rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
14875,ERR12071836,ERX11454461,ERS16387703,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 1,SAMEA114399011,University of East Anglia,ENA FIRST PUBLIC:2023 10 06T08:31:44Z|ENA LAST UPDATE:2023 10 06T08:31:44Z|External Id:SAMEA114399011|INSDC center name:University of East Anglia|INSDC first public:2023 10 06T08:31:44Z|INSDC last update:2023 10 06T08:31:44Z|INSDC status:public|Submitter Id:115 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66715,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 10 06|ENA LAST UPDATE:2023 10 06,115-1_S69_L006_R1_001.fastq.gz 115-1_S69_L006_R2_001.fastq.gz,fastq fastq,12813280704.0,50846352.0,ena RUN TAB 21 09 2023 13:32:15:295 66716,0:126 1:126,A:2451295493;C:3891843753;G:4001866488;T:2448421880;N:19853090,126,126,,,2451295493,3891843753,4001866488,2448421880,19853090,ERX11454461,ERS16387703,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89907,0.8514,0.17845,0.173,0.80369,0.81085,0.83252,0.83449,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14876,ERR12071855,ERX11454480,ERS16387722,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 5,SAMEA114399030,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:300 66753,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-5_S40_L008_R1_001.fastq.gz 83-5_S40_L008_R2_001.fastq.gz,fastq fastq,13683493656.0,54299578.0,ena RUN TAB 21 09 2023 13:32:15:300 66754,0:126 1:126,A:2772843483;C:4073415925;G:4192640412;T:2641952920;N:2640916,126,126,,,2772843483,4073415925,4192640412,2641952920,2640916,ERX11454480,ERS16387722,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92452,0.92427,0.15846,0.16251,0.7777,0.77666,0.78362,0.77515,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14877,ERR12071854,ERX11454479,ERS16387721,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 4,SAMEA114399029,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66751,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-4_S8_L002_R1_001.fastq.gz 83-4_S8_L002_R2_001.fastq.gz,fastq fastq,10045714644.0,39863947.0,ena RUN TAB 21 09 2023 13:32:15:300 66752,0:126 1:126,A:2037388456;C:2963530056;G:3069663461;T:1972981871;N:2150800,126,126,,,2037388456,2963530056,3069663461,1972981871,2150800,ERX11454479,ERS16387721,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91872,0.91762,0.16049,0.16307,0.76516,0.76644,0.77023,0.77199,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14878,ERR12071853,ERX11454478,ERS16387720,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 3,SAMEA114399028,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66749,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-3_S12_L003_R1_001.fastq.gz 83-3_S12_L003_R2_001.fastq.gz,fastq fastq,17333567748.0,68783999.0,ena RUN TAB 21 09 2023 13:32:15:299 66750,0:126 1:126,A:3399006637;C:5245167754;G:5416988818;T:3269621086;N:2783453,126,126,,,3399006637,5245167754,5416988818,3269621086,2783453,ERX11454478,ERS16387720,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93044,0.93099,0.14459,0.1479,0.77287,0.774,0.78248,0.78348,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14879,ERR12071852,ERX11454477,ERS16387719,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 1,SAMEA114399027,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66747,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-1_S4_L001_R1_001.fastq.gz 83-1_S4_L001_R2_001.fastq.gz,fastq fastq,14977164132.0,59433191.0,ena RUN TAB 21 09 2023 13:32:15:299 66748,0:126 1:126,A:3115980365;C:4357431505;G:4531888641;T:2968758582;N:3105039,126,126,,,3115980365,4357431505,4531888641,2968758582,3105039,ERX11454477,ERS16387719,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89622,0.89549,0.15698,0.16074,0.76558,0.76779,0.70301,0.73129,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14880,ERR12071851,ERX11454476,ERS16387718,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 5,SAMEA114399026,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66745,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-5_S3_L001_R1_001.fastq.gz 82-5_S3_L001_R2_001.fastq.gz,fastq fastq,14575744764.0,57840257.0,ena RUN TAB 21 09 2023 13:32:15:299 66746,0:126 1:126,A:3079286238;C:4161280385;G:4315514217;T:3016549159;N:3114765,126,126,,,3079286238,4161280385,4315514217,3016549159,3114765,ERX11454476,ERS16387718,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.82553,0.82489,0.15941,0.16124,0.77337,0.77518,0.73773,0.75718,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14881,ERR12071850,ERX11454475,ERS16387717,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 3,SAMEA114399025,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66743,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-3_S11_L003_R1_001.fastq.gz 82-3_S11_L003_R2_001.fastq.gz,fastq fastq,15753924144.0,62515572.0,ena RUN TAB 21 09 2023 13:32:15:299 66744,0:126 1:126,A:3294606329;C:4538635184;G:4708756438;T:3209425450;N:2500743,126,126,,,3294606329,4538635184,4708756438,3209425450,2500743,ERX11454475,ERS16387717,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.8642,0.86388,0.1596,0.16298,0.77051,0.77297,0.7599,0.7352,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14882,ERR12071849,ERX11454474,ERS16387716,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 2,SAMEA114399024,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66741,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 22,82-2_S7_L002_R1_001.fastq.gz 82-2_S7_L002_R2_001.fastq.gz,fastq fastq,20680686684.0,82066217.0,ena RUN TAB 21 09 2023 13:32:15:298 66742,0:126 1:126,A:4358995918;C:5919331822;G:6101024757;T:4296885228;N:4448959,126,126,,,4358995918,5919331822,6101024757,4296885228,4448959,ERX11454474,ERS16387716,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.88722,0.88591,0.1721,0.1755,0.76207,0.76394,0.74661,0.7483,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14883,ERR12071848,ERX11454473,ERS16387715,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 1,SAMEA114399023,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66739,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-1_S39_L008_R1_001.fastq.gz 82-1_S39_L008_R2_001.fastq.gz,fastq fastq,19062862812.0,75646281.0,ena RUN TAB 21 09 2023 13:32:15:298 66740,0:126 1:126,A:4015412618;C:5485657253;G:5643544062;T:3914646370;N:3602509,126,126,,,4015412618,5485657253,5643544062,3914646370,3602509,ERX11454473,ERS16387715,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.82331,0.82289,0.14959,0.15262,0.77786,0.77368,0.75014,0.71846,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14884,ERR12071847,ERX11454472,ERS16387714,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 5,SAMEA114399022,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66737,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-5_S6_L002_R1_001.fastq.gz 81-5_S6_L002_R2_001.fastq.gz,fastq fastq,14105505960.0,55974230.0,ena RUN TAB 21 09 2023 13:32:15:298 66738,0:126 1:126,A:2867593807;C:4154280778;G:4295901883;T:2784713422;N:3016070,126,126,,,2867593807,4154280778,4295901883,2784713422,3016070,ERX11454472,ERS16387714,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89055,0.89059,0.15225,0.15462,0.77031,0.7709,0.77204,0.74565,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14885,ERR12071846,ERX11454471,ERS16387713,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 4,SAMEA114399021,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66735,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-4_S38_L008_R1_001.fastq.gz 81-4_S38_L008_R2_001.fastq.gz,fastq fastq,14056344540.0,55779145.0,ena RUN TAB 21 09 2023 13:32:15:298 66736,0:126 1:126,A:2941182079;C:4065026103;G:4178339299;T:2869101906;N:2695153,126,126,,,2941182079,4065026103,4178339299,2869101906,2695153,ERX11454471,ERS16387713,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.85089,0.84987,0.16263,0.16502,0.77837,0.77283,0.74931,0.72487,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14886,ERR12071845,ERX11454470,ERS16387712,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 2,SAMEA114399020,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66733,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-2_S10_L003_R2_001.fastq.gz 81-2_S10_L003_R1_001.fastq.gz,fastq fastq,12344567004.0,48986377.0,ena RUN TAB 21 09 2023 13:32:15:297 66734,0:126 1:126,A:2564870489;C:3577715066;G:3699995887;T:2500054422;N:1931140,126,126,,,2564870489,3577715066,3699995887,2500054422,1931140,ERX11454470,ERS16387712,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.81332,0.81278,0.14345,0.14595,0.77962,0.78011,0.77642,0.76714,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14887,ERR12071844,ERX11454469,ERS16387711,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 