rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 29575,SRR27387418,SRX23063709,SRS20023731,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Combined Intestine,combined intestine 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:combined intestine replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T8 6,T8 6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A54_1.fastq.gz A54_2.fastq.gz,fastq fastq,8459790000.0,28199300.0,A54 1.fastq.gz,0:150 1:150,A:2287135948;C:1957145280;G:1959338891;T:2256075943;N:93938,150,150,,,2287135948,1957145280,1959338891,2256075943,93938,SRX23063709,SRS20023731,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92583,0.92422,0.06345,0.06263,0.75718,0.75757,0.52145,0.5361,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29576,SRR27387419,SRX23063708,SRS20023730,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Combined Intestine,combined intestine 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:combined intestine replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T8 5,T8 5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A53_1.fastq.gz A53_2.fastq.gz,fastq fastq,8302022700.0,27673409.0,A53 1.fastq.gz,0:150 1:150,A:2241277601;C:1925048164;G:1920142999;T:2215462687;N:91249,150,150,,,2241277601,1925048164,1920142999,2215462687,91249,SRX23063708,SRS20023730,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92711,0.92566,0.06314,0.06353,0.75221,0.75219,0.541,0.53579,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29577,SRR27387420,SRX23063707,SRS20023729,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Combined Intestine,combined intestine 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:combined intestine replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T8 4,T8 4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A49_1.fastq.gz A49_2.fastq.gz,fastq fastq,6939976800.0,23133256.0,A49 1.fastq.gz,0:150 1:150,A:1895013575;C:1589528661;G:1585890890;T:1869466351;N:77323,150,150,,,1895013575,1589528661,1585890890,1869466351,77323,SRX23063707,SRS20023729,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92632,0.92542,0.06323,0.06265,0.75588,0.75722,0.55002,0.55324,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29578,SRR27387421,SRX23063706,SRS20023728,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Combined Intestine,combined intestine 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:combined intestine replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T8 3,T8 3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A44_1.fastq.gz A44_2.fastq.gz,fastq fastq,9116379900.0,30387933.0,A44 1.fastq.gz,0:150 1:150,A:2462347514;C:2113718621;G:2112401730;T:2427812186;N:99849,150,150,,,2462347514,2113718621,2112401730,2427812186,99849,SRX23063706,SRS20023728,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.93154,0.92882,0.06321,0.06277,0.75499,0.75519,0.54224,0.542,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29579,SRR27387422,SRX23063705,SRS20023727,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Combined Intestine,combined intestine 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:combined intestine replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T8 2,T8 2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A43_1.fastq.gz A43_2.fastq.gz,fastq fastq,7681997100.0,25606657.0,A43 1.fastq.gz,0:150 1:150,A:2098281007;C:1758609887;G:1757658923;T:2067364339;N:82944,150,150,,,2098281007,1758609887,1757658923,2067364339,82944,SRX23063705,SRS20023727,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92257,0.92131,0.07154,0.07125,0.74769,0.74712,0.52352,0.51843,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29580,SRR27387423,SRX23063704,SRS20023726,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Combined Intestine,combined intestine 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:combined intestine replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T8 1,T8 1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A29_1.fastq.gz A29_2.fastq.gz,fastq fastq,8869294500.0,29564315.0,A29 1.fastq.gz,0:150 1:150,A:2411657291;C:2043119196;G:2038118093;T:2376336869;N:63051,150,150,,,2411657291,2043119196,2038118093,2376336869,63051,SRX23063704,SRS20023726,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.9293,0.93157,0.06846,0.06859,0.75138,0.75209,0.54348,0.54178,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29582,SRR27387425,SRX23063702,SRS20023724,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Nanoplastics Intestine,nps intestine 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:nps intestine replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T7 5,T7 5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A51_1.fastq.gz A51_2.fastq.gz,fastq fastq,8229865500.0,27432885.0,A51 1.fastq.gz,0:150 1:150,A:2239224965;C:1891494000;G:1890511233;T:2208544358;N:90944,150,150,,,2239224965,1891494000,1890511233,2208544358,90944,SRX23063702,SRS20023724,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.9282,0.92676,0.06786,0.06815,0.74566,0.74673,0.54961,0.55067,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29583,SRR27387426,SRX23063701,SRS20023723,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Nanoplastics Intestine,nps intestine 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:nps intestine replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T7 4,T7 4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A50_1.fastq.gz A50_2.fastq.gz,fastq fastq,8549274300.0,28497581.0,A50 1.fastq.gz,0:150 1:150,A:2321938070;C:1971518879;G:1967040023;T:2288683614;N:93714,150,150,,,2321938070,1971518879,1967040023,2288683614,93714,SRX23063701,SRS20023723,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.93231,0.93126,0.06361,0.06304,0.7527,0.75211,0.48289,0.48336,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29584,SRR27387427,SRX23063700,SRS20023722,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Nanoplastics Intestine,nps intestine 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:nps intestine replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T7 3,T7 3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A48_1.fastq.gz A48_2.fastq.gz,fastq fastq,8065026000.0,26883420.0,A48 1.fastq.gz,0:150 1:150,A:2177870889;C:1870003683;G:1870725132;T:2146337507;N:88789,150,150,,,2177870889,1870003683,1870725132,2146337507,88789,SRX23063700,SRS20023722,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92891,0.92753,0.05842,0.05778,0.71991,0.72009,0.51653,0.51675,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29585,SRR27387428,SRX23063699,SRS20023721,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Nanoplastics Intestine,nps intestine 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:nps intestine replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T7 2,T7 2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A42_1.fastq.gz A42_2.fastq.gz,fastq fastq,8391356700.0,27971189.0,A42 1.fastq.gz,0:150 1:150,A:2247673277;C:1962692430;G:1966871884;T:2214026582;N:92527,150,150,,,2247673277,1962692430,1966871884,2214026582,92527,SRX23063699,SRS20023721,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.93153,0.93045,0.05902,0.05934,0.75424,0.75546,0.548,0.54354,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29586,SRR27387429,SRX23063698,SRS20023720,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Nanoplastics Intestine,nps intestine 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:nps intestine replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T7 1,T7 1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A31_1.fastq.gz A31_2.fastq.gz,fastq fastq,8236557000.0,27455190.0,A31 1.fastq.gz,0:150 1:150,A:2204148101;C:1929010419;G:1925723147;T:2177582914;N:92419,150,150,,,2204148101,1929010419,1925723147,2177582914,92419,SRX23063698,SRS20023720,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.93739,0.93581,0.06216,0.06176,0.75057,0.75049,0.54176,0.53414,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29587,SRR27387430,SRX23063697,SRS20023719,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,6PPDQ Intestine,6ppdq intestine 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:6ppdq intestine replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T6 6,T6 6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A56_1.fastq.gz A56_2.fastq.gz,fastq fastq,6435464400.0,21451548.0,A56 1.fastq.gz,0:150 1:150,A:1748400934;C:1486171533;G:1481023797;T:1719795546;N:72590,150,150,,,1748400934,1486171533,1481023797,1719795546,72590,SRX23063697,SRS20023719,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92722,0.92559,0.0729,0.07288,0.74288,0.74343,0.53587,0.52589,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29588,SRR27387431,SRX23063696,SRS20023718,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,6PPDQ Intestine,6ppdq intestine 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:6ppdq intestine replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T6 5,T6 5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A40_1.fastq.gz A40_2.fastq.gz,fastq fastq,8749914000.0,29166380.0,A40 1.fastq.gz,0:150 1:150,A:2338416402;C:2051360445;G:2055480569;T:2304561447;N:95137,150,150,,,2338416402,2051360445,2055480569,2304561447,95137,SRX23063696,SRS20023718,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.94523,0.9504,0.03896,0.03887,0.80661,0.80728,0.46402,0.45996,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29589,SRR27387432,SRX23063695,SRS20023717,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,6PPDQ Intestine,6ppdq intestine 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:6ppdq intestine replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T6 4,T6 4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A39_1.fastq.gz A39_2.fastq.gz,fastq fastq,8140938300.0,27136461.0,A39 1.fastq.gz,0:150 1:150,A:2198617601;C:1888830697;G:1887283102;T:2166117857;N:89043,150,150,,,2198617601,1888830697,1887283102,2166117857,89043,SRX23063695,SRS20023717,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92636,0.92532,0.05883,0.0588,0.71689,0.71593,0.52397,0.5214,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29590,SRR27387433,SRX23063694,SRS20023716,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,6PPDQ Intestine,6ppdq intestine 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:6ppdq intestine replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T6 3,T6 3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A35_1.fastq.gz A35_2.fastq.gz,fastq fastq,6912319200.0,23041064.0,A35 1.fastq.gz,0:150 1:150,A:1895216825;C:1576630157;G:1574441195;T:1865953455;N:77568,150,150,,,1895216825,1576630157,1574441195,1865953455,77568,SRX23063694,SRS20023716,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92983,0.92816,0.072,0.07173,0.76601,0.76615,0.55997,0.55562,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29591,SRR27387434,SRX23063693,SRS20023715,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,6PPDQ Intestine,6ppdq intestine 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:6ppdq intestine replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T6 2,T6 2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A34_1.fastq.gz A34_2.fastq.gz,fastq fastq,7279802100.0,24266007.0,A34 1.fastq.gz,0:150 1:150,A:1989158951;C:1663891494;G:1668311413;T:1958359476;N:80766,150,150,,,1989158951,1663891494,1668311413,1958359476,80766,SRX23063693,SRS20023715,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92923,0.92655,0.06821,0.068,0.74042,0.74128,0.54366,0.54276,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29593,SRR27387436,SRX23063691,SRS20023713,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,6PPDQ Intestine,6ppdq intestine 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:6ppdq intestine replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T6 1,T6 1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A30_1.fastq.gz A30_2.fastq.gz,fastq fastq,7219947600.0,24066492.0,A30 1.fastq.gz,0:150 1:150,A:1901354175;C:1717600917;G:1716606554;T:1884305293;N:80661,150,150,,,1901354175,1717600917,1716606554,1884305293,80661,SRX23063691,SRS20023713,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.93375,0.93214,0.05513,0.05567,0.74759,0.74698,0.5475,0.54855,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29594,SRR27387437,SRX23063690,SRS20023712,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Control Intestine,control intestine 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:control intestine replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T5 6,T5 6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A46_1.fastq.gz A46_2.fastq.gz,fastq fastq,8509361100.0,28364537.0,A46 1.fastq.gz,0:150 1:150,A:2341600956;C:1932495972;G:1930461855;T:2304710926;N:91391,150,150,,,2341600956,1932495972,1930461855,2304710926,91391,SRX23063690,SRS20023712,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92542,0.92408,0.07033,0.06986,0.77234,0.77199,0.55246,0.55942,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29595,SRR27387438,SRX23063689,SRS20023711,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Control Intestine,control intestine 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:control intestine replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T5 5,T5 5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A45_1.fastq.gz A45_2.fastq.gz,fastq fastq,7856294100.0,26187647.0,A45 1.fastq.gz,0:150 1:150,A:2129077180;C:1813666476;G:1813868805;T:2099595491;N:86148,150,150,,,2129077180,1813666476,1813868805,2099595491,86148,SRX23063689,SRS20023711,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.93087,0.92866,0.06398,0.06358,0.75481,0.75597,0.55424,0.55289,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29596,SRR27387439,SRX23063688,SRS20023710,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Control Intestine,control intestine 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:control intestine replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T5 4,T5 4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A38_1.fastq.gz A38_2.fastq.gz,fastq fastq,8039208600.0,26797362.0,A38 1.fastq.gz,0:150 1:150,A:2201215819;C:1836215616;G:1833348507;T:2168337651;N:91007,150,150,,,2201215819,1836215616,1833348507,2168337651,91007,SRX23063688,SRS20023710,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.9219,0.91957,0.0702,0.06947,0.76343,0.76493,0.55355,0.54863,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29597,SRR27387440,SRX23063687,SRS20023709,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Control Intestine,control intestine 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:control intestine replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T5 3,T5 3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A37_1.fastq.gz A37_2.fastq.gz,fastq fastq,8253642600.0,27512142.0,A37 1.fastq.gz,0:150 1:150,A:2254958000;C:1889478281;G:1886099650;T:2223014303;N:92366,150,150,,,2254958000,1889478281,1886099650,2223014303,92366,SRX23063687,SRS20023709,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92632,0.92477,0.06268,0.06279,0.76719,0.76682,0.53773,0.53566,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29598,SRR27387441,SRX23063686,SRS20023708,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Control Intestine,control intestine 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:control intestine replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T5 2,T5 2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A33_1.fastq.gz A33_2.fastq.gz,fastq fastq,8276959500.0,27589865.0,A33 1.fastq.gz,0:150 1:150,A:2244800716;C:1909951370;G:1909130637;T:2212988991;N:87786,150,150,,,2244800716,1909951370,1909130637,2212988991,87786,SRX23063686,SRS20023708,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.93248,0.93089,0.06402,0.06475,0.701,0.7012,0.53585,0.53722,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 29599,SRR27387442,SRX23063685,SRS20023707,SRP480720,PRJNA1059246,Mixture toxicity of 6PPD quinone and polystyrene nanoplastics in zebrafish,PRJNA1059246,Other,Tyre wear particles are the second largest source of microplastics in the ocean following abrasion of synthetic fibres. In addition to the particles themselves TWPs contain many harmful chemicals including 6PPD. This chemical reacts with atmospheric ozone and forms the toxic compound 6PPD quinone which poses a danger to aquatic life. There is a knowledge gap in understanding risks associated with thecombined toxicity of nanoplastics and 6PPDq. The present study aimed to investigate the toxicity of NPs and 6PPDq on adult zebrafish. However we found significant hyperlocomotion in zebrafish exposed to 6PPDq and this effect was even more substantial post co exposure with PSNPs. The more pronounced toxic response in coexposed zebrafish was also observed at the transcriptomic level. KEGG pathways analysis identified mitochondrial dysfunction in the intestine and metabolic disturbances in the liver. These results highlight the significance of mixture toxicity when studying the effects of NPs and associated chemicals like 6PPDq.,,,,Control Intestine,control intestine 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:16 weeks|dev stage:adult|sex:male|tissue:intestine|biomaterial provider:not applicable|birth date:not