:28177488,74,74,,,999048603,899322616,891418096,1023530696,28177488,SRX6603886,SRS5168936,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94562,0.94897,0.098,0.09621,0.71977,0.7209,0.54321,0.53814,76,75,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53300,SRR9849206,SRX6603885,SRS5168934,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 5 mpf replicate 8,kl 14563 c kidney,,strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 8|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 5 mpf replicate 8,kl 14563 c kidney,kl 14563 c kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14563-c-kidney_merged_S35-1.fastq.gz kl-14563-c-kidney_merged_S35-2.fastq.gz,fastq fastq,4215569581.0,28089663.0,kl 14563 c kidney merged S35 1.fastq.gz,0:75.05 1:75.03,A:1122288966;C:970063144;G:955510223;T:1146500593;N:21206655,75,75,,,1122288966,970063144,955510223,1146500593,21206655,SRX6603885,SRS5168934,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94171,0.94373,0.11361,0.11133,0.70136,0.7025,0.49345,0.51472,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53301,SRR9849201,SRX6603884,SRS5168933,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 5 mpf replicate 5,kl 14562 b kidney,,strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 5 mpf replicate 5,kl 14562 b kidney,kl 14562 b kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14562-b-kidney_merged_S18-1.fastq.gz kl-14562-b-kidney_merged_S18-2.fastq.gz,fastq fastq,3972193490.0,27155081.0,kl 14562 b kidney merged S18 1.fastq.gz,0:73.14 1:73.14,A:987358107;C:933387787;G:924448548;T:1015576411;N:111422637,73,73,,,987358107,933387787,924448548,1015576411,111422637,SRX6603884,SRS5168933,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94949,0.95176,0.08272,0.08121,0.7166,0.71705,0.53783,0.53989,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53302,SRR9849205,SRX6603883,SRS5168932,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 5 mpf replicate 6,kl 14562 d kidney,,strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 5 mpf replicate 6,kl 14562 d kidney,kl 14562 d kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14562-d-kidney_merged_S42-1.fastq.gz kl-14562-d-kidney_merged_S42-2.fastq.gz,fastq fastq,3526368194.0,23516128.0,kl 14562 d kidney merged S42 1.fastq.gz,0:75.00 1:74.95,A:869319867;C:871562247;G:869066601;T:893717214;N:22702265,75,74,,,869319867,871562247,869066601,893717214,22702265,SRX6603883,SRS5168932,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.96175,0.96369,0.06361,0.06218,0.70905,0.71157,0.51899,0.52384,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53303,SRR9849202,SRX6603882,SRS5168931,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 5 mpf replicate 3,kl 14563 a kidney,,strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 5 mpf replicate 3,kl 14563 a kidney,kl 14563 a kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14563-a-kidney_merged_S11-1.fastq.gz kl-14563-a-kidney_merged_S11-2.fastq.gz,fastq fastq,4326402378.0,29503938.0,kl 14563 a kidney merged S11 1.fastq.gz,0:73.31 1:73.33,A:1085969692;C:1017480039;G:1010619557;T:1105911425;N:106421665,73,73,,,1085969692,1017480039,1010619557,1105911425,106421665,SRX6603882,SRS5168931,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94169,0.94864,0.08735,0.0856,0.74018,0.74063,0.532,0.54233,35,35,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53304,SRR9849203,SRX6603881,SRS5168930,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 5 mpf replicate 1,kl 14562 a kidney,,strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 5 mpf replicate 1,kl 14562 a kidney,kl 14562 a kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14562-a-kidney_merged_S6-1.fastq.gz kl-14562-a-kidney_merged_S6-2.fastq.gz,fastq fastq,4980501349.0,34004455.0,kl 14562 a kidney merged S6 1.fastq.gz,0:73.23 