rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
101,DRR189403,DRX179868,DRS200418,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 3,SAMD00182246,,sample name:CSUS Tel 30 2w memory 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182246,DRX179868,CSUS Tel 30 2w memory 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182246,,,,2791119960.0,77531110.0,DRR189403,0:36,A:685050951;C:641519684;G:664655385;T:799677669;N:216271,36,,,,685050951,641519684,664655385,799677669,216271,DRX179868,DRS200418,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89653,,0.1773,,0.70725,,0.49873,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System
102,DRR189402,DRX179867,DRS200417,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 2,SAMD00182245,,sample name:CSUS Tel 30 2w memory 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182245,DRX179867,CSUS Tel 30 2w memory 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182245,,,,1505449224.0,41818034.0,DRR189402,0:36,A:369895057;C:346914787;G:358624651;T:429897489;N:117240,36,,,,369895057,346914787,358624651,429897489,117240,DRX179867,DRS200417,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89514,,0.17677,,0.7025,,0.49571,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System
103,DRR189401,DRX179866,DRS200416,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 1,SAMD00182244,,sample name:CSUS Tel 30 2w memory 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182244,DRX179866,CSUS Tel 30 2w memory 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182244,,,,2088507024.0,58014084.0,DRR189401,0:36,A:516255405;C:478036869;G:496415722;T:597637091;N:161937,36,,,,516255405,478036869,496415722,597637091,161937,DRX179866,DRS200416,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89533,,0.18553,,0.70981,,0.49554,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System
104,DRR189400,DRX179865,DRS200401,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 3,SAMD00182243,,sample name:CSUS Tel 30 1d memory 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182243,DRX179865,CSUS Tel 30 1d memory 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182243,,,,1340808120.0,37244670.0,DRR189400,0:36,A:330513975;C:306935584;G:320089399;T:383165274;N:103888,36,,,,330513975,306935584,320089399,383165274,103888,DRX179865,DRS200401,DRA008864,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89272,,0.19494,,0.70335,,0.49456,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
105,DRR189399,DRX179864,DRS200400,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 2,SAMD00182242,,sample name:CSUS Tel 30 1d memory 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182242,DRX179864,CSUS Tel 30 1d memory 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182242,,,,1279740096.0,35548336.0,DRR189399,0:36,A:314850837;C:294188093;G:304654881;T:365946483;N:99802,36,,,,314850837,294188093,304654881,365946483,99802,DRX179864,DRS200400,DRA008864,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89485,,0.18205,,0.70593,,0.49333,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
106,DRR189398,DRX179863,DRS200399,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 1,SAMD00182241,,sample name:CSUS Tel 30 1d memory 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182241,DRX179863,CSUS Tel 30 1d memory 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182241,,,,3012353100.0,83676475.0,DRR189398,0:36,A:740795057;C:693120664;G:717419185;T:860784745;N:233449,36,,,,740795057,693120664,717419185,860784745,233449,DRX179863,DRS200399,DRA008864,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89676,,0.1791,,0.70569,,0.49835,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
107,DRR189397,DRX179862,DRS200428,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post exposure to the conditioning tank 3,SAMD00182240,,sample name:Cont Tel 60 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182240,DRX179862,Cont Tel 60 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182240,,,,5590147750.0,111802955.0,DRR189397,0:50,A:1365131560;C:1269631295;G:1366791321;T:1588422020;N:171554,50,,,,1365131560,1269631295,1366791321,1588422020,171554,DRX179862,DRS200428,DRA008863,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89334,,0.16354,,0.7011,,0.49383,,50,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
108,DRR189396,DRX179861,DRS200427,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post exposure to the conditioning tank 2,SAMD00182239,,sample name:Cont Tel 60 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182239,DRX179861,Cont Tel 60 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182239,,,,6429019650.0,128580393.0,DRR189396,0:50,A:1570141412;C:1457539089;G:1572785681;T:1828358007;N:195461,50,,,,1570141412,1457539089,1572785681,1828358007,195461,DRX179861,DRS200427,DRA008863,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89595,,0.16204,,0.70743,,0.49805,,50,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
109,DRR189395,DRX179860,DRS200426,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post exposure to the conditioning tank 1,SAMD00182238,,sample name:Cont Tel 60 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182238,DRX179860,Cont Tel 60 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182238,,,,6644185850.0,132883717.0,DRR189395,0:50,A:1637322491;C:1498943556;G:1616212676;T:1891505597;N:201530,50,,,,1637322491,1498943556,1616212676,1891505597,201530,DRX179860,DRS200426,DRA008863,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89573,,0.16421,,0.7037,,0.49805,,50,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
110,DRR189394,DRX179859,DRS200407,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post light stimulation in the conditioning tank 3,SAMD00182237,,sample name:CS Tel 60 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182237,DRX179859,CS Tel 60 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182237,,,,1387078956.0,38529971.0,DRR189394,0:36,A:333990578;C:315361737;G:337599372;T:400057968;N:69301,36,,,,333990578,315361737,337599372,400057968,69301,DRX179859,DRS200407,DRA008862,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88133,,0.18418,,0.70747,,0.4971,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
111,DRR189393,DRX179858,DRS200406,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post light stimulation in the conditioning tank 2,SAMD00182236,,sample name:CS Tel 60 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182236,DRX179858,CS Tel 60 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182236,,,,537029424.0,14917484.0,DRR189393,0:36,A:127421487;C:122494467;G:132164579;T:154921150;N:27741,36,,,,127421487,122494467,132164579,154921150,27741,DRX179858,DRS200406,DRA008862,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88264,,0.17793,,0.7083,,0.49123,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
112,DRR189392,DRX179857,DRS200405,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post light stimulation in the conditioning tank 1,SAMD00182235,,sample name:CS Tel 60 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182235,DRX179857,CS Tel 60 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182235,,,,3163663656.0,87879546.0,DRR189392,0:36,A:766146692;C:714108562;G:771785405;T:911468887;N:154110,36,,,,766146692,714108562,771785405,911468887,154110,DRX179857,DRS200405,DRA008862,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88114,,0.1803,,0.7052,,0.4917,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
113,DRR189391,DRX179856,DRS200404,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post electrical shock delivery in the conditioning tank 3,SAMD00182234,,sample name:US Tel 60 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182234,DRX179856,US Tel 60 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182234,,,,1535884704.0,42663464.0,DRR189391,0:36,A:367444721;C:350978310;G:375524359;T:441859454;N:77860,36,,,,367444721,350978310,375524359,441859454,77860,DRX179856,DRS200404,DRA008861,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88103,,0.1788,,0.7082,,0.49462,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
114,DRR189390,DRX179855,DRS200403,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post electrical shock delivery in the conditioning tank 2,SAMD00182233,,sample name:US Tel 60 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182233,DRX179855,US Tel 60 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182233,,,,737309916.0,20480831.0,DRR189390,0:36,A:176982713;C:168284406;G:179343270;T:212662841;N:36686,36,,,,176982713,168284406,179343270,212662841,36686,DRX179855,DRS200403,DRA008861,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.87995,,0.18515,,0.70816,,0.49393,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
115,DRR189389,DRX179854,DRS200402,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post electrical shock delivery in the conditioning tank 1,SAMD00182232,,sample name:US Tel 60 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182232,DRX179854,US Tel 60 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182232,,,,595837404.0,16551039.0,DRR189389,0:36,A:143956004;C:134783754;G:145443580;T:171624939;N:29127,36,,,,143956004,134783754,145443580,171624939,29127,DRX179854,DRS200402,DRA008861,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.87682,,0.18328,,0.70309,,0.49081,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
116,DRR189388,DRX179853,DRS200452,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 3,SAMD00182231,,sample name:CSUS Tel 60 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182231,DRX179853,CSUS Tel 60 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182231,,,,608050044.0,16890279.0,DRR189388,0:36,A:145025778;C:137906052;G:149542672;T:175545338;N:30204,36,,,,145025778,137906052,149542672,175545338,30204,DRX179853,DRS200452,DRA008860,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.87983,,0.18602,,0.70445,,0.49195,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
117,DRR189387,DRX179852,DRS200451,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 2,SAMD00182230,,sample name:CSUS Tel 60 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182230,DRX179852,CSUS Tel 60 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182230,,,,777589452.0,21599707.0,DRR189387,0:36,A:185463269;C:177742981;G:190443063;T:223900096;N:40043,36,,,,185463269,177742981,190443063,223900096,40043,DRX179852,DRS200451,DRA008860,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88331,,0.17918,,0.70516,,0.48956,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
118,DRR189386,DRX179851,DRS200450,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish  60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 1,SAMD00182229,,sample name:CSUS Tel 60 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182229,DRX179851,CSUS Tel 60 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182229,,,,2738622348.0,76072843.0,DRR189386,0:36,A:662989485;C:622023190;G:663013857;T:790459930;N:135886,36,,,,662989485,622023190,663013857,790459930,135886,DRX179851,DRS200450,DRA008860,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.87743,,0.19164,,0.70025,,0.49073,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
