rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 57271,SRR12577970,SRX9064853,SRS7314026,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ctrl 3 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 6'|BioSampleModel:Model organism or animal,,,,,,,,,Ctrl 3 zebrafish,Ctrl 3 zebrafish,Ctrl 3 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ctrl_3_1.fq.gz,fastq,1061227250.0,21224545.0,Ctrl 3 1.fq.gz,0:50,A:286099732;C:241244619;G:246876865;T:287006034;N:0,50,,,,286099732,241244619,246876865,287006034,0,SRX9064853,SRS7314026,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94563,,0.10453,,0.70552,,0.46655,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 57272,SRR12577971,SRX9064852,SRS7314025,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ctrl 2 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 5 prime|BioSampleModel:Model organism or animal,,,,,,,,,Ctrl 2 zebrafish,Ctrl 2 zebrafish,Ctrl 2 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ctrl_2_1.fq.gz,fastq,1058116700.0,21162334.0,Ctrl 2 1.fq.gz,0:50,A:287223905;C:238707750;G:244987559;T:287197486;N:0,50,,,,287223905,238707750,244987559,287197486,0,SRX9064852,SRS7314025,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94534,,0.11174,,0.70104,,0.47946,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 57273,SRR12577972,SRX9064851,SRS7314024,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ctrl 1 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 4'|BioSampleModel:Model organism or animal,,,,,,,,,Ctrl 1 zebrafish,Ctrl 1 zebrafish,Ctrl 1 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ctrl_1_1.fq.gz,fastq,1065919600.0,21318392.0,Ctrl 1 1.fq.gz,0:50,A:295920720;C:242146677;G:242962633;T:284889570;N:0,50,,,,295920720,242146677,242962633,284889570,0,SRX9064851,SRS7314024,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94228,,0.15007,,0.68028,,0.46845,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 57274,SRR12577973,SRX9064850,SRS7314023,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ator STS 3 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 3 prime|BioSampleModel:Model organism or animal,,,,,,,,,Ator STS 3 zebrafish,Ator STS 3 zebrafish,Ator STS 3 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ator_STS_3_1.fq.gz,fastq,1059717350.0,21194347.0,Ator STS 3 1.fq.gz,0:50,A:287279590;C:239708340;G:245740313;T:286989107;N:0,50,,,,287279590,239708340,245740313,286989107,0,SRX9064850,SRS7314023,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94345,,0.10458,,0.69686,,0.4755,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 57275,SRR12577974,SRX9064849,SRS7314022,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ator STS 2 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 2'|BioSampleModel:Model organism or animal,,,,,,,,,Ator STS 2 zebrafish,Ator STS 2 zebrafish,Ator STS 2 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ator_STS_2_1.fq.gz,fastq,1057950250.0,21159005.0,Ator STS 2 1.fq.gz,0:50,A:286256354;C:238832868;G:244078781;T:288782247;N:0,50,,,,286256354,238832868,244078781,288782247,0,SRX9064849,SRS7314022,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94307,,0.10974,,0.69631,,0.4703,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 57276,SRR12577975,SRX9064848,SRS7314021,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ator STS 1 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 1'|BioSampleModel:Model organism or animal,,,,,,,,,Ator STS 1 zebrafish,Ator STS 1 zebrafish,Ator STS 1 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ator_STS_1_1.fq.gz,fastq,1070136700.0,21402734.0,Ator STS 1 1.fq.gz,0:50,A:297601042;C:243837062;G:243583907;T:285114689;N:0,50,,,,297601042,243837062,243583907,285114689,0,SRX9064848,SRS7314021,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94665,,0.12219,,0.67953,,0.46393,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 57277,SRR12577976,SRX9064847,SRS7314020,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ator 3 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 3 prime|BioSampleModel:Model organism or animal,,,,,,,,,Ator 3 zebrafish,Ator 3 zebrafish,Ator 3 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ator_3_1.fq.gz,fastq,1058031900.0,21160638.0,Ator 3 1.fq.gz,0:50,A:285399527;C:239993172;G:245571467;T:287067734;N:0,50,,,,285399527,239993172,245571467,287067734,0,SRX9064847,SRS7314020,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94536,,0.11052,,0.6957,,0.48195,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 57278,SRR12577977,SRX9064846,SRS7314019,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ator 2 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 2'|BioSampleModel:Model organism or animal,,,,,,,,,Ator 2 zebrafish,Ator 2 zebrafish,Ator 2 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ator_2_1.fq.gz,fastq,1059618400.0,21192368.0,Ator 2 1.fq.gz,0:50,A:283429136;C:243023053;G:248904818;T:284261393;N:0,50,,,,283429136,243023053,248904818,284261393,0,SRX9064846,SRS7314019,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94743,,0.09493,,0.70218,,0.475,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 