rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 48941,SRR7535399,SRX4403259,SRS3557748,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample43,F3HFL6,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3HFL6 L43,F3HFL6 L43,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3HFL6.fastq.gz,fastq,3513906206.0,41769622.0,F3HFL6.fastq.gz,0:84.13 1:0,A:977114573;C:787720805;G:749651347;T:999364818;N:54663,84,0,,,977114573,787720805,749651347,999364818,54663,SRX4403259,SRS3557748,SRA741696,Gov of Canada,Gov of Canada,1,0.95671,,0.06506,,0.78228,,0.56305,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48942,SRR7535400,SRX4403258,SRS3557747,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample41,F3HFL4,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3HFL4 L41,F3HFL4 L41,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3HFL4.fastq.gz,fastq,1925799513.0,22885356.0,F3HFL4.fastq.gz,0:84.15 1:0,A:524298249;C:430610288;G:398055851;T:572803437;N:31688,84,0,,,524298249,430610288,398055851,572803437,31688,SRX4403258,SRS3557747,SRA741696,Gov of Canada,Gov of Canada,1,0.93737,,0.10113,,0.81022,,0.55692,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48943,SRR7535401,SRX4403257,SRS3557746,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample42,F3HFL5,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3HFL5 L42,F3HFL5 L42,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3HFL5.fastq.gz,fastq,2861742645.0,33977477.0,F3HFL5.fastq.gz,0:84.22 1:0,A:771306767;C:644570838;G:600324085;T:845486554;N:54401,84,0,,,771306767,644570838,600324085,845486554,54401,SRX4403257,SRS3557746,SRA741696,Gov of Canada,Gov of Canada,1,0.95145,,0.08188,,0.78084,,0.53906,,82,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-07-18,Adult,Adult,Kidney,Renal System 48944,SRR7535402,SRX4403256,SRS3557745,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample21,F0HFL8,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0HFL8 L21,F0HFL8 L21,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0HFL8.fastq.gz,fastq,3270195567.0,43558704.0,F0HFL8.fastq.gz,0:75.08 1:0,A:931015183;C:710121529;G:674934313;T:954020799;N:103743,75,0,,,931015183,710121529,674934313,954020799,103743,SRX4403256,SRS3557745,SRA741696,Gov of Canada,Gov of Canada,1,0.94496,,0.07696,,0.74931,,0.55469,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48945,SRR7535403,SRX4403255,SRS3557744,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample22,F3CTR1,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3CTR1 L22,F3CTR1 L22,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3CTR1.fastq.gz,fastq,2224836049.0,29606943.0,F3CTR1.fastq.gz,0:75.15 1:0,A:610515696;C:506550058;G:471139873;T:636560130;N:70292,75,0,,,610515696,506550058,471139873,636560130,70292,SRX4403255,SRS3557744,SRA741696,Gov of Canada,Gov of Canada,1,0.95447,,0.0754,,0.78066,,0.54856,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48946,SRR7535404,SRX4403254,SRS3557743,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample23,F3CTR2,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3CTR2 L23,F3CTR2 L23,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3CTR2.fastq.gz,fastq,3106167820.0,36871227.0,F3CTR2.fastq.gz,0:84.24 1:0,A:844192333;C:713285812;G:657110592;T:891518698;N:60385,84,0,,,844192333,713285812,657110592,891518698,60385,SRX4403254,SRS3557743,SRA741696,Gov of Canada,Gov of Canada,1,0.9554,,0.06997,,0.79275,,0.52557,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48947,SRR7535405,SRX4403253,SRS3557742,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample24,F3CTR3,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3CTR3 L24,F3CTR3 L24,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3CTR3.fastq.gz,fastq,2619378393.0,31091747.0,F3CTR3.fastq.gz,0:84.25 1:0,A:699692748;C:600624113;G:564410765;T:754600796;N:49971,84,0,,,699692748,600624113,564410765,754600796,49971,SRX4403253,SRS3557742,SRA741696,Gov of Canada,Gov of Canada,1,0.95974,,0.06076,,0.7932,,0.54096,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48948,SRR7535406,SRX4403252,SRS3557741,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample25,F3CTR4,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3CTR4 L25,F3CTR4 L25,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3CTR4.fastq.gz,fastq,3292260052.0,39078900.0,F3CTR4.fastq.gz,0:84.25 1:0,A:880017890;C:753064780;G:705989117;T:953125639;N:62626,84,0,,,880017890,753064780,705989117,953125639,62626,SRX4403252,SRS3557741,SRA741696,Gov of Canada,Gov of Canada,1,0.95321,,0.07242,,0.75666,,0.54587,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48949,SRR7535407,SRX4403251,SRS3557740,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample26,F3CTR5,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3CTR5 L26,F3CTR5 L26,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3CTR5.fastq.gz,fastq,3007976354.0,35696121.0,F3CTR5.fastq.gz,0:84.27 1:0,A:778578191;C:708292962;G:650565998;T:870481710;N:57493,84,0,,,778578191,708292962,650565998,870481710,57493,SRX4403251,SRS3557740,SRA741696,Gov of Canada,Gov of Canada,1,0.95344,,0.06722,,0.7597,,0.52143,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48950,SRR7535408,SRX4403250,SRS3557739,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample27,F3CTR6,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3CTR6 L27,F3CTR6 L27,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3CTR6.fastq.gz,fastq,2550867627.0,31161224.0,F3CTR6.fastq.gz,0:81.86 1:0,A:1115959207;C:419324274;G:453781546;T:561761915;N:40685,81,0,,,1115959207,419324274,453781546,561761915,40685,SRX4403250,SRS3557739,SRA741696,Gov of Canada,Gov of Canada,1,0.92888,,0.24434,,0.77749,,0.51629,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48951,SRR7535409,SRX4403249,SRS3557738,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample28,F3CTR7,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3CTR7 