1:73.24,A:1251556852;C:1167350759;G:1159943717;T:1277350171;N:124299850,73,73,,,1251556852,1167350759,1159943717,1277350171,124299850,SRX6603881,SRS5168930,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94619,0.94971,0.08823,0.08683,0.72807,0.72796,0.51972,0.52145,35,35,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53305,SRR9849204,SRX6603880,SRS5168929,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 5 mpf replicate 4,kl 14563 d kidney,,strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 4|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 5 mpf replicate 4,kl 14563 d kidney,kl 14563 d kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14563-d-kidney_merged_S47-1.fastq.gz kl-14563-d-kidney_merged_S47-2.fastq.gz,fastq fastq,6869441307.0,56338972.0,kl 14563 d kidney merged S47 1.fastq.gz,0:60.76 1:61.17,A:1348721440;C:1368998259;G:1366726420;T:1380954564;N:1404040624,60,61,,,1348721440,1368998259,1366726420,1380954564,1404040624,SRX6603880,SRS5168929,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.96257,0.96453,0.06751,0.06671,0.75548,0.75607,0.51978,0.52832,74,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53306,SRR9849207,SRX6603879,SRS5168928,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 5 mpf replicate 2,kl 14562 c kidney,,strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 5 mpf replicate 2,kl 14562 c kidney,kl 14562 c kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14562-c-kidney_merged_S30-1.fastq.gz kl-14562-c-kidney_merged_S30-2.fastq.gz,fastq fastq,3320801196.0,22339756.0,kl 14562 c kidney merged S30 1.fastq.gz,0:74.33 1:74.32,A:856878404;C:774178244;G:769653230;T:875894981;N:44196337,74,74,,,856878404,774178244,769653230,875894981,44196337,SRX6603879,SRS5168928,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95411,0.95756,0.08333,0.08245,0.7162,0.71638,0.56576,0.55653,74,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53307,SRR9849213,SRX6603878,SRS5168927,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 3 mpf replicate 6,kl 14744 f kidney,,strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 3 mpf replicate 6,kl 14744 f kidney,kl 14744 f kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14744-f-kidney_merged_S71-1.fastq.gz kl-14744-f-kidney_merged_S71-2.fastq.gz,fastq fastq,2801226765.0,18731091.0,kl 14744 f kidney merged S71 1.fastq.gz,0:74.79 1:74.76,A:720950274;C:664580809;G:653567624;T:738645101;N:23482957,74,74,,,720950274,664580809,653567624,738645101,23482957,SRX6603878,SRS5168927,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94692,0.95016,0.10024,0.09917,0.69781,0.69745,0.53063,0.53615,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53308,SRR9849208,SRX6603877,SRS5168926,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,αKlotho kidney 3 mpf replicate 7,kl 14744 h kidney,,strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal,,,,,,,,,Klotho kidney 3 mpf replicate 7,kl 14744 h kidney,kl 14744 h kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,kl-14744-h-kidney_merged_S95-1.fastq.gz kl-14744-h-kidney_merged_S95-2.fastq.gz,fastq fastq,3606112938.0,24298531.0,kl 14744 h kidney merged S95 1.fastq.gz,0:74.21 1:74.20,A:907594636;C:863534560;G:852256795;T:929423242;N:53303705,74,74,,,907594636,863534560,852256795,929423242,53303705,SRX6603877,SRS5168926,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95343,0.95612,0.07895,0.07817,0.70944,0.71023,0.54468,0.54382,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53309,SRR9849212,SRX6603876,SRS5168925,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 5 mpf replicate 2,wt 14560 c kidney,,strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 5 mpf replicate 2,wt 14560 c kidney,wt 14560 c kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14560-c-kidney_merged_S29-1.fastq.gz wt-14560-c-kidney_merged_S29-2.fastq.gz,fastq fastq,3091657773.0,21774524.0,wt 14560 c kidney merged S29 1.fastq.gz,0:70.96 1:71.03,A:763113194;C:693515052;G:687380502;T:778507279;N:169141746,70,71,,,763113194,693515052,687380502,778507279,169141746,SRX6603876,SRS5168925,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95062,0.95349,0.09247,0.09119,0.72754,0.72863,0.53643,0.54181,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53310,SRR9849209,SRX6603875,SRS5168924,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 