rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
131,DRR189373,DRX179838,DRS200446,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Dissociated cells from telencephalon of wild type adult zebrafish 3,SAMD00182216,,sample name:Cell Tel 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182216,DRX179838,Cell Tel 3,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182216,,,,4142772200.0,20713861.0,DRR189373,0:100 1:100,A:1185533233;C:877739936;G:877025820;T:1202041821;N:431390,100,100,,,1185533233,877739936,877025820,1202041821,431390,DRX179838,DRS200446,DRA008855,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.9018,0.90073,0.24953,0.25062,0.67884,0.67957,0.49957,0.5402,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
132,DRR189372,DRX179837,DRS200445,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Dissociated cells from telencephalon of wild type adult zebrafish 2,SAMD00182215,,sample name:Cell Tel 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182215,DRX179837,Cell Tel 2,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182215,,,,5233184600.0,26165923.0,DRR189372,0:100 1:100,A:1522745973;C:1082449570;G:1076728796;T:1550682585;N:577676,100,100,,,1522745973,1082449570,1076728796,1550682585,577676,DRX179837,DRS200445,DRA008855,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89481,0.89419,0.27137,0.27018,0.67639,0.67671,0.54949,0.55323,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
133,DRR189371,DRX179836,DRS200444,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Dissociated cells from telencephalon of wild type adult zebrafish 1,SAMD00182214,,sample name:Cell Tel 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182214,DRX179836,Cell Tel 1,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182214,,,,4407578800.0,22037894.0,DRR189371,0:100 1:100,A:1270948410;C:923917605;G:922122984;T:1290126238;N:463563,100,100,,,1270948410,923917605,922122984,1290126238,463563,DRX179836,DRS200444,DRA008855,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.90057,0.90117,0.24092,0.24074,0.67598,0.67775,0.53184,0.53433,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
134,DRR189370,DRX179835,DRS200415,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 5,SAMD00182213,,sample name:h62A GFP Tel 5|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182213,DRX179835,h62A GFP Tel 5,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182213,,,,2904332400.0,14521662.0,DRR189370,0:100 1:100,A:821602205;C:620206517;G:623738781;T:838474359;N:310538,100,100,,,821602205,620206517,623738781,838474359,310538,DRX179835,DRS200415,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91925,0.91942,0.14636,0.14584,0.71127,0.71429,0.62818,0.40804,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
135,DRR189369,DRX179834,DRS200414,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 4,SAMD00182212,,sample name:h62A GFP Tel 4|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182212,DRX179834,h62A GFP Tel 4,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182212,,,,4670374000.0,23351870.0,DRR189369,0:100 1:100,A:1294304230;C:1030286884;G:1032728119;T:1312561064;N:493703,100,100,,,1294304230,1030286884,1032728119,1312561064,493703,DRX179834,DRS200414,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.92587,0.92725,0.12279,0.12312,0.71056,0.71293,0.57854,0.57882,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
136,DRR189368,DRX179833,DRS200413,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 3,SAMD00182211,,sample name:h62A GFP Tel 3|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182211,DRX179833,h62A GFP Tel 3,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182211,,,,11487645000.0,57438225.0,DRR189368,0:100 1:100,A:3127119396;C:2611288030;G:2547219656;T:3194997425;N:7020493,100,100,,,3127119396,2611288030,2547219656,3194997425,7020493,DRX179833,DRS200413,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91389,0.91371,0.11188,0.11336,0.74576,0.74639,0.56209,0.56027,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
137,DRR189367,DRX179832,DRS200412,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 2,SAMD00182210,,sample name:h62A GFP Tel 2|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182210,DRX179832,h62A GFP Tel 2,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182210,,,,13536257200.0,67681286.0,DRR189367,0:100 1:100,A:3723068804;C:3049411151;G:2951685691;T:3803778575;N:8312979,100,100,,,3723068804,3049411151,2951685691,3803778575,8312979,DRX179832,DRS200412,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.9136,0.91493,0.14102,0.14249,0.70534,0.70569,0.58385,0.58171,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
138,DRR189366,DRX179831,DRS200411,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 1,SAMD00182209,,sample name:h62A GFP Tel 1|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182209,DRX179831,h62A GFP Tel 1,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182209,,,,13057029600.0,65285148.0,DRR189366,0:100 1:100,A:3609997372;C:2912841795;G:2821782510;T:3704302917;N:8105006,100,100,,,3609997372,2912841795,2821782510,3704302917,8105006,DRX179831,DRS200411,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.90766,0.90786,0.13813,0.14007,0.73677,0.73777,0.55675,0.55743,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
139,DRR189365,DRX179830,DRS200432,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of SAGFF120A;UAS:GFP adult zebrafish 4,SAMD00182208,,sample name:120A GFP Tel 4|genotype:SAGFF120A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00182208,DRX179830,120A GFP Tel 4,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2000 paired end sequencing of SAMD00182208,,,,4105132200.0,20525661.0,DRR189365,0:100 1:100,A:1171500567;C:874901685;G:872257219;T:1186025546;N:447183,100,100,,,1171500567,874901685,872257219,1186025546,447183,DRX179830,DRS200432,DRA008853,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91106,0.90924,0.18729,0.18688,0.69406,0.69589,0.51697,0.51253,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
140,DRR189364,DRX179829,DRS200431,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of SAGFF120A;UAS:GFP adult zebrafish 3,SAMD00182207,,sample name:120A GFP Tel 3|genotype:SAGFF120A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182207,DRX179829,120A GFP Tel 3,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182207,,,,5062007400.0,25310037.0,DRR189364,0:100 1:100,A:1425414609;C:1105334563;G:1072448536;T:1458726402;N:83290,100,100,,,1425414609,1105334563,1072448536,1458726402,83290,DRX179829,DRS200431,DRA008853,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89598,0.89539,0.2645,0.2668,0.69591,0.6971,0.49901,0.49932,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
141,DRR189363,DRX179828,DRS200430,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of SAGFF120A;UAS:GFP adult zebrafish 2,SAMD00182206,,sample name:120A GFP Tel 2|genotype:SAGFF120A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182206,DRX179828,120A GFP Tel 2,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182206,,,,4148886200.0,20744431.0,DRR189363,0:100 1:100,A:1121946535;C:959659540;G:901840216;T:1165367793;N:72116,100,100,,,1121946535,959659540,901840216,1165367793,72116,DRX179828,DRS200430,DRA008853,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89808,0.89803,0.14106,0.14229,0.74057,0.74073,0.58127,0.58258,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
142,DRR189362,DRX179827,DRS200429,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of SAGFF120A;UAS:GFP adult zebrafish 1,SAMD00182205,,sample name:120A GFP Tel 1|genotype:SAGFF120A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182205,DRX179827,120A GFP Tel 1,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182205,,,,5621230800.0,28106154.0,DRR189362,0:100 1:100,A:1511319423;C:1299516107;G:1236814355;T:1573482053;N:98862,100,100,,,1511319423,1299516107,1236814355,1573482053,98862,DRX179827,DRS200429,DRA008853,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.90171,0.90205,0.11967,0.12059,0.74083,0.74113,0.61779,0.61682,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
143,DRR189361,DRX179826,DRS200425,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form whole brain of HuC:GFP adult zebrafish 4,SAMD00182204,,sample name:HuC GFP WB 4|genotype:HuC:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182204,DRX179826,HuC GFP WB 4,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182204,,,,3641515200.0,18207576.0,DRR189361,0:100 1:100,A:1038686472;C:777269450;G:777179582;T:1047994354;N:385342,100,100,,,1038686472,777269450,777179582,1047994354,385342,DRX179826,DRS200425,DRA008852,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91246,0.9127,0.18566,0.18547,0.70201,0.7024,0.47049,0.47102,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
144,DRR189360,DRX179825,DRS200424,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form whole brain of HuC:GFP adult zebrafish 3,SAMD00182203,,sample name:HuC GFP WB 3|genotype:HuC:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182203,DRX179825,HuC GFP WB 3,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182203,,,,4692211000.0,23461055.0,DRR189360,0:100 1:100,A:1310884816;C:1036187295;G:997846600;T:1347212024;N:80265,100,100,,,1310884816,1036187295,997846600,1347212024,80265,DRX179825,DRS200424,DRA008852,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89626,0.89739,0.18174,0.18338,0.72443,0.72588,0.4577,0.46684,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
145,DRR189359,DRX179824,DRS200423,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form whole brain of HuC:GFP adult zebrafish 2,SAMD00182202,,sample name:HuC GFP WB 2|genotype:HuC:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182202,DRX179824,HuC GFP WB 2,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182202,,,,7514071000.0,37570355.0,DRR189359,0:100 1:100,A:2098251885;C:1661407212;G:1597423444;T:2156861774;N:126685,100,100,,,2098251885,1661407212,1597423444,2156861774,126685,DRX179824,DRS200423,DRA008852,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89548,0.89691,0.26231,0.26473,0.70358,0.70471,0.4732,0.4753,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
146,DRR189358,DRX179823,DRS200422,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form whole brain of HuC:GFP adult zebrafish 1,SAMD00182201,,sample name:HuC GFP WB 1|genotype:HuC:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182201,DRX179823,HuC GFP WB 1,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182201,,,,5175824800.0,25879124.0,DRR189358,0:100 1:100,A:1424688686;C:1174545239;G:1128653980;T:1447848363;N:88532,100,100,,,1424688686,1174545239,1128653980,1447848363,88532,DRX179823,DRS200422,DRA008852,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91906,0.91958,0.20879,0.21088,0.74787,0.74876,0.45243,0.45221,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
147,DRR051067,DRX045959,DRS057267,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,These cells are from the same fish as h62A GFP plus Tel,GFP  cells from telencephalon of hspGFF62A;UAS:GFP transgenic zebrafish,SAMD00044994,,sample name:h62A GFP minus Tel|tissue type:brain|genotype:hspGFF62A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044994,DRX045959,h62A GFP minus Tel,1,cDNA synthesis : clontech SMARTer v3  > Library prep : Illumina Nextera XT DNA Library Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044994,,,,17913873600.0,89569368.0,DRR051067,0:100 1:100,A:5009629866;C:3957379257;G:3797879751;T:5144366288;N:4618438,100,100,,,5009629866,3957379257,3797879751,5144366288,4618438,DRX045959,DRS057267,DRA004277,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89075,0.89087,0.23044,0.23295,0.69493,0.69769,0.5366,0.54852,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2018-01-06,Undetermined,Undetermined,Brain,Nervous System
148,DRR051066,DRX045958,DRS057275,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Collected from 40 adult fish by using FACS,GFP+ cells from telencephalon of hspGFF62A;UAS:GFP transgenic zebrafish,SAMD00044995,,sample name:h62A GFP plus Tel|tissue type:brain|genotype:hspGFF62A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044995,DRX045958,h62A GFP plus Tel,1,cDNA synthesis : clontech SMARTer v3  > Library prep : Illumina Nextera XT DNA Library Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044995,,,,17421256000.0,87106280.0,DRR051066,0:100 1:100,A:4859368314;C:3846634012;G:3717526030;T:4993260256;N:4467388,100,100,,,4859368314,3846634012,3717526030,4993260256,4467388,DRX045958,DRS057275,DRA004276,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.88127,0.88114,0.22941,0.23155,0.72161,0.72437,0.49284,0.49449,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
149,DRR051065,DRX045957,DRS057272,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Telencephalon from adult zebrafrish  30 min post light and electrical shock association in non trace Two Way Active Avoidance conditioning,CS+US telencephalon 30 min post TWAA,SAMD00044990,,sample name:CS+US Tel 30|tissue type:brain|genotype:WT,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044990,DRX045957,CS+US Tel 30,1,Illumina Truseq RNA Library Prep Kit v2,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044990,,,,20239329200.0,101196646.0,DRR051065,0:100 1:100,A:5347169722;C:4777492672;G:4750401220;T:5361888239;N:2377347,100,100,,,5347169722,4777492672,4750401220,5361888239,2377347,DRX045957,DRS057272,DRA004275,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.93576,0.93353,0.13979,0.14019,0.70043,0.70203,0.48989,0.49214,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
150,DRR051064,DRX045956,DRS057265,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Telencephalon from adult zebrafrish  30 min post light stimulation in Two Way Active Avoidance coditioning,CS telencephalon 30 min post TWAA,SAMD00044991,,sample name:CS Tel 30|tissue type:brain|genotype:WT,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044991,DRX045956,CS Tel 30,1,Illumina Truseq RNA Library Prep Kit v2,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044991,,,,29268319600.0,146341598.0,DRR051064,0:100 1:100,A:7777792429;C:6878969107;G:6819275179;T:7788795270;N:3487615,100,100,,,7777792429,6878969107,6819275179,7788795270,3487615,DRX045956,DRS057265,DRA004274,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.93646,0.93664,0.14051,0.14191,0.70199,0.70374,0.49256,0.49684,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