L28,F3CTR7 L28,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3CTR7.fastq.gz,fastq,2059768786.0,24468469.0,F3CTR7.fastq.gz,0:84.18 1:0,A:558059300;C:468460643;G:436031484;T:597176496;N:40863,84,0,,,558059300,468460643,436031484,597176496,40863,SRX4403249,SRS3557738,SRA741696,Gov of Canada,Gov of Canada,1,0.94686,,0.07284,,0.77459,,0.54014,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48952,SRR7535410,SRX4403248,SRS3557737,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample29,F3CTR8,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3CTR8 L29,F3CTR8 L29,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3CTR8.fastq.gz,fastq,3053213414.0,36279614.0,F3CTR8.fastq.gz,0:84.16 1:0,A:815581740;C:713814496;G:673475187;T:850294355;N:47636,84,0,,,815581740,713814496,673475187,850294355,47636,SRX4403248,SRS3557737,SRA741696,Gov of Canada,Gov of Canada,1,0.9571,,0.06714,,0.75369,,0.54721,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48953,SRR7535411,SRX4403247,SRS3557736,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample30,F3LFL1,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3LFL1 L30,F3LFL1 L30,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3LFL1.fastq.gz,fastq,2028343411.0,26987895.0,F3LFL1.fastq.gz,0:75.16 1:0,A:548593649;C:466971074;G:433327481;T:579386722;N:64485,75,0,,,548593649,466971074,433327481,579386722,64485,SRX4403247,SRS3557736,SRA741696,Gov of Canada,Gov of Canada,1,0.95498,,0.0741,,0.7895,,0.5309,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-07-18,Adult,Adult,Kidney,Renal System 48954,SRR7535412,SRX4403246,SRS3557735,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample8,F0CTR8,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0CTR8 L8,F0CTR8 L8,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0CTR8.fastq.gz,fastq,2222889019.0,29574868.0,F0CTR8.fastq.gz,0:75.16 1:0,A:596733500;C:518690507;G:486721856;T:620675208;N:67948,75,0,,,596733500,518690507,486721856,620675208,67948,SRX4403246,SRS3557735,SRA741696,Gov of Canada,Gov of Canada,1,0.95446,,0.05545,,0.74815,,0.51889,,74,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48955,SRR7535413,SRX4403245,SRS3557734,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample7,F0CTR7,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0CTR7 L7,F0CTR7 L7,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0CTR7.fastq.gz,fastq,4544318503.0,53947186.0,F0CTR7.fastq.gz,0:84.24 1:0,A:1292030811;C:991354554;G:942494048;T:1318393334;N:45756,84,0,,,1292030811,991354554,942494048,1318393334,45756,SRX4403245,SRS3557734,SRA741696,Gov of Canada,Gov of Canada,1,0.9503,,0.06778,,0.75743,,0.55348,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-07-18,Adult,Adult,Kidney,Renal System 48956,SRR7535414,SRX4403244,SRS3557733,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample6,F0CTR6,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0CTR6 L6,F0CTR6 L6,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0CTR6.fastq.gz,fastq,2358891923.0,31391321.0,F0CTR6.fastq.gz,0:75.14 1:0,A:685288644;C:509459791;G:472389125;T:691678243;N:76120,75,0,,,685288644,509459791,472389125,691678243,76120,SRX4403244,SRS3557733,SRA741696,Gov of Canada,Gov of Canada,1,0.94166,,0.07795,,0.76195,,0.53942,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48957,SRR7535415,SRX4403243,SRS3557732,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample5,F0CTR5,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0CTR5 L5,F0CTR5 L5,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0CTR5.fastq.gz,fastq,2588676344.0,30755117.0,F0CTR5.fastq.gz,0:84.17 1:0,A:720655179;C:582123937;G:551878210;T:733977893;N:41125,84,0,,,720655179,582123937,551878210,733977893,41125,SRX4403243,SRS3557732,SRA741696,Gov of Canada,Gov of Canada,1,0.95586,,0.06561,,0.7808,,0.54698,,83,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48958,SRR7535416,SRX4403242,SRS3557731,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample4,F0CTR4,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0CTR4 L4,F0CTR4 L4,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0CTR4.fastq.gz,fastq,2959885275.0,35175450.0,F0CTR4.fastq.gz,0:84.15 1:0,A:799876836;C:675392846;G:616854860;T:867718008;N:42725,84,0,,,799876836,675392846,616854860,867718008,42725,SRX4403242,SRS3557731,SRA741696,Gov of Canada,Gov of Canada,1,0.93974,,0.07556,,0.76238,,0.50131,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48959,SRR7535417,SRX4403241,SRS3557730,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample3,F0CTR3,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0CTR3 L3,F0CTR3 L3,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0CTR3.fastq.gz,fastq,3127991402.0,37019173.0,F0CTR3.fastq.gz,0:84.50 1:0,A:886233117;C:699410395;G:659031655;T:883271353;N:44882,84,0,,,886233117,699410395,659031655,883271353,44882,SRX4403241,SRS3557730,SRA741696,Gov of Canada,Gov of Canada,1,0.95481,,0.06332,,0.77818,,0.54263,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48960,SRR7535418,SRX4403240,SRS3557729,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample2,F0CTR2,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0CTR2 L2,F0CTR2 L2,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0CTR2.fastq.gz,fastq,4589246236.0,54480219.0,F0CTR2.fastq.gz,0:84.24 1:0,A:1318726005;C:987643623;G:940290206;T:1342543647;N:42755,84,0,,,1318726005,987643623,940290206,1342543647,42755,SRX4403240,SRS3557729,SRA741696,Gov of Canada,Gov of Canada,1,0.94686,,0.06888,,0.76278,,0.55045,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48961,SRR7535419,SRX4403239,SRS3557728,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample1,F0CTR1,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0CTR1 L1,F0CTR1 