applicable|birth location:not applicable|breeding history:not applicable|breeding method:not applicable|cell line:not applicable|cell subtype:not applicable|cell type:not applicable|collected by:Shubham Varshney|collection date:2023 06|culture collection:not applicable|death date:not applicable|disease:not applicable|disease stg:not applicable|genotype:not applicable|geo loc name:not applicable|growth protocol:not applicable|health state:not applicable|isolation source:not applicable|lat lon:not applicable|phenotype:not applicable|sample type:not applicable|specimen voucher:not applicable|store cond:not applicable|stud book number:not applicable|treatment:control intestine replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,T5 1,T5 1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,NextSeq 550,,SRP480720,,,A32_1.fastq.gz A32_2.fastq.gz,fastq fastq,7955418900.0,26518063.0,A32 1.fastq.gz,0:150 1:150,A:2236485440;C:1768380410;G:1765867470;T:2184596828;N:88752,150,150,,,2236485440,1768380410,1765867470,2184596828,88752,SRX23063685,SRS20023707,SRA1777576,Nord University|Faculty of Biosciences and Aquaculture,Nord University,2,0.92662,0.92301,0.071,0.07061,0.75207,0.75158,0.53095,0.54507,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2023-12-30,Adult,Adult,Gut,Digestive System 55951,SRR10827995,SRX7501389,SRS5942789,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR011,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate10|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR011,ZR011,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR011_S00_L009_R2_001.fastq.gz lane9-ZR011_S00_L009_R1_001.fastq.gz,fastq fastq,9665147298.0,32003799.0,lane9 ZR011 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2665223251;C:2186863125;G:2211599706;T:2600518585;N:942631,151,151,,,2665223251,2186863125,2211599706,2600518585,942631,SRX7501389,SRS5942789,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.85775,0.85388,0.06267,0.06297,0.78766,0.79097,0.54825,0.54162,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55952,SRR10827996,SRX7501388,SRS5942788,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR010,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate9|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR010,ZR010,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR010_S00_L009_R2_001.fastq.gz lane9-ZR010_S00_L009_R1_001.fastq.gz,fastq fastq,10313891618.0,34151959.0,lane9 ZR010 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2810497988;C:2363542718;G:2386667646;T:2752188319;N:994947,151,151,,,2810497988,2363542718,2386667646,2752188319,994947,SRX7501388,SRS5942788,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.86439,0.85421,0.05536,0.05624,0.78378,0.78847,0.5643,0.5428,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55953,SRR10827997,SRX7501387,SRS5942787,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR009,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate8|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR009,ZR009,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR009_S00_L009_R2_001.fastq.gz lane9-ZR009_S00_L009_R1_001.fastq.gz,fastq fastq,8998700946.0,29797023.0,lane9 ZR009 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2553315628;C:1959457429;G:1980318504;T:2504726656;N:882729,151,151,,,2553315628,1959457429,1980318504,2504726656,882729,SRX7501387,SRS5942787,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.80444,0.8015,0.08208,0.08274,0.78634,0.78813,0.56791,0.56822,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55954,SRR10827998,SRX7501386,SRS5942786,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR008,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate7|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR008,ZR008,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR008_S00_L009_R1_001.fastq.gz lane9-ZR008_S00_L009_R2_001.fastq.gz,fastq fastq,9206500200.0,30485100.0,lane9 ZR008 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2571733836;C:2048238935;G:2081481702;T:2504158985;N:886742,151,151,,,2571733836,2048238935,2081481702,2504158985,886742,SRX7501386,SRS5942786,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.84315,0.84243,0.06679,0.068,0.79026,0.79263,0.54865,0.52814,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55955,SRR10827999,SRX7501385,SRS5942785,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR006,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR006,ZR006,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR006_S00_L009_R1_001.fastq.gz lane9-ZR006_S00_L009_R2_001.fastq.gz,fastq fastq,9469616190.0,31356345.0,lane9 ZR006 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2598567090;C:2156360016;G:2166235031;T:2547531313;N:922740,151,151,,,2598567090,2156360016,2166235031,2547531313,922740,SRX7501385,SRS5942785,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.86997,0.86634,0.06206,0.06323,0.7853,0.7877,0.5039,0.48672,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55956,SRR10828000,SRX7501384,SRS5942783,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR005,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR005,ZR005,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR005_S00_L009_R1_001.fastq.gz lane9-ZR005_S00_L009_R2_001.fastq.gz,fastq fastq,8265125732.0,27367966.0,lane9 ZR005 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2271031367;C:1878130541;G:1893994523;T:2221168810;N:800491,151,151,,,2271031367,1878130541,1893994523,2221168810,800491,SRX7501384,SRS5942783,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.87646,0.7655,0.06913,0.06038,0.79401,0.80915,0.54875,0.54807,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55957,SRR10828001,SRX7501383,SRS5942784,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR004,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR004,ZR004,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR004_S00_L009_R1_001.fastq.gz lane9-ZR004_S00_L009_R2_001.fastq.gz,fastq fastq,9015407586.0,29852343.0,lane9 ZR004 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2508921769;C:2018051929;G:2030281337;T:2457279135;N:873416,151,151,,,2508921769,2018051929,2030281337,2457279135,873416,SRX7501383,SRS5942784,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.84988,0.83488,0.06628,0.06644,0.77344,0.77843,0.55247,0.5423,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55958,SRR10828002,SRX7501382,SRS5942782,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR022,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate21|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR022,ZR022,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR022_S00_L009_R1_001.fastq.gz lane9-ZR022_S00_L009_R2_001.fastq.gz,fastq fastq,13741876102.0,45502901.0,lane9 ZR022 S00 L009 R1 001.fastq.gz,0:151 1:151,A:3931077197;C:2931142966;G:2955027469;T:3923313652;N:1314818,151,151,,,3931077197,2931142966,2955027469,3923313652,1314818,SRX7501382,SRS5942782,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.88334,0.87862,0.09332,0.09391,0.77711,0.78113,0.55947,0.54508,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55959,SRR10828003,SRX7501381,SRS5942781,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR003,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR003,ZR003,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR003_S00_L009_R1_001.fastq.gz lane9-ZR003_S00_L009_R2_001.fastq.gz,fastq fastq,11201243420.0,37090210.0,lane9 ZR003 S00 L009 R1 001.fastq.gz,0:151 1:151,A:3127283035;C:2496207030;G:2513262555;T:3063404560;N:1086240,151,151,,,3127283035,2496207030,2513262555,3063404560,1086240,SRX7501381,SRS5942781,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.86792,0.8632,0.06784,0.06862,0.78867,0.79308,0.53988,0.52892,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55960,SRR10828004,SRX7501380,SRS5942780,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR021,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate20|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR021,ZR021,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR021_S00_L009_R1_001.fastq.gz lane9-ZR021_S00_L009_R2_001.fastq.gz,fastq fastq,10099985924.0,33443662.0,lane9 ZR021 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2774168141;C:2288982049;G:2309143011;T:2726709515;N:983208,151,151,,,2774168141,2288982049,2309143011,2726709515,983208,SRX7501380,SRS5942780,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.86982,0.86834,0.07188,0.07308,0.78717,0.78987,0.5574,0.5503,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55961,SRR10828005,SRX7501379,SRS5942779,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR020,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate19|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR020,ZR020,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR020_S00_L009_R1_001.fastq.gz