rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
101,DRR189403,DRX179868,DRS200418,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 3,SAMD00182246,,sample name:CSUS Tel 30 2w memory 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182246,DRX179868,CSUS Tel 30 2w memory 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182246,,,,2791119960.0,77531110.0,DRR189403,0:36,A:685050951;C:641519684;G:664655385;T:799677669;N:216271,36,,,,685050951,641519684,664655385,799677669,216271,DRX179868,DRS200418,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89653,,0.1773,,0.70725,,0.49873,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System
102,DRR189402,DRX179867,DRS200417,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 2,SAMD00182245,,sample name:CSUS Tel 30 2w memory 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182245,DRX179867,CSUS Tel 30 2w memory 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182245,,,,1505449224.0,41818034.0,DRR189402,0:36,A:369895057;C:346914787;G:358624651;T:429897489;N:117240,36,,,,369895057,346914787,358624651,429897489,117240,DRX179867,DRS200417,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89514,,0.17677,,0.7025,,0.49571,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System
103,DRR189401,DRX179866,DRS200416,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 1,SAMD00182244,,sample name:CSUS Tel 30 2w memory 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182244,DRX179866,CSUS Tel 30 2w memory 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182244,,,,2088507024.0,58014084.0,DRR189401,0:36,A:516255405;C:478036869;G:496415722;T:597637091;N:161937,36,,,,516255405,478036869,496415722,597637091,161937,DRX179866,DRS200416,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89533,,0.18553,,0.70981,,0.49554,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System
104,DRR189400,DRX179865,DRS200401,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 3,SAMD00182243,,sample name:CSUS Tel 30 1d memory 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182243,DRX179865,CSUS Tel 30 1d memory 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182243,,,,1340808120.0,37244670.0,DRR189400,0:36,A:330513975;C:306935584;G:320089399;T:383165274;N:103888,36,,,,330513975,306935584,320089399,383165274,103888,DRX179865,DRS200401,DRA008864,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89272,,0.19494,,0.70335,,0.49456,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
105,DRR189399,DRX179864,DRS200400,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 2,SAMD00182242,,sample name:CSUS Tel 30 1d memory 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182242,DRX179864,CSUS Tel 30 1d memory 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182242,,,,1279740096.0,35548336.0,DRR189399,0:36,A:314850837;C:294188093;G:304654881;T:365946483;N:99802,36,,,,314850837,294188093,304654881,365946483,99802,DRX179864,DRS200400,DRA008864,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89485,,0.18205,,0.70593,,0.49333,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
106,DRR189398,DRX179863,DRS200399,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 1,SAMD00182241,,sample name:CSUS Tel 30 1d memory 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182241,DRX179863,CSUS Tel 30 1d memory 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182241,,,,3012353100.0,83676475.0,DRR189398,0:36,A:740795057;C:693120664;G:717419185;T:860784745;N:233449,36,,,,740795057,693120664,717419185,860784745,233449,DRX179863,DRS200399,DRA008864,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89676,,0.1791,,0.70569,,0.49835,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
107,DRR189397,DRX179862,DRS200428,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post exposure to the conditioning tank 3,SAMD00182240,,sample name:Cont Tel 60 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182240,DRX179862,Cont Tel 60 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,500Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182240,,,,5590147750.0,111802955.0,DRR189397,0:50,A:1365131560;C:1269631295;G:1366791321;T:1588422020;N:171554,50,,,,1365131560,1269631295,1366791321,1588422020,171554,DRX179862,DRS200428,DRA008863,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89334,,0.16354,,0.7011,,0.49383,,50,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
108,DRR189396,DRX179861,DRS200427,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post exposure to the conditioning tank 2,SAMD00182239,,sample name:Cont Tel 60 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182239,DRX179861,Cont Tel 60 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,500Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182239,,,,6429019650.0,128580393.0,DRR189396,0:50,A:1570141412;C:1457539089;G:1572785681;T:1828358007;N:195461,50,,,,1570141412,1457539089,1572785681,1828358007,195461,DRX179861,DRS200427,DRA008863,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89595,,0.16204,,0.70743,,0.49805,,50,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
109,DRR189395,DRX179860,DRS200426,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post exposure to the conditioning tank 1,SAMD00182238,,sample name:Cont Tel 60 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182238,DRX179860,Cont Tel 60 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,500Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182238,,,,6644185850.0,132883717.0,DRR189395,0:50,A:1637322491;C:1498943556;G:1616212676;T:1891505597;N:201530,50,,,,1637322491,1498943556,1616212676,1891505597,201530,DRX179860,DRS200426,DRA008863,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89573,,0.16421,,0.7037,,0.49805,,50,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
110,DRR189394,DRX179859,DRS200407,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post light stimulation in the conditioning tank 3,SAMD00182237,,sample name:CS Tel 60 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182237,DRX179859,CS Tel 60 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182237,,,,1387078956.0,38529971.0,DRR189394,0:36,A:333990578;C:315361737;G:337599372;T:400057968;N:69301,36,,,,333990578,315361737,337599372,400057968,69301,DRX179859,DRS200407,DRA008862,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88133,,0.18418,,0.70747,,0.4971,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
111,DRR189393,DRX179858,DRS200406,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post light stimulation in the conditioning tank 2,SAMD00182236,,sample name:CS Tel 60 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182236,DRX179858,CS Tel 60 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182236,,,,537029424.0,14917484.0,DRR189393,0:36,A:127421487;C:122494467;G:132164579;T:154921150;N:27741,36,,,,127421487,122494467,132164579,154921150,27741,DRX179858,DRS200406,DRA008862,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88264,,0.17793,,0.7083,,0.49123,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
112,DRR189392,DRX179857,DRS200405,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post light stimulation in the conditioning tank 1,SAMD00182235,,sample name:CS Tel 60 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182235,DRX179857,CS Tel 60 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182235,,,,3163663656.0,87879546.0,DRR189392,0:36,A:766146692;C:714108562;G:771785405;T:911468887;N:154110,36,,,,766146692,714108562,771785405,911468887,154110,DRX179857,DRS200405,DRA008862,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88114,,0.1803,,0.7052,,0.4917,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
113,DRR189391,DRX179856,DRS200404,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post electrical shock delivery in the conditioning tank 3,SAMD00182234,,sample name:US Tel 60 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182234,DRX179856,US Tel 60 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182234,,,,1535884704.0,42663464.0,DRR189391,0:36,A:367444721;C:350978310;G:375524359;T:441859454;N:77860,36,,,,367444721,350978310,375524359,441859454,77860,DRX179856,DRS200404,DRA008861,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88103,,0.1788,,0.7082,,0.49462,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