5 mpf replicate 3,wt 14560 e kidney,,strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 5 mpf replicate 3,wt 14560 e kidney,wt 14560 e kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14560-e-kidney_merged_S53-1.fastq.gz wt-14560-e-kidney_merged_S53-2.fastq.gz,fastq fastq,3242753931.0,21639832.0,wt 14560 e kidney merged S53 1.fastq.gz,0:74.93 1:74.92,A:843085674;C:760074682;G:756945733;T:860752379;N:21895463,74,74,,,843085674,760074682,756945733,860752379,21895463,SRX6603875,SRS5168924,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95058,0.95272,0.0982,0.09719,0.71707,0.71825,0.5497,0.53642,75,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53311,SRR9849210,SRX6603874,SRS5168923,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 3 mpf replicate 7,wt 14746 g kidney,,strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 3 mpf replicate 7,wt 14746 g kidney,wt 14746 g kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14746-g-kidney_merged_S22-1.fastq.gz wt-14746-g-kidney_merged_S22-2.fastq.gz,fastq fastq,3928427553.0,26318173.0,wt 14746 g kidney merged S22 1.fastq.gz,0:74.63 1:74.64,A:1019611627;C:916883865;G:906743219;T:1044351913;N:40836929,74,74,,,1019611627,916883865,906743219,1044351913,40836929,SRX6603874,SRS5168923,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94281,0.94563,0.09937,0.0975,0.71478,0.71585,0.54524,0.5444,76,74,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53312,SRR9849211,SRX6603873,SRS5168922,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 3 mpf replicate 6,wt 14746 d kidney,,strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 3 mpf replicate 6,wt 14746 d kidney,wt 14746 d kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14746-d-kidney_merged_S81-2.fastq.gz wt-14746-d-kidney_merged_S81-1.fastq.gz,fastq fastq,3235839633.0,21863802.0,wt 14746 d kidney merged S81 1.fastq.gz,0:73.99 1:74.01,A:819433052;C:762877585;G:759574301;T:837534821;N:56419874,73,74,,,819433052,762877585,759574301,837534821,56419874,SRX6603873,SRS5168922,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94986,0.95295,0.09789,0.09661,0.72109,0.72344,0.5002,0.53439,75,75,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53313,SRR9849214,SRX6603872,SRS5168921,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 3 mpf replicate 8,wt 14746 i kidney,,strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 8|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 3 mpf replicate 8,wt 14746 i kidney,wt 14746 i kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14746-i-kidney_merged_S46-1.fastq.gz wt-14746-i-kidney_merged_S46-2.fastq.gz,fastq fastq,3377453037.0,23023990.0,wt 14746 i kidney merged S46 1.fastq.gz,0:73.35 1:73.34,A:836450075;C:803489724;G:795497926;T:858148995;N:83866317,73,73,,,836450075,803489724,795497926,858148995,83866317,SRX6603872,SRS5168921,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95192,0.95558,0.0795,0.07926,0.70589,0.70619,0.52743,0.52432,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53314,SRR9849220,SRX6603871,SRS5168920,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 5 mpf replicate 1,wt 14560 a kidney,,strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 5 mpf replicate 1,wt 14560 a kidney,wt 14560 a kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14560-a-kidney_merged_S5-1.fastq.gz wt-14560-a-kidney_merged_S5-2.fastq.gz,fastq fastq,3803097028.0,29032173.0,wt 14560 a kidney merged S5 1.fastq.gz,0:65.34 1:65.66,A:880043540;C:764446357;G:759630921;T:898342437;N:500633773,65,65,,,880043540,764446357,759630921,898342437,500633773,SRX6603871,SRS5168920,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94594,0.94896,0.10801,0.10661,0.74292,0.74379,0.56139,0.56212,76,75,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53315,SRR9849215,SRX6603870,SRS5168919,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 