L1,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0CTR1.fastq.gz,fastq,2895973720.0,34407735.0,F0CTR1.fastq.gz,0:84.17 1:0,A:805217942;C:647195655;G:615425828;T:828086852;N:47443,84,0,,,805217942,647195655,615425828,828086852,47443,SRX4403239,SRS3557728,SRA741696,Gov of Canada,Gov of Canada,1,0.95771,,0.05823,,0.75475,,0.54018,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-07-18,Adult,Adult,Kidney,Renal System 48962,SRR7535420,SRX4403238,SRS3557727,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample10,F0LFL2,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0LFL2 L10,F0LFL2 L10,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0LFL2.fastq.gz,fastq,2221964224.0,29564609.0,F0LFL2.fastq.gz,0:75.16 1:0,A:622205890;C:491850489;G:469917376;T:637922036;N:68433,75,0,,,622205890,491850489,469917376,637922036,68433,SRX4403238,SRS3557727,SRA741696,Gov of Canada,Gov of Canada,1,0.95207,,0.06132,,0.76404,,0.5374,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48963,SRR7535421,SRX4403237,SRS3557726,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample9,F0LFL1,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0LFL1 L9,F0LFL1 L9,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0LFL1.fastq.gz,fastq,3222133895.0,38154313.0,F0LFL1.fastq.gz,0:84.45 1:0,A:920033678;C:715675242;G:671297913;T:915080329;N:46733,84,0,,,920033678,715675242,671297913,915080329,46733,SRX4403237,SRS3557726,SRA741696,Gov of Canada,Gov of Canada,1,0.95496,,0.07355,,0.76644,,0.54508,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48964,SRR7535422,SRX4403236,SRS3557725,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample20,F0HFL7,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0HFL7 L20,F0HFL7 L20,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0HFL7.fastq.gz,fastq,2800528980.0,33270152.0,F0HFL7.fastq.gz,0:84.18 1:0,A:745732773;C:657771159;G:624594638;T:772385369;N:45041,84,0,,,745732773,657771159,624594638,772385369,45041,SRX4403236,SRS3557725,SRA741696,Gov of Canada,Gov of Canada,1,0.9603,,0.05557,,0.75262,,0.53229,,83,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48965,SRR7535423,SRX4403235,SRS3557724,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample19,F0HFL6,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0HFL6 L19,F0HFL6 L19,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0HFL6.fastq.gz,fastq,3859582889.0,45819776.0,F0HFL6.fastq.gz,0:84.23 1:0,A:1042286597;C:895964648;G:843235146;T:1078059877;N:36621,84,0,,,1042286597,895964648,843235146,1078059877,36621,SRX4403235,SRS3557724,SRA741696,Gov of Canada,Gov of Canada,1,0.95288,,0.06929,,0.74,,0.52268,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48966,SRR7535424,SRX4403234,SRS3557723,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample16,F0HFL3,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0HFL3 L16,F0HFL3 L16,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0HFL3.fastq.gz,fastq,3793212903.0,45071794.0,F0HFL3.fastq.gz,0:84.16 1:0,A:1056360853;C:843514256;G:810627279;T:1082649387;N:61128,84,0,,,1056360853,843514256,810627279,1082649387,61128,SRX4403234,SRS3557723,SRA741696,Gov of Canada,Gov of Canada,1,0.95323,,0.06159,,0.76195,,0.54295,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48967,SRR7535425,SRX4403233,SRS3557722,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample15,F0HFL2,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0HFL2 L15,F0HFL2 L15,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0HFL2.fastq.gz,fastq,1653605409.0,19670232.0,F0HFL2.fastq.gz,0:84.07 1:0,A:482659933;C:340033054;G:332925206;T:497960425;N:26791,84,0,,,482659933,340033054,332925206,497960425,26791,SRX4403233,SRS3557722,SRA741696,Gov of Canada,Gov of Canada,1,0.94378,,0.07818,,0.76654,,0.56019,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48968,SRR7535426,SRX4403232,SRS3557721,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample18,F0HFL5,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0HFL5 L18,F0HFL5 L18,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0HFL5.fastq.gz,fastq,2841372173.0,33797215.0,F0HFL5.fastq.gz,0:84.07 1:0,A:778391982;C:632117929;G:585902045;T:844912619;N:47598,84,0,,,778391982,632117929,585902045,844912619,47598,SRX4403232,SRS3557721,SRA741696,Gov of Canada,Gov of Canada,1,0.93559,,0.07561,,0.76895,,0.55817,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48969,SRR7535427,SRX4403231,SRS3557719,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample17,F0HFL4,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0HFL4 L17,F0HFL4 L17,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0HFL4.fastq.gz,fastq,3160174261.0,37565835.0,F0HFL4.fastq.gz,0:84.12 1:0,A:884296991;C:697282059;G:667815635;T:910728716;N:50860,84,0,,,884296991,697282059,667815635,910728716,50860,SRX4403231,SRS3557719,SRA741696,Gov of Canada,Gov of Canada,1,0.95258,,0.06896,,0.75753,,0.53648,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48970,SRR7535428,SRX4403230,SRS3557720,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample12,F0LFL4,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0LFL4 L12,F0LFL4 L12,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0LFL4.fastq.gz,fastq,2872309444.0,34017640.0,F0LFL4.fastq.gz,0:84.44 1:0,A:838123566;C:615148814;G:580099793;T:838897200;N:40071,84,0,,,838123566,615148814,580099793,838897200,40071,SRX4403230,SRS3557720,SRA741696,Gov of Canada,Gov of Canada,1,0.94655,,0.07484,,0.76055,,0.53193,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48971,SRR7535429,SRX4403229,SRS3557718,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample11,F0LFL3,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0LFL3 L11,F0LFL3 L11,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0LFL3.fastq.gz,fastq,4819656374.0,57208863.0,F0LFL3.fastq.gz,0:84.25 