rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
60,DRR032764,DRX029570,DRS049969,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr shield 2,SAMD00028161,,sample name:Dr shield 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:shield|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028161,DRX029570,Dr shield 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028161,,,,3644397900.0,36443979.0,DRR032764,0:100 1:0,A:986071173;C:842367218;G:837686080;T:978236607;N:36822,100,0,,,986071173,842367218,837686080,978236607,36822,DRX029570,DRS049969,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92419,,0.08269,,0.75558,,0.47863,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Gastrula,Embryo,Whole Organism,All anatomical structures
61,DRR032763,DRX029569,DRS049968,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr shield 1,SAMD00028160,,sample name:Dr shield 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:shield|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028160,DRX029569,Dr shield 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028160,,,,3834622000.0,38346220.0,DRR032763,0:100 1:0,A:1043352851;C:880011834;G:876775415;T:1034444253;N:37647,100,0,,,1043352851,880011834,876775415,1034444253,37647,DRX029569,DRS049968,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92305,,0.09126,,0.75481,,0.47587,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Gastrula,Embryo,Whole Organism,All anatomical structures
62,DRR032762,DRX029568,DRS049967,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime5 6 3,SAMD00028159,,sample name:Dr prime5 6 3|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime5 6|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028159,DRX029568,Dr prime5 6 3,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028159,,,,3903332800.0,39033328.0,DRR032762,0:100 1:0,A:1050045822;C:908538410;G:900588661;T:1044116537;N:43370,100,0,,,1050045822,908538410,900588661,1044116537,43370,DRX029568,DRS049967,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92761,,0.07976,,0.69126,,0.46568,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
63,DRR032761,DRX029567,DRS049966,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime5 6 2,SAMD00028158,,sample name:Dr prime5 6 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime5 6|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028158,DRX029567,Dr prime5 6 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028158,,,,3678549700.0,36785497.0,DRR032761,0:100 1:0,A:986526644;C:857762765;G:853417738;T:980801764;N:40789,100,0,,,986526644,857762765,853417738,980801764,40789,DRX029567,DRS049966,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92689,,0.07872,,0.6928,,0.46577,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
64,DRR032760,DRX029566,DRS049965,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime5 6 1,SAMD00028157,,sample name:Dr prime5 6 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime5 6|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028157,DRX029566,Dr prime5 6 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028157,,,,3863129500.0,38631295.0,DRR032760,0:100 1:0,A:1035240477;C:901625010;G:895370149;T:1030851937;N:41927,100,0,,,1035240477,901625010,895370149,1030851937,41927,DRX029566,DRS049965,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92337,,0.07522,,0.69315,,0.46516,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
65,DRR032759,DRX029565,DRS049964,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime25 2,SAMD00028156,,sample name:Dr prime25 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime25|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028156,DRX029565,Dr prime25 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028156,,,,3750136100.0,37501361.0,DRR032759,0:100 1:0,A:1013528040;C:866734984;G:862431819;T:1007403208;N:38049,100,0,,,1013528040,866734984,862431819,1007403208,38049,DRX029565,DRS049964,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92019,,0.09079,,0.68304,,0.47083,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
66,DRR032758,DRX029564,DRS049963,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime25 1,SAMD00028155,,sample name:Dr prime25 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime25|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028155,DRX029564,Dr prime25 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028155,,,,3544862700.0,35448627.0,DRR032758,0:100 1:0,A:952135895;C:825841753;G:821757889;T:945087927;N:39236,100,0,,,952135895,825841753,821757889,945087927,39236,DRX029564,DRS049963,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92229,,0.08344,,0.68525,,0.466,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
67,DRR032757,DRX029563,DRS049962,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 97 individuals,Dr bud 2,SAMD00028154,,sample name:Dr bud 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:bud|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028154,DRX029563,Dr bud 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028154,,,,4104778200.0,41047782.0,DRR032757,0:100 1:0,A:1116316188;C:944738800;G:936257056;T:1107423486;N:42670,100,0,,,1116316188,944738800,936257056,1107423486,42670,DRX029563,DRS049962,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92945,,0.10493,,0.73407,,0.47824,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
68,DRR032756,DRX029562,DRS049961,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr bud 1,SAMD00028153,,sample name:Dr bud 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:bud|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028153,DRX029562,Dr bud 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028153,,,,4540291000.0,45402910.0,DRR032756,0:100 1:0,A:1237914068;C:1042346110;G:1033172731;T:1226799791;N:58300,100,0,,,1237914068,1042346110,1033172731,1226799791,58300,DRX029562,DRS049961,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92628,,0.10478,,0.7391,,0.46461,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
69,DRR032755,DRX029561,DRS049960,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr 90epiboly 2,SAMD00028152,,sample name:Dr 90epiboly 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:90epiboly|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028152,DRX029561,Dr 90epiboly 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028152,,,,3572358600.0,35723586.0,DRR032755,0:100 1:0,A:971653450;C:821326559;G:816855636;T:962477457;N:45498,100,0,,,971653450,821326559,816855636,962477457,45498,DRX029561,DRS049960,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92485,,0.10642,,0.74213,,0.47012,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Gastrula,Embryo,Whole Organism,All anatomical structures
70,DRR032754,DRX029560,DRS049959,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr 90epiboly 1,SAMD00028151,,sample name:Dr 90epiboly 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:90epiboly|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028151,DRX029560,Dr 90epiboly 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028151,,,,3423980500.0,34239805.0,DRR032754,0:100 1:0,A:933088185;C:785251613;G:780911148;T:924686406;N:43148,100,0,,,933088185,785251613,780911148,924686406,43148,DRX029560,DRS049959,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92436,,0.10881,,0.74255,,0.47068,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Gastrula,Embryo,Whole Organism,All anatomical structures
71,DRR032753,DRX029559,DRS049958,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 114 individuals,Dr 8cell 2,SAMD00028150,,sample name:Dr 8cell 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:8cell|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028150,DRX029559,Dr 8cell 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028150,,,,3708921900.0,37089219.0,DRR032753,0:100 1:0,A:985502141;C:874161613;G:869551685;T:979663686;N:42775,100,0,,,985502141,874161613,869551685,979663686,42775,DRX029559,DRS049958,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.93329,,0.02366,,0.78896,,0.47447,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Cleavage,Embryo,Whole Organism,All anatomical structures
72,DRR032752,DRX029558,DRS049957,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 96 individuals,Dr 8cell 1,SAMD00028149,,sample name:Dr 8cell 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:8cell|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028149,DRX029558,Dr 8cell 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028149,,,,3666991200.0,36669912.0,DRR032752,0:100 1:0,A:976118513;C:862559696;G:858017821;T:970254302;N:40868,100,0,,,976118513,862559696,858017821,970254302,40868,DRX029558,DRS049957,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.934,,0.02403,,0.78877,,0.46902,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Cleavage,Embryo,Whole Organism,All anatomical structures
73,DRR032751,DRX029557,DRS049956,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr 75epiboly 2,SAMD00028148,,sample name:Dr 75epiboly 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:75epiboly|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028148,DRX029557,Dr 75epiboly 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028148,,,,3252021500.0,32520215.0,DRR032751,0:100 1:0,A:885527595;C:746750899;G:742907892;T:876794123;N:40991,100,0,,,885527595,746750899,742907892,876794123,40991,DRX029557,DRS049956,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92594,,0.10181,,0.74862,,0.47789,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Gastrula,Embryo,Whole Organism,All anatomical structures
74,DRR032750,DRX029556,DRS049955,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr 75epiboly 1,SAMD00028147,,sample name:Dr 75epiboly 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:75epiboly|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028147,DRX029556,Dr 75epiboly 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028147,,,,3785053700.0,37850537.0,DRR032750,0:100 1:0,A:1029014798;C:870946157;G:867537069;T:1017508684;N:46992,100,0,,,1029014798,870946157,867537069,1017508684,46992,DRX029556,DRS049955,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92346,,0.10046,,0.74921,,0.47295,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Gastrula,Embryo,Whole Organism,All anatomical structures
75,DRR032749,DRX029555,DRS049954,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 72h 2,SAMD00028146,,sample name:Dr 72h 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:72h Protruding mouth|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028146,DRX029555,Dr 72h 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028146,,,,3429795800.0,34297958.0,DRR032749,0:100 1:0,A:928062015;C:792470305;G:786930881;T:922296289;N:36310,100,0,,,928062015,792470305,786930881,922296289,36310,DRX029555,DRS049954,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.91821,,0.09774,,0.65437,,0.46443,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Larval,Larval,Whole Organism,All anatomical structures
76,DRR032748,DRX029554,DRS049953,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 72h 1,SAMD00028145,,sample name:Dr 72h 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:72h Protruding mouth|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028145,DRX029554,Dr 72h 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028145,,,,3897194500.0,38971945.0,DRR032748,0:100 1:0,A:1050989414;C:903225496;G:895783177;T:1047153493;N:42920,100,0,,,1050989414,903225496,895783177,1047153493,42920,DRX029554,DRS049953,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92211,,0.09393,,0.65486,,0.45971,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Larval,Larval,Whole Organism,All anatomical structures
77,DRR032747,DRX029553,DRS049952,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 6somite 2,SAMD00028144,,sample name:Dr 6somite 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:6somite|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028144,DRX029553,Dr 6somite 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028144,,,,3704431000.0,37044310.0,DRR032747,0:100 1:0,A:1001844161;C:856702913;G:850695568;T:995148798;N:39560,100,0,,,1001844161,856702913,850695568,995148798,39560,DRX029553,DRS049952,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92633,,0.09211,,0.72107,,0.47195,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Segmentation,Embryo,Whole Organism,All anatomical structures
78,DRR032746,DRX029552,DRS049951,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 6somite 1,SAMD00028143,,sample name:Dr 6somite 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:6somite|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028143,DRX029552,Dr 6somite 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028143,,,,3529311900.0,35293119.0,DRR032746,0:100 1:0,A:953957996;C:816824469;G:811403696;T:947089530;N:36209,100,0,,,953957996,816824469,811403696,947089530,36209,DRX029552,DRS049951,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92437,,0.09257,,0.72113,,0.47004,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Segmentation,Embryo,Whole Organism,All anatomical structures
79,DRR032745,DRX029551,DRS049950,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 60h 2,SAMD00028142,,sample name:Dr 60h 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:60h Pec fin|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028142,DRX029551,Dr 60h 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028142,,,,3875337000.0,38753370.0,DRR032745,0:100 1:0,A:1042558903;C:899892111;G:896867583;T:1035981420;N:36983,100,0,,,1042558903,899892111,896867583,1035981420,36983,DRX029551,DRS049950,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.91891,,0.09445,,0.66156,,0.45564,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Hatching,Embryo,Whole Organism,All anatomical structures
80,DRR032744,DRX029550,DRS049949,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 60h 1,SAMD00028141,,sample name:Dr 60h 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:60h Pec fin|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028141,DRX029550,Dr 60h 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028141,,,,3538468200.0,35384682.0,DRR032744,0:100 1:0,A:960664313;C:812459988;G:809014008;T:956295205;N:34686,100,0,,,960664313,812459988,809014008,956295205,34686,DRX029550,DRS049949,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.91388,,0.10346,,0.66076,,0.45203,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Hatching,Embryo,Whole Organism,All anatomical structures
