rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
9356,ERR2983452,ERX2986068,ERS2955656,ERP112513,PRJEB30097,RNA Seq of Danionine species,E-MTAB-7476,Transcriptome Analysis,Tissues specific transcriptomes of Danio rerio Danio albolineatus and Danio aesculapii Brain Liver and Gonads.,ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05,,Protocols: Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers.,R3wB,SAMEA5147911,"University of Hamburg, Institute of Zoology, Molecular Animal Physiology",ENA FIRST PUBLIC:2018 12 05T17:02:27Z|ENA LAST UPDATE:2018 12 05T10:01:48Z|External Id:SAMEA5147911|INSDC center name:University of Hamburg Institute of Zoology Molecular Animal Physiology|INSDC first public:2018 12 05T17:02:27Z|INSDC last update:2018 12 05T10:01:48Z|INSDC status:public|Submitter Id:E MTAB 7476:R3wB|age:3|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|individual:3|organism part:brain|sample name:E MTAB 7476:R3wB|scientific name:Danio rerio|sex:female|strain:tu,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danio9 species,E MTAB 7476:R3wB p,R3wB p,RNA Seq of Danionine species,Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers.,Experimental Factor: organism:Danio rerio|Experimental Factor: organism part:brain,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina HiSeq 4000,2000FApplication ReadForward11RApplication ReadReverse101,ERP112513,Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danionine species,ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05,FCHNW2WBBXX_L6_HKZEBodsEAABRAAPEI-205_1.fq.gz FCHNW2WBBXX_L6_HKZEBodsEAABRAAPEI-205_2.fq.gz,fastq fastq,3622698000.0,18113490.0,E MTAB 7476:FCHNW2WBBXX L6 HKZEBodsEAABRAAPEI 205 ,0:100 1:100,A:988454110;C:825179148;G:816134465;T:992141776;N:788501,100,100,,,988454110,825179148,816134465,992141776,788501,ERX2986068,ERS2955656,ERA1674470,"University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive","University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive",2,0.9287,0.92856,0.14723,0.14721,0.6928,0.69469,0.4956,0.49616,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2018-12-05,Adult,Adult,Brain,Nervous System
9360,ERR2983448,ERX2986064,ERS2955652,ERP112513,PRJEB30097,RNA Seq of Danionine species,E-MTAB-7476,Transcriptome Analysis,Tissues specific transcriptomes of Danio rerio Danio albolineatus and Danio aesculapii Brain Liver and Gonads.,ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05,,Protocols: Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers.,R1wB,SAMEA5147907,"University of Hamburg, Institute of Zoology, Molecular Animal Physiology",ENA FIRST PUBLIC:2018 12 05T17:02:27Z|ENA LAST UPDATE:2018 12 05T10:01:48Z|External Id:SAMEA5147907|INSDC center name:University of Hamburg Institute of Zoology Molecular Animal Physiology|INSDC first public:2018 12 05T17:02:27Z|INSDC last update:2018 12 05T10:01:48Z|INSDC status:public|Submitter Id:E MTAB 7476:R1wB|age:3|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|individual:1|organism part:brain|sample name:E MTAB 7476:R1wB|scientific name:Danio rerio|sex:female|strain:tu,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danio9 species,E MTAB 7476:R1wB p,R1wB p,RNA Seq of Danionine species,Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers.,Experimental Factor: organism:Danio rerio|Experimental Factor: organism part:brain,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina HiSeq 4000,2000FApplication ReadForward11RApplication ReadReverse101,ERP112513,Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danionine species,ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05,FCHNW2WBBXX_L6_HKZEBodsEAAARAAPEI-202_1.fq.gz FCHNW2WBBXX_L6_HKZEBodsEAAARAAPEI-202_2.fq.gz,fastq fastq,3345687800.0,16728439.0,E MTAB 7476:FCHNW2WBBXX L6 HKZEBodsEAAARAAPEI 202 ,0:100 1:100,A:879841532;C:789462135;G:789187239;T:886468300;N:728594,100,100,,,879841532,789462135,789187239,886468300,728594,ERX2986064,ERS2955652,ERA1674470,"University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive","University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive",2,0.96514,0.96503,0.0325,0.0326,0.78967,0.79204,0.25943,0.26683,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2018-12-05,Adult,Adult,Brain,Nervous System
10176,ERR5858457,ERX5504346,ERS6343450,ERP128749,PRJEB44676,scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,E-MTAB-10390,Transcriptome Analysis,To analyse lesion induced gene regulation in progenitor cells at single cell resolution we performed single cell RNAseq on FACS isolated her4.3:GFP progenitor cells from the spinal cord at 24 hours post lesion hpl post spinal injury at 3 dpf dpf compared to age matched uninjured animals.,ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24,,Protocols: Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.,Naive,SAMEA8658904,University Of Edinburgh,ENA first public:2021 05 24|ENA last update:2021 05 24|External Id:SAMEA8658904|INSDC center alias:UOE|INSDC center name:University Of Edinburgh|INSDC first public:2021 05 24T00:14:32Z|INSDC last update:2021 05 24T00:14:32Z|INSDC status:public|Submitter Id:E MTAB 10390:Naive|age:4|broker name:ArrayExpress|cell type:ependymo radial glial cell|common name:zebrafish|developmental stage:larval day 4|immunophenotype:Her4.3+ positive|organism part:spinal cord|sample name:E MTAB 10390:Naive|sex:mixed|strain:WIK,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesi1d and unlesi1d zebrafish larvae spinal cord,E MTAB 10390:Naive p,Naive p,scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.,Experimental Factor: injury:n1,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP128749,Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24,Naive.bam,bam,44972162730.0,499690697.0,E MTAB 10390:Naive,0:90,A:13658826375;C:8733304343;G:9348137569;T:13228064343;N:3830100,90,,,,13658826375,8733304343,9348137569,13228064343,3830100,ERX5504346,ERS6343450,ERA4142789,University Of Edinburgh|European Nucleotide Archive,University Of Edinburgh|European Nucleotide Archive,1,0.89428,,0.32693,,0.75276,,0.5314,,90,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,sc,single_cell_droplet,10x,,United Kingdom,2021-05-24,Larval,Larval,Spinal Cord,Nervous System
10177,ERR5858456,ERX5504345,ERS6343449,ERP128749,PRJEB44676,scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,E-MTAB-10390,Transcriptome Analysis,To analyse lesion induced gene regulation in progenitor cells at single cell resolution we performed single cell RNAseq on FACS isolated her4.3:GFP progenitor cells from the spinal cord at 24 hours post lesion hpl post spinal injury at 3 dpf dpf compared to age matched uninjured animals.,ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24,,Protocols: Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.,Lesi1d,SAMEA8658903,University Of Edinburgh,ENA first public:2021 05 24|ENA last update:2021 05 24|External Id:SAMEA8658903|INSDC center alias:UOE|INSDC center name:University Of Edinburgh|INSDC first public:2021 05 24T00:14:32Z|INSDC last update:2021 05 24T00:14:32Z|INSDC status:public|Submitter Id:E MTAB 10390:Lesi1d|age:4|broker name:ArrayExpress|cell type:ependymo radial glial cell|common name:zebrafish|developmental stage:larval day 4|immunophenotype:Her4.3+ positive|injury:spinal injury lesion|organism part:spinal cord|sample name:E MTAB 10390:Lesi1d|sex:mixed|strain:WIK,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesi1d and unlesi1d zebrafish larvae spinal cord,E MTAB 10390:Lesioned p,Lesioned p,scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.,Experimental Factor: injury:spinal injury lesion,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP128749,Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24,Lesioned.bam,bam,49902588630.0,554473207.0,E MTAB 10390:Lesioned,0:90,A:14713351178;C:10191139050;G:10897887675;T:14095967201;N:4243526,90,,,,14713351178,10191139050,10897887675,14095967201,4243526,ERX5504345,ERS6343449,ERA4142789,University Of Edinburgh|European Nucleotide Archive,University Of Edinburgh|European Nucleotide Archive,1,0.91197,,0.29137,,0.7568,,0.56523,,90,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,sc,single_cell_droplet,10x,,United Kingdom,2021-05-24,Larval,Larval,Spinal Cord,Nervous System
10237,ERR7131169,ERX6698608,ERS8070397,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 Nega,SAMEA10418613,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 Nega s,F8 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F22-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L006_R1_001.fastq.gz,fastq,658093749.0,12903799.0,E MTAB 11083:2874F22 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L006,0:51 1:0,A:173355262;C:152464129;G:147489024;T:184739885;N:45449,51,0,,,173355262,152464129,147489024,184739885,45449,ERX6698608,ERS8070397,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78328,,0.15147,,0.71289,,0.53671,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10238,ERR7131170,ERX6698608,ERS8070397,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 Nega,SAMEA10418613,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 Nega s,F8 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F22-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L007_R1_001.fastq.gz,fastq,661103769.0,12962819.0,E MTAB 11083:2874F22 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L007,0:51 1:0,A:174223249;C:153223284;G:148276805;T:185335092;N:45339,51,0,,,174223249,153223284,148276805,185335092,45339,ERX6698608,ERS8070397,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78539,,0.15054,,0.70897,,0.5322,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10239,ERR7131167,ERX6698607,ERS8070396,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 mCherry GFP,SAMEA10418612,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry GFP s,F8 