1:0,A:1344664833;C:1065935819;G:1021114550;T:1387895714;N:45458,84,0,,,1344664833,1065935819,1021114550,1387895714,45458,SRX4403229,SRS3557718,SRA741696,Gov of Canada,Gov of Canada,1,0.94968,,0.06704,,0.75063,,0.53941,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48972,SRR7535430,SRX4403228,SRS3557717,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample14,F0HFL1,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0HFL1 L14,F0HFL1 L14,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0HFL1.fastq.gz,fastq,4382160649.0,58413517.0,F0HFL1.fastq.gz,0:75.02 1:0,A:1369437301;C:861646512;G:809593345;T:1341341422;N:142069,75,0,,,1369437301,861646512,809593345,1341341422,142069,SRX4403228,SRS3557717,SRA741696,Gov of Canada,Gov of Canada,1,0.9466,,0.08719,,0.7794,,0.54372,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-07-18,Adult,Adult,Kidney,Renal System 48973,SRR7535431,SRX4403227,SRS3557716,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample13,F0LFL5,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F0LFL5 L13,F0LFL5 L13,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F0LFL5.fastq.gz,fastq,4135324561.0,55077889.0,F0LFL5.fastq.gz,0:75.08 1:0,A:1152758595;C:923324532;G:883060803;T:1176048511;N:132120,75,0,,,1152758595,923324532,883060803,1176048511,132120,SRX4403227,SRS3557716,SRA741696,Gov of Canada,Gov of Canada,1,0.94936,,0.07427,,0.7385,,0.54293,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48974,SRR7535432,SRX4403226,SRS3557715,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample38,F3HFL1,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3HFL1 L38,F3HFL1 L38,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3HFL1.fastq.gz,fastq,4205002265.0,49802474.0,F3HFL1.fastq.gz,0:84.43 1:0,A:1296210304;C:849397168;G:835453941;T:1223875076;N:65776,84,0,,,1296210304,849397168,835453941,1223875076,65776,SRX4403226,SRS3557715,SRA741696,Gov of Canada,Gov of Canada,1,0.95559,,0.0917,,0.78044,,0.61361,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48975,SRR7535433,SRX4403225,SRS3557714,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample37,F3LFL8,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3LFL8 L37,F3LFL8 L37,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3LFL8.fastq.gz,fastq,2259705259.0,26829092.0,F3LFL8.fastq.gz,0:84.23 1:0,A:609862793;C:515167321;G:475663194;T:658969872;N:42079,84,0,,,609862793,515167321,475663194,658969872,42079,SRX4403225,SRS3557714,SRA741696,Gov of Canada,Gov of Canada,1,0.94002,,0.10434,,0.75369,,0.51155,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48976,SRR7535434,SRX4403224,SRS3557713,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample36,F3LFL7,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3LFL7 L36,F3LFL7 L36,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3LFL7.fastq.gz,fastq,2341138815.0,27781759.0,F3LFL7.fastq.gz,0:84.27 1:0,A:608466569;C:545924123;G:501493228;T:685210306;N:44589,84,0,,,608466569,545924123,501493228,685210306,44589,SRX4403224,SRS3557713,SRA741696,Gov of Canada,Gov of Canada,1,0.94523,,0.08263,,0.75284,,0.53752,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48977,SRR7535435,SRX4403223,SRS3557712,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample35,F3LFL6,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3LFL6 L35,F3LFL6 L35,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3LFL6.fastq.gz,fastq,2937598885.0,34879736.0,F3LFL6.fastq.gz,0:84.22 1:0,A:782454526;C:678806792;G:617210082;T:859072883;N:54602,84,0,,,782454526,678806792,617210082,859072883,54602,SRX4403223,SRS3557712,SRA741696,Gov of Canada,Gov of Canada,1,0.94207,,0.08495,,0.74897,,0.5069,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48978,SRR7535436,SRX4403222,SRS3557711,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample34,F3LFL5,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3LFL5 L34,F3LFL5 L34,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3LFL5.fastq.gz,fastq,2497066825.0,29658022.0,F3LFL5.fastq.gz,0:84.20 1:0,A:694874960;C:555774576;G:516525097;T:729844057;N:48135,84,0,,,694874960,555774576,516525097,729844057,48135,SRX4403222,SRS3557711,SRA741696,Gov of Canada,Gov of Canada,1,0.94238,,0.09347,,0.76404,,0.51048,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48979,SRR7535437,SRX4403221,SRS3557710,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample33,F3LFL4,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3LFL4 L33,F3LFL4 L33,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3LFL4.fastq.gz,fastq,3167171318.0,37581804.0,F3LFL4.fastq.gz,0:84.27 1:0,A:836474638;C:736638892;G:687417511;T:906580806;N:59471,84,0,,,836474638,736638892,687417511,906580806,59471,SRX4403221,SRS3557710,SRA741696,Gov of Canada,Gov of Canada,1,0.95381,,0.0776,,0.74432,,0.49786,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48980,SRR7535438,SRX4403220,SRS3557709,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample32,F3LFL3,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3LFL3 L32,F3LFL3 L32,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3LFL3.fastq.gz,fastq,3969872568.0,47103172.0,F3LFL3.fastq.gz,0:84.28 1:0,A:1087899347;C:891968649;G:846315646;T:1143611603;N:77323,84,0,,,1087899347,891968649,846315646,1143611603,77323,SRX4403220,SRS3557709,SRA741696,Gov of Canada,Gov of Canada,1,0.94717,,0.08461,,0.74714,,0.53355,,84,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48981,SRR7535439,SRX4403219,SRS3557708,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample31,F3LFL2,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3LFL2 L31,F3LFL2 L31,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3LFL2.fastq.gz,fastq,2401613544.0,31959346.0,F3LFL2.fastq.gz,0:75.15 1:0,A:673230842;C:536445759;G:492980009;T:698882870;N:74064,75,0,,,673230842,536445759,492980009,698882870,74064,SRX4403219,SRS3557708,SRA741696,Gov