1,SAMEA114399019,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66731,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-1_S2_L001_R1_001.fastq.gz 81-1_S2_L001_R2_001.fastq.gz,fastq fastq,16863847056.0,66920028.0,ena RUN TAB 21 09 2023 13:32:15:297 66732,0:126 1:126,A:3484102096;C:4908704805;G:5083802657;T:3383595635;N:3641863,126,126,,,3484102096,4908704805,5083802657,3383595635,3641863,ERX11454469,ERS16387711,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90019,0.89959,0.16463,0.16683,0.76607,0.76792,0.76365,0.73132,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14888,ERR12071843,ERX11454468,ERS16387710,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 4,SAMEA114399018,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66729,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-4_S9_L003_R1_001.fastq.gz 80-4_S9_L003_R2_001.fastq.gz,fastq fastq,22230539604.0,88216427.0,ena RUN TAB 21 09 2023 13:32:15:297 66730,0:126 1:126,A:4399600032;C:6651638899;G:6855034908;T:4320719496;N:3546269,126,126,,,4399600032,6651638899,6855034908,4320719496,3546269,ERX11454468,ERS16387710,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84585,0.84448,0.14327,0.14586,0.79078,0.79251,0.79803,0.79459,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14889,ERR12071842,ERX11454467,ERS16387709,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 3,SAMEA114399017,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66727,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-3_S1_L001_R2_001.fastq.gz 80-3_S1_L001_R1_001.fastq.gz,fastq fastq,17944230024.0,71207262.0,ena RUN TAB 21 09 2023 13:32:15:296 66728,0:126 1:126,A:3713490828;C:5207225554;G:5378396527;T:3641393155;N:3723960,126,126,,,3713490828,5207225554,5378396527,3641393155,3723960,ERX11454467,ERS16387709,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84454,0.84386,0.14922,0.15129,0.77264,0.77339,0.75797,0.76206,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14890,ERR12071841,ERX11454466,ERS16387708,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 2,SAMEA114399016,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66725,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-2_S5_L002_R1_001.fastq.gz 80-2_S5_L002_R2_001.fastq.gz,fastq fastq,16641238320.0,66036660.0,ena RUN TAB 21 09 2023 13:32:15:296 66726,0:126 1:126,A:3508280857;C:4757668643;G:4898009962;T:3473768461;N:3510397,126,126,,,3508280857,4757668643,4898009962,3473768461,3510397,ERX11454466,ERS16387708,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.86106,0.86111,0.16002,0.16307,0.7653,0.76542,0.72623,0.70444,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14891,ERR12071840,ERX11454465,ERS16387707,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 1,SAMEA114399015,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66723,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-1_S37_L008_R1_001.fastq.gz 80-1_S37_L008_R2_001.fastq.gz,fastq fastq,15269215248.0,60592124.0,ena RUN TAB 21 09 2023 13:32:15:296 66724,0:126 1:126,A:3161128702;C:4439474080;G:4571245477;T:3094451760;N:2915229,126,126,,,3161128702,4439474080,4571245477,3094451760,2915229,ERX11454465,ERS16387707,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84008,0.83924,0.16203,0.16482,0.78486,0.78301,0.76847,0.76451,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14892,ERR12071839,ERX11454464,ERS16387706,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 5,SAMEA114399014,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66721,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-5_S28_L005_R2_001.fastq.gz 115-5_S28_L005_R1_001.fastq.gz,fastq fastq,13704997824.0,54384912.0,ena RUN TAB 21 09 2023 13:32:15:296 66722,0:126 1:126,A:2791048995;C:4040751875;G:4161655954;T:2708992373;N:2548627,126,126,,,2791048995,4040751875,4161655954,2708992373,2548627,ERX11454464,ERS16387706,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92152,0.922,0.16432,0.16746,0.77977,0.77723,0.7658,0.74319,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14893,ERR12071838,ERX11454463,ERS16387705,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 4,SAMEA114399013,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66719,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-4_S73_L007_R1_001.fastq.gz 115-4_S73_L007_R2_001.fastq.gz,fastq fastq,13555374840.0,53791170.0,ena RUN TAB 21 09 2023 13:32:15:295 66720,0:126 1:126,A:2613879041;C:4131073531;G:4251239682;T:2536011431;N:23171155,126,126,,,2613879041,4131073531,4251239682,2536011431,23171155,ERX11454463,ERS16387705,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93971,0.93992,0.15435,0.15669,0.78766,0.79076,0.76218,0.78676,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14894,ERR12071837,ERX11454462,ERS16387704,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 2,SAMEA114399012,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66717,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-2_S77_L008_R1_001.fastq.gz 115-2_S77_L008_R2_001.fastq.gz,fastq fastq,19309168872.0,76623686.0,ena RUN TAB 21 09 2023 13:32:15:295 66718,0:126 1:126,A:3659403129;C:5914313547;G:6102556630;T:3596021103;N:36874463,126,126,,,3659403129,5914313547,6102556630,3596021103,36874463,ERX11454462,ERS16387704,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90206,0.90085,0.164,0.16686,0.81477,0.81856,0.84674,0.83786,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14895,ERR12071835,ERX11454460,ERS16387702,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 5,SAMEA114399010,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66713,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-5_S68_L006_R1_001.fastq.gz 114-5_S68_L006_R2_001.fastq.gz,fastq fastq,14161249872.0,56195436.0,ena RUN TAB 21 09 2023 13:32:15:295 66714,0:126 1:126,A:2927386834;C:4098238005;G:4213138034;T:2900333935;N:22153064,126,126,,,2927386834,4098238005,4213138034,2900333935,22153064,ERX11454460,ERS16387702,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92035,0.91983,0.16785,0.17009,0.75864,0.76292,0.73081,0.75429,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14896,ERR12071834,ERX11454459,ERS16387701,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 4,SAMEA114399009,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66711,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-4_S76_L008_R1_001.fastq.gz 114-4_S76_L008_R2_001.fastq.gz,fastq fastq,12939456600.0,51347050.0,ena RUN TAB 21 09 2023 13:32:15:294 66712,0:126 1:126,A:2686900916;C:3725092053;G:3826228928;T:2676529407;N:24705296,126,126,,,2686900916,3725092053,3826228928,2676529407,24705296,ERX11454459,ERS16387701,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9171,0.91541,0.19718,0.20005,0.76759,0.77285,0.75547,0.75357,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14897,ERR12071833,ERX11454458,ERS16387700,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 3,SAMEA114399008,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66709,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-3_S72_L007_R1_001.fastq.gz 114-3_S72_L007_R2_001.fastq.gz,fastq fastq,15510255012.0,61548631.0,ena RUN TAB 21 09 2023 13:32:15:294 66710,0:126 1:126,A:3024458012;C:4641826332;G:4764527525;T:3052903565;N:26539578,126,126,,,3024458012,4641826332,4764527525,3052903565,26539578,ERX11454458,ERS16387700,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.85112,0.84868,0.17054,0.17432,0.79547,0.79851,0.73756,0.8091,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14898,ERR12071832,ERX11454457,ERS16387699,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 1,SAMEA114399007,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66707,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-1_S27_L005_R1_001.fastq.gz 114-1_S27_L005_R2_001.fastq.gz,fastq fastq,15544755576.0,61685538.0,ena RUN TAB 21 09 2023 13:32:15:294 66708,0:126 1:126,A:3077913021;C:4638218386;G:4760672592;T:3065112184;N:2839393,126,126,,,3077913021,4638218386,4760672592,3065112184,2839393,ERX11454457,ERS16387699,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90822,0.90777,0.1775,0.18112,0.79263,0.78808,0.82214,0.79489,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14899,ERR12071831,ERX11454456,ERS16387698,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 9,SAMEA114399006,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 9|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 