rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 31715,SRR28508805,SRX24109965,SRS20897113,SRP498846,PRJNA1094457,Danio rerio Transcriptome or Gene expression,PRJNA1094457,Other,Atrial cardiomyopathy which often comes with underlying genetic defects has been recognized as a possible substrate of atrial fibrillation. MYH6 encodes a myosin heavy chain a MHC predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here we employed CRISPR/Cas9 to generate myh6 knockout zebrafish assessing cardiac function through diverse methodologies including video analysis ECG echocardiography transmission electron microscopy and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.,,,,myh6+/ 3,Sample 6,,strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:myh6+/ |BioSampleModel:Model organism or animal,,,,,,,,,myh6+/ 3,Sample 6,Sample 6,Sequencing data were analyzed by R studio Version 2023.03.0+386. Differentially expressed genes DEGs between the experimental myh6+/ and control wildtype groups were identified using the DESeq2 package.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP498846,,,7_230906N_S64_L004_R1_001.fastq.gz 7_230906N_S64_L004_R2_001.fastq.gz,fastq fastq,5096818364.0,16876882.0,7 230906N S64 L004 R1 001.fastq.gz,0:151 1:151,A:1417476599;C:1123713516;G:1180970526;T:1374564888;N:92835,151,151,,,1417476599,1123713516,1180970526,1374564888,92835,SRX24109965,SRS20897113,SRA1836610,Nanjing medical university|Cardiology,Nanjing medical university,2,0.92843,0.93025,0.09825,0.0937,0.7601,0.76081,0.46245,0.45548,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2024-03-31,Adult,Adult,Heart,Cardiovascular System 31716,SRR28508806,SRX24109964,SRS20897112,SRP498846,PRJNA1094457,Danio rerio Transcriptome or Gene expression,PRJNA1094457,Other,Atrial cardiomyopathy which often comes with underlying genetic defects has been recognized as a possible substrate of atrial fibrillation. MYH6 encodes a myosin heavy chain a MHC predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here we employed CRISPR/Cas9 to generate myh6 knockout zebrafish assessing cardiac function through diverse methodologies including video analysis ECG echocardiography transmission electron microscopy and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.,,,,myh6+/ 2,Sample 5,,strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:myh6+/ |BioSampleModel:Model organism or animal,,,,,,,,,myh6+/ 2,Sample 5,Sample 5,Sequencing data were analyzed by R studio Version 2023.03.0+386. Differentially expressed genes DEGs between the experimental myh6+/ and control wildtype groups were identified using the DESeq2 package.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP498846,,,5_230906N_S62_L004_R2_001.fastq.gz 5_230906N_S62_L004_R1_001.fastq.gz,fastq fastq,6194790402.0,20512551.0,5 230906N S62 L004 R1 001.fastq.gz,0:151 1:151,A:1714858851;C:1363381540;G:1448185824;T:1668247206;N:116981,151,151,,,1714858851,1363381540,1448185824,1668247206,116981,SRX24109964,SRS20897112,SRA1836610,Nanjing medical university|Cardiology,Nanjing medical university,2,0.93019,0.93196,0.09367,0.08848,0.75444,0.75741,0.48694,0.48749,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2024-03-31,Adult,Adult,Heart,Cardiovascular System 31717,SRR28508807,SRX24109963,SRS20897111,SRP498846,PRJNA1094457,Danio rerio Transcriptome or Gene expression,PRJNA1094457,Other,Atrial cardiomyopathy which often comes with underlying genetic defects has been recognized as a possible substrate of atrial fibrillation. MYH6 encodes a myosin heavy chain a MHC predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here we employed CRISPR/Cas9 to generate myh6 knockout zebrafish assessing cardiac function through diverse methodologies including video analysis ECG echocardiography transmission electron microscopy and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.,,,,myh6+/ 1,Sample 4,,strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:myh6+/ |BioSampleModel:Model organism or animal,,,,,,,,,myh6+/ 1,Sample 4,Sample 4,Sequencing data were analyzed by R studio Version 2023.03.0+386. Differentially expressed genes DEGs between the experimental myh6+/ and control wildtype groups were identified using the DESeq2 package.