rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 62742,SRR13360748,SRX9785053,SRS7972286,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,macrogard,macrogard2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:macrogardPM replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,PM2,PM2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,pm2_S8_R1_001.fastq.gz,fastq,3517898944.0,46288144.0,pm2 S8 R1 001.fastq.gz,0:76 1:0,A:914616261;C:828363626;G:805674434;T:969167482;N:77141,76,0,,,914616261,828363626,805674434,969167482,77141,SRX9785053,SRS7972286,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.94008,,0.0747,,0.71394,,0.51572,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62743,SRR13360749,SRX9785052,SRS7972285,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,macrogard,macrogard1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:macrogardPM replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,PM1,PM1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,pm1_S7_R1_001.fastq.gz,fastq,1974822380.0,25984505.0,pm1 S7 R1 001.fastq.gz,0:76 1:0,A:561606319;C:416363486;G:408486827;T:588324386;N:41362,76,0,,,561606319,416363486,408486827,588324386,41362,SRX9785052,SRS7972285,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.9274,,0.16109,,0.74351,,0.57108,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62744,SRR13360750,SRX9785051,SRS7972284,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,soybean,soybean4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:soybeanCP replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CP4,CP4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cp4_S4_R1_001.fastq.gz,fastq,2913402316.0,38334241.0,cp4 S4 R1 001.fastq.gz,0:76 1:0,A:791131658;C:648321809;G:621173454;T:852725371;N:50024,76,0,,,791131658,648321809,621173454,852725371,50024,SRX9785051,SRS7972284,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93191,,0.1275,,0.7333,,0.55298,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62745,SRR13360751,SRX9785050,SRS7972283,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,soybean,soybean3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:soybeanCP replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CP3,CP3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cp3_S6_R1_001.fastq.gz,fastq,2648220684.0,34845009.0,cp3 S6 R1 001.fastq.gz,0:76 1:0,A:697194378;C:597685138;G:585283565;T:767999519;N:58084,76,0,,,697194378,597685138,585283565,767999519,58084,SRX9785050,SRS7972283,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93629,,0.1048,,0.7349,,0.54364,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62746,SRR13360752,SRX9785049,SRS7972282,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,soybean,soybean2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:soybeanCP replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CP2,CP2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cp2_S5_R1_001.fastq.gz,fastq,2339410872.0,30781722.0,cp2 S5 R1 001.fastq.gz,0:76 1:0,A:620056592;C:539298564;G:520500416;T:659503250;N:52050,76,0,,,620056592,539298564,520500416,659503250,52050,SRX9785049,SRS7972282,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93909,,0.0916,,0.70942,,0.51709,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62747,SRR13360753,SRX9785048,SRS7972281,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,soybean,soybean1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:soybeanCP replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CP1,CP1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cp1_S4_R1_001.fastq.gz,fastq,2271445592.0,29887442.0,cp1 S4 R1 001.fastq.gz,0:76 1:0,A:620595380;C:508382666;G:499686333;T:642731924;N:49289,76,0,,,620595380,508382666,499686333,642731924,49289,SRX9785048,SRS7972281,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93497,,0.1129,,0.71127,,0.50787,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62748,SRR13360754,SRX9785047,SRS7972280,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,control,control4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:controlCZ replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CZ4,CZ4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cz4_S1_R1_001.fastq.gz,fastq,2772612848.0,36481748.0,cz4 S1 R1 001.fastq.gz,0:76 1:0,A:753409223;C:625962831;G:590503241;T:802690640;N:46913,76,0,,,753409223,625962831,590503241,802690640,46913,SRX9785047,SRS7972280,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.92942,,0.15362,,0.62889,,0.51867,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62749,SRR13360755,SRX9785046,SRS7972279,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,control,control3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:controlCZ replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CZ3,CZ3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cz3_S3_R1_001.fastq.gz,fastq,2424287672.0,31898522.0,cz3 S3 R1 001.fastq.gz,0:76 