114,DRR189390,DRX179855,DRS200403,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post electrical shock delivery in the conditioning tank 2,SAMD00182233,,sample name:US Tel 60 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182233,DRX179855,US Tel 60 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182233,,,,737309916.0,20480831.0,DRR189390,0:36,A:176982713;C:168284406;G:179343270;T:212662841;N:36686,36,,,,176982713,168284406,179343270,212662841,36686,DRX179855,DRS200403,DRA008861,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.87995,,0.18515,,0.70816,,0.49393,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
115,DRR189389,DRX179854,DRS200402,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post electrical shock delivery in the conditioning tank 1,SAMD00182232,,sample name:US Tel 60 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182232,DRX179854,US Tel 60 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182232,,,,595837404.0,16551039.0,DRR189389,0:36,A:143956004;C:134783754;G:145443580;T:171624939;N:29127,36,,,,143956004,134783754,145443580,171624939,29127,DRX179854,DRS200402,DRA008861,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.87682,,0.18328,,0.70309,,0.49081,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
116,DRR189388,DRX179853,DRS200452,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 3,SAMD00182231,,sample name:CSUS Tel 60 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182231,DRX179853,CSUS Tel 60 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182231,,,,608050044.0,16890279.0,DRR189388,0:36,A:145025778;C:137906052;G:149542672;T:175545338;N:30204,36,,,,145025778,137906052,149542672,175545338,30204,DRX179853,DRS200452,DRA008860,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.87983,,0.18602,,0.70445,,0.49195,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
117,DRR189387,DRX179852,DRS200451,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 2,SAMD00182230,,sample name:CSUS Tel 60 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182230,DRX179852,CSUS Tel 60 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182230,,,,777589452.0,21599707.0,DRR189387,0:36,A:185463269;C:177742981;G:190443063;T:223900096;N:40043,36,,,,185463269,177742981,190443063,223900096,40043,DRX179852,DRS200451,DRA008860,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.88331,,0.17918,,0.70516,,0.48956,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
118,DRR189386,DRX179851,DRS200450,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 1,SAMD00182229,,sample name:CSUS Tel 60 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182229,DRX179851,CSUS Tel 60 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182229,,,,2738622348.0,76072843.0,DRR189386,0:36,A:662989485;C:622023190;G:663013857;T:790459930;N:135886,36,,,,662989485,622023190,663013857,790459930,135886,DRX179851,DRS200450,DRA008860,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.87743,,0.19164,,0.70025,,0.49073,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
119,DRR189385,DRX179850,DRS200435,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of EMX3 / adult zebrafish 3,SAMD00182228,,sample name:Emx3 Adult Tel 3|genotype:Emx3 / |tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182228,DRX179850,Emx3 / Adult Tel 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182228,,,,1323636552.0,36767682.0,DRR189385,0:36,A:309582925;C:310206951;G:325673647;T:378136039;N:36990,36,,,,309582925,310206951,325673647,378136039,36990,DRX179850,DRS200435,DRA008859,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.8932,,0.19244,,0.71334,,0.50591,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
120,DRR189384,DRX179849,DRS200434,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of EMX3 / adult zebrafish 2,SAMD00182227,,sample name:Emx3 Adult Tel 2|genotype:Emx3 / |tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182227,DRX179849,Emx3 / Adult Tel 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182227,,,,2994105168.0,83169588.0,DRR189384,0:36,A:709730692;C:691531995;G:727245229;T:865514929;N:82323,36,,,,709730692,691531995,727245229,865514929,82323,DRX179849,DRS200434,DRA008859,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.90382,,0.1826,,0.70197,,0.49719,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
121,DRR189383,DRX179848,DRS200433,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of EMX3 / adult zebrafish 1,SAMD00182226,,sample name:Emx3 Adult Tel 1|genotype:Emx3 / |tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182226,DRX179848,Emx3 / Adult Tel 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182226,,,,1151464896.0,31985136.0,DRR189383,0:36,A:272427216;C:266708394;G:280781178;T:331515997;N:32111,36,,,,272427216,266708394,280781178,331515997,32111,DRX179848,DRS200433,DRA008859,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89942,,0.17402,,0.70364,,0.49438,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
122,DRR189382,DRX179847,DRS200421,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of wild type adult zebrafish 3,SAMD00182225,,sample name:WT Adult Tel 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182225,DRX179847,WT Adult Tel 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182225,,,,2837488824.0,78819134.0,DRR189382,0:36,A:678395895;C:655098137;G:687520693;T:816395518;N:78581,36,,,,678395895,655098137,687520693,816395518,78581,DRX179847,DRS200421,DRA008858,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89483,,0.18623,,0.70544,,0.49839,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
123,DRR189381,DRX179846,DRS200420,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of wild type adult zebrafish 2,SAMD00182224,,sample name:WT Adult Tel 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182224,DRX179846,WT Adult Tel 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182224,,,,1456800264.0,40466674.0,DRR189381,0:36,A:345320431;C:336838149;G:356264798;T:418336552;N:40334,36,,,,345320431,336838149,356264798,418336552,40334,DRX179846,DRS200420,DRA008858,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89496,,0.18691,,0.70802,,0.49721,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
124,DRR189380,DRX179845,DRS200419,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of wild type adult zebrafish 1,SAMD00182223,,sample name:WT Adult Tel 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182223,DRX179845,WT Adult Tel 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182223,,,,2533282452.0,70368957.0,DRR189380,0:36,A:602595083;C:585466052;G:616064286;T:729086926;N:70105,36,,,,602595083,585466052,616064286,729086926,70105,DRX179845,DRS200419,DRA008858,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89412,,0.19005,,0.70816,,0.49843,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
10237,ERR7131169,ERX6698608,ERS8070397,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 Nega,SAMEA10418613,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 Nega s,F8 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F22-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L006_R1_001.fastq.gz,fastq,658093749.0,12903799.0,E MTAB 11083:2874F22 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L006,0:51 1:0,A:173355262;C:152464129;G:147489024;T:184739885;N:45449,51,0,,,173355262,152464129,147489024,184739885,45449,ERX6698608,ERS8070397,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78328,,0.15147,,0.71289,,0.53671,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10238,ERR7131170,ERX6698608,ERS8070397,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 Nega,SAMEA10418613,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 Nega s,F8 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F22-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L007_R1_001.fastq.gz,fastq,661103769.0,12962819.0,E MTAB 11083:2874F22 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L007,0:51 1:0,A:174223249;C:153223284;G:148276805;T:185335092;N:45339,51,0,,,174223249,153223284,148276805,185335092,45339,ERX6698608,ERS8070397,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78539,,0.15054,,0.70897,,0.5322,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10239,ERR7131167,ERX6698607,ERS8070396,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 