rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
14,DRR334977,DRX323973,DRS217313,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 28 degrees rep 3,SAMD00422597,,sample name:rRNA mock community 28 degrees rep 3|biological replicate:3|collection date:2021 01 15|dev stage:Adult|technical replicate:3|temp:28|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422597,DRX323973,t28 3 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422597,,,,3791170746.0,37701921.0,DRR334977,0:100.56 1:0,A:967477491;C:926887571;G:898697642;T:998107868;N:174,100,0,,,967477491,926887571,898697642,998107868,174,DRX323973,DRS217313,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.31527,,0.06446,,0.88844,,0.62118,,101,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
15,DRR334976,DRX323972,DRS217312,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 28 degrees rep 2,SAMD00422596,,sample name:rRNA mock community 28 degrees rep 2|biological replicate:3|collection date:2021 01 15|dev stage:Adult|technical replicate:2|temp:28|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422596,DRX323972,t28 2 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422596,,,,2801693695.0,27860658.0,DRR334976,0:100.56 1:0,A:700189496;C:702936549;G:679920984;T:718646067;N:599,100,0,,,700189496,702936549,679920984,718646067,599,DRX323972,DRS217312,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.43508,,0.08819,,0.85859,,0.69447,,101,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
16,DRR334975,DRX323971,DRS217311,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 28 degrees rep 1,SAMD00422595,,sample name:rRNA mock community 28 degrees rep 1|biological replicate:3|collection date:2021 01 15|dev stage:Adult|technical replicate:1|temp:28|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422595,DRX323971,t28 1 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422595,,,,3148691934.0,31307464.0,DRR334975,0:100.57 1:0,A:785000734;C:791315678;G:766594599;T:805780465;N:458,100,0,,,785000734,791315678,766594599,805780465,458,DRX323971,DRS217311,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.37139,,0.09061,,0.94065,,0.74047,,101,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
17,DRR334974,DRX323970,DRS217310,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 19 degrees rep 3,SAMD00422594,,sample name:rRNA mock community 19 degrees rep 3|biological replicate:2|collection date:2021 01 15|dev stage:Adult|technical replicate:3|temp:19|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422594,DRX323970,t19 3 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422594,,,,2856186273.0,28400524.0,DRR334974,0:100.57 1:0,A:695685787;C:733754277;G:715270279;T:711475544;N:386,100,0,,,695685787,733754277,715270279,711475544,386,DRX323970,DRS217310,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.48152,,0.10933,,0.87105,,0.7287,,101,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
18,DRR334973,DRX323969,DRS217309,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 19 degrees rep 2,SAMD00422593,,sample name:rRNA mock community 19 degrees rep 2|biological replicate:2|collection date:2021 01 15|dev stage:Adult|technical replicate:2|temp:19|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422593,DRX323969,t19 2 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422593,,,,3199929804.0,31816971.0,DRR334973,0:100.57 1:0,A:777149590;C:825478743;G:804835148;T:792466124;N:199,100,0,,,777149590,825478743,804835148,792466124,199,DRX323969,DRS217309,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.4418,,0.10282,,0.89706,,0.74667,,101,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
19,DRR334972,DRX323968,DRS217308,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 19 degrees rep 1,SAMD00422592,,sample name:rRNA mock community 19 degrees rep 1|biological replicate:2|collection date:2021 01 15|dev stage:Adult|technical replicate:1|temp:19|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422592,DRX323968,t19 1 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422592,,,,3658675391.0,36374718.0,DRR334972,0:100.58 1:0,A:879342063;C:954687406;G:929537615;T:895107890;N:417,100,0,,,879342063,954687406,929537615,895107890,417,DRX323968,DRS217308,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.49439,,0.11691,,0.88239,,0.73925,,101,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
20,DRR334971,DRX323967,DRS217307,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 10 degrees rep 3,SAMD00422591,,sample name:rRNA mock community 10 degrees rep 3|biological replicate:1|collection date:2021 01 15|dev stage:Adult|technical replicate:3|temp:10|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422591,DRX323967,t10 3 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422591,,,,3017524690.0,30006334.0,DRR334971,0:100.56 1:0,A:771967717;C:738814269;G:712866126;T:793876235;N:343,100,0,,,771967717,738814269,712866126,793876235,343,DRX323967,DRS217307,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.33157,,0.07363,,0.9093,,0.73422,,100,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
21,DRR334970,DRX323966,DRS217306,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 10 degrees rep 2,SAMD00422590,,sample name:rRNA mock community 10 degrees rep 2|biological replicate:1|collection date:2021 01 15|dev stage:Adult|technical replicate:2|temp:10|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422590,DRX323966,t10 2 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422590,,,,3115915184.0,30982336.0,DRR334970,0:100.57 1:0,A:765727966;C:794741415;G:771082024;T:784363609;N:170,100,0,,,765727966,794741415,771082024,784363609,170,DRX323966,DRS217306,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.45458,,0.10504,,0.89357,,0.749,,100,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
22,DRR334969,DRX323965,DRS217305,DRP008001,PRJDB12578,Allometric scaling of RNA abundance from genes to communities,DRP008001,Other,The metabolic theory of ecology MTE and growth rate hypothesis GRH help explain the mechanistic basis of size allometry and temperature dependence on growth rate and whole  body RNA content in organisms. However  testing RNA allometric scaling with next generation sequencing is yet to be done. Here  we validated the assumptions of GRH and MTE on messenger RNA and ribosome abundance using mock community metatranscriptome analysis. Our findings highlight that fast growing smaller species harbor greater RNA abundance per mass of tissue compared with species having larger body sizes and slower growth rates. We found that genome size and body size impose significant constraints in interspecific RNA abundance scaling  while the assumed temperature dependence appeared to be weak. Lastly  allometric scaling integration in community level models may extend the use of metatranscriptomics as a reliable tool for estimating ecosystem processes.,,,totalRNA metatranscriptomic sequences from mock communities consist of five model species,rRNA mock community at 10 degrees rep 1,SAMD00422589,,sample name:rRNA mock community 10 degrees rep 1|biological replicate:1|collection date:2021 01 15|dev stage:Adult|technical replicate:1|temp:10|treatment:rRNA,,,,,,,,,NextSeq 2000 sequencing of SAMD00422589,DRX323965,t10 1 tRNA.fastq,1,NEBNext Kit for Illumina,,RNA-Seq,METATRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP008001,NextSeq 2000 sequencing of SAMD00422589,,,,3206657309.0,31883532.0,DRR334969,0:100.57 1:0,A:792874386;C:814578518;G:787469614;T:811734581;N:210,100,0,,,792874386,814578518,787469614,811734581,210,DRX323965,DRS217305,DRA013226,"SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica","SINICA|Machida Laboratory Biodiversity Research Center, Academia Sinica",1,0.40156,,0.09664,,0.92898,,0.74114,,101,,B,,usable mapping rate,illumina,nextseq_v2,unknown,random_priming,nebnext,bulk,unknown,unknown,,Taiwan,2021-12-23,Adult,Adult,Undetermined,Undetermined
14875,ERR12071836,ERX11454461,ERS16387703,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 1,SAMEA114399011,University of East Anglia,ENA FIRST PUBLIC:2023 10 06T08:31:44Z|ENA LAST UPDATE:2023 10 06T08:31:44Z|External Id:SAMEA114399011|INSDC center name:University of East Anglia|INSDC first public:2023 10 06T08:31:44Z|INSDC last update:2023 10 06T08:31:44Z|INSDC status:public|Submitter Id:115 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66715,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 10 06|ENA LAST UPDATE:2023 10 06,115-1_S69_L006_R1_001.fastq.gz 115-1_S69_L006_R2_001.fastq.gz,fastq fastq,12813280704.0,50846352.0,ena RUN TAB 21 09 2023 13:32:15:295 66716,0:126 1:126,A:2451295493;C:3891843753;G:4001866488;T:2448421880;N:19853090,126,126,,,2451295493,3891843753,4001866488,2448421880,19853090,ERX11454461,ERS16387703,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89907,0.8514,0.17845,0.173,0.80369,0.81085,0.83252,0.83449,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14876,ERR12071855,ERX11454480,ERS16387722,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 5,SAMEA114399030,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:300 66753,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-5_S40_L008_R1_001.fastq.gz 83-5_S40_L008_R2_001.fastq.gz,fastq fastq,13683493656.0,54299578.0,ena RUN TAB 21 09 2023 13:32:15:300 66754,0:126 