1:0,A:629966430;C:571438897;G:558947157;T:663881953;N:53235,76,0,,,629966430,571438897,558947157,663881953,53235,SRX9785046,SRS7972279,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.94199,,0.04836,,0.72218,,0.48478,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62750,SRR13360756,SRX9785045,SRS7972278,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,macrogard,macrogard4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:macrogardPM replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,PM4,PM4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,pm4_S7_R1_001.fastq.gz,fastq,2901513560.0,38177810.0,pm4 S7 R1 001.fastq.gz,0:76 1:0,A:737189471;C:698799076;G:664901115;T:800575145;N:48753,76,0,,,737189471,698799076,664901115,800575145,48753,SRX9785045,SRS7972278,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.95173,,0.04367,,0.74089,,0.50116,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62751,SRR13360757,SRX9785044,SRS7972277,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,macrogard,macrogard3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:macrogardPM replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,PM3,PM3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,pm3_S9_R1_001.fastq.gz,fastq,3148390744.0,41426194.0,pm3 S9 R1 001.fastq.gz,0:76 1:0,A:834545372;C:725073648;G:704241250;T:884462724;N:67750,76,0,,,834545372,725073648,704241250,884462724,67750,SRX9785044,SRS7972277,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.94256,,0.09248,,0.72099,,0.52303,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62752,SRR13360758,SRX9785043,SRS7972276,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,control,control2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:controlCZ replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CZ2,CZ2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cz2_S2_R1_001.fastq,fastq,2207708116.0,29048791.0,cz2 S2 R1 001.fastq,0:76 1:0,A:614048827;C:488071931;G:474165427;T:631372646;N:49285,76,0,,,614048827,488071931,474165427,631372646,49285,SRX9785043,SRS7972276,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93271,,0.12538,,0.71733,,0.53166,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 62753,SRR13360759,SRX9785042,SRS7972275,SRP300470,PRJNA689790,Elucidating the effects of microbe derived glucans on the gut microbiota and the immune system of zebrafish,PRJNA689790,Other,We present a holistic picture of soybean induced changes in the intestine of juvenile zebrafish and the effect of yeast beta glucan through a transcriptomic approach. Dietary soybean altered the expression of specific GTP binding genes which are known to initiate inflammatory responses in the intestinal tissue. The intestinal barrier function appears to be affected by the modulation of mucin genes and genes linked to junction proteins. Furthermore the inclusion of soybean meal affected metabolic genes that are related to oxidative stress and genes related to lipid metabolism namely arl4ca and elovl6 in the intestinal tissue. Genes that counter inflammation were also upregulated in the soybean fed group. Our study also explains the plausible effect of yeast beta glucan on the intestinal barrier through regulation of immune responses and autophagy and strengthening of the mucosal barrier. Our findings indicate a subdued inflammation in juvenile zebrafish fed soybean meal and a strengthened mucosal barrier in the fish fed yeast beta glucan.,,,,control,control1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:50 days|dev stage:Juvenile|sex:not applicable|tissue:intestine|collected by:Saima Nord University|collection date:2019 11|treatment:controlCZ replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,CZ1,CZ1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP300470,,,cz1_S1_R1_001.fastq.gz,fastq,2264115696.0,29790996.0,cz1 S1 R1 001.fastq.gz,0:76 1:0,A:619936364;C:496981021;G:501038961;T:646109550;N:49800,76,0,,,619936364,496981021,501038961,646109550,49800,SRX9785042,SRS7972275,SRA1180489,Nord University|Faculty of Bioscience,Nord University,1,0.93737,,0.1307,,0.73003,,0.55416,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-01-05,Juvenile,Juvenile,Gut,Digestive System 66094,SRR15910149,SRX12200592,SRS10176883,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC4,HC4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_5_S5_R1_001.fastq.gz,fastq,1878287660.0,24877927.0,high 5 S5 R1 001.fastq.gz,0:75.50 1:0,A:476823620;C:439449647;G:429501262;T:532480837;N:32294,75,0,,,476823620,439449647,429501262,532480837,32294,SRX12200592,SRS10176883,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94553,,0.08147,,0.74582,,0.53024,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66095,SRR15910150,SRX12200591,SRS10176881,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC3,HC3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_4_S4_R1_001.fastq.gz,fastq,1888383069.0,25020396.0,high 4 S4 R1 001.fastq.gz,0:75.47 1:0,A:503511674;C:424269464;G:408669727;T:551898358;N:33846,75,0,,,503511674,424269464,408669727,551898358,33846,SRX12200591,SRS10176881,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93958,,0.1137,,0.74472,,0.52962,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66096,SRR15910151,SRX12200590,SRS10176882,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC2,HC2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_3_S6_R1_001.fastq.gz,fastq,2337788390.0,31029913.0,high 