81,DRR032743,DRX029549,DRS049948,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 5day 3,SAMD00028140,,sample name:Dr 5day 3|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:5day|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028140,DRX029549,Dr 5day 3,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028140,,,,3884716000.0,38847160.0,DRR032743,0:100 1:0,A:1040550584;C:905663425;G:904247323;T:1034215019;N:39649,100,0,,,1040550584,905663425,904247323,1034215019,39649,DRX029549,DRS049948,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92219,,0.08287,,0.65863,,0.47377,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Larval,Larval,Whole Organism,All anatomical structures
82,DRR032742,DRX029548,DRS049947,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 5day 2,SAMD00028139,,sample name:Dr 5day 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:5day|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028139,DRX029548,Dr 5day 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028139,,,,3893708700.0,38937087.0,DRR032742,0:100 1:0,A:1050850168;C:899863467;G:897224776;T:1045729184;N:41105,100,0,,,1050850168,899863467,897224776,1045729184,41105,DRX029548,DRS049947,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.91671,,0.0991,,0.65161,,0.47454,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Larval,Larval,Whole Organism,All anatomical structures
83,DRR032741,DRX029547,DRS049946,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 5day 1,SAMD00028138,,sample name:Dr 5day 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:5day|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028138,DRX029547,Dr 5day 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028138,,,,3807570600.0,38075706.0,DRR032741,0:100 1:0,A:1022590228;C:884655401;G:882546091;T:1017737883;N:40997,100,0,,,1022590228,884655401,882546091,1017737883,40997,DRX029547,DRS049946,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.9182,,0.09442,,0.65525,,0.46661,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Larval,Larval,Whole Organism,All anatomical structures
84,DRR032740,DRX029546,DRS049945,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 48h 2,SAMD00028137,,sample name:Dr 48h 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:48h Long pec|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028137,DRX029546,Dr 48h 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028137,,,,3702804700.0,37028047.0,DRR032740,0:100 1:0,A:993931475;C:862403562;G:857808891;T:988623734;N:37038,100,0,,,993931475,862403562,857808891,988623734,37038,DRX029546,DRS049945,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92508,,0.08526,,0.68349,,0.45769,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Hatching,Embryo,Whole Organism,All anatomical structures
85,DRR032739,DRX029545,DRS049944,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr 48h 1,SAMD00028136,,sample name:Dr 48h 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:48h Long pec|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028136,DRX029545,Dr 48h 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028136,,,,3980240400.0,39802404.0,DRR032739,0:100 1:0,A:1070497788;C:925240883;G:920038728;T:1064422474;N:40527,100,0,,,1070497788,925240883,920038728,1064422474,40527,DRX029545,DRS049944,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92349,,0.08681,,0.67874,,0.46565,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Hatching,Embryo,Whole Organism,All anatomical structures
86,DRR032738,DRX029544,DRS049943,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr 32cell 2,SAMD00028135,,sample name:Dr 32cell 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:32cell|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028135,DRX029544,Dr 32cell 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028135,,,,3678713000.0,36787130.0,DRR032738,0:100 1:0,A:981005900;C:863203049;G:859660640;T:974807835;N:35576,100,0,,,981005900,863203049,859660640,974807835,35576,DRX029544,DRS049943,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.93302,,0.02468,,0.77441,,0.47485,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Cleavage,Embryo,Whole Organism,All anatomical structures
87,DRR032737,DRX029543,DRS049942,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 95 individuals,Dr 32cell 1,SAMD00028134,,sample name:Dr 32cell 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:32cell|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028134,DRX029543,Dr 32cell 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028134,,,,3870906500.0,38709065.0,DRR032737,0:100 1:0,A:1030407751;C:909948718;G:905608620;T:1024897443;N:43968,100,0,,,1030407751,909948718,905608620,1024897443,43968,DRX029543,DRS049942,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.93364,,0.02484,,0.77307,,0.47588,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Cleavage,Embryo,Whole Organism,All anatomical structures
88,DRR032736,DRX029542,DRS049941,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr zfs:0000015 2,SAMD00028133,,sample name:Dr zfs:0000015 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:zfs:0000015|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028133,DRX029542,Dr zfs:0000015 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028133,,,,3129028500.0,31290285.0,DRR032736,0:100 1:0,A:849515903;C:721550282;G:717777586;T:840154982;N:29747,100,0,,,849515903,721550282,717777586,840154982,29747,DRX029542,DRS049941,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92724,,0.07971,,0.74657,,0.47796,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Blastula,Embryo,Whole Organism,All anatomical structures
89,DRR032735,DRX029541,DRS049940,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr zfs:0000015 1,SAMD00028132,,sample name:Dr zfs:0000015 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:zfs:0000015|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028132,DRX029541,Dr zfs:0000015 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028132,,,,4310219700.0,43102197.0,DRR032735,0:100 1:0,A:1169701983;C:993241399;G:986263558;T:1160969083;N:43677,100,0,,,1169701983,993241399,986263558,1160969083,43677,DRX029541,DRS049940,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92609,,0.07773,,0.74349,,0.47849,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Blastula,Embryo,Whole Organism,All anatomical structures
90,DRR032734,DRX029540,DRS049939,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 107 individuals,Dr 2cell 2,SAMD00028131,,sample name:Dr 2cell 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:2cell|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028131,DRX029540,Dr 2cell 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028131,,,,3687517000.0,36875170.0,DRR032734,0:100 1:0,A:975272080;C:873518282;G:869743434;T:968941851;N:41353,100,0,,,975272080,873518282,869743434,968941851,41353,DRX029540,DRS049939,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.93204,,0.02088,,0.81639,,0.47553,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Cleavage,Embryo,Whole Organism,All anatomical structures
91,DRR032733,DRX029539,DRS049938,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 108 individuals,Dr 2cell 1,SAMD00028130,,sample name:Dr 2cell 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:2cell|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028130,DRX029539,Dr 2cell 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028130,,,,4156651100.0,41566511.0,DRR032733,0:100 1:0,A:1099943617;C:985498415;G:978884426;T:1092278665;N:45977,100,0,,,1099943617,985498415,978884426,1092278665,45977,DRX029539,DRS049938,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.93452,,0.02198,,0.81197,,0.47342,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Cleavage,Embryo,Whole Organism,All anatomical structures
92,DRR032732,DRX029538,DRS049937,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 80 individuals,Dr 14somite 3,SAMD00028129,,sample name:Dr 14somite 3|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:14somite|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028129,DRX029538,Dr 14somite 3,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028129,,,,3734610500.0,37346105.0,DRR032732,0:100 1:0,A:1009418541;C:863710067;G:858061383;T:1003378800;N:41709,100,0,,,1009418541,863710067,858061383,1003378800,41709,DRX029538,DRS049937,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92401,,0.08815,,0.70816,,0.46602,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Segmentation,Embryo,Whole Organism,All anatomical structures
93,DRR032731,DRX029537,DRS049936,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 80 individuals,Dr 14somite 2,SAMD00028128,,sample name:Dr 14somite 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:14somite|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028128,DRX029537,Dr 14somite 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028128,,,,3715174200.0,37151742.0,DRR032731,0:100 1:0,A:1000703508;C:862290629;G:858173468;T:993968396;N:38199,100,0,,,1000703508,862290629,858173468,993968396,38199,DRX029537,DRS049936,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92491,,0.0819,,0.71068,,0.46957,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Segmentation,Embryo,Whole Organism,All anatomical structures
94,DRR032730,DRX029536,DRS049935,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief  taking advantages of Illumina sequencing  RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 80 individuals,Dr 14somite 1,SAMD00028127,,sample name:Dr 14somite 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:14somite|genotype:wild type|phenotype:wild type|sex:male  female  and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028127,DRX029536,Dr 14somite 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>100</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028127,,,,3744386000.0,37443860.0,DRR032730,0:100 1:0,A:1014537326;C:864070910;G:859190201;T:1006549502;N:38061,100,0,,,1014537326,864070910,859190201,1006549502,38061,DRX029536,DRS049935,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92378,,0.08957,,0.7068,,0.47493,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Segmentation,Embryo,Whole Organism,All anatomical structures
7946,ERR015563,ERX005934,ERS012707,ERP000263,PRJEB2208,Zebrafish gene three prime end pull down for genome annotation,E-MTAB-308,Transcriptome Analysis,,,,,E MTAB 308:Zebrafish embryo 2 dpf 2,SAMEA898403,Wellcome Sanger Institute,Age:2 days|Alias:E MTAB 308:Zebrafish embryo 2 dpf 2|Broker name:ArrayExpress|Description:Protocols: Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.|DevelopmentalStage:embryo|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 08 19T15:57:35Z|INSDC last update:2018 03 08T15:25:04Z|INSDC status:public|InitialTimePoint:fertilization|OrganismPart:whole organism|SRA accession:ERS012707|Sample Name:ERS012707|Sex:unknown sex|StrainOrLine:Tuebingen|Title:Danio rerio,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation,E MTAB 308:Illumina Genome Analyzer II sequencing of Zebrafish embryo 2 dpf three prime pull down paired end 250 to 300 bp insert,Zebrafish embro 2 dpf mRNA three prime end,Zebrafish gene three prime end pull down for genome annotation,20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl.   RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin  magnetic beads.   post washing by pull down on a magnet  the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB.   post further washing  the double strand cDNA was released from the beads with BpmI NEB.   The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.,Experimental Factor: AGE:2 d|Experimental Factor: DEVELOPMENTAL STAGE:embryo|Experimental Factor: ORGANISM PART:whole organism|Experimental Factor: SEX:unknown sex,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000263,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation,ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16,3444_2.srf,srf,990308120.0,6515185.0,E MTAB 308:Illumina Genome Analyzer II sequencing of Zebrafish embryo 2 dpf three prime pull down paired end 250 to 300 bp insert,0:76 1:76,A:279665076;C:200433201;G:189692111;T:304366697;N:16151035,76,76,,,279665076,200433201,189692111,304366697,16151035,ERX005934,ERS012707,ERA010603,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.93964,0.94008,0.40095,0.39969,0.74424,0.74915,0.49535,0.49761,76,76,B,B,biological fallback assumption,illumina,early_illumina,3prime,other,unknown,bulk,unknown,unknown,,United Kingdom,2010-08-19,Hatching,Embryo,Whole Organism,All anatomical structures
7947,ERR015564,ERX005933,ERS012706,ERP000263,PRJEB2208,Zebrafish gene three prime end pull down for genome annotation,E-MTAB-308,Transcriptome Analysis,,,,,E MTAB 308:Zebrafish embryo 3 dpf 2,SAMEA898404,Wellcome Sanger Institute,Age:3 days|Alias:E MTAB 308:Zebrafish embryo 3 dpf 2|Broker name:ArrayExpress|Description:Protocols: Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.|DevelopmentalStage:embryo|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 08 19T15:57:35Z|INSDC last update:2018 03 08T15:25:04Z|INSDC status:public|InitialTimePoint:fertilization|OrganismPart:whole organism|SRA accession:ERS012706|Sample Name:ERS012706|Sex:unknown sex|StrainOrLine:Tuebingen|Title:Danio rerio,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation,E MTAB 308:Illumina Genome Analyzer II sequencing of Zebrafish embryo 3 dpf three prime pull down paired end 250 to 300 bp insert,Zebrafish embro 3 dpf mRNA three prime end,Zebrafish gene three prime end pull down for genome annotation,20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl.   RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin  magnetic beads.   post washing by pull down on a magnet  the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB.   post further washing  the double strand cDNA was released from the beads with BpmI NEB.   The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.,Experimental Factor: AGE:3 d|Experimental Factor: DEVELOPMENTAL STAGE:embryo|Experimental Factor: ORGANISM PART:whole organism|Experimental Factor: SEX:unknown sex,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000263,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation,ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16,3444_3.srf,srf,1572215648.0,10343524.0,E MTAB 308:Illumina Genome Analyzer II sequencing of Zebrafish embryo 3 dpf three prime pull down paired end 250 to 300 bp insert,0:76 1:76,A:395189954;C:374281517;G:356666372;T:420372888;N:25704917,76,76,,,395189954,374281517,356666372,420372888,25704917,ERX005933,ERS012706,ERA010603,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.96337,0.96626,0.12558,0.12974,0.7824,0.79086,0.40731,0.41802,76,76,B,B,biological fallback assumption,illumina,early_illumina,3prime,other,unknown,bulk,unknown,unknown,,United Kingdom,2010-08-19,Larval,Larval,Whole Organism,All anatomical structures