9|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66705,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-9_S67_L006_R1_001.fastq.gz 113-9_S67_L006_R2_001.fastq.gz,fastq fastq,15414133644.0,61167197.0,ena RUN TAB 21 09 2023 13:32:15:293 66706,0:126 1:126,A:3185120611;C:4467167719;G:4606569127;T:3131094561;N:24181626,126,126,,,3185120611,4467167719,4606569127,3131094561,24181626,ERX11454456,ERS16387698,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94176,0.94197,0.17489,0.17743,0.76063,0.76295,0.75218,0.75546,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14900,ERR12071830,ERX11454455,ERS16387697,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 13,SAMEA114399005,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 13|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 13|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66703,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-13_S71_L007_R1_001.fastq.gz 113-13_S71_L007_R2_001.fastq.gz,fastq fastq,18125498160.0,71926580.0,ena RUN TAB 21 09 2023 13:32:15:293 66704,0:126 1:126,A:3681246224;C:5316613237;G:5486708206;T:3609735923;N:31194570,126,126,,,3681246224,5316613237,5486708206,3609735923,31194570,ERX11454455,ERS16387697,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93952,0.93814,0.1717,0.17395,0.76627,0.76976,0.76533,0.72702,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14901,ERR12071829,ERX11454454,ERS16387696,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 11,SAMEA114399004,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 11|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 11|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66701,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-11_S26_L005_R1_001.fastq.gz 113-11_S26_L005_R2_001.fastq.gz,fastq fastq,17918758872.0,71106186.0,ena RUN TAB 21 09 2023 13:32:15:293 66702,0:126 1:126,A:3460235614;C:5469489967;G:5622549540;T:3363214846;N:3268905,126,126,,,3460235614,5469489967,5622549540,3363214846,3268905,ERX11454454,ERS16387696,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94836,0.94773,0.16512,0.16708,0.79843,0.79251,0.79716,0.79211,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14902,ERR12071828,ERX11454453,ERS16387695,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 10,SAMEA114399003,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 10|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 10|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66699,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-10_S75_L008_R1_001.fastq.gz 113-10_S75_L008_R2_001.fastq.gz,fastq fastq,22137738588.0,87848169.0,ena RUN TAB 21 09 2023 13:32:15:293 66700,0:126 1:126,A:4240961737;C:6714114283;G:6924397580;T:4216314997;N:41949991,126,126,,,4240961737,6714114283,6924397580,4216314997,41949991,ERX11454453,ERS16387695,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94101,0.93949,0.15969,0.16256,0.782,0.78429,0.78877,0.78359,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14903,ERR12071827,ERX11454452,ERS16387694,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 8,SAMEA114399002,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 8|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 8|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66697,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-8_S74_L008_R1_001.fastq.gz 112-8_S74_L008_R2_001.fastq.gz,fastq fastq,15579825912.0,61824706.0,ena RUN TAB 21 09 2023 13:32:15:292 66698,0:126 1:126,A:3262384535;C:4470619467;G:4585527202;T:3231451715;N:29842993,126,126,,,3262384535,4470619467,4585527202,3231451715,29842993,ERX11454452,ERS16387694,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92603,0.92484,0.18249,0.18589,0.75917,0.76335,0.73196,0.72728,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14904,ERR12071826,ERX11454451,ERS16387693,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 7,SAMEA114399001,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 7|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 7|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66695,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-7_S66_L006_R1_001.fastq.gz 112-7_S66_L006_R2_001.fastq.gz,fastq fastq,20203529976.0,80172738.0,ena RUN TAB 21 09 2023 13:32:15:292 66696,0:126 1:126,A:4115948087;C:5902381830;G:6064166888;T:4089530757;N:31502414,126,126,,,4115948087,5902381830,6064166888,4089530757,31502414,ERX11454451,ERS16387693,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92896,0.92845,0.17083,0.17434,0.76146,0.76337,0.6945,0.74323,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14905,ERR12071825,ERX11454450,ERS16387692,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 5,SAMEA114399000,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66693,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-5_S70_L007_R1_001.fastq.gz 112-5_S70_L007_R2_001.fastq.gz,fastq fastq,14979433392.0,59442196.0,ena RUN TAB 21 09 2023 13:32:15:292 66694,0:126 1:126,A:2989976300;C:4427492967;G:4545641644;T:2990769657;N:25552824,126,126,,,2989976300,4427492967,4545641644,2990769657,25552824,ERX11454450,ERS16387692,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9139,0.91293,0.18796,0.1912,0.78443,0.78597,0.79005,0.77582,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14906,ERR12071824,ERX11454449,ERS16387691,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 4,SAMEA114398999,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66691,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-4_S25_L005_R1_001.fastq.gz 112-4_S25_L005_R2_001.fastq.gz,fastq fastq,14850028368.0,58928684.0,ena RUN TAB 21 09 2023 13:32:15:292 66692,0:126 1:126,A:3108605429;C:4289376091;G:4392727411;T:3056545411;N:2774026,126,126,,,3108605429,4289376091,4392727411,3056545411,2774026,ERX11454449,ERS16387691,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92451,0.92555,0.17853,0.18265,0.76501,0.76106,0.73074,0.72504,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14907,ERR12071823,ERX11454448,ERS16387690,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 4,SAMEA114398998,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66689,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-4_S8_L002_R1_001.fastq.gz 111-4_S8_L002_R2_001.fastq.gz,fastq fastq,17826911676.0,70741713.0,ena RUN TAB 21 09 2023 13:32:15:291 66690,0:126 1:126,A:3435687537;C:5460019012;G:5625529849;T:3303228205;N:2447073,126,126,,,3435687537,5460019012,5625529849,3303228205,2447073,ERX11454448,ERS16387690,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95917,0.95936,0.18841,0.18992,0.81367,0.81523,0.79429,0.81623,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14908,ERR12071822,ERX11454447,ERS16387689,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 3,SAMEA114398997,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66687,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-3_S16_L004_R1_001.fastq.gz 111-3_S16_L004_R2_001.fastq.gz,fastq fastq,16893915192.0,67039346.0,ena RUN TAB 21 09 2023 13:32:15:291 66688,0:126 1:126,A:3264486030;C:5167828698;G:5330646003;T:3128597235;N:2357226,126,126,,,3264486030,5167828698,5330646003,3128597235,2357226,ERX11454447,ERS16387689,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96382,0.96339,0.19876,0.20112,0.81893,0.82102,0.81987,0.79966,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14909,ERR12071821,ERX11454446,ERS16387688,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 2,SAMEA114398996,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66685,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-2_S12_L003_R1_001.fastq.gz 111-2_S12_L003_R2_001.fastq.gz,fastq fastq,19135485684.0,75934467.0,ena RUN TAB 21 09 2023 13:32:15:291 66686,0:126 1:126,A:3609533802;C:5946063742;G:6161038930;T:3416257009;N:2592201,126,126,,,3609533802,5946063742,6161038930,3416257009,2592201,ERX11454446,ERS16387688,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96183,0.96186,0.17579,0.17791,0.81972,0.82047,0.77379,0.82377,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14910,ERR12071820,ERX11454445,ERS16387687,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 1,SAMEA114398995,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66683,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-1_S4_L001_R1_001.fastq.gz 111-1_S4_L001_R2_001.fastq.gz,fastq fastq,21082514796.0,83660773.0,ena RUN TAB 21 09 2023 13:32:15:291 66684,0:126 