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F24-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L006_R1_001.fastq.gz,fastq,693183636.0,13591836.0,E MTAB 11083:2874F24 1 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L006,0:51 1:0,A:183977687;C:159275153;G:153149826;T:196732022;N:48948,51,0,,,183977687,159275153,153149826,196732022,48948,ERX6698607,ERS8070396,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68942,,0.15593,,0.75828,,0.51858,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10240,ERR7131168,ERX6698607,ERS8070396,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 mCherry GFP,SAMEA10418612,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry GFP s,F8 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F24-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L007_R1_001.fastq.gz,fastq,696648678.0,13659778.0,E MTAB 11083:2874F24 2 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L007,0:51 1:0,A:184971832;C:160126524;G:154012023;T:197490098;N:48201,51,0,,,184971832,160126524,154012023,197490098,48201,ERX6698607,ERS8070396,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.6906,,0.15634,,0.75909,,0.51346,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10241,ERR7131165,ERX6698606,ERS8070395,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 mCherry,SAMEA10418611,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry s,F8 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F23-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L006_R1_001.fastq.gz,fastq,667760646.0,13093346.0,E MTAB 11083:2874F23 1 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L006,0:51 1:0,A:179948739;C:150541918;G:145038845;T:192184092;N:47052,51,0,,,179948739,150541918,145038845,192184092,47052,ERX6698606,ERS8070395,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71661,,0.19774,,0.74576,,0.52117,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10242,ERR7131166,ERX6698606,ERS8070395,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 mCherry,SAMEA10418611,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry s,F8 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F23-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L007_R1_001.fastq.gz,fastq,670970484.0,13156284.0,E MTAB 11083:2874F23 2 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L007,0:51 1:0,A:180906557;C:151329492;G:145816347;T:192872804;N:45284,51,0,,,180906557,151329492,145816347,192872804,45284,ERX6698606,ERS8070395,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71682,,0.19902,,0.74517,,0.52618,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10243,ERR7131163,ERX6698605,ERS8070394,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 Nega,SAMEA10418610,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 Nega s,F7 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F19-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L006_R1_001.fastq.gz,fastq,652205238.0,12788338.0,E MTAB 11083:2874F19 1 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L006,0:51 1:0,A:173730405;C:149044598;G:145436777;T:183946928;N:46530,51,0,,,173730405,149044598,145436777,183946928,46530,ERX6698605,ERS8070394,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80595,,0.17309,,0.71003,,0.53696,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10244,ERR7131164,ERX6698605,ERS8070394,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 Nega,SAMEA10418610,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 Nega s,F7 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F19-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L007_R1_001.fastq.gz,fastq,655593423.0,12854773.0,E MTAB 11083:2874F19 2 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L007,0:51 1:0,A:174715119;C:149844974;G:146250446;T:184737535;N:45349,51,0,,,174715119,149844974,146250446,184737535,45349,ERX6698605,ERS8070394,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80585,,0.17497,,0.71078,,0.53767,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10245,ERR7131161,ERX6698604,ERS8070393,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 mCherry GFP,SAMEA10418609,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry GFP s,F7 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F21-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L006_R1_001.fastq.gz,fastq,661067151.0,12962101.0,E MTAB 11083:2874F21 1 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L006,0:51 1:0,A:168281419;C:157701209;G:153487197;T:181550252;N:47074,51,0,,,168281419,157701209,153487197,181550252,47074,ERX6698604,ERS8070393,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.39074,,0.11592,,0.82615,,0.5252,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10246,ERR7131162,ERX6698604,ERS8070393,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 mCherry GFP,SAMEA10418609,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry GFP s,F7 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F21-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L007_R1_001.fastq.gz,fastq,665709018.0,13053118.0,E MTAB 11083:2874F21 2 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L007,0:51 1:0,A:169539998;C:158884603;G:154624803;T:182614264;N:45350,51,0,,,169539998,158884603,154624803,182614264,45350,ERX6698604,ERS8070393,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.38966,,0.1152,,0.8258,,0.53305,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10247,ERR7131159,ERX6698603,ERS8070392,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 mCherry,SAMEA10418608,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry s,F7 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F20-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L006_R1_001.fastq.gz,fastq,657813402.0,12898302.0,E MTAB 11083:2874F20 1 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L006,0:51 1:0,A:175873489;C:149476129;G:143987770;T:188429588;N:46426,51,0,,,175873489,149476129,143987770,188429588,46426,ERX6698603,ERS8070392,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70507,,0.2048,,0.75923,,0.52828,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10248,ERR7131160,ERX6698603,ERS8070392,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 mCherry,SAMEA10418608,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry s,F7 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F20-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L007_R1_001.fastq.gz,fastq,660303426.0,12947126.0,E MTAB 11083:2874F20 2 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L007,0:51 1:0,A:176607119;C:150065781;G:144613110;T:188972809;N:44607,51,0,,,176607119,150065781,144613110,188972809,44607,ERX6698603,ERS8070392,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70705,,0.20314,,0.75852,,0.52949,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10249,ERR7131157,ERX6698602,ERS8070391,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 Nega,SAMEA10418607,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 Nega s,F6 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F16-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L006_R1_001.fastq.gz,fastq,669627450.0,13129950.0,E MTAB 11083:2874F16 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L006,0:51 1:0,A:177483937;C:154097051;G:148063012;T:189935690;N:47760,51,0,,,177483937,154097051,148063012,189935690,47760,ERX6698602,ERS8070391,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.77183,,0.16001,,0.71467,,0.53352,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10250,ERR7131158,ERX6698602,ERS8070391,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 Nega,SAMEA10418607,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 Nega s,F6 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F16-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L007_R1_001.fastq.gz,fastq,671615073.0,13168923.0,E MTAB 11083:2874F16 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L007,0:51 1:0,A:178109157;C:154619024;G:148595207;T:190246469;N:45216,51,0,,,178109157,154619024,148595207,190246469,45216,ERX6698602,ERS8070391,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.77284,,0.15963,,0.71569,,0.53038,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10251,ERR7131155,ERX6698601,ERS8070390,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 mCherry GFP,SAMEA10418606,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry GFP s,F6 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F18-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L006_R1_001.fastq.gz,fastq,667663542.0,13091442.0,E MTAB 11083:2874F18 1 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L006,0:51 1:0,A:177646483;C:152747528;G:146388226;T:190833951;N:47354,51,0,,,177646483,152747528,146388226,190833951,47354,ERX6698601,ERS8070390,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67964,,0.18123,,0.77193,,0.53313,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10252,ERR7131156,ERX6698601,ERS8070390,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 mCherry GFP,SAMEA10418606,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry GFP s,F6 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F18-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L007_R1_001.fastq.gz,fastq,670170141.0,13140591.0,E MTAB 11083:2874F18 2 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L007,0:51 1:0,A:178425395;C:153363855;G:147033779;T:191300976;N:46136,51,0,,,178425395,153363855,147033779,191300976,46136,ERX6698601,ERS8070390,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67922,,0.18101,,0.77141,,0.53601,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10253,ERR7131153,ERX6698600,ERS8070389,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 mCherry,SAMEA10418605,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry s,F6 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F17-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L006_R1_001.fastq.gz,fastq,677617008.0,13286608.0,E