7948,ERR015562,ERX005932,ERS012705,ERP000263,PRJEB2208,Zebrafish gene three prime end pull down for genome annotation,E-MTAB-308,Transcriptome Analysis,,,,,E MTAB 308:Zebrafish embryo 1 dpf 2,SAMEA898401,Wellcome Sanger Institute,Age:1 days|Alias:E MTAB 308:Zebrafish embryo 1 dpf 2|Broker name:ArrayExpress|Description:Protocols: Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.|DevelopmentalStage:embryo|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 08 19T15:57:35Z|INSDC last update:2018 03 08T15:25:04Z|INSDC status:public|InitialTimePoint:fertilization|OrganismPart:whole organism|SRA accession:ERS012705|Sample Name:ERS012705|Sex:unknown sex|StrainOrLine:Tuebingen|Title:Danio rerio,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation,E MTAB 308:Illumina Genome Analyzer II sequencing of Zebrafish embryo 1 dpf three prime pull down paired end 250 to 300 bp insert,Zebrafish embro 1 dpf mRNA three prime end,Zebrafish gene three prime end pull down for genome annotation,20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl.   RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin  magnetic beads.   post washing by pull down on a magnet  the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB.   post further washing  the double strand cDNA was released from the beads with BpmI NEB.   The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.,Experimental Factor: AGE:1 d|Experimental Factor: DEVELOPMENTAL STAGE:embryo|Experimental Factor: ORGANISM PART:whole organism|Experimental Factor: SEX:unknown sex,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000263,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation,ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16,3444_1.srf,srf,1358041568.0,8934484.0,E MTAB 308:Illumina Genome Analyzer II sequencing of Zebrafish embryo 1 dpf three prime pull down paired end 250 to 300 bp insert,0:76 1:76,A:376045982;C:281568469;G:271365867;T:407279179;N:21782071,76,76,,,376045982,281568469,271365867,407279179,21782071,ERX005932,ERS012705,ERA010603,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.94607,0.94542,0.3002,0.30139,0.73584,0.74038,0.51058,0.51233,76,76,B,B,biological fallback assumption,illumina,early_illumina,3prime,other,unknown,bulk,unknown,unknown,,United Kingdom,2010-08-19,Pharyngula,Embryo,Whole Organism,All anatomical structures
7949,ERR015566,ERX005931,ERS000090,ERP000263,PRJEB2208,Zebrafish gene three prime end pull down for genome annotation,E-MTAB-308,Transcriptome Analysis,,,,,ZF ovary sample1,SAMEA708832,Wellcome Sanger Institute,Alias:ZF ovary sample1|Description:RNA extracted from adult zebrafish ovary|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:24:37Z|INSDC status:public|SRA accession:ERS000090|Sample Name:ERS000090|Sex:female|Strain:Tuebingen|Title:Danio rerio,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation,E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish ovary dpf three prime pull down paired end 250 to 300 bp insert,Zebrafish adult ovary mRNA three prime end,Zebrafish gene three prime end pull down for genome annotation,20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl.   RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin  magnetic beads.   post washing by pull down on a magnet  the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB.   post further washing  the double strand cDNA was released from the beads with BpmI NEB.   The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.,Experimental Factor: DEVELOPMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:ovary|Experimental Factor: SEX:female,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000263,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation,ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16,3444_6.srf,srf,1150193272.0,7567061.0,E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish ovary dpf three prime pull down paired end 250 to 300 bp insert,0:76 1:76,A:280621121;C:291623460;G:285422205;T:273609496;N:18916990,76,76,,,280621121,291623460,285422205,273609496,18916990,ERX005931,ERS000090,ERA010603,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.96663,0.96605,0.0126,0.01249,0.82272,0.82548,0.45602,0.45286,76,76,B,B,biological fallback assumption,illumina,early_illumina,3prime,other,unknown,bulk,unknown,unknown,,United Kingdom,2010-02-26,Adult,Adult,Gonad,Reproductive System
7950,ERR015567,ERX005930,ERS000089,ERP000263,PRJEB2208,Zebrafish gene three prime end pull down for genome annotation,E-MTAB-308,Transcriptome Analysis,,,,RNA extracted from male adult zebrafish head,,SAMEA708835,SC,ENA FIRST PUBLIC:2010 02 26T10:44:13Z|ENA LAST UPDATE:2018 03 08T15:51:30Z|External Id:SAMEA708835|INSDC center name:SC|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:51:30Z|INSDC status:public|Submitter Id:ZF male head sample1|common name:zebrafish|sample name:ZF male head sample1|scientific name:Danio rerio|sex:male|strain:Tuebingen,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation,E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish male head dpf three prime pull down paired end 250 to 300 bp insert,Zebrafish adult male head mRNA three prime end,Zebrafish gene three prime end pull down for genome annotation,20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl.   RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin  magnetic beads.   post washing by pull down on a magnet  the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB.   post further washing  the double strand cDNA was released from the beads with BpmI NEB.   The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.,Experimental Factor: DEVELOPMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:head|Experimental Factor: SEX:male,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000263,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation,ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16,3444_7.srf,srf,1384012896.0,9105348.0,E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish male head dpf three prime pull down paired end 250 to 300 bp insert,0:76 1:76,A:369912216;C:306963547;G:292249178;T:392053840;N:22834115,76,76,,,369912216,306963547,292249178,392053840,22834115,ERX005930,ERS000089,ERA010603,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.95932,0.96084,0.20719,0.21321,0.73959,0.74763,0.47227,0.48033,76,76,B,B,biological fallback assumption,illumina,early_illumina,3prime,other,unknown,bulk,unknown,unknown,,United Kingdom,2010-02-26,Adult,Adult,Head,Nervous System
7951,ERR015565,ERX005929,ERS012704,ERP000263,PRJEB2208,Zebrafish gene three prime end pull down for genome annotation,E-MTAB-308,Transcriptome Analysis,,,,,E MTAB 308:Zebrafish embryo 5 dpf 2,SAMEA980815,SC,Age:5 days|Alias:E MTAB 308:Zebrafish embryo 5 dpf 2|Broker name:ArrayExpress|Description:Protocols: Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.|DevelopmentalStage:embryo|INSDC center name:SC|INSDC first public:2010 08 19T15:57:35Z|INSDC last update:2018 03 08T15:25:04Z|INSDC status:public|InitialTimePoint:fertilization|OrganismPart:whole organism|SRA accession:ERS012704|Sample Name:ERS012704|Sex:unknown sex|StrainOrLine:Tuebingen|Title:Danio rerio,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation,E MTAB 308:Illumina Genome Analyzer II sequencing of Zebrafish embryo 5 dpf three prime pull down paired end 250 to 300 bp insert,Zebrafish embro 5 dpf mRNA three prime end,Zebrafish gene three prime end pull down for genome annotation,20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl.   RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin  magnetic beads.   post washing by pull down on a magnet  the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB.   post further washing  the double strand cDNA was released from the beads with BpmI NEB.   The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.,Experimental Factor: AGE:5 d|Experimental Factor: DEVELOPMENTAL STAGE:embryo|Experimental Factor: ORGANISM PART:whole organism|Experimental Factor: SEX:unknown sex,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000263,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation,ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16,3444_5.srf,srf,1399629832.0,9208091.0,E MTAB 308:Illumina Genome Analyzer II sequencing of Zebrafish embryo 5 dpf three prime pull down paired end 250 to 300 bp insert,0:76 1:76,A:389123080;C:293319124;G:279736391;T:414876632;N:22574605,76,76,,,389123080,293319124,279736391,414876632,22574605,ERX005929,ERS012704,ERA010603,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.92999,0.9332,0.45309,0.46162,0.72419,0.73919,0.49892,0.50512,76,76,B,B,biological fallback assumption,illumina,early_illumina,3prime,other,unknown,bulk,unknown,unknown,,United Kingdom,2010-08-19,Larval,Larval,Whole Organism,All anatomical structures
7952,ERR015568,ERX005928,ERS000087,ERP000263,PRJEB2208,Zebrafish gene three prime end pull down for genome annotation,E-MTAB-308,Transcriptome Analysis,,,,,ZF male sample1,SAMEA708829,Wellcome Sanger Institute,Alias:ZF male sample1|Description:RNA extracted from whole male adult zebrafish without xxx|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:24:37Z|INSDC status:public|SRA accession:ERS000087|Sample Name:ERS000087|Sex:male|Strain:Tuebingen|Title:Danio rerio,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation,E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish male body dpf three prime pull down paired end 250 to 300 bp insert,Zebrafish adult male body mRNA three prime end,Zebrafish gene three prime end pull down for genome annotation,20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl.   RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin  magnetic beads.   post washing by pull down on a magnet  the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB.   post further washing  the double strand cDNA was released from the beads with BpmI NEB.   The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.,Experimental Factor: DEVELOPMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:whole fish without xxx|Experimental Factor: SEX:male,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000263,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation,ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16,3444_8.srf,srf,1012667320.0,6662285.0,E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish male body dpf three prime pull down paired end 250 to 300 bp insert,0:76 1:76,A:262408681;C:234183204;G:228254982;T:271015871;N:16804582,76,76,,,262408681,234183204,228254982,271015871,16804582,ERX005928,ERS000087,ERA010603,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.95123,0.96116,0.23163,0.22276,0.77847,0.78648,0.42301,0.43528,76,76,B,B,biological fallback assumption,illumina,early_illumina,3prime,other,unknown,bulk,unknown,unknown,,United Kingdom,2010-02-26,Adult,Adult,Whole Organism,All anatomical structures
9337,ERR2865439,ERX2871399,ERS2871019,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development  we identified prominent coloboma and cell autonomous retinal neuron differentiation  lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present  most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp  which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when  and locations where  post mitotic cells have differentiated in wild type siblings. Indeed  RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated  for instance  in axon guidance  that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise  or promote transitions in  cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,sibling 3,SAMEA5059848,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059848|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele sibling3|common name:zebrafish|sample name:ele sibling3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 6,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_sib_F_CTTGTA_L004_R2_001.fastq.gz ele_sib_F_CTTGTA_L004_R1_001.fastq.gz,fastq fastq,2823621200.0,14118106.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 6,0:100 1:100,A:752781583;C:663838191;G:656422705;T:750213027;N:365694,100,100,,,752781583,663838191,656422705,750213027,365694,ERX2871399,ERS2871019,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.95595,0.95486,0.09407,0.09431,0.67529,0.67673,0.45173,0.44515,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise
9338,ERR2865438,ERX2871398,ERS2871018,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development  we identified prominent coloboma and cell autonomous retinal neuron differentiation  lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present  most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp  which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when  and locations where  post mitotic cells have differentiated in wild type siblings. Indeed  RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated  for instance  in axon guidance  that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise  or promote transitions in  cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,sibling 2,SAMEA5059847,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059847|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele sibling2|common name:zebrafish|sample name:ele sibling2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 5,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_sib_D_GCCAAT_L004_R1_001.fastq.gz ele_sib_D_GCCAAT_L004_R2_001.fastq.gz,fastq fastq,4217962600.0,21089813.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 5,0:100 1:100,A:1119324653;C:996982862;G:984906708;T:1116209552;N:538825,100,100,,,1119324653,996982862,984906708,1116209552,538825,ERX2871398,ERS2871018,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.95341,0.95274,0.09215,0.09238,0.67296,0.67493,0.46185,0.4648,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise
9339,ERR2865437,ERX2871397,ERS2871017,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development  we identified prominent coloboma and cell autonomous retinal neuron differentiation  lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present  most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp  which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when  and locations where  post mitotic cells have differentiated in wild type siblings. Indeed  RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated  for instance  in axon guidance  that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise  or promote transitions in  cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,sibling 1,SAMEA5059846,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059846|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele sibling1|common name:zebrafish|sample name:ele sibling1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 4,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_sib_B_TGACCA_L004_R1_001.fastq.gz ele_sib_B_TGACCA_L004_R2_001.fastq.gz,fastq fastq,5241628000.0,26208140.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 4,0:100 1:100,A:1393802799;C:1235804455;G:1219328189;T:1392021956;N:670601,100,100,,,1393802799,1235804455,1219328189,1392021956,670601,ERX2871397,ERS2871017,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.95296,0.95133,0.10275,0.1028,0.67018,0.67146,0.46488,0.46482,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise
9340,ERR2865436,ERX2871396,ERS2871016,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development  we identified prominent coloboma and cell autonomous retinal neuron differentiation  lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present  most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp  which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when  and locations where  post mitotic cells have differentiated in wild type siblings. Indeed  RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated  for instance  in axon guidance  that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise  or promote transitions in  cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,mutant3,SAMEA5059845,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059845|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele mutant3|common name:zebrafish|sample name:ele mutant3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 3,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_E_CAGATC_L004_R1_001.fastq.gz ele_E_CAGATC_L004_R2_001.fastq.gz,fastq fastq,3529752000.0,17648760.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 3,0:100 1:100,A:933354224;C:837396695;G:828677816;T:929860782;N:462483,100,100,,,933354224,837396695,828677816,929860782,462483,ERX2871396,ERS2871016,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.95621,0.95302,0.08584,0.08536,0.67048,0.67146,0.46615,0.46682,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise
9341,ERR2865435,ERX2871395,ERS2871015,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development  we identified prominent coloboma and cell autonomous retinal neuron differentiation  lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present  most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp  which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when  and locations where  post mitotic cells have differentiated in wild type siblings. Indeed  RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated  for instance  in axon guidance  that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise  or promote transitions in  cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,mutant2,SAMEA5059844,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059844|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele mutant2|common name:zebrafish|sample name:ele mutant2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 2,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_C_ACAGTG_L004_R1_001.fastq.gz ele_C_ACAGTG_L004_R2_001.fastq.gz,fastq fastq,3119723800.0,15598619.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 2,0:100 1:100,A:828335602;C:736691663;G:727853394;T:826449133;N:394008,100,100,,,828335602,736691663,727853394,826449133,394008,ERX2871395,ERS2871015,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.94967,0.94864,0.0992,0.09955,0.65928,0.66014,0.47042,0.46835,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise
9342,ERR2865434,ERX2871394,ERS2871014,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development  we identified prominent coloboma and cell autonomous retinal neuron differentiation  lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present  most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp  which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when  and locations where  post mitotic cells have differentiated in wild type siblings. Indeed  RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated  for instance  in axon guidance  that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise  or promote transitions in  cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,mutant1,SAMEA5059843,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059843|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele mutant1|common name:zebrafish|sample name:ele mutant1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:716 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_A_CGATGT_L004_R1_001.fastq.gz ele_A_CGATGT_L004_R2_001.fastq.gz,fastq fastq,2181939600.0,10909698.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 1,0:100 1:100,A:578028200;C:516249107;G:510965740;T:576417363;N:279190,100,100,,,578028200,516249107,510965740,576417363,279190,ERX2871394,ERS2871014,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.94968,0.94939,0.1131,0.11293,0.66245,0.66251,0.46654,0.47475,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise
9719,ERR3842000,ERX3854562,ERS4268611,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,Shield 4Ei,SAMEA6504165,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:05:06Z|ENA LAST UPDATE:2020 01 27T16:04:35Z|External Id:SAMEA6504165|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:05:06Z|INSDC last update:2020 01 27T16:04:35Z|INSDC status:public|Submitter Id:Shield 4Ei|common name:zebrafish|sample name:Shield 4Ei|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 10,Shield 4Ei,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1435748376.0,18891426.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 10,0:76,A:489056518;C:372290023;G:393663734;T:180723629;N:14472,76,,,,489056518,372290023,393663734,180723629,14472,ERX3854562,ERS4268611,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.11942,,0.03394,,0.98971,,0.62271,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Gastrula,Embryo,Undetermined,Embryo Imprecise
9720,ERR3841999,ERX3854561,ERS3556006,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,Shield 3,SAMEA5752547,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752547|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:10|common name:zebrafish|dev stage:Shield|sample name:10|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 9,Shield 3,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1168482976.0,15374776.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 9,0:76,A:516070340;C:238363428;G:268806793;T:145231087;N:11328,76,,,,516070340,238363428,268806793,145231087,11328,ERX3854561,ERS3556006,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.22586,,0.10275,,0.97281,,0.47683,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Gastrula,Embryo,Undetermined,Embryo Imprecise
9721,ERR3841998,ERX3854560,ERS3556007,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,Shield 4150NT,SAMEA5752548,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752548|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:11|common name:zebrafish|dev stage:Shield|sample name:11|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 8,Shield 150NT,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1188343980.0,15636105.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 8,0:76,A:580658556;C:223116826;G:260847322;T:123709681;N:11595,76,,,,580658556,223116826,260847322,123709681,11595,ERX3854560,ERS3556007,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.27037,,0.13972,,0.97392,,0.39459,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Gastrula,Embryo,Undetermined,Embryo Imprecise
9722,ERR3841997,ERX3854559,ERS3556004,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,Shield 1,SAMEA5752545,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752545|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:8|common name:zebrafish|dev stage:Shield|sample name:8|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 7,Shield 1,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1278891444.0,16827519.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 7,0:76,A:571738369;C:256385478;G:295145281;T:155610082;N:12234,76,,,,571738369,256385478,295145281,155610082,12234,ERX3854559,ERS3556004,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.23116,,0.11137,,0.97932,,0.45978,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Gastrula,Embryo,Undetermined,Embryo Imprecise
9723,ERR3841996,ERX3854558,ERS3556001,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,Sphere 3,SAMEA5752542,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752542|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:5|common name:zebrafish|dev stage:Sphere|sample name:5|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 6,Sphere 3,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1439954368.0,18946768.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 6,0:76,A:669362698;C:280496524;G:316727778;T:173353505;N:13863,76,,,,669362698,280496524,316727778,173353505,13863,ERX3854558,ERS3556001,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.26155,,0.12068,,0.96915,,0.45719,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Blastula,Embryo,Undetermined,Embryo Imprecise
9724,ERR3841995,ERX3854557,ERS3556000,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,Sphere 2,SAMEA5752541,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752541|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:4|common name:zebrafish|dev stage:Sphere|sample name:4|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 5,Sphere 2,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1592272200.0,20950950.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 5,0:76,A:757507948;C:308394094;G:342142775;T:184211881;N:15502,76,,,,757507948,308394094,342142775,184211881,15502,ERX3854557,ERS3556000,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.22483,,0.10923,,0.97646,,0.49458,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Blastula,Embryo,Undetermined,Embryo Imprecise
9725,ERR3841994,ERX3854556,ERS3555999,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,Sphere 1,SAMEA5752540,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752540|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:3|common name:zebrafish|dev stage:Sphere|sample name:3|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 4,Sphere 1,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1332363676.0,17531101.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 4,0:76,A:529354355;C:299445923;G:318745973;T:184804260;N:13165,76,,,,529354355,299445923,318745973,184804260,13165,ERX3854556,ERS3555999,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.21197,,0.0829,,0.98196,,0.55753,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Blastula,Embryo,Undetermined,Embryo Imprecise
9726,ERR3841993,ERX3854555,ERS3555998,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,64 cell 2,SAMEA5752539,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752539|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:2|common name:zebrafish|dev stage:64 cell|sample name:2|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 3,64 cell 3,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1579307816.0,20780366.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 3,0:76,A:589876602;C:380048242;G:392473318;T:216893826;N:15828,76,,,,589876602,380048242,392473318,216893826,15828,ERX3854555,ERS3555998,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.15411,,0.04564,,0.97883,,0.55376,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Cleavage,Embryo,Undetermined,Embryo Imprecise
9727,ERR3841992,ERX3854554,ERS3556003,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,64 cell 4Ei 10,SAMEA5752544,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752544|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:7|common name:zebrafish|dev stage:64 cell|sample name:7|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:406 2,64 cell 4Ei,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1213585480.0,15968230.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 2,0:76,A:548456563;C:244465528;G:271963808;T:148687764;N:11817,76,,,,548456563,244465528,271963808,148687764,11817,ERX3854554,ERS3556003,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.21973,,0.10926,,0.97419,,0.44348,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Cleavage,Embryo,Undetermined,Embryo Imprecise
9728,ERR3841991,ERX3854553,ERS3555997,ERP116106,PRJEB33323,Deconstructing the individual steps of vertebrate translation initiation,ena-STUDY-Computational Biology Unit-03-07-2019-10:11:34:314-422,Other,In eukaryotes  the number of ribosomes synthesizing a given protein depends on how many are recruited to its mRNA  their success in navigating its five prime untranslated region UTR and whether they recognize its start codon. Initiation of translation is a rate limiting step in protein synthesis and key to gene expression control 1  but despite this centrality it remains poorly understood 2 3. Here we introduce ribosome complex profiling RCP seq to capture the transcriptome wide occupancy of scanning  initiating  elongating and terminating ribosome complexes in a higher eukaryote. We track scanning and elongating ribosomes across all five prime UTRs in zebrafish  which enable us to assess the individual regulatory contributions from the three stages of initiation: ribosome recruitment  scanning of the five prime UTR and recognition of the start codon. Our data sheds light on small subunit recruitment to mRNAs presenting evidence for the threading model and demonstrates that sequence features regulate this recruitment. We estimate the processivity of scanning ribosomes as they traverse the five prime UTR and show that the repressive effects of upstream open reading frames depend on the efficiency of both translation initiation and termination. Finally  we determine the optimal initiation contexts by directly estimating the conversion of scanning to elongating ribosomes and demonstrate specific regulation of translation initiation at the endoplasmic reticulum. Our results open for the possibility of deconvoluting translation initiation into separate stages and provides the first view of global occupancy of ribosomal small subunits in a vertebrate.,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2019 07 03,,,64 cell 1,SAMEA5752538,Computational Biology Unit,ENA FIRST PUBLIC:2020 03 27T17:04:59Z|ENA LAST UPDATE:2019 07 03T10:01:14Z|External Id:SAMEA5752538|INSDC center name:Computational Biology Unit|INSDC first public:2020 03 27T17:04:59Z|INSDC last update:2019 07 03T10:01:14Z|INSDC status:public|Submitter Id:1|common name:zebrafish|dev stage:64 cell|sample name:1|scientific name:Danio rerio,,,,,,,,,NextSeq 500 sequencing,ena EXPERIMENT Computational Biology Unit 27 01 2020 16:24:36:405 1,64 cell 1,OTHER,RNA Seq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,NextSeq 500,,ERP116106,NextSeq 500 sequencing,ENA FIRST PUBLIC:2020 03 27|ENA LAST UPDATE:2020 02 14,,,1494930944.0,19670144.0,ena RUN Computational Biology Unit 27 01 2020 16:24:36:406 1,0:76,A:543017836;C:366974203;G:367027373;T:217896401;N:15131,76,,,,543017836,366974203,367027373,217896401,15131,ERX3854553,ERS3555997,ERA2359305,Computational Biology Unit|European Nucleotide Archive,Computational Biology Unit,1,0.2544,,0.08957,,0.96568,,0.55681,,76,,B,,usable mapping rate,illumina,nextseq,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2019-07-03,Cleavage,Embryo,Undetermined,Embryo Imprecise