1:126,A:2772843483;C:4073415925;G:4192640412;T:2641952920;N:2640916,126,126,,,2772843483,4073415925,4192640412,2641952920,2640916,ERX11454480,ERS16387722,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92452,0.92427,0.15846,0.16251,0.7777,0.77666,0.78362,0.77515,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14877,ERR12071854,ERX11454479,ERS16387721,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 4,SAMEA114399029,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66751,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-4_S8_L002_R1_001.fastq.gz 83-4_S8_L002_R2_001.fastq.gz,fastq fastq,10045714644.0,39863947.0,ena RUN TAB 21 09 2023 13:32:15:300 66752,0:126 1:126,A:2037388456;C:2963530056;G:3069663461;T:1972981871;N:2150800,126,126,,,2037388456,2963530056,3069663461,1972981871,2150800,ERX11454479,ERS16387721,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91872,0.91762,0.16049,0.16307,0.76516,0.76644,0.77023,0.77199,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14878,ERR12071853,ERX11454478,ERS16387720,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 3,SAMEA114399028,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66749,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-3_S12_L003_R1_001.fastq.gz 83-3_S12_L003_R2_001.fastq.gz,fastq fastq,17333567748.0,68783999.0,ena RUN TAB 21 09 2023 13:32:15:299 66750,0:126 1:126,A:3399006637;C:5245167754;G:5416988818;T:3269621086;N:2783453,126,126,,,3399006637,5245167754,5416988818,3269621086,2783453,ERX11454478,ERS16387720,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93044,0.93099,0.14459,0.1479,0.77287,0.774,0.78248,0.78348,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14879,ERR12071852,ERX11454477,ERS16387719,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 1,SAMEA114399027,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66747,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-1_S4_L001_R1_001.fastq.gz 83-1_S4_L001_R2_001.fastq.gz,fastq fastq,14977164132.0,59433191.0,ena RUN TAB 21 09 2023 13:32:15:299 66748,0:126 1:126,A:3115980365;C:4357431505;G:4531888641;T:2968758582;N:3105039,126,126,,,3115980365,4357431505,4531888641,2968758582,3105039,ERX11454477,ERS16387719,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89622,0.89549,0.15698,0.16074,0.76558,0.76779,0.70301,0.73129,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14880,ERR12071851,ERX11454476,ERS16387718,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 5,SAMEA114399026,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66745,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-5_S3_L001_R1_001.fastq.gz 82-5_S3_L001_R2_001.fastq.gz,fastq fastq,14575744764.0,57840257.0,ena RUN TAB 21 09 2023 13:32:15:299 66746,0:126 1:126,A:3079286238;C:4161280385;G:4315514217;T:3016549159;N:3114765,126,126,,,3079286238,4161280385,4315514217,3016549159,3114765,ERX11454476,ERS16387718,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.82553,0.82489,0.15941,0.16124,0.77337,0.77518,0.73773,0.75718,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14881,ERR12071850,ERX11454475,ERS16387717,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 3,SAMEA114399025,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66743,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-3_S11_L003_R1_001.fastq.gz 82-3_S11_L003_R2_001.fastq.gz,fastq fastq,15753924144.0,62515572.0,ena RUN TAB 21 09 2023 13:32:15:299 66744,0:126 1:126,A:3294606329;C:4538635184;G:4708756438;T:3209425450;N:2500743,126,126,,,3294606329,4538635184,4708756438,3209425450,2500743,ERX11454475,ERS16387717,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.8642,0.86388,0.1596,0.16298,0.77051,0.77297,0.7599,0.7352,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14882,ERR12071849,ERX11454474,ERS16387716,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 2,SAMEA114399024,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66741,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 22,82-2_S7_L002_R1_001.fastq.gz 82-2_S7_L002_R2_001.fastq.gz,fastq fastq,20680686684.0,82066217.0,ena RUN TAB 21 09 2023 13:32:15:298 66742,0:126 1:126,A:4358995918;C:5919331822;G:6101024757;T:4296885228;N:4448959,126,126,,,4358995918,5919331822,6101024757,4296885228,4448959,ERX11454474,ERS16387716,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.88722,0.88591,0.1721,0.1755,0.76207,0.76394,0.74661,0.7483,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14883,ERR12071848,ERX11454473,ERS16387715,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 1,SAMEA114399023,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66739,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-1_S39_L008_R1_001.fastq.gz 82-1_S39_L008_R2_001.fastq.gz,fastq fastq,19062862812.0,75646281.0,ena RUN TAB 21 09 2023 13:32:15:298 66740,0:126 1:126,A:4015412618;C:5485657253;G:5643544062;T:3914646370;N:3602509,126,126,,,4015412618,5485657253,5643544062,3914646370,3602509,ERX11454473,ERS16387715,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.82331,0.82289,0.14959,0.15262,0.77786,0.77368,0.75014,0.71846,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14884,ERR12071847,ERX11454472,ERS16387714,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 5,SAMEA114399022,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66737,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-5_S6_L002_R1_001.fastq.gz 81-5_S6_L002_R2_001.fastq.gz,fastq fastq,14105505960.0,55974230.0,ena RUN TAB 21 09 2023 13:32:15:298 66738,0:126 1:126,A:2867593807;C:4154280778;G:4295901883;T:2784713422;N:3016070,126,126,,,2867593807,4154280778,4295901883,2784713422,3016070,ERX11454472,ERS16387714,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89055,0.89059,0.15225,0.15462,0.77031,0.7709,0.77204,0.74565,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14885,ERR12071846,ERX11454471,ERS16387713,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 4,SAMEA114399021,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66735,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-4_S38_L008_R1_001.fastq.gz 81-4_S38_L008_R2_001.fastq.gz,fastq fastq,14056344540.0,55779145.0,ena RUN TAB 21 09 2023 13:32:15:298 66736,0:126 1:126,A:2941182079;C:4065026103;G:4178339299;T:2869101906;N:2695153,126,126,,,2941182079,4065026103,4178339299,2869101906,2695153,ERX11454471,ERS16387713,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.85089,0.84987,0.16263,0.16502,0.77837,0.77283,0.74931,0.72487,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14886,ERR12071845,ERX11454470,ERS16387712,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 2,SAMEA114399020,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66733,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-2_S10_L003_R2_001.fastq.gz 81-2_S10_L003_R1_001.fastq.gz,fastq fastq,12344567004.0,48986377.0,ena RUN TAB 21 09 2023 13:32:15:297 66734,0:126 1:126,A:2564870489;C:3577715066;G:3699995887;T:2500054422;N:1931140,126,126,,,2564870489,3577715066,3699995887,2500054422,1931140,ERX11454470,ERS16387712,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.81332,0.81278,0.14345,0.14595,0.77962,0.78011,0.77642,0.76714,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14887,ERR12071844,ERX11454469,ERS16387711,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 1,SAMEA114399019,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66731,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-1_S2_L001_R1_001.fastq.gz 81-1_S2_L001_R2_001.fastq.gz,fastq fastq,16863847056.0,66920028.0,ena RUN TAB 21 09 2023 13:32:15:297 66732,0:126 1:126,A:3484102096;C:4908704805;G:5083802657;T:3383595635;N:3641863,126,126,,,3484102096,4908704805,5083802657,3383595635,3641863,ERX11454469,ERS16387711,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90019,0.89959,0.16463,0.16683,0.76607,0.76792,0.76365,0.73132,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14888,ERR12071843,ERX11454468,ERS16387710,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 4,SAMEA114399018,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66729,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-4_S9_L003_R1_001.fastq.gz 80-4_S9_L003_R2_001.fastq.gz,fastq fastq,22230539604.0,88216427.0,ena RUN TAB 21 09 2023 13:32:15:297 66730,0:126 1:126,A:4399600032;C:6651638899;G:6855034908;T:4320719496;N:3546269,126,126,,,4399600032,6651638899,6855034908,4320719496,3546269,ERX11454468,ERS16387710,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84585,0.84448,0.14327,0.14586,0.79078,0.79251,0.79803,0.79459,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14889,ERR12071842,ERX11454467,ERS16387709,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 3,SAMEA114399017,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66727,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-3_S1_L001_R2_001.fastq.gz 80-3_S1_L001_R1_001.fastq.gz,fastq fastq,17944230024.0,71207262.0,ena RUN TAB 21 09 2023 13:32:15:296 66728,0:126 1:126,A:3713490828;C:5207225554;G:5378396527;T:3641393155;N:3723960,126,126,,,3713490828,5207225554,5378396527,3641393155,3723960,ERX11454467,ERS16387709,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84454,0.84386,0.14922,0.15129,0.77264,0.77339,0.75797,0.76206,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14890,ERR12071841,ERX11454466,ERS16387708,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 2,SAMEA114399016,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66725,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-2_S5_L002_R1_001.fastq.gz 80-2_S5_L002_R2_001.fastq.gz,fastq fastq,16641238320.0,66036660.0,ena RUN TAB 21 09 2023 13:32:15:296 66726,0:126 