3 mpf replicate 2,wt 14746 e kidney,,strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 3 mpf replicate 2,wt 14746 e kidney,wt 14746 e kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14746-e-kidney_merged_S93-1.fastq.gz wt-14746-e-kidney_merged_S93-2.fastq.gz,fastq fastq,3389715259.0,23030176.0,wt 14746 e kidney merged S93 1.fastq.gz,0:73.58 1:73.60,A:845566798;C:806510240;G:801049741;T:861758601;N:74829879,73,73,,,845566798,806510240,801049741,861758601,74829879,SRX6603870,SRS5168919,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.96201,0.96536,0.07181,0.0708,0.73594,0.73628,0.5625,0.57023,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53316,SRR9849219,SRX6603869,SRS5168918,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 3 mpf replicate 3,wt 14746 f kidney,,strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 3 mpf replicate 3,wt 14746 f kidney,wt 14746 f kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14746-f-kidney_merged_S10-1.fastq.gz wt-14746-f-kidney_merged_S10-2.fastq.gz,fastq fastq,5697834151.0,38061527.0,wt 14746 f kidney merged S10 1.fastq.gz,0:74.86 1:74.84,A:1488491305;C:1328009157;G:1311260167;T:1524663985;N:45409537,74,74,,,1488491305,1328009157,1311260167,1524663985,45409537,SRX6603869,SRS5168918,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94873,0.9525,0.0943,0.09336,0.71709,0.71741,0.53907,0.55371,76,75,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53317,SRR9849216,SRX6603868,SRS5168917,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 3 mpf replicate 4,wt 14746 h kidney,,strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 4|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 3 mpf replicate 4,wt 14746 h kidney,wt 14746 h kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14746-h-kidney_merged_S34-1.fastq.gz wt-14746-h-kidney_merged_S34-2.fastq.gz,fastq fastq,4802949250.0,32003702.0,wt 14746 h kidney merged S34 1.fastq.gz,0:75.05 1:75.02,A:1247408978;C:1132423247;G:1119030906;T:1277389500;N:26696619,75,75,,,1247408978,1132423247,1119030906,1277389500,26696619,SRX6603868,SRS5168917,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94868,0.95207,0.1008,0.1002,0.70654,0.70583,0.543,0.53646,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53318,SRR9849217,SRX6603867,SRS5168916,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 3 mpf replicate 1,wt 14746 c kidney,,strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 3 mpf replicate 1,wt 14746 c kidney,wt 14746 c kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14746-c-kidney_merged_S69-1.fastq.gz wt-14746-c-kidney_merged_S69-2.fastq.gz,fastq fastq,2926155789.0,19616550.0,wt 14746 c kidney merged S69 1.fastq.gz,0:74.59 1:74.58,A:738508985;C:703442172;G:697795154;T:755346949;N:31062529,74,74,,,738508985,703442172,697795154,755346949,31062529,SRX6603867,SRS5168916,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.95391,0.95737,0.08073,0.08025,0.72273,0.72265,0.50256,0.53781,76,75,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53319,SRR9849218,SRX6603866,SRS5168915,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 3 mpf replicate 5,wt 14746 b kidney,,strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 3 mpf replicate 5,wt 14746 b kidney,wt 14746 b kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14746-b-kidney_merged_S57-1.fastq.gz wt-14746-b-kidney_merged_S57-2.fastq.gz,fastq fastq,3580693879.0,24148496.0,wt 14746 b kidney merged S57 1.fastq.gz,0:74.13 1:74.15,A:906877863;C:847589932;G:842258575;T:927440495;N:56527014,74,74,,,906877863,847589932,842258575,927440495,56527014,SRX6603866,SRS5168915,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.94558,0.95003,0.09575,0.09468,0.72099,0.72192,0.52455,0.51579,75,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53377,SRR9849279,SRX6603808,SRS5168857,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 5 mpf replicate 7,wt 