9927,ERR4321734,ERX4268592,ERS4808149,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni R 3,SAMEA7047515,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047515|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni R 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni R 3|sampling site:right cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni R 3 p,Ctrl uni R 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_R_3_1.txt Ctrl_uni_R_3_2.txt,fastq fastq,7001062560.0,43756641.0,E MTAB 9321:Ctrl uni R 3 ,0:80 1:80,A:1691997382;C:1785031961;G:1794429141;T:1728559618;N:1044458,80,80,,,1691997382,1785031961,1794429141,1728559618,1044458,ERX4268592,ERS4808149,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.93339,0.93586,0.30761,0.30942,0.77031,0.77285,0.6535,0.63203,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9928,ERR4321733,ERX4268591,ERS4808148,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni R 2,SAMEA7047514,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047514|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni R 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni R 2|sampling site:right cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni R 2 p,Ctrl uni R 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_R_2_1.txt Ctrl_uni_R_2_2.txt,fastq fastq,6922683200.0,43266770.0,E MTAB 9321:Ctrl uni R 2 ,0:80 1:80,A:1632897692;C:1799983634;G:1813073970;T:1675697152;N:1030752,80,80,,,1632897692,1799983634,1813073970,1675697152,1030752,ERX4268591,ERS4808148,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.9343,0.93678,0.30732,0.30767,0.77226,0.77496,0.67322,0.638,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9929,ERR4321732,ERX4268590,ERS4808147,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni R 1,SAMEA7047513,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047513|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni R 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni R 1|sampling site:right cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni R 1 p,Ctrl uni R 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_R_1_1.txt Ctrl_uni_R_1_2.txt,fastq fastq,7277767680.0,45486048.0,E MTAB 9321:Ctrl uni R 1 ,0:80 1:80,A:1716271935;C:1890805720;G:1910764817;T:1758823538;N:1101670,80,80,,,1716271935,1890805720,1910764817,1758823538,1101670,ERX4268590,ERS4808147,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.93132,0.93607,0.29495,0.29694,0.77916,0.78023,0.66985,0.69546,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9930,ERR4321731,ERX4268589,ERS4808146,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni L 3,SAMEA7047512,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047512|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni L 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni L 3|sampling site:left cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni L 3 p,Ctrl uni L 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_L_3_1.txt Ctrl_uni_L_3_2.txt,fastq fastq,6575179520.0,41094872.0,E MTAB 9321:Ctrl uni L 3 ,0:80 1:80,A:1482672127;C:1776068618;G:1790187841;T:1525260758;N:990176,80,80,,,1482672127,1776068618,1790187841,1525260758,990176,ERX4268589,ERS4808146,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.94459,0.94749,0.2565,0.25972,0.77816,0.77881,0.67376,0.6469,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9931,ERR4321730,ERX4268588,ERS4808145,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni L 2,SAMEA7047511,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047511|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni L 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni L 2|sampling site:left cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni L 2 p,Ctrl uni L 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_L_2_1.txt Ctrl_uni_L_2_2.txt,fastq fastq,7275818080.0,45473863.0,E MTAB 9321:Ctrl uni L 2 ,0:80 1:80,A:1706173762;C:1899925486;G:1918514584;T:1750098284;N:1105964,80,80,,,1706173762,1899925486,1918514584,1750098284,1105964,ERX4268588,ERS4808145,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.93448,0.93897,0.29227,0.29368,0.77299,0.77587,0.67483,0.69924,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9932,ERR4321729,ERX4268587,ERS4808144,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni L 1,SAMEA7047510,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047510|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni L 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni L 1|sampling site:left cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni L 1 p,Ctrl uni L 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_L_1_1.txt Ctrl_uni_L_1_2.txt,fastq fastq,6259547520.0,39122172.0,E MTAB 9321:Ctrl uni L 1 ,0:80 1:80,A:1504594075;C:1603950703;G:1614410199;T:1535635583;N:956960,80,80,,,1504594075,1603950703,1614410199,1535635583,956960,ERX4268587,ERS4808144,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.92994,0.93276,0.31313,0.31395,0.77195,0.77372,0.66568,0.6882,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9933,ERR4321728,ERX4268586,ERS4808143,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl uni 3,SAMEA7047509,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047509|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl uni 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl uni 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl uni 3 p,3dpl uni 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_uni_3_1.txt 3dpl_uni_3_2.txt,fastq fastq,7967977760.0,49799861.0,E MTAB 9321:3dpl uni 3 ,0:80 1:80,A:2260216672;C:1639328793;G:1824529883;T:2241676126;N:2226286,80,80,,,2260216672,1639328793,1824529883,2241676126,2226286,ERX4268586,ERS4808143,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.90526,0.90173,0.44801,0.44169,0.73827,0.74854,0.51874,0.52672,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9934,ERR4321727,ERX4268585,ERS4808142,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl uni 2,SAMEA7047508,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047508|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl uni 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl uni 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl uni 2 p,3dpl uni 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_uni_2_1.txt 3dpl_uni_2_2.txt,fastq fastq,9215409600.0,57596310.0,E MTAB 9321:3dpl uni 2 ,0:80 1:80,A:2620502481;C:1914345031;G:2080364714;T:2597606056;N:2591318,80,80,,,2620502481,1914345031,2080364714,2597606056,2591318,ERX4268585,ERS4808142,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91412,0.91102,0.42824,0.4207,0.73091,0.73671,0.52094,0.52307,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9935,ERR4321726,ERX4268584,ERS4808141,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl uni 1,SAMEA7047507,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047507|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl uni 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl uni 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl uni 1 p,3dpl uni 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_uni_1_1.txt 3dpl_uni_1_2.txt,fastq fastq,9713477280.0,60709233.0,E MTAB 9321:3dpl uni 1 ,0:80 1:80,A:2666518127;C:1984727973;G:2436235221;T:2623269258;N:2726701,80,80,,,2666518127,1984727973,2436235221,2623269258,2726701,ERX4268584,ERS4808141,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91468,0.89921,0.41764,0.40764,0.73403,0.74608,0.52391,0.52748,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9936,ERR4321725,ERX4268583,ERS4808140,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl uni 3,SAMEA7047506,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047506|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl uni 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl uni 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl uni 3 p,20hpl uni 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_uni_3_1.txt 20hpl_uni_3_2.txt,fastq fastq,8675853280.0,54224083.0,E MTAB 9321:20hpl uni 3 ,0:80 1:80,A:2365631089;C:1890091876;G:2067595442;T:2325000100;N:27534773,80,80,,,2365631089,1890091876,2067595442,2325000100,27534773,ERX4268583,ERS4808140,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.90101,0.90675,0.42722,0.4249,0.74337,0.75294,0.51417,0.52085,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9937,ERR4321724,ERX4268582,ERS4808139,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl uni 2,SAMEA7047505,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047505|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl uni 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl uni 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl uni 2 p,20hpl uni 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_uni_2_1.txt 20hpl_uni_2_2.txt,fastq fastq,4735091040.0,29594319.0,E MTAB 9321:20hpl uni 2 ,0:80 1:80,A:1061979324;C:1276598224;G:1297035795;T:1098757554;N:720143,80,80,,,1061979324,1276598224,1297035795,1098757554,720143,ERX4268582,ERS4808139,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.9384,0.94067,0.25644,0.25853,0.7877,0.78843,0.69474,0.72221,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9938,ERR4321723,ERX4268581,ERS4808138,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl uni 1,SAMEA7047504,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047504|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl uni 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl uni 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl uni 1 p,20hpl uni 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_uni_1_1.txt 20hpl_uni_1_2.txt,fastq fastq,10122915040.0,63268219.0,E MTAB 9321:20hpl uni 1 ,0:80 1:80,A:2917523358;C:2094720604;G:2205586268;T:2872641443;N:32443367,80,80,,,2917523358,2094720604,2205586268,2872641443,32443367,ERX4268581,ERS4808138,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.90867,0.91132,0.46283,0.46241,0.74858,0.75357,0.53145,0.53522,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9939,ERR4321722,ERX4268580,ERS4808137,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in IWR 3,SAMEA7047503,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047503|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in IWR 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in IWR 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in IWR 3 p,3dpl in IWR 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_IWR_3_1.txt 3dpl_in_IWR_3_2.txt,fastq fastq,9476807840.0,59230049.0,E MTAB 9321:3dpl in IWR 3 ,0:80 1:80,A:2639849526;C:1994343199;G:2238787041;T:2601193111;N:2634963,80,80,,,2639849526,1994343199,2238787041,2601193111,2634963,ERX4268580,ERS4808137,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91666,0.90016,0.41957,0.41098,0.72679,0.73596,0.52875,0.53529,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9940,ERR4321721,ERX4268579,ERS4808136,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in IWR 2,SAMEA7047502,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047502|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in IWR 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in IWR 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in IWR 2 p,3dpl in IWR 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_IWR_2_1.txt 3dpl_in_IWR_2_2.txt,fastq fastq,7305898240.0,45661864.0,E MTAB 9321:3dpl in IWR 2 ,0:80 1:80,A:2035812198;C:1535985603;G:1714961805;T:2017139933;N:1998701,80,80,,,2035812198,1535985603,1714961805,2017139933,1998701,ERX4268579,ERS4808136,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91721,0.90787,0.40247,0.39455,0.72916,0.74097,0.52622,0.52732,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9941,ERR4321720,ERX4268578,ERS4808135,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in IWR 1,SAMEA7047501,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047501|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in IWR 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in IWR 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in IWR 1 p,3dpl in IWR 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_IWR_1_1.txt 3dpl_in_IWR_1_2.txt,fastq fastq,5893946400.0,36837165.0,E MTAB 9321:3dpl in IWR 1 ,0:80 1:80,A:1170533263;C:1737932152;G:1758304403;T:1226290662;N:885920,80,80,,,1170533263,1737932152,1758304403,1226290662,885920,ERX4268578,ERS4808135,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.95436,0.95726,0.17474,0.17743,0.77031,0.7726,0.72793,0.73265,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9942,ERR4321719,ERX4268577,ERS4808134,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in IWR 3,SAMEA7047500,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047500|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in IWR 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in IWR 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in IWR 3 p,20hpl in IWR 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_IWR_3_1.txt 20hpl_in_IWR_3_2.txt,fastq fastq,6198105920.0,38738162.0,E MTAB 9321:20hpl in IWR 3 ,0:80 1:80,A:1441128066;C:1626141975;G:1647720111;T:1482170340;N:945428,80,80,,,1441128066,1626141975,1647720111,1482170340,945428,ERX4268577,ERS4808134,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.94334,0.94604,0.2532,0.25458,0.75941,0.76086,0.66584,0.67193,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9943,ERR4321718,ERX4268576,ERS4808133,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in