1:126,A:4043020782;C:6480787651;G:6689150606;T:3865255761;N:4299996,126,126,,,4043020782,6480787651,6689150606,3865255761,4299996,ERX11454445,ERS16387687,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96289,0.963,0.18676,0.18828,0.81791,0.81988,0.78774,0.81813,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14911,ERR12071819,ERX11454444,ERS16387686,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 4,SAMEA114398994,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66681,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-4_S3_L001_R2_001.fastq.gz 110-4_S3_L001_R1_001.fastq.gz,fastq fastq,14687310204.0,58282977.0,ena RUN TAB 21 09 2023 13:32:15:290 66682,0:126 1:126,A:2926150199;C:4367282172;G:4510560380;T:2880182221;N:3135232,126,126,,,2926150199,4367282172,4510560380,2880182221,3135232,ERX11454444,ERS16387686,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93442,0.934,0.1706,0.17527,0.77611,0.7782,0.79075,0.77981,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14912,ERR12071818,ERX11454443,ERS16387685,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 3,SAMEA114398993,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66679,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-3_S11_L003_R2_001.fastq.gz 110-3_S11_L003_R1_001.fastq.gz,fastq fastq,13850712792.0,54963146.0,ena RUN TAB 21 09 2023 13:32:15:290 66680,0:126 1:126,A:2754372507;C:4139837776;G:4290528181;T:2664097645;N:1876683,126,126,,,2754372507,4139837776,4290528181,2664097645,1876683,ERX11454443,ERS16387685,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94187,0.94169,0.16695,0.17006,0.77824,0.78044,0.7861,0.7945,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14913,ERR12071817,ERX11454442,ERS16387684,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 2,SAMEA114398992,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66677,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-2_S15_L004_R2_001.fastq.gz 110-2_S15_L004_R1_001.fastq.gz,fastq fastq,15866607708.0,62962729.0,ena RUN TAB 21 09 2023 13:32:15:290 66678,0:126 1:126,A:3214891728;C:4688310800;G:4841314699;T:3119879854;N:2210627,126,126,,,3214891728,4688310800,4841314699,3119879854,2210627,ERX11454442,ERS16387684,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93647,0.93604,0.16699,0.17,0.76641,0.76909,0.76728,0.77117,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14914,ERR12071816,ERX11454441,ERS16387683,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 1,SAMEA114398991,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66675,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-1_S7_L002_R2_001.fastq.gz 110-1_S7_L002_R1_001.fastq.gz,fastq fastq,15365002212.0,60972231.0,ena RUN TAB 21 09 2023 13:32:15:290 66676,0:126 1:126,A:3114598527;C:4531528684;G:4684815069;T:3031977480;N:2082452,126,126,,,3114598527,4531528684,4684815069,3031977480,2082452,ERX11454441,ERS16387683,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93661,0.936,0.17032,0.17368,0.7699,0.77157,0.74201,0.77289,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14915,ERR12071815,ERX11454440,ERS16387682,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 4,SAMEA114398990,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66673,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-4_S14_L004_R1_001.fastq.gz 109-4_S14_L004_R2_001.fastq.gz,fastq fastq,17693786880.0,70213440.0,ena RUN TAB 21 09 2023 13:32:15:289 66674,,,,,,,,,,,,ERX11454440,ERS16387682,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93895,0.93626,0.13454,0.13567,0.80679,0.80906,0.83187,0.85713,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14916,ERR12071814,ERX11454439,ERS16387681,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 3,SAMEA114398989,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66671,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-3_S6_L002_R1_001.fastq.gz 109-3_S6_L002_R2_001.fastq.gz,fastq fastq,15326268300.0,60818525.0,ena RUN TAB 21 09 2023 13:32:15:289 66672,0:126 1:126,A:2963028716;C:4693248657;G:4853138006;T:2814772075;N:2080846,126,126,,,2963028716,4693248657,4853138006,2814772075,2080846,ERX11454439,ERS16387681,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95492,0.95516,0.18592,0.18766,0.816,0.81722,0.82037,0.77416,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14917,ERR12071813,ERX11454438,ERS16387680,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 2,SAMEA114398988,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66669,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-2_S9_L003_R1_001.fastq.gz 109-2_S9_L003_R2_001.fastq.gz,fastq fastq,13463300088.0,53425794.0,ena RUN TAB 21 09 2023 13:32:15:289 66670,0:126 1:126,A:2565791374;C:4152722401;G:4318203775;T:2424775489;N:1807049,126,126,,,2565791374,4152722401,4318203775,2424775489,1807049,ERX11454438,ERS16387680,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96145,0.9621,0.19448,0.19497,0.83382,0.83451,0.84292,0.79683,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14918,ERR12071812,ERX11454437,ERS16387679,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 1,SAMEA114398987,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66667,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-1_S1_L001_R2_001.fastq.gz 109-1_S1_L001_R1_001.fastq.gz,fastq fastq,16254330624.0,64501312.0,ena RUN TAB 21 09 2023 13:32:15:289 66668,0:126 1:126,A:3146898181;C:4962037311;G:5139473645;T:3002518241;N:3403246,126,126,,,3146898181,4962037311,5139473645,3002518241,3403246,ERX11454437,ERS16387679,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95901,0.9593,0.19067,0.19204,0.8174,0.81864,0.7987,0.8132,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14919,ERR12071811,ERX11454436,ERS16387678,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 4,SAMEA114398986,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66665,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-4_S10_L003_R1_001.fastq.gz 108-4_S10_L003_R2_001.fastq.gz,fastq fastq,16401717108.0,65086179.0,ena RUN TAB 21 09 2023 13:32:15:288 66666,0:126 1:126,A:3306091376;C:4834896468;G:4995683070;T:3262829888;N:2216306,126,126,,,3306091376,4834896468,4995683070,3262829888,2216306,ERX11454436,ERS16387678,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92612,0.92543,0.16352,0.16724,0.76607,0.76812,0.76693,0.75698,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14920,ERR12071810,ERX11454435,ERS16387677,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 3,SAMEA114398985,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66663,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-3_S2_L001_R2_001.fastq.gz 108-3_S2_L001_R1_001.fastq.gz,fastq fastq,14063114268.0,55806009.0,ena RUN TAB 21 09 2023 13:32:15:288 66664,0:126 1:126,A:2855140106;C:4126986255;G:4254731693;T:2823386830;N:2869384,126,126,,,2855140106,4126986255,4254731693,2823386830,2869384,ERX11454435,ERS16387677,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89956,0.89846,0.17394,0.17665,0.77695,0.77751,0.78333,0.77908,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14921,ERR12071809,ERX11454434,ERS16387676,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 2,SAMEA114398984,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66661,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-2_S13_L004_R1_001.fastq.gz 108-2_S13_L004_R2_001.fastq.gz,fastq fastq,17951370444.0,71235597.0,ena RUN TAB 21 09 2023 13:32:15:288 66662,0:126 1:126,A:3419406758;C:5496702371;G:5719743964;T:3312990750;N:2526601,126,126,,,3419406758,5496702371,5719743964,3312990750,2526601,ERX11454434,ERS16387676,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91308,0.91164,0.14161,0.14408,0.78725,0.78851,0.77166,0.81536,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14922,ERR12071808,ERX11454433,ERS16387675,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 1,SAMEA114398983,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66659,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-1_S5_L002_R1_001.fastq.gz 108-1_S5_L002_R2_001.fastq.gz,fastq fastq,17592295140.0,69810695.0,ena RUN TAB 21 09 2023 13:32:15:288 66660,0:126 1:126,A:3473054128;C:5265562453;G:5455395791;T:3395881406;N:2401362,126,126,,,3473054128,5265562453,5455395791,3395881406,2401362,ERX11454433,ERS16387675,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92632,0.92464,0.16285,0.16525,0.7781,0.77985,0.79539,0.79841,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14923,ERR12071807,ERX11454432,ERS16387674,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 4,SAMEA114398982,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66657,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-4_S20_L005_R1_001.fastq.gz 