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP498846,,,1_230906N_S59_L004_R2_001.fastq.gz 1_230906N_S59_L004_R1_001.fastq.gz,fastq fastq,5707073690.0,18897595.0,1 230906N S59 L004 R1 001.fastq.gz,0:151 1:151,A:1590227722;C:1248119772;G:1327453455;T:1541167866;N:104875,151,151,,,1590227722,1248119772,1327453455,1541167866,104875,SRX24109963,SRS20897111,SRA1836610,Nanjing medical university|Cardiology,Nanjing medical university,2,0.92942,0.93069,0.10355,0.09914,0.75745,0.76041,0.48155,0.48754,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2024-03-31,Adult,Adult,Heart,Cardiovascular System 31718,SRR28508808,SRX24109962,SRS20897110,SRP498846,PRJNA1094457,Danio rerio Transcriptome or Gene expression,PRJNA1094457,Other,Atrial cardiomyopathy which often comes with underlying genetic defects has been recognized as a possible substrate of atrial fibrillation. MYH6 encodes a myosin heavy chain a MHC predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here we employed CRISPR/Cas9 to generate myh6 knockout zebrafish assessing cardiac function through diverse methodologies including video analysis ECG echocardiography transmission electron microscopy and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.,,,,WT 3,Sample 3,,strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:wild type|BioSampleModel:Model organism or animal,,,,,,,,,WT 3,Sample 3,Sample 3,Sequencing data were analyzed by R studio Version 2023.03.0+386. Differentially expressed genes DEGs between the experimental myh6+/ and control wildtype groups were identified using the DESeq2 package.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP498846,,,6_230906N_S63_L004_R1_001.fastq.gz 6_230906N_S63_L004_R2_001.fastq.gz,fastq fastq,6134248160.0,20312080.0,6 230906N S63 L004 R1 001.fastq.gz,0:151 1:151,A:1703789586;C:1348484209;G:1426369972;T:1655488984;N:115409,151,151,,,1703789586,1348484209,1426369972,1655488984,115409,SRX24109962,SRS20897110,SRA1836610,Nanjing medical university|Cardiology,Nanjing medical university,2,0.92941,0.93008,0.087,0.08333,0.76946,0.77033,0.47664,0.48975,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2024-03-31,Adult,Adult,Heart,Cardiovascular System 31719,SRR28508809,SRX24109961,SRS20897109,SRP498846,PRJNA1094457,Danio rerio Transcriptome or Gene expression,PRJNA1094457,Other,Atrial cardiomyopathy which often comes with underlying genetic defects has been recognized as a possible substrate of atrial fibrillation. MYH6 encodes a myosin heavy chain a MHC predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here we employed CRISPR/Cas9 to generate myh6 knockout zebrafish assessing cardiac function through diverse methodologies including video analysis ECG echocardiography transmission electron microscopy and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.,,,,WT 2,Sample 2,,strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:wild type|BioSampleModel:Model organism or animal,,,,,,,,,WT 2,Sample 2,Sample 2,Sequencing data were analyzed by R studio Version 2023.03.0+386. Differentially expressed genes DEGs between the experimental myh6+/ and control wildtype groups were identified using the DESeq2 package.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP498846,,,4_230906N_S61_L004_R1_001.fastq.gz 4_230906N_S61_L004_R2_001.fastq.gz,fastq fastq,6573338342.0,21766021.0,4 230906N S61 L004 R1 001.fastq.gz,0:151 1:151,A:1838220811;C:1442852455;G:1512987676;T:1779153110;N:124290,151,151,,,1838220811,1442852455,1512987676,1779153110,124290,SRX24109961,SRS20897109,SRA1836610,Nanjing medical university|Cardiology,Nanjing medical university,2,0.9373,0.93754,0.08495,0.07972,0.7766,0.77897,0.48726,0.50532,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2024-03-31,Adult,Adult,Heart,Cardiovascular System 