IWR 2,SAMEA7047499,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047499|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in IWR 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in IWR 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in IWR 2 p,20hpl in IWR 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_IWR_2_1.txt 20hpl_in_IWR_2_2.txt,fastq fastq,8244868480.0,51530428.0,E MTAB 9321:20hpl in IWR 2 ,0:80 1:80,A:2326026663;C:1706321438;G:1913900194;T:2272224793;N:26395392,80,80,,,2326026663,1706321438,1913900194,2272224793,26395392,ERX4268576,ERS4808133,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91275,0.90955,0.44535,0.44255,0.74456,0.75298,0.53931,0.53823,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9944,ERR4321717,ERX4268575,ERS4808132,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in IWR 1,SAMEA7047498,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047498|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in IWR 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in IWR 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in IWR 1 p,20hpl in IWR 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_IWR_1_1.txt 20hpl_in_IWR_1_2.txt,fastq fastq,8946910240.0,55918189.0,E MTAB 9321:20hpl in IWR 1 ,0:80 1:80,A:2523280880;C:1876368998;G:2029673577;T:2489111763;N:28475022,80,80,,,2523280880,1876368998,2029673577,2489111763,28475022,ERX4268575,ERS4808132,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91067,0.90286,0.45601,0.44987,0.7335,0.74119,0.51443,0.51662,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9945,ERR4321716,ERX4268574,ERS4808131,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in 3,SAMEA7047497,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047497|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in 3 p,3dpl in 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_3_1.txt 3dpl_in_3_2.txt,fastq fastq,9556572640.0,59728579.0,E MTAB 9321:3dpl in 3 ,0:80 1:80,A:2669191918;C:1902750470;G:2361798477;T:2620161228;N:2670547,80,80,,,2669191918,1902750470,2361798477,2620161228,2670547,ERX4268574,ERS4808131,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91175,0.89329,0.42236,0.413,0.72671,0.74156,0.52222,0.52828,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9946,ERR4321715,ERX4268573,ERS4808130,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in 2,SAMEA7047496,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:28Z|External Id:SAMEA7047496|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:28Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in 2 p,3dpl in 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_2_1.txt 3dpl_in_2_2.txt,fastq fastq,10848117120.0,67800732.0,E MTAB 9321:3dpl in 2 ,0:80 1:80,A:2935795866;C:2381506213;G:2652994352;T:2874780262;N:3040427,80,80,,,2935795866,2381506213,2652994352,2874780262,3040427,ERX4268573,ERS4808130,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91965,0.89867,0.42158,0.41268,0.73342,0.74249,0.56938,0.56978,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9947,ERR4321714,ERX4268572,ERS4808129,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in 1,SAMEA7047495,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:28Z|External Id:SAMEA7047495|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:28Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in 1 p,3dpl in 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_1_1.txt 3dpl_in_1_2.txt,fastq fastq,12371488960.0,77321806.0,E MTAB 9321:3dpl in 1 ,0:80 1:80,A:3438091571;C:2664088451;G:2872543859;T:3393310483;N:3454596,80,80,,,3438091571,2664088451,2872543859,3393310483,3454596,ERX4268572,ERS4808129,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.92096,0.90884,0.39682,0.3843,0.72614,0.73401,0.53892,0.53838,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9948,ERR4321713,ERX4268571,ERS4808128,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in 3,SAMEA7047494,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:28Z|External Id:SAMEA7047494|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:28Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in 3 p,20hpl in 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_3_1.txt 20hpl_in_3_2.txt,fastq fastq,10464739040.0,65404619.0,E MTAB 9321:20hpl in 3 ,0:80 1:80,A:2995469080;C:2169447356;G:2318878018;T:2947444090;N:33500496,80,80,,,2995469080,2169447356,2318878018,2947444090,33500496,ERX4268571,ERS4808128,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.9098,0.91109,0.46632,0.4653,0.74276,0.75077,0.51527,0.52148,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9949,ERR4321712,ERX4268570,ERS4808127,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in 2,SAMEA7047493,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:28Z|External Id:SAMEA7047493|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:28Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in 2 p,20hpl in 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_2_1.txt 20hpl_in_2_2.txt,fastq fastq,8520377280.0,53252358.0,E MTAB 9321:20hpl in 2 ,0:80 1:80,A:2416849489;C:1735946163;G:1978475279;T:2361749789;N:27356560,80,80,,,2416849489,1735946163,1978475279,2361749789,27356560,ERX4268570,ERS4808127,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91434,0.91,0.42899,0.42662,0.73917,0.74876,0.52201,0.51826,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9950,ERR4321711,ERX4268569,ERS4808126,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover  we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in 1,SAMEA7047492,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:28Z|External Id:SAMEA7047492|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:28Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in 1 p,20hpl in 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_1_1.txt 20hpl_in_1_2.txt,fastq fastq,7150452800.0,44690330.0,E MTAB 9321:20hpl in 1 ,0:80 1:80,A:1771803292;C:1779551247;G:1798541378;T:1799460160;N:1096723,80,80,,,1771803292,1779551247,1798541378,1799460160,1096723,ERX4268569,ERS4808126,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.92622,0.93038,0.35018,0.35291,0.7727,0.77396,0.64402,0.65297,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
10206,ERR6474244,ERX6101519,ERS7377049,ERP131171,PRJEB46937,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E-MTAB-10834,Other,RAP seq is a new method that provides in vitro derived RNA Interactomes for any given RBP. In RAP seq a recombinant RBP is produced as fusion with a HaloTag which is used to recover and purify the RBP of interest. The RBP Halo fusion is then incubated with fragmented total RNA derived from any given sample of interest. The bound RNA fragments are subsequently eluted and cloned using a small RNA library preparation protocol for sequencing the pool of bound molecules on an Illumina NGS platform.   In this study  RAP seq was used to identify RNA Interactomes of 26 novel RBPs aka non canonical RBPs newly discovered in proteome wide studies as RNA binders. RAP seq was also used to profile vertebrate HuR orthologs and described the biochemical evolutionary differences and similarities of the 6 orthologs profiled. Cancer associated IGF2BP1  IGF2BP2 and IGF2BP3 variants were also profiled and transcriptome wide changes in their RNA Interactomes with respect to the wild type IGF2BPs were reported. Also a transcriptome wide cooperative binding assay was perfomed to evaluate the cooperative roles of HuR and PTBP1 in binding to their native RNA targets.  In addition  a typical RAP seq substrate fragmented total RNA if reverse transcribed into cDNA and than in vitro transcribed again using a T7 RNA Polymerase can be depleted of any native endogenous RNA modifications and RAP seq assyas perfomed in parallel with the native substrate and the T7 RNAP produced one allowed us to discern the m6A dependency in transcriptome wide binding events for YTHDF1  hence with RAP seq we also report T7 RAP seq.,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,Protocols: HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library. Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,InputFISH,E MTAB 10834:InputFISH,,isolate:not applicable|disease:normal|ENA FIRST PUBLIC:2023 08 11T00:23:05Z|organism:Danio rerio|organism:Danio rerio|ENA LAST UPDATE:2023 08 11T00:23:05Z|cell line:1|scientific name:Danio rerio|common name:zebrafish|organism part:liver|cell type:hepatocyte|genotype:wild type genotype|ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,,,,,,,,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E MTAB 10834:InputFISH p,InputFISH p,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul  Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library.  Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,Experimental Factor: immunoprecipitate:input DNA|Experimental Factor: organism:Danio rerio,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 550,,ERP131171,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,InputFISH.R1.fastq.gz InputFISH.R2.fastq.gz,fastq fastq,2014534500.0,24271500.0,E MTAB 10834:InputFISH.R,0:40 1:43,A:435725523;C:539145149;G:601092466;T:438298953;N:272409,40,43,,,435725523,539145149,601092466,438298953,272409,ERX6101519,ERS7377049,ERA5607540,"Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive","Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive",2,0.394,0.34957,0.09053,0.09378,0.96485,0.97323,0.73467,0.62676,40,43,B,B,biological fallback assumption,illumina,nextseq,unknown,small_rna,unknown,bulk,unknown,unknown,,Sweden,2023-08-11,Undetermined,Undetermined,Multi-tissue,Multi-system
10207,ERR6474223,ERX6101498,ERS7377028,ERP131171,PRJEB46937,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E-MTAB-10834,Other,RAP seq is a new method that provides in vitro derived RNA Interactomes for any given RBP. In RAP seq a recombinant RBP is produced as fusion with a HaloTag which is used to recover and purify the RBP of interest. The RBP Halo fusion is then incubated with fragmented total RNA derived from any given sample of interest. The bound RNA fragments are subsequently eluted and cloned using a small RNA library preparation protocol for sequencing the pool of bound molecules on an Illumina NGS platform.   In this study  RAP seq was used to identify RNA Interactomes of 26 novel RBPs aka non canonical RBPs newly discovered in proteome wide studies as RNA binders. RAP seq was also used to profile vertebrate HuR orthologs and described the biochemical evolutionary differences and similarities of the 6 orthologs profiled. Cancer associated IGF2BP1  IGF2BP2 and IGF2BP3 variants were also profiled and transcriptome wide changes in their RNA Interactomes with respect to the wild type IGF2BPs were reported. Also a transcriptome wide cooperative binding assay was perfomed to evaluate the cooperative roles of HuR and PTBP1 in binding to their native RNA targets.  In addition  a typical RAP seq substrate fragmented total RNA if reverse transcribed into cDNA and than in vitro transcribed again using a T7 RNA Polymerase can be depleted of any native endogenous RNA modifications and RAP seq assyas perfomed in parallel with the native substrate and the T7 RNAP produced one allowed us to discern the m6A dependency in transcriptome wide binding events for YTHDF1  hence with RAP seq we also report T7 RAP seq.,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,Protocols: HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library. Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,hsHuRFISH rep2,E MTAB 10834:hsHuRFISH rep2,,isolate:not applicable|disease:normal|ENA FIRST PUBLIC:2023 08 11T00:23:05Z|organism:Danio rerio|organism:Danio rerio|ENA LAST UPDATE:2023 08 11T00:23:05Z|cell line:1|scientific name:Danio rerio|common name:zebrafish|organism part:liver|cell type:hepatocyte|genotype:wild type genotype|ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,,,,,,,,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E MTAB 10834:hsHuRFISH rep2 p,hsHuRFISH rep2 p,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul  Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library.  Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,Experimental Factor: immunoprecipitate:anti hsHuR|Experimental Factor: organism:Danio rerio,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 550,,ERP131171,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,hsHuRFISH_rep2.R2.fastq.gz hsHuRFISH_rep2.R1.fastq.gz,fastq fastq,2106598100.0,25380700.0,E MTAB 10834:hsHuRFISH rep2.R,0:40 1:43,A:503141361;C:548220448;G:569927989;T:485014455;N:293847,40,43,,,503141361,548220448,569927989,485014455,293847,ERX6101498,ERS7377028,ERA5607540,"Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive","Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive",2,0.76055,0.70626,0.18785,0.18742,0.92387,0.93533,0.72831,0.65481,40,43,B,B,biological fallback assumption,illumina,nextseq,unknown,small_rna,unknown,bulk,unknown,unknown,,Sweden,2023-08-11,Undetermined,Undetermined,Multi-tissue,Multi-system