119,DRR189385,DRX179850,DRS200435,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of EMX3  /  adult zebrafish 3,SAMD00182228,,sample name:Emx3     Adult Tel 3|genotype:Emx3 / |tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182228,DRX179850,Emx3 /  Adult Tel 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182228,,,,1323636552.0,36767682.0,DRR189385,0:36,A:309582925;C:310206951;G:325673647;T:378136039;N:36990,36,,,,309582925,310206951,325673647,378136039,36990,DRX179850,DRS200435,DRA008859,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.8932,,0.19244,,0.71334,,0.50591,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
120,DRR189384,DRX179849,DRS200434,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of EMX3  /  adult zebrafish 2,SAMD00182227,,sample name:Emx3     Adult Tel 2|genotype:Emx3 / |tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182227,DRX179849,Emx3 /  Adult Tel 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182227,,,,2994105168.0,83169588.0,DRR189384,0:36,A:709730692;C:691531995;G:727245229;T:865514929;N:82323,36,,,,709730692,691531995,727245229,865514929,82323,DRX179849,DRS200434,DRA008859,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.90382,,0.1826,,0.70197,,0.49719,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
121,DRR189383,DRX179848,DRS200433,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of EMX3  /  adult zebrafish 1,SAMD00182226,,sample name:Emx3     Adult Tel 1|genotype:Emx3 / |tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182226,DRX179848,Emx3 /  Adult Tel 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182226,,,,1151464896.0,31985136.0,DRR189383,0:36,A:272427216;C:266708394;G:280781178;T:331515997;N:32111,36,,,,272427216,266708394,280781178,331515997,32111,DRX179848,DRS200433,DRA008859,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89942,,0.17402,,0.70364,,0.49438,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
122,DRR189382,DRX179847,DRS200421,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of wild type adult zebrafish 3,SAMD00182225,,sample name:WT Adult Tel 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182225,DRX179847,WT Adult Tel 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182225,,,,2837488824.0,78819134.0,DRR189382,0:36,A:678395895;C:655098137;G:687520693;T:816395518;N:78581,36,,,,678395895,655098137,687520693,816395518,78581,DRX179847,DRS200421,DRA008858,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89483,,0.18623,,0.70544,,0.49839,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
123,DRR189381,DRX179846,DRS200420,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of wild type adult zebrafish 2,SAMD00182224,,sample name:WT Adult Tel 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182224,DRX179846,WT Adult Tel 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182224,,,,1456800264.0,40466674.0,DRR189381,0:36,A:345320431;C:336838149;G:356264798;T:418336552;N:40334,36,,,,345320431,336838149,356264798,418336552,40334,DRX179846,DRS200420,DRA008858,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89496,,0.18691,,0.70802,,0.49721,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
124,DRR189380,DRX179845,DRS200419,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of wild type adult zebrafish 1,SAMD00182223,,sample name:WT Adult Tel 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182223,DRX179845,WT Adult Tel 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182223,,,,2533282452.0,70368957.0,DRR189380,0:36,A:602595083;C:585466052;G:616064286;T:729086926;N:70105,36,,,,602595083,585466052,616064286,729086926,70105,DRX179845,DRS200419,DRA008858,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89412,,0.19005,,0.70816,,0.49843,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
5795,ERR1698352,ERX1767860,ERS1417534,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 012 up 058 12,SAMEA4518355,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518355|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 012 up 058 12|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 012 up 058 12|sex:male,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 012 up 058 12 s,IonXpressRNA 012 up 058 12 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_012_up_058_12.fastq.gz,fastq,2535299006.0,33956077.0,E MTAB 5173:IonXpressRNA 012 up 058 12,0:74.66,A:719061985;C:581906269;G:579932234;T:654398518;N:0,74,,,,719061985,581906269,579932234,654398518,0,ERX1767860,ERS1417534,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.7343,,0.34119,,0.7359,,0.48406,,66,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5796,ERR1698351,ERX1767859,ERS1417533,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 011 up 058 11,SAMEA4518354,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518354|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 011 up 058 11|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 011 up 058 11|sex:male,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 011 up 058 11 s,IonXpressRNA 011 up 058 11 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_011_up_058_11.fastq.gz,fastq,3400774471.0,38709756.0,E MTAB 5173:IonXpressRNA 011 up 058 11,0:87.85,A:948991439;C:793389977;G:792539547;T:865853508;N:0,87,,,,948991439,793389977,792539547,865853508,0,ERX1767859,ERS1417533,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.63968,,0.27033,,0.75213,,0.47966,,139,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5797,ERR1698350,ERX1767858,ERS1417532,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 010 up 058 10,SAMEA4518353,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518353|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 010 up 058 10|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 010 up 058 10|sex:male,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 010 up 058 10 s,IonXpressRNA 010 up 058 10 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_010_up_058_10.fastq.gz,fastq,3502667998.0,39204781.0,E MTAB 5173:IonXpressRNA 010 up 058 10,0:89.34,A:976977303;C:803330255;G:808731995;T:913628445;N:0,89,,,,976977303,803330255,808731995,913628445,0,ERX1767858,ERS1417532,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.65846,,0.2815,,0.74028,,0.47784,,86,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5798,ERR1698349,ERX1767857,ERS1417531,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 009 up 058 9,SAMEA4518352,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518352|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 009 up 058 9|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 009 up 058 9|sex:male,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 009 up 058 9 s,IonXpressRNA 009 up 058 9 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: male:sex|Experimental Factor: n1:compound,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_009_up_058_9.fastq.gz,fastq,3575088264.0,39745481.0,E MTAB 5173:IonXpressRNA 009 up 058 9,0:89.95,A:1011474126;C:809896078;G:807471974;T:946246086;N:0,89,,,,1011474126,809896078,807471974,946246086,0,ERX1767857,ERS1417531,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.65266,,0.30274,,0.75286,,0.48527,,94,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5799,ERR1698348,ERX1767856,ERS1417530,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 008 up 058 8,SAMEA4518351,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518351|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 008 up 058 8|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 008 up 058 8|sex:male,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 008 up 058 8 s,IonXpressRNA 008 up 058 8 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: male:sex|Experimental Factor: n1:compound,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_008_up_058_8.fastq.gz,fastq,3259710114.0,38530802.0,E MTAB 5173:IonXpressRNA 008 up 058 8,0:84.60,A:916053004;C:755694888;G:751125332;T:836836890;N:0,84,,,,916053004,755694888,751125332,836836890,0,ERX1767856,ERS1417530,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.70995,,0.31365,,0.74781,,0.48846,,61,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5800,ERR1698347,ERX1767855,ERS1417529,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 007 up 058 7,SAMEA4518350,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518350|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 007 up 058 7|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 007 up 058 7|sex:male,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 007 up 058 7 s,IonXpressRNA 007 up 058 7 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: male:sex|Experimental Factor: n1:compound,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_007_up_058_7.fastq.gz,fastq,2307842164.0,28984666.0,E MTAB 5173:IonXpressRNA 007 up 058 7,0:79.62,A:634041732;C:548565089;G:545418985;T:579816358;N:0,79,,,,634041732,548565089,545418985,579816358,0,ERX1767855,ERS1417529,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.67542,,0.2817,,0.74168,,0.47224,,37,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5801,ERR1698346,ERX1767854,ERS1417528,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 006 up 058 6,SAMEA4518349,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518349|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 006 up 058 6|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 006 up 058 6|sex:female,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 006 up 058 6 s,IonXpressRNA 006 up 058 6 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_006_up_058_6.fastq.gz,fastq,2239484857.0,27021234.0,E MTAB 5173:IonXpressRNA 006 up 058 6,0:82.88,A:637326624;C:506830056;G:503508207;T:591819970;N:0,82,,,,637326624,506830056,503508207,591819970,0,ERX1767854,ERS1417528,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.66441,,0.31904,,0.74905,,0.47154,,122,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5802,ERR1698345,ERX1767853,ERS1417527,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 005 up 058 5,SAMEA4518348,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518348|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 005 up 058 5|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 005 up 058 5|sex:female,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 005 up 058 5 s,IonXpressRNA 005 up 058 5 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_005_up_058_5.fastq.gz,fastq,2920608487.0,39573075.0,E MTAB 5173:IonXpressRNA 005 up 058 5,0:73.80,A:814851327;C:678116994;G:691911192;T:735728974;N:0,73,,,,814851327,678116994,691911192,735728974,0,ERX1767853,ERS1417527,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.60005,,0.25542,,0.76481,,0.4886,,12,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5803,ERR1698344,ERX1767852,ERS1417526,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 004 up 058 4,SAMEA4518347,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518347|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 004 up 058 4|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 004 up 058 4|sex:female,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 004 up 058 4 s,IonXpressRNA 004 up 058 4 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_004_up_058_4.fastq.gz,fastq,2989727396.0,37953454.0,E MTAB 5173:IonXpressRNA 004 up 058 4,0:78.77,A:802963810;C:722434772;G:742850530;T:721478284;N:0,78,,,,802963810,722434772,742850530,721478284,0,ERX1767852,ERS1417526,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.76402,,0.32503,,0.74241,,0.4815,,128,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5804,ERR1698343,ERX1767851,ERS1417525,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 