3 S6 R1 001.fastq.gz,0:75.34 1:0,A:586705132;C:555534330;G:547485592;T:645264200;N:2799136,75,0,,,586705132,555534330,547485592,645264200,2799136,SRX12200590,SRS10176882,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94651,,0.08395,,0.67872,,0.51496,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66097,SRR15910152,SRX12200589,SRS10176880,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC1,HC1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_1_S4_R1_001.fastq.gz,fastq,2390465384.0,31789893.0,high 1 S4 R1 001.fastq.gz,0:75.20 1:0,A:599574460;C:559752099;G:546456599;T:677639257;N:7042969,75,0,,,599574460,559752099,546456599,677639257,7042969,SRX12200589,SRS10176880,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94326,,0.09122,,0.73892,,0.52413,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66098,SRR15910153,SRX12200588,SRS10176878,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC6,LC6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_6_S3_R1_001.fastq.gz,fastq,1915028032.0,25430699.0,control 6 S3 R1 001.fastq.gz,0:75.30 1:0,A:502285547;C:435337245;G:424726824;T:549290123;N:3388293,75,0,,,502285547,435337245,424726824,549290123,3388293,SRX12200588,SRS10176878,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.92666,,0.1269,,0.74968,,0.54447,,73,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66099,SRR15910154,SRX12200587,SRS10176877,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC5,LC5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_5_S2_R1_001.fastq.gz,fastq,2327013759.0,30824465.0,control 5 S2 R1 001.fastq.gz,0:75.49 1:0,A:590955916;C:541856449;G:529786509;T:664374176;N:40709,75,0,,,590955916,541856449,529786509,664374176,40709,SRX12200587,SRS10176877,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94087,,0.09604,,0.74489,,0.53048,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66100,SRR15910155,SRX12200586,SRS10176879,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC4,LC4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_4_S1_R1_001.fastq.gz,fastq,1559648966.0,20660136.0,control 4 S1 R1 001.fastq.gz,0:75.49 1:0,A:398086562;C:359082782;G:356015350;T:446436847;N:27425,75,0,,,398086562,359082782,356015350,446436847,27425,SRX12200586,SRS10176879,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93707,,0.10017,,0.73464,,0.52715,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66101,SRR15910156,SRX12200585,SRS10176876,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS6,SS6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_6_S15_R1_001.fastq.gz,fastq,2431012086.0,32275098.0,sim 6 S15 R1 001.fastq.gz,0:75.32 1:0,A:647191218;C:551606901;G:529110857;T:703050921;N:52189,75,0,,,647191218,551606901,529110857,703050921,52189,SRX12200585,SRS10176876,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93331,,0.1165,,0.75268,,0.52753,,74,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66102,SRR15910157,SRX12200584,SRS10176875,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS5,SS5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_5_S14_R1_001.fastq.gz,fastq,1902101028.0,25201273.0,sim 5 S14 R1 001.fastq.gz,0:75.48 1:0,A:482206150;C:442300454;G:433280346;T:544280250;N:33828,75,0,,,482206150,442300454,433280346,544280250,33828,SRX12200584,SRS10176875,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93441,,0.09457,,0.72431,,0.53047,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66103,SRR15910158,SRX12200583,SRS10176874,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS4,SS4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_4_S13_R1_001.fastq.gz,fastq,1887931329.0,25021534.0,sim 4 S13 R1 001.fastq.gz,0:75.45 1:0,A:483691629;C:436592360;G:424733601;T:542880751;N:32988,75,0,,,483691629,436592360,424733601,542880751,32988,SRX12200583,SRS10176874,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.92435,,0.09393,,0.76039,,0.54018,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66104,SRR15910159,SRX12200582,SRS10176873,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS3,SS3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_3_S15_R1_001.fastq.gz,fastq,2278093448.0,30193199.0,sim 3 S15 R1 001.fastq.gz,0:75.45 1:0,A:575276152;C:529447047;G:521207330;T:650819961;N:1342958,75,0,,,575276152,529447047,521207330,650819961,1342958,SRX12200582,SRS10176873,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94093,,0.087,,0.73876,,0.53488,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66105,SRR15910160,SRX12200581,SRS10176872,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS2,SS2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_2_S14_R1_001.fastq.gz,fastq,2543469673.0,33741391.0,sim 2 S14 R1 001.fastq.gz,0:75.38 1:0,A:667279341;C:584937962;G:562098766;T:726456492;N:2697112,75,0,,,667279341,584937962,562098766,726456492,2697112,SRX12200581,SRS10176872,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93369,,0.11515,,0.72283,,0.51172,,74,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66106,SRR15910161,SRX12200580,SRS10176871,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Simvastatin,simvastatin 