lane9-ZR020_S00_L009_R2_001.fastq.gz,fastq fastq,10454972428.0,34619114.0,lane9 ZR020 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2839651351;C:2416116684;G:2429373737;T:2768795024;N:1035632,151,151,,,2839651351,2416116684,2429373737,2768795024,1035632,SRX7501379,SRS5942779,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.86093,0.86611,0.03397,0.03484,0.83104,0.83327,0.30434,0.30026,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55962,SRR10828006,SRX7501378,SRS5942778,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR019,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate18|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR019,ZR019,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR019_S00_L009_R2_001.fastq.gz lane9-ZR019_S00_L009_R1_001.fastq.gz,fastq fastq,10719453458.0,35494879.0,lane9 ZR019 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2914826334;C:2463706695;G:2484150600;T:2855735087;N:1034742,151,151,,,2914826334,2463706695,2484150600,2855735087,1034742,SRX7501378,SRS5942778,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.87668,0.87454,0.05478,0.05538,0.78884,0.7906,0.53897,0.5064,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55963,SRR10828007,SRX7501377,SRS5942777,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR018,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate17|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR018,ZR018,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR018_S00_L009_R1_001.fastq.gz lane9-ZR018_S00_L009_R2_001.fastq.gz,fastq fastq,6358250620.0,21053810.0,lane9 ZR018 S00 L009 R1 001.fastq.gz,0:151 1:151,A:1669813509;C:1524057927;G:1530947497;T:1632824279;N:607408,151,151,,,1669813509,1524057927,1530947497,1632824279,607408,SRX7501377,SRS5942777,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.89698,0.89547,0.05683,0.05916,0.81848,0.82089,0.56455,0.54732,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55964,SRR10828008,SRX7501376,SRS5942776,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR017,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate16|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR017,ZR017,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR017_S00_L009_R1_001.fastq.gz lane9-ZR017_S00_L009_R2_001.fastq.gz,fastq fastq,8141425324.0,26958362.0,lane9 ZR017 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2210048052;C:1874541787;G:1892972101;T:2163066000;N:797384,151,151,,,2210048052,1874541787,1892972101,2163066000,797384,SRX7501376,SRS5942776,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.8799,0.75762,0.05488,0.04911,0.78587,0.80105,0.5506,0.52986,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55965,SRR10828009,SRX7501375,SRS5942775,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR016,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate15|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR016,ZR016,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR016_S00_L009_R1_001.fastq.gz lane9-ZR016_S00_L009_R2_001.fastq.gz,fastq fastq,6614210116.0,21901358.0,lane9 ZR016 S00 L009 R1 001.fastq.gz,0:151 1:151,A:1784851559;C:1535505997;G:1547676414;T:1745543179;N:632967,151,151,,,1784851559,1535505997,1547676414,1745543179,632967,SRX7501375,SRS5942775,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.90312,0.89783,0.0713,0.07131,0.79127,0.79579,0.54495,0.54771,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55966,SRR10828010,SRX7501374,SRS5942774,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR015,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate14|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR015,ZR015,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR015_S00_L009_R1_001.fastq.gz lane9-ZR015_S00_L009_R2_001.fastq.gz,fastq fastq,11286865554.0,37373727.0,lane9 ZR015 S00 L009 R1 001.fastq.gz,0:151 1:151,A:3141361472;C:2517784036;G:2559469326;T:3067139151;N:1111569,151,151,,,3141361472,2517784036,2559469326,3067139151,1111569,SRX7501374,SRS5942774,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.83638,0.834,0.06549,0.06651,0.78305,0.78524,0.55623,0.54699,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55967,SRR10828011,SRX7501373,SRS5942773,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR014,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate13|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR014,ZR014,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR014_S00_L009_R1_001.fastq.gz lane9-ZR014_S00_L009_R2_001.fastq.gz,fastq fastq,7845860038.0,25979669.0,lane9 ZR014 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2154729734;C:1778438216;G:1807077828;T:2104855748;N:758512,151,151,,,2154729734,1778438216,1807077828,2104855748,758512,SRX7501373,SRS5942773,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.86906,0.86569,0.06254,0.06319,0.78886,0.79336,0.54972,0.51512,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55968,SRR10828012,SRX7501372,SRS5942772,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR013,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate12|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR013,ZR013,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR013_S00_L009_R1_001.fastq.gz lane9-ZR013_S00_L009_R2_001.fastq.gz,fastq fastq,6510195276.0,21556938.0,lane9 ZR013 S00 L009 R1 001.fastq.gz,0:151 1:151,A:1814210714;C:1453505783;G:1467029872;T:1774830373;N:618534,151,151,,,1814210714,1453505783,1467029872,1774830373,618534,SRX7501372,SRS5942772,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.85325,0.85017,0.06548,0.06721,0.79815,0.79939,0.55953,0.53689,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55969,SRR10828013,SRX7501371,SRS5942771,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR012,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate11|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR012,ZR012,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR012_S00_L009_R1_001.fastq.gz lane9-ZR012_S00_L009_R2_001.fastq.gz,fastq fastq,6797139368.0,22507084.0,lane9 ZR012 S00 L009 R1 001.fastq.gz,0:151 1:151,A:1862619574;C:1544924953;G:1561143823;T:1827800623;N:650395,151,151,,,1862619574,1544924953,1561143823,1827800623,650395,SRX7501371,SRS5942771,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.87394,0.86984,0.05857,0.05973,0.78267,0.78535,0.56602,0.53678,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55970,SRR10828014,SRX7501370,SRS5942769,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR002,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR002,ZR002,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR002_S00_L009_R1_001.fastq.gz lane9-ZR002_S00_L009_R2_001.fastq.gz,fastq fastq,12048079506.0,39894303.0,lane9 ZR002 S00 L009 R1 001.fastq.gz,0:151 1:151,A:3325434575;C:2709618615;G:2744517142;T:3267339636;N:1169538,151,151,,,3325434575,2709618615,2744517142,3267339636,1169538,SRX7501370,SRS5942769,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.86595,0.86133,0.06795,0.06891,0.79316,0.79711,0.51314,0.50071,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 55971,SRR10828015,SRX7501369,SRS5942770,SRP239446,PRJNA598916,Zebrafish intestinal gene expression,PRJNA598916,Other,An analysis of zebrafish intestinal gene expression,,,,,ZR001,,strain:5D|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|dev stage:Adult|sex:male|tissue:whole intestine|replicate:replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio: adult 5D intestine,ZR001,ZR001,Illumina Paired end sequencing,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP239446,,,lane9-ZR001_S00_L009_R1_001.fastq.gz lane9-ZR001_S00_L009_R2_001.fastq.gz,fastq fastq,8481414206.0,28084153.0,lane9 ZR001 S00 L009 R1 001.fastq.gz,0:151 1:151,A:2392757541;C:1869273565;G:1884123300;T:2334437148;N:822652,151,151,,,2392757541,1869273565,1884123300,2334437148,822652,SRX7501369,SRS5942770,SRA1020118,Oregon State University|Microbiology,Oregon State University,2,0.79806,0.66202,0.06966,0.05946,0.7809,0.79849,0.52853,0.52572,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,United States,2020-01-04,Adult,Adult,Gut,Digestive System 62742,SRR13360748,SRX9785053,SRS7972286,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,macrogard,macrogard2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:macrogardPM replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,PM2,PM2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,pm2_S8_R1_001.fastq.gz,fastq,3517898944.0,46288144.0,pm2 S8 R1 001.fastq.gz,0:76 