003 up 058 3,SAMEA4518346,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518346|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 003 up 058 3|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 003 up 058 3|sex:female,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 003 up 058 3 s,IonXpressRNA 003 up 058 3 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: female:sex|Experimental Factor: n1:compound,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_003_up_058_3.fastq.gz,fastq,4068383115.0,41996847.0,E MTAB 5173:IonXpressRNA 003 up 058 3,0:96.87,A:1133167706;C:944306379;G:948626944;T:1042282086;N:0,96,,,,1133167706,944306379,948626944,1042282086,0,ERX1767851,ERS1417525,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.54804,,0.22334,,0.76353,,0.49163,,93,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5805,ERR1698342,ERX1767850,ERS1417524,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 002 up 058 2,SAMEA4518345,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518345|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 002 up 058 2|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 002 up 058 2|sex:female,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 002 up 058 2 s,IonXpressRNA 002 up 058 2 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: female:sex|Experimental Factor: n1:compound,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_002_up_058_2.fastq.gz,fastq,2679963893.0,33552723.0,E MTAB 5173:IonXpressRNA 002 up 058 2,0:79.87,A:709092431;C:635329399;G:670478210;T:665063853;N:0,79,,,,709092431,635329399,670478210,665063853,0,ERX1767850,ERS1417524,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.43283,,0.18649,,0.81178,,0.52885,,116,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
5806,ERR1698341,ERX1767849,ERS1417523,ERP018188,PRJEB16335,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol,E-MTAB-5173,Transcriptome Analysis,17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173,,Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,IonXpressRNA 001 up 058 1,SAMEA4518344,"School of Natural Science, Technology and Environmental Studies Sodertorn University",ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518344|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 001 up 058 1|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 001 up 058 1|sex:female,,,,,,,,,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,E MTAB 5173:IonXpressRNA 001 up 058 1 s,IonXpressRNA 001 up 058 1 s,RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA,Experimental Factor: female:sex|Experimental Factor: n1:compound,RNA-Seq,TRANSCRIPTOMIC,RANDOM,SINGLE,ION_TORRENT,Ion Torrent Proton,,ERP018188,Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol,ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16,IonXpressRNA_001_up_058_1.fastq.gz,fastq,3358902499.0,39857024.0,E MTAB 5173:IonXpressRNA 001 up 058 1,0:84.27,A:933405205;C:784111716;G:780404241;T:860981337;N:0,84,,,,933405205,784111716,780404241,860981337,0,ERX1767849,ERS1417523,ERA739176,"School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive","School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive",1,0.71789,,0.33159,,0.76619,,0.514,,56,,B,,usable mapping rate,ion_torrent,ion_torrent,full_length,random_priming,ribozero,bulk,unknown,unknown,,Sweden,2016-10-19,Pharyngula,Embryo,Brain,Nervous System
10237,ERR7131169,ERX6698608,ERS8070397,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F8 Nega,SAMEA10418613,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 Nega s,F8 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F22-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L006_R1_001.fastq.gz,fastq,658093749.0,12903799.0,E MTAB 11083:2874F22 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L006,0:51 1:0,A:173355262;C:152464129;G:147489024;T:184739885;N:45449,51,0,,,173355262,152464129,147489024,184739885,45449,ERX6698608,ERS8070397,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78328,,0.15147,,0.71289,,0.53671,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10238,ERR7131170,ERX6698608,ERS8070397,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F8 Nega,SAMEA10418613,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 Nega s,F8 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F22-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L007_R1_001.fastq.gz,fastq,661103769.0,12962819.0,E MTAB 11083:2874F22 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L007,0:51 1:0,A:174223249;C:153223284;G:148276805;T:185335092;N:45339,51,0,,,174223249,153223284,148276805,185335092,45339,ERX6698608,ERS8070397,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78539,,0.15054,,0.70897,,0.5322,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10239,ERR7131167,ERX6698607,ERS8070396,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F8 mCherry GFP,SAMEA10418612,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry GFP s,F8 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F24-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L006_R1_001.fastq.gz,fastq,693183636.0,13591836.0,E MTAB 11083:2874F24 1 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L006,0:51 1:0,A:183977687;C:159275153;G:153149826;T:196732022;N:48948,51,0,,,183977687,159275153,153149826,196732022,48948,ERX6698607,ERS8070396,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68942,,0.15593,,0.75828,,0.51858,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10240,ERR7131168,ERX6698607,ERS8070396,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F8 mCherry GFP,SAMEA10418612,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry GFP s,F8 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F24-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L007_R1_001.fastq.gz,fastq,696648678.0,13659778.0,E MTAB 11083:2874F24 2 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L007,0:51 1:0,A:184971832;C:160126524;G:154012023;T:197490098;N:48201,51,0,,,184971832,160126524,154012023,197490098,48201,ERX6698607,ERS8070396,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.6906,,0.15634,,0.75909,,0.51346,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10241,ERR7131165,ERX6698606,ERS8070395,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F8 mCherry,SAMEA10418611,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry s,F8 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F23-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L006_R1_001.fastq.gz,fastq,667760646.0,13093346.0,E MTAB 11083:2874F23 1 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L006,0:51 1:0,A:179948739;C:150541918;G:145038845;T:192184092;N:47052,51,0,,,179948739,150541918,145038845,192184092,47052,ERX6698606,ERS8070395,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71661,,0.19774,,0.74576,,0.52117,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10242,ERR7131166,ERX6698606,ERS8070395,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F8 mCherry,SAMEA10418611,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry s,F8 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F23-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L007_R1_001.fastq.gz,fastq,670970484.0,13156284.0,E MTAB 11083:2874F23 2 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L007,0:51 1:0,A:180906557;C:151329492;G:145816347;T:192872804;N:45284,51,0,,,180906557,151329492,145816347,192872804,45284,ERX6698606,ERS8070395,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71682,,0.19902,,0.74517,,0.52618,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10243,ERR7131163,ERX6698605,ERS8070394,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F7 Nega,SAMEA10418610,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 Nega s,F7 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F19-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L006_R1_001.fastq.gz,fastq,652205238.0,12788338.0,E MTAB 11083:2874F19 1 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L006,0:51 1:0,A:173730405;C:149044598;G:145436777;T:183946928;N:46530,51,0,,,173730405,149044598,145436777,183946928,46530,ERX6698605,ERS8070394,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80595,,0.17309,,0.71003,,0.53696,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10244,ERR7131164,ERX6698605,ERS8070394,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F7 Nega,SAMEA10418610,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 Nega s,F7 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F19-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L007_R1_001.fastq.gz,fastq,655593423.0,12854773.0,E MTAB 11083:2874F19 2 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L007,0:51 1:0,A:174715119;C:149844974;G:146250446;T:184737535;N:45349,51,0,,,174715119,149844974,146250446,184737535,45349,ERX6698605,ERS8070394,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80585,,0.17497,,0.71078,,0.53767,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10245,ERR7131161,ERX6698604,ERS8070393,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F7 mCherry GFP,SAMEA10418609,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry GFP s,F7 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F21-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L006_R1_001.fastq.gz,fastq,661067151.0,12962101.0,E MTAB 11083:2874F21 1 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L006,0:51 1:0,A:168281419;C:157701209;G:153487197;T:181550252;N:47074,51,0,,,168281419,157701209,153487197,181550252,47074,ERX6698604,ERS8070393,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.39074,,0.11592,,0.82615,,0.5252,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10246,ERR7131162,ERX6698604,ERS8070393,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F7 mCherry GFP,SAMEA10418609,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry GFP s,F7 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F21-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L007_R1_001.fastq.gz,fastq,665709018.0,13053118.0,E MTAB 11083:2874F21 2 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L007,0:51 1:0,A:169539998;C:158884603;G:154624803;T:182614264;N:45350,51,0,,,169539998,158884603,154624803,182614264,45350,ERX6698604,ERS8070393,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.38966,,0.1152,,0.8258,,0.53305,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10247,ERR7131159,ERX6698603,ERS8070392,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F7 mCherry,SAMEA10418608,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry s,F7 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F20-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L006_R1_001.fastq.gz,fastq,657813402.0,12898302.0,E MTAB 11083:2874F20 1 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L006,0:51 1:0,A:175873489;C:149476129;G:143987770;T:188429588;N:46426,51,0,,,175873489,149476129,143987770,188429588,46426,ERX6698603,ERS8070392,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70507,,0.2048,,0.75923,,0.52828,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10248,ERR7131160,ERX6698603,ERS8070392,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F7 