31720,SRR28508810,SRX24109960,SRS20897108,SRP498846,PRJNA1094457,Danio rerio Transcriptome or Gene expression,PRJNA1094457,Other,Atrial cardiomyopathy which often comes with underlying genetic defects has been recognized as a possible substrate of atrial fibrillation. MYH6 encodes a myosin heavy chain a MHC predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here we employed CRISPR/Cas9 to generate myh6 knockout zebrafish assessing cardiac function through diverse methodologies including video analysis ECG echocardiography transmission electron microscopy and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.,,,,WT 1,Sample 1,,strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:wild type|BioSampleModel:Model organism or animal,,,,,,,,,WT 1,Sample 1,Sample 1,Sequencing data were analyzed by R studio Version 2023.03.0+386. Differentially expressed genes DEGs between the experimental myh6+/ and control wildtype groups were identified using the DESeq2 package.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP498846,,,3_230906N_S1_L004_R2_001.fastq.gz 3_230906N_S1_L004_R1_001.fastq.gz,fastq fastq,6962707546.0,23055323.0,3 230906N S1 L004 R1 001.fastq.gz,0:151 1:151,A:1938531865;C:1527591368;G:1608823948;T:1887630666;N:129699,151,151,,,1938531865,1527591368,1608823948,1887630666,129699,SRX24109960,SRS20897108,SRA1836610,Nanjing medical university|Cardiology,Nanjing medical university,2,0.84836,0.93866,0.08283,0.08602,0.7696,0.764,0.48014,0.48678,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2024-03-31,Adult,Adult,Heart,Cardiovascular System 52916,SRR9596460,SRX6362169,SRS5024083,SRP211964,PRJNA551130,Induction of adult cardiomyocyte renewal by Klf1,PRJNA551130,Other,In highly regenerative animals cardiac regeneration occurs innately through cardiomyocyte dedifferentiation and proliferation although the regenerative mechanisms remain unclear. This project contains raw sequencing data sets that were used to identify that klf1 a Kruppel like transcription factor essential for red blood cell development is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts,,,klf1 over expressing,,klf1,,strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:klf1 overexpressing|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish cardiac tissue,OE1 RNASEQ,OE1 RNASEQ,Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. RNA libraries were prepared 500ng input using the Kapa stranded RNA Seq kit using the oligo dT module to enrich for mRNA transcripts. Libraries were pooled to equimolar concentrations and sequenced on an Illumina HiSeq2500 paired end reads using v4 high output chemistry.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP211964,,,CCB6CANXX_5_180204_CFL--000_Other_ATCACG_R_180116_DAVHUM_LIB2500_M003_R1.fastq.gz CCB6CANXX_5_180204_CFL--000_Other_ATCACG_R_180116_DAVHUM_LIB2500_M003_R2.fastq.gz,fastq fastq,6046387500.0,24185550.0,CCB6CANXX 5 180204 CFL 000 Other ATCACG R 180116 DAVHUM LIB2500 M003 R1.fastq.gz,0:125 1:125,A:1668794703;C:1351121805;G:1358418631;T:1666534929;N:1517432,125,125,,,1668794703,1351121805,1358418631,1666534929,1517432,SRX6362169,SRS5024083,SRA913571,Victor Chang Cardiac Research Institute|Genome core,Victor Chang Cardiac Research Institute,2,0.93849,0.94275,0.11483,0.11515,0.73979,0.74251,0.49919,0.49697,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Australia,2019-06-26,Adult,Adult,Heart,Cardiovascular System 52917,SRR9596461,SRX6362168,SRS5024083,SRP211964,PRJNA551130,Induction of adult cardiomyocyte renewal by Klf1,PRJNA551130,Other,In highly regenerative animals cardiac regeneration occurs innately through cardiomyocyte dedifferentiation and proliferation although the regenerative mechanisms remain unclear. This project contains raw sequencing data sets that were used to identify that klf1 a Kruppel like transcription factor essential for red blood cell development is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts,,,klf1 over expressing,,klf1,,strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:klf1 overexpressing|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish cardiac tissue,OE2 RNASEQ,OE2 RNASEQ,Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. RNA libraries were prepared 500ng input using the Kapa stranded RNA Seq kit using the oligo dT module to enrich for mRNA transcripts. Libraries were pooled to equimolar concentrations and sequenced on an Illumina HiSeq2500 paired end reads using v4 high output chemistry.