1:126,A:3508280857;C:4757668643;G:4898009962;T:3473768461;N:3510397,126,126,,,3508280857,4757668643,4898009962,3473768461,3510397,ERX11454466,ERS16387708,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.86106,0.86111,0.16002,0.16307,0.7653,0.76542,0.72623,0.70444,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14891,ERR12071840,ERX11454465,ERS16387707,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 1,SAMEA114399015,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66723,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-1_S37_L008_R1_001.fastq.gz 80-1_S37_L008_R2_001.fastq.gz,fastq fastq,15269215248.0,60592124.0,ena RUN TAB 21 09 2023 13:32:15:296 66724,0:126 1:126,A:3161128702;C:4439474080;G:4571245477;T:3094451760;N:2915229,126,126,,,3161128702,4439474080,4571245477,3094451760,2915229,ERX11454465,ERS16387707,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84008,0.83924,0.16203,0.16482,0.78486,0.78301,0.76847,0.76451,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14892,ERR12071839,ERX11454464,ERS16387706,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 5,SAMEA114399014,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66721,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-5_S28_L005_R2_001.fastq.gz 115-5_S28_L005_R1_001.fastq.gz,fastq fastq,13704997824.0,54384912.0,ena RUN TAB 21 09 2023 13:32:15:296 66722,0:126 1:126,A:2791048995;C:4040751875;G:4161655954;T:2708992373;N:2548627,126,126,,,2791048995,4040751875,4161655954,2708992373,2548627,ERX11454464,ERS16387706,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92152,0.922,0.16432,0.16746,0.77977,0.77723,0.7658,0.74319,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14893,ERR12071838,ERX11454463,ERS16387705,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 4,SAMEA114399013,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66719,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-4_S73_L007_R1_001.fastq.gz 115-4_S73_L007_R2_001.fastq.gz,fastq fastq,13555374840.0,53791170.0,ena RUN TAB 21 09 2023 13:32:15:295 66720,0:126 1:126,A:2613879041;C:4131073531;G:4251239682;T:2536011431;N:23171155,126,126,,,2613879041,4131073531,4251239682,2536011431,23171155,ERX11454463,ERS16387705,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93971,0.93992,0.15435,0.15669,0.78766,0.79076,0.76218,0.78676,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14894,ERR12071837,ERX11454462,ERS16387704,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 2,SAMEA114399012,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66717,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-2_S77_L008_R1_001.fastq.gz 115-2_S77_L008_R2_001.fastq.gz,fastq fastq,19309168872.0,76623686.0,ena RUN TAB 21 09 2023 13:32:15:295 66718,0:126 1:126,A:3659403129;C:5914313547;G:6102556630;T:3596021103;N:36874463,126,126,,,3659403129,5914313547,6102556630,3596021103,36874463,ERX11454462,ERS16387704,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90206,0.90085,0.164,0.16686,0.81477,0.81856,0.84674,0.83786,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14895,ERR12071835,ERX11454460,ERS16387702,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 5,SAMEA114399010,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66713,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-5_S68_L006_R1_001.fastq.gz 114-5_S68_L006_R2_001.fastq.gz,fastq fastq,14161249872.0,56195436.0,ena RUN TAB 21 09 2023 13:32:15:295 66714,0:126 1:126,A:2927386834;C:4098238005;G:4213138034;T:2900333935;N:22153064,126,126,,,2927386834,4098238005,4213138034,2900333935,22153064,ERX11454460,ERS16387702,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92035,0.91983,0.16785,0.17009,0.75864,0.76292,0.73081,0.75429,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14896,ERR12071834,ERX11454459,ERS16387701,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 4,SAMEA114399009,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66711,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-4_S76_L008_R1_001.fastq.gz 114-4_S76_L008_R2_001.fastq.gz,fastq fastq,12939456600.0,51347050.0,ena RUN TAB 21 09 2023 13:32:15:294 66712,0:126 1:126,A:2686900916;C:3725092053;G:3826228928;T:2676529407;N:24705296,126,126,,,2686900916,3725092053,3826228928,2676529407,24705296,ERX11454459,ERS16387701,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9171,0.91541,0.19718,0.20005,0.76759,0.77285,0.75547,0.75357,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14897,ERR12071833,ERX11454458,ERS16387700,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 3,SAMEA114399008,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66709,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-3_S72_L007_R1_001.fastq.gz 114-3_S72_L007_R2_001.fastq.gz,fastq fastq,15510255012.0,61548631.0,ena RUN TAB 21 09 2023 13:32:15:294 66710,0:126 1:126,A:3024458012;C:4641826332;G:4764527525;T:3052903565;N:26539578,126,126,,,3024458012,4641826332,4764527525,3052903565,26539578,ERX11454458,ERS16387700,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.85112,0.84868,0.17054,0.17432,0.79547,0.79851,0.73756,0.8091,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14898,ERR12071832,ERX11454457,ERS16387699,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 1,SAMEA114399007,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66707,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-1_S27_L005_R1_001.fastq.gz 114-1_S27_L005_R2_001.fastq.gz,fastq fastq,15544755576.0,61685538.0,ena RUN TAB 21 09 2023 13:32:15:294 66708,0:126 1:126,A:3077913021;C:4638218386;G:4760672592;T:3065112184;N:2839393,126,126,,,3077913021,4638218386,4760672592,3065112184,2839393,ERX11454457,ERS16387699,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90822,0.90777,0.1775,0.18112,0.79263,0.78808,0.82214,0.79489,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14899,ERR12071831,ERX11454456,ERS16387698,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 9,SAMEA114399006,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 9|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 9|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66705,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-9_S67_L006_R1_001.fastq.gz 113-9_S67_L006_R2_001.fastq.gz,fastq fastq,15414133644.0,61167197.0,ena RUN TAB 21 09 2023 13:32:15:293 66706,0:126 1:126,A:3185120611;C:4467167719;G:4606569127;T:3131094561;N:24181626,126,126,,,3185120611,4467167719,4606569127,3131094561,24181626,ERX11454456,ERS16387698,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94176,0.94197,0.17489,0.17743,0.76063,0.76295,0.75218,0.75546,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14900,ERR12071830,ERX11454455,ERS16387697,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 13,SAMEA114399005,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 13|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 13|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66703,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-13_S71_L007_R1_001.fastq.gz 113-13_S71_L007_R2_001.fastq.gz,fastq fastq,18125498160.0,71926580.0,ena RUN TAB 21 09 2023 13:32:15:293 66704,0:126 1:126,A:3681246224;C:5316613237;G:5486708206;T:3609735923;N:31194570,126,126,,,3681246224,5316613237,5486708206,3609735923,31194570,ERX11454455,ERS16387697,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93952,0.93814,0.1717,0.17395,0.76627,0.76976,0.76533,0.72702,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14901,ERR12071829,ERX11454454,ERS16387696,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 11,SAMEA114399004,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 11|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 11|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66701,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-11_S26_L005_R1_001.fastq.gz 113-11_S26_L005_R2_001.fastq.gz,fastq fastq,17918758872.0,71106186.0,ena RUN TAB 21 09 2023 13:32:15:293 66702,0:126 1:126,A:3460235614;C:5469489967;G:5622549540;T:3363214846;N:3268905,126,126,,,3460235614,5469489967,5622549540,3363214846,3268905,ERX11454454,ERS16387696,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94836,0.94773,0.16512,0.16708,0.79843,0.79251,0.79716,0.79211,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14902,ERR12071828,ERX11454453,ERS16387695,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 10,SAMEA114399003,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 10|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 10|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66699,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-10_S75_L008_R1_001.fastq.gz 113-10_S75_L008_R2_001.fastq.gz,fastq fastq,22137738588.0,87848169.0,ena RUN TAB 21 09 2023 13:32:15:293 66700,0:126 1:126,A:4240961737;C:6714114283;G:6924397580;T:4216314997;N:41949991,126,126,,,4240961737,6714114283,6924397580,4216314997,41949991,ERX11454453,ERS16387695,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94101,0.93949,0.15969,0.16256,0.782,0.78429,0.78877,0.78359,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14903,ERR12071827,ERX11454452,ERS16387694,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 8,SAMEA114399002,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 8|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 8|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66697,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-8_S74_L008_R1_001.fastq.gz 112-8_S74_L008_R2_001.fastq.gz,fastq fastq,15579825912.0,61824706.0,ena RUN TAB 21 09 2023 13:32:15:292 66698,0:126 