mCherry GFP,SAMEA10418612,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry GFP s,F8 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F24-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L006_R1_001.fastq.gz,fastq,693183636.0,13591836.0,E MTAB 11083:2874F24 1 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L006,0:51 1:0,A:183977687;C:159275153;G:153149826;T:196732022;N:48948,51,0,,,183977687,159275153,153149826,196732022,48948,ERX6698607,ERS8070396,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68942,,0.15593,,0.75828,,0.51858,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10240,ERR7131168,ERX6698607,ERS8070396,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 mCherry GFP,SAMEA10418612,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry GFP s,F8 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F24-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L007_R1_001.fastq.gz,fastq,696648678.0,13659778.0,E MTAB 11083:2874F24 2 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L007,0:51 1:0,A:184971832;C:160126524;G:154012023;T:197490098;N:48201,51,0,,,184971832,160126524,154012023,197490098,48201,ERX6698607,ERS8070396,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.6906,,0.15634,,0.75909,,0.51346,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10241,ERR7131165,ERX6698606,ERS8070395,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 mCherry,SAMEA10418611,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry s,F8 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F23-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L006_R1_001.fastq.gz,fastq,667760646.0,13093346.0,E MTAB 11083:2874F23 1 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L006,0:51 1:0,A:179948739;C:150541918;G:145038845;T:192184092;N:47052,51,0,,,179948739,150541918,145038845,192184092,47052,ERX6698606,ERS8070395,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71661,,0.19774,,0.74576,,0.52117,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10242,ERR7131166,ERX6698606,ERS8070395,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F8 mCherry,SAMEA10418611,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F8 mCherry s,F8 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F23-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L007_R1_001.fastq.gz,fastq,670970484.0,13156284.0,E MTAB 11083:2874F23 2 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L007,0:51 1:0,A:180906557;C:151329492;G:145816347;T:192872804;N:45284,51,0,,,180906557,151329492,145816347,192872804,45284,ERX6698606,ERS8070395,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71682,,0.19902,,0.74517,,0.52618,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10243,ERR7131163,ERX6698605,ERS8070394,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 Nega,SAMEA10418610,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 Nega s,F7 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F19-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L006_R1_001.fastq.gz,fastq,652205238.0,12788338.0,E MTAB 11083:2874F19 1 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L006,0:51 1:0,A:173730405;C:149044598;G:145436777;T:183946928;N:46530,51,0,,,173730405,149044598,145436777,183946928,46530,ERX6698605,ERS8070394,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80595,,0.17309,,0.71003,,0.53696,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10244,ERR7131164,ERX6698605,ERS8070394,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 Nega,SAMEA10418610,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 Nega s,F7 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F19-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L007_R1_001.fastq.gz,fastq,655593423.0,12854773.0,E MTAB 11083:2874F19 2 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L007,0:51 1:0,A:174715119;C:149844974;G:146250446;T:184737535;N:45349,51,0,,,174715119,149844974,146250446,184737535,45349,ERX6698605,ERS8070394,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80585,,0.17497,,0.71078,,0.53767,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10245,ERR7131161,ERX6698604,ERS8070393,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 mCherry GFP,SAMEA10418609,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry GFP s,F7 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F21-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L006_R1_001.fastq.gz,fastq,661067151.0,12962101.0,E MTAB 11083:2874F21 1 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L006,0:51 1:0,A:168281419;C:157701209;G:153487197;T:181550252;N:47074,51,0,,,168281419,157701209,153487197,181550252,47074,ERX6698604,ERS8070393,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.39074,,0.11592,,0.82615,,0.5252,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10246,ERR7131162,ERX6698604,ERS8070393,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 mCherry GFP,SAMEA10418609,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry GFP s,F7 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F21-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L007_R1_001.fastq.gz,fastq,665709018.0,13053118.0,E MTAB 11083:2874F21 2 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L007,0:51 1:0,A:169539998;C:158884603;G:154624803;T:182614264;N:45350,51,0,,,169539998,158884603,154624803,182614264,45350,ERX6698604,ERS8070393,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.38966,,0.1152,,0.8258,,0.53305,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10247,ERR7131159,ERX6698603,ERS8070392,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 mCherry,SAMEA10418608,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry s,F7 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F20-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L006_R1_001.fastq.gz,fastq,657813402.0,12898302.0,E MTAB 11083:2874F20 1 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L006,0:51 1:0,A:175873489;C:149476129;G:143987770;T:188429588;N:46426,51,0,,,175873489,149476129,143987770,188429588,46426,ERX6698603,ERS8070392,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70507,,0.2048,,0.75923,,0.52828,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10248,ERR7131160,ERX6698603,ERS8070392,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F7 mCherry,SAMEA10418608,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F7 mCherry s,F7 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F20-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L007_R1_001.fastq.gz,fastq,660303426.0,12947126.0,E MTAB 11083:2874F20 2 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L007,0:51 1:0,A:176607119;C:150065781;G:144613110;T:188972809;N:44607,51,0,,,176607119,150065781,144613110,188972809,44607,ERX6698603,ERS8070392,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70705,,0.20314,,0.75852,,0.52949,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10249,ERR7131157,ERX6698602,ERS8070391,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 Nega,SAMEA10418607,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 Nega s,F6 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F16-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L006_R1_001.fastq.gz,fastq,669627450.0,13129950.0,E MTAB 11083:2874F16 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L006,0:51 1:0,A:177483937;C:154097051;G:148063012;T:189935690;N:47760,51,0,,,177483937,154097051,148063012,189935690,47760,ERX6698602,ERS8070391,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.77183,,0.16001,,0.71467,,0.53352,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10250,ERR7131158,ERX6698602,ERS8070391,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 Nega,SAMEA10418607,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 Nega s,F6 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F16-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L007_R1_001.fastq.gz,fastq,671615073.0,13168923.0,E MTAB 11083:2874F16 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L007,0:51 