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP211964,,,CCB6CANXX_5_180204_CFL--002_Other_TTAGGC_R_180116_DAVHUM_LIB2500_M003_R1.fastq.gz CCB6CANXX_5_180204_CFL--002_Other_TTAGGC_R_180116_DAVHUM_LIB2500_M003_R2.fastq.gz,fastq fastq,6511647250.0,26046589.0,CCB6CANXX 5 180204 CFL 002 Other TTAGGC R 180116 DAVHUM LIB2500 M003 R1.fastq.gz,0:125 1:125,A:1807264422;C:1439461808;G:1458452089;T:1804785270;N:1683661,125,125,,,1807264422,1439461808,1458452089,1804785270,1683661,SRX6362168,SRS5024083,SRA913571,Victor Chang Cardiac Research Institute|Genome core,Victor Chang Cardiac Research Institute,2,0.93772,0.94197,0.11227,0.11206,0.73387,0.73689,0.55595,0.55246,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Australia,2019-06-26,Adult,Adult,Heart,Cardiovascular System 52918,SRR9596462,SRX6362167,SRS5024083,SRP211964,PRJNA551130,Induction of adult cardiomyocyte renewal by Klf1,PRJNA551130,Other,In highly regenerative animals cardiac regeneration occurs innately through cardiomyocyte dedifferentiation and proliferation although the regenerative mechanisms remain unclear. This project contains raw sequencing data sets that were used to identify that klf1 a Kruppel like transcription factor essential for red blood cell development is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts,,,klf1 over expressing,,klf1,,strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:klf1 overexpressing|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish cardiac tissue,OE3 RNASEQ,OE3 RNASEQ,Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. RNA libraries were prepared 500ng input using the Kapa stranded RNA Seq kit using the oligo dT module to enrich for mRNA transcripts. Libraries were pooled to equimolar concentrations and sequenced on an Illumina HiSeq2500 paired end reads using v4 high output chemistry.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP211964,,,CCB6CANXX_5_180204_CFL--006_Other_ACTTGA_R_180116_DAVHUM_LIB2500_M003_R1.fastq.gz CCB6CANXX_5_180204_CFL--006_Other_ACTTGA_R_180116_DAVHUM_LIB2500_M003_R2.fastq.gz,fastq fastq,6173169750.0,24692679.0,CCB6CANXX 5 180204 CFL 006 Other ACTTGA R 180116 DAVHUM LIB2500 M003 R1.fastq.gz,0:125 1:125,A:1706550006;C:1374108830;G:1394428093;T:1696508705;N:1574116,125,125,,,1706550006,1374108830,1394428093,1696508705,1574116,SRX6362167,SRS5024083,SRA913571,Victor Chang Cardiac Research Institute|Genome core,Victor Chang Cardiac Research Institute,2,0.93202,0.93602,0.11765,0.11618,0.732,0.73478,0.54315,0.5277,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Australia,2019-06-26,Adult,Adult,Heart,Cardiovascular System 52919,SRR9596463,SRX6362166,SRS5024083,SRP211964,PRJNA551130,Induction of adult cardiomyocyte renewal by Klf1,PRJNA551130,Other,In highly regenerative animals cardiac regeneration occurs innately through cardiomyocyte dedifferentiation and proliferation although the regenerative mechanisms remain unclear. This project contains raw sequencing data sets that were used to identify that klf1 a Kruppel like transcription factor essential for red blood cell development is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts,,,klf1 over expressing,,klf1,,strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:klf1 overexpressing|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish cardiac tissue,OE4 RNASEQ,OE4 RNASEQ,Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. RNA libraries were prepared 500ng input using the Kapa stranded RNA Seq kit using the oligo dT module to enrich for mRNA transcripts. Libraries were pooled to equimolar concentrations and sequenced on an Illumina HiSeq2500 paired end reads using v4 high output chemistry.