14560 f kidney,,strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 5 mpf replicate 7,wt 14560 f kidney,wt 14560 f kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14560-f-kidney_merged_S65-1.fastq.gz wt-14560-f-kidney_merged_S65-2.fastq.gz,fastq fastq,3381175351.0,22760562.0,wt 14560 f kidney merged S65 1.fastq.gz,0:74.29 1:74.26,A:834174555;C:827862556;G:819581002;T:856481808;N:43075430,74,74,,,834174555,827862556,819581002,856481808,43075430,SRX6603808,SRS5168857,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.96026,0.96284,0.07018,0.06939,0.69662,0.69647,0.48686,0.48311,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 53378,SRR9849280,SRX6603807,SRS5168856,SRP216556,PRJNA556842,aKlotho regulates age associated vascular calcification and lifespan in zebrafish,PRJNA556842,Transcriptome Analysis,The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.,,,,wildtype kidney 5 mpf replicate 6,wt 14560 d kidney,,strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal,,,,,,,,,wildtype kidney 5 mpf replicate 6,wt 14560 d kidney,wt 14560 d kidney,Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP216556,,,wt-14560-d-kidney_merged_S41-1.fastq.gz wt-14560-d-kidney_merged_S41-2.fastq.gz,fastq fastq,3260080878.0,21943002.0,wt 14560 d kidney merged S41 1.fastq.gz,0:74.31 1:74.26,A:781693169;C:817346676;G:813482706;T:801873503;N:45684824,74,74,,,781693169,817346676,813482706,801873503,45684824,SRX6603807,SRS5168856,SRA927891,Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics,Novartis Institutes for Biomedical Research,2,0.96382,0.96558,0.05582,0.05496,0.72245,0.72342,0.50059,0.50428,76,76,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2019-07-27,Adult,Adult,Kidney,Renal System 62687,SRR13319605,SRX9747096,SRS7934718,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,Mut 10d k1,Mut 10d k1,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:15 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,Mut 10d k1,Mut 10d k1,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,Mut_10d_k1.raw_1.fastq.gz Mut_10d_k1.raw_2.fastq.gz,fastq fastq,6906287100.0,23020957.0,Mut 10d k1.raw 1.fastq.gz,0:150 1:150,A:1908295029;C:1526919757;G:1627893432;T:1843041112;N:137770,150,150,,,1908295029,1526919757,1627893432,1843041112,137770,SRX9747096,SRS7934718,SRA1178602,southwest university|college of fisheries,southwest university,2,0.94038,0.93872,0.08297,0.08272,0.71382,0.71539,0.54124,0.54083,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62688,SRR13319606,SRX9747095,SRS7934719,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,WT 10d k3,WT 10d k3,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:14 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,WT 10d k3,WT 10d k3,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,WT_10d_k3.raw_1.fastq.gz WT_10d_k3.raw_2.fastq.gz,fastq fastq,7159590600.0,23865302.0,WT 10d k3.raw 1.fastq.gz,0:150 1:150,A:1984034638;C:1574065265;G:1674336416;T:1927009990;N:144291,150,150,,,1984034638,1574065265,1674336416,1927009990,144291,SRX9747095,SRS7934719,SRA1178602,southwest university|college of fisheries,southwest university,2,0.92946,0.92803,0.08386,0.08381,0.71052,0.71074,0.51146,0.51825,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62689,SRR13319607,SRX9747094,SRS7934714,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,WT 10d k2,WT 10d k2,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:13 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,WT 10d k2,WT 10d k2,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,WT_10d_k2.raw_1.fastq.gz WT_10d_k2.raw_2.fastq.gz,fastq fastq,6354585300.0,21181951.0,WT 10d k2.raw 1.fastq.gz,0:150 1:150,A:1749380892;C:1406921465;G:1504331323;T:1693821680;N:129940,150,150,,,1749380892,1406921465,1504331323,1693821680,129940,SRX9747094,SRS7934714,SRA1178602,southwest university|college of fisheries,southwest university,2,0.93216,0.9306,0.0766,0.07651,0.70847,0.70936,0.53878,0.53632,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62690,SRR13319608,SRX9747093,SRS7934720,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,WT 