107-4_S20_L005_R2_001.fastq.gz,fastq fastq,12626647992.0,50105746.0,ena RUN TAB 21 09 2023 13:32:15:287 66658,0:126 1:126,A:2503116695;C:3776878144;G:3904732537;T:2438634661;N:3285955,126,126,,,2503116695,3776878144,3904732537,2438634661,3285955,ERX11454432,ERS16387674,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93409,0.93399,0.16467,0.16803,0.77567,0.77749,0.77962,0.76236,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14924,ERR12071806,ERX11454431,ERS16387673,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 3,SAMEA114398981,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66655,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-3_S28_L007_R1_001.fastq.gz 107-3_S28_L007_R2_001.fastq.gz,fastq fastq,19765610928.0,78434964.0,ena RUN TAB 21 09 2023 13:32:15:287 66656,0:126 1:126,A:3807913538;C:6041902918;G:6270499946;T:3639644996;N:5649530,126,126,,,3807913538,6041902918,6270499946,3639644996,5649530,ERX11454431,ERS16387673,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93479,0.93431,0.15154,0.15299,0.78462,0.78533,0.78429,0.79385,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14925,ERR12071805,ERX11454430,ERS16387672,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 2,SAMEA114398980,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66653,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-2_S32_L008_R1_001.fastq.gz 107-2_S32_L008_R2_001.fastq.gz,fastq fastq,20245786344.0,80340422.0,ena RUN TAB 21 09 2023 13:32:15:287 66654,0:126 1:126,A:3580212812;C:6483019868;G:6732117106;T:3443314781;N:7121777,126,126,,,3580212812,6483019868,6732117106,3443314781,7121777,ERX11454430,ERS16387672,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93526,0.93431,0.14042,0.14264,0.82887,0.83041,0.85552,0.85524,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14926,ERR12071804,ERX11454429,ERS16387671,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 1,SAMEA114398979,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66651,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-1_S24_L006_R2_001.fastq.gz 107-1_S24_L006_R1_001.fastq.gz,fastq fastq,15192276120.0,60286810.0,ena RUN TAB 21 09 2023 13:32:15:286 66652,0:126 1:126,A:2840014487;C:4709824279;G:4867364305;T:2771588673;N:3484376,126,126,,,2840014487,4709824279,4867364305,2771588673,3484376,ERX11454429,ERS16387671,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92039,0.91914,0.17007,0.17237,0.80939,0.80986,0.84885,0.847,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14927,ERR12071803,ERX11454428,ERS16387670,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 4,SAMEA114398978,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66649,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-4_S23_L006_R2_001.fastq.gz 106-4_S23_L006_R1_001.fastq.gz,fastq fastq,16985562300.0,67403025.0,ena RUN TAB 21 09 2023 13:32:15:286 66650,0:126 1:126,A:3322275260;C:5133904780;G:5334495158;T:3190965461;N:3921641,126,126,,,3322275260,5133904780,5334495158,3190965461,3921641,ERX11454428,ERS16387670,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93707,0.93661,0.15576,0.15861,0.77826,0.78025,0.77722,0.77979,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14928,ERR12071802,ERX11454427,ERS16387669,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 3,SAMEA114398977,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66647,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-3_S31_L008_R2_001.fastq.gz 106-3_S31_L008_R1_001.fastq.gz,fastq fastq,19459285524.0,77219387.0,ena RUN TAB 21 09 2023 13:32:15:286 66648,0:126 1:126,A:3823996295;C:5872737349;G:6122836793;T:3632881865;N:6833222,126,126,,,3823996295,5872737349,6122836793,3632881865,6833222,ERX11454427,ERS16387669,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90902,0.90816,0.1544,0.15713,0.78926,0.79176,0.76867,0.78913,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14929,ERR12071801,ERX11454426,ERS16387668,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 2,SAMEA114398976,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66645,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-2_S27_L007_R1_001.fastq.gz 106-2_S27_L007_R2_001.fastq.gz,fastq fastq,15694418124.0,62279437.0,ena RUN TAB 21 09 2023 13:32:15:286 66646,0:126 1:126,A:3002717019;C:4829058190;G:5037608362;T:2820533482;N:4501071,126,126,,,3002717019,4829058190,5037608362,2820533482,4501071,ERX11454426,ERS16387668,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93511,0.93528,0.14004,0.14226,0.7891,0.79068,0.77063,0.77891,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14930,ERR12071800,ERX11454425,ERS16387667,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 1,SAMEA114398975,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66643,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-1_S19_L005_R1_001.fastq.gz 106-1_S19_L005_R2_001.fastq.gz,fastq fastq,18647636868.0,73998559.0,ena RUN TAB 21 09 2023 13:32:15:285 66644,0:126 1:126,A:3692341453;C:5587227660;G:5803402834;T:3559657331;N:5007590,126,126,,,3692341453,5587227660,5803402834,3559657331,5007590,ERX11454425,ERS16387667,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92714,0.92688,0.16214,0.16642,0.77433,0.775,0.76217,0.75322,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14931,ERR12071799,ERX11454424,ERS16387666,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 4,SAMEA114398974,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66641,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-4_S26_L007_R2_001.fastq.gz 105-4_S26_L007_R1_001.fastq.gz,fastq fastq,15124522392.0,60017946.0,ena RUN TAB 21 09 2023 13:32:15:285 66642,0:126 1:126,A:2852895871;C:4689063320;G:4867825391;T:2710443233;N:4294577,126,126,,,2852895871,4689063320,4867825391,2710443233,4294577,ERX11454424,ERS16387666,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95529,0.95479,0.13148,0.13281,0.7793,0.78125,0.78337,0.78508,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14932,ERR12071798,ERX11454423,ERS16387665,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 3,SAMEA114398973,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66639,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-3_S18_L005_R1_001.fastq.gz 105-3_S18_L005_R2_001.fastq.gz,fastq fastq,17644367664.0,70017332.0,ena RUN TAB 21 09 2023 13:32:15:285 66640,0:126 1:126,A:3347478814;C:5457952160;G:5642901286;T:3191260656;N:4774748,126,126,,,3347478814,5457952160,5642901286,3191260656,4774748,ERX11454423,ERS16387665,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94866,0.94893,0.14303,0.14517,0.78658,0.78697,0.80916,0.81131,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14933,ERR12071797,ERX11454422,ERS16387664,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 2,SAMEA114398972,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66637,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-2_S30_L008_R1_001.fastq.gz 105-2_S30_L008_R2_001.fastq.gz,fastq fastq,15981084252.0,63417001.0,ena RUN TAB 21 09 2023 13:32:15:285 66638,0:126 1:126,A:3059876138;C:4898463877;G:5076925794;T:2940239108;N:5579335,126,126,,,3059876138,4898463877,5076925794,2940239108,5579335,ERX11454422,ERS16387664,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95109,0.95022,0.14624,0.14776,0.78066,0.78283,0.76738,0.79238,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14934,ERR12071796,ERX11454421,ERS16387663,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 1,SAMEA114398971,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66635,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-1_S22_L006_R1_001.fastq.gz 105-1_S22_L006_R2_001.fastq.gz,fastq fastq,16378597368.0,64994434.0,ena RUN TAB 21 09 2023 13:32:15:284 66636,0:126 1:126,A:3157768088;C:4992606167;G:5190605482;T:3033853059;N:3764572,126,126,,,3157768088,4992606167,5190605482,3033853059,3764572,ERX11454421,ERS16387663,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9472,0.94619,0.15768,0.15997,0.78646,0.78845,0.75864,0.79159,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14935,ERR12071795,ERX11454420,ERS16387662,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 4,SAMEA114398970,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66633,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-4_S29_L008_R2_001.fastq.gz 104-4_S29_L008_R1_001.fastq.gz,fastq fastq,17713234476.0,70290613.0,ena RUN TAB 21 09 2023 13:32:15:284 66634,0:126 