mCherry,SAMEA10418608,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry s,F7 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F20-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L007_R1_001.fastq.gz,fastq,660303426.0,12947126.0,E MTAB 11083:2874F20 2 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L007,0:51 1:0,A:176607119;C:150065781;G:144613110;T:188972809;N:44607,51,0,,,176607119,150065781,144613110,188972809,44607,ERX6698603,ERS8070392,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70705,,0.20314,,0.75852,,0.52949,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10249,ERR7131157,ERX6698602,ERS8070391,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F6 Nega,SAMEA10418607,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 Nega s,F6 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F16-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L006_R1_001.fastq.gz,fastq,669627450.0,13129950.0,E MTAB 11083:2874F16 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L006,0:51 1:0,A:177483937;C:154097051;G:148063012;T:189935690;N:47760,51,0,,,177483937,154097051,148063012,189935690,47760,ERX6698602,ERS8070391,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.77183,,0.16001,,0.71467,,0.53352,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10250,ERR7131158,ERX6698602,ERS8070391,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F6 Nega,SAMEA10418607,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 Nega s,F6 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F16-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L007_R1_001.fastq.gz,fastq,671615073.0,13168923.0,E MTAB 11083:2874F16 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L007,0:51 1:0,A:178109157;C:154619024;G:148595207;T:190246469;N:45216,51,0,,,178109157,154619024,148595207,190246469,45216,ERX6698602,ERS8070391,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.77284,,0.15963,,0.71569,,0.53038,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10251,ERR7131155,ERX6698601,ERS8070390,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F6 mCherry GFP,SAMEA10418606,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry GFP s,F6 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F18-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L006_R1_001.fastq.gz,fastq,667663542.0,13091442.0,E MTAB 11083:2874F18 1 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L006,0:51 1:0,A:177646483;C:152747528;G:146388226;T:190833951;N:47354,51,0,,,177646483,152747528,146388226,190833951,47354,ERX6698601,ERS8070390,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67964,,0.18123,,0.77193,,0.53313,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10252,ERR7131156,ERX6698601,ERS8070390,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F6 mCherry GFP,SAMEA10418606,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry GFP s,F6 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F18-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L007_R1_001.fastq.gz,fastq,670170141.0,13140591.0,E MTAB 11083:2874F18 2 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L007,0:51 1:0,A:178425395;C:153363855;G:147033779;T:191300976;N:46136,51,0,,,178425395,153363855,147033779,191300976,46136,ERX6698601,ERS8070390,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67922,,0.18101,,0.77141,,0.53601,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10253,ERR7131153,ERX6698600,ERS8070389,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F6 mCherry,SAMEA10418605,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry s,F6 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F17-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L006_R1_001.fastq.gz,fastq,677617008.0,13286608.0,E MTAB 11083:2874F17 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L006,0:51 1:0,A:181785899;C:152797687;G:147070676;T:195914808;N:47938,51,0,,,181785899,152797687,147070676,195914808,47938,ERX6698600,ERS8070389,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68298,,0.1953,,0.76292,,0.5395,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10254,ERR7131154,ERX6698600,ERS8070389,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F6 mCherry,SAMEA10418605,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry s,F6 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F17-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L007_R1_001.fastq.gz,fastq,679922973.0,13331823.0,E MTAB 11083:2874F17 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L007,0:51 1:0,A:182567819;C:153380607;G:147589890;T:196337722;N:46935,51,0,,,182567819,153380607,147589890,196337722,46935,ERX6698600,ERS8070389,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68391,,0.19726,,0.76299,,0.54099,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10255,ERR7131151,ERX6698599,ERS8070388,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F5 Nega,SAMEA10418604,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 Nega s,F5 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F13-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L006_R1_001.fastq.gz,fastq,620316315.0,12163065.0,E MTAB 11083:2874F13 1 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L006,0:51 1:0,A:165967355;C:141072216;G:136931219;T:176301963;N:43562,51,0,,,165967355,141072216,136931219,176301963,43562,ERX6698599,ERS8070388,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78924,,0.17457,,0.71934,,0.53563,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10256,ERR7131152,ERX6698599,ERS8070388,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F5 Nega,SAMEA10418604,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 Nega s,F5 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F13-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L007_R1_001.fastq.gz,fastq,623905338.0,12233438.0,E MTAB 11083:2874F13 2 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L007,0:51 1:0,A:166996253;C:141937637;G:137825206;T:177103504;N:42738,51,0,,,166996253,141937637,137825206,177103504,42738,ERX6698599,ERS8070388,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78975,,0.17387,,0.72153,,0.54149,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10257,ERR7131149,ERX6698598,ERS8070387,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F5 mCherry GFP,SAMEA10418603,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry GFP s,F5 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F15-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L006_R1_001.fastq.gz,fastq,670154841.0,13140291.0,E MTAB 11083:2874F15 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L006,0:51 1:0,A:182457480;C:149175456;G:144548173;T:193927470;N:46262,51,0,,,182457480,149175456,144548173,193927470,46262,ERX6698598,ERS8070387,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71875,,0.18671,,0.76047,,0.52398,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10258,ERR7131150,ERX6698598,ERS8070387,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F5 mCherry GFP,SAMEA10418603,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry GFP s,F5 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F15-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L007_R1_001.fastq.gz,fastq,674202048.0,13219648.0,E MTAB 11083:2874F15 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L007,0:51 1:0,A:183639511;C:150168226;G:145502071;T:194847755;N:44485,51,0,,,183639511,150168226,145502071,194847755,44485,ERX6698598,ERS8070387,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.72111,,0.18813,,0.76378,,0.5258,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10259,ERR7131147,ERX6698597,ERS8070386,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F5 mCherry,SAMEA10418602,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry s,F5 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F14-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L006_R1_001.fastq.gz,fastq,615458259.0,12067809.0,E MTAB 11083:2874F14 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L006,0:51 1:0,A:165108582;C:139178883;G:134070672;T:177059757;N:40365,51,0,,,165108582,139178883,134070672,177059757,40365,ERX6698597,ERS8070386,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69017,,0.1977,,0.77193,,0.53352,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10260,ERR7131148,ERX6698597,ERS8070386,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F5 mCherry,SAMEA10418602,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry s,F5 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F14-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L007_R1_001.fastq.gz,fastq,619061613.0,12138463.0,E MTAB 11083:2874F14 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L007,0:51 1:0,A:166167516;C:140058774;G:134972850;T:177822733;N:39740,51,0,,,166167516,140058774,134972850,177822733,39740,ERX6698597,ERS8070386,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69118,,0.20019,,0.7707,,0.52174,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10261,ERR7131145,ERX6698596,ERS8070385,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F4 Nega,SAMEA10418601,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 Nega s,F4 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F10-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L006_R1_001.fastq.gz,fastq,652536687.0,12794837.0,E MTAB 11083:2874F10 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L006,0:51 1:0,A:178033231;C:144661707;G:139876661;T:189921085;N:44003,51,0,,,178033231,144661707,139876661,189921085,44003,ERX6698596,ERS8070385,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.76201,,0.2508,,0.71299,,0.53379,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10262,ERR7131146,ERX6698596,ERS8070385,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F4 Nega,SAMEA10418601,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 Nega s,F4 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F10-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L007_R1_001.fastq.gz,fastq,655278957.0,12848607.0,E MTAB 11083:2874F10 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L007,0:51 1:0,A:178938002;C:145332613;G:140527756;T:190437189;N:43397,51,0,,,178938002,145332613,140527756,190437189,43397,ERX6698596,ERS8070385,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.76188,,0.25356,,0.71344,,0.53081,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10263,ERR7131143,ERX6698595,ERS8070384,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F4 mCherry GFP,SAMEA10418600,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry GFP s,F4 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F12-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L006_R1_001.fastq.gz,fastq,297921855.0,5841605.0,E MTAB 11083:2874F12 1 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L006,0:51 1:0,A:82400724;C:65517496;G:64603058;T:85384480;N:16097,51,0,,,82400724,65517496,64603058,85384480,16097,ERX6698595,ERS8070384,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.72496,,0.20794,,0.81223,,0.52529,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10264,ERR7131144,ERX6698595,ERS8070384,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F4 mCherry GFP,SAMEA10418600,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry GFP s,F4 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F12-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L007_R1_001.fastq.gz,fastq,309412971.0,6066921.0,E MTAB 11083:2874F12 