151,DRR051063,DRX045955,DRS057266,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,These cells are from the same fish as 120A GFP plus Tel,GFP  cells from telencephalon of SAGFFLF120A;UAS:GFP transgenic zebrafish,SAMD00044987,,sample name:120A GFP minus Tel|tissue type:brain|genotype:SAGFFLF120A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044987,DRX045955,120A GFP minus Tel,1,cDNA synthesis : clontech SMARTer v2  > Library prep : Illumina TrunSeq DNA Sample Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044987,,,,15411400120.0,76294060.0,DRR051063,0:101 1:101,A:4583085286;C:3082552426;G:3134669086;T:4606055524;N:5037798,101,101,,,4583085286,3082552426,3134669086,4606055524,5037798,DRX045955,DRS057266,DRA004273,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89184,0.8842,0.30246,0.30334,0.69232,0.705,0.52824,0.51492,101,101,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,smarter,bulk,unknown,unknown,,Japan,2018-01-06,Undetermined,Undetermined,Brain,Nervous System
152,DRR051062,DRX045954,DRS057271,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Collected from 40 adult fish by using FACS,GFP+ cells from telencephalon of SAGFFLF120A;UAS:GFP transgenic zebrafish,SAMD00044988,,sample name:120A GFP plus Tel|tissue type:brain|genotype:SAGFFLF120A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044988,DRX045954,120A GFP plus Tel,1,cDNA synthesis : clontech SMARTer v2  > Library prep : Illumina TrunSeq DNA Sample Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044988,,,,9916242014.0,49090307.0,DRR051062,0:101 1:101,A:2991626000;C:1935418605;G:1974202781;T:3011783158;N:3211470,101,101,,,2991626000,1935418605,1974202781,3011783158,3211470,DRX045954,DRS057271,DRA004272,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.87779,0.86382,0.2948,0.28993,0.72809,0.74075,0.50317,0.49169,101,101,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,smarter,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
153,DRR051061,DRX045953,DRS057274,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Collected from 10 adult fish by using FACS,GFP+ cells from whole brain of SAGFFLF231A;UAS:GFP transgenic zebrafish,SAMD00044989,,sample name:231A GFP plus WB|tissue type:brain|genotype:SAGFFLF231A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044989,DRX045953,231A GFP plus WB,1,cDNA synthesis : clontech SMARTer v2  > Library prep : Illumina TrunSeq DNA Sample Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044989,,,,27230654000.0,136153270.0,DRR051061,0:101 1:99,A:7929214734;C:5506861409;G:5563593280;T:8059057191;N:171927386,101,99,,,7929214734,5506861409,5563593280,8059057191,171927386,DRX045953,DRS057274,DRA004271,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.88462,0.88282,0.26215,0.26067,0.72468,0.73555,0.48369,0.48285,101,99,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,smarter,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
154,DRR051060,DRX045952,DRS057269,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Telencephalon of adult zebrafrish barin,zebrafihsh telencephalon,SAMD00044992,,sample name:Tel|tissue type:brain|genotype:WT,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044992,DRX045952,Tel,1,Illumina Truseq RNA Library Prep Kit v2,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044992,,,,20006128076.0,99040238.0,DRR051060,0:101 1:101,A:5393556813;C:4623783008;G:4592812230;T:5384415659;N:11560366,101,101,,,5393556813,4623783008,4592812230,5384415659,11560366,DRX045952,DRS057269,DRA004270,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.94215,0.94001,0.16398,0.16585,0.69331,0.69479,0.4872,0.48516,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
155,DRR051059,DRX045951,DRS057270,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Whole brain of adult zebrafish,zebrafish whole brain,SAMD00044993,,sample name:WB|tissue type:brain|genotype:WT,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044993,DRX045951,WB,1,Illumina Truseq RNA Library Prep Kit v2,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044993,,,,25283347776.0,125165088.0,DRR051059,0:101 1:101,A:6845696813;C:5810492697;G:5775241119;T:6837142940;N:14774207,101,101,,,6845696813,5810492697,5775241119,6837142940,14774207,DRX045951,DRS057270,DRA004269,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.94203,0.94067,0.15107,0.15158,0.68651,0.6882,0.49596,0.49589,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
156,DRR067143,DRX061087,DRS034141,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Bergmann glial cells using Tg line SAGFFLF251A sample 2,SAMD00057666,,sample name:Zebrafish 251A 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:bergmann glial cells|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057666,DRX061087,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057666,,,,2040373922.0,10100861.0,DRR067143,0:101 1:101,A:577691972;C:445051245;G:472607289;T:544962357;N:61059,101,101,,,577691972,445051245,472607289,544962357,61059,DRX061087,DRS034141,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.83407,0.83802,0.12612,0.12666,0.73675,0.74079,0.4948,0.49282,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
157,DRR067142,DRX061086,DRS034140,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Bergmann glial cells using Tg line SAGFFLF251A sample 1,SAMD00057665,,sample name:Zebrafish 251A 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:bergmann glial cells|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057665,DRX061086,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057665,,,,2033951534.0,10069067.0,DRR067142,0:101 1:101,A:589558269;C:431084184;G:460501802;T:552746498;N:60781,101,101,,,589558269,431084184,460501802,552746498,60781,DRX061086,DRS034140,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.8958,0.90339,0.13764,0.1385,0.72563,0.72865,0.49373,0.49916,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
158,DRR067141,DRX061085,DRS034139,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Purkinje cells using Tg line aldoca:GAP Venus sample 3,SAMD00057664,,sample name:Zebrafish aldoca 03|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:purkinje cells|dev stage:14 dpf|replicate:biological replicate 3,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057664,DRX061085,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057664,,,,1931908608.0,9563904.0,DRR067141,0:101 1:101,A:558444394;C:409856521;G:429168864;T:534380258;N:58571,101,101,,,558444394,409856521,429168864,534380258,58571,DRX061085,DRS034139,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.88632,0.89109,0.15695,0.15773,0.75694,0.7599,0.46875,0.49236,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
159,DRR067140,DRX061084,DRS034138,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Purkinje cells using Tg line aldoca:GAP Venus sample 2,SAMD00057663,,sample name:Zebrafish aldoca 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:purkinje cells|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057663,DRX061084,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057663,,,,1851108406.0,9163903.0,DRR067140,0:101 1:101,A:537339104;C:390802782;G:416335529;T:506575762;N:55229,101,101,,,537339104,390802782,416335529,506575762,55229,DRX061084,DRS034138,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.9055,0.91341,0.1515,0.15333,0.76495,0.76719,0.49219,0.49012,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
160,DRR067139,DRX061083,DRS034137,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Purkinje cells using Tg line aldoca:GAP Venus sample 1,SAMD00057662,,sample name:Zebrafish aldoca 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:purkinje cells|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057662,DRX061083,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057662,,,,2000078962.0,9901381.0,DRR067139,0:101 1:101,A:583169141;C:420603631;G:446949308;T:549297272;N:59610,101,101,,,583169141,420603631,446949308,549297272,59610,DRX061083,DRS034137,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.9056,0.90932,0.15092,0.15179,0.76173,0.7654,0.49028,0.49604,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
161,DRR067138,DRX061082,DRS034136,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for granule cells using Tg line gSA2AzGFF152B sample 2,SAMD00057661,,sample name:Zebrafish 152B 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:granule cells|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057661,DRX061082,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057661,,,,1997432156.0,9888278.0,DRR067138,0:101 1:101,A:578466824;C:423038930;G:449779231;T:546086391;N:60780,101,101,,,578466824,423038930,449779231,546086391,60780,DRX061082,DRS034136,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.90322,0.91109,0.1463,0.14811,0.75452,0.75633,0.48303,0.48557,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
162,DRR067137,DRX061081,DRS034135,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for granule cells using Tg line gSA2AzGFF152B sample 1,SAMD00057660,,sample name:Zebrafish 152B 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:granule cells|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057660,DRX061081,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057660,,,,1908437218.0,9447709.0,DRR067137,0:101 1:101,A:552150223;C:401993096;G:427236477;T:527000978;N:56444,101,101,,,552150223,401993096,427236477,527000978,56444,DRX061081,DRS034135,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.90474,0.91043,0.17336,0.17401,0.76108,0.76359,0.483,0.48537,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
163,DRR067136,DRX061080,DRS034134,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Inferior olive nuclei using Tg line hspGFFDMC28C sample 3,SAMD00057659,,sample name:Zebrafish 28C 03|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:hindbrain|cell type:inferior olive nuclei|dev stage:14 dpf|replicate:biological replicate 3,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057659,DRX061080,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057659,,,,1982971178.0,9816689.0,DRR067136,0:101 1:101,A:504064758;C:488246993;G:520130333;T:470469799;N:59295,101,101,,,504064758,488246993,520130333,470469799,59295,DRX061080,DRS034134,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.53386,0.54375,0.06072,0.06189,0.76351,0.76641,0.4872,0.48857,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
164,DRR067135,DRX061079,DRS034133,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Inferior olive nuclei using Tg line hspGFFDMC28C sample 2,SAMD00057658,,sample name:Zebrafish 28C 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:hindbrain|cell type:inferior olive nuclei|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057658,DRX061079,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057658,,,,1961048320.0,9708160.0,DRR067135,0:101 1:101,A:557469151;C:424691789;G:455244994;T:523583601;N:58785,101,101,,,557469151,424691789,455244994,523583601,58785,DRX061079,DRS034133,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.84854,0.8526,0.10311,0.1042,0.75227,0.75499,0.49321,0.49435,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
165,DRR067134,DRX061078,DRS034132,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Inferior olive nuclei using Tg line hspGFFDMC28C sample 1,SAMD00057657,,sample name:Zebrafish 28C 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:hindbrain|cell type:inferior olive nuclei|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057657,DRX061078,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057657,,,,1931802760.0,9563380.0,DRR067134,0:101 1:101,A:546501397;C:422500710;G:452200521;T:510543489;N:56643,101,101,,,546501397,422500710,452200521,510543489,56643,DRX061078,DRS034132,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.85439,0.85613,0.09867,0.09897,0.76871,0.77082,0.48943,0.48936,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
166,DRR067133,DRX061077,DRS034131,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for eurydendroid cells using Tg line hspzGFFgDMC156A sample 2,SAMD00057656,,sample name:Zebrafish 156A 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:eurydendroid cells|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057656,DRX061077,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057656,,,,2054289096.0,10169748.0,DRR067133,0:101 1:101,A:563764176;C:464768863;G:497506420;T:528187503;N:62134,101,101,,,563764176,464768863,497506420,528187503,62134,DRX061077,DRS034131,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.69311,0.6968,0.12309,0.12389,0.76428,0.76676,0.48379,0.48227,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
167,DRR067132,DRX061076,DRS034130,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells  Purkinje cells  IO neurons  and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for eurydendroid cells using Tg line hspzGFFgDMC156A sample 1,SAMD00057655,,sample name:Zebrafish 156A 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center  Nagoya University|tissue type:cerebellum|cell type:eurydendroid cells|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057655,DRX061076,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>202</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>102</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057655,,,,2152665722.0,10656761.0,DRR067132,0:101 1:101,A:585252781;C:495382257;G:522677749;T:549287359;N:65576,101,101,,,585252781,495382257,522677749,549287359,65576,DRX061076,DRS034130,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.67771,0.68253,0.12949,0.1303,0.77216,0.77542,0.4951,0.49452,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System
260,DRR162481,DRX153100,DRS083161,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 39 mpf zebrafish replicate5,SAMD00152429,,sample name:b39 5|age:39 month|biological replicate:5|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152429,DRX153100,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152429,,,,1252728200.0,6263641.0,DRR162481,0:100 1:100,A:367502128;C:257734618;G:257260262;T:368202459;N:2028733,100,100,,,367502128,257734618,257260262,368202459,2028733,DRX153100,DRS083161,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92142,0.8899,0.17636,0.1685,0.70686,0.7166,0.53271,0.54366,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
261,DRR162480,DRX153099,DRS083160,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 39 mpf zebrafish replicate4,SAMD00152428,,sample name:b39 4|age:39 month|biological replicate:4|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152428,DRX153099,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152428,,,,1426772800.0,7133864.0,DRR162480,0:100 1:100,A:408404333;C:303657580;G:303201065;T:408996815;N:2513007,100,100,,,408404333,303657580,303201065,408996815,2513007,DRX153099,DRS083160,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92435,0.89379,0.16127,0.15446,0.69844,0.70554,0.53646,0.533,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