57279,SRR12577978,SRX9064845,SRS7314018,SRP279881,PRJNA612371,Danio rerio strain:TU Raw sequence reads,PRJNA612371,Whole Genome Sequencing,Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage.,,,,,Ator 1 zebrafish,,strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 1'|BioSampleModel:Model organism or animal,,,,,,,,,Ator 1 zebrafish,Ator 1 zebrafish,Ator 1 zebrafish,transcriptome,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP279881,,,Ator_1_1.fq.gz,fastq,1075695950.0,21513919.0,Ator 1 1.fq.gz,0:50,A:297768036;C:244470267;G:243768925;T:289688722;N:0,50,,,,297768036,244470267,243768925,289688722,0,SRX9064845,SRS7314018,SRA1120721,Shanghai University of Traditional Chinese Medicine|Longhua Hospital,Shanghai University of Traditional Chinese Medicine,1,0.94468,,0.13183,,0.68363,,0.46288,,50,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2020-09-03,Hatching,Embryo,Head,Nervous System 66964,SRR16965143,SRX13156592,SRS11088496,SRP346547,PRJNA780808,transcriptomics of carbendazim and enrofloxacin on zebrafish embryos,PRJNA780808,Other,reveal a interaction between ENF and CAR on metabolic regulation during development,,,,,1mix,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:10 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio :mixture group,mix 1,mix 1,96hpf zebrafish larvae mixture group,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346547,,,mix2_1_2.fq.gz,fastq,3313823250.0,22092155.0,mix2 1 2.fq.gz,0:0 1:150,A:895726357;C:763402657;G:745765587;T:908922686;N:5963,0,150,,,895726357,763402657,745765587,908922686,5963,SRX13156592,SRS11088496,SRA1329868,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.93604,,0.10794,,0.65587,,0.47956,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-17,Larval,Larval,Undetermined,Undetermined 66965,SRR16965144,SRX13156591,SRS11088495,SRP346547,PRJNA780808,transcriptomics of carbendazim and enrofloxacin on zebrafish embryos,PRJNA780808,Other,reveal a interaction between ENF and CAR on metabolic regulation during development,,,,,3enf,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:9 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio :enrofloxacin group,enf 3,enf 3,96hpf zebrafish larvae enrofloxacin group,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346547,,,enf_3.fq.gz,fastq,3304972350.0,22033149.0,enf 3.fq.gz,0:150 1:0,A:899427202;C:745999066;G:759917309;T:899625137;N:3636,150,0,,,899427202,745999066,759917309,899625137,3636,SRX13156591,SRS11088495,SRA1329868,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.94353,,0.10795,,0.66212,,0.47773,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-17,Larval,Larval,Undetermined,Undetermined 66967,SRR16965146,SRX13156589,SRS11088492,SRP346547,PRJNA780808,transcriptomics of carbendazim and enrofloxacin on zebrafish embryos,PRJNA780808,Other,reveal a interaction between ENF and CAR on metabolic regulation during development,,,,,3car,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio :carbendazim group,car 3,car 3,96hpf zebrafish larvae carbendazim group,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346547,,,car_3_1.fq.gz,fastq,3335622150.0,22237481.0,car 3 1.fq.gz,0:150 1:0,A:853229671;C:807433601;G:820478265;T:854475092;N:5521,150,0,,,853229671,807433601,820478265,854475092,5521,SRX13156589,SRS11088492,SRA1329868,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.96567,,0.03093,,0.70997,,0.47751,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-17,Larval,Larval,Undetermined,Undetermined 66968,SRR16965147,SRX13156588,SRS11088494,SRP346547,PRJNA780808,transcriptomics of carbendazim and enrofloxacin on zebrafish embryos,PRJNA780808,Other,reveal a interaction between ENF and CAR on metabolic regulation during development,,,,,1car,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:4 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio :carbendazim group,car 1,car 1,96hpf zebrafish larvae carbendazim group,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346547,,,car_1_1.fq.gz,fastq,3378056400.0,22520376.0,car 1 1.fq.gz,0:150 1:0,A:886009175;C:795203398;G:809447342;T:887390300;N:6185,150,0,,,886009175,795203398,809447342,887390300,6185,SRX13156588,SRS11088494,SRA1329868,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.95701,,0.05956,,0.67836,,0.4884,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-17,Larval,Larval,Undetermined,Undetermined 66969,SRR16965148,SRX13156587,SRS11088491,SRP346547,PRJNA780808,transcriptomics of carbendazim and enrofloxacin on zebrafish embryos,PRJNA780808,Other,reveal a interaction between ENF and CAR on metabolic regulation during development,,,,,3con,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:3 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio :control group,con 3,con 3,96hpf zebrafish larvae control group,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346547,,,con_3_2.fq.gz,fastq,3359054500.0,33590545.0,con 3 2.fq.gz,0:100 