of Canada,Gov of Canada,1,0.95198,,0.08486,,0.7878,,0.55398,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48982,SRR7535440,SRX4403218,SRS3557706,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample40,F3HFL3,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3HFL3 L40,F3HFL3 L40,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3HFL3.fastq.gz,fastq,2266960447.0,26913177.0,F3HFL3.fastq.gz,0:84.23 1:0,A:590021990;C:520283209;G:479594101;T:677024566;N:36581,84,0,,,590021990,520283209,479594101,677024566,36581,SRX4403218,SRS3557706,SRA741696,Gov of Canada,Gov of Canada,1,0.94897,,0.08251,,0.80848,,0.57355,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 48983,SRR7535441,SRX4403217,SRS3557707,SRP154295,PRJNA481502,Transgenerational hypocortisolism and behavioral disruption is induced by the antidepressant fluoxetine in male zebrafish Danio rerio,PRJNA481502,Transcriptome Analysis,Transcriptomic profiling of the whole kidney revealed that early fluoxetine exposure significantly transgenerationally modified numerous pathways closely associated with cortisol synthesis in the male adults from the F0 and F3 generations.,,pubmed:30530669,,sample39,F3HFL2,,strain:AB strain|age:6 mpf organism or animal,,,,,,,,,RNA seq of Danio rerio: male kidney,F3HFL2 L39,F3HFL2 L39,mRNA seq from total RNA on Illumina Neoprep,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,SRP154295,,,F3HFL2.fastq.gz,fastq,2749128323.0,32649931.0,F3HFL2.fastq.gz,0:84.20 1:0,A:739282939;C:602311637;G:590565979;T:816929591;N:38177,84,0,,,739282939,602311637,590565979,816929591,38177,SRX4403217,SRS3557707,SRA741696,Gov of Canada,Gov of Canada,1,0.9613,,0.09046,,0.8104,,0.62158,,85,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Canada,2018-12-11,Adult,Adult,Kidney,Renal System 68331,SRR17714674,SRX13877735,SRS11747907,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,KO ki 2,,strain:AB type|isolate:knockout|breed:KO ki 2|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,KO ki 2,KO ki 2,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_KO-ki-2_good_1.fq.gz Daniorerio_KO-ki-2_good_2.fq.gz,fastq fastq,6459301738.0,21580856.0,Daniorerio KO ki 2 good 1.fq.gz,0:149.65 1:149.65,A:1784875806;C:1432248891;G:1473557552;T:1768292520;N:326969,149,149,,,1784875806,1432248891,1473557552,1768292520,326969,SRX13877735,SRS11747907,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92356,0.92283,0.11927,0.11877,0.69116,0.69286,0.51648,0.51904,122,122,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68332,SRR17714675,SRX13877734,SRS11747908,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,KO ki 1,,strain:AB type|isolate:knockout|breed:KO ki 1|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,KO ki 1,KO ki 1,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_KO-ki-1_good_1.fq.gz Daniorerio_KO-ki-1_good_2.fq.gz,fastq fastq,6099339068.0,20376162.0,Daniorerio KO ki 1 good 1.fq.gz,0:149.67 1:149.67,A:1680007435;C:1355561431;G:1393826814;T:1669638827;N:304561,149,149,,,1680007435,1355561431,1393826814,1669638827,304561,SRX13877734,SRS11747908,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92276,0.92011,0.1239,0.12427,0.69353,0.69457,0.52537,0.52715,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68340,SRR17714683,SRX13877726,SRS11747899,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,WT ki 7,,strain:AB type|isolate:wild type|breed:WT ki 7|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,WT ki 7,WT ki 7,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_WT-ki-7_good_1.fq.gz Daniorerio_WT-ki-7_good_2.fq.gz,fastq fastq,5728682916.0,19186928.0,Daniorerio WT ki 7 good 1.fq.gz,0:149.29 1:149.29,A:1579153464;C:1273656952;G:1312180039;T:1563403341;N:289120,149,149,,,1579153464,1273656952,1312180039,1563403341,289120,SRX13877726,SRS11747899,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.93765,0.9349,0.10683,0.10543,0.71248,0.71313,0.48682,0.50333,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68341,SRR17714684,SRX13877725,SRS11747898,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,WT ki 4,,strain:AB type|isolate:wild type|breed:WT ki 4|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,WT ki 4,WT ki 4,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_WT-ki-4_good_1.fq.gz Daniorerio_WT-ki-4_good_2.fq.gz,fastq fastq,6538230102.0,21840569.0,Daniorerio WT ki 4 good 1.fq.gz,0:149.68 1:149.68,A:1712166805;C:1543306905;G:1570047290;T:1712688420;N:20682,149,149,,,1712166805,1543306905,1570047290,1712688420,20682,SRX13877725,SRS11747898,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.93239,0.93289,0.03511,0.03508,0.71285,0.71313,0.4785,0.48034,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68342,SRR17714685,SRX13877724,SRS11747897,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,WT ki 3,,strain:AB type|isolate:wild type|breed:WT ki 3|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,WT ki 3,WT ki 3,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_WT-ki-3_good_1.fq.gz Daniorerio_WT-ki-3_good_2.fq.gz,fastq fastq,6535179260.0,21823644.0,Daniorerio WT ki 3 good 1.fq.gz,0:149.73 1:149.73,A:1783453491;C:1473349126;G:1511499971;T:1766854951;N:21721,149,149,,,1783453491,1473349126,1511499971,1766854951,21721,SRX13877724,SRS11747897,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.93369,0.93255,0.097,0.09643,0.67848,0.6788,0.51146,0.50793,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68343,SRR17714686,SRX13877723,SRS11747896,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,WT ki 2,,strain:AB type|isolate:wild type|breed:WT ki 2|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,WT ki 2,WT ki 2,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_WT-ki-2_good_1.fq.gz Daniorerio_WT-ki-2_good_2.fq.gz,fastq fastq,7459816636.0,24952918.0,Daniorerio WT ki 