MTAB 11083:2874F17 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L006,0:51 1:0,A:181785899;C:152797687;G:147070676;T:195914808;N:47938,51,0,,,181785899,152797687,147070676,195914808,47938,ERX6698600,ERS8070389,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68298,,0.1953,,0.76292,,0.5395,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10254,ERR7131154,ERX6698600,ERS8070389,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 mCherry,SAMEA10418605,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry s,F6 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F17-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L007_R1_001.fastq.gz,fastq,679922973.0,13331823.0,E MTAB 11083:2874F17 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L007,0:51 1:0,A:182567819;C:153380607;G:147589890;T:196337722;N:46935,51,0,,,182567819,153380607,147589890,196337722,46935,ERX6698600,ERS8070389,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68391,,0.19726,,0.76299,,0.54099,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10255,ERR7131151,ERX6698599,ERS8070388,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 Nega,SAMEA10418604,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 Nega s,F5 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F13-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L006_R1_001.fastq.gz,fastq,620316315.0,12163065.0,E MTAB 11083:2874F13 1 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L006,0:51 1:0,A:165967355;C:141072216;G:136931219;T:176301963;N:43562,51,0,,,165967355,141072216,136931219,176301963,43562,ERX6698599,ERS8070388,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78924,,0.17457,,0.71934,,0.53563,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10256,ERR7131152,ERX6698599,ERS8070388,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 Nega,SAMEA10418604,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 Nega s,F5 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F13-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L007_R1_001.fastq.gz,fastq,623905338.0,12233438.0,E MTAB 11083:2874F13 2 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L007,0:51 1:0,A:166996253;C:141937637;G:137825206;T:177103504;N:42738,51,0,,,166996253,141937637,137825206,177103504,42738,ERX6698599,ERS8070388,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78975,,0.17387,,0.72153,,0.54149,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10257,ERR7131149,ERX6698598,ERS8070387,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 mCherry GFP,SAMEA10418603,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry GFP s,F5 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F15-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L006_R1_001.fastq.gz,fastq,670154841.0,13140291.0,E MTAB 11083:2874F15 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L006,0:51 1:0,A:182457480;C:149175456;G:144548173;T:193927470;N:46262,51,0,,,182457480,149175456,144548173,193927470,46262,ERX6698598,ERS8070387,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71875,,0.18671,,0.76047,,0.52398,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10258,ERR7131150,ERX6698598,ERS8070387,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 mCherry GFP,SAMEA10418603,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry GFP s,F5 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F15-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L007_R1_001.fastq.gz,fastq,674202048.0,13219648.0,E MTAB 11083:2874F15 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L007,0:51 1:0,A:183639511;C:150168226;G:145502071;T:194847755;N:44485,51,0,,,183639511,150168226,145502071,194847755,44485,ERX6698598,ERS8070387,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.72111,,0.18813,,0.76378,,0.5258,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10259,ERR7131147,ERX6698597,ERS8070386,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 mCherry,SAMEA10418602,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry s,F5 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F14-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L006_R1_001.fastq.gz,fastq,615458259.0,12067809.0,E MTAB 11083:2874F14 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L006,0:51 1:0,A:165108582;C:139178883;G:134070672;T:177059757;N:40365,51,0,,,165108582,139178883,134070672,177059757,40365,ERX6698597,ERS8070386,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69017,,0.1977,,0.77193,,0.53352,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10260,ERR7131148,ERX6698597,ERS8070386,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 mCherry,SAMEA10418602,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry s,F5 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F14-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L007_R1_001.fastq.gz,fastq,619061613.0,12138463.0,E MTAB 11083:2874F14 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L007,0:51 1:0,A:166167516;C:140058774;G:134972850;T:177822733;N:39740,51,0,,,166167516,140058774,134972850,177822733,39740,ERX6698597,ERS8070386,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69118,,0.20019,,0.7707,,0.52174,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10261,ERR7131145,ERX6698596,ERS8070385,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 Nega,SAMEA10418601,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 Nega s,F4 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F10-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L006_R1_001.fastq.gz,fastq,652536687.0,12794837.0,E MTAB 11083:2874F10 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L006,0:51 1:0,A:178033231;C:144661707;G:139876661;T:189921085;N:44003,51,0,,,178033231,144661707,139876661,189921085,44003,ERX6698596,ERS8070385,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.76201,,0.2508,,0.71299,,0.53379,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10262,ERR7131146,ERX6698596,ERS8070385,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 Nega,SAMEA10418601,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 Nega s,F4 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F10-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L007_R1_001.fastq.gz,fastq,655278957.0,12848607.0,E MTAB 11083:2874F10 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L007,0:51 1:0,A:178938002;C:145332613;G:140527756;T:190437189;N:43397,51,0,,,178938002,145332613,140527756,190437189,43397,ERX6698596,ERS8070385,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.76188,,0.25356,,0.71344,,0.53081,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10263,ERR7131143,ERX6698595,ERS8070384,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 mCherry GFP,SAMEA10418600,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry GFP s,F4 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F12-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L006_R1_001.fastq.gz,fastq,297921855.0,5841605.0,E MTAB 11083:2874F12 1 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L006,0:51 1:0,A:82400724;C:65517496;G:64603058;T:85384480;N:16097,51,0,,,82400724,65517496,64603058,85384480,16097,ERX6698595,ERS8070384,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.72496,,0.20794,,0.81223,,0.52529,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10264,ERR7131144,ERX6698595,ERS8070384,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 mCherry GFP,SAMEA10418600,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry GFP s,F4 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F12-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L007_R1_001.fastq.gz,fastq,309412971.0,6066921.0,E MTAB 11083:2874F12 2 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L007,0:51 1:0,A:85531271;C:68164437;G:67129209;T:88573210;N:14844,51,0,,,85531271,68164437,67129209,88573210,14844,ERX6698595,ERS8070384,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.7245,,0.20724,,0.80468,,0.52791,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10265,ERR7131141,ERX6698594,ERS8070383,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 mCherry,SAMEA10418599,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry s,F4 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F11-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L006_R1_001.fastq.gz,fastq,672319791.0,13182741.0,E MTAB 11083:2874F11 1 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L006,0:51 1:0,A:181508034;C:150928549;G:146124085;T:193711358;N:47765,51,0,,,181508034,150928549,146124085,193711358,47765,ERX6698594,ERS8070383,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71726,,0.19842,,0.74986,,0.53363,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10266,ERR7131142,ERX6698594,ERS8070383,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 mCherry,SAMEA10418599,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry s,F4 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F11-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L007_R1_001.fastq.gz,fastq,675486075.0,13244825.0,E MTAB 11083:2874F11 2 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L007,0:51 1:0,A:182462261;C:151688554;G:146902713;T:194385945;N:46602,51,0,,,182462261,151688554,146902713,194385945,46602,ERX6698594,ERS8070383,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71745,,0.19727,,0.74805,,0.53092,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10267,ERR7131139,ERX6698593,ERS8070382,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 Nega,SAMEA10418598,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 Nega s,F3 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F7-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L006_R1_001.fastq.gz,fastq,630093627.0,12354777.0,E MTAB 11083:2874F7 1 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L006,0:51 