1:0,A:914533337;C:755914202;G:773676246;T:914930715;N:0,100,0,,,914533337,755914202,773676246,914930715,0,SRX13156587,SRS11088491,SRA1329868,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.95076,,0.10478,,0.65646,,0.49594,,100,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-17,Larval,Larval,Undetermined,Undetermined 66970,SRR16965149,SRX13156586,SRS11088489,SRP346547,PRJNA780808,transcriptomics of carbendazim and enrofloxacin on zebrafish embryos,PRJNA780808,Other,reveal a interaction between ENF and CAR on metabolic regulation during development,,,,,3mix,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:12 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio :mixture group,mix 3,mix 3,96hpf zebrafish larvae mixture group,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346547,,,mix2_3_1.fq.gz,fastq,3362094000.0,33620940.0,mix2 3 1.fq.gz,0:100 1:0,A:916124379;C:755910593;G:768246534;T:921812494;N:0,100,0,,,916124379,755910593,768246534,921812494,0,SRX13156586,SRS11088489,SRA1329868,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.94894,,0.10416,,0.65342,,0.49475,,100,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-17,Larval,Larval,Undetermined,Undetermined 66972,SRR16965151,SRX13156584,SRS11088488,SRP346547,PRJNA780808,transcriptomics of carbendazim and enrofloxacin on zebrafish embryos,PRJNA780808,Other,reveal a interaction between ENF and CAR on metabolic regulation during development,,,,,2con,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:2 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio :control group,con 2,con 2,96hpf zebrafish larvae control group,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346547,,loader:fastq load.py,con_2_2.fq,fastq,3324287550.0,22161917.0,con 2 2.fq.gz,0:150,A:848766265;C:812477645;G:798995610;T:864040426;N:7604,150,,,,848766265,812477645,798995610,864040426,7604,SRX13156584,SRS11088488,SRA1329868,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.95546,,0.03524,,0.70027,,0.48649,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-22,Larval,Larval,Undetermined,Undetermined 66973,SRR16965152,SRX13156583,SRS11088487,SRP346547,PRJNA780808,transcriptomics of carbendazim and enrofloxacin on zebrafish embryos,PRJNA780808,Other,reveal a interaction between ENF and CAR on metabolic regulation during development,,,,,1con,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:1 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio :control group,con 1,con 1,96hpf zebrafish larvae control group,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346547,,loader:fastq load.py,con_1_1.fq,fastq,3330724500.0,22204830.0,con 1 1.fq.gz,0:150,A:896812508;C:762246067;G:774062043;T:897597946;N:5936,150,,,,896812508,762246067,774062043,897597946,5936,SRX13156583,SRS11088487,SRA1329868,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.94926,,0.08711,,0.66789,,0.48628,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-22,Larval,Larval,Undetermined,Undetermined 66975,SRR16959539,SRX13151073,SRS11083379,SRP346418,PRJNA780940,transcriptomics of difenoconazole and dimethomorph on zebrafish embryos,PRJNA780940,Other,Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.,,,,,2dim,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:8 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio : dimethomorph group,dim 2,dim 2,The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular.,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346418,,,dim_2_1.fq.gz,fastq,3344257500.0,22295050.0,dim 2 1.fq.gz,0:150 1:0,A:895700119;C:767415749;G:784994320;T:896143585;N:3727,150,0,,,895700119,767415749,784994320,896143585,3727,SRX13151073,SRS11083379,SRA1330365,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.9497,,0.08418,,0.66352,,0.49341,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-16,Larval,Larval,Undetermined,Undetermined 66976,SRR16959540,SRX13151072,SRS11083378,SRP346418,PRJNA780940,transcriptomics of difenoconazole and dimethomorph on zebrafish embryos,PRJNA780940,Other,Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.,,,,,1dim,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:7 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio : dimethomorph group,dim 1,dim 1,The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular.,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346418,,,dim_1_1.fq.gz,fastq,3318812250.0,22125415.0,dim 1 1.fq.gz,0:150 1:0,A:908481293;C:742787936;G:755930542;T:911608821;N:3658,150,0,,,908481293,742787936,755930542,911608821,3658,SRX13151072,SRS11083378,SRA1330365,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.94211,,0.11824,,0.65344,,0.47716,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-16,Larval,Larval,Undetermined,Undetermined 66977,SRR16959541,SRX13151071,SRS11083377,SRP346418,PRJNA780940,transcriptomics of difenoconazole and dimethomorph on zebrafish embryos,PRJNA780940,Other,Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.,,,,,3dif,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio : difenoconazole group,dif 3,dif 