1:0,A:914616261;C:828363626;G:805674434;T:969167482;N:77141,76,0,,,914616261,828363626,805674434,969167482,77141,SRX9785053,SRS7972286,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.94008,,0.0747,,0.71394,,0.51572,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62743,SRR13360749,SRX9785052,SRS7972285,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,macrogard,macrogard1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:macrogardPM replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,PM1,PM1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,pm1_S7_R1_001.fastq.gz,fastq,1974822380.0,25984505.0,pm1 S7 R1 001.fastq.gz,0:76 1:0,A:561606319;C:416363486;G:408486827;T:588324386;N:41362,76,0,,,561606319,416363486,408486827,588324386,41362,SRX9785052,SRS7972285,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.9274,,0.16109,,0.74351,,0.57108,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62744,SRR13360750,SRX9785051,SRS7972284,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,soybean,soybean4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:soybeanCP replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CP4,CP4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cp4_S4_R1_001.fastq.gz,fastq,2913402316.0,38334241.0,cp4 S4 R1 001.fastq.gz,0:76 1:0,A:791131658;C:648321809;G:621173454;T:852725371;N:50024,76,0,,,791131658,648321809,621173454,852725371,50024,SRX9785051,SRS7972284,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93191,,0.1275,,0.7333,,0.55298,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62745,SRR13360751,SRX9785050,SRS7972283,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,soybean,soybean3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:soybeanCP replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CP3,CP3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cp3_S6_R1_001.fastq.gz,fastq,2648220684.0,34845009.0,cp3 S6 R1 001.fastq.gz,0:76 1:0,A:697194378;C:597685138;G:585283565;T:767999519;N:58084,76,0,,,697194378,597685138,585283565,767999519,58084,SRX9785050,SRS7972283,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93629,,0.1048,,0.7349,,0.54364,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62746,SRR13360752,SRX9785049,SRS7972282,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,soybean,soybean2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:soybeanCP replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CP2,CP2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cp2_S5_R1_001.fastq.gz,fastq,2339410872.0,30781722.0,cp2 S5 R1 001.fastq.gz,0:76 1:0,A:620056592;C:539298564;G:520500416;T:659503250;N:52050,76,0,,,620056592,539298564,520500416,659503250,52050,SRX9785049,SRS7972282,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93909,,0.0916,,0.70942,,0.51709,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62747,SRR13360753,SRX9785048,SRS7972281,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,soybean,soybean1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:soybeanCP replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CP1,CP1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cp1_S4_R1_001.fastq.gz,fastq,2271445592.0,29887442.0,cp1 S4 R1 001.fastq.gz,0:76 1:0,A:620595380;C:508382666;G:499686333;T:642731924;N:49289,76,0,,,620595380,508382666,499686333,642731924,49289,SRX9785048,SRS7972281,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93497,,0.1129,,0.71127,,0.50787,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62748,SRR13360754,SRX9785047,SRS7972280,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,control,control4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:controlCZ replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CZ4,CZ4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cz4_S1_R1_001.fastq.gz,fastq,2772612848.0,36481748.0,cz4 S1 R1 001.fastq.gz,0:76 1:0,A:753409223;C:625962831;G:590503241;T:802690640;N:46913,76,0,,,753409223,625962831,590503241,802690640,46913,SRX9785047,SRS7972280,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.92942,,0.15362,,0.62889,,0.51867,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62749,SRR13360755,SRX9785046,SRS7972279,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,control,control3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:controlCZ replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CZ3,CZ3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cz3_S3_R1_001.fastq.gz,fastq,2424287672.0,31898522.0,cz3 S3 R1 001.fastq.gz,0:76 1:0,A:629966430;C:571438897;G:558947157;T:663881953;N:53235,76,0,,,629966430,571438897,558947157,663881953,53235,SRX9785046,SRS7972279,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.94199,,0.04836,,0.72218,,0.48478,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62750,SRR13360756,SRX9785045,SRS7972278,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,macrogard,macrogard4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:macrogardPM replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,PM4,PM4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,pm4_S7_R1_001.fastq.gz,fastq,2901513560.0,38177810.0,pm4 S7 R1 001.fastq.gz,0:76 1:0,A:737189471;C:698799076;G:664901115;T:800575145;N:48753,76,0,,,737189471,698799076,664901115,800575145,48753,SRX9785045,SRS7972278,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.95173,,0.04367,,0.74089,,0.50116,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62751,SRR13360757,SRX9785044,SRS7972277,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,macrogard,macrogard3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:macrogardPM replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,PM3,PM3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,pm3_S9_R1_001.fastq.gz,fastq,3148390744.0,41426194.0,pm3 S9 R1 001.fastq.gz,0:76 1:0,A:834545372;C:725073648;G:704241250;T:884462724;N:67750,76,0,,,834545372,725073648,704241250,884462724,67750,SRX9785044,SRS7972277,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.94256,,0.09248,,0.72099,,0.52303,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62752,SRR13360758,SRX9785043,SRS7972276,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,control,control2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:controlCZ replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CZ2,CZ2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cz2_S2_R1_001.fastq,fastq,2207708116.0,29048791.0,cz2 S2 R1 001.fastq,0:76 1:0,A:614048827;C:488071931;G:474165427;T:631372646;N:49285,76,0,,,614048827,488071931,474165427,631372646,49285,SRX9785043,SRS7972276,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93271,,0.12538,,0.71733,,0.53166,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62753,SRR13360759,SRX9785042,SRS7972275,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,control,control1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:controlCZ replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CZ1,CZ1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cz1_S1_R1_001.fastq.gz,fastq,2264115696.0,29790996.0,cz1 S1 R1 001.fastq.gz,0:76 1:0,A:619936364;C:496981021;G:501038961;T:646109550;N:49800,76,0,,,619936364,496981021,501038961,646109550,49800,SRX9785042,SRS7972275,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93737,,0.1307,,0.73003,,0.55416,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 66094,SRR15910149,SRX12200592,SRS10176883,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC4,HC4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_5_S5_R1_001.fastq.gz,fastq,1878287660.0,24877927.0,high 5 S5 R1 001.fastq.gz,0:75.50 1:0,A:476823620;C:439449647;G:429501262;T:532480837;N:32294,75,0,,,476823620,439449647,429501262,532480837,32294,SRX12200592,SRS10176883,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94553,,0.08147,,0.74582,,0.53024,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66095,SRR15910150,SRX12200591,SRS10176881,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC3,HC3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_4_S4_R1_001.fastq.gz,fastq,1888383069.0,25020396.0,high 4 S4 R1 001.fastq.gz,0:75.47 1:0,A:503511674;C:424269464;G:408669727;T:551898358;N:33846,75,0,,,503511674,424269464,408669727,551898358,33846,SRX12200591,SRS10176881,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93958,,0.1137,,0.74472,,0.52962,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66096,SRR15910151,SRX12200590,SRS10176882,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC2,HC2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_3_S6_R1_001.fastq.gz,fastq,2337788390.0,31029913.0,high 3 S6 R1 001.fastq.gz,0:75.34 