10d k1,WT 10d k1,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:12 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,WT 10d k1,WT 10d k1,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,WT_10d_k1.raw_1.fastq.gz WT_10d_k1.raw_2.fastq.gz,fastq fastq,8712565800.0,29041886.0,WT 10d k1.raw 1.fastq.gz,0:150 1:150,A:2388117969;C:1934065753;G:2091791049;T:2298416148;N:174881,150,150,,,2388117969,1934065753,2091791049,2298416148,174881,SRX9747093,SRS7934720,SRA1178602,southwest university|college of fisheries,southwest university,2,0.93468,0.93398,0.09783,0.0975,0.69593,0.69649,0.52524,0.52599,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62691,SRR13319609,SRX9747092,SRS7934712,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,Mut 6h k3,Mut 6h k3,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:11 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,Mut 6h k3,Mut 6h k3,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,Mut_6h_k3.raw_1.fastq.gz Mut_6h_k3.raw_2.fastq.gz,fastq fastq,6821167500.0,22737225.0,Mut 6h k3.raw 1.fastq.gz,0:150 1:150,A:1903976437;C:1488858303;G:1566082857;T:1862227563;N:22340,150,150,,,1903976437,1488858303,1566082857,1862227563,22340,SRX9747092,SRS7934712,SRA1178602,southwest university|college of fisheries,southwest university,2,0.93422,0.93209,0.09882,0.09902,0.71784,0.71892,0.53469,0.5464,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62692,SRR13319610,SRX9747091,SRS7934717,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,Mut 6h k2,Mut 6h k2,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:10 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,Mut 6h k2,Mut 6h k2,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,Mut_6h_k2.raw_1.fastq.gz Mut_6h_k2.raw_2.fastq.gz,fastq fastq,6614007900.0,22046693.0,Mut 6h k2.raw 1.fastq.gz,0:150 1:150,A:1836116506;C:1454221417;G:1524536821;T:1799111318;N:21838,150,150,,,1836116506,1454221417,1524536821,1799111318,21838,SRX9747091,SRS7934717,SRA1178602,southwest university|college of fisheries,southwest university,2,0.93405,0.93165,0.09928,0.0998,0.71378,0.71405,0.53405,0.53636,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62693,SRR13319611,SRX9747090,SRS7934716,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,Mut 6h k1,Mut 6h k1,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:9 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,Mut 6h k1,Mut 6h k1,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,Mut_6h_k1.raw_1.fastq.gz Mut_6h_k1.raw_2.fastq.gz,fastq fastq,6856096500.0,22853655.0,Mut 6h k1.raw 1.fastq.gz,0:150 1:150,A:1914687245;C:1494001561;G:1567738680;T:1879647930;N:21084,150,150,,,1914687245,1494001561,1567738680,1879647930,21084,SRX9747090,SRS7934716,SRA1178602,southwest university|college of fisheries,southwest university,2,0.92882,0.92611,0.10791,0.10832,0.71378,0.71427,0.53012,0.52707,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62694,SRR13319612,SRX9747089,SRS7934713,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,WT 6h k3,WT 6h k3,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:8 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,WT 6h k3,WT 6h k3,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,WT_6h_k3.raw_1.fastq.gz WT_6h_k3.raw_2.fastq.gz,fastq fastq,7194856500.0,23982855.0,WT 6h k3.raw 1.fastq.gz,0:150 1:150,A:2000223263;C:1580258499;G:1657803759;T:1956549208;N:21771,150,150,,,2000223263,1580258499,1657803759,1956549208,21771,SRX9747089,SRS7934713,SRA1178602,southwest university|college of fisheries,southwest university,2,0.93193,0.92986,0.09623,0.09609,0.70907,0.71029,0.53843,0.53754,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62695,SRR13319613,SRX9747088,SRS7934715,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,Mut 10d k3,Mut 10d k3,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:17 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,Mut 