2 good 1.fq.gz,0:149.48 1:149.48,A:2038726076;C:1677988595;G:1720392114;T:2022686081;N:23770,149,149,,,2038726076,1677988595,1720392114,2022686081,23770,SRX13877723,SRS11747896,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92812,0.92786,0.12105,0.12019,0.69347,0.69463,0.51823,0.51342,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68358,SRR17714701,SRX13877708,SRS11747880,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,MU ki 4,,strain:AB type|isolate:point mutation|breed:MU ki 4|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,MU ki 4,MU ki 4,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_MU-ki-4_good_1.fq.gz Daniorerio_MU-ki-4_good_2.fq.gz,fastq fastq,6642468464.0,22227277.0,Daniorerio MU ki 4 good 1.fq.gz,0:149.42 1:149.42,A:1806510861;C:1499915463;G:1544497199;T:1791210925;N:334016,149,149,,,1806510861,1499915463,1544497199,1791210925,334016,SRX13877708,SRS11747880,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.93502,0.93305,0.0993,0.09919,0.68519,0.68617,0.50471,0.502,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68359,SRR17714702,SRX13877707,SRS11747881,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,MU ki 3,,strain:AB type|isolate:point mutation|breed:MU ki 3|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,MU ki 3,MU ki 3,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_MU-ki-3_good_1.fq.gz Daniorerio_MU-ki-3_good_2.fq.gz,fastq fastq,8129697250.0,27275691.0,Daniorerio MU ki 3 good 1.fq.gz,0:149.03 1:149.03,A:2186818686;C:1865874012;G:1906801031;T:2169800472;N:403049,149,149,,,2186818686,1865874012,1906801031,2169800472,403049,SRX13877707,SRS11747881,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.93373,0.93291,0.09256,0.09199,0.68363,0.68582,0.50121,0.49901,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68360,SRR17714703,SRX13877706,SRS11747879,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,MU ki 2,,strain:AB type|isolate:point mutation|breed:MU ki 2|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,MU ki 2,MU ki 2,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_MU-ki-2_good_1.fq.gz Daniorerio_MU-ki-2_good_2.fq.gz,fastq fastq,8613479966.0,28864538.0,Daniorerio MU ki 2 good 1.fq.gz,0:149.21 1:149.21,A:2341268944;C:1952093439;G:1997306668;T:2322383690;N:427225,149,149,,,2341268944,1952093439,1997306668,2322383690,427225,SRX13877706,SRS11747879,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92686,0.92514,0.10387,0.10349,0.68278,0.68465,0.5126,0.51299,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68361,SRR17714704,SRX13877705,SRS11747878,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,MU ki 1,,strain:AB type|isolate:point mutation|breed:MU ki 1|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,MU ki 1,MU ki 1,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_MU-ki-1_good_1.fq.gz Daniorerio_MU-ki-1_good_2.fq.gz,fastq fastq,6361721340.0,21272731.0,Daniorerio MU ki 1 good 1.fq.gz,0:149.53 1:149.53,A:1764729780;C:1403968855;G:1444221125;T:1748483646;N:317934,149,149,,,1764729780,1403968855,1444221125,1748483646,317934,SRX13877705,SRS11747878,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.91983,0.91698,0.11213,0.11,0.69603,0.69816,0.51786,0.51691,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68375,SRR17714718,SRX13877691,SRS11747864,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,KO ki 4,,strain:AB type|isolate:knockout|breed:KO ki 4|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,KO ki 4,KO ki 4,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_KO-ki-4_good_1.fq.gz Daniorerio_KO-ki-4_good_2.fq.gz,fastq fastq,7271417184.0,24262326.0,Daniorerio KO ki 4 good 1.fq.gz,0:149.85 1:149.85,A:1942212311;C:1672440540;G:1729389358;T:1927007172;N:367803,149,149,,,1942212311,1672440540,1729389358,1927007172,367803,SRX13877691,SRS11747864,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.93084,0.92784,0.10122,0.10007,0.70496,0.70648,0.52353,0.51998,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68376,SRR17714719,SRX13877690,SRS11747863,SRP356388,PRJNA799842,RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.,PRJNA799842,Other,To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.,,,,,KO ki 3,,strain:AB type|isolate:knockout|breed:KO ki 3|age:3 month|dev stage:NA|sex:NA|tissue:kidney|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of zebrafish,KO ki 3,KO ki 3,RNA seq,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2500,,SRP356388,,loader:fastq load.py,Daniorerio_KO-ki-3_good_1.fq.gz Daniorerio_KO-ki-3_good_2.fq.gz,fastq fastq,7380278536.0,24738542.0,Daniorerio KO ki 3 good 1.fq.gz,0:149.17 1:149.17,A:1960997842;C:1716280740;G:1751732155;T:1950894353;N:373446,149,149,,,1960997842,1716280740,1751732155,1950894353,373446,SRX13877690,SRS11747863,SRA1360632,South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource,South China Sea Institute of Oceanology,2,0.92496,0.9235,0.12088,0.11977,0.69923,0.69968,0.53733,0.53696,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2022-01-27,Adult,Adult,Kidney,Renal System 68886,SRR18218068,SRX14364510,SRS12177805,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 1,,strain:CG1|age:not collected|sex:not collected|tissue:whole kidney marrow|birth date: |death date:16 07 2019|genotype:Wild type|biological replicate:WKM CG1 biological replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow CG1,WKM CG1 1,WKM CG1 1,CG1 WT zebrafish were sacrificed to harvest the whole kidney marrow WKM for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAAARAAPEI-P1A1_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAARAAPEI-P1A1_2.fq.gz,fastq fastq,18015161100.0,60050537.0,FCHT5GHDSXX L1 HKRDZEBmfpEAAARAAPEI P1A1 1.fq.gz,0:150 