1:0,A:178109157;C:154619024;G:148595207;T:190246469;N:45216,51,0,,,178109157,154619024,148595207,190246469,45216,ERX6698602,ERS8070391,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.77284,,0.15963,,0.71569,,0.53038,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10251,ERR7131155,ERX6698601,ERS8070390,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 mCherry GFP,SAMEA10418606,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry GFP s,F6 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F18-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L006_R1_001.fastq.gz,fastq,667663542.0,13091442.0,E MTAB 11083:2874F18 1 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L006,0:51 1:0,A:177646483;C:152747528;G:146388226;T:190833951;N:47354,51,0,,,177646483,152747528,146388226,190833951,47354,ERX6698601,ERS8070390,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67964,,0.18123,,0.77193,,0.53313,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10252,ERR7131156,ERX6698601,ERS8070390,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 mCherry GFP,SAMEA10418606,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry GFP s,F6 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F18-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L007_R1_001.fastq.gz,fastq,670170141.0,13140591.0,E MTAB 11083:2874F18 2 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L007,0:51 1:0,A:178425395;C:153363855;G:147033779;T:191300976;N:46136,51,0,,,178425395,153363855,147033779,191300976,46136,ERX6698601,ERS8070390,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67922,,0.18101,,0.77141,,0.53601,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10253,ERR7131153,ERX6698600,ERS8070389,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 mCherry,SAMEA10418605,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry s,F6 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F17-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L006_R1_001.fastq.gz,fastq,677617008.0,13286608.0,E MTAB 11083:2874F17 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L006,0:51 1:0,A:181785899;C:152797687;G:147070676;T:195914808;N:47938,51,0,,,181785899,152797687,147070676,195914808,47938,ERX6698600,ERS8070389,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68298,,0.1953,,0.76292,,0.5395,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10254,ERR7131154,ERX6698600,ERS8070389,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F6 mCherry,SAMEA10418605,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F6 mCherry s,F6 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F17-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L007_R1_001.fastq.gz,fastq,679922973.0,13331823.0,E MTAB 11083:2874F17 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L007,0:51 1:0,A:182567819;C:153380607;G:147589890;T:196337722;N:46935,51,0,,,182567819,153380607,147589890,196337722,46935,ERX6698600,ERS8070389,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68391,,0.19726,,0.76299,,0.54099,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10255,ERR7131151,ERX6698599,ERS8070388,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 Nega,SAMEA10418604,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 Nega s,F5 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F13-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L006_R1_001.fastq.gz,fastq,620316315.0,12163065.0,E MTAB 11083:2874F13 1 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L006,0:51 1:0,A:165967355;C:141072216;G:136931219;T:176301963;N:43562,51,0,,,165967355,141072216,136931219,176301963,43562,ERX6698599,ERS8070388,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78924,,0.17457,,0.71934,,0.53563,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10256,ERR7131152,ERX6698599,ERS8070388,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 Nega,SAMEA10418604,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 Nega s,F5 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F13-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L007_R1_001.fastq.gz,fastq,623905338.0,12233438.0,E MTAB 11083:2874F13 2 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L007,0:51 1:0,A:166996253;C:141937637;G:137825206;T:177103504;N:42738,51,0,,,166996253,141937637,137825206,177103504,42738,ERX6698599,ERS8070388,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78975,,0.17387,,0.72153,,0.54149,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10257,ERR7131149,ERX6698598,ERS8070387,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 mCherry GFP,SAMEA10418603,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry GFP s,F5 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F15-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L006_R1_001.fastq.gz,fastq,670154841.0,13140291.0,E MTAB 11083:2874F15 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L006,0:51 1:0,A:182457480;C:149175456;G:144548173;T:193927470;N:46262,51,0,,,182457480,149175456,144548173,193927470,46262,ERX6698598,ERS8070387,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71875,,0.18671,,0.76047,,0.52398,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10258,ERR7131150,ERX6698598,ERS8070387,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 mCherry GFP,SAMEA10418603,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry GFP s,F5 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F15-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L007_R1_001.fastq.gz,fastq,674202048.0,13219648.0,E MTAB 11083:2874F15 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L007,0:51 1:0,A:183639511;C:150168226;G:145502071;T:194847755;N:44485,51,0,,,183639511,150168226,145502071,194847755,44485,ERX6698598,ERS8070387,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.72111,,0.18813,,0.76378,,0.5258,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10259,ERR7131147,ERX6698597,ERS8070386,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 mCherry,SAMEA10418602,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry s,F5 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F14-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L006_R1_001.fastq.gz,fastq,615458259.0,12067809.0,E MTAB 11083:2874F14 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L006,0:51 1:0,A:165108582;C:139178883;G:134070672;T:177059757;N:40365,51,0,,,165108582,139178883,134070672,177059757,40365,ERX6698597,ERS8070386,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69017,,0.1977,,0.77193,,0.53352,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10260,ERR7131148,ERX6698597,ERS8070386,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F5 mCherry,SAMEA10418602,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F5 mCherry s,F5 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F14-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L007_R1_001.fastq.gz,fastq,619061613.0,12138463.0,E MTAB 11083:2874F14 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L007,0:51 1:0,A:166167516;C:140058774;G:134972850;T:177822733;N:39740,51,0,,,166167516,140058774,134972850,177822733,39740,ERX6698597,ERS8070386,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69118,,0.20019,,0.7707,,0.52174,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10261,ERR7131145,ERX6698596,ERS8070385,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 Nega,SAMEA10418601,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 Nega s,F4 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F10-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L006_R1_001.fastq.gz,fastq,652536687.0,12794837.0,E MTAB 11083:2874F10 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L006,0:51 1:0,A:178033231;C:144661707;G:139876661;T:189921085;N:44003,51,0,,,178033231,144661707,139876661,189921085,44003,ERX6698596,ERS8070385,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.76201,,0.2508,,0.71299,,0.53379,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10262,ERR7131146,ERX6698596,ERS8070385,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 Nega,SAMEA10418601,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 Nega s,F4 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F10-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L007_R1_001.fastq.gz,fastq,655278957.0,12848607.0,E MTAB 11083:2874F10 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L007,0:51 