rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 33379,SRR30184285,SRX25650011,SRS22293080,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,cMO 2,wt cmo2 M,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:cMO 2|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L10,L10,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L10_S128_L005_R2_001.fastq.gz L10_S128_L005_R1_001.fastq.gz,fastq fastq,20328275944.0,67312172.0,L10 S128 L005 R1 001.fastq.gz,0:151 1:151,A:5643585269;C:4447562096;G:4432908344;T:5802780590;N:1439645,151,151,,,5643585269,4447562096,4432908344,5802780590,1439645,SRX25650011,SRS22293080,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.89472,0.89558,0.14231,0.14209,0.76359,0.76327,0.57442,0.57155,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33380,SRR30184286,SRX25650010,SRS22293078,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,cMO 1,wt cmo1 M,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:cMO 1|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L09,L09,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L09_S106_L004_R2_001.fastq.gz L09_S106_L004_R1_001.fastq.gz,fastq fastq,17931167252.0,59374726.0,L09 S106 L004 R1 001.fastq.gz,0:151 1:151,A:4724456408;C:4170780589;G:4192052775;T:4841803749;N:2073731,151,151,,,4724456408,4170780589,4192052775,4841803749,2073731,SRX25650010,SRS22293078,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.92527,0.92522,0.05941,0.05976,0.76532,0.76583,0.55358,0.55497,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33381,SRR30184287,SRX25650009,SRS22293079,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,3dpi 4,wt R2 F,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:3dpi 4|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L08,L08,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L08_S127_L005_R2_001.fastq.gz L08_S127_L005_R1_001.fastq.gz,fastq fastq,22665495016.0,75051308.0,L08 S127 L005 R1 001.fastq.gz,0:151 1:151,A:6228385739;C:4990124759;G:4979460238;T:6465924079;N:1600201,151,151,,,6228385739,4990124759,4979460238,6465924079,1600201,SRX25650009,SRS22293079,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.87648,0.87921,0.12966,0.13019,0.75627,0.75666,0.5783,0.5754,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33382,SRR30184288,SRX25650008,SRS22293077,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,3dpi 3,wt R1 F,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:3dpi 3|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L07,L07,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L07_S126_L005_R2_001.fastq.gz L07_S126_L005_R1_001.fastq.gz,fastq fastq,22406825976.0,74194788.0,L07 S126 L005 R1 001.fastq.gz,0:151 1:151,A:6090398182;C:4986126981;G:5056253833;T:6272474188;N:1572792,151,151,,,6090398182,4986126981,5056253833,6272474188,1572792,SRX25650008,SRS22293077,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.9153,0.91559,0.10005,0.0998,0.74679,0.7469,0.53845,0.5395,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33383,SRR30184289,SRX25650007,SRS22293075,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,3dpi 2,wt R2 M,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:3dpi 2|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L06,L06,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L06_S125_L005_R2_001.fastq.gz L06_S125_L005_R1_001.fastq.gz,fastq fastq,21918766796.0,72578698.0,L06 S125 L005 R1 001.fastq.gz,0:151 1:151,A:6030044320;C:4788979368;G:4790388904;T:6307807807;N:1546397,151,151,,,6030044320,4788979368,4790388904,6307807807,1546397,SRX25650007,SRS22293075,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.87272,0.87475,0.15184,0.15299,0.76777,0.76702,0.58094,0.57863,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33384,SRR30184290,SRX25650006,SRS22293076,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,3dpi 1,wt R1 M,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:3dpi 1|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L05,L05,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L05_S124_L005_R1_001.fastq.gz L05_S124_L005_R2_001.fastq.gz,fastq fastq,20254563180.0,67068090.0,L05 S124 L005 R1 001.fastq.gz,0:151 1:151,A:5433584775;C:4559357895;G:4610242789;T:5649933621;N:1444100,151,151,,,5433584775,4559357895,4610242789,5649933621,1444100,SRX25650006,SRS22293076,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.90628,0.90764,0.08395,0.08422,0.7712,0.77086,0.5176,0.51705,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33385,SRR30184291,SRX25650005,SRS22293074,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,uninjured 4,wt L2 F,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:uninjured 