1:0,A:165132739;C:146179836;G:142796880;T:175940613;N:43559,51,0,,,165132739,146179836,142796880,175940613,43559,ERX6698593,ERS8070382,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81672,,0.15219,,0.71277,,0.52461,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10268,ERR7131140,ERX6698593,ERS8070382,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 Nega,SAMEA10418598,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 Nega s,F3 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F7-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L007_R1_001.fastq.gz,fastq,632305242.0,12398142.0,E MTAB 11083:2874F7 2 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L007,0:51 1:0,A:165832174;C:146751895;G:143384539;T:176293313;N:43321,51,0,,,165832174,146751895,143384539,176293313,43321,ERX6698593,ERS8070382,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81791,,0.15444,,0.7151,,0.52585,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10269,ERR7131137,ERX6698592,ERS8070381,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 mCherry GFP,SAMEA10418597,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry GFP s,F3 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F9-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L006_R1_001.fastq.gz,fastq,660379620.0,12948620.0,E MTAB 11083:2874F9 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L006,0:51 1:0,A:178482695;C:147417787;G:143797476;T:190635173;N:46489,51,0,,,178482695,147417787,143797476,190635173,46489,ERX6698592,ERS8070381,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.65635,,0.17778,,0.76899,,0.52957,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10270,ERR7131138,ERX6698592,ERS8070381,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 mCherry GFP,SAMEA10418597,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry GFP s,F3 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F9-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L007_R1_001.fastq.gz,fastq,662411307.0,12988457.0,E MTAB 11083:2874F9 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L007,0:51 1:0,A:179158323;C:147942300;G:144319925;T:190945370;N:45389,51,0,,,179158323,147942300,144319925,190945370,45389,ERX6698592,ERS8070381,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.65813,,0.17896,,0.77076,,0.52835,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10271,ERR7131135,ERX6698591,ERS8070380,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 mCherry,SAMEA10418596,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry s,F3 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F8-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L006_R1_001.fastq.gz,fastq,698608353.0,13698203.0,E MTAB 11083:2874F8 1 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L006,0:51 1:0,A:188718612;C:156814081;G:151125083;T:201901570;N:49007,51,0,,,188718612,156814081,151125083,201901570,49007,ERX6698591,ERS8070380,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70185,,0.21547,,0.75588,,0.53853,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10272,ERR7131136,ERX6698591,ERS8070380,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 mCherry,SAMEA10418596,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry s,F3 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F8-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L007_R1_001.fastq.gz,fastq,699869175.0,13722925.0,E MTAB 11083:2874F8 2 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L007,0:51 1:0,A:189192315;C:157176897;G:151498983;T:201952867;N:48113,51,0,,,189192315,157176897,151498983,201952867,48113,ERX6698591,ERS8070380,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70221,,0.21684,,0.75621,,0.53787,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10273,ERR7131133,ERX6698590,ERS8070379,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 Nega,SAMEA10418595,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 Nega s,F2 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F4-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L006_R1_001.fastq.gz,fastq,573812985.0,11251235.0,E MTAB 11083:2874F4 1 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L006,0:51 1:0,A:150716404;C:132203167;G:129732968;T:161120057;N:40389,51,0,,,150716404,132203167,129732968,161120057,40389,ERX6698590,ERS8070379,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81899,,0.17302,,0.72281,,0.54315,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10274,ERR7131134,ERX6698590,ERS8070379,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 Nega,SAMEA10418595,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 Nega s,F2 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F4-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L007_R1_001.fastq.gz,fastq,576184587.0,11297737.0,E MTAB 11083:2874F4 2 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L007,0:51 1:0,A:151387408;C:132788210;G:130392043;T:161577529;N:39397,51,0,,,151387408,132788210,130392043,161577529,39397,ERX6698590,ERS8070379,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81999,,0.17082,,0.72196,,0.54505,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10275,ERR7131131,ERX6698589,ERS8070378,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 mCherry GFP,SAMEA10418594,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry GFP s,F2 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F6-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L006_R1_001.fastq.gz,fastq,654811032.0,12839432.0,E MTAB 11083:2874F6 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L006,0:51 1:0,A:177403486;C:146202733;G:142207545;T:188951373;N:45895,51,0,,,177403486,146202733,142207545,188951373,45895,ERX6698589,ERS8070378,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67854,,0.21407,,0.76104,,0.52713,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10276,ERR7131132,ERX6698589,ERS8070378,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 mCherry GFP,SAMEA10418594,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry GFP s,F2 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F6-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L007_R1_001.fastq.gz,fastq,656911926.0,12880626.0,E MTAB 11083:2874F6 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L007,0:51 1:0,A:178119212;C:146730132;G:142725348;T:189293212;N:44022,51,0,,,178119212,146730132,142725348,189293212,44022,ERX6698589,ERS8070378,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68162,,0.21431,,0.761,,0.5071,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10277,ERR7131129,ERX6698588,ERS8070377,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 mCherry,SAMEA10418593,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry s,F2 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F5-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L006_R1_001.fastq.gz,fastq,607851558.0,11918658.0,E MTAB 11083:2874F5 1 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L006,0:51 1:0,A:164814138;C:136071648;G:132945950;T:173976881;N:42941,51,0,,,164814138,136071648,132945950,173976881,42941,ERX6698588,ERS8070377,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78967,,0.18905,,0.73241,,0.52347,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10278,ERR7131130,ERX6698588,ERS8070377,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 mCherry,SAMEA10418593,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry s,F2 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F5-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L007_R1_001.fastq.gz,fastq,610931805.0,11979055.0,E MTAB 11083:2874F5 2 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L007,0:51 1:0,A:165714775;C:136822925;G:133684294;T:174667814;N:41997,51,0,,,165714775,136822925,133684294,174667814,41997,ERX6698588,ERS8070377,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78869,,0.18885,,0.73156,,0.51691,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10279,ERR7131127,ERX6698587,ERS8070376,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 Nega,SAMEA10418592,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 Nega s,F1 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F1-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L006_R1_001.fastq.gz,fastq,619561872.0,12148272.0,E MTAB 11083:2874F1 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L006,0:51 1:0,A:164385545;C:141746313;G:138163219;T:175223323;N:43472,51,0,,,164385545,141746313,138163219,175223323,43472,ERX6698587,ERS8070376,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80258,,0.17703,,0.72614,,0.53483,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10280,ERR7131128,ERX6698587,ERS8070376,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 Nega,SAMEA10418592,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 Nega s,F1 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F1-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L007_R1_001.fastq.gz,fastq,621481971.0,12185921.0,E MTAB 11083:2874F1 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L007,0:51 1:0,A:165030774;C:142231469;G:138651078;T:175527080;N:41570,51,0,,,165030774,142231469,138651078,175527080,41570,ERX6698587,ERS8070376,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80248,,0.17699,,0.726,,0.53711,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10281,ERR7131125,ERX6698586,ERS8070375,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 mCherry GFP,SAMEA10418591,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry GFP s,F1 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F3-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L006_R1_001.fastq.gz,fastq,651189369.0,12768419.0,E MTAB 11083:2874F3 1 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L006,0:51 