1:0,A:178938002;C:145332613;G:140527756;T:190437189;N:43397,51,0,,,178938002,145332613,140527756,190437189,43397,ERX6698596,ERS8070385,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.76188,,0.25356,,0.71344,,0.53081,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10263,ERR7131143,ERX6698595,ERS8070384,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 mCherry GFP,SAMEA10418600,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry GFP s,F4 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F12-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L006_R1_001.fastq.gz,fastq,297921855.0,5841605.0,E MTAB 11083:2874F12 1 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L006,0:51 1:0,A:82400724;C:65517496;G:64603058;T:85384480;N:16097,51,0,,,82400724,65517496,64603058,85384480,16097,ERX6698595,ERS8070384,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.72496,,0.20794,,0.81223,,0.52529,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10264,ERR7131144,ERX6698595,ERS8070384,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 mCherry GFP,SAMEA10418600,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry GFP s,F4 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F12-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L007_R1_001.fastq.gz,fastq,309412971.0,6066921.0,E MTAB 11083:2874F12 2 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L007,0:51 1:0,A:85531271;C:68164437;G:67129209;T:88573210;N:14844,51,0,,,85531271,68164437,67129209,88573210,14844,ERX6698595,ERS8070384,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.7245,,0.20724,,0.80468,,0.52791,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10265,ERR7131141,ERX6698594,ERS8070383,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 mCherry,SAMEA10418599,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry s,F4 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F11-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L006_R1_001.fastq.gz,fastq,672319791.0,13182741.0,E MTAB 11083:2874F11 1 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L006,0:51 1:0,A:181508034;C:150928549;G:146124085;T:193711358;N:47765,51,0,,,181508034,150928549,146124085,193711358,47765,ERX6698594,ERS8070383,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71726,,0.19842,,0.74986,,0.53363,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10266,ERR7131142,ERX6698594,ERS8070383,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F4 mCherry,SAMEA10418599,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F4 mCherry s,F4 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F11-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L007_R1_001.fastq.gz,fastq,675486075.0,13244825.0,E MTAB 11083:2874F11 2 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L007,0:51 1:0,A:182462261;C:151688554;G:146902713;T:194385945;N:46602,51,0,,,182462261,151688554,146902713,194385945,46602,ERX6698594,ERS8070383,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.71745,,0.19727,,0.74805,,0.53092,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10267,ERR7131139,ERX6698593,ERS8070382,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 Nega,SAMEA10418598,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 Nega s,F3 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F7-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L006_R1_001.fastq.gz,fastq,630093627.0,12354777.0,E MTAB 11083:2874F7 1 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L006,0:51 1:0,A:165132739;C:146179836;G:142796880;T:175940613;N:43559,51,0,,,165132739,146179836,142796880,175940613,43559,ERX6698593,ERS8070382,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81672,,0.15219,,0.71277,,0.52461,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10268,ERR7131140,ERX6698593,ERS8070382,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 Nega,SAMEA10418598,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 Nega s,F3 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F7-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L007_R1_001.fastq.gz,fastq,632305242.0,12398142.0,E MTAB 11083:2874F7 2 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L007,0:51 1:0,A:165832174;C:146751895;G:143384539;T:176293313;N:43321,51,0,,,165832174,146751895,143384539,176293313,43321,ERX6698593,ERS8070382,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81791,,0.15444,,0.7151,,0.52585,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10269,ERR7131137,ERX6698592,ERS8070381,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 mCherry GFP,SAMEA10418597,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry GFP s,F3 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F9-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L006_R1_001.fastq.gz,fastq,660379620.0,12948620.0,E MTAB 11083:2874F9 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L006,0:51 1:0,A:178482695;C:147417787;G:143797476;T:190635173;N:46489,51,0,,,178482695,147417787,143797476,190635173,46489,ERX6698592,ERS8070381,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.65635,,0.17778,,0.76899,,0.52957,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10270,ERR7131138,ERX6698592,ERS8070381,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 mCherry GFP,SAMEA10418597,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry GFP s,F3 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F9-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L007_R1_001.fastq.gz,fastq,662411307.0,12988457.0,E MTAB 11083:2874F9 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L007,0:51 1:0,A:179158323;C:147942300;G:144319925;T:190945370;N:45389,51,0,,,179158323,147942300,144319925,190945370,45389,ERX6698592,ERS8070381,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.65813,,0.17896,,0.77076,,0.52835,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10271,ERR7131135,ERX6698591,ERS8070380,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 mCherry,SAMEA10418596,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry s,F3 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F8-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L006_R1_001.fastq.gz,fastq,698608353.0,13698203.0,E MTAB 11083:2874F8 1 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L006,0:51 1:0,A:188718612;C:156814081;G:151125083;T:201901570;N:49007,51,0,,,188718612,156814081,151125083,201901570,49007,ERX6698591,ERS8070380,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70185,,0.21547,,0.75588,,0.53853,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10272,ERR7131136,ERX6698591,ERS8070380,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F3 mCherry,SAMEA10418596,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F3 mCherry s,F3 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F8-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L007_R1_001.fastq.gz,fastq,699869175.0,13722925.0,E MTAB 11083:2874F8 2 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L007,0:51 1:0,A:189192315;C:157176897;G:151498983;T:201952867;N:48113,51,0,,,189192315,157176897,151498983,201952867,48113,ERX6698591,ERS8070380,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.70221,,0.21684,,0.75621,,0.53787,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10273,ERR7131133,ERX6698590,ERS8070379,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 Nega,SAMEA10418595,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 Nega s,F2 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F4-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L006_R1_001.fastq.gz,fastq,573812985.0,11251235.0,E MTAB 11083:2874F4 1 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L006,0:51 1:0,A:150716404;C:132203167;G:129732968;T:161120057;N:40389,51,0,,,150716404,132203167,129732968,161120057,40389,ERX6698590,ERS8070379,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81899,,0.17302,,0.72281,,0.54315,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10274,ERR7131134,ERX6698590,ERS8070379,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 Nega,SAMEA10418595,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 Nega s,F2 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F4-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L007_R1_001.fastq.gz,fastq,576184587.0,11297737.0,E MTAB 11083:2874F4 2 