4|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L04,L04,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L04_S142_L006_R2_001.fastq.gz L04_S142_L006_R1_001.fastq.gz,fastq fastq,29812977034.0,98718467.0,L04 S142 L006 R1 001.fastq.gz,0:151 1:151,A:8014887204;C:6674942655;G:6635556218;T:8483149485;N:4441472,151,151,,,8014887204,6674942655,6635556218,8483149485,4441472,SRX25650005,SRS22293074,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.84638,0.84891,0.16019,0.1609,0.77737,0.77733,0.62585,0.63219,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33386,SRR30184292,SRX25650004,SRS22293073,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,heatshock srebf2MO 4,hs smo2 F,,strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock srebf2MO 4|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L28,L28,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L28_S116_L004_R2_001.fastq.gz L28_S116_L004_R1_001.fastq.gz,fastq fastq,17026943012.0,56380606.0,L28 S116 L004 R1 001.fastq.gz,0:151 1:151,A:4669306614;C:3826935006;G:3777947200;T:4750777220;N:1976972,151,151,,,4669306614,3826935006,3777947200,4750777220,1976972,SRX25650004,SRS22293073,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.93284,0.93235,0.07802,0.07812,0.75568,0.75597,0.55431,0.54921,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33387,SRR30184293,SRX25650003,SRS22293072,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,heatshock srebf2MO 3,hs smo1 F,,strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock srebf2MO 3|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L27,L27,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L27_S115_L004_R2_001.fastq.gz L27_S115_L004_R1_001.fastq.gz,fastq fastq,22610315690.0,74868595.0,L27 S115 L004 R1 001.fastq.gz,0:151 1:151,A:6205491974;C:5075129196;G:4965212019;T:6361870398;N:2612103,151,151,,,6205491974,5075129196,4965212019,6361870398,2612103,SRX25650003,SRS22293072,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.78769,0.78807,0.09282,0.09299,0.76619,0.7651,0.54884,0.5509,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33388,SRR30184294,SRX25650002,SRS22293071,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,heatshock srebf2MO 2,hs smo2 M,,strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock srebf2MO 2|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L26,L26,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L26_S144_L006_R2_001.fastq.gz L26_S144_L006_R1_001.fastq.gz,fastq fastq,17129465368.0,56720084.0,L26 S144 L006 R1 001.fastq.gz,0:151 1:151,A:4750698191;C:3734802838;G:3895289691;T:4746170435;N:2504213,151,151,,,4750698191,3734802838,3895289691,4746170435,2504213,SRX25650002,SRS22293071,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.93654,0.92792,0.07609,0.07622,0.75061,0.75357,0.52583,0.53272,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33389,SRR30184295,SRX25650001,SRS22293070,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,heatshock srebf2MO 1,hs smo1 M,,strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock srebf2MO 1|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L25,L25,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L25_S114_L004_R2_001.fastq.gz L25_S114_L004_R1_001.fastq.gz,fastq fastq,18411870316.0,60966458.0,L25 S114 L004 R1 001.fastq.gz,0:151 1:151,A:5037571347;C:4144572498;G:4103153869;T:5124440844;N:2131758,151,151,,,5037571347,4144572498,4103153869,5124440844,2131758,SRX25650001,SRS22293070,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.92822,0.92792,0.0886,0.08918,0.75974,0.75978,0.55638,0.55618,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33390,SRR30184296,SRX25650000,SRS22293066,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,uninjured 3,wt L1 F,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:uninjured 3|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L03,L03,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L03_S105_L004_R1_001.fastq.gz L03_S105_L004_R2_001.fastq.gz,fastq fastq,19718182792.0,65291996.0,L03 S105 L004 R1 001.fastq.gz,0:151 1:151,A:5247234351;C:4504410887;G:4526181297;T:5438074144;N:2282113,151,151,,,5247234351,4504410887,4526181297,5438074144,2282113,SRX25650000,SRS22293066,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.90475,0.90699,0.11305,0.11331,0.75014,0.74929,0.52801,0.54316,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33391,SRR30184297,SRX25649999,SRS22293069,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,heatshock cMO 4,hs cmo2 F,,strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock cMO 