262,DRR162479,DRX153098,DRS083159,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 39 mpf zebrafish replicate3,SAMD00152427,,sample name:b39 3|age:39 month|biological replicate:3|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152427,DRX153098,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152427,,,,1197809800.0,5989049.0,DRR162479,0:100 1:100,A:348955835;C:249141949;G:248747996;T:348970452;N:1993568,100,100,,,348955835,249141949,248747996,348970452,1993568,DRX153098,DRS083159,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92402,0.89389,0.1731,0.1668,0.70938,0.72892,0.55061,0.56711,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
263,DRR162478,DRX153097,DRS083158,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 39 mpf zebrafish replicate2,SAMD00152426,,sample name:b39 2|age:39 month|biological replicate:2|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152426,DRX153097,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152426,,,,1389632200.0,6948161.0,DRR162478,0:100 1:100,A:397337261;C:295934915;G:295794427;T:398240347;N:2325250,100,100,,,397337261,295934915,295794427,398240347,2325250,DRX153097,DRS083158,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92803,0.89621,0.15624,0.14998,0.69988,0.70985,0.54263,0.53655,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
264,DRR162477,DRX153096,DRS083157,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 39 mpf zebrafish replicate1,SAMD00152425,,sample name:b39 1|age:39 month|biological replicate:1|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152425,DRX153096,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152425,,,,1413538400.0,7067692.0,DRR162477,0:100 1:100,A:427890787;C:277522619;G:278488081;T:427755965;N:1880948,100,100,,,427890787,277522619,278488081,427755965,1880948,DRX153096,DRS083157,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90967,0.88004,0.20536,0.19647,0.71384,0.72021,0.55413,0.55769,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
265,DRR162476,DRX153095,DRS083156,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 16 mpf zebrafish replicate5,SAMD00152424,,sample name:b16 5|age:16 month|biological replicate:5|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152424,DRX153095,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152424,,,,1256635000.0,6283175.0,DRR162476,0:100 1:100,A:385448452;C:241878013;G:242164401;T:385408510;N:1735624,100,100,,,385448452,241878013,242164401,385408510,1735624,DRX153095,DRS083156,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91032,0.87693,0.19953,0.19064,0.72575,0.73474,0.49199,0.5657,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
266,DRR162475,DRX153094,DRS083155,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 16 mpf zebrafish replicate4,SAMD00152423,,sample name:b16 4|age:16 month|biological replicate:4|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152423,DRX153094,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152423,,,,2577820600.0,12889103.0,DRR162475,0:100 1:100,A:783031561;C:503484597;G:503899732;T:784003178;N:3401532,100,100,,,783031561,503484597,503899732,784003178,3401532,DRX153094,DRS083155,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91098,0.87948,0.20945,0.19993,0.71758,0.72543,0.56796,0.56453,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
267,DRR162474,DRX153093,DRS083154,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 16 mpf zebrafish replicate3,SAMD00152422,,sample name:b16 3|age:16 month|biological replicate:3|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152422,DRX153093,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152422,,,,1147257600.0,5736288.0,DRR162474,0:100 1:100,A:357829214;C:214920802;G:215124134;T:357870811;N:1512639,100,100,,,357829214,214920802,215124134,357870811,1512639,DRX153093,DRS083154,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90822,0.88184,0.20468,0.19612,0.73992,0.74627,0.58395,0.50711,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
268,DRR162473,DRX153092,DRS083153,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 16 mpf zebrafish replicate2,SAMD00152421,,sample name:b16 2|age:16 month|biological replicate:2|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152421,DRX153092,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152421,,,,1399740200.0,6998701.0,DRR162473,0:100 1:100,A:420492615;C:278464854;G:278362101;T:420657546;N:1763084,100,100,,,420492615,278464854,278362101,420657546,1763084,DRX153092,DRS083153,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91795,0.88458,0.19015,0.18122,0.71374,0.72318,0.55157,0.55554,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
269,DRR162472,DRX153091,DRS083152,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 16 mpf zebrafish replicate1,SAMD00152420,,sample name:b16 1|age:16 month|biological replicate:1|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152420,DRX153091,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152420,,,,1109512400.0,5547562.0,DRR162472,0:100 1:100,A:336800225;C:217086705;G:217195789;T:336929233;N:1500448,100,100,,,336800225,217086705,217195789,336929233,1500448,DRX153091,DRS083152,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91025,0.87734,0.20046,0.19197,0.71774,0.72482,0.52937,0.56111,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
270,DRR162471,DRX153090,DRS083151,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 7 mpf zebrafish replicate5,SAMD00152419,,sample name:b07 5|age:7 month|biological replicate:5|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152419,DRX153090,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152419,,,,1472475400.0,7362377.0,DRR162471,0:100 1:100,A:448100356;C:287211180;G:287652290;T:447581010;N:1930564,100,100,,,448100356,287211180,287652290,447581010,1930564,DRX153090,DRS083151,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90584,0.87079,0.21738,0.2083,0.71415,0.7344,0.5518,0.55812,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
271,DRR162470,DRX153089,DRS083150,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 7 mpf zebrafish replicate4,SAMD00152418,,sample name:b07 4|age:7 month|biological replicate:4|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152418,DRX153089,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152418,,,,1173234000.0,5866170.0,DRR162470,0:100 1:100,A:349147253;C:236479543;G:237153294;T:348938811;N:1515099,100,100,,,349147253,236479543,237153294,348938811,1515099,DRX153089,DRS083150,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91933,0.88973,0.18681,0.17878,0.71108,0.71867,0.5448,0.55274,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
272,DRR162469,DRX153088,DRS083149,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 7 mpf zebrafish replicate3,SAMD00152417,,sample name:b07 3|age:7 month|biological replicate:3|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152417,DRX153088,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152417,,,,1322055600.0,6610278.0,DRR162469,0:100 1:100,A:387777992;C:270907178;G:273067585;T:387904272;N:2398573,100,100,,,387777992,270907178,273067585,387904272,2398573,DRX153088,DRS083149,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91753,0.88469,0.17775,0.17007,0.70656,0.71685,0.5466,0.54528,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
273,DRR162468,DRX153087,DRS083148,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 7 mpf zebrafish replicate2,SAMD00152416,,sample name:b07 2|age:7 month|biological replicate:2|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152416,DRX153087,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152416,,,,1160349000.0,5801745.0,DRR162468,0:100 1:100,A:350211581;C:228387129;G:229679563;T:349919152;N:2151575,100,100,,,350211581,228387129,229679563,349919152,2151575,DRX153087,DRS083148,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91146,0.87717,0.20422,0.19494,0.7083,0.72082,0.55642,0.56386,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
274,DRR162467,DRX153086,DRS083147,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 7 mpf zebrafish replicate1,SAMD00152415,,sample name:b07 1|age:7 month|biological replicate:1|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152415,DRX153086,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152415,,,,1162314600.0,5811573.0,DRR162467,0:100 1:100,A:344932586;C:233867698;G:235450342;T:345967852;N:2096122,100,100,,,344932586,233867698,235450342,345967852,2096122,DRX153086,DRS083147,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91102,0.88248,0.1897,0.18307,0.70786,0.71687,0.55008,0.54646,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
275,DRR162466,DRX153085,DRS083146,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 2 mpf zebrafish replicate5,SAMD00152414,,sample name:b02 5|age:2 month|biological replicate:5|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152414,DRX153085,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152414,,,,1184455800.0,5922279.0,DRR162466,0:100 1:100,A:357035323;C:233685447;G:235595745;T:356085837;N:2053448,100,100,,,357035323,233685447,235595745,356085837,2053448,DRX153085,DRS083146,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90819,0.87146,0.20659,0.19751,0.71246,0.72251,0.52523,0.52531,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
276,DRR162465,DRX153084,DRS083145,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 2 mpf zebrafish replicate4,SAMD00152413,,sample name:b02 4|age:2 month|biological replicate:4|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152413,DRX153084,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152413,,,,1389141000.0,6945705.0,DRR162465,0:100 1:100,A:423029010;C:270113394;G:270981952;T:422602360;N:2414284,100,100,,,423029010,270113394,270981952,422602360,2414284,DRX153084,DRS083145,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90961,0.87739,0.21147,0.20152,0.71492,0.72563,0.53639,0.53779,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
277,DRR162464,DRX153083,DRS083144,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 2 mpf zebrafish replicate3,SAMD00152412,,sample name:b02 3|age:2 month|biological replicate:3|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152412,DRX153083,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152412,,,,2322459000.0,11612295.0,DRR162464,0:100 1:100,A:701085106;C:457451297;G:460424165;T:699185460;N:4312972,100,100,,,701085106,457451297,460424165,699185460,4312972,DRX153083,DRS083144,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92479,0.89382,0.16108,0.1547,0.73312,0.74121,0.55089,0.55507,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
278,DRR162463,DRX153082,DRS083143,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 2 mpf zebrafish replicate2,SAMD00152411,,sample name:b02 2|age:2 month|biological replicate:2|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152411,DRX153082,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152411,,,,1188406400.0,5942032.0,DRR162463,0:100 1:100,A:354575190;C:238508166;G:239587004;T:353623510;N:2112530,100,100,,,354575190,238508166,239587004,353623510,2112530,DRX153082,DRS083143,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90922,0.8787,0.20709,0.2009,0.71614,0.7362,0.52768,0.53049,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
279,DRR162462,DRX153081,DRS083142,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain  gill  heart  liver and muscle from 2 month   7 month   16 month  and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al.  2014.,,,,brain sample from 2 mpf zebrafish replicate1,SAMD00152410,,sample name:b02 1|age:2 month|biological replicate:1|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152410,DRX153081,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152410,,,,1033492200.0,5167461.0,DRR162462,0:100 1:100,A:310135217;C:205335529;G:207428012;T:308760225;N:1833217,100,100,,,310135217,205335529,207428012,308760225,1833217,DRX153081,DRS083142,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.9176,0.8829,0.18651,0.17848,0.72099,0.73119,0.54307,0.54697,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
8064,ERR035545,ERX013540,ERS017861,ERP000447,PRJEB2368,Sanger zebrafish sequencing,E-MTAB-460,Other,,,,Protocols: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Zebrafish adult brain,SAMEA782568,Wellcome Sanger Institute,ENA first public:2011 02 03|ENA last update:2018 03 08|External Id:SAMEA782568|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2011 02 03T12:40:41Z|INSDC last update:2018 03 08T15:25:22Z|INSDC status:public|StrainOrLine:Singapore|Submitter Id:E MTAB 460:Zebrafish adult brain|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|organism part:brain|sample name:E MTAB 460:Zebrafish adult brain|sex:mixed,,,,,,,,,Sanger zebrafish sequencing,E MTAB 460 part2:5625 1,ZFbrain 2 RNA 1523492,Sanger zebrafish sequencing,Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was enriched for polyA+ RNA by 2 rounds of polyA pull down with magnetic beads and included a DNase treatment between the 2 rounds. RNA was chemically fragmented  LiCl precipitated  reverse transcribed with random primers  a second strand synthesized and made into a standard Illumina library with a fragment size of 250 to 300 bp.,Experimental Factor: ORGANISM PART:brain,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina Genome Analyzer II,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>160</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Technical Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>77</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>2</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>85</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP000447,Illumina Genome Analyzer II paired end sequencing; Sanger zebrafish sequencing,ENA FIRST PUBLIC:2011 06 14|ENA LAST UPDATE:2018 11 16,5625_1.srf,srf,4576566720.0,28603542.0,E MTAB 460 part2:5625 1.srf,0:76 1:8 2:76,A:1227184716;C:942715631;G:951021478;T:1219839320;N:6977239,76,8,76,,1227184716,942715631,951021478,1219839320,6977239,ERX013540,ERS017861,ERA033503,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.86503,0.86182,0.22392,0.22256,0.69664,0.69865,0.51454,0.51606,76,76,B,B,biological fallback assumption,illumina,early_illumina,unknown,poly_a,unknown,bulk,unknown,unknown,,United Kingdom,2011-02-03,Adult,Adult,Brain,Nervous System