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L007,0:51 1:0,A:151387408;C:132788210;G:130392043;T:161577529;N:39397,51,0,,,151387408,132788210,130392043,161577529,39397,ERX6698590,ERS8070379,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.81999,,0.17082,,0.72196,,0.54505,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10275,ERR7131131,ERX6698589,ERS8070378,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 mCherry GFP,SAMEA10418594,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry GFP s,F2 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F6-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L006_R1_001.fastq.gz,fastq,654811032.0,12839432.0,E MTAB 11083:2874F6 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L006,0:51 1:0,A:177403486;C:146202733;G:142207545;T:188951373;N:45895,51,0,,,177403486,146202733,142207545,188951373,45895,ERX6698589,ERS8070378,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.67854,,0.21407,,0.76104,,0.52713,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10276,ERR7131132,ERX6698589,ERS8070378,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 mCherry GFP,SAMEA10418594,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry GFP s,F2 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F6-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L007_R1_001.fastq.gz,fastq,656911926.0,12880626.0,E MTAB 11083:2874F6 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L007,0:51 1:0,A:178119212;C:146730132;G:142725348;T:189293212;N:44022,51,0,,,178119212,146730132,142725348,189293212,44022,ERX6698589,ERS8070378,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.68162,,0.21431,,0.761,,0.5071,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10277,ERR7131129,ERX6698588,ERS8070377,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 mCherry,SAMEA10418593,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry s,F2 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F5-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L006_R1_001.fastq.gz,fastq,607851558.0,11918658.0,E MTAB 11083:2874F5 1 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L006,0:51 1:0,A:164814138;C:136071648;G:132945950;T:173976881;N:42941,51,0,,,164814138,136071648,132945950,173976881,42941,ERX6698588,ERS8070377,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78967,,0.18905,,0.73241,,0.52347,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10278,ERR7131130,ERX6698588,ERS8070377,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F2 mCherry,SAMEA10418593,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F2 mCherry s,F2 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F5-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L007_R1_001.fastq.gz,fastq,610931805.0,11979055.0,E MTAB 11083:2874F5 2 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L007,0:51 1:0,A:165714775;C:136822925;G:133684294;T:174667814;N:41997,51,0,,,165714775,136822925,133684294,174667814,41997,ERX6698588,ERS8070377,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.78869,,0.18885,,0.73156,,0.51691,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10279,ERR7131127,ERX6698587,ERS8070376,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 Nega,SAMEA10418592,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 Nega s,F1 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F1-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L006_R1_001.fastq.gz,fastq,619561872.0,12148272.0,E MTAB 11083:2874F1 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L006,0:51 1:0,A:164385545;C:141746313;G:138163219;T:175223323;N:43472,51,0,,,164385545,141746313,138163219,175223323,43472,ERX6698587,ERS8070376,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80258,,0.17703,,0.72614,,0.53483,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10280,ERR7131128,ERX6698587,ERS8070376,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 Nega,SAMEA10418592,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 Nega s,F1 Nega s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry /GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F1-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L007_R1_001.fastq.gz,fastq,621481971.0,12185921.0,E MTAB 11083:2874F1 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L007,0:51 1:0,A:165030774;C:142231469;G:138651078;T:175527080;N:41570,51,0,,,165030774,142231469,138651078,175527080,41570,ERX6698587,ERS8070376,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.80248,,0.17699,,0.726,,0.53711,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10281,ERR7131125,ERX6698586,ERS8070375,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 mCherry GFP,SAMEA10418591,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry GFP s,F1 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F3-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L006_R1_001.fastq.gz,fastq,651189369.0,12768419.0,E MTAB 11083:2874F3 1 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L006,0:51 1:0,A:176398415;C:145659111;G:142137610;T:186948280;N:45953,51,0,,,176398415,145659111,142137610,186948280,45953,ERX6698586,ERS8070375,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69561,,0.18489,,0.75345,,0.52316,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10282,ERR7131126,ERX6698586,ERS8070375,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 mCherry GFP,SAMEA10418591,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry GFP s,F1 mCherry GFP s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP+,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F3-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L007_R1_001.fastq.gz,fastq,653398944.0,12811744.0,E MTAB 11083:2874F3 2 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L007,0:51 1:0,A:177139734;C:146171766;G:142707146;T:187335475;N:44823,51,0,,,177139734,146171766,142707146,187335475,44823,ERX6698586,ERS8070375,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.69496,,0.18414,,0.75375,,0.52317,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10283,ERR7131123,ERX6698585,ERS8070374,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 mCherry,SAMEA10418590,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry s,F1 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F2-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L006_R1_001.fastq.gz,fastq,613574319.0,12030869.0,E MTAB 11083:2874F2 1 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L006,0:51 1:0,A:164891629;C:138401759;G:135818073;T:174419828;N:43030,51,0,,,164891629,138401759,135818073,174419828,43030,ERX6698585,ERS8070374,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.79499,,0.21674,,0.73359,,0.52207,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10284,ERR7131124,ERX6698585,ERS8070374,ERP132560,PRJEB48218,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E-MTAB-11083,Transcriptome Analysis,To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,,Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,F1 mCherry,SAMEA10418590,Friedrich Miescher Institute for Biomedical Research,ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK,,,,,,,,,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,E MTAB 11083:F1 mCherry s,F1 mCherry s,Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers.,Experimental Factor: fraction:mCherry+/GFP ,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,ILLUMINA,Illumina HiSeq 2500,,ERP132560,Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb,ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07,2874F2-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L007_R1_001.fastq.gz,fastq,615666798.0,12071898.0,E MTAB 11083:2874F2 2 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L007,0:51 1:0,A:165536502;C:138952758;G:136375193;T:174760659;N:41686,51,0,,,165536502,138952758,136375193,174760659,41686,ERX6698585,ERS8070374,ERA6757553,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive,1,0.7941,,0.21645,,0.73494,,0.50761,,51,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,nextera,sc,single_cell_plate,smartseq,,Switzerland,2021-12-01,Adult,Adult,Brain,Nervous System