1:150,A:4626900295;C:4400273225;G:4421661364;T:4566300408;N:25808,150,150,,,4626900295,4400273225,4421661364,4566300408,25808,SRX14364510,SRS12177805,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.94715,0.94844,0.03897,0.03893,0.72176,0.72182,0.51658,0.50581,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Undetermined,Undetermined,Kidney,Renal System 68901,SRR18218080,SRX14364495,SRS12177790,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 P2mut 7,,strain:CG1|age:8 month|sex:not collected|tissue:whole kidney marrow|birth date:17 11 2018|death date:17 07 2019|genotype:rag2:hIL7R mut2 tdTomato|biological replicate:WKM CG1 P2mut biological replicate 6|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow stably expressing human mutant IL7R CG1,WKM CG1 P2mut 7,WKM CG1 P2mut 7,CG1 zebrafish stably expressing human mutant IL7R were sacrificed without xxx of overt leukemia and whole kidney marrow WKM was harvested for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAAKRAAPEI-P26C2_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAKRAAPEI-P26C2_2.fq.gz,fastq fastq,16762989900.0,55876633.0,FCHT5GHDSXX L1 HKRDZEBmfpEAAKRAAPEI P26C2 1.fq.gz,0:150 1:150,A:4442983154;C:3951385839;G:4007817362;T:4360778933;N:24612,150,150,,,4442983154,3951385839,4007817362,4360778933,24612,SRX14364495,SRS12177790,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.94363,0.94514,0.04876,0.04869,0.73369,0.73379,0.53344,0.5343,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Adult,Adult,Kidney,Renal System 68902,SRR18218081,SRX14364494,SRS12177789,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 P2mut 6,,strain:CG1|age:8 month|sex:not collected|tissue:whole kidney marrow|birth date:17 11 2018|death date:17 07 2019|genotype:rag2:hIL7R mut2 tdTomato|biological replicate:WKM CG1 P2mut biological replicate 5|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow stably expressing human mutant IL7R CG1,WKM CG1 P2mut 6,WKM CG1 P2mut 6,CG1 zebrafish stably expressing human mutant IL7R were sacrificed without xxx of overt leukemia and whole kidney marrow WKM was harvested for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAAJRABPEI-P14B2_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAJRABPEI-P14B2_2.fq.gz,fastq fastq,18194856000.0,60649520.0,FCHT5GHDSXX L1 HKRDZEBmfpEAAJRABPEI P14B2 1.fq.gz,0:150 1:150,A:4776137612;C:4328521118;G:4394651128;T:4695520357;N:25785,150,150,,,4776137612,4328521118,4394651128,4695520357,25785,SRX14364494,SRS12177789,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.94185,0.94348,0.05607,0.05578,0.73332,0.73281,0.5284,0.52266,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Adult,Adult,Kidney,Renal System 68903,SRR18218082,SRX14364493,SRS12177788,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 P2mut 5,,strain:CG1|age:8 month|sex:not collected|tissue:whole kidney marrow|birth date:17 11 2018|death date:17 07 2019|genotype:rag2:hIL7R mut2 tdTomato|biological replicate:WKM CG1 P2mut biological replicate 4|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow stably expressing human mutant IL7R CG1,WKM CG1 P2mut 5,WKM CG1 P2mut 5,CG1 zebrafish stably expressing human mutant IL7R were sacrificed without xxx of overt leukemia and whole kidney marrow WKM was harvested for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAAIRAAPEI-P2A2_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAIRAAPEI-P2A2_2.fq.gz,fastq fastq,18218380200.0,60727934.0,FCHT5GHDSXX L1 HKRDZEBmfpEAAIRAAPEI P2A2 1.fq.gz,0:150 1:150,A:4769839227;C:4349260666;G:4417289893;T:4681963719;N:26695,150,150,,,4769839227,4349260666,4417289893,4681963719,26695,SRX14364493,SRS12177788,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.94341,0.94473,0.04954,0.04988,0.71192,0.7121,0.52338,0.52876,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Adult,Adult,Kidney,Renal System 68904,SRR18218083,SRX14364492,SRS12177787,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 P2mut 4,,strain:CG1|age:8 month|sex:not collected|tissue:whole kidney marrow|birth date:17 11 2018|death date:17 07 2019|genotype:rag2:hIL7R mut2 tdTomato|biological replicate:WKM CG1 P2mut biological replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow stably expressing human mutant IL7R CG1,WKM CG1 P2mut 4,WKM CG1 P2mut 4,CG1 zebrafish stably expressing human mutant IL7R were sacrificed without xxx of overt leukemia and whole kidney marrow WKM was harvested for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAAHRABPEI-P85H1_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAHRABPEI-P85H1_2.fq.gz,fastq fastq,18205310400.0,60684368.0,FCHT5GHDSXX L1 HKRDZEBmfpEAAHRABPEI P85H1 1.fq.gz,0:150 1:150,A:4801894426;C:4309153266;G:4380582553;T:4713653706;N:26449,150,150,,,4801894426,4309153266,4380582553,4713653706,26449,SRX14364492,SRS12177787,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.94333,0.94441,0.04893,0.04829,0.73026,0.73101,0.52749,0.52823,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Adult,Adult,Kidney,Renal System 68905,SRR18218084,SRX14364491,SRS12177786,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 P2mut 3,,strain:CG1|age:8 month|sex:not collected|tissue:whole kidney marrow|birth date:17 11 2018|death date:17 07 2019|genotype:rag2:hIL7R mut2 tdTomato|biological replicate:WKM CG1 P2mut biological replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow stably expressing human mutant IL7R CG1,WKM CG1 P2mut 3,WKM CG1 P2mut 3,CG1 zebrafish stably expressing human mutant IL7R were sacrificed without xxx of overt leukemia and whole kidney marrow WKM was harvested for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAAGRAAPEI-P73G1_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAGRAAPEI-P73G1_2.fq.gz,fastq fastq,18143381100.0,60477937.0,FCHT5GHDSXX L1 HKRDZEBmfpEAAGRAAPEI P73G1 1.fq.gz,0:150 