1:126,A:3262384535;C:4470619467;G:4585527202;T:3231451715;N:29842993,126,126,,,3262384535,4470619467,4585527202,3231451715,29842993,ERX11454452,ERS16387694,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92603,0.92484,0.18249,0.18589,0.75917,0.76335,0.73196,0.72728,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14904,ERR12071826,ERX11454451,ERS16387693,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 7,SAMEA114399001,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 7|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 7|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66695,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-7_S66_L006_R1_001.fastq.gz 112-7_S66_L006_R2_001.fastq.gz,fastq fastq,20203529976.0,80172738.0,ena RUN TAB 21 09 2023 13:32:15:292 66696,0:126 1:126,A:4115948087;C:5902381830;G:6064166888;T:4089530757;N:31502414,126,126,,,4115948087,5902381830,6064166888,4089530757,31502414,ERX11454451,ERS16387693,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92896,0.92845,0.17083,0.17434,0.76146,0.76337,0.6945,0.74323,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14905,ERR12071825,ERX11454450,ERS16387692,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 5,SAMEA114399000,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66693,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-5_S70_L007_R1_001.fastq.gz 112-5_S70_L007_R2_001.fastq.gz,fastq fastq,14979433392.0,59442196.0,ena RUN TAB 21 09 2023 13:32:15:292 66694,0:126 1:126,A:2989976300;C:4427492967;G:4545641644;T:2990769657;N:25552824,126,126,,,2989976300,4427492967,4545641644,2990769657,25552824,ERX11454450,ERS16387692,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9139,0.91293,0.18796,0.1912,0.78443,0.78597,0.79005,0.77582,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14906,ERR12071824,ERX11454449,ERS16387691,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 4,SAMEA114398999,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66691,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-4_S25_L005_R1_001.fastq.gz 112-4_S25_L005_R2_001.fastq.gz,fastq fastq,14850028368.0,58928684.0,ena RUN TAB 21 09 2023 13:32:15:292 66692,0:126 1:126,A:3108605429;C:4289376091;G:4392727411;T:3056545411;N:2774026,126,126,,,3108605429,4289376091,4392727411,3056545411,2774026,ERX11454449,ERS16387691,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92451,0.92555,0.17853,0.18265,0.76501,0.76106,0.73074,0.72504,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14907,ERR12071823,ERX11454448,ERS16387690,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 4,SAMEA114398998,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66689,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-4_S8_L002_R1_001.fastq.gz 111-4_S8_L002_R2_001.fastq.gz,fastq fastq,17826911676.0,70741713.0,ena RUN TAB 21 09 2023 13:32:15:291 66690,0:126 1:126,A:3435687537;C:5460019012;G:5625529849;T:3303228205;N:2447073,126,126,,,3435687537,5460019012,5625529849,3303228205,2447073,ERX11454448,ERS16387690,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95917,0.95936,0.18841,0.18992,0.81367,0.81523,0.79429,0.81623,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14908,ERR12071822,ERX11454447,ERS16387689,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 3,SAMEA114398997,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66687,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-3_S16_L004_R1_001.fastq.gz 111-3_S16_L004_R2_001.fastq.gz,fastq fastq,16893915192.0,67039346.0,ena RUN TAB 21 09 2023 13:32:15:291 66688,0:126 1:126,A:3264486030;C:5167828698;G:5330646003;T:3128597235;N:2357226,126,126,,,3264486030,5167828698,5330646003,3128597235,2357226,ERX11454447,ERS16387689,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96382,0.96339,0.19876,0.20112,0.81893,0.82102,0.81987,0.79966,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14909,ERR12071821,ERX11454446,ERS16387688,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 2,SAMEA114398996,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66685,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-2_S12_L003_R1_001.fastq.gz 111-2_S12_L003_R2_001.fastq.gz,fastq fastq,19135485684.0,75934467.0,ena RUN TAB 21 09 2023 13:32:15:291 66686,0:126 1:126,A:3609533802;C:5946063742;G:6161038930;T:3416257009;N:2592201,126,126,,,3609533802,5946063742,6161038930,3416257009,2592201,ERX11454446,ERS16387688,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96183,0.96186,0.17579,0.17791,0.81972,0.82047,0.77379,0.82377,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14910,ERR12071820,ERX11454445,ERS16387687,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 1,SAMEA114398995,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66683,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-1_S4_L001_R1_001.fastq.gz 111-1_S4_L001_R2_001.fastq.gz,fastq fastq,21082514796.0,83660773.0,ena RUN TAB 21 09 2023 13:32:15:291 66684,0:126 1:126,A:4043020782;C:6480787651;G:6689150606;T:3865255761;N:4299996,126,126,,,4043020782,6480787651,6689150606,3865255761,4299996,ERX11454445,ERS16387687,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96289,0.963,0.18676,0.18828,0.81791,0.81988,0.78774,0.81813,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14911,ERR12071819,ERX11454444,ERS16387686,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 4,SAMEA114398994,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66681,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-4_S3_L001_R2_001.fastq.gz 110-4_S3_L001_R1_001.fastq.gz,fastq fastq,14687310204.0,58282977.0,ena RUN TAB 21 09 2023 13:32:15:290 66682,0:126 1:126,A:2926150199;C:4367282172;G:4510560380;T:2880182221;N:3135232,126,126,,,2926150199,4367282172,4510560380,2880182221,3135232,ERX11454444,ERS16387686,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93442,0.934,0.1706,0.17527,0.77611,0.7782,0.79075,0.77981,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14912,ERR12071818,ERX11454443,ERS16387685,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 3,SAMEA114398993,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66679,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-3_S11_L003_R2_001.fastq.gz 110-3_S11_L003_R1_001.fastq.gz,fastq fastq,13850712792.0,54963146.0,ena RUN TAB 21 09 2023 13:32:15:290 66680,0:126 1:126,A:2754372507;C:4139837776;G:4290528181;T:2664097645;N:1876683,126,126,,,2754372507,4139837776,4290528181,2664097645,1876683,ERX11454443,ERS16387685,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94187,0.94169,0.16695,0.17006,0.77824,0.78044,0.7861,0.7945,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14913,ERR12071817,ERX11454442,ERS16387684,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 2,SAMEA114398992,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66677,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-2_S15_L004_R2_001.fastq.gz 110-2_S15_L004_R1_001.fastq.gz,fastq fastq,15866607708.0,62962729.0,ena RUN TAB 21 09 2023 13:32:15:290 66678,0:126 1:126,A:3214891728;C:4688310800;G:4841314699;T:3119879854;N:2210627,126,126,,,3214891728,4688310800,4841314699,3119879854,2210627,ERX11454442,ERS16387684,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93647,0.93604,0.16699,0.17,0.76641,0.76909,0.76728,0.77117,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14914,ERR12071816,ERX11454441,ERS16387683,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 1,SAMEA114398991,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66675,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-1_S7_L002_R2_001.fastq.gz 110-1_S7_L002_R1_001.fastq.gz,fastq fastq,15365002212.0,60972231.0,ena RUN TAB 21 09 2023 13:32:15:290 66676,0:126 1:126,A:3114598527;C:4531528684;G:4684815069;T:3031977480;N:2082452,126,126,,,3114598527,4531528684,4684815069,3031977480,2082452,ERX11454441,ERS16387683,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93661,0.936,0.17032,0.17368,0.7699,0.77157,0.74201,0.77289,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14915,ERR12071815,ERX11454440,ERS16387682,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 4,SAMEA114398990,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66673,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-4_S14_L004_R1_001.fastq.gz 109-4_S14_L004_R2_001.fastq.gz,fastq fastq,17693786880.0,70213440.0,ena RUN TAB 21 09 2023 13:32:15:289 66674,,,,,,,,,,,,ERX11454440,ERS16387682,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93895,0.93626,0.13454,0.13567,0.80679,0.80906,0.83187,0.85713,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14916,ERR12071814,ERX11454439,ERS16387681,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 3,SAMEA114398989,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66671,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-3_S6_L002_R1_001.fastq.gz 109-3_S6_L002_R2_001.fastq.gz,fastq fastq,15326268300.0,60818525.0,ena RUN TAB 21 09 2023 13:32:15:289 66672,0:126 1:126,A:2963028716;C:4693248657;G:4853138006;T:2814772075;N:2080846,126,126,,,2963028716,4693248657,4853138006,2814772075,2080846,ERX11454439,ERS16387681,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95492,0.95516,0.18592,0.18766,0.816,0.81722,0.82037,0.77416,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14917,ERR12071813,ERX11454438,ERS16387680,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 2,SAMEA114398988,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66669,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-2_S9_L003_R1_001.fastq.gz 109-2_S9_L003_R2_001.fastq.gz,fastq