8069,ERR023147,ERX009449,ERS017861,ERP000447,PRJEB2368,Sanger zebrafish sequencing,E-MTAB-460,Other,,,,Protocols: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Zebrafish adult brain,SAMEA782568,Wellcome Sanger Institute,ENA first public:2011 02 03|ENA last update:2018 03 08|External Id:SAMEA782568|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2011 02 03T12:40:41Z|INSDC last update:2018 03 08T15:25:22Z|INSDC status:public|StrainOrLine:Singapore|Submitter Id:E MTAB 460:Zebrafish adult brain|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|organism part:brain|sample name:E MTAB 460:Zebrafish adult brain|sex:mixed,,,,,,,,,Sanger zebrafish sequencing,E MTAB 460:3537 7,RNA from Zebrafish adult brain,Sanger zebrafish sequencing,Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Experimental Factor: ORGANISM PART:brain,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina Genome Analyzer II,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>152</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>77</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP000447,Illumina Genome Analyzer II paired end sequencing; Sanger zebrafish sequencing,ENA FIRST PUBLIC:2011 02 03|ENA LAST UPDATE:2018 11 16,3537_7.srf,srf,3047469128.0,20049139.0,E MTAB 460:3537 7.srf,0:76 1:76,A:951431181;C:561974807;G:565451314;T:964940357;N:3671469,76,76,,,951431181,561974807,565451314,964940357,3671469,ERX009449,ERS017861,ERA015648,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.89672,0.89537,0.31092,0.31152,0.75724,0.7583,0.56639,0.56663,76,76,B,B,biological fallback assumption,illumina,early_illumina,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2011-02-03,Adult,Adult,Brain,Nervous System
8070,ERR023144,ERX009448,ERS017861,ERP000447,PRJEB2368,Sanger zebrafish sequencing,E-MTAB-460,Other,,,,Protocols: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Zebrafish adult brain,SAMEA782568,Wellcome Sanger Institute,ENA first public:2011 02 03|ENA last update:2018 03 08|External Id:SAMEA782568|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2011 02 03T12:40:41Z|INSDC last update:2018 03 08T15:25:22Z|INSDC status:public|StrainOrLine:Singapore|Submitter Id:E MTAB 460:Zebrafish adult brain|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|organism part:brain|sample name:E MTAB 460:Zebrafish adult brain|sex:mixed,,,,,,,,,Sanger zebrafish sequencing,E MTAB 460:3212 6,RNA from Zebrafish adult brain,Sanger zebrafish sequencing,Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Experimental Factor: ORGANISM PART:brain,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina Genome Analyzer II,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>152</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>77</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP000447,Illumina Genome Analyzer II paired end sequencing; Sanger zebrafish sequencing,ENA FIRST PUBLIC:2011 02 03|ENA LAST UPDATE:2018 11 16,3212_6.srf,srf,1970995248.0,12967074.0,E MTAB 460:3212 6.srf,0:76 1:76,A:601407561;C:370471604;G:371296333;T:608476094;N:19343656,76,76,,,601407561,370471604,371296333,608476094,19343656,ERX009448,ERS017861,ERA015648,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.90042,0.89919,0.30246,0.30122,0.75534,0.75349,0.57375,0.56725,76,76,B,B,biological fallback assumption,illumina,early_illumina,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2011-02-03,Adult,Adult,Brain,Nervous System
10170,ERR375749,ERX348126,ERS337052,ERP003756,PRJEB4464,Zebrafish olfactory transcriptomics,Zebrafish_olfactory_transcriptomics-sc-2013-08-12T10:32:48Z-2742,Transcriptome Analysis,The olfactory gene repertoire is largely species specific  shaped by the nature and necessity of chemosensory information for survival in each species' niche. The relative expression of olfactory receptors can be quantified by RNA sequencing. We investigated the olfactory transcriptome of zebrafish because : i of its phylogenetic location in the vertebrate tree  ii it has only one olfactory organ yet it has representatives of all the mammalian chemosensory receptor families  iii its biology is well known  iv is a commonly used model organism  and it is suitable for subsequent functional analysis.,,,,,SAMEA2168448,SC,ArrayExpress Genotype:Wildtype|ArrayExpress OrganismPart:Olfactory epithelium|ArrayExpress Sex:male|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 11 25T10:18:08Z|ENA LAST UPDATE:2018 03 08T16:38:06Z|External Id:SAMEA2168448|INSDC center name:SC|INSDC first public:2013 11 25T10:18:08Z|INSDC last update:2018 03 08T16:38:06Z|INSDC status:public|Submitter Id:ZF OE3 sc 2013 08 12T10:36:21Z 1677721|common name:zebrafish|sample description:RNA from OE|sample name:ZF OE3 sc 2013 08 12T10:36:21Z 1677721|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 10586 2#3,7941068,Illumina sequencing of library 7941068  constructed from sample accession ERS337052 for study accession ERP003756.  This is part of an Illumina multiplexed sequencing run 10586 2.  This submission includes reads tagged with the sequence TTAGGC.,Illumina cDNA protocol,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP003756,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2013 11 25|ENA LAST UPDATE:2018 11 16,10586_2#3.cram,cram,11298599000.0,56492995.0,SC RUN 10586 2#3,0:100 1:100,A:3356877540;C:2323057831;G:2299363865;T:3303865798;N:15433966,100,100,,,3356877540,2323057831,2299363865,3303865798,15433966,ERX348126,ERS337052,ERA267455,SC,Wellcome Sanger Institute,2,0.91061,0.90958,0.1659,0.16567,0.68398,0.68645,0.53954,0.54231,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2013-11-25,Undetermined,Undetermined,Brain,Nervous System
10171,ERR375748,ERX348125,ERS337051,ERP003756,PRJEB4464,Zebrafish olfactory transcriptomics,Zebrafish_olfactory_transcriptomics-sc-2013-08-12T10:32:48Z-2742,Transcriptome Analysis,The olfactory gene repertoire is largely species specific  shaped by the nature and necessity of chemosensory information for survival in each species' niche. The relative expression of olfactory receptors can be quantified by RNA sequencing. We investigated the olfactory transcriptome of zebrafish because : i of its phylogenetic location in the vertebrate tree  ii it has only one olfactory organ yet it has representatives of all the mammalian chemosensory receptor families  iii its biology is well known  iv is a commonly used model organism  and it is suitable for subsequent functional analysis.,,,,,SAMEA2168447,SC,ArrayExpress Genotype:Wildtype|ArrayExpress OrganismPart:Olfactory epithelium|ArrayExpress Sex:male|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 11 25T10:18:08Z|ENA LAST UPDATE:2018 03 08T16:38:13Z|External Id:SAMEA2168447|INSDC center name:SC|INSDC first public:2013 11 25T10:18:08Z|INSDC last update:2018 03 08T16:38:13Z|INSDC status:public|Submitter Id:ZF OE2 sc 2013 08 12T10:36:20Z 1677720|common name:zebrafish|sample description:RNA from OE|sample name:ZF OE2 sc 2013 08 12T10:36:20Z 1677720|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 10586 2#2,7941067,Illumina sequencing of library 7941067  constructed from sample accession ERS337051 for study accession ERP003756.  This is part of an Illumina multiplexed sequencing run 10586 2.  This submission includes reads tagged with the sequence CGATGT.,Illumina cDNA protocol,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP003756,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2013 11 25|ENA LAST UPDATE:2018 11 16,10586_2#2.cram,cram,10462424000.0,52312120.0,SC RUN 10586 2#2,0:100 1:100,A:3035632557;C:2208885147;G:2197231235;T:3006145790;N:14529271,100,100,,,3035632557,2208885147,2197231235,3006145790,14529271,ERX348125,ERS337051,ERA267455,SC,Wellcome Sanger Institute,2,0.91417,0.91329,0.15428,0.15477,0.66882,0.66896,0.52191,0.51898,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2013-11-25,Undetermined,Undetermined,Brain,Nervous System
10172,ERR375747,ERX348124,ERS337050,ERP003756,PRJEB4464,Zebrafish olfactory transcriptomics,Zebrafish_olfactory_transcriptomics-sc-2013-08-12T10:32:48Z-2742,Transcriptome Analysis,The olfactory gene repertoire is largely species specific  shaped by the nature and necessity of chemosensory information for survival in each species' niche. The relative expression of olfactory receptors can be quantified by RNA sequencing. We investigated the olfactory transcriptome of zebrafish because : i of its phylogenetic location in the vertebrate tree  ii it has only one olfactory organ yet it has representatives of all the mammalian chemosensory receptor families  iii its biology is well known  iv is a commonly used model organism  and it is suitable for subsequent functional analysis.,,,,,SAMEA2168446,SC,ArrayExpress Genotype:Wildtype|ArrayExpress OrganismPart:Olfactory epithelium|ArrayExpress Sex:male|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 11 25T10:18:08Z|ENA LAST UPDATE:2018 03 08T16:38:06Z|External Id:SAMEA2168446|INSDC center name:SC|INSDC first public:2013 11 25T10:18:08Z|INSDC last update:2018 03 08T16:38:06Z|INSDC status:public|Submitter Id:ZF OE1 sc 2013 08 12T10:36:17Z 1677719|common name:zebrafish|sample description:RNA from OE|sample name:ZF OE1 sc 2013 08 12T10:36:17Z 1677719|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 10586 2#1,7941066,Illumina sequencing of library 7941066  constructed from sample accession ERS337050 for study accession ERP003756.  This is part of an Illumina multiplexed sequencing run 10586 2.  This submission includes reads tagged with the sequence ATCACG.,Illumina cDNA protocol,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP003756,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2013 11 25|ENA LAST UPDATE:2018 11 16,10586_2#1.cram,cram,10377603200.0,51888016.0,SC RUN 10586 2#1,0:100 1:100,A:3021582125;C:2187474575;G:2170525378;T:2983668309;N:14352813,100,100,,,3021582125,2187474575,2170525378,2983668309,14352813,ERX348124,ERS337050,ERA267455,SC,Wellcome Sanger Institute,2,0.90978,0.90815,0.15565,0.15454,0.67322,0.67476,0.52923,0.51842,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2013-11-25,Undetermined,Undetermined,Brain,Nervous System
10173,ERR375746,ERX348123,ERS337052,ERP003756,PRJEB4464,Zebrafish olfactory transcriptomics,Zebrafish_olfactory_transcriptomics-sc-2013-08-12T10:32:48Z-2742,Transcriptome Analysis,The olfactory gene repertoire is largely species specific  shaped by the nature and necessity of chemosensory information for survival in each species' niche. The relative expression of olfactory receptors can be quantified by RNA sequencing. We investigated the olfactory transcriptome of zebrafish because : i of its phylogenetic location in the vertebrate tree  ii it has only one olfactory organ yet it has representatives of all the mammalian chemosensory receptor families  iii its biology is well known  iv is a commonly used model organism  and it is suitable for subsequent functional analysis.,,,,,SAMEA2168448,SC,ArrayExpress Genotype:Wildtype|ArrayExpress OrganismPart:Olfactory epithelium|ArrayExpress Sex:male|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 11 25T10:18:08Z|ENA LAST UPDATE:2018 03 08T16:38:06Z|External Id:SAMEA2168448|INSDC center name:SC|INSDC first public:2013 11 25T10:18:08Z|INSDC last update:2018 03 08T16:38:06Z|INSDC status:public|Submitter Id:ZF OE3 sc 2013 08 12T10:36:21Z 1677721|common name:zebrafish|sample description:RNA from OE|sample name:ZF OE3 sc 2013 08 12T10:36:21Z 1677721|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 10586 1#3,7941068,Illumina sequencing of library 7941068  constructed from sample accession ERS337052 for study accession ERP003756.  This is part of an Illumina multiplexed sequencing run 10586 1.  This submission includes reads tagged with the sequence TTAGGC.,Illumina cDNA protocol,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP003756,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2013 11 25|ENA LAST UPDATE:2018 11 16,10586_1#3.cram,cram,11455059800.0,57275299.0,SC RUN 10586 1#3,0:100 1:100,A:3406564785;C:2353388363;G:2329218644;T:3351844154;N:14043854,100,100,,,3406564785,2353388363,2329218644,3351844154,14043854,ERX348123,ERS337052,ERA267455,SC,Wellcome Sanger Institute,2,0.91087,0.90879,0.16647,0.16616,0.6832,0.68436,0.54098,0.53604,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2013-11-25,Undetermined,Undetermined,Brain,Nervous System
10174,ERR375745,ERX348122,ERS337051,ERP003756,PRJEB4464,Zebrafish olfactory transcriptomics,Zebrafish_olfactory_transcriptomics-sc-2013-08-12T10:32:48Z-2742,Transcriptome Analysis,The olfactory gene repertoire is largely species specific  shaped by the nature and necessity of chemosensory information for survival in each species' niche. The relative expression of olfactory receptors can be quantified by RNA sequencing. We investigated the olfactory transcriptome of zebrafish because : i of its phylogenetic location in the vertebrate tree  ii it has only one olfactory organ yet it has representatives of all the mammalian chemosensory receptor families  iii its biology is well known  iv is a commonly used model organism  and it is suitable for subsequent functional analysis.,,,,,SAMEA2168447,SC,ArrayExpress Genotype:Wildtype|ArrayExpress OrganismPart:Olfactory epithelium|ArrayExpress Sex:male|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 11 25T10:18:08Z|ENA LAST UPDATE:2018 03 08T16:38:13Z|External Id:SAMEA2168447|INSDC center name:SC|INSDC first public:2013 11 25T10:18:08Z|INSDC last update:2018 03 08T16:38:13Z|INSDC status:public|Submitter Id:ZF OE2 sc 2013 08 12T10:36:20Z 1677720|common name:zebrafish|sample description:RNA from OE|sample name:ZF OE2 sc 2013 08 12T10:36:20Z 1677720|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 10586 1#2,7941067,Illumina sequencing of library 7941067  constructed from sample accession ERS337051 for study accession ERP003756.  This is part of an Illumina multiplexed sequencing run 10586 1.  This submission includes reads tagged with the sequence CGATGT.,Illumina cDNA protocol,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP003756,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2013 11 25|ENA LAST UPDATE:2018 11 16,10586_1#2.cram,cram,10636681200.0,53183406.0,SC RUN 10586 1#2,0:100 1:100,A:3088549841;C:2244455059;G:2232424530;T:3058125106;N:13126664,100,100,,,3088549841,2244455059,2232424530,3058125106,13126664,ERX348122,ERS337051,ERA267455,SC,Wellcome Sanger Institute,2,0.9134,0.91251,0.15433,0.154,0.66849,0.66949,0.52089,0.53096,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2013-11-25,Undetermined,Undetermined,Brain,Nervous System