1:150,A:4669037373;C:4411451517;G:4473720090;T:4589145705;N:26415,150,150,,,4669037373,4411451517,4473720090,4589145705,26415,SRX14364491,SRS12177786,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.94105,0.94205,0.0448,0.04442,0.71553,0.71581,0.5171,0.51695,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Adult,Adult,Kidney,Renal System 68906,SRR18218085,SRX14364490,SRS12177785,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 5,,strain:CG1|age:not collected|sex:not collected|tissue:whole kidney marrow|birth date: |death date:16 07 2019|genotype:Wild type|biological replicate:WKM CG1 biological replicate 5|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow CG1,WKM CG1 5,WKM CG1 5,CG1 WT zebrafish were sacrificed to harvest the whole kidney marrow WKM for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAAERABPEI-P49E1_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAERABPEI-P49E1_2.fq.gz,fastq fastq,18184234500.0,60614115.0,FCHT5GHDSXX L1 HKRDZEBmfpEAAERABPEI P49E1 1.fq.gz,0:150 1:150,A:4903189861;C:4188361342;G:4259034739;T:4833623925;N:24633,150,150,,,4903189861,4188361342,4259034739,4833623925,24633,SRX14364490,SRS12177785,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.93501,0.93633,0.05446,0.05438,0.71583,0.71368,0.5862,0.56886,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Undetermined,Undetermined,Kidney,Renal System 68907,SRR18218087,SRX14364489,SRS12177784,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 4,,strain:CG1|age:not collected|sex:not collected|tissue:whole kidney marrow|birth date: |death date:16 07 2019|genotype:Wild type|biological replicate:WKM CG1 biological replicate 4|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow CG1,WKM CG1 4,WKM CG1 4,CG1 WT zebrafish were sacrificed to harvest the whole kidney marrow WKM for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAADRAAPEI-P37D1_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAADRAAPEI-P37D1_2.fq.gz,fastq fastq,18145210500.0,60484035.0,FCHT5GHDSXX L1 HKRDZEBmfpEAADRAAPEI P37D1 1.fq.gz,0:150 1:150,A:4734947807;C:4358845691;G:4370947434;T:4680443888;N:25680,150,150,,,4734947807,4358845691,4370947434,4680443888,25680,SRX14364489,SRS12177784,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.93212,0.93296,0.05289,0.05244,0.71433,0.71465,0.5085,0.49253,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Undetermined,Undetermined,Kidney,Renal System 68908,SRR18218088,SRX14364488,SRS12177783,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 3,,strain:CG1|age:not collected|sex:not collected|tissue:whole kidney marrow|birth date: |death date:16 07 2019|genotype:Wild type|biological replicate:WKM CG1 biological replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow CG1,WKM CG1 3,WKM CG1 3,CG1 WT zebrafish were sacrificed to harvest the whole kidney marrow WKM for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAACRAAPEI-P25C1_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAACRAAPEI-P25C1_2.fq.gz,fastq fastq,18002689500.0,60008965.0,FCHT5GHDSXX L1 HKRDZEBmfpEAACRAAPEI P25C1 1.fq.gz,0:150 1:150,A:4624819223;C:4407791489;G:4420933608;T:4549119250;N:25930,150,150,,,4624819223,4407791489,4420933608,4549119250,25930,SRX14364488,SRS12177783,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.94795,0.94827,0.03817,0.03816,0.72991,0.72987,0.49707,0.49202,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Undetermined,Undetermined,Kidney,Renal System 68909,SRR18218089,SRX14364487,SRS12177782,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 2,,strain:CG1|age:not collected|sex:not collected|tissue:whole kidney marrow|birth date: |death date:16 07 2019|genotype:Wild type|biological replicate:WKM CG1 biological replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow CG1,WKM CG1 2,WKM CG1 2,CG1 WT zebrafish were sacrificed to harvest the whole kidney marrow WKM for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAABRAAPEI-P13B1_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAABRAAPEI-P13B1_2.fq.gz,fastq fastq,18005451300.0,60018171.0,FCHT5GHDSXX L1 HKRDZEBmfpEAABRAAPEI P13B1 1.fq.gz,0:150 1:150,A:4630108478;C:4397001180;G:4413434449;T:4564880633;N:26560,150,150,,,4630108478,4397001180,4413434449,4564880633,26560,SRX14364487,SRS12177782,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.95098,0.95069,0.0373,0.03685,0.72312,0.72301,0.51708,0.51873,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Undetermined,Undetermined,Kidney,Renal System 68910,SRR18218086,SRX14364486,SRS12177781,SRP362416,PRJNA812715,Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia,PRJNA812715,Other,T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.,,,,,WKM CG1 P2mut 2,,strain:CG1|age:16 month|sex:not collected|tissue:whole kidney marrow|birth date:17 03 2018|death date:17 07 2019|genotype:rag2:hIL7R mut2 tdTomato|biological replicate:WKM CG1 P2mut biological replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA seq of Danio rerio whole kidney marrow stably expressing human mutant IL7R CG1,WKM CG1 P2mut 2,WKM CG1 P2mut 2,CG1 zebrafish stably expressing human mutant IL7R were sacrificed without xxx of overt leukemia and whole kidney marrow WKM was harvested for further analysis. The RNA was extracted from WKM cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP362416,,,FCHT5GHDSXX_L1_HKRDZEBmfpEAAFRAAPEI-P61F1_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAFRAAPEI-P61F1_2.fq.gz,fastq fastq,18210767700.0,60702559.0,FCHT5GHDSXX L1 HKRDZEBmfpEAAFRAAPEI P61F1 1.fq.gz,0:150 1:150,A:4648945418;C:4475968423;G:4497341113;T:4588486811;N:25935,150,150,,,4648945418,4475968423,4497341113,4588486811,25935,SRX14364486,SRS12177781,SRA1380603,Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab,Instituto de Medicina Molecular Joao Lobo Antunes,2,0.94563,0.94587,0.03836,0.03819,0.73148,0.73042,0.49281,0.49845,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Portugal,2022-03-04,Adult,Adult,Kidney,Renal System