4|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L24,L24,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L24_S134_L005_R2_001.fastq.gz L24_S134_L005_R1_001.fastq.gz,fastq fastq,19377661484.0,64164442.0,L24 S134 L005 R1 001.fastq.gz,0:151 1:151,A:5371834087;C:4284931090;G:4227428342;T:5492109103;N:1358862,151,151,,,5371834087,4284931090,4227428342,5492109103,1358862,SRX25649999,SRS22293069,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.9104,0.90922,0.11083,0.11062,0.75325,0.7543,0.54602,0.54303,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33392,SRR30184298,SRX25649998,SRS22293067,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,heatshock cMO 3,hs cmo1 F,,strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock cMO 3|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L23,L23,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L23_S143_L006_R2_001.fastq.gz L23_S143_L006_R1_001.fastq.gz,fastq fastq,28079439956.0,92978278.0,L23 S143 L006 R1 001.fastq.gz,0:151 1:151,A:7811720040;C:6084170964;G:6064272363;T:8115079585;N:4197004,151,151,,,7811720040,6084170964,6064272363,8115079585,4197004,SRX25649998,SRS22293067,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.86593,0.86525,0.14584,0.14587,0.77498,0.77433,0.59534,0.59433,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33393,SRR30184299,SRX25649997,SRS22293068,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,heatshock cMO 2,hs cmo2 M,,strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock cMO 2|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L22,L22,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L22_S133_L005_R2_001.fastq.gz L22_S133_L005_R1_001.fastq.gz,fastq fastq,20494382588.0,67862194.0,L22 S133 L005 R1 001.fastq.gz,0:151 1:151,A:5610165229;C:4583590395;G:4590759271;T:5708435247;N:1432446,151,151,,,5610165229,4583590395,4590759271,5708435247,1432446,SRX25649997,SRS22293068,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.92306,0.92279,0.0879,0.08765,0.7671,0.76579,0.56402,0.56401,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33394,SRR30184300,SRX25649996,SRS22293064,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,heatshock cMO 1,hs cmo1 M,,strain:Tghsp70l:nSrebf2 2AmCherry cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:heatshock cMO 1|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L21,L21,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L21_S113_L004_R1_001.fastq.gz L21_S113_L004_R2_001.fastq.gz,fastq fastq,22282038670.0,73781585.0,L21 S113 L004 R1 001.fastq.gz,0:151 1:151,A:5984432885;C:5103219794;G:5123288852;T:6068507910;N:2589229,151,151,,,5984432885,5103219794,5123288852,6068507910,2589229,SRX25649996,SRS22293064,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.93523,0.93442,0.06557,0.06558,0.76463,0.76532,0.54365,0.53711,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33395,SRR30184301,SRX25649995,SRS22293061,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,srebf2MO 4,wt smo2 F,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:srebf2MO 4|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L16,L16,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L16_S108_L004_R1_001.fastq.gz L16_S108_L004_R2_001.fastq.gz,fastq fastq,25364244630.0,83987565.0,L16 S108 L004 R1 001.fastq.gz,0:151 1:151,A:6783811492;C:5825885841;G:5791188806;T:6960421955;N:2936536,151,151,,,6783811492,5825885841,5791188806,6960421955,2936536,SRX25649995,SRS22293061,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.93174,0.93131,0.06871,0.06898,0.75461,0.75386,0.54103,0.54073,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33396,SRR30184302,SRX25649994,SRS22293065,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,srebf2MO 3,wt smo1 F,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:srebf2MO 3|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L15,L15,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L15_S132_L005_R2_001.fastq.gz L15_S132_L005_R1_001.fastq.gz,fastq fastq,24643120876.0,81599738.0,L15 S132 L005 R1 001.fastq.gz,0:151 1:151,A:6791560596;C:5441282614;G:5412453327;T:6996084050;N:1740289,151,151,,,6791560596,5441282614,5412453327,6996084050,1740289,SRX25649994,SRS22293065,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.90806,0.90874,0.11049,0.11033,0.75885,0.75741,0.54345,0.55215,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33397,SRR30184303,SRX25649993,SRS22293062,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,srebf2MO 2,wt smo2 M,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:srebf2MO 2|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L14,L14,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L14_S131_L005_R2_001.fastq.gz L14_S131_L005_R1_001.fastq.gz,fastq