3,The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular.,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346418,,,dif_3_1.fq.gz,fastq,3318453000.0,22123020.0,dif 3 1.fq.gz,0:150 1:0,A:893975607;C:758489621;G:772391705;T:893592335;N:3732,150,0,,,893975607,758489621,772391705,893592335,3732,SRX13151071,SRS11083377,SRA1330365,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.94838,,0.08393,,0.66543,,0.4818,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-16,Larval,Larval,Undetermined,Undetermined 66978,SRR16959542,SRX13151070,SRS11083376,SRP346418,PRJNA780940,transcriptomics of difenoconazole and dimethomorph on zebrafish embryos,PRJNA780940,Other,Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.,,,,,2dif,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:5 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio : difenoconazole group,dif 2,dif 2,The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular.,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346418,,,dif_2_2.fq.gz,fastq,3304972350.0,22033149.0,dif 2 2.fq.gz,0:0 1:150,A:890456734;C:764917766;G:744757536;T:904833787;N:6527,0,150,,,890456734,764917766,744757536,904833787,6527,SRX13151070,SRS11083376,SRA1330365,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.93514,,0.107,,0.6633,,0.47599,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-16,Larval,Larval,Undetermined,Undetermined 66979,SRR16959543,SRX13151069,SRS11083375,SRP346418,PRJNA780940,transcriptomics of difenoconazole and dimethomorph on zebrafish embryos,PRJNA780940,Other,Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.,,,,,1dif,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:4 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio : difenoconazole group,dif 1,dif 1,The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular.,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346418,,,dif_1_1.fq.gz,fastq,3295443900.0,21969626.0,dif 1 1.fq.gz,0:150 1:0,A:887936493;C:751636494;G:765508757;T:890358629;N:3527,150,0,,,887936493,751636494,765508757,890358629,3527,SRX13151069,SRS11083375,SRA1330365,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.94623,,0.09138,,0.66478,,0.4828,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-16,Larval,Larval,Undetermined,Undetermined 66980,SRR16959544,SRX13151068,SRS11083374,SRP346418,PRJNA780940,transcriptomics of difenoconazole and dimethomorph on zebrafish embryos,PRJNA780940,Other,Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.,,,,,3+con,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:3 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio : control group,con 3,con 3,The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular.,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346418,,,con_3_1.fq.gz,fastq,3306406800.0,22042712.0,con 3 1.fq.gz,0:150 1:0,A:872740236;C:773482184;G:788334419;T:871846539;N:3422,150,0,,,872740236,773482184,788334419,871846539,3422,SRX13151068,SRS11083374,SRA1330365,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.95458,,0.06103,,0.67409,,0.48439,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-16,Larval,Larval,Undetermined,Undetermined 66982,SRR16959546,SRX13151066,SRS11083372,SRP346418,PRJNA780940,transcriptomics of difenoconazole and dimethomorph on zebrafish embryos,PRJNA780940,Other,Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.,,,,,2+con,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:2 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio : control group,con 2,con 2,The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular.,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346418,,,con_2_2.fq.gz,fastq,3324287550.0,22161917.0,con 2 2.fq.gz,0:0 1:150,A:848766265;C:812477645;G:798995610;T:864040426;N:7604,0,150,,,848766265,812477645,798995610,864040426,7604,SRX13151066,SRS11083372,SRA1330365,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.95548,,0.03524,,0.70021,,0.4867,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-16,Larval,Larval,Undetermined,Undetermined 66983,SRR16959547,SRX13151065,SRS11083371,SRP346418,PRJNA780940,transcriptomics of difenoconazole and dimethomorph on zebrafish embryos,PRJNA780940,Other,Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.,,,,,1+con,,strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:1 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Danio rerio : control group,con 1,con 1,The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular.,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,BGISEQ,BGISEQ-500,,SRP346418,,,con_1_1.fq.gz,fastq,3330724500.0,22204830.0,con 1 1.fq.gz,0:150 1:0,A:896812508;C:762246067;G:774062043;T:897597946;N:5936,150,0,,,896812508,762246067,774062043,897597946,5936,SRX13151065,SRS11083371,SRA1330365,China Agricultural University|College of animal science and technology,China Agricultural University,1,0.94925,,0.08711,,0.66772,,0.48635,,150,,B,,usable mapping rate,bgi,bgi,unknown,poly_a,unknown,bulk,unknown,unknown,,China,2021-11-16,Larval,Larval,Undetermined,Undetermined