1:126,A:3387663411;C:5419734351;G:5646953646;T:3252634316;N:6248752,126,126,,,3387663411,5419734351,5646953646,3252634316,6248752,ERX11454420,ERS16387662,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91957,0.91797,0.14938,0.15201,0.79149,0.79358,0.7508,0.80456,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14936,ERR12071794,ERX11454419,ERS16387661,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 3,SAMEA114398969,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66631,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-3_S21_L006_R1_001.fastq.gz 104-3_S21_L006_R2_001.fastq.gz,fastq fastq,20412904428.0,81003589.0,ena RUN TAB 21 09 2023 13:32:15:284 66632,0:126 1:126,A:3999139733;C:6180998229;G:6441889554;T:3786215014;N:4661898,126,126,,,3999139733,6180998229,6441889554,3786215014,4661898,ERX11454419,ERS16387661,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93627,0.93494,0.14845,0.14979,0.77796,0.77891,0.76434,0.77202,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14937,ERR12071793,ERX11454418,ERS16387660,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 2,SAMEA114398968,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66629,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-2_S25_L007_R1_001.fastq.gz 104-2_S25_L007_R2_001.fastq.gz,fastq fastq,15067973340.0,59793545.0,ena RUN TAB 21 09 2023 13:32:15:284 66630,0:126 1:126,A:2963040605;C:4540311477;G:4736958045;T:2823360579;N:4302634,126,126,,,2963040605,4540311477,4736958045,2823360579,4302634,ERX11454418,ERS16387660,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92968,0.92854,0.16053,0.16248,0.78224,0.78228,0.7365,0.76845,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14938,ERR12071792,ERX11454417,ERS16387659,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 1,SAMEA114398967,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66627,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-1_S17_L005_R1_001.fastq.gz 104-1_S17_L005_R2_001.fastq.gz,fastq fastq,19257494508.0,76418629.0,ena RUN TAB 21 09 2023 13:32:15:283 66628,0:126 1:126,A:3731156408;C:5859411051;G:6100994986;T:3560909583;N:5022480,126,126,,,3731156408,5859411051,6100994986,3560909583,5022480,ERX11454417,ERS16387659,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92633,0.92586,0.14768,0.14948,0.78277,0.7833,0.7452,0.76737,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14939,ERR12071791,ERX11454416,ERS16387658,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 4,SAMEA114398966,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66625,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-4_S32_L006_R1_001.fastq.gz 103-4_S32_L006_R2_001.fastq.gz,fastq fastq,13085486820.0,51926535.0,ena RUN TAB 21 09 2023 13:32:15:283 66626,0:126 1:126,A:2567103783;C:3969617611;G:4069486176;T:2476759371;N:2519879,126,126,,,2567103783,3969617611,4069486176,2476759371,2519879,ERX11454416,ERS16387658,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93075,0.93242,0.14151,0.14331,0.774,0.76822,0.75401,0.76454,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14940,ERR12071790,ERX11454415,ERS16387657,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 3,SAMEA114398965,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66623,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-3_S65_L007_R2_001.fastq.gz 103-3_S65_L007_R1_001.fastq.gz,fastq fastq,11192352696.0,44414098.0,ena RUN TAB 21 09 2023 13:32:15:283 66624,0:126 1:126,A:2123359510;C:3455439467;G:3570545494;T:2039990322;N:3017903,126,126,,,2123359510,3455439467,3570545494,2039990322,3017903,ERX11454415,ERS16387657,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89542,0.89591,0.1351,0.13661,0.78948,0.79003,0.77414,0.80393,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14941,ERR12071789,ERX11454414,ERS16387656,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 2,SAMEA114398964,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66621,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-2_S36_L007_R1_001.fastq.gz 103-2_S36_L007_R2_001.fastq.gz,fastq fastq,10568377512.0,41938006.0,ena RUN TAB 21 09 2023 13:32:15:283 66622,0:126 1:126,A:2109516452;C:3179785410;G:3272667938;T:2004453838;N:1953874,126,126,,,2109516452,3179785410,3272667938,2004453838,1953874,ERX11454414,ERS16387656,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92729,0.92817,0.15094,0.1528,0.7685,0.76516,0.74143,0.74277,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14942,ERR12071788,ERX11454413,ERS16387655,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 1,SAMEA114398963,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66619,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-1_S69_L008_R1_001.fastq.gz 103-1_S69_L008_R2_001.fastq.gz,fastq fastq,9417798180.0,37372215.0,ena RUN TAB 21 09 2023 13:32:15:282 66620,0:126 1:126,A:1807793384;C:2891759578;G:2983948236;T:1730405095;N:3891887,126,126,,,1807793384,2891759578,2983948236,1730405095,3891887,ERX11454413,ERS16387655,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.8784,0.87802,0.13342,0.13445,0.789,0.78946,0.79547,0.79885,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14943,ERR12071787,ERX11454412,ERS16387654,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 4,SAMEA114398962,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66617,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-4_S35_L007_R2_001.fastq.gz 102-4_S35_L007_R1_001.fastq.gz,fastq fastq,15605254728.0,61925614.0,ena RUN TAB 21 09 2023 13:32:15:282 66618,0:126 1:126,A:3158918177;C:4616749707;G:4726409583;T:3100328707;N:2848554,126,126,,,3158918177,4616749707,4726409583,3100328707,2848554,ERX11454412,ERS16387654,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91674,0.91732,0.1668,0.16991,0.77569,0.77082,0.76006,0.76056,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14944,ERR12071786,ERX11454411,ERS16387653,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 3,SAMEA114398961,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66615,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-3_S68_L008_R1_001.fastq.gz 102-3_S68_L008_R2_001.fastq.gz,fastq fastq,20642076252.0,81913001.0,ena RUN TAB 21 09 2023 13:32:15:282 66616,0:126 1:126,A:3670199430;C:6557337612;G:6776033266;T:3629933781;N:8572163,126,126,,,3670199430,6557337612,6776033266,3629933781,8572163,ERX11454411,ERS16387653,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89984,0.89794,0.14393,0.14603,0.82079,0.82233,0.83939,0.8347,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14945,ERR12071785,ERX11454410,ERS16387652,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 2,SAMEA114398960,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66613,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 22,102-2_S31_L006_R1_001.fastq.gz 102-2_S31_L006_R2_001.fastq.gz,fastq fastq,23877621936.0,94752468.0,ena RUN TAB 21 09 2023 13:32:15:282 66614,0:126 1:126,A:3953279642;C:7876070920;G:8128759951;T:3914909602;N:4601821,126,126,,,3953279642,7876070920,8128759951,3914909602,4601821,ERX11454410,ERS16387652,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92602,0.85538,0.13016,0.12286,0.84508,0.84936,0.81613,0.88529,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14946,ERR12071784,ERX11454409,ERS16387651,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 1,SAMEA114398959,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66611,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-1_S64_L007_R1_001.fastq.gz 102-1_S64_L007_R2_001.fastq.gz,fastq fastq,20588587236.0,81700743.0,ena RUN TAB 21 09 2023 13:32:15:281 66612,0:126 1:126,A:3005365881;C:7167599347;G:7450281822;T:2959758690;N:5581496,126,126,,,3005365881,7167599347,7450281822,2959758690,5581496,ERX11454409,ERS16387651,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90225,0.89979,0.08909,0.09003,0.87071,0.87148,0.90162,0.83246,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14947,ERR12071783,ERX11454408,ERS16387650,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 4,SAMEA114398958,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66609,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-4_S30_L006_R1_001.fastq.gz 101-4_S30_L006_R2_001.fastq.gz,fastq fastq,12524862168.0,49701834.0,ena RUN TAB 21 09 2023 13:32:15:281 66610,0:126 1:126,A:2576891018;C:3688938717;G:3819872503;T:2436717421;N:2442509,126,126,,,2576891018,3688938717,3819872503,2436717421,2442509,ERX11454408,ERS16387650,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92878,0.92997,0.15322,0.15505,0.76315,0.76063,0.69783,0.67569,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14948,ERR12071782,ERX11454407,ERS16387649,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 