10d k3,Mut 10d k3,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,Mut_10d_k3.raw_1.fastq.gz Mut_10d_k3.raw_2.fastq.gz,fastq fastq,7859046900.0,26196823.0,Mut 10d k3.raw 1.fastq.gz,0:150 1:150,A:2162835249;C:1743162639;G:1857995098;T:2094896175;N:157739,150,150,,,2162835249,1743162639,1857995098,2094896175,157739,SRX9747088,SRS7934715,SRA1178602,southwest university|college of fisheries,southwest university,2,0.93831,0.93732,0.0836,0.08331,0.69988,0.70132,0.54542,0.54383,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62696,SRR13319614,SRX9747087,SRS7934710,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,Mut 10d k2,Mut 10d k2,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:16 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,Mut 10d k2,Mut 10d k2,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,Mut_10d_k2.raw_1.fastq.gz Mut_10d_k2.raw_2.fastq.gz,fastq fastq,6163552500.0,20545175.0,Mut 10d k2.raw 1.fastq.gz,0:150 1:150,A:1690284188;C:1371212067;G:1471613432;T:1630318376;N:124437,150,150,,,1690284188,1371212067,1471613432,1630318376,124437,SRX9747087,SRS7934710,SRA1178602,southwest university|college of fisheries,southwest university,2,0.94142,0.93953,0.07637,0.07599,0.7123,0.71323,0.54883,0.55044,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62697,SRR13319615,SRX9747086,SRS7934709,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,WT 6h k2,WT 6h k2,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,WT 6h k2,WT 6h k2,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,WT_6h_k2.raw_1.fastq.gz WT_6h_k2.raw_2.fastq.gz,fastq fastq,7675977600.0,25586592.0,WT 6h k2.raw 1.fastq.gz,0:150 1:150,A:2123159765;C:1695880551;G:1771133441;T:2085779945;N:23898,150,150,,,2123159765,1695880551,1771133441,2085779945,23898,SRX9747086,SRS7934709,SRA1178602,southwest university|college of fisheries,southwest university,2,0.93307,0.93101,0.09081,0.09046,0.7097,0.71031,0.52694,0.52787,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62698,SRR13319616,SRX9747085,SRS7934711,SRP299583,PRJNA688352,Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,PRJNA688352,Other,A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells,,,,WT 6h k1,WT 6h k1,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:6 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination,WT 6h k1,WT 6h k1,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299583,,,WT_6h_k1.raw_1.fastq.gz WT_6h_k1.raw_2.fastq.gz,fastq fastq,5660758200.0,18869194.0,WT 6h k1.raw 1.fastq.gz,0:150 1:150,A:1560962707;C:1253335257;G:1319974501;T:1526464986;N:20749,150,150,,,1560962707,1253335257,1319974501,1526464986,20749,SRX9747085,SRS7934711,SRA1178602,southwest university|college of fisheries,southwest university,2,0.93079,0.92974,0.09975,0.09988,0.70504,0.70581,0.53219,0.533,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-28,Adult,Adult,Kidney,Renal System 62700,SRR13327930,SRX9755046,SRS7944756,SRP299775,PRJNA688655,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,PRJNA688655,Other,,,,,Mut 0h k3,Mut 0h k3,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:11|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,Mut 0h k3,Mut k3,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299775,,,WT_0h_k3.raw_1.fastq.gz WT_0h_k3.raw_2.fastq.gz,fastq fastq,6675348000.0,22251160.0,WT 0h k3.raw 1.fastq.gz,0:150 1:150,A:1848988411;C:1470625677;G:1544465916;T:1811246237;N:21759,150,150,,,1848988411,1470625677,1544465916,1811246237,21759,SRX9755046,SRS7944756,SRA1178968,southwest university|college of fisheries,southwest university,2,0.86263,0.92706,0.08978,0.0978,0.70502,0.69895,0.55273,0.55505,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-30,Undetermined,Undetermined,Kidney,Renal System 62701,SRR13327931,SRX9755045,SRS7944755,SRP299775,PRJNA688655,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,PRJNA688655,Other,,,,,Mut 0h k2,Mut 0h k2,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:10|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,Mut 