1:0,A:586705132;C:555534330;G:547485592;T:645264200;N:2799136,75,0,,,586705132,555534330,547485592,645264200,2799136,SRX12200590,SRS10176882,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94651,,0.08395,,0.67872,,0.51496,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66097,SRR15910152,SRX12200589,SRS10176880,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC1,HC1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_1_S4_R1_001.fastq.gz,fastq,2390465384.0,31789893.0,high 1 S4 R1 001.fastq.gz,0:75.20 1:0,A:599574460;C:559752099;G:546456599;T:677639257;N:7042969,75,0,,,599574460,559752099,546456599,677639257,7042969,SRX12200589,SRS10176880,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94326,,0.09122,,0.73892,,0.52413,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66098,SRR15910153,SRX12200588,SRS10176878,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC6,LC6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_6_S3_R1_001.fastq.gz,fastq,1915028032.0,25430699.0,control 6 S3 R1 001.fastq.gz,0:75.30 1:0,A:502285547;C:435337245;G:424726824;T:549290123;N:3388293,75,0,,,502285547,435337245,424726824,549290123,3388293,SRX12200588,SRS10176878,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.92666,,0.1269,,0.74968,,0.54447,,73,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66099,SRR15910154,SRX12200587,SRS10176877,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC5,LC5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_5_S2_R1_001.fastq.gz,fastq,2327013759.0,30824465.0,control 5 S2 R1 001.fastq.gz,0:75.49 1:0,A:590955916;C:541856449;G:529786509;T:664374176;N:40709,75,0,,,590955916,541856449,529786509,664374176,40709,SRX12200587,SRS10176877,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94087,,0.09604,,0.74489,,0.53048,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66100,SRR15910155,SRX12200586,SRS10176879,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC4,LC4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_4_S1_R1_001.fastq.gz,fastq,1559648966.0,20660136.0,control 4 S1 R1 001.fastq.gz,0:75.49 1:0,A:398086562;C:359082782;G:356015350;T:446436847;N:27425,75,0,,,398086562,359082782,356015350,446436847,27425,SRX12200586,SRS10176879,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93707,,0.10017,,0.73464,,0.52715,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66101,SRR15910156,SRX12200585,SRS10176876,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS6,SS6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_6_S15_R1_001.fastq.gz,fastq,2431012086.0,32275098.0,sim 6 S15 R1 001.fastq.gz,0:75.32 1:0,A:647191218;C:551606901;G:529110857;T:703050921;N:52189,75,0,,,647191218,551606901,529110857,703050921,52189,SRX12200585,SRS10176876,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93331,,0.1165,,0.75268,,0.52753,,74,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66102,SRR15910157,SRX12200584,SRS10176875,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS5,SS5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_5_S14_R1_001.fastq.gz,fastq,1902101028.0,25201273.0,sim 5 S14 R1 001.fastq.gz,0:75.48 1:0,A:482206150;C:442300454;G:433280346;T:544280250;N:33828,75,0,,,482206150,442300454,433280346,544280250,33828,SRX12200584,SRS10176875,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93441,,0.09457,,0.72431,,0.53047,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66103,SRR15910158,SRX12200583,SRS10176874,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS4,SS4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_4_S13_R1_001.fastq.gz,fastq,1887931329.0,25021534.0,sim 4 S13 R1 001.fastq.gz,0:75.45 1:0,A:483691629;C:436592360;G:424733601;T:542880751;N:32988,75,0,,,483691629,436592360,424733601,542880751,32988,SRX12200583,SRS10176874,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.92435,,0.09393,,0.76039,,0.54018,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66104,SRR15910159,SRX12200582,SRS10176873,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS3,SS3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_3_S15_R1_001.fastq.gz,fastq,2278093448.0,30193199.0,sim 3 S15 R1 001.fastq.gz,0:75.45 1:0,A:575276152;C:529447047;G:521207330;T:650819961;N:1342958,75,0,,,575276152,529447047,521207330,650819961,1342958,SRX12200582,SRS10176873,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94093,,0.087,,0.73876,,0.53488,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66105,SRR15910160,SRX12200581,SRS10176872,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS2,SS2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_2_S14_R1_001.fastq.gz,fastq,2543469673.0,33741391.0,sim 2 S14 R1 001.fastq.gz,0:75.38 1:0,A:667279341;C:584937962;G:562098766;T:726456492;N:2697112,75,0,,,667279341,584937962,562098766,726456492,2697112,SRX12200581,SRS10176872,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93369,,0.11515,,0.72283,,0.51172,,74,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66106,SRR15910161,SRX12200580,SRS10176871,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS1,SS1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_1_S13_R1_001.fastq.gz,fastq,1663748041.0,22080724.0,sim 1 S13 R1 001.fastq.gz,0:75.35 1:0,A:424766717;C:386318148;G:378172921;T:472190364;N:2299891,75,0,,,424766717,386318148,378172921,472190364,2299891,SRX12200580,SRS10176871,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93979,,0.08815,,0.74194,,0.52705,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66107,SRR15910162,SRX12200579,SRS10176870,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG6,OG6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_6_S12_R1_001.fastq.gz,fastq,1950238266.0,25861117.0,oat 6 S12 R1 001.fastq.gz,0:75.41 1:0,A:502273927;C:456918053;G:438368291;T:552640342;N:37653,75,0,,,502273927,456918053,438368291,552640342,37653,SRX12200579,SRS10176870,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93318,,0.08879,,0.74901,,0.54257,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66108,SRR15910163,SRX12200578,SRS10176869,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG5,OG5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_5_S11_R1_001.fastq.gz,fastq,1818990592.0,24148417.0,oat 5 S11 R1 001.fastq.gz,0:75.33 1:0,A:460795176;C:423681740;G:415715028;T:516509914;N:2288734,75,0,,,460795176,423681740,415715028,516509914,2288734,SRX12200578,SRS10176869,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93555,,0.09413,,0.73732,,0.54828,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66109,SRR15910164,SRX12200577,SRS10176868,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG4,OG4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_4_S10_R1_001.fastq.gz,fastq,1734135458.0,22972939.0,oat 4 S10 R1 001.fastq.gz,0:75.49 1:0,A:444047928;C:402248080;G:393083056;T:494726063;N:30331,75,0,,,444047928,402248080,393083056,494726063,30331,SRX12200577,SRS10176868,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93619,,0.09257,,0.75635,,0.54807,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66110,SRR15910165,SRX12200576,SRS10176867,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC3,LC3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_3_S3_R1_001.fastq.gz,fastq,2544827138.0,33810050.0,control 3 S3 R1 001.fastq.gz,0:75.27 1:0,A:649235065;C:587469807;G:574700155;T:728104191;N:5317920,75,0,,,649235065,587469807,574700155,728104191,5317920,SRX12200576,SRS10176867,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93445,,0.09084,,0.75471,,0.53891,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66111,SRR15910166,SRX12200575,SRS10176866,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG3,OG3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_3_S12_R1_001.fastq.gz,fastq,2676109047.0,35499063.0,oat 3 S12 R1 001.fastq.gz,0:75.39 1:0,A:668562223;C:624794464;G:608983398;T:770847910;N:2921052,75,0,,,668562223,624794464,608983398,770847910,2921052,SRX12200575,SRS10176866,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.95624,,0.07731,,0.78368,,0.5561,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66112,SRR15910167,SRX12200574,SRS10176865,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG2,OG2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_2_S11_R1_001.fastq.gz,fastq,1920714201.0,25464331.0,oat 2 S11 R1 001.fastq.gz,0:75.43 1:0,A:489513738;C:448024192;G:438816907;T:542892434;N:1466930,75,0,,,489513738,448024192,438816907,542892434,1466930,SRX12200574,SRS10176865,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94472,,0.08495,,0.73766,,0.54149,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66113,SRR15910168,SRX12200573,SRS10176864,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG1,OG1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_1_S10_R1_001.fastq.gz,fastq,2171014631.0,28803940.0,oat 