fastq,20984328060.0,69484530.0,L14 S131 L005 R1 001.fastq.gz,0:151 1:151,A:5728503785;C:4710613293;G:4687638412;T:5856111147;N:1461423,151,151,,,5728503785,4710613293,4687638412,5856111147,1461423,SRX25649993,SRS22293062,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.92516,0.92471,0.08416,0.08435,0.76568,0.76585,0.56286,0.56146,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33398,SRR30184304,SRX25649992,SRS22293059,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,srebf2MO 1,wt smo1 M,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:srebf2MO 1|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L13,L13,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L13_S130_L005_R1_001.fastq.gz L13_S130_L005_R2_001.fastq.gz,fastq fastq,17976563590.0,59525045.0,L13 S130 L005 R1 001.fastq.gz,0:151 1:151,A:4931490430;C:3978811459;G:3956968246;T:5108014615;N:1278840,151,151,,,4931490430,3978811459,3956968246,5108014615,1278840,SRX25649992,SRS22293059,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.8948,0.89491,0.13113,0.13061,0.76254,0.76246,0.57407,0.57069,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33399,SRR30184305,SRX25649991,SRS22293060,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,cMO 4,wt cmo2 F,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:cMO 4|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L12,L12,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L12_S129_L005_R2_001.fastq.gz L12_S129_L005_R1_001.fastq.gz,fastq fastq,19905894214.0,65913557.0,L12 S129 L005 R1 001.fastq.gz,0:151 1:151,A:5382577098;C:4496451576;G:4493042842;T:5532424959;N:1397739,151,151,,,5382577098,4496451576,4493042842,5532424959,1397739,SRX25649991,SRS22293060,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.9238,0.92347,0.08792,0.08777,0.75933,0.75921,0.56478,0.567,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33400,SRR30184306,SRX25649990,SRS22293063,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,cMO 3,wt cmo1 F,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:cMO 3|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L11,L11,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L11_S107_L004_R1_001.fastq.gz L11_S107_L004_R2_001.fastq.gz,fastq fastq,17297093488.0,57275144.0,L11 S107 L004 R1 001.fastq.gz,0:151 1:151,A:4727002988;C:3860128821;G:3837802284;T:4870157333;N:2002062,151,151,,,4727002988,3860128821,3837802284,4870157333,2002062,SRX25649990,SRS22293063,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.86746,0.86798,0.10604,0.1069,0.76396,0.7639,0.56148,0.55457,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33401,SRR30184307,SRX25649989,SRS22293058,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,uninjured 2,wt L2 M,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:uninjured 2|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L02,L02,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L02_S141_L006_R2_001.fastq.gz L02_S141_L006_R1_001.fastq.gz,fastq fastq,24399093702.0,80791701.0,L02 S141 L006 R1 001.fastq.gz,0:151 1:151,A:6567405598;C:5419524814;G:5626869131;T:6781638199;N:3655960,151,151,,,6567405598,5419524814,5626869131,6781638199,3655960,SRX25649989,SRS22293058,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.88324,0.88428,0.12546,0.12616,0.77141,0.77021,0.58483,0.58455,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System 33402,SRR30184308,SRX25649988,SRS22293057,SRP525334,PRJNA1145997,Regulation of optic nerve regeneration by srebf2,PRJNA1145997,Other,Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control nonspecific morpholino control morpholino mediated srebf2 gene knockdown nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.,,,,uninjured 1,wt L1 M,,strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman Medical College of Wisconsin|treatment:uninjured 1|BioSampleModel:Model organism or animal,,,,,,,,,LCM seq of Danio rerio:retinal ganglion cell layer,L01,L01,LCM seq NucleoSpin RNA XS purified ultra low input RNAseq at Novogene,,,RNA-Seq,TRANSCRIPTOMIC,cDNA_oligo_dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP525334,,,L01_S123_L005_R2_001.fastq.gz L01_S123_L005_R1_001.fastq.gz,fastq fastq,17596481188.0,58266494.0,L01 S123 L005 R1 001.fastq.gz,0:151 1:151,A:4773806012;C:3941529807;G:3951952531;T:4927955026;N:1237812,151,151,,,4773806012,3941529807,3951952531,4927955026,1237812,SRX25649988,SRS22293057,SRA1943640,"Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy",Medical College of Wisconsin,2,0.9124,0.91249,0.10052,0.10034,0.76751,0.76773,0.58532,0.58581,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2024-08-09,Adult,Adult,Eye,Sensory System