fastq,13463300088.0,53425794.0,ena RUN TAB 21 09 2023 13:32:15:289 66670,0:126 1:126,A:2565791374;C:4152722401;G:4318203775;T:2424775489;N:1807049,126,126,,,2565791374,4152722401,4318203775,2424775489,1807049,ERX11454438,ERS16387680,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96145,0.9621,0.19448,0.19497,0.83382,0.83451,0.84292,0.79683,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14918,ERR12071812,ERX11454437,ERS16387679,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 1,SAMEA114398987,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66667,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-1_S1_L001_R2_001.fastq.gz 109-1_S1_L001_R1_001.fastq.gz,fastq fastq,16254330624.0,64501312.0,ena RUN TAB 21 09 2023 13:32:15:289 66668,0:126 1:126,A:3146898181;C:4962037311;G:5139473645;T:3002518241;N:3403246,126,126,,,3146898181,4962037311,5139473645,3002518241,3403246,ERX11454437,ERS16387679,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95901,0.9593,0.19067,0.19204,0.8174,0.81864,0.7987,0.8132,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14919,ERR12071811,ERX11454436,ERS16387678,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 4,SAMEA114398986,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66665,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-4_S10_L003_R1_001.fastq.gz 108-4_S10_L003_R2_001.fastq.gz,fastq fastq,16401717108.0,65086179.0,ena RUN TAB 21 09 2023 13:32:15:288 66666,0:126 1:126,A:3306091376;C:4834896468;G:4995683070;T:3262829888;N:2216306,126,126,,,3306091376,4834896468,4995683070,3262829888,2216306,ERX11454436,ERS16387678,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92612,0.92543,0.16352,0.16724,0.76607,0.76812,0.76693,0.75698,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14920,ERR12071810,ERX11454435,ERS16387677,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 3,SAMEA114398985,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66663,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-3_S2_L001_R2_001.fastq.gz 108-3_S2_L001_R1_001.fastq.gz,fastq fastq,14063114268.0,55806009.0,ena RUN TAB 21 09 2023 13:32:15:288 66664,0:126 1:126,A:2855140106;C:4126986255;G:4254731693;T:2823386830;N:2869384,126,126,,,2855140106,4126986255,4254731693,2823386830,2869384,ERX11454435,ERS16387677,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89956,0.89846,0.17394,0.17665,0.77695,0.77751,0.78333,0.77908,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14921,ERR12071809,ERX11454434,ERS16387676,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 2,SAMEA114398984,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66661,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-2_S13_L004_R1_001.fastq.gz 108-2_S13_L004_R2_001.fastq.gz,fastq fastq,17951370444.0,71235597.0,ena RUN TAB 21 09 2023 13:32:15:288 66662,0:126 1:126,A:3419406758;C:5496702371;G:5719743964;T:3312990750;N:2526601,126,126,,,3419406758,5496702371,5719743964,3312990750,2526601,ERX11454434,ERS16387676,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91308,0.91164,0.14161,0.14408,0.78725,0.78851,0.77166,0.81536,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14922,ERR12071808,ERX11454433,ERS16387675,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 1,SAMEA114398983,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66659,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-1_S5_L002_R1_001.fastq.gz 108-1_S5_L002_R2_001.fastq.gz,fastq fastq,17592295140.0,69810695.0,ena RUN TAB 21 09 2023 13:32:15:288 66660,0:126 1:126,A:3473054128;C:5265562453;G:5455395791;T:3395881406;N:2401362,126,126,,,3473054128,5265562453,5455395791,3395881406,2401362,ERX11454433,ERS16387675,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92632,0.92464,0.16285,0.16525,0.7781,0.77985,0.79539,0.79841,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14923,ERR12071807,ERX11454432,ERS16387674,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 4,SAMEA114398982,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66657,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-4_S20_L005_R1_001.fastq.gz 107-4_S20_L005_R2_001.fastq.gz,fastq fastq,12626647992.0,50105746.0,ena RUN TAB 21 09 2023 13:32:15:287 66658,0:126 1:126,A:2503116695;C:3776878144;G:3904732537;T:2438634661;N:3285955,126,126,,,2503116695,3776878144,3904732537,2438634661,3285955,ERX11454432,ERS16387674,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93409,0.93399,0.16467,0.16803,0.77567,0.77749,0.77962,0.76236,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14924,ERR12071806,ERX11454431,ERS16387673,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 3,SAMEA114398981,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66655,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-3_S28_L007_R1_001.fastq.gz 107-3_S28_L007_R2_001.fastq.gz,fastq fastq,19765610928.0,78434964.0,ena RUN TAB 21 09 2023 13:32:15:287 66656,0:126 1:126,A:3807913538;C:6041902918;G:6270499946;T:3639644996;N:5649530,126,126,,,3807913538,6041902918,6270499946,3639644996,5649530,ERX11454431,ERS16387673,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93479,0.93431,0.15154,0.15299,0.78462,0.78533,0.78429,0.79385,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14925,ERR12071805,ERX11454430,ERS16387672,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 2,SAMEA114398980,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66653,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-2_S32_L008_R1_001.fastq.gz 107-2_S32_L008_R2_001.fastq.gz,fastq fastq,20245786344.0,80340422.0,ena RUN TAB 21 09 2023 13:32:15:287 66654,0:126 1:126,A:3580212812;C:6483019868;G:6732117106;T:3443314781;N:7121777,126,126,,,3580212812,6483019868,6732117106,3443314781,7121777,ERX11454430,ERS16387672,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93526,0.93431,0.14042,0.14264,0.82887,0.83041,0.85552,0.85524,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14926,ERR12071804,ERX11454429,ERS16387671,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 1,SAMEA114398979,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66651,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-1_S24_L006_R2_001.fastq.gz 107-1_S24_L006_R1_001.fastq.gz,fastq fastq,15192276120.0,60286810.0,ena RUN TAB 21 09 2023 13:32:15:286 66652,0:126 1:126,A:2840014487;C:4709824279;G:4867364305;T:2771588673;N:3484376,126,126,,,2840014487,4709824279,4867364305,2771588673,3484376,ERX11454429,ERS16387671,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92039,0.91914,0.17007,0.17237,0.80939,0.80986,0.84885,0.847,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14927,ERR12071803,ERX11454428,ERS16387670,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 4,SAMEA114398978,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66649,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-4_S23_L006_R2_001.fastq.gz 106-4_S23_L006_R1_001.fastq.gz,fastq fastq,16985562300.0,67403025.0,ena RUN TAB 21 09 2023 13:32:15:286 66650,0:126 1:126,A:3322275260;C:5133904780;G:5334495158;T:3190965461;N:3921641,126,126,,,3322275260,5133904780,5334495158,3190965461,3921641,ERX11454428,ERS16387670,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93707,0.93661,0.15576,0.15861,0.77826,0.78025,0.77722,0.77979,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14928,ERR12071802,ERX11454427,ERS16387669,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 3,SAMEA114398977,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66647,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-3_S31_L008_R2_001.fastq.gz 106-3_S31_L008_R1_001.fastq.gz,fastq fastq,19459285524.0,77219387.0,ena RUN TAB 21 09 2023 13:32:15:286 66648,0:126 1:126,A:3823996295;C:5872737349;G:6122836793;T:3632881865;N:6833222,126,126,,,3823996295,5872737349,6122836793,3632881865,6833222,ERX11454427,ERS16387669,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90902,0.90816,0.1544,0.15713,0.78926,0.79176,0.76867,0.78913,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14929,ERR12071801,ERX11454426,ERS16387668,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 2,SAMEA114398976,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66645,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-2_S27_L007_R1_001.fastq.gz 106-2_S27_L007_R2_001.fastq.gz,fastq fastq,15694418124.0,62279437.0,ena RUN TAB 21 09 2023 13:32:15:286 66646,0:126 1:126,A:3002717019;C:4829058190;G:5037608362;T:2820533482;N:4501071,126,126,,,3002717019,4829058190,5037608362,2820533482,4501071,ERX11454426,ERS16387668,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93511,0.93528,0.14004,0.14226,0.7891,0.79068,0.77063,0.77891,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14930,ERR12071800,ERX11454425,ERS16387667,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 1,SAMEA114398975,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66643,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-1_S19_L005_R1_001.fastq.gz 106-1_S19_L005_R2_001.fastq.gz,fastq fastq,18647636868.0,73998559.0,ena RUN TAB 21 09 2023 13:32:15:285 66644,0:126 1:126,A:3692341453;C:5587227660;G:5803402834;T:3559657331;N:5007590,126,126,,,3692341453,5587227660,5803402834,3559657331,5007590,ERX11454425,ERS16387667,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92714,0.92688,0.16214,0.16642,0.77433,0.775,0.76217,0.75322,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14931,ERR12071799,ERX11454424,ERS16387666,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 