2 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L007,0:51 1:0,A:85531271;C:68164437;G:67129209;T:88573210;N:14844,51,0,,,85531271,68164437,67129209,88573210,14844,ERX6698595,ERS8070384,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.7245,,0.20724,,0.80468,,0.52791,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10265,ERR7131141,ERX6698594,ERS8070383,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F4 mCherry,SAMEA10418599,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry s,F4 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F11-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L006_R1_001.fastq.gz,fastq,672319791.0,13182741.0,E MTAB 11083:2874F11 1 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L006,0:51 1:0,A:181508034;C:150928549;G:146124085;T:193711358;N:47765,51,0,,,181508034,150928549,146124085,193711358,47765,ERX6698594,ERS8070383,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71726,,0.19842,,0.74986,,0.53363,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10266,ERR7131142,ERX6698594,ERS8070383,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F4 mCherry,SAMEA10418599,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry s,F4 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F11-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L007_R1_001.fastq.gz,fastq,675486075.0,13244825.0,E MTAB 11083:2874F11 2 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L007,0:51 1:0,A:182462261;C:151688554;G:146902713;T:194385945;N:46602,51,0,,,182462261,151688554,146902713,194385945,46602,ERX6698594,ERS8070383,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71745,,0.19727,,0.74805,,0.53092,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10267,ERR7131139,ERX6698593,ERS8070382,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F3 Nega,SAMEA10418598,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 Nega s,F3 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F7-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L006_R1_001.fastq.gz,fastq,630093627.0,12354777.0,E MTAB 11083:2874F7 1 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L006,0:51 1:0,A:165132739;C:146179836;G:142796880;T:175940613;N:43559,51,0,,,165132739,146179836,142796880,175940613,43559,ERX6698593,ERS8070382,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81672,,0.15219,,0.71277,,0.52461,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10268,ERR7131140,ERX6698593,ERS8070382,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F3 Nega,SAMEA10418598,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 Nega s,F3 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F7-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L007_R1_001.fastq.gz,fastq,632305242.0,12398142.0,E MTAB 11083:2874F7 2 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L007,0:51 1:0,A:165832174;C:146751895;G:143384539;T:176293313;N:43321,51,0,,,165832174,146751895,143384539,176293313,43321,ERX6698593,ERS8070382,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81791,,0.15444,,0.7151,,0.52585,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10269,ERR7131137,ERX6698592,ERS8070381,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F3 mCherry GFP,SAMEA10418597,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry GFP s,F3 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F9-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L006_R1_001.fastq.gz,fastq,660379620.0,12948620.0,E MTAB 11083:2874F9 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L006,0:51 1:0,A:178482695;C:147417787;G:143797476;T:190635173;N:46489,51,0,,,178482695,147417787,143797476,190635173,46489,ERX6698592,ERS8070381,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.65635,,0.17778,,0.76899,,0.52957,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10270,ERR7131138,ERX6698592,ERS8070381,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F3 mCherry GFP,SAMEA10418597,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry GFP s,F3 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F9-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L007_R1_001.fastq.gz,fastq,662411307.0,12988457.0,E MTAB 11083:2874F9 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L007,0:51 1:0,A:179158323;C:147942300;G:144319925;T:190945370;N:45389,51,0,,,179158323,147942300,144319925,190945370,45389,ERX6698592,ERS8070381,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.65813,,0.17896,,0.77076,,0.52835,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10271,ERR7131135,ERX6698591,ERS8070380,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F3 mCherry,SAMEA10418596,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry s,F3 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F8-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L006_R1_001.fastq.gz,fastq,698608353.0,13698203.0,E MTAB 11083:2874F8 1 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L006,0:51 1:0,A:188718612;C:156814081;G:151125083;T:201901570;N:49007,51,0,,,188718612,156814081,151125083,201901570,49007,ERX6698591,ERS8070380,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70185,,0.21547,,0.75588,,0.53853,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10272,ERR7131136,ERX6698591,ERS8070380,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F3 mCherry,SAMEA10418596,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry s,F3 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F8-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L007_R1_001.fastq.gz,fastq,699869175.0,13722925.0,E MTAB 11083:2874F8 2 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L007,0:51 1:0,A:189192315;C:157176897;G:151498983;T:201952867;N:48113,51,0,,,189192315,157176897,151498983,201952867,48113,ERX6698591,ERS8070380,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70221,,0.21684,,0.75621,,0.53787,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10273,ERR7131133,ERX6698590,ERS8070379,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F2 Nega,SAMEA10418595,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 Nega s,F2 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F4-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L006_R1_001.fastq.gz,fastq,573812985.0,11251235.0,E MTAB 11083:2874F4 1 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L006,0:51 1:0,A:150716404;C:132203167;G:129732968;T:161120057;N:40389,51,0,,,150716404,132203167,129732968,161120057,40389,ERX6698590,ERS8070379,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81899,,0.17302,,0.72281,,0.54315,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10274,ERR7131134,ERX6698590,ERS8070379,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F2 Nega,SAMEA10418595,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 Nega s,F2 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F4-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L007_R1_001.fastq.gz,fastq,576184587.0,11297737.0,E MTAB 11083:2874F4 2 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L007,0:51 1:0,A:151387408;C:132788210;G:130392043;T:161577529;N:39397,51,0,,,151387408,132788210,130392043,161577529,39397,ERX6698590,ERS8070379,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81999,,0.17082,,0.72196,,0.54505,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10275,ERR7131131,ERX6698589,ERS8070378,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F2 mCherry GFP,SAMEA10418594,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry GFP s,F2 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F6-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L006_R1_001.fastq.gz,fastq,654811032.0,12839432.0,E MTAB 11083:2874F6 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L006,0:51 1:0,A:177403486;C:146202733;G:142207545;T:188951373;N:45895,51,0,,,177403486,146202733,142207545,188951373,45895,ERX6698589,ERS8070378,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67854,,0.21407,,0.76104,,0.52713,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10276,ERR7131132,ERX6698589,ERS8070378,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F2 mCherry GFP,SAMEA10418594,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry GFP s,F2 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F6-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L007_R1_001.fastq.gz,fastq,656911926.0,12880626.0,E MTAB 11083:2874F6 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L007,0:51 1:0,A:178119212;C:146730132;G:142725348;T:189293212;N:44022,51,0,,,178119212,146730132,142725348,189293212,44022,ERX6698589,ERS8070378,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68162,,0.21431,,0.761,,0.5071,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10277,ERR7131129,ERX6698588,ERS8070377,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F2 mCherry,SAMEA10418593,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry s,F2 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F5-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L006_R1_001.fastq.gz,fastq,607851558.0,11918658.0,E MTAB 11083:2874F5 1 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L006,0:51 1:0,A:164814138;C:136071648;G:132945950;T:173976881;N:42941,51,0,,,164814138,136071648,132945950,173976881,42941,ERX6698588,ERS8070377,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78967,,0.18905,,0.73241,,0.52347,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10278,ERR7131130,ERX6698588,ERS8070377,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F2 mCherry,SAMEA10418593,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry s,F2 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F5-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L007_R1_001.fastq.gz,fastq,610931805.0,11979055.0,E MTAB 11083:2874F5 2 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L007,0:51 1:0,A:165714775;C:136822925;G:133684294;T:174667814;N:41997,51,0,,,165714775,136822925,133684294,174667814,41997,ERX6698588,ERS8070377,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78869,,0.18885,,0.73156,,0.51691,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10279,ERR7131127,ERX6698587,ERS8070376,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F1 Nega,SAMEA10418592,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 Nega s,F1 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F1-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L006_R1_001.fastq.gz,fastq,619561872.0,12148272.0,E MTAB 11083:2874F1 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L006,0:51 1:0,A:164385545;C:141746313;G:138163219;T:175223323;N:43472,51,0,,,164385545,141746313,138163219,175223323,43472,ERX6698587,ERS8070376,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80258,,0.17703,,0.72614,,0.53483,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10280,ERR7131128,ERX6698587,ERS8070376,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F1 Nega,SAMEA10418592,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 Nega s,F1 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F1-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L007_R1_001.fastq.gz,fastq,621481971.0,12185921.0,E MTAB 11083:2874F1 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L007,0:51 1:0,A:165030774;C:142231469;G:138651078;T:175527080;N:41570,51,0,,,165030774,142231469,138651078,175527080,41570,ERX6698587,ERS8070376,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80248,,0.17699,,0.726,,0.53711,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10281,ERR7131125,ERX6698586,ERS8070375,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F1 