1 S10 R1 001.fastq.gz,0:75.37 1:0,A:550611852;C:504793219;G:494893735;T:618143286;N:2572539,75,0,,,550611852,504793219,494893735,618143286,2572539,SRX12200573,SRS10176864,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94056,,0.089,,0.74142,,0.53823,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66114,SRR15910169,SRX12200572,SRS10176863,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG6,AG6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_6_S12_R1_001.fastq.gz,fastq,2098747142.0,27807858.0,alga 6 S12 R1 001.fastq.gz,0:75.47 1:0,A:534561461;C:494167859;G:489052359;T:580927807;N:37656,75,0,,,534561461,494167859,489052359,580927807,37656,SRX12200572,SRS10176863,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94831,,0.08427,,0.73292,,0.52842,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66115,SRR15910170,SRX12200571,SRS10176862,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG5,AG5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_5_S11_R1_001.fastq.gz,fastq,1763481116.0,23363097.0,alga 5 S11 R1 001.fastq.gz,0:75.48 1:0,A:447338889;C:412970655;G:414050708;T:489089552;N:31312,75,0,,,447338889,412970655,414050708,489089552,31312,SRX12200571,SRS10176862,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94349,,0.08062,,0.739,,0.52999,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66116,SRR15910171,SRX12200570,SRS10176861,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG4,AG4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_4_S10_R1_001.fastq.gz,fastq,1879826054.0,24897114.0,alga 4 S10 R1 001.fastq.gz,0:75.50 1:0,A:470066955;C:443235235;G:435473614;T:531017855;N:32395,75,0,,,470066955,443235235,435473614,531017855,32395,SRX12200570,SRS10176861,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94546,,0.09326,,0.65845,,0.52459,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66117,SRR15910172,SRX12200569,SRS10176858,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG3,AG3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_3_S12_R1_001.fastq.gz,fastq,2171152050.0,28804773.0,alga 3 S12 R1 001.fastq.gz,0:75.37 1:0,A:577878327;C:486896436;G:474333381;T:629132016;N:2911890,75,0,,,577878327,486896436,474333381,629132016,2911890,SRX12200569,SRS10176858,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.9263,,0.13343,,0.74308,,0.54791,,74,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66118,SRR15910173,SRX12200568,SRS10176857,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG2,AG2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_2_S11_R1_001.fastq.gz,fastq,2667876981.0,35347747.0,alga 2 S11 R1 001.fastq.gz,0:75.48 1:0,A:608681566;C:714247949;G:658536241;T:684669115;N:1742110,75,0,,,608681566,714247949,658536241,684669115,1742110,SRX12200568,SRS10176857,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.9577,,0.13465,,0.77425,,0.60177,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66119,SRR15910174,SRX12200567,SRS10176859,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG1,AG1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_1_S10_R_001.fastq.gz,fastq,2947209259.0,39094391.0,alga 1 S10 R 001.fastq.gz,0:75.39 1:0,A:769302900;C:671912702;G:657628697;T:845634633;N:2730327,75,0,,,769302900,671912702,657628697,845634633,2730327,SRX12200567,SRS10176859,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93867,,0.08991,,0.74272,,0.53283,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66120,SRR15910175,SRX12200566,SRS10176855,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC5,HC5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_6_S6_R1_001.fastq.gz,fastq,1822201784.0,24137941.0,high 6 S6 R1 001.fastq.gz,0:75.49 1:0,A:461483337;C:426648920;G:419516035;T:514521907;N:31585,75,0,,,461483337,426648920,419516035,514521907,31585,SRX12200566,SRS10176855,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94209,,0.09014,,0.74468,,0.50897,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66121,SRR15910176,SRX12200565,SRS10176860,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC2,LC2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_2_S2_R1_001.fastq.gz,fastq,2450938234.0,32590616.0,control 2 S2 R1 001.fastq.gz,0:75.20 1:0,A:628466312;C:568472904;G:556931536;T:689980376;N:7087106,75,0,,,628466312,568472904,556931536,689980376,7087106,SRX12200565,SRS10176860,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93782,,0.10577,,0.73699,,0.52,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66122,SRR15910177,SRX12200564,SRS10176856,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC1,LC1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_1_S1_R1_001.fastq.gz,fastq,2784628452.0,37009570.0,control 1 S1 R1 001.fastq.gz,0:75.24 1:0,A:700349148;C:654385631;G:634389883;T:789658848;N:5844942,75,0,,,700349148,654385631,634389883,789658848,5844942,SRX12200564,SRS10176856,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.9386,,0.08609,,0.73829,,0.53049,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 68333,SRR17714676,SRX13877733,SRS11747906,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,KO in 4,,strain:AB type|isolate:knockout|breed:KO in 4|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,KO in 4,KO in 4,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_KO-in-4_good_1.fq.gz Daniorerio_KO-in-4_good_2.fq.gz,fastq fastq,8086373448.0,27114595.0,Daniorerio KO in 4 good 1.fq.gz,0:149.11 1:149.11,A:2114635166;C:1908498639;G:1940373806;T:2122462453;N:403384,149,149,,,2114635166,1908498639,1940373806,2122462453,403384,SRX13877733,SRS11747906,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.93003,0.92992,0.06176,0.06188,0.70163,0.70429,0.48922,0.48903,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68334,SRR17714677,SRX13877732,SRS11747905,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,KO in 3,,strain:AB type|isolate:knockout|breed:KO in 3|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,KO in 3,KO in 3,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_KO-in-3_good_1.fq.gz Daniorerio_KO-in-3_good_2.fq.gz,fastq fastq,7347019970.0,24569878.0,Daniorerio KO in 3 good 1.fq.gz,0:149.51 1:149.51,A:1946037694;C:1706069195;G:1743974411;T:1950573440;N:365230,149,149,,,1946037694,1706069195,1743974411,1950573440,365230,SRX13877732,SRS11747905,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92803,0.92694,0.07821,0.07735,0.73367,0.73438,0.51472,0.52472,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68335,SRR17714678,SRX13877731,SRS11747903,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,KO in 2,,strain:AB type|isolate:knockout|breed:KO in 2|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,KO in 2,KO in 2,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_KO-in-2_good_1.fq.gz Daniorerio_KO-in-2_good_2.fq.gz,fastq fastq,8301032318.0,27790678.0,Daniorerio KO in 2 good 1.fq.gz,0:149.35 1:149.35,A:2179637699;C:1945190004;G:1982782844;T:2193010761;N:411010,149,149,,,2179637699,1945190004,1982782844,2193010761,411010,SRX13877731,SRS11747903,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.94456,0.9438,0.04849,0.04881,0.77191,0.77333,0.36067,0.3652,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68344,SRR17714687,SRX13877722,SRS11747895,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,KO in 1,,strain:AB type|isolate:knockout|breed:KO in 1|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,KO in 1,KO in 1,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_KO-in-1_good_1.fq.gz Daniorerio_KO-in-1_good_2.fq.gz,fastq fastq,7827900066.0,26180712.0,Daniorerio KO in 1 good 1.fq.gz,0:149.50 1:149.50,A:2080601651;C:1809785147;G:1846482300;T:2090643065;N:387903,149,149,,,2080601651,1809785147,1846482300,2090643065,387903,SRX13877722,SRS11747895,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92406,0.92332,0.08136,0.08064,0.72596,0.72541,0.51544,0.52064,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68345,SRR17714688,SRX13877721,SRS11747893,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,WT in 7,,strain:AB type|isolate:wild type|breed:WT in 7|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,WT in 7,WT in 7,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_WT-in-7_good_1.fq.gz Daniorerio_WT-in-7_good_2.fq.gz,fastq fastq,6525605248.0,21813313.0,Daniorerio WT in 7 good 1.fq.gz,0:149.58 1:149.58,A:1779067091;C:1469759022;G:1500273479;T:1776177556;N:328100,149,149,,,1779067091,1469759022,1500273479,1776177556,328100,SRX13877721,SRS11747893,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.90976,0.90971,0.08435,0.08468,0.72709,0.7289,0.5376,0.53733,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68346,SRR17714689,SRX13877720,SRS11747894,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,WT