4,SAMEA114398974,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66641,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-4_S26_L007_R2_001.fastq.gz 105-4_S26_L007_R1_001.fastq.gz,fastq fastq,15124522392.0,60017946.0,ena RUN TAB 21 09 2023 13:32:15:285 66642,0:126 1:126,A:2852895871;C:4689063320;G:4867825391;T:2710443233;N:4294577,126,126,,,2852895871,4689063320,4867825391,2710443233,4294577,ERX11454424,ERS16387666,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95529,0.95479,0.13148,0.13281,0.7793,0.78125,0.78337,0.78508,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14932,ERR12071798,ERX11454423,ERS16387665,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 3,SAMEA114398973,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66639,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-3_S18_L005_R1_001.fastq.gz 105-3_S18_L005_R2_001.fastq.gz,fastq fastq,17644367664.0,70017332.0,ena RUN TAB 21 09 2023 13:32:15:285 66640,0:126 1:126,A:3347478814;C:5457952160;G:5642901286;T:3191260656;N:4774748,126,126,,,3347478814,5457952160,5642901286,3191260656,4774748,ERX11454423,ERS16387665,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94866,0.94893,0.14303,0.14517,0.78658,0.78697,0.80916,0.81131,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14933,ERR12071797,ERX11454422,ERS16387664,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 2,SAMEA114398972,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66637,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-2_S30_L008_R1_001.fastq.gz 105-2_S30_L008_R2_001.fastq.gz,fastq fastq,15981084252.0,63417001.0,ena RUN TAB 21 09 2023 13:32:15:285 66638,0:126 1:126,A:3059876138;C:4898463877;G:5076925794;T:2940239108;N:5579335,126,126,,,3059876138,4898463877,5076925794,2940239108,5579335,ERX11454422,ERS16387664,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95109,0.95022,0.14624,0.14776,0.78066,0.78283,0.76738,0.79238,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14934,ERR12071796,ERX11454421,ERS16387663,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 1,SAMEA114398971,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66635,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-1_S22_L006_R1_001.fastq.gz 105-1_S22_L006_R2_001.fastq.gz,fastq fastq,16378597368.0,64994434.0,ena RUN TAB 21 09 2023 13:32:15:284 66636,0:126 1:126,A:3157768088;C:4992606167;G:5190605482;T:3033853059;N:3764572,126,126,,,3157768088,4992606167,5190605482,3033853059,3764572,ERX11454421,ERS16387663,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9472,0.94619,0.15768,0.15997,0.78646,0.78845,0.75864,0.79159,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14935,ERR12071795,ERX11454420,ERS16387662,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 4,SAMEA114398970,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66633,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-4_S29_L008_R2_001.fastq.gz 104-4_S29_L008_R1_001.fastq.gz,fastq fastq,17713234476.0,70290613.0,ena RUN TAB 21 09 2023 13:32:15:284 66634,0:126 1:126,A:3387663411;C:5419734351;G:5646953646;T:3252634316;N:6248752,126,126,,,3387663411,5419734351,5646953646,3252634316,6248752,ERX11454420,ERS16387662,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91957,0.91797,0.14938,0.15201,0.79149,0.79358,0.7508,0.80456,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14936,ERR12071794,ERX11454419,ERS16387661,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 3,SAMEA114398969,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66631,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-3_S21_L006_R1_001.fastq.gz 104-3_S21_L006_R2_001.fastq.gz,fastq fastq,20412904428.0,81003589.0,ena RUN TAB 21 09 2023 13:32:15:284 66632,0:126 1:126,A:3999139733;C:6180998229;G:6441889554;T:3786215014;N:4661898,126,126,,,3999139733,6180998229,6441889554,3786215014,4661898,ERX11454419,ERS16387661,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93627,0.93494,0.14845,0.14979,0.77796,0.77891,0.76434,0.77202,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14937,ERR12071793,ERX11454418,ERS16387660,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 2,SAMEA114398968,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66629,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-2_S25_L007_R1_001.fastq.gz 104-2_S25_L007_R2_001.fastq.gz,fastq fastq,15067973340.0,59793545.0,ena RUN TAB 21 09 2023 13:32:15:284 66630,0:126 1:126,A:2963040605;C:4540311477;G:4736958045;T:2823360579;N:4302634,126,126,,,2963040605,4540311477,4736958045,2823360579,4302634,ERX11454418,ERS16387660,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92968,0.92854,0.16053,0.16248,0.78224,0.78228,0.7365,0.76845,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14938,ERR12071792,ERX11454417,ERS16387659,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 1,SAMEA114398967,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66627,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-1_S17_L005_R1_001.fastq.gz 104-1_S17_L005_R2_001.fastq.gz,fastq fastq,19257494508.0,76418629.0,ena RUN TAB 21 09 2023 13:32:15:283 66628,0:126 1:126,A:3731156408;C:5859411051;G:6100994986;T:3560909583;N:5022480,126,126,,,3731156408,5859411051,6100994986,3560909583,5022480,ERX11454417,ERS16387659,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92633,0.92586,0.14768,0.14948,0.78277,0.7833,0.7452,0.76737,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14939,ERR12071791,ERX11454416,ERS16387658,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 4,SAMEA114398966,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66625,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-4_S32_L006_R1_001.fastq.gz 103-4_S32_L006_R2_001.fastq.gz,fastq fastq,13085486820.0,51926535.0,ena RUN TAB 21 09 2023 13:32:15:283 66626,0:126 1:126,A:2567103783;C:3969617611;G:4069486176;T:2476759371;N:2519879,126,126,,,2567103783,3969617611,4069486176,2476759371,2519879,ERX11454416,ERS16387658,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93075,0.93242,0.14151,0.14331,0.774,0.76822,0.75401,0.76454,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14940,ERR12071790,ERX11454415,ERS16387657,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 3,SAMEA114398965,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66623,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-3_S65_L007_R2_001.fastq.gz 103-3_S65_L007_R1_001.fastq.gz,fastq fastq,11192352696.0,44414098.0,ena RUN TAB 21 09 2023 13:32:15:283 66624,0:126 1:126,A:2123359510;C:3455439467;G:3570545494;T:2039990322;N:3017903,126,126,,,2123359510,3455439467,3570545494,2039990322,3017903,ERX11454415,ERS16387657,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89542,0.89591,0.1351,0.13661,0.78948,0.79003,0.77414,0.80393,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14941,ERR12071789,ERX11454414,ERS16387656,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 2,SAMEA114398964,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66621,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-2_S36_L007_R1_001.fastq.gz 103-2_S36_L007_R2_001.fastq.gz,fastq fastq,10568377512.0,41938006.0,ena RUN TAB 21 09 2023 13:32:15:283 66622,0:126 1:126,A:2109516452;C:3179785410;G:3272667938;T:2004453838;N:1953874,126,126,,,2109516452,3179785410,3272667938,2004453838,1953874,ERX11454414,ERS16387656,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92729,0.92817,0.15094,0.1528,0.7685,0.76516,0.74143,0.74277,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14942,ERR12071788,ERX11454413,ERS16387655,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 1,SAMEA114398963,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66619,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-1_S69_L008_R1_001.fastq.gz 103-1_S69_L008_R2_001.fastq.gz,fastq fastq,9417798180.0,37372215.0,ena RUN TAB 21 09 2023 13:32:15:282 66620,0:126 1:126,A:1807793384;C:2891759578;G:2983948236;T:1730405095;N:3891887,126,126,,,1807793384,2891759578,2983948236,1730405095,3891887,ERX11454413,ERS16387655,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.8784,0.87802,0.13342,0.13445,0.789,0.78946,0.79547,0.79885,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14943,ERR12071787,ERX11454412,ERS16387654,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 4,SAMEA114398962,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66617,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-4_S35_L007_R2_001.fastq.gz 102-4_S35_L007_R1_001.fastq.gz,fastq fastq,15605254728.0,61925614.0,ena RUN TAB 21 09 2023 13:32:15:282 66618,0:126 1:126,A:3158918177;C:4616749707;G:4726409583;T:3100328707;N:2848554,126,126,,,3158918177,4616749707,4726409583,3100328707,2848554,ERX11454412,ERS16387654,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91674,0.91732,0.1668,0.16991,0.77569,0.77082,0.76006,0.76056,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14944,ERR12071786,ERX11454411,ERS16387653,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 3,SAMEA114398961,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66615,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-3_S68_L008_R1_001.fastq.gz 102-3_S68_L008_R2_001.fastq.gz,fastq fastq,20642076252.0,81913001.0,ena RUN TAB 21 09 2023 13:32:15:282 66616,0:126 1:126,A:3670199430;C:6557337612;G:6776033266;T:3629933781;N:8572163,126,126,,,3670199430,6557337612,6776033266,3629933781,8572163,ERX11454411,ERS16387653,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89984,0.89794,0.14393,0.14603,0.82079,0.82233,0.83939,0.8347,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14945,ERR12071785,ERX11454410,ERS16387652,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 