mCherry GFP,SAMEA10418591,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry GFP s,F1 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F3-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L006_R1_001.fastq.gz,fastq,651189369.0,12768419.0,E MTAB 11083:2874F3 1 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L006,0:51 1:0,A:176398415;C:145659111;G:142137610;T:186948280;N:45953,51,0,,,176398415,145659111,142137610,186948280,45953,ERX6698586,ERS8070375,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69561,,0.18489,,0.75345,,0.52316,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10282,ERR7131126,ERX6698586,ERS8070375,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F1 mCherry GFP,SAMEA10418591,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry GFP s,F1 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F3-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L007_R1_001.fastq.gz,fastq,653398944.0,12811744.0,E MTAB 11083:2874F3 2 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L007,0:51 1:0,A:177139734;C:146171766;G:142707146;T:187335475;N:44823,51,0,,,177139734,146171766,142707146,187335475,44823,ERX6698586,ERS8070375,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69496,,0.18414,,0.75375,,0.52317,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10283,ERR7131123,ERX6698585,ERS8070374,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F1 mCherry,SAMEA10418590,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry s,F1 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F2-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L006_R1_001.fastq.gz,fastq,613574319.0,12030869.0,E MTAB 11083:2874F2 1 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L006,0:51 1:0,A:164891629;C:138401759;G:135818073;T:174419828;N:43030,51,0,,,164891629,138401759,135818073,174419828,43030,ERX6698585,ERS8070374,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.79499,,0.21674,,0.73359,,0.52207,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10284,ERR7131124,ERX6698585,ERS8070374,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,F1 mCherry,SAMEA10418590,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry s,F1 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F2-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L007_R1_001.fastq.gz,fastq,615666798.0,12071898.0,E MTAB 11083:2874F2 2 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L007,0:51 1:0,A:165536502;C:138952758;G:136375193;T:174760659;N:41686,51,0,,,165536502,138952758,136375193,174760659,41686,ERX6698585,ERS8070374,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.7941,,0.21645,,0.73494,,0.50761,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
28692,SRR26535343,SRX22238470,SRS19292731,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 2,GSM7866394,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 2,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866394,GSM7866394: EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 2; Danio rerio; RNA Seq,GSM7866394 r1,GSM7866394,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,CTRL_3.fastq.gz,fastq,7081948098.0,70118298.0,GSM7866394 r1,0:101,A:1894557984;C:1627945034;G:1550203852;T:2009224463;N:16765,101,,,,1894557984,1627945034,1550203852,2009224463,16765,SRX22238470,SRS19292731,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.94543,,0.14646,,0.68883,,0.49451,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28693,SRR26535344,SRX22238469,SRS19292730,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 1,GSM7866393,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 1,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866393,GSM7866393: EGFRviii  PI3KCAH1047R  mScarlet tumor negative control brain 1; Danio rerio; RNA Seq,GSM7866393 r1,GSM7866393,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,CTRL_2.fastq.gz,fastq,7840572329.0,77629429.0,GSM7866393 r1,0:101,A:2099360211;C:1783653297;G:1717681461;T:2239858766;N:18594,101,,,,2099360211,1783653297,1717681461,2239858766,18594,SRX22238469,SRS19292730,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.93984,,0.1663,,0.69033,,0.484,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28694,SRR26535345,SRX22238468,SRS19292729,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,KRAS PI3KCAH1047R  GFP tumor negative control brain,GSM7866392,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,KRAS PI3KCAH1047R  GFP tumor negative control brain,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866392,GSM7866392: KRAS PI3KCAH1047R  GFP tumor negative control brain; Danio rerio; RNA Seq,GSM7866392 r1,GSM7866392,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,CTRL_1.fastq.gz,fastq,10164914720.0,100642720.0,GSM7866392 r1,0:101,A:2650336113;C:2354113843;G:2276252979;T:2884188285;N:23500,101,,,,2650336113,2354113843,2276252979,2884188285,23500,SRX22238468,SRS19292729,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.94271,,0.10831,,0.68527,,0.50207,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28695,SRR26535346,SRX22238467,SRS19292728,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 3,GSM7866391,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 3,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866391,GSM7866391: EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 3; Danio rerio; RNA Seq,GSM7866391 r1,GSM7866391,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,EPS_3.fastq.gz,fastq,9796714170.0,96997170.0,GSM7866391 r1,0:101,A:2627522717;C:2223655583;G:2134761894;T:2810751194;N:22782,101,,,,2627522717,2223655583,2134761894,2810751194,22782,SRX22238467,SRS19292728,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.92543,,0.1876,,0.67308,,0.4886,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28696,SRR26535347,SRX22238466,SRS19292727,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 2,GSM7866390,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 2,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866390,GSM7866390: EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 2; Danio rerio; RNA Seq,GSM7866390 r1,GSM7866390,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,EPS_2.fastq.gz,fastq,7839595861.0,77619761.0,GSM7866390 r1,0:101,A:2093456023;C:1792947580;G:1718784421;T:2234389453;N:18384,101,,,,2093456023,1792947580,1718784421,2234389453,18384,SRX22238466,SRS19292727,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.93807,,0.14978,,0.68854,,0.47786,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28697,SRR26535348,SRX22238465,SRS19292726,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 1,GSM7866389,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 1,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866389,GSM7866389: EGFRviii  PI3KCAH1047R  mScarlet tumor positive whole brain 1; Danio rerio; RNA Seq,GSM7866389 r1,GSM7866389,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,EPS_1.fastq.gz,fastq,8403475528.0,83202728.0,GSM7866389 r1,0:101,A:2217465507;C:1940028683;G:1857031218;T:2388930530;N:19590,101,,,,2217465507,1940028683,1857031218,2388930530,19590,SRX22238465,SRS19292726,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.94596,,0.12916,,0.68619,,0.4957,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28698,SRR26535349,SRX22238464,SRS19292725,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,KRAS PI3KCAH1047R  GFP tumor positive whole brain 3,GSM7866388,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,KRAS PI3KCAH1047R  GFP tumor positive whole brain 3,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866388,GSM7866388: KRAS PI3KCAH1047R  GFP tumor positive whole brain 3; Danio rerio; RNA Seq,GSM7866388 r1,GSM7866388,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,KPG_3.fastq.gz,fastq,7609183450.0,75338450.0,GSM7866388 r1,0:101,A:2040241047;C:1721563382;G:1666400346;T:2180961056;N:17619,101,,,,2040241047,1721563382,1666400346,2180961056,17619,SRX22238464,SRS19292725,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.93555,,0.16895,,0.68199,,0.50032,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28699,SRR26535350,SRX22238463,SRS19292724,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,KRAS PI3KCAH1047R  GFP tumor positive whole brain 2,GSM7866387,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,KRAS PI3KCAH1047R  GFP tumor positive whole brain 2,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866387,GSM7866387: KRAS PI3KCAH1047R  GFP tumor positive whole brain 2; Danio rerio; RNA Seq,GSM7866387 r1,GSM7866387,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,KPG_2.fastq.gz,fastq,8446573339.0,83629439.0,GSM7866387 r1,0:101,A:2223763293;C:1944013929;G:1869828652;T:2408947564;N:19901,101,,,,2223763293,1944013929,1869828652,2408947564,19901,SRX22238463,SRS19292724,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.94943,,0.11539,,0.68428,,0.51019,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
28700,SRR26535351,SRX22238462,SRS19292723,SRP468564,PRJNA1032461,A syngeneic spontaneous zebrafish model oftp53 deficient  EGFRviii  and PI3KCAH1047R driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapsein vivo,GSE246295,Transcriptome Analysis,To build a patient relevantin vivomodel of human glioblastoma  we expressed common oncogenic variants including activated human EGFRviii or KRAS and PI3KCAH1047Runder the control of the radial glial specific promoterher4.1in syngeneictp53loss of function mutant zebrafish.Robust tumor formation was observed prior to 45 days of life  with a gene expression signature similar to human glioblastoma of the mesenchymal subtype  along with a strong inflammatory component. Within early stage tumor lesions  and in an intact and endogenous tumor microenvironment  we visualized infiltration of phagocytic cells  as well as internalization of tumor cells bympeg1.1:GFP+ microglia/macrophages  suggesting negative regulatory pressure by pro inflammatory cell types on tumor growth at early stages of glioblastoma initiationin vivo. Furthermore  CRISPR/Cas9 mediated gene targeting of master inflammatory transcription factorsirf7andirf8led to increased tumor formation in the primary context  while suppression of microglial/macrophage activity led to enhanced tumor cell engraftment following transplantation into otherwise immune competent zebrafish hosts. Altogether  we developed a genetically relevant model of aggressive human glioblastoma and harnessed the unique advantages of zebrafish including live imaging  high throughput genetic and chemical manipulations to highlight important tumor suppressive roles for the innate immune system on glioblastoma initiation  with important future significance for therapeutic discovery and optimizations. Overall design: To capture broad transcriptomic differences in tumor burdened brains versus normal brains  we performed bulk RNA sequencing and gene expression analysis of whole brains and sorted cells.  