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:simvastatinSS replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,SS1,SS1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,sim_1_S13_R1_001.fastq.gz,fastq,1663748041.0,22080724.0,sim 1 S13 R1 001.fastq.gz,0:75.35 1:0,A:424766717;C:386318148;G:378172921;T:472190364;N:2299891,75,0,,,424766717,386318148,378172921,472190364,2299891,SRX12200580,SRS10176871,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93979,,0.08815,,0.74194,,0.52705,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66107,SRR15910162,SRX12200579,SRS10176870,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG6,OG6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_6_S12_R1_001.fastq.gz,fastq,1950238266.0,25861117.0,oat 6 S12 R1 001.fastq.gz,0:75.41 1:0,A:502273927;C:456918053;G:438368291;T:552640342;N:37653,75,0,,,502273927,456918053,438368291,552640342,37653,SRX12200579,SRS10176870,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93318,,0.08879,,0.74901,,0.54257,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66108,SRR15910163,SRX12200578,SRS10176869,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG5,OG5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_5_S11_R1_001.fastq.gz,fastq,1818990592.0,24148417.0,oat 5 S11 R1 001.fastq.gz,0:75.33 1:0,A:460795176;C:423681740;G:415715028;T:516509914;N:2288734,75,0,,,460795176,423681740,415715028,516509914,2288734,SRX12200578,SRS10176869,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93555,,0.09413,,0.73732,,0.54828,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66109,SRR15910164,SRX12200577,SRS10176868,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG4,OG4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_4_S10_R1_001.fastq.gz,fastq,1734135458.0,22972939.0,oat 4 S10 R1 001.fastq.gz,0:75.49 1:0,A:444047928;C:402248080;G:393083056;T:494726063;N:30331,75,0,,,444047928,402248080,393083056,494726063,30331,SRX12200577,SRS10176868,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93619,,0.09257,,0.75635,,0.54807,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66110,SRR15910165,SRX12200576,SRS10176867,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC3,LC3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_3_S3_R1_001.fastq.gz,fastq,2544827138.0,33810050.0,control 3 S3 R1 001.fastq.gz,0:75.27 1:0,A:649235065;C:587469807;G:574700155;T:728104191;N:5317920,75,0,,,649235065,587469807,574700155,728104191,5317920,SRX12200576,SRS10176867,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93445,,0.09084,,0.75471,,0.53891,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66111,SRR15910166,SRX12200575,SRS10176866,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG3,OG3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_3_S12_R1_001.fastq.gz,fastq,2676109047.0,35499063.0,oat 3 S12 R1 001.fastq.gz,0:75.39 1:0,A:668562223;C:624794464;G:608983398;T:770847910;N:2921052,75,0,,,668562223,624794464,608983398,770847910,2921052,SRX12200575,SRS10176866,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.95624,,0.07731,,0.78368,,0.5561,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66112,SRR15910167,SRX12200574,SRS10176865,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG2,OG2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_2_S11_R1_001.fastq.gz,fastq,1920714201.0,25464331.0,oat 2 S11 R1 001.fastq.gz,0:75.43 1:0,A:489513738;C:448024192;G:438816907;T:542892434;N:1466930,75,0,,,489513738,448024192,438816907,542892434,1466930,SRX12200574,SRS10176865,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94472,,0.08495,,0.73766,,0.54149,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66113,SRR15910168,SRX12200573,SRS10176864,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Oat glucan,oat 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:oatglucanOG replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,OG1,OG1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,oat_1_S10_R1_001.fastq.gz,fastq,2171014631.0,28803940.0,oat 1 S10 R1 001.fastq.gz,0:75.37 1:0,A:550611852;C:504793219;G:494893735;T:618143286;N:2572539,75,0,,,550611852,504793219,494893735,618143286,2572539,SRX12200573,SRS10176864,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94056,,0.089,,0.74142,,0.53823,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66114,SRR15910169,SRX12200572,SRS10176863,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 6,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate6|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG6,AG6,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_6_S12_R1_001.fastq.gz,fastq,2098747142.0,27807858.0,alga 6 S12 R1 001.fastq.gz,0:75.47 1:0,A:534561461;C:494167859;G:489052359;T:580927807;N:37656,75,0,,,534561461,494167859,489052359,580927807,37656,SRX12200572,SRS10176863,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94831,,0.08427,,0.73292,,0.52842,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66115,SRR15910170,SRX12200571,SRS10176862,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG5,AG5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_5_S11_R1_001.fastq.gz,fastq,1763481116.0,23363097.0,alga 