10407,ERR9077202,ERX8620336,ERS10857224,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT5S8,SAMEA13254181,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT5S8 s,Sv2aWT5S8 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT5S8L001-1.fastq.gz,fastq,652046291.0,8633105.0,E MTAB 11505:sv2aWT5S8L001,0:75.53 1:0,A:159570243;C:156420495;G:154853315;T:181073030;N:129208,75,0,,,159570243,156420495,154853315,181073030,129208,ERX8620336,ERS10857224,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95245,,0.1148,,0.65756,,0.47011,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10408,ERR9077203,ERX8620336,ERS10857224,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT5S8,SAMEA13254181,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT5S8 s,Sv2aWT5S8 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT5S8L002-1.fastq.gz,fastq,653840525.0,8656886.0,E MTAB 11505:sv2aWT5S8L002,0:75.53 1:0,A:159902458;C:156826842;G:155509070;T:181475104;N:127051,75,0,,,159902458,156826842,155509070,181475104,127051,ERX8620336,ERS10857224,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95226,,0.1156,,0.65792,,0.4667,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10409,ERR9077204,ERX8620336,ERS10857224,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT5S8,SAMEA13254181,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT5S8 s,Sv2aWT5S8 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT5S8L003-1.fastq.gz,fastq,659584196.0,8732885.0,E MTAB 11505:sv2aWT5S8L003,0:75.53 1:0,A:161257146;C:158299734;G:156793443;T:183128972;N:104901,75,0,,,161257146,158299734,156793443,183128972,104901,ERX8620336,ERS10857224,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95298,,0.11485,,0.65798,,0.46853,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10410,ERR9077205,ERX8620336,ERS10857224,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT5S8,SAMEA13254181,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT5S8 s,Sv2aWT5S8 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT5S8L004-1.fastq.gz,fastq,666627746.0,8826123.0,E MTAB 11505:sv2aWT5S8L004,0:75.53 1:0,A:162975131;C:159937258;G:158506242;T:185106149;N:102966,75,0,,,162975131,159937258,158506242,185106149,102966,ERX8620336,ERS10857224,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.9531,,0.11425,,0.65736,,0.47309,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10411,ERR9077198,ERX8620335,ERS10857223,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT4S7,SAMEA13254180,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT4S7 s,Sv2aWT4S7 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT4S7L001-1.fastq.gz,fastq,683751258.0,9051795.0,E MTAB 11505:sv2aWT4S7L001,0:75.54 1:0,A:162889923;C:167127644;G:164779384;T:188822907;N:131400,75,0,,,162889923,167127644,164779384,188822907,131400,ERX8620335,ERS10857223,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95739,,0.0959,,0.6606,,0.46826,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10412,ERR9077199,ERX8620335,ERS10857223,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT4S7,SAMEA13254180,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT4S7 s,Sv2aWT4S7 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT4S7L002-1.fastq.gz,fastq,684187711.0,9057591.0,E MTAB 11505:sv2aWT4S7L002,0:75.54 1:0,A:162923879;C:167246213;G:165071145;T:188818774;N:127700,75,0,,,162923879,167246213,165071145,188818774,127700,ERX8620335,ERS10857223,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95724,,0.0959,,0.66036,,0.47026,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10413,ERR9077200,ERX8620335,ERS10857223,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT4S7,SAMEA13254180,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT4S7 s,Sv2aWT4S7 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT4S7L003-1.fastq.gz,fastq,692320213.0,9165132.0,E MTAB 11505:sv2aWT4S7L003,0:75.54 1:0,A:164772792;C:169329920;G:166952052;T:191164422;N:101027,75,0,,,164772792,169329920,166952052,191164422,101027,ERX8620335,ERS10857223,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95786,,0.09701,,0.66131,,0.47564,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10414,ERR9077201,ERX8620335,ERS10857223,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT4S7,SAMEA13254180,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT4S7 s,Sv2aWT4S7 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT4S7L004-1.fastq.gz,fastq,697047881.0,9227702.0,E MTAB 11505:sv2aWT4S7L004,0:75.54 1:0,A:165881728;C:170388393;G:168105900;T:192572068;N:99792,75,0,,,165881728,170388393,168105900,192572068,99792,ERX8620335,ERS10857223,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.9584,,0.09565,,0.66115,,0.46967,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10415,ERR9077194,ERX8620334,ERS10857222,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT3S2,SAMEA13254179,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT3S2 s,Sv2aWT3S2 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT3S2L001-1.fastq.gz,fastq,723508205.0,9579193.0,E MTAB 11505:sv2aWT3S2L001,0:75.53 1:0,A:177832489;C:174193135;G:169978598;T:201360184;N:143799,75,0,,,177832489,174193135,169978598,201360184,143799,ERX8620334,ERS10857222,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95218,,0.11732,,0.65916,,0.47955,,71,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10416,ERR9077195,ERX8620334,ERS10857222,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT3S2,SAMEA13254179,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT3S2 s,Sv2aWT3S2 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT3S2L002-1.fastq.gz,fastq,726190024.0,9614674.0,E MTAB 11505:sv2aWT3S2L002,0:75.53 1:0,A:178408224;C:174776263;G:170820745;T:202047224;N:137568,75,0,,,178408224,174776263,170820745,202047224,137568,ERX8620334,ERS10857222,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95093,,0.11567,,0.65865,,0.46877,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10417,ERR9077196,ERX8620334,ERS10857222,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT3S2,SAMEA13254179,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT3S2 s,Sv2aWT3S2 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT3S2L003-1.fastq.gz,fastq,735499955.0,9737845.0,E MTAB 11505:sv2aWT3S2L003,0:75.53 1:0,A:180659962;C:177141362;G:172905323;T:204678308;N:115000,75,0,,,180659962,177141362,172905323,204678308,115000,ERX8620334,ERS10857222,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95233,,0.11614,,0.65898,,0.47114,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10418,ERR9077197,ERX8620334,ERS10857222,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT3S2,SAMEA13254179,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT3S2 s,Sv2aWT3S2 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT3S2L004-1.fastq.gz,fastq,745285512.0,9867337.0,E MTAB 11505:sv2aWT3S2L004,0:75.53 1:0,A:183067487;C:179408382;G:175250814;T:207445912;N:112917,75,0,,,183067487,179408382,175250814,207445912,112917,ERX8620334,ERS10857222,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95245,,0.11655,,0.65857,,0.47087,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10419,ERR9077190,ERX8620333,ERS10857221,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT1S1,SAMEA13254178,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT1S1 s,Sv2aWT1S1 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT1S1L001-1.fastq.gz,fastq,647414748.0,8571883.0,E MTAB 11505:sv2aWT1S1L001,0:75.53 1:0,A:154593859;C:160498057;G:153809612;T:178389112;N:124108,75,0,,,154593859,160498057,153809612,178389112,124108,ERX8620333,ERS10857221,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95955,,0.08686,,0.65955,,0.47406,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10420,ERR9077191,ERX8620333,ERS10857221,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT1S1,SAMEA13254178,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT1S1 s,Sv2aWT1S1 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT1S1L002-1.fastq.gz,fastq,646657758.0,8561861.0,E MTAB 11505:sv2aWT1S1L002,0:75.53 1:0,A:154348044;C:160294625;G:153804048;T:178092212;N:118829,75,0,,,154348044,160294625,153804048,178092212,118829,ERX8620333,ERS10857221,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95942,,0.08632,,0.65991,,0.47539,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10421,ERR9077192,ERX8620333,ERS10857221,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT1S1,SAMEA13254178,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT1S1 