1:0,A:176398415;C:145659111;G:142137610;T:186948280;N:45953,51,0,,,176398415,145659111,142137610,186948280,45953,ERX6698586,ERS8070375,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69561,,0.18489,,0.75345,,0.52316,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10282,ERR7131126,ERX6698586,ERS8070375,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 mCherry GFP,SAMEA10418591,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry GFP s,F1 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F3-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L007_R1_001.fastq.gz,fastq,653398944.0,12811744.0,E MTAB 11083:2874F3 2 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L007,0:51 1:0,A:177139734;C:146171766;G:142707146;T:187335475;N:44823,51,0,,,177139734,146171766,142707146,187335475,44823,ERX6698586,ERS8070375,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69496,,0.18414,,0.75375,,0.52317,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10283,ERR7131123,ERX6698585,ERS8070374,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 mCherry,SAMEA10418590,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry s,F1 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F2-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L006_R1_001.fastq.gz,fastq,613574319.0,12030869.0,E MTAB 11083:2874F2 1 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L006,0:51 1:0,A:164891629;C:138401759;G:135818073;T:174419828;N:43030,51,0,,,164891629,138401759,135818073,174419828,43030,ERX6698585,ERS8070374,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.79499,,0.21674,,0.73359,,0.52207,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10284,ERR7131124,ERX6698585,ERS8070374,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 mCherry,SAMEA10418590,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry s,F1 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F2-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L007_R1_001.fastq.gz,fastq,615666798.0,12071898.0,E MTAB 11083:2874F2 2 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L007,0:51 1:0,A:165536502;C:138952758;G:136375193;T:174760659;N:41686,51,0,,,165536502,138952758,136375193,174760659,41686,ERX6698585,ERS8070374,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.7941,,0.21645,,0.73494,,0.50761,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
11046,ERR10476843,ERX9997186,ERS13672511,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 3 brains,star:bPAC / whole brain day120+LD stress,SAMEA111562655,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg LD 2|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star neg LD 2|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:198 283105,Sample 0256 122 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_122_FR_NSP_TR1_SL1_S51_L001_R1_001-pooled.fastq.gz 0256_122_FR_NSP_TR1_SL1_S51_L001_R2_001-pooled.fastq.gz,fastq fastq,3396097242.0,33295071.0,ena RUN TAB 09 11 2022 11:52:59:199 283106,0:51 1:51,A:937395652;C:754621322;G:762999709;T:941041776;N:38783,51,51,,,937395652,754621322,762999709,941041776,38783,ERX9997186,ERS13672511,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11047,ERR10476807,ERX9997150,ERS13672475,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day6,SAMEA111562619,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d6 1|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star pos d6 1|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:183 283033,Sample 0256 076 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_076_FR_NSP_TR1_SL1_S7_L001_R1_001-pooled.fastq.gz 0256_076_FR_NSP_TR1_SL1_S7_L001_R2_001-pooled.fastq.gz,fastq fastq,2974117224.0,29158012.0,ena RUN TAB 09 11 2022 11:52:59:183 283034,0:51 1:51,A:816102587;C:662715848;G:672777315;T:822487687;N:33787,51,51,,,816102587,662715848,672777315,822487687,33787,ERX9997150,ERS13672475,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11048,ERR10476845,ERX9997188,ERS13672513,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 3 brains,star:bPAC / whole brain day120+LD stress,SAMEA111562657,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg LD 4|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star neg LD 4|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:199 283109,Sample 0256 124 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_124_FR_NSP_TR1_SL1_S53_L001_R1_001-pooled.fastq.gz 0256_124_FR_NSP_TR1_SL1_S53_L001_R2_001-pooled.fastq.gz,fastq fastq,2964111330.0,29059915.0,ena RUN TAB 09 11 2022 11:52:59:199 283110,0:51 1:51,A:822335161;C:651614200;G:667534457;T:822593427;N:34085,51,51,,,822335161,651614200,667534457,822593427,34085,ERX9997188,ERS13672513,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11049,ERR10476819,ERX9997162,ERS13672487,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120,SAMEA111562631,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d120 3|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos d120 3|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:188 283057,Sample 0256 093 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_093_FR_NSP_TR1_SL1_S24_L001_R1_001-pooled.fastq.gz 0256_093_FR_NSP_TR1_SL1_S24_L001_R2_001-pooled.fastq.gz,fastq fastq,3376444086.0,33102393.0,ena RUN TAB 09 11 2022 11:52:59:188 283058,0:51 1:51,A:938993037;C:741979766;G:744362740;T:951069922;N:38621,51,51,,,938993037,741979766,744362740,951069922,38621,ERX9997162,ERS13672487,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11050,ERR10476814,ERX9997157,ERS13672482,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day13,SAMEA111562626,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d13 3|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star pos d13 3|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:186 283047,Sample 0256 083 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_083_FR_NSP_TR2_SL1_S14_L001_R1_001-pooled.fastq.gz 0256_083_FR_NSP_TR2_SL1_S14_L001_R2_001-pooled.fastq.gz,fastq fastq,5492667156.0,53849678.0,ena RUN TAB 09 11 2022 11:52:59:186 283048,0:51 1:51,A:1546610275;C:1178241230;G:1195797489;T:1571955787;N:62375,51,51,,,1546610275,1178241230,1195797489,1571955787,62375,ERX9997157,ERS13672482,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11051,ERR10476829,ERX9997172,ERS13672497,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day6,SAMEA111562641,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d6 3|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star neg d6 3|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:192 283077,Sample 0256 103 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_103_FR_NSP_TR1_SL1_S32_L001_R1_001-pooled.fastq.gz 0256_103_FR_NSP_TR1_SL1_S32_L001_R2_001-pooled.fastq.gz,fastq fastq,3120070248.0,30588924.0,ena RUN TAB 09 11 2022 11:52:59:193 283078,0:51 1:51,A:846274026;C:707353227;G:707836608;T:858570820;N:35567,51,51,,,846274026,707353227,707836608,858570820,35567,ERX9997172,ERS13672497,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11052,ERR10476798,ERX9997141,ERS13672466,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 3 brains,wildtype whole brain day120,SAMEA111562610,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d120 2|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:tu wt d120 2|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:179 283015,Sample 0256 017 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_017_FR_NSP_TR1_SL1_S28_L001_R1_001-pooled.fastq.gz 0256_017_FR_NSP_TR1_SL1_S28_L001_R2_001-pooled.fastq.gz,fastq fastq,2721824508.0,26684554.0,ena RUN TAB 09 11 2022 11:52:59:179 283016,0:51 1:51,A:743316669;C:608895552;G:628493804;T:741025191;N:93292,51,51,,,743316669,608895552,628493804,741025191,93292,ERX9997141,ERS13672466,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11053,ERR10476796,ERX9997139,ERS13672464,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day13,SAMEA111562608,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d13 5|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:tu wt d13 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:178 283011,Sample 0256 010 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_010_FR_NSP_TR1_SL1_S21_L001_R1_001-pooled.fastq.gz 0256_010_FR_NSP_TR1_SL1_S21_L001_R2_001-pooled.fastq.gz,fastq fastq,2969687772.0,29114586.0,ena RUN TAB 09 11 2022 11:52:59:179 283012,0:51 1:51,A:762435544;C:713336460;G:731717998;T:762096042;N:101728,51,51,,,762435544,713336460,731717998,762096042,101728,ERX9997139,ERS13672464,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11054,ERR10476824,ERX9997167,ERS13672492,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120+LD stress,SAMEA111562636,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos LD 3|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos LD 3|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:190 283067,Sample 0256 098 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_098_FR_NSP_TR1_SL1_S25_L001_R1_001-pooled.fastq.gz 0256_098_FR_NSP_TR1_SL1_S25_L001_R2_001-pooled.fastq.gz,fastq fastq,2446755498.0,23987799.0,ena RUN TAB 09 11 2022 11:52:59:190 283068,0:51 1:51,A:690730956;C:529182577;G:529532770;T:697255685;N:53510,51,51,,,690730956,529182577,529532770,697255685,53510,ERX9997167,ERS13672492,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11055,ERR10476836,ERX9997179,ERS13672504,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day13,SAMEA111562648,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d13 5|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star neg d13 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:195 283091,Sample 0256 110 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_110_FR_NSP_TR2_SL1_S39_L001_R1_001-pooled.fastq.gz 0256_110_FR_NSP_TR2_SL1_S39_L001_R2_001-pooled.fastq.gz,fastq fastq,2758547160.0,27044580.0,ena RUN TAB 09 11 2022 11:52:59:196 283092,0:51 