5 S11 R1 001.fastq.gz,0:75.48 1:0,A:447338889;C:412970655;G:414050708;T:489089552;N:31312,75,0,,,447338889,412970655,414050708,489089552,31312,SRX12200571,SRS10176862,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94349,,0.08062,,0.739,,0.52999,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66116,SRR15910171,SRX12200570,SRS10176861,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 4,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate4|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG4,AG4,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_4_S10_R1_001.fastq.gz,fastq,1879826054.0,24897114.0,alga 4 S10 R1 001.fastq.gz,0:75.50 1:0,A:470066955;C:443235235;G:435473614;T:531017855;N:32395,75,0,,,470066955,443235235,435473614,531017855,32395,SRX12200570,SRS10176861,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94546,,0.09326,,0.65845,,0.52459,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66117,SRR15910172,SRX12200569,SRS10176858,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 3,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG3,AG3,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_3_S12_R1_001.fastq.gz,fastq,2171152050.0,28804773.0,alga 3 S12 R1 001.fastq.gz,0:75.37 1:0,A:577878327;C:486896436;G:474333381;T:629132016;N:2911890,75,0,,,577878327,486896436,474333381,629132016,2911890,SRX12200569,SRS10176858,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.9263,,0.13343,,0.74308,,0.54791,,74,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66118,SRR15910173,SRX12200568,SRS10176857,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG2,AG2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_2_S11_R1_001.fastq.gz,fastq,2667876981.0,35347747.0,alga 2 S11 R1 001.fastq.gz,0:75.48 1:0,A:608681566;C:714247949;G:658536241;T:684669115;N:1742110,75,0,,,608681566,714247949,658536241,684669115,1742110,SRX12200568,SRS10176857,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.9577,,0.13465,,0.77425,,0.60177,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66119,SRR15910174,SRX12200567,SRS10176859,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Algal glucan,alga 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,AG1,AG1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,alga_1_S10_R_001.fastq.gz,fastq,2947209259.0,39094391.0,alga 1 S10 R 001.fastq.gz,0:75.39 1:0,A:769302900;C:671912702;G:657628697;T:845634633;N:2730327,75,0,,,769302900,671912702,657628697,845634633,2730327,SRX12200567,SRS10176859,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93867,,0.08991,,0.74272,,0.53283,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66120,SRR15910175,SRX12200566,SRS10176855,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,High cholesterol,high 5,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:cholesterolHC replicate5|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,HC5,HC5,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,high_6_S6_R1_001.fastq.gz,fastq,1822201784.0,24137941.0,high 6 S6 R1 001.fastq.gz,0:75.49 1:0,A:461483337;C:426648920;G:419516035;T:514521907;N:31585,75,0,,,461483337,426648920,419516035,514521907,31585,SRX12200566,SRS10176855,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.94209,,0.09014,,0.74468,,0.50897,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66121,SRR15910176,SRX12200565,SRS10176860,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 2,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC2,LC2,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_2_S2_R1_001.fastq.gz,fastq,2450938234.0,32590616.0,control 2 S2 R1 001.fastq.gz,0:75.20 1:0,A:628466312;C:568472904;G:556931536;T:689980376;N:7087106,75,0,,,628466312,568472904,556931536,689980376,7087106,SRX12200565,SRS10176860,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.93782,,0.10577,,0.73699,,0.52,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System 66122,SRR15910177,SRX12200564,SRS10176856,SRP337313,PRJNA763718,Management of hypercholesterolemia through dietary beta glucans in a zebrafish model,PRJNA763718,Other,Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine.,,,,Control,control 1,,strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:controlLC replicate1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish,LC1,LC1,PCR enrichment of adaptor ligated DNA,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 550,,SRP337313,,,control_1_S1_R1_001.fastq.gz,fastq,2784628452.0,37009570.0,control 1 S1 R1 001.fastq.gz,0:75.24 1:0,A:700349148;C:654385631;G:634389883;T:789658848;N:5844942,75,0,,,700349148,654385631,634389883,789658848,5844942,SRX12200564,SRS10176856,SRA1295228,Nord University|Faculty of Biosciences and Aquaculture,Nord University,1,0.9386,,0.08609,,0.73829,,0.53049,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,unknown,bulk,unknown,unknown,,Norway,2021-09-16,Adult,Adult,Gut,Digestive System