2,SAMEA114398960,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66613,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 22,102-2_S31_L006_R1_001.fastq.gz 102-2_S31_L006_R2_001.fastq.gz,fastq fastq,23877621936.0,94752468.0,ena RUN TAB 21 09 2023 13:32:15:282 66614,0:126 1:126,A:3953279642;C:7876070920;G:8128759951;T:3914909602;N:4601821,126,126,,,3953279642,7876070920,8128759951,3914909602,4601821,ERX11454410,ERS16387652,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92602,0.85538,0.13016,0.12286,0.84508,0.84936,0.81613,0.88529,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14946,ERR12071784,ERX11454409,ERS16387651,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 1,SAMEA114398959,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66611,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-1_S64_L007_R1_001.fastq.gz 102-1_S64_L007_R2_001.fastq.gz,fastq fastq,20588587236.0,81700743.0,ena RUN TAB 21 09 2023 13:32:15:281 66612,0:126 1:126,A:3005365881;C:7167599347;G:7450281822;T:2959758690;N:5581496,126,126,,,3005365881,7167599347,7450281822,2959758690,5581496,ERX11454409,ERS16387651,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90225,0.89979,0.08909,0.09003,0.87071,0.87148,0.90162,0.83246,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14947,ERR12071783,ERX11454408,ERS16387650,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 4,SAMEA114398958,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66609,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-4_S30_L006_R1_001.fastq.gz 101-4_S30_L006_R2_001.fastq.gz,fastq fastq,12524862168.0,49701834.0,ena RUN TAB 21 09 2023 13:32:15:281 66610,0:126 1:126,A:2576891018;C:3688938717;G:3819872503;T:2436717421;N:2442509,126,126,,,2576891018,3688938717,3819872503,2436717421,2442509,ERX11454408,ERS16387650,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92878,0.92997,0.15322,0.15505,0.76315,0.76063,0.69783,0.67569,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14948,ERR12071782,ERX11454407,ERS16387649,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 3,SAMEA114398957,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66607,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-3_S34_L007_R1_001.fastq.gz 101-3_S34_L007_R2_001.fastq.gz,fastq fastq,11973395700.0,47513475.0,ena RUN TAB 21 09 2023 13:32:15:281 66608,0:126 1:126,A:2371769443;C:3605917515;G:3700919970;T:2292577579;N:2211193,126,126,,,2371769443,3605917515,3700919970,2292577579,2211193,ERX11454407,ERS16387649,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92217,0.92315,0.14568,0.1469,0.7724,0.76917,0.7521,0.75171,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14949,ERR12071781,ERX11454406,ERS16387648,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 2,SAMEA114398956,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66605,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-2_S63_L007_R2_001.fastq.gz 101-2_S63_L007_R1_001.fastq.gz,fastq fastq,10806069456.0,42881228.0,ena RUN TAB 21 09 2023 13:32:15:280 66606,0:126 1:126,A:2073944191;C:3321784426;G:3405352767;T:2002070301;N:2917771,126,126,,,2073944191,3321784426,3405352767,2002070301,2917771,ERX11454406,ERS16387648,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9236,0.92427,0.14372,0.14539,0.78084,0.78141,0.74294,0.78886,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14950,ERR12071780,ERX11454405,ERS16387647,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 1,SAMEA114398955,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66603,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-1_S67_L008_R1_001.fastq.gz 101-1_S67_L008_R2_001.fastq.gz,fastq fastq,17155416348.0,68077049.0,ena RUN TAB 21 09 2023 13:32:15:280 66604,0:126 1:126,A:3066178934;C:5490109793;G:5728819123;T:2863162486;N:7146012,126,126,,,3066178934,5490109793,5728819123,2863162486,7146012,ERX11454405,ERS16387647,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94204,0.94154,0.12549,0.1266,0.80653,0.80726,0.83737,0.8394,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14951,ERR12071779,ERX11454404,ERS16387646,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 4,SAMEA114398954,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66601,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-4_S33_L007_R1_001.fastq.gz 100-4_S33_L007_R2_001.fastq.gz,fastq fastq,20883396240.0,82870620.0,ena RUN TAB 21 09 2023 13:32:15:280 66602,0:126 1:126,A:4151890640;C:6231825391;G:6398810174;T:4097014520;N:3855515,126,126,,,4151890640,6231825391,6398810174,4097014520,3855515,ERX11454404,ERS16387646,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91603,0.9155,0.17576,0.1788,0.7781,0.7753,0.76044,0.78007,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14952,ERR12071778,ERX11454403,ERS16387645,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 3,SAMEA114398953,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66599,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-3_S29_L006_R1_001.fastq.gz 100-3_S29_L006_R2_001.fastq.gz,fastq fastq,17203126752.0,68266376.0,ena RUN TAB 21 09 2023 13:32:15:280 66600,0:126 1:126,A:3380289058;C:5172410817;G:5314572798;T:3332505592;N:3348487,126,126,,,3380289058,5172410817,5314572798,3332505592,3348487,ERX11454403,ERS16387645,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90457,0.90462,0.18582,0.18981,0.79947,0.79435,0.81648,0.81903,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14953,ERR12071777,ERX11454402,ERS16387644,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 2,SAMEA114398952,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:279 66597,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-2_S66_L008_R2_001.fastq.gz 100-2_S66_L008_R1_001.fastq.gz,fastq fastq,12181472100.0,48339175.0,ena RUN TAB 21 09 2023 13:32:15:279 66598,0:126 1:126,A:2169455038;C:3847243791;G:3951410714;T:2208297402;N:5065155,126,126,,,2169455038,3847243791,3951410714,2208297402,5065155,ERX11454402,ERS16387644,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.87873,0.8759,0.15894,0.16003,0.82446,0.82475,0.8538,0.82928,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14954,ERR12071776,ERX11454401,ERS16387643,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 1,SAMEA114398951,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:279 66595,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-1_S62_L007_R1_001.fastq.gz 100-1_S62_L007_R2_001.fastq.gz,fastq fastq,12598877592.0,49995546.0,ena RUN TAB 21 09 2023 13:32:15:279 66596,0:126 1:126,A:2443108338;C:3813175879;G:3922667630;T:2416483718;N:3442027,126,126,,,2443108338,3813175879,3922667630,2416483718,3442027,ERX11454401,ERS16387643,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9126,0.91287,0.18702,0.1895,0.79431,0.79373,0.78056,0.81778,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
21547,ERR1374860,ERX1445986,ERS1036031,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281340,SAMEA3728882,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728882|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:28Z|INSDC status:public|Submitter Id:63f8d9b0 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTTTCCAGCGT is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63f8d9b0 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#384,15545073,Illumina sequencing of library 15545073  constructed from sample accession ERS1036031 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTTTCCAGCGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#384.cram,cram,284058.0,2202.0,SC RUN 18668 1#384,0:54 1:75,A:76261;C:66156;G:64128;T:77513;N:0,54,75,,,76261,66156,64128,77513,0,ERX1445986,ERS1036031,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.0,0.0007,0.0,0.00069,1.0,1.0,,,54,75,T,T,mates < 9% mapping rate,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21548,ERR1374859,ERX1445985,ERS1036030,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281339,SAMEA3728881,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728881|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:28Z|INSDC status:public|Submitter Id:63ef8ae0 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTTGATTCTCG is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63ef8ae0 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#383,15545072,Illumina sequencing of library 15545072  constructed from sample accession ERS1036030 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTTGATTCTCG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#383.cram,cram,1304835.0,10115.0,SC RUN 18668 1#383,0:54 1:75,A:352669;C:266861;G:245412;T:439893;N:0,54,75,,,352669,266861,245412,439893,0,ERX1445985,ERS1036030,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.31511,0.42794,0.3151,0.42793,1.0,1.0,,,54,75,T,B,mate1 technical by mapping diff,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21549,ERR1374858,ERX1445984,ERS1036029,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281338,SAMEA3728880,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728880|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:27Z|INSDC status:public|Submitter Id:63e21d60 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTTCGTGTAGA is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63e21d60 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#382,15545071,Illumina sequencing of library 15545071  constructed from sample accession ERS1036029 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTTCGTGTAGA.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#382.cram,cram,1003104.0,7776.0,SC RUN 18668 1#382,0:54 1:75,A:275526;C:213901;G:190809;T:322868;N:0,54,75,,,275526,213901,190809,322868,0,ERX1445984,ERS1036029,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.17365,0.18304,0.17364,0.18074,1.0,0.99995,,0.0,54,75,B,B,biological fallback assumption,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21550,ERR1374857,ERX1445983,ERS1036028,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281337,SAMEA3728879,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728879|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:04Z|INSDC last update:2016 01 19T16:49:27Z|INSDC status:public|Submitter Id:63d8ce90 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTTATTAGACG is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63d8ce90 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#381,15545070,Illumina sequencing of library 15545070  constructed from sample accession ERS1036028 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTTATTAGACG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#381.cram,cram,553023.0,4287.0,SC RUN 18668 1#381,0:54 1:75,A:147453;C:125606;G:113473;T:166491;N:0,54,75,,,147453,125606,113473,166491,0,ERX1445983,ERS1036028,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.07054,0.14591,0.07053,0.1459,1.0,1.0,,,54,75,B,B,mate1-mate2 similar by mapping diff,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21551,ERR1374856,ERX1445982,ERS1036027,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281336,SAMEA3728878,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728878|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:27Z|INSDC status:public|Submitter Id:63cfa6d0 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTTAGCTCGGT is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63cfa6d0 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#380,15545069,Illumina sequencing of library 15545069  constructed from sample accession ERS1036027 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTTAGCTCGGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#380.cram,cram,1052640.0,8160.0,SC RUN 18668 1#380,0:54 1:75,A:272334;C:229709;G:203715;T:346882;N:0,54,75,,,272334,229709,203715,346882,0,ERX1445982,ERS1036027,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.31077,0.37992,0.31076,0.37991,1.0,1.0,,,54,75,B,B,biological fallback assumption,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21552,ERR1374855,ERX1445981,ERS1036026,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281335,SAMEA3728877,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728877|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:26Z|INSDC status:public|Submitter Id:63c630f0 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTGCGATGTCC is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63c630f0 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#379,15545068,Illumina sequencing of library 15545068  constructed from sample accession ERS1036026 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTGCGATGTCC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#379.cram,cram,1640622.0,12718.0,SC RUN 18668 1#379,0:54 1:75,A:431283;C:344682;G:321969;T:542688;N:0,54,75,,,431283,344682,321969,542688,0,ERX1445981,ERS1036026,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.29581,0.39023,0.2958,0.39022,1.0,1.0,,,54,75,B,B,biological fallback assumption,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21553,ERR1374854,ERX1445980,ERS1036025,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281334,SAMEA3728876,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728876|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:26Z|INSDC status:public|Submitter Id:63bd0930 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTGCACTTAAT is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63bd0930 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#378,15545067,Illumina sequencing of library 15545067  constructed from sample accession ERS1036025 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTGCACTTAAT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#378.cram,cram,906999.0,7031.0,SC RUN 18668 1#378,0:54 1:75,A:245673;C:184416;G:171222;T:305688;N:0,54,75,,,245673,184416,171222,305688,0,ERX1445980,ERS1036025,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.24186,0.3482,0.24185,0.34819,1.0,1.0,,,54,75,B,B,mate1-mate2 similar by mapping diff,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21554,ERR1374853,ERX1445979,ERS1036024,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281333,SAMEA3728875,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728875|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:25Z|INSDC status:public|Submitter Id:63b3ba60 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTCTGGGAGAG is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63b3ba60 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#377,15545066,Illumina sequencing of library 15545066  constructed from sample accession ERS1036024 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTCTGGGAGAG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#377.cram,cram,390612.0,3028.0,SC RUN 18668 1#377,0:54 1:75,A:98977;C:98046;G:87213;T:106376;N:0,54,75,,,98977,98046,87213,106376,0,ERX1445979,ERS1036024,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.00038,0.00048,0.00037,0.00047,1.0,1.0,,,54,75,T,T,mates < 9% mapping rate,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21555,ERR1374852,ERX1445978,ERS1036023,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281332,SAMEA3728874,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728874|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:04Z|INSDC last update:2016 01 19T16:49:25Z|INSDC status:public|Submitter Id:63aa4480 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTCTGATTTTG is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63aa4480 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#376,15545065,Illumina sequencing of library 15545065  constructed from sample accession ERS1036023 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTCTGATTTTG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#376.cram,cram,1790907.0,13883.0,SC RUN 18668 1#376,0:54 1:75,A:508300;C:371765;G:340642;T:570200;N:0,54,75,,,508300,371765,340642,570200,0,ERX1445978,ERS1036023,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.22446,0.28647,0.22445,0.28626,1.0,0.99997,,0.0,54,75,B,B,biological fallback assumption,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21556,ERR1374851,ERX1445977,ERS1036022,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281331,SAMEA3728873,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728873|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:24Z|INSDC status:public|Submitter Id:63a11cc0 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTCGTGCTTTC is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:63a11cc0 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#375,15545064,Illumina sequencing of library 15545064  constructed from sample accession ERS1036022 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTCGTGCTTTC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#375.cram,cram,1313349.0,10181.0,SC RUN 18668 1#375,0:54 1:75,A:354351;C:262296;G:244012;T:452690;N:0,54,75,,,354351,262296,244012,452690,0,ERX1445977,ERS1036022,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.37529,0.46316,0.37528,0.45581,1.0,0.99993,,0.01754,54,75,B,B,biological fallback assumption,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined
21557,ERR1374850,ERX1445976,ERS1036021,ERP011007,PRJEB9859,Transcriptome profiling of single zebrafish cells,Transcriptome_profiling_of_single_zebrafish_cells-sc-2991,Transcriptome Analysis,Paired end sequence data from the Illumina HiSeq was prepared from individual wild type zebrafish cells. Generated to study  the transcriptome of single cells.,,,,Single cell TC6281330,SAMEA3728872,Wellcome Sanger Institute,ArrayExpress Species:Danio rerio|ENA first public:2016 04 26|ENA last update:2016 01 19|External Id:SAMEA3728872|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 04 26T14:06:05Z|INSDC last update:2016 01 19T16:49:24Z|INSDC status:public|Submitter Id:6397cdf0 a4bf 11e5 bdd9 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from single cells. An 11 base indexing sequence TTCGGCCCAAT is bases 11 to 21 of read 1 followed by CG and polyT.|sample name:6397cdf0 a4bf 11e5 bdd9 68b59976a382,,,,,,,,,Illumina MiSeq paired end sequencing,SC EXP 18668 1#374,15545063,Illumina sequencing of library 15545063  constructed from sample accession ERS1036021 for study accession ERP011007.  This is part of an Illumina multiplexed sequencing run 18668 1.  This submission includes reads tagged with the sequence TTCGGCCCAAT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina MiSeq,,ERP011007,Illumina MiSeq paired end sequencing,ENA FIRST PUBLIC:2016 04 26|ENA LAST UPDATE:2018 11 16,18668_1#374.cram,cram,9495561.0,73609.0,SC RUN 18668 1#374,0:54 1:75,A:2538234;C:2126986;G:1889072;T:2941269;N:0,54,75,,,2538234,2126986,1889072,2941269,0,ERX1445976,ERS1036021,ERA610021,European Nucleotide Archive,Wellcome Sanger Institute,2,0.12462,0.15044,0.12459,0.15041,0.99997,0.99997,1.0,0.0,54,75,B,B,biological fallback assumption,illumina,miseq,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2016-01-19,Undetermined,Undetermined,Undetermined,Undetermined