1:51,A:732517446;C:640766914;G:643632251;T:741598802;N:31747,51,51,,,732517446,640766914,643632251,741598802,31747,ERX9997179,ERS13672504,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11056,ERR10476808,ERX9997151,ERS13672476,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day6,SAMEA111562620,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d6 2|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star pos d6 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:184 283035,Sample 0256 077 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_077_FR_NSP_TR1_SL1_S8_L001_R1_001-pooled.fastq.gz 0256_077_FR_NSP_TR1_SL1_S8_L001_R2_001-pooled.fastq.gz,fastq fastq,3595088328.0,35245964.0,ena RUN TAB 09 11 2022 11:52:59:184 283036,0:51 1:51,A:985480827;C:803117311;G:811895458;T:994553698;N:41034,51,51,,,985480827,803117311,811895458,994553698,41034,ERX9997151,ERS13672476,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11057,ERR10476818,ERX9997161,ERS13672486,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120,SAMEA111562630,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d120 2|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos d120 2|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:188 283055,Sample 0256 092 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_092_FR_NSP_TR1_SL1_S23_L001_R1_001-pooled.fastq.gz 0256_092_FR_NSP_TR1_SL1_S23_L001_R2_001-pooled.fastq.gz,fastq fastq,2928003330.0,28705915.0,ena RUN TAB 09 11 2022 11:52:59:188 283056,0:51 1:51,A:808470746;C:646721298;G:658265207;T:814512506;N:33573,51,51,,,808470746,646721298,658265207,814512506,33573,ERX9997161,ERS13672486,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11058,ERR10476827,ERX9997170,ERS13672495,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day6,SAMEA111562639,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d6 1|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star neg d6 1|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:192 283073,Sample 0256 101 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_101_FR_NSP_TR1_SL1_S30_L001_R1_001-pooled.fastq.gz 0256_101_FR_NSP_TR1_SL1_S30_L001_R2_001-pooled.fastq.gz,fastq fastq,3504125952.0,34354176.0,ena RUN TAB 09 11 2022 11:52:59:192 283074,0:51 1:51,A:947138693;C:797358353;G:798903844;T:960685108;N:39954,51,51,,,947138693,797358353,798903844,960685108,39954,ERX9997170,ERS13672495,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11059,ERR10476801,ERX9997144,ERS13672469,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 3 brains,wildtype whole brain day120,SAMEA111562613,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d120 5|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:tu wt d120 5|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:181 283021,Sample 0256 020 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_020_FR_NSP_TR1_SL1_S31_L001_R1_001-pooled.fastq.gz 0256_020_FR_NSP_TR1_SL1_S31_L001_R2_001-pooled.fastq.gz,fastq fastq,3216177708.0,31531154.0,ena RUN TAB 09 11 2022 11:52:59:181 283022,0:51 1:51,A:880463840;C:718401986;G:738221226;T:878980905;N:109751,51,51,,,880463840,718401986,738221226,878980905,109751,ERX9997144,ERS13672469,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11060,ERR10476794,ERX9997137,ERS13672462,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day13,SAMEA111562606,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d13 3|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:tu wt d13 3|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:178 283007,Sample 0256 008 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_008_FR_NSP_TR1_SL1_S1_L001_R1_001-pooled.fastq.gz 0256_008_FR_NSP_TR1_SL1_S1_L001_R2_001-pooled.fastq.gz,fastq fastq,2659023618.0,26068859.0,ena RUN TAB 09 11 2022 11:52:59:178 283008,0:51 1:51,A:670947753;C:637136773;G:677570266;T:673338670;N:30156,51,51,,,670947753,637136773,677570266,673338670,30156,ERX9997137,ERS13672462,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11061,ERR10476833,ERX9997176,ERS13672501,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day13,SAMEA111562645,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d13 2|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star neg d13 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:194 283085,Sample 0256 107 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_107_FR_NSP_TR2_SL1_S36_L001_R1_001-pooled.fastq.gz 0256_107_FR_NSP_TR2_SL1_S36_L001_R2_001-pooled.fastq.gz,fastq fastq,3282322362.0,32179631.0,ena RUN TAB 09 11 2022 11:52:59:194 283086,0:51 1:51,A:898607442;C:734331926;G:739939203;T:909406172;N:37619,51,51,,,898607442,734331926,739939203,909406172,37619,ERX9997176,ERS13672501,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11062,ERR10476817,ERX9997160,ERS13672485,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120,SAMEA111562629,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d120 1|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos d120 1|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:187 283053,Sample 0256 091 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_091_FR_NSP_TR1_SL1_S22_L001_R1_001-pooled.fastq.gz 0256_091_FR_NSP_TR1_SL1_S22_L001_R2_001-pooled.fastq.gz,fastq fastq,3195884706.0,31332203.0,ena RUN TAB 09 11 2022 11:52:59:188 283054,0:51 1:51,A:874879226;C:712315108;G:728957349;T:879696554;N:36469,51,51,,,874879226,712315108,728957349,879696554,36469,ERX9997160,ERS13672485,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11063,ERR10476828,ERX9997171,ERS13672496,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day6,SAMEA111562640,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d6 2|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star neg d6 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:192 283075,Sample 0256 102 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_102_FR_NSP_TR1_SL1_S31_L001_R1_001-pooled.fastq.gz 0256_102_FR_NSP_TR1_SL1_S31_L001_R2_001-pooled.fastq.gz,fastq fastq,3969901404.0,38920602.0,ena RUN TAB 09 11 2022 11:52:59:192 283076,0:51 1:51,A:1079447747;C:894502841;G:908706945;T:1087198510;N:45361,51,51,,,1079447747,894502841,908706945,1087198510,45361,ERX9997171,ERS13672496,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11064,ERR10476821,ERX9997164,ERS13672489,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120,SAMEA111562633,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d120 5|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos d120 5|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:189 283061,Sample 0256 095 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_095_FR_NSP_TR1_SL1_S26_L001_R1_001-pooled.fastq.gz 0256_095_FR_NSP_TR1_SL1_S26_L001_R2_001-pooled.fastq.gz,fastq fastq,3213304572.0,31502986.0,ena RUN TAB 09 11 2022 11:52:59:189 283062,0:51 1:51,A:891513837;C:705036538;G:716947743;T:899770077;N:36377,51,51,,,891513837,705036538,716947743,899770077,36377,ERX9997164,ERS13672489,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11065,ERR10476823,ERX9997166,ERS13672491,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120+LD stress,SAMEA111562635,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos LD 2|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos LD 2|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:190 283065,Sample 0256 097 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_097_FR_NSP_TR1_SL1_S24_L001_R1_001-pooled.fastq.gz 0256_097_FR_NSP_TR1_SL1_S24_L001_R2_001-pooled.fastq.gz,fastq fastq,2641036224.0,25892512.0,ena RUN TAB 09 11 2022 11:52:59:190 283066,0:51 1:51,A:757894363;C:555348260;G:569783120;T:757953770;N:56711,51,51,,,757894363,555348260,569783120,757953770,56711,ERX9997166,ERS13672491,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11066,ERR10476806,ERX9997149,ERS13672474,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 3 brains,wildtype whole brain day120+LD stress,SAMEA111562618,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt LD 5|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:tu wt LD 5|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:183 283031,Sample 0256 025 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_025_FR_NSP_TR1_SL1_S13_L001_R1_001-pooled.fastq.gz 0256_025_FR_NSP_TR1_SL1_S13_L001_R2_001-pooled.fastq.gz,fastq fastq,3305999418.0,32411759.0,ena RUN TAB 09 11 2022 11:52:59:183 283032,0:51 1:51,A:895024301;C:741182319;G:782231299;T:887499120;N:62379,51,51,,,895024301,741182319,782231299,887499120,62379,ERX9997149,ERS13672474,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11067,ERR10476846,ERX9997189,ERS13672514,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 3 brains,star:bPAC / whole brain day120+LD stress,SAMEA111562658,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg LD 5|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star neg LD 5|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:200 283111,Sample 0256 125 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_125_FR_NSP_TR1_SL1_S54_L001_R1_001-pooled.fastq.gz 0256_125_FR_NSP_TR1_SL1_S54_L001_R2_001-pooled.fastq.gz,fastq fastq,3282595008.0,32182304.0,ena RUN TAB 09 11 2022 11:52:59:200 283112,0:51 1:51,A:911207196;C:721152291;G:734350131;T:915848066;N:37324,51,51,,,911207196,721152291,734350131,915848066,37324,ERX9997189,ERS13672514,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11068,ERR10476790,ERX9997133,ERS13672458,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day6,SAMEA111562602,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d6 4|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:tu wt d6 4|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:176 282999,Sample 0256 004 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_004_FR_NSP_TR1_SL1_S16_L001_R1_001-pooled.fastq.gz 0256_004_FR_NSP_TR1_SL1_S16_L001_R2_001-pooled.fastq.gz,fastq fastq,2693489520.0,26406760.0,ena RUN TAB 09 11 2022 