EGFRviii derived tumors were induced by microinjection of EGFRviii  PI3KCAH1047R  and mScarlet constructs defined as EPS. KRAS derived tumors were induced by microinjection of KRAS  PI3KCAH1047R  GFP constructs defined as KPG. Three whole brains positive for fluorescent tumors EPS 1 3  KPG 1 3  alongside three control brains from injected siblings who did not develop tumors CTRL 1 3  were dissected for each condition for bulk RNA sequencing. We also performed fluorescent activated cell sorting on pooled dissociated brains from the EPS condition  resulting in enrichment for mScarlet postive tumor cells CS Pos and mScarlet negative cells CS Neg for comparative analysis.,,pubmed:39052000,,KRAS PI3KCAH1047R  GFP tumor positive whole brain 1,GSM7866386,,source name:Whole Brain|strain:CG1 p53null|tissue:Whole Brain|geo loc name:missing|collection date:missing,KRAS PI3KCAH1047R  GFP tumor positive whole brain 1,Raw .fastq data was processed using Salmon quantification of transcripts for each sample. A “decoy aware” index was built with the Danio rerio transcriptome and genome using the GRCz11 assembly with a k mers length of 23  with entries manually added for GFP  mScarlet  EGFRviii  KRAS  and PI3KCAH1047R transcripts. Samples were then quantified with the following arguments:  r    seqBias    mp  3    validateMappings    rangeFactorizationBins 4. Differential gene expression was compared utilizing DESeq2 and GSEA. Assembly: GRCz11 Supplementary files format and content: Tab delimited .csv file including raw counts for all conditions.,Whole Brain,Fertilized embryos injected with either EPS or KPG construct mix,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer’s recommendations,Zebrafish were housed and reared following approved animal use protocol,strain:CG1 p53null|tissue:Whole Brain,GSM7866386,GSM7866386: KRAS PI3KCAH1047R  GFP tumor positive whole brain 1; Danio rerio; RNA Seq,GSM7866386 r1,GSM7866386,1,Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina  according to manufacturer's recommendations,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP468564,,loader:fastq load.py,KPG_1.fastq.gz,fastq,4326209053.0,42833753.0,GSM7866386 r1,0:101,A:1143856405;C:993993009;G:951829931;T:1236519669;N:10039,101,,,,1143856405,993993009,951829931,1236519669,10039,SRX22238462,SRS19292723,SRA1740017,"Hayes Lab, DSCB, Sickkids Research","Hayes Lab, DSCB, Sickkids Research",1,0.94443,,0.13057,,0.68503,,0.50149,,101,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Canada,2023-10-26,Undetermined,Embryo,Brain,Nervous System
29933,SRR27592958,SRX23261722,SRS20163687,SRP484215,PRJNA1065838,Diencephalic and Neuropeptidergic Dysfunction in Zebrafish with Autism Risk Mutations,GSE253405,Transcriptome Analysis,Hundreds of human mutations are linked to autism and related disorders  yet the functions of many of these mutated genes during vertebrate neural development are unclear. We generated 28 zebrafish mutants with presumptive protein truncating mutations or patient specific missense variants corresponding to autism risk alleles in 17 human genes. We observed baseline and stimulus driven behavioral changes at larval stages  as well as social behavior differences in lines tested as juveniles. Imaging whole brain activity revealed a near identical activity map for mutations in the unrelated genes kmt5b and hdlbpa  defined by increased activity mainly in the diencephalon. Truncating 7 of the 17 risk genes resulted in substantial brain size differences. Using RNA sequencing  we further defined molecular drivers of the observed phenotypes  identifying targetable disruptions in neuropeptide signaling  neuronal maturation  and cell proliferation. This multi modal screen has nominated brain regions  cell types  and molecular pathways that may contribute to autism susceptibility. Overall design: Dissected heads of larval zebrafish and brains of adult zebrafish mutants in genes that increase risk for autism  which were generated using CRISPR/Cas9 mutagenesis.,,,,arid1b  adult  rep15,GSM8020230,,source name:single dissected adult brain  male|tissue:single dissected adult brain  male|genotype:arid1b homozygous|geo loc name:missing|collection date:missing,arid1b  adult  rep15,Single end reads were aligned to GRCz11 release 104 using the Lawson Lab Zebrafish Transcriptome Annotation version 4.3.2 with STAR aligner 2.7.3a GCC 6.4.0 2.28. https://www.umassmed.edu/lawson lab/reagents/zebrafish transcriptome/ The raw counts files from STAR  in this record  were normalized using the rlog method in DESeq2 for subsequent published analysis. Assembly: GRCz11 Supplementary files format and content: Raw counts files from STAR; expected input for DESeq2.,single dissected adult brain  male,,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,tissue:single dissected adult brain  male|genotype:arid1b homozygous,GSM8020230,GSM8020230: arid1b  adult  rep15; Danio rerio; RNA Seq,GSM8020230 r1,GSM8020230,1,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP484215,,loader:fastq load.py,arid1b-adult-set2-hom_A8_S20_R1_001.fastq.gz,fastq,4265635010.0,42234010.0,GSM8020230 r1,0:101,A:1152569190;C:1002056793;G:969860357;T:1141141715;N:6955,101,,,,1152569190,1002056793,969860357,1141141715,6955,SRX23261722,SRS20163687,SRA1787174,UMass Chan Medical School,UMass Chan Medical School,,,,,,,,,,,,B,,usable mapping rate,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2024-01-16,Adult,Adult,Brain,Nervous System
29934,SRR27592959,SRX23261721,SRS20163686,SRP484215,PRJNA1065838,Diencephalic and Neuropeptidergic Dysfunction in Zebrafish with Autism Risk Mutations,GSE253405,Transcriptome Analysis,Hundreds of human mutations are linked to autism and related disorders  yet the functions of many of these mutated genes during vertebrate neural development are unclear. We generated 28 zebrafish mutants with presumptive protein truncating mutations or patient specific missense variants corresponding to autism risk alleles in 17 human genes. We observed baseline and stimulus driven behavioral changes at larval stages  as well as social behavior differences in lines tested as juveniles. Imaging whole brain activity revealed a near identical activity map for mutations in the unrelated genes kmt5b and hdlbpa  defined by increased activity mainly in the diencephalon. Truncating 7 of the 17 risk genes resulted in substantial brain size differences. Using RNA sequencing  we further defined molecular drivers of the observed phenotypes  identifying targetable disruptions in neuropeptide signaling  neuronal maturation  and cell proliferation. This multi modal screen has nominated brain regions  cell types  and molecular pathways that may contribute to autism susceptibility. Overall design: Dissected heads of larval zebrafish and brains of adult zebrafish mutants in genes that increase risk for autism  which were generated using CRISPR/Cas9 mutagenesis.,,,,arid1b  adult  rep14,GSM8020229,,source name:single dissected adult brain  male|tissue:single dissected adult brain  male|genotype:arid1b homozygous|geo loc name:missing|collection date:missing,arid1b  adult  rep14,Single end reads were aligned to GRCz11 release 104 using the Lawson Lab Zebrafish Transcriptome Annotation version 4.3.2 with STAR aligner 2.7.3a GCC 6.4.0 2.28. https://www.umassmed.edu/lawson lab/reagents/zebrafish transcriptome/ The raw counts files from STAR  in this record  were normalized using the rlog method in DESeq2 for subsequent published analysis. Assembly: GRCz11 Supplementary files format and content: Raw counts files from STAR; expected input for DESeq2.,single dissected adult brain  male,,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,tissue:single dissected adult brain  male|genotype:arid1b homozygous,GSM8020229,GSM8020229: arid1b  adult  rep14; Danio rerio; RNA Seq,GSM8020229 r1,GSM8020229,1,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP484215,,loader:fastq load.py,arid1b-adult-set2-hom_A6_S18_R1_001.fastq.gz,fastq,1422969608.0,14088808.0,GSM8020229 r1,0:101,A:389767872;C:327820009;G:327097969;T:378282090;N:1668,101,,,,389767872,327820009,327097969,378282090,1668,SRX23261721,SRS20163686,SRA1787174,UMass Chan Medical School,UMass Chan Medical School,,,,,,,,,,,,B,,usable mapping rate,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2024-01-16,Adult,Adult,Brain,Nervous System
29935,SRR27592960,SRX23261720,SRS20163685,SRP484215,PRJNA1065838,Diencephalic and Neuropeptidergic Dysfunction in Zebrafish with Autism Risk Mutations,GSE253405,Transcriptome Analysis,Hundreds of human mutations are linked to autism and related disorders  yet the functions of many of these mutated genes during vertebrate neural development are unclear. We generated 28 zebrafish mutants with presumptive protein truncating mutations or patient specific missense variants corresponding to autism risk alleles in 17 human genes. We observed baseline and stimulus driven behavioral changes at larval stages  as well as social behavior differences in lines tested as juveniles. Imaging whole brain activity revealed a near identical activity map for mutations in the unrelated genes kmt5b and hdlbpa  defined by increased activity mainly in the diencephalon. Truncating 7 of the 17 risk genes resulted in substantial brain size differences. Using RNA sequencing  we further defined molecular drivers of the observed phenotypes  identifying targetable disruptions in neuropeptide signaling  neuronal maturation  and cell proliferation. This multi modal screen has nominated brain regions  cell types  and molecular pathways that may contribute to autism susceptibility. Overall design: Dissected heads of larval zebrafish and brains of adult zebrafish mutants in genes that increase risk for autism  which were generated using CRISPR/Cas9 mutagenesis.,,,,arid1b  adult  rep13,GSM8020228,,source name:single dissected adult brain  male|tissue:single dissected adult brain  male|genotype:arid1b homozygous|geo loc name:missing|collection date:missing,arid1b  adult  rep13,Single end reads were aligned to GRCz11 release 104 using the Lawson Lab Zebrafish Transcriptome Annotation version 4.3.2 with STAR aligner 2.7.3a GCC 6.4.0 2.28. https://www.umassmed.edu/lawson lab/reagents/zebrafish transcriptome/ The raw counts files from STAR  in this record  were normalized using the rlog method in DESeq2 for subsequent published analysis. Assembly: GRCz11 Supplementary files format and content: Raw counts files from STAR; expected input for DESeq2.,single dissected adult brain  male,,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,tissue:single dissected adult brain  male|genotype:arid1b homozygous,GSM8020228,GSM8020228: arid1b  adult  rep13; Danio rerio; RNA Seq,GSM8020228 r1,GSM8020228,1,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP484215,,loader:fastq load.py,arid1b-adult-set2-hom_A4_S16_R1_001.fastq.gz,fastq,4331217239.0,42883339.0,GSM8020228 r1,0:101,A:1169654932;C:1016829715;G:986390612;T:1158335000;N:6980,101,,,,1169654932,1016829715,986390612,1158335000,6980,SRX23261720,SRS20163685,SRA1787174,UMass Chan Medical School,UMass Chan Medical School,,,,,,,,,,,,B,,usable mapping rate,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2024-01-16,Adult,Adult,Brain,Nervous System