10175,ERR375744,ERX348121,ERS337050,ERP003756,PRJEB4464,Zebrafish olfactory transcriptomics,Zebrafish_olfactory_transcriptomics-sc-2013-08-12T10:32:48Z-2742,Transcriptome Analysis,The olfactory gene repertoire is largely species specific  shaped by the nature and necessity of chemosensory information for survival in each species' niche. The relative expression of olfactory receptors can be quantified by RNA sequencing. We investigated the olfactory transcriptome of zebrafish because : i of its phylogenetic location in the vertebrate tree  ii it has only one olfactory organ yet it has representatives of all the mammalian chemosensory receptor families  iii its biology is well known  iv is a commonly used model organism  and it is suitable for subsequent functional analysis.,,,,,SAMEA2168446,SC,ArrayExpress Genotype:Wildtype|ArrayExpress OrganismPart:Olfactory epithelium|ArrayExpress Sex:male|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 11 25T10:18:08Z|ENA LAST UPDATE:2018 03 08T16:38:06Z|External Id:SAMEA2168446|INSDC center name:SC|INSDC first public:2013 11 25T10:18:08Z|INSDC last update:2018 03 08T16:38:06Z|INSDC status:public|Submitter Id:ZF OE1 sc 2013 08 12T10:36:17Z 1677719|common name:zebrafish|sample description:RNA from OE|sample name:ZF OE1 sc 2013 08 12T10:36:17Z 1677719|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 10586 1#1,7941066,Illumina sequencing of library 7941066  constructed from sample accession ERS337050 for study accession ERP003756.  This is part of an Illumina multiplexed sequencing run 10586 1.  This submission includes reads tagged with the sequence ATCACG.,Illumina cDNA protocol,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP003756,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2013 11 25|ENA LAST UPDATE:2018 11 16,10586_1#1.cram,cram,10534775800.0,52673879.0,SC RUN 10586 1#1,0:100 1:100,A:3069581091;C:2219273001;G:2202030288;T:3030929981;N:12961439,100,100,,,3069581091,2219273001,2202030288,3030929981,12961439,ERX348121,ERS337050,ERA267455,SC,Wellcome Sanger Institute,2,0.91028,0.90986,0.15668,0.15607,0.67456,0.67472,0.52998,0.49845,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2013-11-25,Undetermined,Undetermined,Brain,Nervous System
19493,ERR14085264,ERX13487656,ERS22622156,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 1 Replicate 3,Day 1 Replicate 3,SAMEA117549392,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009436 Day 1 Replicate 3|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009436 Day 1 Replicate 3|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9517,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19137_1dpl3_R1.fastq.gz 19138_1dpl3_R2.fastq.gz,fastq fastq,9165478634.0,30349267.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9517,0:151 1:151,A:2646232308;C:1796801479;G:2124056448;T:2588021406;N:10366993,151,151,,,2646232308,1796801479,2124056448,2588021406,10366993,ERX13487656,ERS22622156,ERA31046063,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19494,ERR14085263,ERX13487655,ERS22622154,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 1 Replicate 2,Day 1 Replicate 2,SAMEA117549391,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009435 Day 1 Replicate 2|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009435 Day 1 Replicate 2|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9516,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19135_1dpl2_R1.fastq.gz 19136_1dpl2_R2.fastq.gz,fastq fastq,9442039362.0,31265031.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9516,0:151 1:151,A:2725446078;C:1808614751;G:2223018565;T:2675393466;N:9566502,151,151,,,2725446078,1808614751,2223018565,2675393466,9566502,ERX13487655,ERS22622154,ERA31046062,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19495,ERR14085271,ERX13487663,ERS22622318,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 7 Replicate 3,Day 7 Replicate 3,SAMEA117549418,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009442 Day 7 Replicate 3|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009442 Day 7 Replicate 3|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9523,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19149_7dpl5_R1.fastq.gz 19150_7dpl5_R2.fastq.gz,fastq fastq,9932206502.0,32888101.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9523,0:151 1:151,A:2877130874;C:2050638794;G:2165950578;T:2827301936;N:11184320,151,151,,,2877130874,2050638794,2165950578,2827301936,11184320,ERX13487663,ERS22622318,ERA31046070,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19496,ERR14085261,ERX13487653,ERS22622089,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 1 Replicate 1,Day 1 Replicate 1,SAMEA117549390,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009434 Day 1 Replicate 1|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009434 Day 1 Replicate 1|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9515,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19133_1dpl1_R1.fastq.gz 19134_1dpl1_R2.fastq.gz,fastq fastq,9389201442.0,31090071.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9515,0:151 1:151,A:2731715362;C:1816017173;G:2148260573;T:2683133097;N:10075237,151,151,,,2731715362,1816017173,2148260573,2683133097,10075237,ERX13487653,ERS22622089,ERA31046060,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19497,ERR14085274,ERX13487666,ERS22622321,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Control Replicate 3,Control Replicate 3,SAMEA117549421,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009445 Control Replicate 3|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009445 Control Replicate 3|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9526,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19155_C7_R1.fastq.gz 19156_C7_R2.fastq.gz,fastq fastq,9909221282.0,32811991.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9526,0:151 1:151,A:2850780132;C:1946613930;G:2307229799;T:2793332914;N:11264507,151,151,,,2850780132,1946613930,2307229799,2793332914,11264507,ERX13487666,ERS22622321,ERA31046073,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19498,ERR14085268,ERX13487660,ERS22622316,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 7 Replicate 1,Day 7 Replicate 1,SAMEA117549416,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009440 Day 7 Replicate 1|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009440 Day 7 Replicate 1|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9521,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19145_7dpl1_R1.fastq.gz 19146_7dpl1_R2.fastq.gz,fastq fastq,9208706914.0,30492407.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9521,0:151 1:151,A:2666375656;C:1820213036;G:2101314534;T:2611250093;N:9553595,151,151,,,2666375656,1820213036,2101314534,2611250093,9553595,ERX13487660,ERS22622316,ERA31046067,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19499,ERR14085276,ERX13487668,ERS22622315,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 4 Replicate 3,Day 4 Replicate 3,SAMEA117549415,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009439 Day 4 Replicate 3|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009439 Day 4 Replicate 3|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9520,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19143_4dpl4_R1.fastq.gz 19144_4dpl4_R2.fastq.gz,fastq fastq,9313489740.0,30839370.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9520,0:151 1:151,A:2667044089;C:1836529353;G:2193641971;T:2605720804;N:10553523,151,151,,,2667044089,1836529353,2193641971,2605720804,10553523,ERX13487668,ERS22622315,ERA31046075,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19500,ERR14085272,ERX13487664,ERS22622319,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Control Replicate 1,Control Replicate 1,SAMEA117549419,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009443 Control Replicate 1|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009443 Control Replicate 1|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9524,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19151_C5_R1.fastq.gz 19152_C5_R2.fastq.gz,fastq fastq,13295515874.0,44024887.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9524,0:151 1:151,A:3841326329;C:2750684077;G:2915049240;T:3777029141;N:11427087,151,151,,,3841326329,2750684077,2915049240,3777029141,11427087,ERX13487664,ERS22622319,ERA31046071,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19501,ERR14085265,ERX13487657,ERS22622177,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 4 Replicate 1,Day 4 Replicate 1,SAMEA117549413,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009437 Day 4 Replicate 1|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009437 Day 4 Replicate 1|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9518,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19139_4dpl2_R1.fastq.gz 19140_4dpl2_R2.fastq.gz,fastq fastq,12509373332.0,41421766.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9518,0:151 1:151,A:3636942004;C:2502487228;G:2790329437;T:3565530654;N:14084009,151,151,,,3636942004,2502487228,2790329437,3565530654,14084009,ERX13487657,ERS22622177,ERA31046064,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19502,ERR14085266,ERX13487658,ERS22622210,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 4 Replicate 2,Day 4 Replicate 2,SAMEA117549414,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009438 Day 4 Replicate 2|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009438 Day 4 Replicate 2|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9519,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19141_4dpl3_R1.fastq.gz 19142_4dpl3_R2.fastq.gz,fastq fastq,9691702460.0,32091730.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9519,0:151 1:151,A:2791714294;C:1934967288;G:2210755028;T:2743302083;N:10963767,151,151,,,2791714294,1934967288,2210755028,2743302083,10963767,ERX13487658,ERS22622210,ERA31046065,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19503,ERR14085269,ERX13487661,ERS22622317,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Day 7 Replicate 2,Day 7 Replicate 2,SAMEA117549417,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009441 Day 7 Replicate 2|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009441 Day 7 Replicate 2|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9522,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19147_7dpl4_R1.fastq.gz 19148_7dpl4_R2.fastq.gz,fastq fastq,9862024420.0,32655710.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9522,0:151 1:151,A:2841799545;C:2048598598;G:2166200803;T:2794242657;N:11182817,151,151,,,2841799545,2048598598,2166200803,2794242657,11182817,ERX13487661,ERS22622317,ERA31046068,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
19504,ERR14085273,ERX13487665,ERS22622320,ERP167301,PRJEB83711,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,"inda-STUDY-IIT-Delhi,Indraprastha Institute of Information Technology Shilpi Minocha, KSBS, IIT-Delhi New Delhi,India-2024-12-18 14:20:14.533-243",Other,Regeneration of damaged brain tissue is a complex biological process that varies significantly across species. Zebrafish possess a remarkable ability to regenerate central nervous system structures  making them an invaluable model for studying the molecular and cellular mechanisms underlying neuroregeneration. In this study  we employed a previously standardized telencephalic stab wound injury model to investigate transcriptional and cellular responses during zebrafish brain regeneration. This well characterized model allows for precise analysis of injury induced regenerative processes by comparing different temporal stages of recovery.  Using RNA sequencing at four key time points: control  1 day post lesion  4 dpl  and 7 dpl  we identified dynamic changes in gene expression and revealed critical signaling pathways associated with regeneration. Among these  the p38 MAPK signaling cascade emerged as a key regulator. Our findings underscore the multifaceted role of p38 MAPK  which modulates progenitor cell proliferation  differentiation  and neurogenesis during regeneration. These insights align with known roles of p38 MAPK in neural stem cell biology while highlighting its distinct contributions in a regenerative context.  This work provides a comprehensive overview of early transcriptional events and highlights novel molecular players involved in CNS repair  further establishing the zebrafish as a robust model for regenerative research. Our findings open avenues for applying these pathways to develop therapeutic strategies aimed at enhancing brain repair in humans.,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,,RNA seq Control Replicate 2,Control Replicate 2,SAMEA117549420,"Indian Institute of Technology Delhi,Indraprastha Institute of Information Technology",ENA first public:2024 12 19|INSDC center name:Indian Institute of Technology Delhi Indraprastha Institute of Information Technology|INSDC status:public|Submitter Id:SAMIN0009444 Control Replicate 2|broker name:IBDC|collection date:2022 12 23|common name:zebrafish|geographic location country and/or sea:India|geographic location region and locality:New Delhi India|sample name:SAMIN0009444 Control Replicate 2|scientific name:Danio rerio,,,,,,,,,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9525,1,1,NaN,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP167301,Illumina NovaSeq 6000 paired end sequencing; From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration,ENA FIRST PUBLIC:2024 12 19|ENA LAST UPDATE:2024 12 19,19153_C6_R1.fastq.gz 19154_C6_R2.fastq.gz,fastq fastq,11398526732.0,37743466.0,RUN From injury to recovery: Transcriptomic dynamics in Zebrafish brain regeneration 9525,0:151 1:151,A:3315198872;C:2268180334;G:2561003095;T:3244771542;N:9372889,151,151,,,3315198872,2268180334,2561003095,3244771542,9372889,ERX13487665,ERS22622320,ERA31046072,Indian Biological Data Centre|European Nucleotide Archive,Indian Biological Data Centre,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,India,2024-12-19,Undetermined,Undetermined,Brain,Nervous System
26484,SRR26034368,SRX21751589,SRS18859096,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X9 tp53mut control,GSM7774465,,source name:brain|tissue:brain|tumor status:control|age:1 year|genotype:tp53 mutant|geo loc name:missing|collection date:missing,19521X9 tp53mut control,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:control|age:1 year|genotype:tp53 mutant,GSM7774465,GSM7774465: 19521X9 tp53mut control; Danio rerio; RNA Seq,GSM7774465 r1,GSM7774465,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X9_220211_A00421_0418_AHGVTMDSX3_S180_L003_R2_001.fastq.gz 19521X9_220211_A00421_0418_AHGVTMDSX3_S180_L003_R1_001.fastq.gz,fastq fastq,13111362918.0,43415109.0,GSM7774465 r1,0:151 1:151,A:3716297698;C:2842682328;G:2964203082;T:3588045151;N:134659,151,151,,,3716297698,2842682328,2964203082,3588045151,134659,SRX21751589,SRS18859096,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.87652,0.87774,0.39216,0.39133,0.71102,0.71133,0.53264,0.53118,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Adult,Adult,Brain,Nervous System