11:52:59:176 283000,0:51 1:51,A:701762127;C:639663261;G:643729664;T:708242984;N:91484,51,51,,,701762127,639663261,643729664,708242984,91484,ERX9997133,ERS13672458,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11069,ERR10476797,ERX9997140,ERS13672465,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 3 brains,wildtype whole brain day120,SAMEA111562609,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d120 1|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:tu wt d120 1|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:179 283013,Sample 0256 016 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_016_FR_NSP_TR1_SL1_S27_L001_R1_001-pooled.fastq.gz 0256_016_FR_NSP_TR1_SL1_S27_L001_R2_001-pooled.fastq.gz,fastq fastq,3238524072.0,31750236.0,ena RUN TAB 09 11 2022 11:52:59:179 283014,0:51 1:51,A:879431740;C:727298145;G:750466237;T:881218913;N:109037,51,51,,,879431740,727298145,750466237,881218913,109037,ERX9997140,ERS13672465,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11070,ERR10476791,ERX9997134,ERS13672459,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day6,SAMEA111562603,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d6 5|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:tu wt d6 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:176 283001,Sample 0256 005 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_005_FR_NSP_TR1_SL1_S17_L001_R1_001-pooled.fastq.gz 0256_005_FR_NSP_TR1_SL1_S17_L001_R2_001-pooled.fastq.gz,fastq fastq,2904430620.0,28474810.0,ena RUN TAB 09 11 2022 11:52:59:176 283002,0:51 1:51,A:777892611;C:666758312;G:672026589;T:787654140;N:98968,51,51,,,777892611,666758312,672026589,787654140,98968,ERX9997134,ERS13672459,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11071,ERR10476837,ERX9997180,ERS13672505,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 3 brains,star:bPAC / whole brain day120,SAMEA111562649,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d120 1|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star neg d120 1|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:196 283093,Sample 0256 116 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_116_FR_NSP_TR1_SL1_S45_L001_R1_001-pooled.fastq.gz 0256_116_FR_NSP_TR1_SL1_S45_L001_R2_001-pooled.fastq.gz,fastq fastq,3223186638.0,31599869.0,ena RUN TAB 09 11 2022 11:52:59:196 283094,0:51 1:51,A:887593868;C:714750043;G:727286570;T:893518987;N:37170,51,51,,,887593868,714750043,727286570,893518987,37170,ERX9997180,ERS13672505,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11072,ERR10476844,ERX9997187,ERS13672512,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 3 brains,star:bPAC / whole brain day120+LD stress,SAMEA111562656,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg LD 3|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star neg LD 3|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:199 283107,Sample 0256 123 FR NSP TR1 SL2,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_123_FR_NSP_TR1_SL2_S52_L001_R1_001-pooled.fastq.gz 0256_123_FR_NSP_TR1_SL2_S52_L001_R2_001-pooled.fastq.gz,fastq fastq,3363995598.0,32980349.0,ena RUN TAB 09 11 2022 11:52:59:199 283108,0:51 1:51,A:911801682;C:754249392;G:775610326;T:922295915;N:38283,51,51,,,911801682,754249392,775610326,922295915,38283,ERX9997187,ERS13672512,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11073,ERR10476841,ERX9997184,ERS13672509,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 3 brains,star:bPAC / whole brain day120,SAMEA111562653,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d120 5|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star neg d120 5|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:198 283101,Sample 0256 120 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_120_FR_NSP_TR1_SL1_S49_L001_R1_001-pooled.fastq.gz 0256_120_FR_NSP_TR1_SL1_S49_L001_R2_001-pooled.fastq.gz,fastq fastq,3183097374.0,31206837.0,ena RUN TAB 09 11 2022 11:52:59:198 283102,0:51 1:51,A:877463150;C:706812545;G:717353252;T:881432047;N:36380,51,51,,,877463150,706812545,717353252,881432047,36380,ERX9997184,ERS13672509,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11074,ERR10476788,ERX9997131,ERS13672456,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day6,SAMEA111562600,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d6 2|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:tu wt d6 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:175 282995,Sample 0256 002 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_002_FR_NSP_TR1_SL1_S14_L001_R1_001-pooled.fastq.gz 0256_002_FR_NSP_TR1_SL1_S14_L001_R2_001-pooled.fastq.gz,fastq fastq,2385303456.0,23385328.0,ena RUN TAB 09 11 2022 11:52:59:175 282996,0:51 1:51,A:620601269;C:567608030;G:568555174;T:628457831;N:81152,51,51,,,620601269,567608030,568555174,628457831,81152,ERX9997131,ERS13672456,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11075,ERR10476825,ERX9997168,ERS13672493,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120+LD stress,SAMEA111562637,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos LD 4|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos LD 4|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:191 283069,Sample 0256 099 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_099_FR_NSP_TR1_SL1_S28_L001_R1_001-pooled.fastq.gz 0256_099_FR_NSP_TR1_SL1_S28_L001_R2_001-pooled.fastq.gz,fastq fastq,3076257270.0,30159385.0,ena RUN TAB 09 11 2022 11:52:59:191 283070,0:51 1:51,A:873464149;C:655660863;G:671402896;T:875694442;N:34920,51,51,,,873464149,655660863,671402896,875694442,34920,ERX9997168,ERS13672493,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11076,ERR10476842,ERX9997185,ERS13672510,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 3 brains,star:bPAC / whole brain day120+LD stress,SAMEA111562654,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg LD 1|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star neg LD 1|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:198 283103,Sample 0256 121 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_121_FR_NSP_TR1_SL1_S50_L001_R1_001-pooled.fastq.gz 0256_121_FR_NSP_TR1_SL1_S50_L001_R2_001-pooled.fastq.gz,fastq fastq,2812259034.0,27571167.0,ena RUN TAB 09 11 2022 11:52:59:198 283104,0:51 1:51,A:777159410;C:623161405;G:635673358;T:776232920;N:31941,51,51,,,777159410,623161405,635673358,776232920,31941,ERX9997185,ERS13672510,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11077,ERR10476816,ERX9997159,ERS13672484,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day13,SAMEA111562628,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d13 5|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star pos d13 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:187 283051,Sample 0256 085 FR NSP TRP SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_085_FR_NSP_TRP_SL1_S16_L001_R1_001-pooled.fastq.gz 0256_085_FR_NSP_TRP_SL1_S16_L001_R2_001-pooled.fastq.gz,fastq fastq,3264856596.0,32008398.0,ena RUN TAB 09 11 2022 11:52:59:187 283052,0:51 1:51,A:908889213;C:708892816;G:727180747;T:919857088;N:36732,51,51,,,908889213,708892816,727180747,919857088,36732,ERX9997159,ERS13672484,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11078,ERR10476813,ERX9997156,ERS13672481,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day13,SAMEA111562625,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d13 2|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star pos d13 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:186 283045,Sample 0256 082 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_082_FR_NSP_TR2_SL1_S13_L001_R1_001-pooled.fastq.gz 0256_082_FR_NSP_TR2_SL1_S13_L001_R2_001-pooled.fastq.gz,fastq fastq,3037654248.0,29780924.0,ena RUN TAB 09 11 2022 11:52:59:186 283046,0:51 1:51,A:859112641;C:645943863;G:660257800;T:872305592;N:34352,51,51,,,859112641,645943863,660257800,872305592,34352,ERX9997156,ERS13672481,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11079,ERR10476832,ERX9997175,ERS13672500,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day13,SAMEA111562644,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d13 1|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star neg d13 1|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:194 283083,Sample 0256 106 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_106_FR_NSP_TR1_SL1_S35_L001_R1_001-pooled.fastq.gz 0256_106_FR_NSP_TR1_SL1_S35_L001_R2_001-pooled.fastq.gz,fastq fastq,2761703040.0,27075520.0,ena RUN TAB 09 11 2022 11:52:59:194 283084,0:51 1:51,A:761065450;C:610246142;G:622228689;T:768131440;N:31319,51,51,,,761065450,610246142,622228689,768131440,31319,ERX9997175,ERS13672500,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11080,ERR10476810,ERX9997153,ERS13672478,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day6,SAMEA111562622,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d6 4|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star pos d6 4|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:184 283039,Sample 0256 079 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_079_FR_NSP_TR1_SL1_S10_L001_R1_001-pooled.fastq.gz 0256_079_FR_NSP_TR1_SL1_S10_L001_R2_001-pooled.fastq.gz,fastq fastq,2745379674.0,26915487.0,ena RUN TAB 09 11 2022 11:52:59:185 283040,0:51 1:51,A:762974242;C:602287296;G:611894421;T:768192503;N:31212,51,51,,,762974242,602287296,611894421,768192503,31212,ERX9997153,ERS13672478,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11081,ERR10476811,ERX9997154,ERS13672479,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day6,SAMEA111562623,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d6 5|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star pos d6 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:185 283041,Sample 0256 080 