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP211964,,,CCB6CANXX_5_180204_CFL--007_Other_GATCAG_R_180116_DAVHUM_LIB2500_M003_R1.fastq.gz CCB6CANXX_5_180204_CFL--007_Other_GATCAG_R_180116_DAVHUM_LIB2500_M003_R2.fastq.gz,fastq fastq,7295330500.0,29181322.0,CCB6CANXX 5 180204 CFL 007 Other GATCAG R 180116 DAVHUM LIB2500 M003 R1.fastq.gz,0:125 1:125,A:2032530437;C:1615271949;G:1627524722;T:2018148293;N:1855099,125,125,,,2032530437,1615271949,1627524722,2018148293,1855099,SRX6362166,SRS5024083,SRA913571,Victor Chang Cardiac Research Institute|Genome core,Victor Chang Cardiac Research Institute,2,0.9317,0.93743,0.11776,0.11755,0.73497,0.73888,0.51221,0.53503,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Australia,2019-06-26,Adult,Adult,Heart,Cardiovascular System 52920,SRR9596464,SRX6362165,SRS5024084,SRP211964,PRJNA551130,Induction of adult cardiomyocyte renewal by Klf1,PRJNA551130,Other,In highly regenerative animals cardiac regeneration occurs innately through cardiomyocyte dedifferentiation and proliferation although the regenerative mechanisms remain unclear. This project contains raw sequencing data sets that were used to identify that klf1 a Kruppel like transcription factor essential for red blood cell development is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts,,,WT,,WT,,strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:WT|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish cardiac tissue,WT1 RNASEQ,WT1 RNASEQ,Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. RNA libraries were prepared 500ng input using the Kapa stranded RNA Seq kit using the oligo dT module to enrich for mRNA transcripts. Libraries were pooled to equimolar concentrations and sequenced on an Illumina HiSeq2500 paired end reads using v4 high output chemistry.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP211964,,,CCB6CANXX_5_180204_CFL--008_Other_TAGCTT_R_180116_DAVHUM_LIB2500_M003_R1.fastq.gz CCB6CANXX_5_180204_CFL--008_Other_TAGCTT_R_180116_DAVHUM_LIB2500_M003_R2.fastq.gz,fastq fastq,7309496000.0,29237984.0,CCB6CANXX 5 180204 CFL 008 Other TAGCTT R 180116 DAVHUM LIB2500 M003 R1.fastq.gz,0:125 1:125,A:2038562638;C:1607745758;G:1627387822;T:2033916634;N:1883148,125,125,,,2038562638,1607745758,1627387822,2033916634,1883148,SRX6362165,SRS5024084,SRA913571,Victor Chang Cardiac Research Institute|Genome core,Victor Chang Cardiac Research Institute,2,0.94874,0.95324,0.08149,0.08128,0.79356,0.79575,0.58607,0.5765,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Australia,2019-06-26,Adult,Adult,Heart,Cardiovascular System 52921,SRR9596465,SRX6362164,SRS5024084,SRP211964,PRJNA551130,Induction of adult cardiomyocyte renewal by Klf1,PRJNA551130,Other,In highly regenerative animals cardiac regeneration occurs innately through cardiomyocyte dedifferentiation and proliferation although the regenerative mechanisms remain unclear. This project contains raw sequencing data sets that were used to identify that klf1 a Kruppel like transcription factor essential for red blood cell development is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts,,,WT,,WT,,strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:WT|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish cardiac tissue,WT2 RNASEQ,WT2 RNASEQ,Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. RNA libraries were prepared 500ng input using the Kapa stranded RNA Seq kit using the oligo dT module to enrich for mRNA transcripts. Libraries were pooled to equimolar concentrations and sequenced on an Illumina HiSeq2500 paired end reads using v4 high output chemistry.