26485,SRR26034369,SRX21751588,SRS18859094,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X8 tp53mut control,GSM7774464,,source name:brain|tissue:brain|tumor status:control|age:1 year|genotype:tp53 mutant|geo loc name:missing|collection date:missing,19521X8 tp53mut control,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:control|age:1 year|genotype:tp53 mutant,GSM7774464,GSM7774464: 19521X8 tp53mut control; Danio rerio; RNA Seq,GSM7774464 r1,GSM7774464,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X8_220211_A00421_0418_AHGVTMDSX3_S179_L003_R1_001.fastq.gz 19521X8_220211_A00421_0418_AHGVTMDSX3_S179_L003_R2_001.fastq.gz,fastq fastq,13892147074.0,46000487.0,GSM7774464 r1,0:151 1:151,A:3814725425;C:3130415629;G:3297762528;T:3649099178;N:144314,151,151,,,3814725425,3130415629,3297762528,3649099178,144314,SRX21751588,SRS18859094,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.89624,0.89685,0.34529,0.34496,0.70092,0.70335,0.53129,0.53612,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Adult,Adult,Brain,Nervous System
26486,SRR26034370,SRX21751587,SRS18859095,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X7 tp53mut control,GSM7774463,,source name:brain|tissue:brain|tumor status:control|age:1 year|genotype:tp53 mutant|geo loc name:missing|collection date:missing,19521X7 tp53mut control,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:control|age:1 year|genotype:tp53 mutant,GSM7774463,GSM7774463: 19521X7 tp53mut control; Danio rerio; RNA Seq,GSM7774463 r1,GSM7774463,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X7_220211_A00421_0418_AHGVTMDSX3_S178_L003_R1_001.fastq.gz 19521X7_220211_A00421_0418_AHGVTMDSX3_S178_L003_R2_001.fastq.gz,fastq fastq,15619675560.0,51720780.0,GSM7774463 r1,0:151 1:151,A:4385995044;C:3423475528;G:3608910971;T:4201133585;N:160432,151,151,,,4385995044,3423475528,3608910971,4201133585,160432,SRX21751587,SRS18859095,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.88198,0.88184,0.36824,0.36836,0.70138,0.70427,0.50325,0.50812,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Adult,Adult,Brain,Nervous System
26487,SRR26034371,SRX21751586,SRS18859093,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X6 tp53het tumor,GSM7774462,,source name:brain|tissue:brain|tumor status:tumor|age:7 wpf|genotype:tp53 heterozygous|geo loc name:missing|collection date:missing,19521X6 tp53het tumor,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:tumor|age:7 wpf|genotype:tp53 heterozygous,GSM7774462,GSM7774462: 19521X6 tp53het tumor; Danio rerio; RNA Seq,GSM7774462 r1,GSM7774462,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X6_220211_A00421_0418_AHGVTMDSX3_S177_L003_R1_001.fastq.gz 19521X6_220211_A00421_0418_AHGVTMDSX3_S177_L003_R2_001.fastq.gz,fastq fastq,13856710696.0,45883148.0,GSM7774462 r1,0:151 1:151,A:3910050404;C:3019565763;G:3183961340;T:3742992264;N:140925,151,151,,,3910050404,3019565763,3183961340,3742992264,140925,SRX21751586,SRS18859093,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.8794,0.87909,0.38495,0.38479,0.69753,0.70005,0.49322,0.49442,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Juvenile,Juvenile,Brain,Nervous System
26488,SRR26034372,SRX21751585,SRS18859092,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X5 tp53het tumor,GSM7774461,,source name:brain|tissue:brain|tumor status:tumor|age:7 wpf|genotype:tp53 heterozygous|geo loc name:missing|collection date:missing,19521X5 tp53het tumor,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:tumor|age:7 wpf|genotype:tp53 heterozygous,GSM7774461,GSM7774461: 19521X5 tp53het tumor; Danio rerio; RNA Seq,GSM7774461 r1,GSM7774461,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X5_220211_A00421_0418_AHGVTMDSX3_S176_L003_R1_001.fastq.gz 19521X5_220211_A00421_0418_AHGVTMDSX3_S176_L003_R2_001.fastq.gz,fastq fastq,18644081740.0,61735370.0,GSM7774461 r1,0:151 1:151,A:5198614433;C:4108657498;G:4307546758;T:5029072002;N:191049,151,151,,,5198614433,4108657498,4307546758,5029072002,191049,SRX21751585,SRS18859092,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.88544,0.88842,0.35896,0.35863,0.69193,0.69248,0.49682,0.50274,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Juvenile,Juvenile,Brain,Nervous System
26489,SRR26034373,SRX21751584,SRS18859091,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X4 tp53het tumor,GSM7774460,,source name:brain|tissue:brain|tumor status:tumor|age:7 wpf|genotype:tp53 heterozygous|geo loc name:missing|collection date:missing,19521X4 tp53het tumor,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:tumor|age:7 wpf|genotype:tp53 heterozygous,GSM7774460,GSM7774460: 19521X4 tp53het tumor; Danio rerio; RNA Seq,GSM7774460 r1,GSM7774460,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X4_220211_A00421_0418_AHGVTMDSX3_S175_L003_R1_001.fastq.gz 19521X4_220211_A00421_0418_AHGVTMDSX3_S175_L003_R2_001.fastq.gz,fastq fastq,14417628886.0,47740493.0,GSM7774460 r1,0:151 1:151,A:4016299076;C:3183962020;G:3341158152;T:3876059438;N:150200,151,151,,,4016299076,3183962020,3341158152,3876059438,150200,SRX21751584,SRS18859091,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.89118,0.89184,0.33812,0.33641,0.68947,0.68954,0.49867,0.50026,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Juvenile,Juvenile,Brain,Nervous System
26490,SRR26034374,SRX21751583,SRS18859090,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X3 tp53mut tumor,GSM7774459,,source name:brain|tissue:brain|tumor status:tumor|age:4 wpf|genotype:tp53 mutant|geo loc name:missing|collection date:missing,19521X3 tp53mut tumor,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:tumor|age:4 wpf|genotype:tp53 mutant,GSM7774459,GSM7774459: 19521X3 tp53mut tumor; Danio rerio; RNA Seq,GSM7774459 r1,GSM7774459,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X3_220211_A00421_0418_AHGVTMDSX3_S174_L003_R1_001.fastq.gz 19521X3_220211_A00421_0418_AHGVTMDSX3_S174_L003_R2_001.fastq.gz,fastq fastq,14792155696.0,48980648.0,GSM7774459 r1,0:151 1:151,A:4164614124;C:3233958816;G:3393302319;T:4000129008;N:151429,151,151,,,4164614124,3233958816,3393302319,4000129008,151429,SRX21751583,SRS18859090,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.89303,0.8944,0.32883,0.32864,0.68024,0.68091,0.48335,0.48739,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Larval,Larval,Brain,Nervous System
26491,SRR26034375,SRX21751582,SRS18859089,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X2 tp53mut tumor,GSM7774458,,source name:brain|tissue:brain|tumor status:tumor|age:8 wpf|genotype:tp53 mutant|geo loc name:missing|collection date:missing,19521X2 tp53mut tumor,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:tumor|age:8 wpf|genotype:tp53 mutant,GSM7774458,GSM7774458: 19521X2 tp53mut tumor; Danio rerio; RNA Seq,GSM7774458 r1,GSM7774458,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X2_220211_A00421_0418_AHGVTMDSX3_S173_L003_R1_001.fastq.gz 19521X2_220211_A00421_0418_AHGVTMDSX3_S173_L003_R2_001.fastq.gz,fastq fastq,14064076580.0,46569790.0,GSM7774458 r1,0:151 1:151,A:3935649548;C:3089754695;G:3257286821;T:3781242542;N:142974,151,151,,,3935649548,3089754695,3257286821,3781242542,142974,SRX21751582,SRS18859089,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.89595,0.89655,0.35454,0.35431,0.68927,0.69077,0.47888,0.47831,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Juvenile,Juvenile,Brain,Nervous System
26492,SRR26034376,SRX21751581,SRS18859088,SRP459779,PRJNA1015242,Gene expression data from zebrafish SHH medulloblastoma brains and normal brains.,GSE242897,Transcriptome Analysis,Zebrafish SHH MB tumors were generated by CRISPR Cas9 mediated mutation of ptch1 in the context of tp53 heterozygous and tp53 mutant animals. Gene expression of the entire brain for ptch1 crispant animals and tp53 mutant control animals was analyzed by RNA seq. Overall design: Whole brains were collected from 3 individual tp53 mutant control animals  3 individual tp53 mutant and ptch1 crispant animals  and 3 individual tp53 heterozygous and ptch1 crispant animals.,,pubmed:39078737,,19521X1 tp53mut tumor,GSM7774457,,source name:brain|tissue:brain|tumor status:tumor|age:7 wpf|genotype:tp53 mutant|geo loc name:missing|collection date:missing,19521X1 tp53mut tumor,The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.9a Differentially expressed genes were found using DESeq2 version 1.32.0 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts.,brain,Brains were frozen at  80C in RNA stabilization solution QIAGEN.,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,Zebrafish were maintained in the animal facility in accordance with the Utah Institutional Animal Care and Use Committee.,tissue:brain|tumor status:tumor|age:7 wpf|genotype:tp53 mutant,GSM7774457,GSM7774457: 19521X1 tp53mut tumor; Danio rerio; RNA Seq,GSM7774457 r1,GSM7774457,1,Homogenization and total RNA isolation performed according to QIAGEN miRNeasy micro kit Qiagen 217084. Homogenization was performed with a 20 25.5 gauge needle 10 times. Samples were Dnase treated. Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold Illumina 20020598.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP459779,,loader:fastq load.py,19521X1_220211_A00421_0418_AHGVTMDSX3_S172_L003_R1_001.fastq.gz 19521X1_220211_A00421_0418_AHGVTMDSX3_S172_L003_R2_001.fastq.gz,fastq fastq,16911500190.0,55998345.0,GSM7774457 r1,0:151 1:151,A:4750241986;C:3698380612;G:3890633834;T:4572071732;N:172026,151,151,,,4750241986,3698380612,3890633834,4572071732,172026,SRX21751581,SRS18859088,SRA1710210,Huntsman Cancer Institute,"Oncological Sciences, University of Utah",2,0.89695,0.89831,0.3562,0.35542,0.68392,0.68369,0.48172,0.48641,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United States,2023-09-11,Juvenile,Juvenile,Brain,Nervous System
28482,SRR26266492,SRX21975998,SRS19051834,SRP464334,PRJNA1023540,ABCA7 dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling,GSE244550,Transcriptome Analysis,Genetic variants in ABCA7  an Alzheimer's disease AD associated gene  elevate AD risk  yet its functional relevance to the etiology is unclear. We generated a CRISPR Cas9 mediated abca7 knockout zebrafish to explore ABCA7's role in AD. Single cell transcriptomics in heterozygous abca7+/  knockout combined with Aß42 toxicity revealed that ABCA7 is crucial for neuropeptide Y NPY  brain derived neurotrophic factor BDNF  and nerve growth factor receptor NGFR expressions  which are crucial for synaptic integrity  astroglial proliferation  and microglial prevalence. Impaired NPY induction decreased BDNF and synaptic density  which are rescuable with ectopic NPY. In induced pluripotent stem cell derived human neurons exposed to Aß42  ABCA7 /  suppresses NPY. Clinical data showed reduced NPY in AD correlated with elevated Braak stages  genetic variants in NPY associated with AD  and epigenetic changes in NPY  NGFR  and BDNF promoters linked to ABCA7 variants. Therefore  ABCA7 dependent NPY signaling via BDNF NGFR maintains synaptic integrity  implicating its impairment in increased AD risk through reduced brain resilience. Overall design: WT and abca7+/  zebrafish injected with Amyloid beta 42 peptide as described Bhatatrai et al. 2016 and cells were dissociated  sorted by FACS as described Cosacak et al. 2019. The single cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome GRChZ 11  v 105 was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data,,pubmed:39216475,,telencephalon  abca7 k/o   Ab42 injected,GSM7819018,,source name:telencephalon|tissue:telencephalon|genotype:abca7 +/ |treatment:Ab42|geo loc name:missing|collection date:missing,telencephalon  abca7 k/o   Ab42 injected,The demultiplexing  barcoded processing  gene counting and aggregation were made using the 10x genomics' cellranger software version 6.1.2 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/output/metrics The fastq files were aligned to zebrafish genome GRCz11 and ensemble transcripts from Ensembl Release 105 by using STAR. The BAM files were as input for Cell Ranger 10X genomics to generate processed data files that include gene names row names and cell names column names and counts. Further analysis done by using Seurat package in R. Assembly: GRCz11 Supplementary files format and content: filtered bc matrix outputs from Cell Ranger,telencephalon,WT AB and abca7 +/  knock out lines were injected with Amyloid beta 42 peptide  and PBS as control as descibed Bhatatrai et al. 2016.,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,Zebrafish were kept in the re circulating system on a 14/10 h light/dark cycle  pH7.5  at 28°C ±1°C in groups of 20 animals per 2.8L.,tissue:telencephalon|genotype:abca7 +/ |treatment:Ab42,GSM7819018,GSM7819018: telencephalon  abca7 k/o   Ab42 injected; Danio rerio; RNA Seq,GSM7819018 r1,GSM7819018,1,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP464334,,loader:fastq load.py,CP021_S12_L001_I1_001.fastq.gz CP021_S12_L001_I2_001.fastq.gz CP021_S12_L001_R1_001.fastq.gz CP021_S12_L001_R2_001.fastq.gz,fastq fastq fastq fastq,46637839920.0,210080360.0,GSM7819018 r1,0:10 1:10 2:101 3:101,A:12160340644;C:7050177030;G:7302497483;T:15922643298;N:574265,10,10,101,101,12160340644,7050177030,7302497483,15922643298,574265,SRX21975998,SRS19051834,SRA1725563,"Neurology &amp; TAUB Institute, Columbia University","Neurology & TAUB Institute, Columbia University",2,0.00333,0.89749,0.00051,0.26345,0.99821,0.73539,0.54736,0.5015,101,101,T,B,mate1 technical by mapping diff,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2023-10-03,Undetermined,Undetermined,Brain,Nervous System