in 4,,strain:AB type|isolate:wild type|breed:WT in 4|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,WT in 4,WT in 4,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_WT-in-4_good_1.fq.gz Daniorerio_WT-in-4_good_2.fq.gz,fastq fastq,9307471244.0,31204763.0,Daniorerio WT in 4 good 1.fq.gz,0:149.14 1:149.14,A:2485339643;C:2146056187;G:2184301247;T:2491310160;N:464007,149,149,,,2485339643,2146056187,2184301247,2491310160,464007,SRX13877720,SRS11747894,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.93125,0.9303,0.08758,0.08737,0.72042,0.72066,0.50621,0.50241,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68347,SRR17714690,SRX13877719,SRS11747892,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,WT in 2,,strain:AB type|isolate:wild type|breed:WT in 2|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,WT in 2,WT in 2,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_WT-in-2_good_1.fq.gz Daniorerio_WT-in-2_good_2.fq.gz,fastq fastq,7284861540.0,24415394.0,Daniorerio WT in 2 good 1.fq.gz,0:149.19 1:149.19,A:1956983508;C:1672191117;G:1698929394;T:1956391871;N:365650,149,149,,,1956983508,1672191117,1698929394,1956391871,365650,SRX13877719,SRS11747892,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92731,0.92546,0.09355,0.09268,0.73125,0.7317,0.53578,0.51601,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68348,SRR17714691,SRX13877718,SRS11747890,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,WT in 1,,strain:AB type|isolate:wild type|breed:WT in 1|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,WT in 1,WT in 1,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_WT-in-1_good_1.fq.gz Daniorerio_WT-in-1_good_2.fq.gz,fastq fastq,6846412620.0,22924105.0,Daniorerio WT in 1 good 1.fq.gz,0:149.33 1:149.33,A:1885463202;C:1523755204;G:1559518207;T:1877335306;N:340701,149,149,,,1885463202,1523755204,1559518207,1877335306,340701,SRX13877718,SRS11747890,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92598,0.92418,0.11749,0.1172,0.7124,0.71287,0.52007,0.52208,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68362,SRR17714705,SRX13877704,SRS11747876,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,MU in 4,,strain:AB type|isolate:point mutation|breed:MU in 4|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,MU in 4,MU in 4,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_MU-in-4_good_1.fq.gz Daniorerio_MU-in-4_good_2.fq.gz,fastq fastq,6981292910.0,23353513.0,Daniorerio MU in 4 good 1.fq.gz,0:149.47 1:149.47,A:1918651893;C:1556487274;G:1587959315;T:1917844487;N:349941,149,149,,,1918651893,1556487274,1587959315,1917844487,349941,SRX13877704,SRS11747876,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.90619,0.90539,0.10392,0.10257,0.72581,0.7262,0.50279,0.51648,115,115,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68363,SRR17714706,SRX13877703,SRS11747877,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,MU in 3,,strain:AB type|isolate:point mutation|breed:MU in 3|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,MU in 3,MU in 3,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_MU-in-3_good_1.fq.gz Daniorerio_MU-in-3_good_2.fq.gz,fastq fastq,6927732356.0,23196580.0,Daniorerio MU in 3 good 1.fq.gz,0:149.33 1:149.33,A:1882979804;C:1564547441;G:1599806601;T:1880055787;N:342723,149,149,,,1882979804,1564547441,1599806601,1880055787,342723,SRX13877703,SRS11747877,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92662,0.92461,0.09213,0.09138,0.70325,0.70512,0.49198,0.48956,128,128,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68364,SRR17714707,SRX13877702,SRS11747875,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,MU in 2,,strain:AB type|isolate:point mutation|breed:MU in 2|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,MU in 2,MU in 2,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_MU-in-2_good_1.fq.gz Daniorerio_MU-in-2_good_2.fq.gz,fastq fastq,5935464130.0,19846848.0,Daniorerio MU in 2 good 1.fq.gz,0:149.53 1:149.53,A:1636475982;C:1319113428;G:1341190439;T:1638386007;N:298274,149,149,,,1636475982,1319113428,1341190439,1638386007,298274,SRX13877702,SRS11747875,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92398,0.92343,0.10356,0.10369,0.71875,0.71999,0.51404,0.52599,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 68365,SRR17714708,SRX13877701,SRS11747874,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,MU in 1,,strain:AB type|isolate:point mutation|breed:MU in 1|age:3 month|dev stage:NA|sex:NA|tissue:intestine|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,MU in 1,MU in 1,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_MU-in-1_good_1.fq.gz Daniorerio_MU-in-1_good_2.fq.gz,fastq fastq,6216034614.0,20807316.0,Daniorerio MU in 1 good 1.fq.gz,0:149.37 1:149.37,A:1699351968;C:1392852246;G:1422263891;T:1701253575;N:312934,149,149,,,1699351968,1392852246,1422263891,1701253575,312934,SRX13877701,SRS11747874,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92907,0.92796,0.08789,0.0879,0.73277,0.73415,0.53788,0.53553,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Gut,Digestive System 71924,SRR22163283,SRX18142185,SRS15643653,SRP405971,PRJNA896758,Anti inflammatory potential of alginates in the zebrafish model of intestinal inflammation,PRJNA896758,Other,In this study we investigated the impact of dietary alginates on the intestinal transcriptomic landscape of the zebrafish model of intestinal inflammation,,,,,CT5 1,,Treatment:Treatment 1|replicate:biological replicate 5|read:forward read|strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:1 year|dev stage:Adult|sex:male|tissue:Intestine|collected by:Saima Rehman|collection date:2022 03|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,S1 5 1,S1 5 1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP405971,,,S1_5_1.fq.gz S1_5_2.fq.gz,fastq fastq,9014027400.0,30046758.0,S1 5 1.fq.gz,0:150 1:150,A:2459911620;C:2065534063;G:2054316794;T:2434183063;N:81860,150,150,,,2459911620,2065534063,2054316794,2434183063,81860,SRX18142185,SRS15643653,SRA1532106,Nord University|Faculty of Bioscience and Aquaculture,Nord University,2,0.93772,0.93725,0.08092,0.08041,0.70832,0.70978,0.53684,0.53606,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2022-11-02,Adult,Adult,Gut,Digestive System 71925,SRR22163284,SRX18142184,SRS15643652,SRP405971,PRJNA896758,Anti inflammatory potential of alginates in the zebrafish model of intestinal inflammation,PRJNA896758,Other,In this study we investigated the impact of dietary alginates on the intestinal transcriptomic landscape of the zebrafish model of intestinal inflammation,,,,,CT4 1,,Treatment:Treatment 1|replicate:biological replicate 4|read:forward read|strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:1 year|dev stage:Adult|sex:male|tissue:Intestine|collected by:Saima Rehman|collection date:2022 03|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,S1 4 1,S1 4 1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP405971,,,S1_4_1.fq.gz S1_4_2.fq.gz,fastq fastq,6553685700.0,21845619.0,S1 4 1.fq.gz,0:150 1:150,A:1784449261;C:1498869080;G:1499429078;T:1770878702;N:59579,150,150,,,1784449261,1498869080,1499429078,1770878702,59579,SRX18142184,SRS15643652,SRA1532106,Nord University|Faculty of Bioscience and Aquaculture,Nord University,2,0.93877,0.93743,0.08244,0.08155,0.739,0.74097,0.52274,0.52625,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2022-11-02,Adult,Adult,Gut,Digestive System 71926,SRR22163285,SRX18142183,SRS15643651,SRP405971,PRJNA896758,Anti inflammatory potential of alginates in the zebrafish model of intestinal inflammation,PRJNA896758,Other,In this study we investigated the impact of dietary alginates on the intestinal transcriptomic landscape of the zebrafish model of intestinal inflammation,,,,,AH4 1,,Treatment:Treatment 5|replicate:biological replicate 4|read:forward read|strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:1 year|dev stage:Adult|sex:male|tissue:Intestine|collected by:Saima Rehman|collection date:2022 03|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,S5 4 1,S5 4 1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP405971,,,S5_4_1.fq.gz S5_4_2.fq.gz,fastq fastq,7564526100.0,25215087.0,S5 4 1.fq.gz,0:150 1:150,A:2043866713;C:1743444091;G:1745247877;T:2031898136;N:69283,150,150,,,2043866713,1743444091,1745247877,2031898136,69283,SRX18142183,SRS15643651,SRA1532106,Nord University|Faculty of Bioscience and Aquaculture,Nord University,2,0.94099,0.94529,0.0771,0.07756,0.73265,0.73257,0.52445,0.52332,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2022-11-02,Adult,Adult,Gut,Digestive System