10208,ERR6474222,ERX6101497,ERS7377027,ERP131171,PRJEB46937,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E-MTAB-10834,Other,RAP seq is a new method that provides in vitro derived RNA Interactomes for any given RBP. In RAP seq a recombinant RBP is produced as fusion with a HaloTag which is used to recover and purify the RBP of interest. The RBP Halo fusion is then incubated with fragmented total RNA derived from any given sample of interest. The bound RNA fragments are subsequently eluted and cloned using a small RNA library preparation protocol for sequencing the pool of bound molecules on an Illumina NGS platform.   In this study  RAP seq was used to identify RNA Interactomes of 26 novel RBPs aka non canonical RBPs newly discovered in proteome wide studies as RNA binders. RAP seq was also used to profile vertebrate HuR orthologs and described the biochemical evolutionary differences and similarities of the 6 orthologs profiled. Cancer associated IGF2BP1  IGF2BP2 and IGF2BP3 variants were also profiled and transcriptome wide changes in their RNA Interactomes with respect to the wild type IGF2BPs were reported. Also a transcriptome wide cooperative binding assay was perfomed to evaluate the cooperative roles of HuR and PTBP1 in binding to their native RNA targets.  In addition  a typical RAP seq substrate fragmented total RNA if reverse transcribed into cDNA and than in vitro transcribed again using a T7 RNA Polymerase can be depleted of any native endogenous RNA modifications and RAP seq assyas perfomed in parallel with the native substrate and the T7 RNAP produced one allowed us to discern the m6A dependency in transcriptome wide binding events for YTHDF1  hence with RAP seq we also report T7 RAP seq.,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,Protocols: HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library. Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,hsHuRFISH rep1,E MTAB 10834:hsHuRFISH rep1,,isolate:not applicable|disease:normal|ENA FIRST PUBLIC:2023 08 11T00:23:05Z|organism:Danio rerio|organism:Danio rerio|ENA LAST UPDATE:2023 08 11T00:23:05Z|cell line:1|scientific name:Danio rerio|common name:zebrafish|organism part:liver|cell type:hepatocyte|genotype:wild type genotype|ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,,,,,,,,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E MTAB 10834:hsHuRFISH rep1 p,hsHuRFISH rep1 p,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul  Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library.  Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,Experimental Factor: immunoprecipitate:anti hsHuR|Experimental Factor: organism:Danio rerio,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 550,,ERP131171,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,hsHuRFISH_rep1.R1.fastq.gz hsHuRFISH_rep1.R2.fastq.gz,fastq fastq,2520623431.0,30368957.0,E MTAB 10834:hsHuRFISH rep1.R,0:40 1:43,A:604086659;C:653262791;G:679919989;T:582992802;N:361190,40,43,,,604086659,653262791,679919989,582992802,361190,ERX6101497,ERS7377027,ERA5607540,"Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive","Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive",2,0.76096,0.70783,0.18844,0.18848,0.92101,0.93275,0.7333,0.62616,40,43,B,B,biological fallback assumption,illumina,nextseq,unknown,small_rna,unknown,bulk,unknown,unknown,,Sweden,2023-08-11,Undetermined,Undetermined,Multi-tissue,Multi-system
10209,ERR6474221,ERX6101496,ERS7377026,ERP131171,PRJEB46937,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E-MTAB-10834,Other,RAP seq is a new method that provides in vitro derived RNA Interactomes for any given RBP. In RAP seq a recombinant RBP is produced as fusion with a HaloTag which is used to recover and purify the RBP of interest. The RBP Halo fusion is then incubated with fragmented total RNA derived from any given sample of interest. The bound RNA fragments are subsequently eluted and cloned using a small RNA library preparation protocol for sequencing the pool of bound molecules on an Illumina NGS platform.   In this study  RAP seq was used to identify RNA Interactomes of 26 novel RBPs aka non canonical RBPs newly discovered in proteome wide studies as RNA binders. RAP seq was also used to profile vertebrate HuR orthologs and described the biochemical evolutionary differences and similarities of the 6 orthologs profiled. Cancer associated IGF2BP1  IGF2BP2 and IGF2BP3 variants were also profiled and transcriptome wide changes in their RNA Interactomes with respect to the wild type IGF2BPs were reported. Also a transcriptome wide cooperative binding assay was perfomed to evaluate the cooperative roles of HuR and PTBP1 in binding to their native RNA targets.  In addition  a typical RAP seq substrate fragmented total RNA if reverse transcribed into cDNA and than in vitro transcribed again using a T7 RNA Polymerase can be depleted of any native endogenous RNA modifications and RAP seq assyas perfomed in parallel with the native substrate and the T7 RNAP produced one allowed us to discern the m6A dependency in transcriptome wide binding events for YTHDF1  hence with RAP seq we also report T7 RAP seq.,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,Protocols: HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library. Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,HaloFISH,E MTAB 10834:HaloFISH,,isolate:not applicable|disease:normal|ENA FIRST PUBLIC:2023 08 11T00:23:05Z|organism:Danio rerio|organism:Danio rerio|ENA LAST UPDATE:2023 08 11T00:23:05Z|cell line:1|scientific name:Danio rerio|common name:zebrafish|organism part:liver|cell type:hepatocyte|genotype:wild type genotype|ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,,,,,,,,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E MTAB 10834:HaloFISH p,HaloFISH p,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul  Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library.  Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,Experimental Factor: immunoprecipitate:anti HaloTag|Experimental Factor: organism:Danio rerio,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 550,,ERP131171,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,HaloFISH.R2.fastq.gz HaloFISH.R1.fastq.gz,fastq fastq,2353027590.0,28349730.0,E MTAB 10834:HaloFISH.R,0:40 1:43,A:522964401;C:650152942;G:677020220;T:502555313;N:334714,40,43,,,522964401,650152942,677020220,502555313,334714,ERX6101496,ERS7377026,ERA5607540,"Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive","Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive",2,0.82307,0.78117,0.18309,0.18609,0.93888,0.94627,0.73861,0.63812,40,43,B,B,biological fallback assumption,illumina,nextseq,unknown,small_rna,unknown,bulk,unknown,unknown,,Sweden,2023-08-11,Undetermined,Undetermined,Multi-tissue,Multi-system
10210,ERR6474216,ERX6101492,ERS7377022,ERP131171,PRJEB46937,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E-MTAB-10834,Other,RAP seq is a new method that provides in vitro derived RNA Interactomes for any given RBP. In RAP seq a recombinant RBP is produced as fusion with a HaloTag which is used to recover and purify the RBP of interest. The RBP Halo fusion is then incubated with fragmented total RNA derived from any given sample of interest. The bound RNA fragments are subsequently eluted and cloned using a small RNA library preparation protocol for sequencing the pool of bound molecules on an Illumina NGS platform.   In this study  RAP seq was used to identify RNA Interactomes of 26 novel RBPs aka non canonical RBPs newly discovered in proteome wide studies as RNA binders. RAP seq was also used to profile vertebrate HuR orthologs and described the biochemical evolutionary differences and similarities of the 6 orthologs profiled. Cancer associated IGF2BP1  IGF2BP2 and IGF2BP3 variants were also profiled and transcriptome wide changes in their RNA Interactomes with respect to the wild type IGF2BPs were reported. Also a transcriptome wide cooperative binding assay was perfomed to evaluate the cooperative roles of HuR and PTBP1 in binding to their native RNA targets.  In addition  a typical RAP seq substrate fragmented total RNA if reverse transcribed into cDNA and than in vitro transcribed again using a T7 RNA Polymerase can be depleted of any native endogenous RNA modifications and RAP seq assyas perfomed in parallel with the native substrate and the T7 RNAP produced one allowed us to discern the m6A dependency in transcriptome wide binding events for YTHDF1  hence with RAP seq we also report T7 RAP seq.,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,Protocols: HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library. Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,drHuRFISH rep2,E MTAB 10834:drHuRFISH rep2,,isolate:not applicable|disease:normal|ENA FIRST PUBLIC:2023 08 11T00:23:05Z|organism:Danio rerio|organism:Danio rerio|ENA LAST UPDATE:2023 08 11T00:23:05Z|cell line:1|scientific name:Danio rerio|common name:zebrafish|organism part:liver|cell type:hepatocyte|genotype:wild type genotype|ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,,,,,,,,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E MTAB 10834:drHuRFISH rep2 p,drHuRFISH rep2 p,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul  Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library.  Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,Experimental Factor: immunoprecipitate:anti drHuR|Experimental Factor: organism:Danio rerio,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 550,,ERP131171,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,drHuRFISH_rep2.R1.fastq.gz drHuRFISH_rep2.R2.fastq.gz,fastq fastq,2408024386.0,29012342.0,E MTAB 10834:drHuRFISH rep2.R,0:40 1:43,A:566287649;C:627743498;G:663052609;T:550604982;N:335648,40,43,,,566287649,627743498,663052609,550604982,335648,ERX6101492,ERS7377022,ERA5607540,"Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive","Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive",2,0.68524,0.62721,0.16916,0.1656,0.93501,0.94541,0.7028,0.64738,40,43,B,B,biological fallback assumption,illumina,nextseq,unknown,small_rna,unknown,bulk,unknown,unknown,,Sweden,2023-08-11,Undetermined,Undetermined,Multi-tissue,Multi-system
10211,ERR6474215,ERX6101491,ERS7377021,ERP131171,PRJEB46937,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E-MTAB-10834,Other,RAP seq is a new method that provides in vitro derived RNA Interactomes for any given RBP. In RAP seq a recombinant RBP is produced as fusion with a HaloTag which is used to recover and purify the RBP of interest. The RBP Halo fusion is then incubated with fragmented total RNA derived from any given sample of interest. The bound RNA fragments are subsequently eluted and cloned using a small RNA library preparation protocol for sequencing the pool of bound molecules on an Illumina NGS platform.   In this study  RAP seq was used to identify RNA Interactomes of 26 novel RBPs aka non canonical RBPs newly discovered in proteome wide studies as RNA binders. RAP seq was also used to profile vertebrate HuR orthologs and described the biochemical evolutionary differences and similarities of the 6 orthologs profiled. Cancer associated IGF2BP1  IGF2BP2 and IGF2BP3 variants were also profiled and transcriptome wide changes in their RNA Interactomes with respect to the wild type IGF2BPs were reported. Also a transcriptome wide cooperative binding assay was perfomed to evaluate the cooperative roles of HuR and PTBP1 in binding to their native RNA targets.  In addition  a typical RAP seq substrate fragmented total RNA if reverse transcribed into cDNA and than in vitro transcribed again using a T7 RNA Polymerase can be depleted of any native endogenous RNA modifications and RAP seq assyas perfomed in parallel with the native substrate and the T7 RNAP produced one allowed us to discern the m6A dependency in transcriptome wide binding events for YTHDF1  hence with RAP seq we also report T7 RAP seq.,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,Protocols: HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library. Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,drHuRFISH rep1,E MTAB 10834:drHuRFISH rep1,,isolate:not applicable|disease:normal|ENA FIRST PUBLIC:2023 08 11T00:23:05Z|organism:Danio rerio|organism:Danio rerio|ENA LAST UPDATE:2023 08 11T00:23:05Z|cell line:1|scientific name:Danio rerio|common name:zebrafish|organism part:liver|cell type:hepatocyte|genotype:wild type genotype|ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,,,,,,,,,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,E MTAB 10834:drHuRFISH rep1 p,drHuRFISH rep1 p,RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,HepG2 were obtained from American Type Culture Collection ATCC  Rockville  MD. The cell line was mycoplasma free when periodically tested with Mycoplasmacheck Eurofins Genomics. HepG2 were cultured in T75 flasks at 37 °C and 5 % CO2 atmosphere using Dulbecco's Modified Eagle Medium DMEM supplemented with 1/100 Penicillin/Streptomycin P/S  Sigma and 10 % fetal bovine serum Hyclone  GE healthcare. HepG2 cells were maintained by splitting 1/6 three times a week  done by aspirating the medium  gently washing the cells with phosphate buffered saline PBS  without xxx+  Sigma and detaching them with 2 mL of a trypsin EDTA solution 0.05%  Sigma for 3 5 min. Trypsin EDTA was inactivated with a minimum of 10 fold surplus of culture medium before a cell fraction was passaged Substrate preparation: DNAse treated and column cleaned total RNA was fragmeted at 95 degrees celsius in Tris HCl 80 mM and MgCl2 8 mM final concentrations for 4 minutes and 30 seconds. post fragmentation  the RNA was cleaned using 1 volume of RNA CleanXP Ampure beads and 1 volume of isopropanol; post 2 80% Ethanol washes the RNA was eluted in half the volume of AMPure beads used. three prime dephosphorylation was then performed with T4PNK in TAM buffer Tris Acetate 50 mM  Magnesium Chloride 10 mM  pH8.5 at room temperature for 4 hours followed by AMPure clean up and subsequently five prime phosphorylated with T4PNK in T4PNK buffer with addition of ATP at 37 degreees celsius for 1 hour according to NEB manufacturer instructions  then AMPure cleaned and aliquoted and stored at  80 degrees celsius until further used. Binding Assay: RBP Halo fusions are in vitro transcribed and then translated with MegaScript T7 in vitro transcription kit ThermoFisher and Wheat Germ extract according to manufacturers instructions 50 ul in vitro translation reaction. The in vitro translated proteins are recovered using 25 ul  Magne Halo Ligand beads Promega washed and resuspended in PBSN 1X PBS  0 005% NP 40 with added RNAse A and Turbo DNAse Thermo. post 1 hour of incubation  the beads are washed 4 times  twice with high salt PBSN + 1M MgCl2 and twice with normal salt PBSN. Beads are ruspended in 50 ul of PBSN and mixed with one aliquote of substrate RNA 150 ng in total brought to room temperature and diluted to a final volume of 50 ul of PBSN. The binding reaction occurs at room temperature for one hour rotating end over end subsequently 3 washes in PBSN are performed to remove unbound molecules and elution is perfomed in 15 ul of RNAse free water at 70 degrees celsius for 8 minutes resuspending the beads by pipeting up and down whilst the tube is in the thermocycler for a total of 3 times during the 8 minutes at 70 degrees. 11 ul of the eluted RNA are used to prepare an illumina compatible next generation sequencing library.  Upon cell harvest  700 µL Qiazol QIAGEN was directly added onto the cells on ice and mixed. The cell extract was either stored at  80 °C or RNA extraction was continued immediately by adding 140 µL chloroform. This Qiazol/chloroform mixture was shaken for 30 sec and incubated at RT for 3 min  before centrifugation at 9.000 g for 5 min at 4 °C. The upper aqueous phase was transferred to a new tube and an equal volume of isopropanol was added. The tube was inverted 5 times followed by 10 min incubation at RT. The mixture was centrifuged at 9.000 g and 4 °C for 10 min and the supernatant discarded. The pellet was washed in 700 µL cold 70% ethanol and centrifuged at 15.000 g for 5 min at 4 °C. The supernatant was discarded entirely  and the pellet air dried for 5 minutes  before resuspension in nuclease free water. RNA concentration was determined by nanodrop Nanodrop 2000c. RNA was DNase treated using the Turbo DNase Kit Thermo Fisher for 30 min at 37 °C and the reaction was column purified using RNA Clean&Concentrator kit Zymo Research. The library preparation was carried out using the NEXTFLEX small RNA library preparation kit v3  PerkinElmer catalog # NOVA 5132 06 according to manufacturer instructions. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol.,Experimental Factor: immunoprecipitate:anti drHuR|Experimental Factor: organism:Danio rerio,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 550,,ERP131171,NextSeq 550 paired end sequencing; RNA Affinity Purification followed by sequencing RAP seq allows identification of transcriptome wide RBP binding sites using recombinant RBPs and total purified cellular RNA,ENA FIRST PUBLIC:2023 08 11|ENA LAST UPDATE:2023 08 11,drHuRFISH_rep1.R1.fastq.gz drHuRFISH_rep1.R2.fastq.gz,fastq fastq,3025188980.0,36448060.0,E MTAB 10834:drHuRFISH rep1.R,0:40 1:43,A:720153781;C:784188971;G:824077525;T:696330222;N:438481,40,43,,,720153781,784188971,824077525,696330222,438481,ERX6101491,ERS7377021,ERA5607540,"Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive","Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute, Science for Life Laboratory, Sweden|European Nucleotide Archive",2,0.7001,0.64217,0.17626,0.1728,0.93154,0.94249,0.72494,0.65821,40,43,B,B,biological fallback assumption,illumina,nextseq,unknown,small_rna,unknown,bulk,unknown,unknown,,Sweden,2023-08-11,Undetermined,Undetermined,Multi-tissue,Multi-system