28483,SRR26266493,SRX21975998,SRS19051834,SRP464334,PRJNA1023540,ABCA7 dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling,GSE244550,Transcriptome Analysis,Genetic variants in ABCA7  an Alzheimer's disease AD associated gene  elevate AD risk  yet its functional relevance to the etiology is unclear. We generated a CRISPR Cas9 mediated abca7 knockout zebrafish to explore ABCA7's role in AD. Single cell transcriptomics in heterozygous abca7+/  knockout combined with Aß42 toxicity revealed that ABCA7 is crucial for neuropeptide Y NPY  brain derived neurotrophic factor BDNF  and nerve growth factor receptor NGFR expressions  which are crucial for synaptic integrity  astroglial proliferation  and microglial prevalence. Impaired NPY induction decreased BDNF and synaptic density  which are rescuable with ectopic NPY. In induced pluripotent stem cell derived human neurons exposed to Aß42  ABCA7 /  suppresses NPY. Clinical data showed reduced NPY in AD correlated with elevated Braak stages  genetic variants in NPY associated with AD  and epigenetic changes in NPY  NGFR  and BDNF promoters linked to ABCA7 variants. Therefore  ABCA7 dependent NPY signaling via BDNF NGFR maintains synaptic integrity  implicating its impairment in increased AD risk through reduced brain resilience. Overall design: WT and abca7+/  zebrafish injected with Amyloid beta 42 peptide as described Bhatatrai et al. 2016 and cells were dissociated  sorted by FACS as described Cosacak et al. 2019. The single cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome GRChZ 11  v 105 was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data,,pubmed:39216475,,telencephalon  abca7 k/o   Ab42 injected,GSM7819018,,source name:telencephalon|tissue:telencephalon|genotype:abca7 +/ |treatment:Ab42|geo loc name:missing|collection date:missing,telencephalon  abca7 k/o   Ab42 injected,The demultiplexing  barcoded processing  gene counting and aggregation were made using the 10x genomics' cellranger software version 6.1.2 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/output/metrics The fastq files were aligned to zebrafish genome GRCz11 and ensemble transcripts from Ensembl Release 105 by using STAR. The BAM files were as input for Cell Ranger 10X genomics to generate processed data files that include gene names row names and cell names column names and counts. Further analysis done by using Seurat package in R. Assembly: GRCz11 Supplementary files format and content: filtered bc matrix outputs from Cell Ranger,telencephalon,WT AB and abca7 +/  knock out lines were injected with Amyloid beta 42 peptide  and PBS as control as descibed Bhatatrai et al. 2016.,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,Zebrafish were kept in the re circulating system on a 14/10 h light/dark cycle  pH7.5  at 28°C ±1°C in groups of 20 animals per 2.8L.,tissue:telencephalon|genotype:abca7 +/ |treatment:Ab42,GSM7819018,GSM7819018: telencephalon  abca7 k/o   Ab42 injected; Danio rerio; RNA Seq,GSM7819018 r1,GSM7819018,1,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP464334,,loader:fastq load.py,CP021_S12_L002_I1_001.fastq.gz CP021_S12_L002_I2_001.fastq.gz CP021_S12_L002_R1_001.fastq.gz CP021_S12_L002_R2_001.fastq.gz,fastq fastq fastq fastq,47673315186.0,214744663.0,GSM7819018 r2,0:10 1:10 2:101 3:101,A:12499321052;C:7231866994;G:7443419880;T:16203206561;N:607439,10,10,101,101,12499321052,7231866994,7443419880,16203206561,607439,SRX21975998,SRS19051834,SRA1725563,"Neurology &amp; TAUB Institute, Columbia University","Neurology & TAUB Institute, Columbia University",2,0.00305,0.89832,0.00064,0.26179,0.99851,0.73434,0.53932,0.50016,101,101,T,B,mate1 technical by mapping diff,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2023-10-03,Undetermined,Undetermined,Brain,Nervous System
28484,SRR26266494,SRX21975997,SRS19051833,SRP464334,PRJNA1023540,ABCA7 dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling,GSE244550,Transcriptome Analysis,Genetic variants in ABCA7  an Alzheimer's disease AD associated gene  elevate AD risk  yet its functional relevance to the etiology is unclear. We generated a CRISPR Cas9 mediated abca7 knockout zebrafish to explore ABCA7's role in AD. Single cell transcriptomics in heterozygous abca7+/  knockout combined with Aß42 toxicity revealed that ABCA7 is crucial for neuropeptide Y NPY  brain derived neurotrophic factor BDNF  and nerve growth factor receptor NGFR expressions  which are crucial for synaptic integrity  astroglial proliferation  and microglial prevalence. Impaired NPY induction decreased BDNF and synaptic density  which are rescuable with ectopic NPY. In induced pluripotent stem cell derived human neurons exposed to Aß42  ABCA7 /  suppresses NPY. Clinical data showed reduced NPY in AD correlated with elevated Braak stages  genetic variants in NPY associated with AD  and epigenetic changes in NPY  NGFR  and BDNF promoters linked to ABCA7 variants. Therefore  ABCA7 dependent NPY signaling via BDNF NGFR maintains synaptic integrity  implicating its impairment in increased AD risk through reduced brain resilience. Overall design: WT and abca7+/  zebrafish injected with Amyloid beta 42 peptide as described Bhatatrai et al. 2016 and cells were dissociated  sorted by FACS as described Cosacak et al. 2019. The single cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome GRChZ 11  v 105 was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data,,pubmed:39216475,,telencephalon  abca7 k/o   PBS injected,GSM7819017,,source name:telencephalon|tissue:telencephalon|genotype:abca7 +/ |treatment:PBS|geo loc name:missing|collection date:missing,telencephalon  abca7 k/o   PBS injected,The demultiplexing  barcoded processing  gene counting and aggregation were made using the 10x genomics' cellranger software version 6.1.2 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/output/metrics The fastq files were aligned to zebrafish genome GRCz11 and ensemble transcripts from Ensembl Release 105 by using STAR. The BAM files were as input for Cell Ranger 10X genomics to generate processed data files that include gene names row names and cell names column names and counts. Further analysis done by using Seurat package in R. Assembly: GRCz11 Supplementary files format and content: filtered bc matrix outputs from Cell Ranger,telencephalon,WT AB and abca7 +/  knock out lines were injected with Amyloid beta 42 peptide  and PBS as control as descibed Bhatatrai et al. 2016.,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,Zebrafish were kept in the re circulating system on a 14/10 h light/dark cycle  pH7.5  at 28°C ±1°C in groups of 20 animals per 2.8L.,tissue:telencephalon|genotype:abca7 +/ |treatment:PBS,GSM7819017,GSM7819017: telencephalon  abca7 k/o   PBS injected; Danio rerio; RNA Seq,GSM7819017 r1,GSM7819017,1,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP464334,,loader:fastq load.py,CP020_S11_L001_I1_001.fastq.gz CP020_S11_L001_I2_001.fastq.gz CP020_S11_L001_R1_001.fastq.gz CP020_S11_L001_R2_001.fastq.gz,fastq fastq fastq fastq,45135900918.0,203314869.0,GSM7819017 r1,0:10 1:10 2:101 3:101,A:11807801920;C:6876592307;G:7115059785;T:15269592915;N:556611,10,10,101,101,11807801920,6876592307,7115059785,15269592915,556611,SRX21975997,SRS19051833,SRA1725563,"Neurology &amp; TAUB Institute, Columbia University","Neurology & TAUB Institute, Columbia University",2,0.00293,0.90111,0.00093,0.28713,0.99882,0.73687,0.58461,0.50013,101,101,T,B,mate1 technical by mapping diff,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2023-10-03,Undetermined,Undetermined,Brain,Nervous System
28485,SRR26266495,SRX21975997,SRS19051833,SRP464334,PRJNA1023540,ABCA7 dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling,GSE244550,Transcriptome Analysis,Genetic variants in ABCA7  an Alzheimer's disease AD associated gene  elevate AD risk  yet its functional relevance to the etiology is unclear. We generated a CRISPR Cas9 mediated abca7 knockout zebrafish to explore ABCA7's role in AD. Single cell transcriptomics in heterozygous abca7+/  knockout combined with Aß42 toxicity revealed that ABCA7 is crucial for neuropeptide Y NPY  brain derived neurotrophic factor BDNF  and nerve growth factor receptor NGFR expressions  which are crucial for synaptic integrity  astroglial proliferation  and microglial prevalence. Impaired NPY induction decreased BDNF and synaptic density  which are rescuable with ectopic NPY. In induced pluripotent stem cell derived human neurons exposed to Aß42  ABCA7 /  suppresses NPY. Clinical data showed reduced NPY in AD correlated with elevated Braak stages  genetic variants in NPY associated with AD  and epigenetic changes in NPY  NGFR  and BDNF promoters linked to ABCA7 variants. Therefore  ABCA7 dependent NPY signaling via BDNF NGFR maintains synaptic integrity  implicating its impairment in increased AD risk through reduced brain resilience. Overall design: WT and abca7+/  zebrafish injected with Amyloid beta 42 peptide as described Bhatatrai et al. 2016 and cells were dissociated  sorted by FACS as described Cosacak et al. 2019. The single cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome GRChZ 11  v 105 was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data,,pubmed:39216475,,telencephalon  abca7 k/o   PBS injected,GSM7819017,,source name:telencephalon|tissue:telencephalon|genotype:abca7 +/ |treatment:PBS|geo loc name:missing|collection date:missing,telencephalon  abca7 k/o   PBS injected,The demultiplexing  barcoded processing  gene counting and aggregation were made using the 10x genomics' cellranger software version 6.1.2 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/output/metrics The fastq files were aligned to zebrafish genome GRCz11 and ensemble transcripts from Ensembl Release 105 by using STAR. The BAM files were as input for Cell Ranger 10X genomics to generate processed data files that include gene names row names and cell names column names and counts. Further analysis done by using Seurat package in R. Assembly: GRCz11 Supplementary files format and content: filtered bc matrix outputs from Cell Ranger,telencephalon,WT AB and abca7 +/  knock out lines were injected with Amyloid beta 42 peptide  and PBS as control as descibed Bhatatrai et al. 2016.,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,Zebrafish were kept in the re circulating system on a 14/10 h light/dark cycle  pH7.5  at 28°C ±1°C in groups of 20 animals per 2.8L.,tissue:telencephalon|genotype:abca7 +/ |treatment:PBS,GSM7819017,GSM7819017: telencephalon  abca7 k/o   PBS injected; Danio rerio; RNA Seq,GSM7819017 r1,GSM7819017,1,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP464334,,loader:fastq load.py,CP020_S11_L002_I1_001.fastq.gz CP020_S11_L002_I2_001.fastq.gz CP020_S11_L002_R1_001.fastq.gz CP020_S11_L002_R2_001.fastq.gz,fastq fastq fastq fastq,46689428280.0,210312740.0,GSM7819017 r2,0:10 1:10 2:101 3:101,A:12281435026;C:7136662243;G:7338982062;T:15725501987;N:592162,10,10,101,101,12281435026,7136662243,7338982062,15725501987,592162,SRX21975997,SRS19051833,SRA1725563,"Neurology &amp; TAUB Institute, Columbia University","Neurology & TAUB Institute, Columbia University",2,0.00317,0.90041,0.00069,0.28878,0.99819,0.73657,0.60869,0.49401,101,101,T,B,mate1 technical by mapping diff,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2023-10-03,Undetermined,Undetermined,Brain,Nervous System
28486,SRR26266496,SRX21975996,SRS19051832,SRP464334,PRJNA1023540,ABCA7 dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling,GSE244550,Transcriptome Analysis,Genetic variants in ABCA7  an Alzheimer's disease AD associated gene  elevate AD risk  yet its functional relevance to the etiology is unclear. We generated a CRISPR Cas9 mediated abca7 knockout zebrafish to explore ABCA7's role in AD. Single cell transcriptomics in heterozygous abca7+/  knockout combined with Aß42 toxicity revealed that ABCA7 is crucial for neuropeptide Y NPY  brain derived neurotrophic factor BDNF  and nerve growth factor receptor NGFR expressions  which are crucial for synaptic integrity  astroglial proliferation  and microglial prevalence. Impaired NPY induction decreased BDNF and synaptic density  which are rescuable with ectopic NPY. In induced pluripotent stem cell derived human neurons exposed to Aß42  ABCA7 /  suppresses NPY. Clinical data showed reduced NPY in AD correlated with elevated Braak stages  genetic variants in NPY associated with AD  and epigenetic changes in NPY  NGFR  and BDNF promoters linked to ABCA7 variants. Therefore  ABCA7 dependent NPY signaling via BDNF NGFR maintains synaptic integrity  implicating its impairment in increased AD risk through reduced brain resilience. Overall design: WT and abca7+/  zebrafish injected with Amyloid beta 42 peptide as described Bhatatrai et al. 2016 and cells were dissociated  sorted by FACS as described Cosacak et al. 2019. The single cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome GRChZ 11  v 105 was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data,,pubmed:39216475,,telencephalon  WT Ab42 injected,GSM7819016,,source name:telencephalon|tissue:telencephalon|genotype:WT AB|treatment:Ab42|geo loc name:missing|collection date:missing,telencephalon  WT Ab42 injected,The demultiplexing  barcoded processing  gene counting and aggregation were made using the 10x genomics' cellranger software version 6.1.2 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/output/metrics The fastq files were aligned to zebrafish genome GRCz11 and ensemble transcripts from Ensembl Release 105 by using STAR. The BAM files were as input for Cell Ranger 10X genomics to generate processed data files that include gene names row names and cell names column names and counts. Further analysis done by using Seurat package in R. Assembly: GRCz11 Supplementary files format and content: filtered bc matrix outputs from Cell Ranger,telencephalon,WT AB and abca7 +/  knock out lines were injected with Amyloid beta 42 peptide  and PBS as control as descibed Bhatatrai et al. 2016.,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,Zebrafish were kept in the re circulating system on a 14/10 h light/dark cycle  pH7.5  at 28°C ±1°C in groups of 20 animals per 2.8L.,tissue:telencephalon|genotype:WT AB|treatment:Ab42,GSM7819016,GSM7819016: telencephalon  WT Ab42 injected; Danio rerio; RNA Seq,GSM7819016 r1,GSM7819016,1,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP464334,,loader:fastq load.py,CP019_S10_L001_I1_001.fastq.gz CP019_S10_L001_I2_001.fastq.gz CP019_S10_L001_R1_001.fastq.gz CP019_S10_L001_R2_001.fastq.gz,fastq fastq fastq fastq,48132368784.0,216812472.0,GSM7819016 r1,0:10 1:10 2:101 3:101,A:12479944041;C:7377568938;G:7622029272;T:16315981376;N:595717,10,10,101,101,12479944041,7377568938,7622029272,16315981376,595717,SRX21975996,SRS19051832,SRA1725563,"Neurology &amp; TAUB Institute, Columbia University","Neurology & TAUB Institute, Columbia University",2,0.00237,0.90638,0.00049,0.23035,0.99876,0.73612,0.50793,0.50292,101,101,T,B,mate1 technical by mapping diff,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2023-10-03,Undetermined,Undetermined,Brain,Nervous System