3,SAMEA114398957,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66607,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-3_S34_L007_R1_001.fastq.gz 101-3_S34_L007_R2_001.fastq.gz,fastq fastq,11973395700.0,47513475.0,ena RUN TAB 21 09 2023 13:32:15:281 66608,0:126 1:126,A:2371769443;C:3605917515;G:3700919970;T:2292577579;N:2211193,126,126,,,2371769443,3605917515,3700919970,2292577579,2211193,ERX11454407,ERS16387649,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92217,0.92315,0.14568,0.1469,0.7724,0.76917,0.7521,0.75171,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14949,ERR12071781,ERX11454406,ERS16387648,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 2,SAMEA114398956,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66605,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-2_S63_L007_R2_001.fastq.gz 101-2_S63_L007_R1_001.fastq.gz,fastq fastq,10806069456.0,42881228.0,ena RUN TAB 21 09 2023 13:32:15:280 66606,0:126 1:126,A:2073944191;C:3321784426;G:3405352767;T:2002070301;N:2917771,126,126,,,2073944191,3321784426,3405352767,2002070301,2917771,ERX11454406,ERS16387648,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9236,0.92427,0.14372,0.14539,0.78084,0.78141,0.74294,0.78886,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14950,ERR12071780,ERX11454405,ERS16387647,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 1,SAMEA114398955,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66603,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-1_S67_L008_R1_001.fastq.gz 101-1_S67_L008_R2_001.fastq.gz,fastq fastq,17155416348.0,68077049.0,ena RUN TAB 21 09 2023 13:32:15:280 66604,0:126 1:126,A:3066178934;C:5490109793;G:5728819123;T:2863162486;N:7146012,126,126,,,3066178934,5490109793,5728819123,2863162486,7146012,ERX11454405,ERS16387647,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94204,0.94154,0.12549,0.1266,0.80653,0.80726,0.83737,0.8394,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14951,ERR12071779,ERX11454404,ERS16387646,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 4,SAMEA114398954,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66601,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-4_S33_L007_R1_001.fastq.gz 100-4_S33_L007_R2_001.fastq.gz,fastq fastq,20883396240.0,82870620.0,ena RUN TAB 21 09 2023 13:32:15:280 66602,0:126 1:126,A:4151890640;C:6231825391;G:6398810174;T:4097014520;N:3855515,126,126,,,4151890640,6231825391,6398810174,4097014520,3855515,ERX11454404,ERS16387646,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91603,0.9155,0.17576,0.1788,0.7781,0.7753,0.76044,0.78007,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14952,ERR12071778,ERX11454403,ERS16387645,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 3,SAMEA114398953,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66599,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-3_S29_L006_R1_001.fastq.gz 100-3_S29_L006_R2_001.fastq.gz,fastq fastq,17203126752.0,68266376.0,ena RUN TAB 21 09 2023 13:32:15:280 66600,0:126 1:126,A:3380289058;C:5172410817;G:5314572798;T:3332505592;N:3348487,126,126,,,3380289058,5172410817,5314572798,3332505592,3348487,ERX11454403,ERS16387645,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90457,0.90462,0.18582,0.18981,0.79947,0.79435,0.81648,0.81903,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14953,ERR12071777,ERX11454402,ERS16387644,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 2,SAMEA114398952,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:279 66597,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-2_S66_L008_R2_001.fastq.gz 100-2_S66_L008_R1_001.fastq.gz,fastq fastq,12181472100.0,48339175.0,ena RUN TAB 21 09 2023 13:32:15:279 66598,0:126 1:126,A:2169455038;C:3847243791;G:3951410714;T:2208297402;N:5065155,126,126,,,2169455038,3847243791,3951410714,2208297402,5065155,ERX11454402,ERS16387644,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.87873,0.8759,0.15894,0.16003,0.82446,0.82475,0.8538,0.82928,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
14954,ERR12071776,ERX11454401,ERS16387643,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels  ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi  and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi  and piRNAs  with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching  and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 1,SAMEA114398951,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:279 66595,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-1_S62_L007_R1_001.fastq.gz 100-1_S62_L007_R2_001.fastq.gz,fastq fastq,12598877592.0,49995546.0,ena RUN TAB 21 09 2023 13:32:15:279 66596,0:126 1:126,A:2443108338;C:3813175879;G:3922667630;T:2416483718;N:3442027,126,126,,,2443108338,3813175879,3922667630,2416483718,3442027,ERX11454401,ERS16387643,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9126,0.91287,0.18702,0.1895,0.79431,0.79373,0.78056,0.81778,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise
15504,ERR757154,ERX701784,ERS659467,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Normalization across length,SAMEA3251321,UNIVERSITY OF AMSTERDAM,ENA first public:2015 02 12|ENA last update:2015 02 12|External Id:SAMEA3251321|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2015 02 12T17:06:49Z|INSDC last update:2015 02 12T09:52:20Z|INSDC status:public|Submitter Id:Female X|common name:zebrafish|dev stage:Adult|sample name:Female X|sex:female,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 12 02 2015 09:52:08:968 2,RID0035,1,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2015 02 12|ENA LAST UPDATE:2018 11 16,RID0035_003.fastq.gz,fastq,275591336.0,7611054.0,ena RUN UNIVERSITY OF AMSTERDAM 12 02 2015 09:52:08:968 2,0:36.21,A:72212971;C:63331647;G:68054270;T:71992448;N:0,36,,,,72212971,63331647,68054270,71992448,0,ERX701784,ERS659467,ERA410345,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.76262,,0.4273,,0.8101,,0.56676,,8,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2015-02-12,Adult,Adult,Undetermined,Undetermined
15505,ERR757153,ERX701783,ERS659466,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Normalization across length,SAMEA3251320,UNIVERSITY OF AMSTERDAM,ENA first public:2015 02 12|ENA last update:2015 02 12|External Id:SAMEA3251320|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2015 02 12T17:06:49Z|INSDC last update:2015 02 12T09:52:20Z|INSDC status:public|Submitter Id:Female 002|common name:zebrafish|dev stage:Adult|sample name:Female 002|sex:female,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 12 02 2015 09:52:08:968 1,RID0035,1,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2015 02 12|ENA LAST UPDATE:2018 11 16,RID0035_006.fastq.gz,fastq,193706106.0,6343893.0,ena RUN UNIVERSITY OF AMSTERDAM 12 02 2015 09:52:08:968 1,0:30.53,A:52756888;C:42895374;G:46979869;T:51073975;N:0,30,,,,52756888,42895374,46979869,51073975,0,ERX701783,ERS659466,ERA410345,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.74991,,0.41319,,0.83002,,0.55823,,8,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2015-02-12,Adult,Adult,Undetermined,Undetermined
15506,ERR647638,ERX604074,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 7,RID0015,Fold Change,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_007.fastq.gz,fastq,181485343.0,7581675.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 7,0:23.94,A:49408107;C:35660562;G:44520624;T:51896050;N:0,23,,,,49408107,35660562,44520624,51896050,0,ERX604074,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.75831,,0.16017,,0.90723,,0.48684,,13,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15507,ERR647632,ERX604068,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 1,RID0015,Fold Change,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_001.fastq.gz,fastq,158714692.0,5407747.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 1,0:29.35,A:42098161;C:32991434;G:38596553;T:45028544;N:0,29,,,,42098161,32991434,38596553,45028544,0,ERX604068,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.75901,,0.20293,,0.89505,,0.48215,,24,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15508,ERR647636,ERX604072,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 5,RID0015,Fold Change,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_005.fastq.gz,fastq,124167412.0,4838491.