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_080_FR_NSP_TR1_SL1_S11_L001_R1_001-pooled.fastq.gz 0256_080_FR_NSP_TR1_SL1_S11_L001_R2_001-pooled.fastq.gz,fastq fastq,3212151870.0,31491685.0,ena RUN TAB 09 11 2022 11:52:59:185 283042,0:51 1:51,A:890500591;C:706313900;G:715518225;T:899782352;N:36802,51,51,,,890500591,706313900,715518225,899782352,36802,ERX9997154,ERS13672479,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11082,ERR10476826,ERX9997169,ERS13672494,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120+LD stress,SAMEA111562638,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos LD 5|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos LD 5|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:191 283071,Sample 0256 100 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_100_FR_NSP_TR1_SL1_S29_L001_R1_001-pooled.fastq.gz 0256_100_FR_NSP_TR1_SL1_S29_L001_R2_001-pooled.fastq.gz,fastq fastq,3578369100.0,35082050.0,ena RUN TAB 09 11 2022 11:52:59:191 283072,0:51 1:51,A:1020283131;C:761534114;G:768445361;T:1028065987;N:40507,51,51,,,1020283131,761534114,768445361,1028065987,40507,ERX9997169,ERS13672494,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11083,ERR10476802,ERX9997145,ERS13672470,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 3 brains,wildtype whole brain day120+LD stress,SAMEA111562614,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt LD 1|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:tu wt LD 1|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:181 283023,Sample 0256 021 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_021_FR_NSP_TR1_SL1_S32_L001_R1_001-pooled.fastq.gz 0256_021_FR_NSP_TR1_SL1_S32_L001_R2_001-pooled.fastq.gz,fastq fastq,2990267394.0,29316347.0,ena RUN TAB 09 11 2022 11:52:59:181 283024,0:51 1:51,A:821947579;C:664213850;G:685635879;T:818367947;N:102139,51,51,,,821947579,664213850,685635879,818367947,102139,ERX9997145,ERS13672470,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11084,ERR10476799,ERX9997142,ERS13672467,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 3 brains,wildtype whole brain day120,SAMEA111562611,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d120 3|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:tu wt d120 3|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:180 283017,Sample 0256 018 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_018_FR_NSP_TR1_SL1_S29_L001_R1_001-pooled.fastq.gz 0256_018_FR_NSP_TR1_SL1_S29_L001_R2_001-pooled.fastq.gz,fastq fastq,2807137614.0,27520957.0,ena RUN TAB 09 11 2022 11:52:59:180 283018,0:51 1:51,A:773248982;C:621078395;G:638119915;T:774593844;N:96478,51,51,,,773248982,621078395,638119915,774593844,96478,ERX9997142,ERS13672467,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11085,ERR10476809,ERX9997152,ERS13672477,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day6,SAMEA111562621,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d6 3|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star pos d6 3|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:184 283037,Sample 0256 078 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_078_FR_NSP_TR1_SL1_S9_L001_R1_001-pooled.fastq.gz 0256_078_FR_NSP_TR1_SL1_S9_L001_R2_001-pooled.fastq.gz,fastq fastq,3412655106.0,33457403.0,ena RUN TAB 09 11 2022 11:52:59:184 283038,0:51 1:51,A:942783320;C:753155142;G:767154692;T:949522969;N:38983,51,51,,,942783320,753155142,767154692,949522969,38983,ERX9997152,ERS13672477,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11086,ERR10476789,ERX9997132,ERS13672457,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day6,SAMEA111562601,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d6 3|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:tu wt d6 3|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:175 282997,Sample 0256 003 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_003_FR_NSP_TR1_SL1_S15_L001_R1_001-pooled.fastq.gz 0256_003_FR_NSP_TR1_SL1_S15_L001_R2_001-pooled.fastq.gz,fastq fastq,2999816634.0,29409967.0,ena RUN TAB 09 11 2022 11:52:59:176 282998,0:51 1:51,A:771170539;C:720448182;G:729961101;T:778133283;N:103529,51,51,,,771170539,720448182,729961101,778133283,103529,ERX9997132,ERS13672457,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11087,ERR10476830,ERX9997173,ERS13672498,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day6,SAMEA111562642,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d6 4|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star neg d6 4|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:193 283079,Sample 0256 104 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_104_FR_NSP_TR1_SL1_S33_L001_R1_001-pooled.fastq.gz 0256_104_FR_NSP_TR1_SL1_S33_L001_R2_001-pooled.fastq.gz,fastq fastq,2943756210.0,28860355.0,ena RUN TAB 09 11 2022 11:52:59:193 283080,0:51 1:51,A:806343784;C:657990854;G:663721905;T:815666113;N:33554,51,51,,,806343784,657990854,663721905,815666113,33554,ERX9997173,ERS13672498,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11088,ERR10476835,ERX9997178,ERS13672503,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day13,SAMEA111562647,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d13 4|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star neg d13 4|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:195 283089,Sample 0256 109 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_109_FR_NSP_TR2_SL1_S38_L001_R1_001-pooled.fastq.gz 0256_109_FR_NSP_TR2_SL1_S38_L001_R2_001-pooled.fastq.gz,fastq fastq,3406677396.0,33398798.0,ena RUN TAB 09 11 2022 11:52:59:195 283090,0:51 1:51,A:928579532;C:768636951;G:771636154;T:937786376;N:38383,51,51,,,928579532,768636951,771636154,937786376,38383,ERX9997178,ERS13672503,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11089,ERR10476820,ERX9997163,ERS13672488,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 3 brains,star:bPAC+/ whole brain day120,SAMEA111562632,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d120 4|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star pos d120 4|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:189 283059,Sample 0256 094 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_094_FR_NSP_TR1_SL1_S25_L001_R1_001-pooled.fastq.gz 0256_094_FR_NSP_TR1_SL1_S25_L001_R2_001-pooled.fastq.gz,fastq fastq,3067693350.0,30075425.0,ena RUN TAB 09 11 2022 11:52:59:189 283060,0:51 1:51,A:855716246;C:671014617;G:674396566;T:866531062;N:34859,51,51,,,855716246,671014617,674396566,866531062,34859,ERX9997163,ERS13672488,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11090,ERR10476787,ERX9997130,ERS13672455,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day6,SAMEA111562599,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d6 1|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:tu wt d6 1|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:174 282993,Sample 0256 001 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_001_FR_NSP_TR1_SL1_S13_L001_R1_001-pooled.fastq.gz 0256_001_FR_NSP_TR1_SL1_S13_L001_R2_001-pooled.fastq.gz,fastq fastq,2739613002.0,26858951.0,ena RUN TAB 09 11 2022 11:52:59:174 282994,0:51 1:51,A:716316048;C:647929778;G:649844718;T:725429229;N:93229,51,51,,,716316048,647929778,649844718,725429229,93229,ERX9997130,ERS13672455,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11091,ERR10476812,ERX9997155,ERS13672480,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day13,SAMEA111562624,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d13 1|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star pos d13 1|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:185 283043,Sample 0256 081 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_081_FR_NSP_TR2_SL1_S12_L001_R1_001-pooled.fastq.gz 0256_081_FR_NSP_TR2_SL1_S12_L001_R2_001-pooled.fastq.gz,fastq fastq,2976324300.0,29179650.0,ena RUN TAB 09 11 2022 11:52:59:185 283044,0:51 1:51,A:831817738;C:637392987;G:662609648;T:844470228;N:33699,51,51,,,831817738,637392987,662609648,844470228,33699,ERX9997155,ERS13672480,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System
11092,ERR10476800,ERX9997143,ERS13672468,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 3 brains,wildtype whole brain day120,SAMEA111562612,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d120 4|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:tu wt d120 4|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:180 283019,Sample 0256 019 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_019_FR_NSP_TR1_SL1_S30_L001_R1_001-pooled.fastq.gz 0256_019_FR_NSP_TR1_SL1_S30_L001_R2_001-pooled.fastq.gz,fastq fastq,2911008498.0,28539299.0,ena RUN TAB 09 11 2022 11:52:59:180 283020,0:51 1:51,A:795092837;C:651247701;G:672040156;T:792529007;N:98797,51,51,,,795092837,651247701,672040156,792529007,98797,ERX9997143,ERS13672468,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System
11093,ERR10476840,ERX9997183,ERS13672508,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 3 brains,star:bPAC / whole brain day120,SAMEA111562652,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d120 4|collection date:2019 12|common name:zebrafish|dev stage:120 dpf|geographic location country and/or sea:Germany|sample name:star neg d120 4|scientific name:Danio rerio|sex:female|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:197 283099,Sample 0256 119 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_119_FR_NSP_TR1_SL1_S48_L001_R1_001-pooled.fastq.gz 0256_119_FR_NSP_TR1_SL1_S48_L001_R2_001-pooled.fastq.gz,fastq fastq,3396382740.0,33297870.0,ena RUN TAB 09 11 2022 11:52:59:197 283100,0:51 1:51,A:937800414;C:753433067;G:760774470;T:944336000;N:38789,51,51,,,937800414,753433067,760774470,944336000,38789,ERX9997183,ERS13672508,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Adult,Adult,Brain,Nervous System