29936,SRR27592961,SRX23261719,SRS20163684,SRP484215,PRJNA1065838,Diencephalic and Neuropeptidergic Dysfunction in Zebrafish with Autism Risk Mutations,GSE253405,Transcriptome Analysis,Hundreds of human mutations are linked to autism and related disorders  yet the functions of many of these mutated genes during vertebrate neural development are unclear. We generated 28 zebrafish mutants with presumptive protein truncating mutations or patient specific missense variants corresponding to autism risk alleles in 17 human genes. We observed baseline and stimulus driven behavioral changes at larval stages  as well as social behavior differences in lines tested as juveniles. Imaging whole brain activity revealed a near identical activity map for mutations in the unrelated genes kmt5b and hdlbpa  defined by increased activity mainly in the diencephalon. Truncating 7 of the 17 risk genes resulted in substantial brain size differences. Using RNA sequencing  we further defined molecular drivers of the observed phenotypes  identifying targetable disruptions in neuropeptide signaling  neuronal maturation  and cell proliferation. This multi modal screen has nominated brain regions  cell types  and molecular pathways that may contribute to autism susceptibility. Overall design: Dissected heads of larval zebrafish and brains of adult zebrafish mutants in genes that increase risk for autism  which were generated using CRISPR/Cas9 mutagenesis.,,,,arid1b  adult  rep12,GSM8020227,,source name:single dissected adult brain  male|tissue:single dissected adult brain  male|genotype:arid1b homozygous|geo loc name:missing|collection date:missing,arid1b  adult  rep12,Single end reads were aligned to GRCz11 release 104 using the Lawson Lab Zebrafish Transcriptome Annotation version 4.3.2 with STAR aligner 2.7.3a GCC 6.4.0 2.28. https://www.umassmed.edu/lawson lab/reagents/zebrafish transcriptome/ The raw counts files from STAR  in this record  were normalized using the rlog method in DESeq2 for subsequent published analysis. Assembly: GRCz11 Supplementary files format and content: Raw counts files from STAR; expected input for DESeq2.,single dissected adult brain  male,,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,tissue:single dissected adult brain  male|genotype:arid1b homozygous,GSM8020227,GSM8020227: arid1b  adult  rep12; Danio rerio; RNA Seq,GSM8020227 r1,GSM8020227,1,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP484215,,loader:fastq load.py,arid1b-adult-set2-hom_A2_S14_R1_001.fastq.gz,fastq,820969107.0,8128407.0,GSM8020227 r1,0:101,A:229523100;C:185511734;G:179068656;T:226864374;N:1243,101,,,,229523100,185511734,179068656,226864374,1243,SRX23261719,SRS20163684,SRA1787174,UMass Chan Medical School,UMass Chan Medical School,,,,,,,,,,,,B,,usable mapping rate,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2024-01-16,Adult,Adult,Brain,Nervous System
29937,SRR27592962,SRX23261718,SRS20163683,SRP484215,PRJNA1065838,Diencephalic and Neuropeptidergic Dysfunction in Zebrafish with Autism Risk Mutations,GSE253405,Transcriptome Analysis,Hundreds of human mutations are linked to autism and related disorders  yet the functions of many of these mutated genes during vertebrate neural development are unclear. We generated 28 zebrafish mutants with presumptive protein truncating mutations or patient specific missense variants corresponding to autism risk alleles in 17 human genes. We observed baseline and stimulus driven behavioral changes at larval stages  as well as social behavior differences in lines tested as juveniles. Imaging whole brain activity revealed a near identical activity map for mutations in the unrelated genes kmt5b and hdlbpa  defined by increased activity mainly in the diencephalon. Truncating 7 of the 17 risk genes resulted in substantial brain size differences. Using RNA sequencing  we further defined molecular drivers of the observed phenotypes  identifying targetable disruptions in neuropeptide signaling  neuronal maturation  and cell proliferation. This multi modal screen has nominated brain regions  cell types  and molecular pathways that may contribute to autism susceptibility. Overall design: Dissected heads of larval zebrafish and brains of adult zebrafish mutants in genes that increase risk for autism  which were generated using CRISPR/Cas9 mutagenesis.,,,,arid1b  adult  rep11,GSM8020226,,source name:single dissected adult brain  male|tissue:single dissected adult brain  male|genotype:arid1b heterozygous|geo loc name:missing|collection date:missing,arid1b  adult  rep11,Single end reads were aligned to GRCz11 release 104 using the Lawson Lab Zebrafish Transcriptome Annotation version 4.3.2 with STAR aligner 2.7.3a GCC 6.4.0 2.28. https://www.umassmed.edu/lawson lab/reagents/zebrafish transcriptome/ The raw counts files from STAR  in this record  were normalized using the rlog method in DESeq2 for subsequent published analysis. Assembly: GRCz11 Supplementary files format and content: Raw counts files from STAR; expected input for DESeq2.,single dissected adult brain  male,,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,tissue:single dissected adult brain  male|genotype:arid1b heterozygous,GSM8020226,GSM8020226: arid1b  adult  rep11; Danio rerio; RNA Seq,GSM8020226 r1,GSM8020226,1,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP484215,,loader:fastq load.py,arid1b-adult-set2-het_A7_S19_R1_001.fastq.gz,fastq,3609957150.0,35742150.0,GSM8020226 r1,0:101,A:997333826;C:818355353;G:803362992;T:990899286;N:5693,101,,,,997333826,818355353,803362992,990899286,5693,SRX23261718,SRS20163683,SRA1787174,UMass Chan Medical School,UMass Chan Medical School,,,,,,,,,,,,B,,usable mapping rate,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2024-01-16,Adult,Adult,Brain,Nervous System
29938,SRR27592963,SRX23261717,SRS20163682,SRP484215,PRJNA1065838,Diencephalic and Neuropeptidergic Dysfunction in Zebrafish with Autism Risk Mutations,GSE253405,Transcriptome Analysis,Hundreds of human mutations are linked to autism and related disorders  yet the functions of many of these mutated genes during vertebrate neural development are unclear. We generated 28 zebrafish mutants with presumptive protein truncating mutations or patient specific missense variants corresponding to autism risk alleles in 17 human genes. We observed baseline and stimulus driven behavioral changes at larval stages  as well as social behavior differences in lines tested as juveniles. Imaging whole brain activity revealed a near identical activity map for mutations in the unrelated genes kmt5b and hdlbpa  defined by increased activity mainly in the diencephalon. Truncating 7 of the 17 risk genes resulted in substantial brain size differences. Using RNA sequencing  we further defined molecular drivers of the observed phenotypes  identifying targetable disruptions in neuropeptide signaling  neuronal maturation  and cell proliferation. This multi modal screen has nominated brain regions  cell types  and molecular pathways that may contribute to autism susceptibility. Overall design: Dissected heads of larval zebrafish and brains of adult zebrafish mutants in genes that increase risk for autism  which were generated using CRISPR/Cas9 mutagenesis.,,,,arid1b  adult  rep10,GSM8020225,,source name:single dissected adult brain  male|tissue:single dissected adult brain  male|genotype:arid1b heterozygous|geo loc name:missing|collection date:missing,arid1b  adult  rep10,Single end reads were aligned to GRCz11 release 104 using the Lawson Lab Zebrafish Transcriptome Annotation version 4.3.2 with STAR aligner 2.7.3a GCC 6.4.0 2.28. https://www.umassmed.edu/lawson lab/reagents/zebrafish transcriptome/ The raw counts files from STAR  in this record  were normalized using the rlog method in DESeq2 for subsequent published analysis. Assembly: GRCz11 Supplementary files format and content: Raw counts files from STAR; expected input for DESeq2.,single dissected adult brain  male,,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,tissue:single dissected adult brain  male|genotype:arid1b heterozygous,GSM8020225,GSM8020225: arid1b  adult  rep10; Danio rerio; RNA Seq,GSM8020225 r1,GSM8020225,1,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP484215,,loader:fastq load.py,arid1b-adult-set2-het_A5_S17_R1_001.fastq.gz,fastq,3177521913.0,31460613.0,GSM8020225 r1,0:101,A:869322737;C:728803554;G:719191487;T:860199264;N:4871,101,,,,869322737,728803554,719191487,860199264,4871,SRX23261717,SRS20163682,SRA1787174,UMass Chan Medical School,UMass Chan Medical School,,,,,,,,,,,,B,,usable mapping rate,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2024-01-16,Adult,Adult,Brain,Nervous System
29939,SRR27592964,SRX23261716,SRS20163681,SRP484215,PRJNA1065838,Diencephalic and Neuropeptidergic Dysfunction in Zebrafish with Autism Risk Mutations,GSE253405,Transcriptome Analysis,Hundreds of human mutations are linked to autism and related disorders  yet the functions of many of these mutated genes during vertebrate neural development are unclear. We generated 28 zebrafish mutants with presumptive protein truncating mutations or patient specific missense variants corresponding to autism risk alleles in 17 human genes. We observed baseline and stimulus driven behavioral changes at larval stages  as well as social behavior differences in lines tested as juveniles. Imaging whole brain activity revealed a near identical activity map for mutations in the unrelated genes kmt5b and hdlbpa  defined by increased activity mainly in the diencephalon. Truncating 7 of the 17 risk genes resulted in substantial brain size differences. Using RNA sequencing  we further defined molecular drivers of the observed phenotypes  identifying targetable disruptions in neuropeptide signaling  neuronal maturation  and cell proliferation. This multi modal screen has nominated brain regions  cell types  and molecular pathways that may contribute to autism susceptibility. Overall design: Dissected heads of larval zebrafish and brains of adult zebrafish mutants in genes that increase risk for autism  which were generated using CRISPR/Cas9 mutagenesis.,,,,arid1b  adult  rep9,GSM8020224,,source name:single dissected adult brain  male|tissue:single dissected adult brain  male|genotype:arid1b heterozygous|geo loc name:missing|collection date:missing,arid1b  adult  rep9,Single end reads were aligned to GRCz11 release 104 using the Lawson Lab Zebrafish Transcriptome Annotation version 4.3.2 with STAR aligner 2.7.3a GCC 6.4.0 2.28. https://www.umassmed.edu/lawson lab/reagents/zebrafish transcriptome/ The raw counts files from STAR  in this record  were normalized using the rlog method in DESeq2 for subsequent published analysis. Assembly: GRCz11 Supplementary files format and content: Raw counts files from STAR; expected input for DESeq2.,single dissected adult brain  male,,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,tissue:single dissected adult brain  male|genotype:arid1b heterozygous,GSM8020224,GSM8020224: arid1b  adult  rep9; Danio rerio; RNA Seq,GSM8020224 r1,GSM8020224,1,RNA was extracted using the MicroElute Total RNA Kit Omega Bio Tek R6834 02  with a 15 min incubation with Dnase I. Libraries were constructed using an in house protocol based on SMART Seq2  followed by Nextera XT Library Preparation FC 131 1096.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina NovaSeq 6000,,SRP484215,,loader:fastq load.py,arid1b-adult-set2-het_A3_S15_R1_001.fastq.gz,fastq,3681640688.0,36451888.0,GSM8020224 r1,0:101,A:972550652;C:881056608;G:858084187;T:969943492;N:5749,101,,,,972550652,881056608,858084187,969943492,5749,SRX23261716,SRS20163681,SRA1787174,UMass Chan Medical School,UMass Chan Medical School,,,,,,,,,,,,B,,usable mapping rate,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2024-01-16,Adult,Adult,Brain,Nervous System