10214,ERR6617900,ERX6244443,ERS7291130,ERP131213,PRJEB46978,Nano3P seq: transcriptome wide analysis of gene expression and tail dynamics using end capture nanopore sequencing,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-12-08-2021-14:48:52:906-1159,Other,Nano3P seq is a simple and robust method to accurately estimate transcript levels  tail lengths and tail nucleotide composition information in full length individual reads  with minimal library preparation biases  both in the coding and non coding transcriptome.,ENA FIRST PUBLIC:2023 12 28|ENA LAST UPDATE:2023 12 28,,PolyA selected dRNA sequenced zebrafish 4hpf RNA,Zebrafish 4hpf dRNA,SAMEA9568396,CENTER FOR GENOMIC REGULATION (CRG),ENA FIRST PUBLIC:2023 12 28T01:07:30Z|ENA LAST UPDATE:2023 12 28T01:07:30Z|External Id:SAMEA9568396|INSDC center name:CENTER FOR GENOMIC REGULATION CRG|INSDC first public:2023 12 28T01:07:30Z|INSDC last update:2023 12 28T01:07:30Z|INSDC status:public|Submitter Id:Zebrafish 4hpf dRNA1|common name:zebrafish|sample name:Zebrafish 4hpf dRNA1|scientific name:Danio rerio,,,,,,,,,MinION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 03 09 2021 14:33:13:450 1,dRNA Zebrafish,Direct RNA Sequencing,Direct RNA Sequencing,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,OXFORD_NANOPORE,MinION,,ERP131213,MinION sequencing,ENA FIRST PUBLIC:2023 12 28|ENA LAST UPDATE:2023 12 28,Zebrafish_4hpf_dRNA.fast5.tar.gz,nanopore,772304625.0,897768.0,ena RUN CENTER FOR GENOMIC REGULATION CRG 03 09 2021 14:33:13:450 1,0:860.25,A:224977035;C:165273397;G:156659356;T:225394837;N:0,860,,,,224977035,165273397,156659356,225394837,0,ERX6244443,ERS7291130,ERA5995143,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),1,0.5,,0.0,,0.99997,,1.0,,962,,T,,long read,ont,ont,full_length,poly_a,unknown,bulk,unknown,unknown,,Spain,2023-12-28,Blastula,Embryo,Undetermined,Embryo Imprecise
10324,ERR7384926,ERX6953430,ERS8736631,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl 5,SAMEA11086968,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086968|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:Ctrl 5|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:telencephalon|sample name:E MTAB 11163:Ctrl 5|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:Ctrl 5 p,Ctrl 5 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,Ctrl-5_1.fastq.gz Ctrl-5_2.fastq.gz,fastq fastq,7836077760.0,48975486.0,E MTAB 11163:Ctrl 5 ,0:80 1:80,A:2197807782;C:1719033269;G:1762364798;T:2156195280;N:676631,80,80,,,2197807782,1719033269,1762364798,2156195280,676631,ERX6953430,ERS8736631,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.93458,0.93849,0.19317,0.18598,0.72855,0.72784,0.48113,0.4814,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10325,ERR7384925,ERX6953429,ERS8736630,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl 4,SAMEA11086967,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086967|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:Ctrl 4|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:telencephalon|sample name:E MTAB 11163:Ctrl 4|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:Ctrl 4 p,Ctrl 4 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,Ctrl-4_1.fastq.gz Ctrl-4_2.fastq.gz,fastq fastq,11841052800.0,74006580.0,E MTAB 11163:Ctrl 4 ,0:80 1:80,A:3269511229;C:2469072822;G:2920921930;T:3177156542;N:4390277,80,80,,,3269511229,2469072822,2920921930,3177156542,4390277,ERX6953429,ERS8736630,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.86759,0.86792,0.43044,0.42689,0.75469,0.76717,0.55291,0.55788,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10326,ERR7384924,ERX6953428,ERS8736629,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl 3,SAMEA11086966,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086966|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:Ctrl 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:telencephalon|sample name:E MTAB 11163:Ctrl 3|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:Ctrl 3 p,Ctrl 3 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,Ctrl-3_1.fastq.gz Ctrl-3_2.fastq.gz,fastq fastq,14286470240.0,89290439.0,E MTAB 11163:Ctrl 3 ,0:80 1:80,A:3956940377;C:3071545407;G:3372465520;T:3880155204;N:5363732,80,80,,,3956940377,3071545407,3372465520,3880155204,5363732,ERX6953428,ERS8736629,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.87493,0.87449,0.43889,0.44065,0.7541,0.76402,0.5369,0.53575,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10327,ERR7384923,ERX6953427,ERS8736628,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl 2,SAMEA11086965,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086965|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:Ctrl 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:telencephalon|sample name:E MTAB 11163:Ctrl 2|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:Ctrl 2 p,Ctrl 2 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,Ctrl-2_1.fastq.gz Ctrl-2_2.fastq.gz,fastq fastq,13169862240.0,82311639.0,E MTAB 11163:Ctrl 2 ,0:80 1:80,A:3683560119;C:2823399426;G:3030116974;T:3627855699;N:4930022,80,80,,,3683560119,2823399426,3030116974,3627855699,4930022,ERX6953427,ERS8736628,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.86757,0.87095,0.44548,0.45008,0.75536,0.76343,0.53366,0.54558,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10328,ERR7384922,ERX6953426,ERS8736627,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl 1,SAMEA11086964,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086964|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:Ctrl 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:telencephalon|sample name:E MTAB 11163:Ctrl 1|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:Ctrl 1 p,Ctrl 1 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,Ctrl-1_1.fastq.gz Ctrl-1_2.fastq.gz,fastq fastq,11349398560.0,70933741.0,E MTAB 11163:Ctrl 1 ,0:80 1:80,A:3132234873;C:2275439795;G:2903048149;T:3034427880;N:4247863,80,80,,,3132234873,2275439795,2903048149,3034427880,4247863,ERX6953426,ERS8736627,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.86563,0.85769,0.45807,0.45717,0.75542,0.76999,0.53285,0.52788,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10329,ERR7384921,ERX6953425,ERS8736626,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl 4,SAMEA11086962,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086962|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:3dpl 4|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|injury:stab lesion|organism part:telencephalon|sample name:E MTAB 11163:3dpl 4|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:3dpl 4 p,3dpl 4 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,3dpl-4_1.fastq.gz 3dpl-4_2.fastq.gz,fastq fastq,12269133600.0,76682085.0,E MTAB 11163:3dpl 4 ,0:80 1:80,A:3308670822;C:2612099729;G:3097254260;T:3246547111;N:4561678,80,80,,,3308670822,2612099729,3097254260,3246547111,4561678,ERX6953425,ERS8736626,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.88949,0.88423,0.41382,0.41463,0.73856,0.75209,0.54087,0.53814,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10330,ERR7384920,ERX6953424,ERS8736625,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl 3,SAMEA11086961,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086961|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:3dpl 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|injury:stab lesion|organism part:telencephalon|sample name:E MTAB 11163:3dpl 3|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:3dpl 3 p,3dpl 3 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,3dpl-3_1.fastq.gz 3dpl-3_2.fastq.gz,fastq fastq,12561832800.0,78511455.0,E MTAB 11163:3dpl 3 ,0:80 1:80,A:3518655485;C:2691495460;G:2879365772;T:3467606057;N:4710026,80,80,,,3518655485,2691495460,2879365772,3467606057,4710026,ERX6953424,ERS8736625,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.86128,0.86324,0.43991,0.44309,0.73998,0.74761,0.5283,0.52762,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10331,ERR7384919,ERX6953423,ERS8736624,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl 2,SAMEA11086960,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086960|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:3dpl 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|injury:stab lesion|organism part:telencephalon|sample name:E MTAB 11163:3dpl 2|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:3dpl 2 p,3dpl 2 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,3dpl-2_1.fastq.gz 3dpl-2_2.fastq.gz,fastq fastq,12727937600.0,79549610.0,E MTAB 11163:3dpl 2 ,0:80 1:80,A:3511550580;C:2718113416;G:3063150762;T:3430381211;N:4741631,80,80,,,3511550580,2718113416,3063150762,3430381211,4741631,ERX6953423,ERS8736624,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.88126,0.88294,0.42008,0.41964,0.72687,0.73811,0.52924,0.52812,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10332,ERR7384918,ERX6953422,ERS8736623,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl 1,SAMEA11086959,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086959|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:3dpl 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|injury:stab lesion|organism part:telencephalon|sample name:E MTAB 11163:3dpl 1|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:3dpl 1 p,3dpl 1 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,3dpl-1_1.fastq.gz 3dpl-1_2.fastq.gz,fastq fastq,10910301440.0,68189384.0,E MTAB 11163:3dpl 1 ,0:80 1:80,A:2970902938;C:2403780256;G:2602413517;T:2929186974;N:4017755,80,80,,,2970902938,2403780256,2602413517,2929186974,4017755,ERX6953422,ERS8736623,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.84003,0.84172,0.34597,0.34824,0.71589,0.72466,0.52558,0.52286,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10333,ERR7384917,ERX6953421,ERS8736622,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,1dpl 5,SAMEA11086958,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086958|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:1dpl 5|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|injury:stab lesion|organism part:telencephalon|sample name:E MTAB 11163:1dpl 5|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:1dpl 5 p,1dpl 5 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: time:1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,1dpl-5_1.fastq.gz 1dpl-5_2.fastq.gz,fastq fastq,10425544320.0,65159652.0,E MTAB 11163:1dpl 5 ,0:80 1:80,A:3050099743;C:2153241581;G:2229178430;T:2992123587;N:900979,80,80,,,3050099743,2153241581,2229178430,2992123587,900979,ERX6953421,ERS8736622,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.92853,0.93189,0.23718,0.23074,0.72366,0.72052,0.49924,0.511,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System
10334,ERR7384916,ERX6953420,ERS8736621,ERP133301,PRJEB48872,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,E-MTAB-11163,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study  we reveal the whole transcriptome profiles of the lesioned hemispheres of the zebrafish telencephalon at early wound healing 1 day post lesion  early proliferative 3 day post lesion and differentiation 14 day post lesion stages of regeneration.,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,1dpl 4,SAMEA11086957,Izmir Biomedicine and Genome Center,ENA first public:2022 01 31|ENA last update:2022 01 31|External Id:SAMEA11086957|INSDC center alias:Izmir Biomedicine and Genome Center|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2022 01 31T00:14:07Z|INSDC last update:2022 01 31T00:14:07Z|INSDC status:public|Submitter Id:E MTAB 11163:1dpl 4|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|injury:stab lesion|organism part:telencephalon|sample name:E MTAB 11163:1dpl 4|sex:male|strain:AB,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the 3 different stages of the regenerating zebrafish telencephalon,E MTAB 11163:1dpl 4 p,1dpl 4 p,Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later  and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: time:1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP133301,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the three different stages of the regenerating zebrafish telencephalon,ENA FIRST PUBLIC:2022 01 31|ENA LAST UPDATE:2022 01 31,1dpl-4_1.fastq.gz 1dpl-4_2.fastq.gz,fastq fastq,9244695360.0,57779346.0,E MTAB 11163:1dpl 4 ,0:80 1:80,A:2617398368;C:2006695457;G:2057821797;T:2561969157;N:810581,80,80,,,2617398368,2006695457,2057821797,2561969157,810581,ERX6953420,ERS8736621,ERA7396457,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.93687,0.94089,0.20045,0.19324,0.71674,0.71506,0.49841,0.50035,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2022-01-31,Adult,Adult,Brain,Nervous System