s,Sv2aWT1S1 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT1S1L003-1.fastq.gz,fastq,654781451.0,8669333.0,E MTAB 11505:sv2aWT1S1L003,0:75.53 1:0,A:156252744;C:162396721;G:155681450;T:180352911;N:97625,75,0,,,156252744,162396721,155681450,180352911,97625,ERX8620333,ERS10857221,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95972,,0.08498,,0.66137,,0.47457,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10422,ERR9077193,ERX8620333,ERS10857221,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT1S1,SAMEA13254178,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT1S1 s,Sv2aWT1S1 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT1S1L004-1.fastq.gz,fastq,659716937.0,8734618.0,E MTAB 11505:sv2aWT1S1L004,0:75.53 1:0,A:157367211;C:163585842;G:156911139;T:181757809;N:94936,75,0,,,157367211,163585842,156911139,181757809,94936,ERX8620333,ERS10857221,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95985,,0.08585,,0.65857,,0.47349,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10423,ERR9077186,ERX8620332,ERS10857220,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO6S12,SAMEA13254177,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO6S12 s,Sv2aHO6S12 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO6S12L001-1.fastq.gz,fastq,675018261.0,8937958.0,E MTAB 11505:sv2aHO6S12L001,0:75.52 1:0,A:166564263;C:160539328;G:160003980;T:187773509;N:137181,75,0,,,166564263,160539328,160003980,187773509,137181,ERX8620332,ERS10857220,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94964,,0.13009,,0.65413,,0.47079,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10424,ERR9077187,ERX8620332,ERS10857220,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO6S12,SAMEA13254177,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO6S12 s,Sv2aHO6S12 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO6S12L002-1.fastq.gz,fastq,672995667.0,8911161.0,E MTAB 11505:sv2aHO6S12L002,0:75.52 1:0,A:165990319;C:160039398;G:159722501;T:187108781;N:134668,75,0,,,165990319,160039398,159722501,187108781,134668,ERX8620332,ERS10857220,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94837,,0.13029,,0.65744,,0.47959,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10425,ERR9077188,ERX8620332,ERS10857220,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO6S12,SAMEA13254177,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO6S12 s,Sv2aHO6S12 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO6S12L003-1.fastq.gz,fastq,683276767.0,9047147.0,E MTAB 11505:sv2aHO6S12L003,0:75.52 1:0,A:168501690;C:162583852;G:162063190;T:190018001;N:110034,75,0,,,168501690,162583852,162063190,190018001,110034,ERX8620332,ERS10857220,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94983,,0.13248,,0.6565,,0.47977,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10426,ERR9077189,ERX8620332,ERS10857220,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO6S12,SAMEA13254177,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO6S12 s,Sv2aHO6S12 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO6S12L004-1.fastq.gz,fastq,686469910.0,9089431.0,E MTAB 11505:sv2aHO6S12L004,0:75.52 1:0,A:169287189;C:163276361;G:162844724;T:190954218;N:107418,75,0,,,169287189,163276361,162844724,190954218,107418,ERX8620332,ERS10857220,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95061,,0.13029,,0.65389,,0.48044,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10427,ERR9077182,ERX8620331,ERS10857219,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO4S10,SAMEA13254176,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO4S10 s,Sv2aHO4S10 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO4S10L001-1.fastq.gz,fastq,713858966.0,9452474.0,E MTAB 11505:sv2aHO4S10L001,0:75.52 1:0,A:177913695;C:169425317;G:167540012;T:198835532;N:144410,75,0,,,177913695,169425317,167540012,198835532,144410,ERX8620331,ERS10857219,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94848,,0.12981,,0.6636,,0.47772,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10428,ERR9077183,ERX8620331,ERS10857219,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO4S10,SAMEA13254176,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO4S10 s,Sv2aHO4S10 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO4S10L002-1.fastq.gz,fastq,717619593.0,9502301.0,E MTAB 11505:sv2aHO4S10L002,0:75.52 1:0,A:178830542;C:170284720;G:168594514;T:199773382;N:136435,75,0,,,178830542,170284720,168594514,199773382,136435,ERX8620331,ERS10857219,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94812,,0.12868,,0.6645,,0.47281,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10429,ERR9077184,ERX8620331,ERS10857219,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO4S10,SAMEA13254176,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO4S10 s,Sv2aHO4S10 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO4S10L003-1.fastq.gz,fastq,727263626.0,9629858.0,E MTAB 11505:sv2aHO4S10L003,0:75.52 1:0,A:181201278;C:172619520;G:170780953;T:202550397;N:111478,75,0,,,181201278,172619520,170780953,202550397,111478,ERX8620331,ERS10857219,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94768,,0.12827,,0.66596,,0.47332,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10430,ERR9077185,ERX8620331,ERS10857219,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO4S10,SAMEA13254176,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO4S10 s,Sv2aHO4S10 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO4S10L004-1.fastq.gz,fastq,736595278.0,9753337.0,E MTAB 11505:sv2aHO4S10L004,0:75.52 1:0,A:183510872;C:174806534;G:173027794;T:205140640;N:109438,75,0,,,183510872,174806534,173027794,205140640,109438,ERX8620331,ERS10857219,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94925,,0.12904,,0.66417,,0.47297,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10431,ERR9077178,ERX8620330,ERS10857218,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO3S6,SAMEA13254175,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO3S6 s,Sv2aHO3S6 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO3S6L001-1.fastq.gz,fastq,710756305.0,9410862.0,E MTAB 11505:sv2aHO3S6L001,0:75.53 1:0,A:175585613;C:170261120;G:166658917;T:198106388;N:144267,75,0,,,175585613,170261120,166658917,198106388,144267,ERX8620330,ERS10857218,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94827,,0.14002,,0.66785,,0.474,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10432,ERR9077179,ERX8620330,ERS10857218,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO3S6,SAMEA13254175,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO3S6 s,Sv2aHO3S6 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO3S6L002-1.fastq.gz,fastq,715330324.0,9471415.0,E MTAB 11505:sv2aHO3S6L002,0:75.53 1:0,A:176679775;C:171311031;G:167903237;T:199299483;N:136798,75,0,,,176679775,171311031,167903237,199299483,136798,ERX8620330,ERS10857218,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.9469,,0.13915,,0.67004,,0.47654,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10433,ERR9077180,ERX8620330,ERS10857218,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO3S6,SAMEA13254175,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO3S6 s,Sv2aHO3S6 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO3S6L003-1.fastq.gz,fastq,722810562.0,9570364.0,E MTAB 11505:sv2aHO3S6L003,0:75.53 1:0,A:178442845;C:173192786;G:169595085;T:201465522;N:114324,75,0,,,178442845,173192786,169595085,201465522,114324,ERX8620330,ERS10857218,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94918,,0.13979,,0.66902,,0.47572,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System
10434,ERR9077181,ERX8620330,ERS10857218,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO3S6,SAMEA13254175,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO3S6 s,Sv2aHO3S6 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO3S6L004-1.fastq.gz,fastq,732990507.0,9705054.0,E MTAB 11505:sv2aHO3S6L004,0:75.53 1:0,A:180951130;C:175567778;G:172037542;T:204323488;N:110569,75,0,,,180951130,175567778,172037542,204323488,110569,ERX8620330,ERS10857218,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94812,,0.13893,,0.67085,,0.47502,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System