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP211964,,,CCB6CANXX_5_180204_CFL--009_Other_GGCTAC_R_180116_DAVHUM_LIB2500_M003_R1.fastq.gz CCB6CANXX_5_180204_CFL--009_Other_GGCTAC_R_180116_DAVHUM_LIB2500_M003_R2.fastq.gz,fastq fastq,6324176500.0,25296706.0,CCB6CANXX 5 180204 CFL 009 Other GGCTAC R 180116 DAVHUM LIB2500 M003 R1.fastq.gz,0:125 1:125,A:1766296515;C:1389598935;G:1403023173;T:1763639041;N:1618836,125,125,,,1766296515,1389598935,1403023173,1763639041,1618836,SRX6362164,SRS5024084,SRA913571,Victor Chang Cardiac Research Institute|Genome core,Victor Chang Cardiac Research Institute,2,0.94374,0.94892,0.09048,0.09084,0.78681,0.79066,0.57761,0.5755,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Australia,2019-06-26,Adult,Adult,Heart,Cardiovascular System 52922,SRR9596466,SRX6362163,SRS5024084,SRP211964,PRJNA551130,Induction of adult cardiomyocyte renewal by Klf1,PRJNA551130,Other,In highly regenerative animals cardiac regeneration occurs innately through cardiomyocyte dedifferentiation and proliferation although the regenerative mechanisms remain unclear. This project contains raw sequencing data sets that were used to identify that klf1 a Kruppel like transcription factor essential for red blood cell development is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts,,,WT,,WT,,strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:WT|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish cardiac tissue,WT3 RNASEQ,WT3 RNASEQ,Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. RNA libraries were prepared 500ng input using the Kapa stranded RNA Seq kit using the oligo dT module to enrich for mRNA transcripts. Libraries were pooled to equimolar concentrations and sequenced on an Illumina HiSeq2500 paired end reads using v4 high output chemistry.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP211964,,,CCB6CANXX_5_180204_CFL--010_Other_GTGGCC_R_180116_DAVHUM_LIB2500_M003_R1.fastq.gz CCB6CANXX_5_180204_CFL--010_Other_GTGGCC_R_180116_DAVHUM_LIB2500_M003_R2.fastq.gz,fastq fastq,7057191250.0,28228765.0,CCB6CANXX 5 180204 CFL 010 Other GTGGCC R 180116 DAVHUM LIB2500 M003 R1.fastq.gz,0:125 1:125,A:1983309478;C:1539045596;G:1559191474;T:1973857420;N:1787282,125,125,,,1983309478,1539045596,1559191474,1973857420,1787282,SRX6362163,SRS5024084,SRA913571,Victor Chang Cardiac Research Institute|Genome core,Victor Chang Cardiac Research Institute,2,0.94841,0.95328,0.08661,0.08555,0.78936,0.79243,0.58731,0.61025,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Australia,2019-06-26,Adult,Adult,Heart,Cardiovascular System 52923,SRR9596467,SRX6362162,SRS5024084,SRP211964,PRJNA551130,Induction of adult cardiomyocyte renewal by Klf1,PRJNA551130,Other,In highly regenerative animals cardiac regeneration occurs innately through cardiomyocyte dedifferentiation and proliferation although the regenerative mechanisms remain unclear. This project contains raw sequencing data sets that were used to identify that klf1 a Kruppel like transcription factor essential for red blood cell development is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts,,,WT,,WT,,strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:WT|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish cardiac tissue,WT4 RNASEQ,WT4 RNASEQ,Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. RNA libraries were prepared 500ng input using the Kapa stranded RNA Seq kit using the oligo dT module to enrich for mRNA transcripts. Libraries were pooled to equimolar concentrations and sequenced on an Illumina HiSeq2500 paired end reads using v4 high output chemistry.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP211964,,,CCB6CANXX_5_180204_CFL--011_Other_GTTTCG_R_180116_DAVHUM_LIB2500_M003_R1.fastq.gz CCB6CANXX_5_180204_CFL--011_Other_GTTTCG_R_180116_DAVHUM_LIB2500_M003_R2.fastq.gz,fastq fastq,6296219250.0,25184877.0,CCB6CANXX 5 180204 CFL 011 Other GTTTCG R 180116 DAVHUM LIB2500 M003 R1.fastq.gz,0:125 1:125,A:1758686297;C:1380462937;G:1398800047;T:1756652381;N:1617588,125,125,,,1758686297,1380462937,1398800047,1756652381,1617588,SRX6362162,SRS5024084,SRA913571,Victor Chang Cardiac Research Institute|Genome core,Victor Chang Cardiac Research Institute,2,0.94465,0.94985,0.09014,0.08958,0.78151,0.78472,0.58358,0.58306,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Australia,2019-06-26,Adult,Adult,Heart,Cardiovascular System