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 5,0:25.66,A:33439510;C:24728035;G:30897675;T:35102192;N:0,25,,,,33439510,24728035,30897675,35102192,0,ERX604072,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.76027,,0.17783,,0.89692,,0.48421,,30,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15509,ERR647635,ERX604071,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 4,RID0015,Fold Change,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_004.fastq.gz,fastq,124786630.0,4701452.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 4,0:26.54,A:33803418;C:24800330;G:30482560;T:35700322;N:0,26,,,,33803418,24800330,30482560,35700322,0,ERX604071,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.74872,,0.18448,,0.89789,,0.50377,,12,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15510,ERR647634,ERX604070,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 3,RID0015,Fold Change,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_003.fastq.gz,fastq,110526752.0,4078127.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 3,0:27.10,A:29453167;C:22981184;G:27444083;T:30648318;N:0,27,,,,29453167,22981184,27444083,30648318,0,ERX604070,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.73961,,0.1804,,0.89686,,0.50069,,22,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15511,ERR647633,ERX604069,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 2,RID0015,Fold Change,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_002.fastq.gz,fastq,114721515.0,4468497.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 2,0:25.67,A:31387085;C:22123581;G:28270438;T:32940411;N:0,25,,,,31387085,22123581,28270438,32940411,0,ERX604069,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.75767,,0.17199,,0.90118,,0.49949,,22,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15512,ERR647645,ERX604081,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 14,RID0015,Reproducibility,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_014.fastq.gz,fastq,127742942.0,5487454.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 14,0:23.28,A:35700746;C:25577569;G:31187339;T:35277288;N:0,23,,,,35700746,25577569,31187339,35277288,0,ERX604081,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.72451,,0.1507,,0.91078,,0.47283,,30,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15513,ERR647644,ERX604080,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 13,RID0015,Reproducibility,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_013.fastq.gz,fastq,175319872.0,7193103.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 13,0:24.37,A:48008107;C:34657005;G:43047489;T:49607271;N:0,24,,,,48008107,34657005,43047489,49607271,0,ERX604080,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.75539,,0.16288,,0.90388,,0.47538,,22,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15514,ERR647643,ERX604079,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 12,RID0015,Reproducibility,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_012.fastq.gz,fastq,177443752.0,7567733.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 12,0:23.45,A:48143286;C:36300870;G:44060167;T:48939429;N:0,23,,,,48143286,36300870,44060167,48939429,0,ERX604079,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.74839,,0.15018,,0.90782,,0.49076,,27,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15515,ERR647642,ERX604078,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 11,RID0015,Reproducibility,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_011.fastq.gz,fastq,163389373.0,6320373.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 11,0:25.85,A:44540588;C:32608476;G:40066295;T:46174014;N:0,25,,,,44540588,32608476,40066295,46174014,0,ERX604078,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.75948,,0.17375,,0.90252,,0.47536,,33,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15516,ERR647641,ERX604077,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 10,RID0015,Reproducibility,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_010.fastq.gz,fastq,128059692.0,5701206.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 10,0:22.46,A:35418188;C:24802528;G:31593786;T:36245190;N:0,22,,,,35418188,24802528,31593786,36245190,0,ERX604077,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.72586,,0.1331,,0.92514,,0.4216,,22,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15517,ERR647637,ERX604073,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 6,RID0015,Fold Change,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_006.fastq.gz,fastq,165327404.0,6835740.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 6,0:24.19,A:45252916;C:32442683;G:40306020;T:47325785;N:0,24,,,,45252916,32442683,40306020,47325785,0,ERX604073,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.74944,,0.16,,0.91068,,0.47704,,21,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15518,ERR647640,ERX604076,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 9,RID0015,Reproducibility,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_009.fastq.gz,fastq,134426587.0,5846482.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 9,0:22.99,A:36803811;C:26283055;G:33442229;T:37897492;N:0,22,,,,36803811,26283055,33442229,37897492,0,ERX604076,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.74219,,0.13958,,0.91837,,0.47819,,22,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15519,ERR647639,ERX604075,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 8,RID0015,Fold Change,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0015_008.fastq.gz,fastq,164369360.0,6762174.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 8,0:24.31,A:44826982;C:33030972;G:40615427;T:45895979;N:0,24,,,,44826982,33030972,40615427,45895979,0,ERX604075,ERS557914,ERA363852,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.75513,,0.16254,,0.90534,,0.50535,,22,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15520,ERR647623,ERX604059,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 8,RID0017,EtOH conc. experiment,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0017_008.fastq.gz,fastq,198146766.0,7373392.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 8,0:26.87,A:50518864;C:42346985;G:50683068;T:54597849;N:0,26,,,,50518864,42346985,50683068,54597849,0,ERX604059,ERS557914,ERA363851,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.73719,,0.16567,,0.89869,,0.49695,,76,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15521,ERR647622,ERX604058,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 7,RID0017,EtOH conc. experiment,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0017_007.fastq.gz,fastq,240260456.0,9194064.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 7,0:26.13,A:62434032;C:50861577;G:60916218;T:66048629;N:0,26,,,,62434032,50861577,60916218,66048629,0,ERX604058,ERS557914,ERA363851,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.73042,,0.17017,,0.89509,,0.51784,,44,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15522,ERR647620,ERX604056,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 5,RID0017,EtOH conc. experiment,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0017_005.fastq.gz,fastq,140470735.0,4903805.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 5,0:28.65,A:35312706;C:29191443;G:36788541;T:39178045;N:0,28,,,,35312706,29191443,36788541,39178045,0,ERX604056,ERS557914,ERA363851,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.76081,,0.16609,,0.90216,,0.48155,,72,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined
15523,ERR647617,ERX604053,ERS557914,ERP007147,PRJEB7420,Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq,ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622,Other,There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First  sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second  given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example  the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly  sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.,,,,Male 001,SAMEA2796299,UNIVERSITY OF AMSTERDAM,Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL,,,,,,,,,Ion Torrent Proton sequencing,ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 2,RID0017,EtOH conc. experiment,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP007147,Ion Torrent Proton sequencing,ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16,RID0017_002.fastq.gz,fastq,113953599.0,4875815.0,ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 2,0:23.37,A:29576305;C:23096908;G:29656780;T:31623606;N:0,23,,,,29576305,23096908,29656780,31623606,0,ERX604053,ERS557914,ERA363851,UNIVERSITY OF AMSTERDAM,UNIVERSITY OF AMSTERDAM,1,0.70891,,0.13528,,0.91208,,0.4622,,24,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2014-10-01,Adult,Adult,Undetermined,Undetermined