28487,SRR26266497,SRX21975996,SRS19051832,SRP464334,PRJNA1023540,ABCA7 dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling,GSE244550,Transcriptome Analysis,Genetic variants in ABCA7  an Alzheimer's disease AD associated gene  elevate AD risk  yet its functional relevance to the etiology is unclear. We generated a CRISPR Cas9 mediated abca7 knockout zebrafish to explore ABCA7's role in AD. Single cell transcriptomics in heterozygous abca7+/  knockout combined with Aß42 toxicity revealed that ABCA7 is crucial for neuropeptide Y NPY  brain derived neurotrophic factor BDNF  and nerve growth factor receptor NGFR expressions  which are crucial for synaptic integrity  astroglial proliferation  and microglial prevalence. Impaired NPY induction decreased BDNF and synaptic density  which are rescuable with ectopic NPY. In induced pluripotent stem cell derived human neurons exposed to Aß42  ABCA7 /  suppresses NPY. Clinical data showed reduced NPY in AD correlated with elevated Braak stages  genetic variants in NPY associated with AD  and epigenetic changes in NPY  NGFR  and BDNF promoters linked to ABCA7 variants. Therefore  ABCA7 dependent NPY signaling via BDNF NGFR maintains synaptic integrity  implicating its impairment in increased AD risk through reduced brain resilience. Overall design: WT and abca7+/  zebrafish injected with Amyloid beta 42 peptide as described Bhatatrai et al. 2016 and cells were dissociated  sorted by FACS as described Cosacak et al. 2019. The single cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome GRChZ 11  v 105 was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data,,pubmed:39216475,,telencephalon  WT Ab42 injected,GSM7819016,,source name:telencephalon|tissue:telencephalon|genotype:WT AB|treatment:Ab42|geo loc name:missing|collection date:missing,telencephalon  WT Ab42 injected,The demultiplexing  barcoded processing  gene counting and aggregation were made using the 10x genomics' cellranger software version 6.1.2 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/output/metrics The fastq files were aligned to zebrafish genome GRCz11 and ensemble transcripts from Ensembl Release 105 by using STAR. The BAM files were as input for Cell Ranger 10X genomics to generate processed data files that include gene names row names and cell names column names and counts. Further analysis done by using Seurat package in R. Assembly: GRCz11 Supplementary files format and content: filtered bc matrix outputs from Cell Ranger,telencephalon,WT AB and abca7 +/  knock out lines were injected with Amyloid beta 42 peptide  and PBS as control as descibed Bhatatrai et al. 2016.,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,Zebrafish were kept in the re circulating system on a 14/10 h light/dark cycle  pH7.5  at 28°C ±1°C in groups of 20 animals per 2.8L.,tissue:telencephalon|genotype:WT AB|treatment:Ab42,GSM7819016,GSM7819016: telencephalon  WT Ab42 injected; Danio rerio; RNA Seq,GSM7819016 r1,GSM7819016,1,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP464334,,loader:fastq load.py,CP019_S10_L002_I1_001.fastq.gz CP019_S10_L002_I2_001.fastq.gz CP019_S10_L002_R1_001.fastq.gz CP019_S10_L002_R2_001.fastq.gz,fastq fastq fastq fastq,49675882836.0,223765238.0,GSM7819016 r2,0:10 1:10 2:101 3:101,A:12950211284;C:7640502677;G:7844712651;T:16764526831;N:624633,10,10,101,101,12950211284,7640502677,7844712651,16764526831,624633,SRX21975996,SRS19051832,SRA1725563,"Neurology &amp; TAUB Institute, Columbia University","Neurology & TAUB Institute, Columbia University",2,0.0034,0.9058,0.00062,0.22634,0.99825,0.73423,0.45714,0.49934,101,101,T,B,mate1 technical by mapping diff,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2023-10-03,Undetermined,Undetermined,Brain,Nervous System
28488,SRR26266498,SRX21975995,SRS19051831,SRP464334,PRJNA1023540,ABCA7 dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling,GSE244550,Transcriptome Analysis,Genetic variants in ABCA7  an Alzheimer's disease AD associated gene  elevate AD risk  yet its functional relevance to the etiology is unclear. We generated a CRISPR Cas9 mediated abca7 knockout zebrafish to explore ABCA7's role in AD. Single cell transcriptomics in heterozygous abca7+/  knockout combined with Aß42 toxicity revealed that ABCA7 is crucial for neuropeptide Y NPY  brain derived neurotrophic factor BDNF  and nerve growth factor receptor NGFR expressions  which are crucial for synaptic integrity  astroglial proliferation  and microglial prevalence. Impaired NPY induction decreased BDNF and synaptic density  which are rescuable with ectopic NPY. In induced pluripotent stem cell derived human neurons exposed to Aß42  ABCA7 /  suppresses NPY. Clinical data showed reduced NPY in AD correlated with elevated Braak stages  genetic variants in NPY associated with AD  and epigenetic changes in NPY  NGFR  and BDNF promoters linked to ABCA7 variants. Therefore  ABCA7 dependent NPY signaling via BDNF NGFR maintains synaptic integrity  implicating its impairment in increased AD risk through reduced brain resilience. Overall design: WT and abca7+/  zebrafish injected with Amyloid beta 42 peptide as described Bhatatrai et al. 2016 and cells were dissociated  sorted by FACS as described Cosacak et al. 2019. The single cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome GRChZ 11  v 105 was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data,,pubmed:39216475,,telencephalon  WT  PBS injected,GSM7819015,,source name:telencephalon|tissue:telencephalon|genotype:WT AB|treatment:PBS|geo loc name:missing|collection date:missing,telencephalon  WT  PBS injected,The demultiplexing  barcoded processing  gene counting and aggregation were made using the 10x genomics' cellranger software version 6.1.2 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/output/metrics The fastq files were aligned to zebrafish genome GRCz11 and ensemble transcripts from Ensembl Release 105 by using STAR. The BAM files were as input for Cell Ranger 10X genomics to generate processed data files that include gene names row names and cell names column names and counts. Further analysis done by using Seurat package in R. Assembly: GRCz11 Supplementary files format and content: filtered bc matrix outputs from Cell Ranger,telencephalon,WT AB and abca7 +/  knock out lines were injected with Amyloid beta 42 peptide  and PBS as control as descibed Bhatatrai et al. 2016.,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,Zebrafish were kept in the re circulating system on a 14/10 h light/dark cycle  pH7.5  at 28°C ±1°C in groups of 20 animals per 2.8L.,tissue:telencephalon|genotype:WT AB|treatment:PBS,GSM7819015,GSM7819015: telencephalon  WT  PBS injected; Danio rerio; RNA Seq,GSM7819015 r1,GSM7819015,1,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP464334,,loader:fastq load.py,CP018_S9_L001_I1_001.fastq.gz CP018_S9_L001_I2_001.fastq.gz CP018_S9_L001_R1_001.fastq.gz CP018_S9_L001_R2_001.fastq.gz,fastq fastq fastq fastq,45948585420.0,206975610.0,GSM7819015 r1,0:10 1:10 2:101 3:101,A:12050390597;C:6986598890;G:7200535422;T:15570981408;N:566903,10,10,101,101,12050390597,6986598890,7200535422,15570981408,566903,SRX21975995,SRS19051831,SRA1725563,"Neurology &amp; TAUB Institute, Columbia University","Neurology & TAUB Institute, Columbia University",2,0.00353,0.89238,0.00096,0.30364,0.99841,0.73456,0.59523,0.49758,101,101,T,B,mate1 technical by mapping diff,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2023-10-03,Undetermined,Undetermined,Brain,Nervous System
28489,SRR26266499,SRX21975995,SRS19051831,SRP464334,PRJNA1023540,ABCA7 dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling,GSE244550,Transcriptome Analysis,Genetic variants in ABCA7  an Alzheimer's disease AD associated gene  elevate AD risk  yet its functional relevance to the etiology is unclear. We generated a CRISPR Cas9 mediated abca7 knockout zebrafish to explore ABCA7's role in AD. Single cell transcriptomics in heterozygous abca7+/  knockout combined with Aß42 toxicity revealed that ABCA7 is crucial for neuropeptide Y NPY  brain derived neurotrophic factor BDNF  and nerve growth factor receptor NGFR expressions  which are crucial for synaptic integrity  astroglial proliferation  and microglial prevalence. Impaired NPY induction decreased BDNF and synaptic density  which are rescuable with ectopic NPY. In induced pluripotent stem cell derived human neurons exposed to Aß42  ABCA7 /  suppresses NPY. Clinical data showed reduced NPY in AD correlated with elevated Braak stages  genetic variants in NPY associated with AD  and epigenetic changes in NPY  NGFR  and BDNF promoters linked to ABCA7 variants. Therefore  ABCA7 dependent NPY signaling via BDNF NGFR maintains synaptic integrity  implicating its impairment in increased AD risk through reduced brain resilience. Overall design: WT and abca7+/  zebrafish injected with Amyloid beta 42 peptide as described Bhatatrai et al. 2016 and cells were dissociated  sorted by FACS as described Cosacak et al. 2019. The single cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome GRChZ 11  v 105 was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data,,pubmed:39216475,,telencephalon  WT  PBS injected,GSM7819015,,source name:telencephalon|tissue:telencephalon|genotype:WT AB|treatment:PBS|geo loc name:missing|collection date:missing,telencephalon  WT  PBS injected,The demultiplexing  barcoded processing  gene counting and aggregation were made using the 10x genomics' cellranger software version 6.1.2 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/output/metrics The fastq files were aligned to zebrafish genome GRCz11 and ensemble transcripts from Ensembl Release 105 by using STAR. The BAM files were as input for Cell Ranger 10X genomics to generate processed data files that include gene names row names and cell names column names and counts. Further analysis done by using Seurat package in R. Assembly: GRCz11 Supplementary files format and content: filtered bc matrix outputs from Cell Ranger,telencephalon,WT AB and abca7 +/  knock out lines were injected with Amyloid beta 42 peptide  and PBS as control as descibed Bhatatrai et al. 2016.,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,Zebrafish were kept in the re circulating system on a 14/10 h light/dark cycle  pH7.5  at 28°C ±1°C in groups of 20 animals per 2.8L.,tissue:telencephalon|genotype:WT AB|treatment:PBS,GSM7819015,GSM7819015: telencephalon  WT  PBS injected; Danio rerio; RNA Seq,GSM7819015 r1,GSM7819015,1,Cells from zebrafish telencephalon were dissociated. Viability indicator dyes Sytox Blue Invitrogen  Cat No. S34857 and Dycle Ruby Invitrogen  Cat. No. V10309 were used to sort the cells by FACS. The library preparation was performed by 10X Genomics as per manufacture's protocol. 10X genomics,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP464334,,loader:fastq load.py,CP018_S9_L002_I1_001.fastq.gz CP018_S9_L002_I2_001.fastq.gz CP018_S9_L002_R1_001.fastq.gz CP018_S9_L002_R2_001.fastq.gz,fastq fastq fastq fastq,47434063566.0,213666953.0,GSM7819015 r2,0:10 1:10 2:101 3:101,A:12506355665;C:7237420299;G:7414157832;T:16002190524;N:600186,10,10,101,101,12506355665,7237420299,7414157832,16002190524,600186,SRX21975995,SRS19051831,SRA1725563,"Neurology &amp; TAUB Institute, Columbia University","Neurology & TAUB Institute, Columbia University",2,0.00327,0.8932,0.00092,0.30198,0.99831,0.73401,0.53488,0.50008,101,101,T,B,mate1 technical by mapping diff,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2023-10-03,Undetermined,Undetermined,Brain,Nervous System
28692,SRR26535343,SRX22238470,SRS19292731,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 2,GSM7866394,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 2,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866394,GSM7866394: EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 2; Danio rerio; RNA Seq,GSM7866394 r1,GSM7866394,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,CTRL_3.fastq.gz,fastq,7081948098.0,70118298.0,GSM7866394 r1,0:101,A:1894557984;C:1627945034;G:1550203852;T:2009224463;N:16765,101,,,,1894557984,1627945034,1550203852,2009224463,16765,SRX22238470,SRS19292731,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.94543,,0.14646,,0.68883,,0.49451,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28693,SRR26535344,SRX22238469,SRS19292730,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 1,GSM7866393,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 1,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866393,GSM7866393: EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 1; Danio rerio; RNA Seq,GSM7866393 r1,GSM7866393,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,CTRL_2.fastq.gz,fastq,7840572329.0,77629429.0,GSM7866393 r1,0:101,A:2099360211;C:1783653297;G:1717681461;T:2239858766;N:18594,101,,,,2099360211,1783653297,1717681461,2239858766,18594,SRX22238469,SRS19292730,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.93984,,0.1663,,0.69033,,0.484,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28694,SRR26535345,SRX22238468,SRS19292729,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,KRAS PI3KCAH1047R  GFP tumor negative control brain,GSM7866392,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,KRAS PI3KCAH1047R  GFP tumor negative control brain,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866392,GSM7866392: KRAS PI3KCAH1047R  GFP tumor negative control brain; Danio rerio; RNA Seq,GSM7866392 r1,GSM7866392,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,CTRL_1.fastq.gz,fastq,10164914720.0,100642720.0,GSM7866392 r1,0:101,A:2650336113;C:2354113843;G:2276252979;T:2884188285;N:23500,101,,,,2650336113,2354113843,2276252979,2884188285,23500,SRX22238468,SRS19292729,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.94271,,0.10831,,0.68527,,0.50207,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28695,SRR26535346,SRX22238467,SRS19292728,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 3,GSM7866391,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 3,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866391,GSM7866391: EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 3; Danio rerio; RNA Seq,GSM7866391 r1,GSM7866391,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,EPS_3.fastq.gz,fastq,9796714170.0,96997170.0,GSM7866391 r1,0:101,A:2627522717;C:2223655583;G:2134761894;T:2810751194;N:22782,101,,,,2627522717,2223655583,2134761894,2810751194,22782,SRX22238467,SRS19292728,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.92543,,0.1876,,0.67308,,0.4886,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28696,SRR26535347,SRX22238466,SRS19292727,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 2,GSM7866390,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 2,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866390,GSM7866390: EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 2; Danio rerio; RNA Seq,GSM7866390 r1,GSM7866390,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,EPS_2.fastq.gz,fastq,7839595861.0,77619761.0,GSM7866390 r1,0:101,A:2093456023;C:1792947580;G:1718784421;T:2234389453;N:18384,101,,,,2093456023,1792947580,1718784421,2234389453,18384,SRX22238466,SRS19292727,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.93807,,0.14978,,0.68854,,0.47786,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System