0h k2,Mut k2,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299775,,,WT_0h_k2.raw_1.fastq.gz WT_0h_k2.raw_2.fastq.gz,fastq fastq,7165537800.0,23885126.0,WT 0h k2.raw 1.fastq.gz,0:150 1:150,A:1961366053;C:1599856726;G:1683336568;T:1920956264;N:22189,150,150,,,1961366053,1599856726,1683336568,1920956264,22189,SRX9755045,SRS7944755,SRA1178968,southwest university|college of fisheries,southwest university,2,0.93437,0.93173,0.09166,0.09117,0.6997,0.70031,0.5364,0.53886,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-30,Undetermined,Undetermined,Kidney,Renal System 62702,SRR13327932,SRX9755044,SRS7944754,SRP299775,PRJNA688655,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,PRJNA688655,Other,,,,,Mut 0h k1,Mut 0h k1,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:9|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,Mut 0h k1,Mut k1,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299775,,,WT_0h_k1.raw_1.fastq.gz WT_0h_k1.raw_2.fastq.gz,fastq fastq,5953238400.0,19844128.0,WT 0h k1.raw 1.fastq.gz,0:150 1:150,A:1643432335;C:1323053555;G:1377906382;T:1608824934;N:21194,150,150,,,1643432335,1323053555,1377906382,1608824934,21194,SRX9755044,SRS7944754,SRA1178968,southwest university|college of fisheries,southwest university,2,0.93408,0.93293,0.08519,0.08534,0.70952,0.71029,0.56062,0.52712,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-30,Undetermined,Undetermined,Kidney,Renal System 62703,SRR13327933,SRX9755043,SRS7944753,SRP299775,PRJNA688655,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,PRJNA688655,Other,,,,,WT 0h k3,WT 0h k3,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:8|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,WT 0h k3,WT k3,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299775,,,Mut_0h_k3.raw_1.fastq.gz Mut_0h_k3.raw_2.fastq.gz,fastq fastq,5891495100.0,19638317.0,Mut 0h k3.raw 1.fastq.gz,0:150 1:150,A:1626936306;C:1303639063;G:1370950045;T:1589948552;N:21134,150,150,,,1626936306,1303639063,1370950045,1589948552,21134,SRX9755043,SRS7944753,SRA1178968,southwest university|college of fisheries,southwest university,2,0.93117,0.93006,0.09742,0.09724,0.70396,0.7041,0.53898,0.53936,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-30,Undetermined,Undetermined,Kidney,Renal System 62704,SRR13327934,SRX9755042,SRS7944752,SRP299775,PRJNA688655,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,PRJNA688655,Other,,,,,WT 0h k2,WT 0h k2,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,WT 0h k2,WT k2,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299775,,,Mut_0h_k2.raw_1.fastq.gz Mut_0h_k2.raw_2.fastq.gz,fastq fastq,5543211000.0,18477370.0,Mut 0h k2.raw 1.fastq.gz,0:150 1:150,A:1531314014;C:1225009325;G:1291427420;T:1495440150;N:20091,150,150,,,1531314014,1225009325,1291427420,1495440150,20091,SRX9755042,SRS7944752,SRA1178968,southwest university|college of fisheries,southwest university,2,0.93219,0.93172,0.10466,0.10531,0.70141,0.70104,0.53257,0.53162,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-30,Undetermined,Undetermined,Kidney,Renal System 62705,SRR13327935,SRX9755041,SRS7944751,SRP299775,PRJNA688655,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,PRJNA688655,Other,,,,,WT 0h k1,WT 0h k1,,strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:6|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish,WT 0h k1,WT k1,other,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,HiSeq X Ten,,SRP299775,,,Mut_0h_k1.raw_1.fastq.gz Mut_0h_k1.raw_2.fastq.gz,fastq fastq,6954804600.0,23182682.0,Mut 0h k1.raw 1.fastq.gz,0:150 1:150,A:1929335607;C:1531367791;G:1605966700;T:1888112325;N:22177,150,150,,,1929335607,1531367791,1605966700,1888112325,22177,SRX9755041,SRS7944751,SRA1178968,southwest university|college of fisheries,southwest university,2,0.9401,0.93841,0.08725,0.08725,0.71536,0.71569,0.56381,0.56397,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,China,2020-12-30,Undetermined,Undetermined,Kidney,Renal System