rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
38,DRR408248,DRX393854,DRS407179,DRP012042,PRJDB14275,Zebrafish EN/ENCDC RNA seq,DRP012042,Transcriptome Analysis,A project to find differential expressed genes between enteric neurons ENs and enteric neural crest derived cells ENCDCs in larval zebrafish gut. We dissected guts of transgenic line TgSAGFFLF217B; uas:gfp for ENs and Tgsox10:cre; EF1alpha:loxP gfp loxP dsred for ENCDCs and isolated GFP+ ENs and dsRed+ ENCDCs. Three duplicates for each of ENs and ENCDCs are prepared. Libraries for NGS are prepared using SMART Seq V4 Ultra Low Input RNA Kit.,,,zebrafish 5 day DsRed positive enteric neural crest derived cells replicate 3,zebrafish ENCDC replicate 3,SAMD00529468,,sample name:zebrafish ENCDC replicate 3|biological replicate:enteric neural crest derived cells 3|strain:Tgsox10:cre; EF3alpha:loxP gfp loxP dsred,,,,,,,,,NextSeq 550 paired end sequencing of SAMD00529468,DRX393854,190326ENvsNC N706 5day;NeuralCrestDerivedCell;rep3,1,1,,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,NextSeq 550,1600Application ReadForward11Application ReadReverse81,DRP012042,NextSeq 550 paired end sequencing of SAMD00529468,,,,3803721121.0,24526633.0,DRR408248,0:77.54 1:77.54,A:999106107;C:897663781;G:921501114;T:979486853;N:5963266,77,77,,,999106107,897663781,921501114,979486853,5963266,DRX393854,DRS407179,DRA014886,"NIBB|NIBB core research facilities, National Institute for Basic Biology",University of Hyogo,,,,,,,,,,,,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Japan,2024-09-22,Undetermined,Larval,Brain,Nervous System
39,DRR408247,DRX393853,DRS407178,DRP012042,PRJDB14275,Zebrafish EN/ENCDC RNA seq,DRP012042,Transcriptome Analysis,A project to find differential expressed genes between enteric neurons ENs and enteric neural crest derived cells ENCDCs in larval zebrafish gut. We dissected guts of transgenic line TgSAGFFLF217B; uas:gfp for ENs and Tgsox10:cre; EF1alpha:loxP gfp loxP dsred for ENCDCs and isolated GFP+ ENs and dsRed+ ENCDCs. Three duplicates for each of ENs and ENCDCs are prepared. Libraries for NGS are prepared using SMART Seq V4 Ultra Low Input RNA Kit.,,,zebrafish 5 day DsRed positive enteric neural crest derived cells replicate 2,zebrafish ENCDC replicate 2,SAMD00529467,,sample name:zebrafish ENCDC replicate 2|biological replicate:enteric neural crest derived cells 2|strain:Tgsox10:cre; EF2alpha:loxP gfp loxP dsred,,,,,,,,,NextSeq 550 paired end sequencing of SAMD00529467,DRX393853,190326ENvsNC N705 5day;NeuralCrestDerivedCell;rep2,1,1,,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,NextSeq 550,1600Application ReadForward11Application ReadReverse81,DRP012042,NextSeq 550 paired end sequencing of SAMD00529467,,,,3436798274.0,22156202.0,DRR408247,0:77.56 1:77.56,A:900848174;C:812031426;G:832960423;T:885671203;N:5287048,77,77,,,900848174,812031426,832960423,885671203,5287048,DRX393853,DRS407178,DRA014886,"NIBB|NIBB core research facilities, National Institute for Basic Biology",University of Hyogo,,,,,,,,,,,,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Japan,2024-09-22,Undetermined,Larval,Brain,Nervous System
40,DRR408246,DRX393852,DRS407177,DRP012042,PRJDB14275,Zebrafish EN/ENCDC RNA seq,DRP012042,Transcriptome Analysis,A project to find differential expressed genes between enteric neurons ENs and enteric neural crest derived cells ENCDCs in larval zebrafish gut. We dissected guts of transgenic line TgSAGFFLF217B; uas:gfp for ENs and Tgsox10:cre; EF1alpha:loxP gfp loxP dsred for ENCDCs and isolated GFP+ ENs and dsRed+ ENCDCs. Three duplicates for each of ENs and ENCDCs are prepared. Libraries for NGS are prepared using SMART Seq V4 Ultra Low Input RNA Kit.,,,zebrafish 5 day DsRed positive enteric neural crest derived cells replicate 1,zebrafish ENCDC replicate 1,SAMD00529466,,sample name:zebrafish ENCDC replicate 1|biological replicate:enteric neural crest derived cells 1|strain:Tgsox10:cre; EF1alpha:loxP gfp loxP dsred,,,,,,,,,NextSeq 550 paired end sequencing of SAMD00529466,DRX393852,190326ENvsNC N704 5day;NeuralCrestDerivedCell;rep1,1,1,,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,NextSeq 550,1600Application ReadForward11Application ReadReverse81,DRP012042,NextSeq 550 paired end sequencing of SAMD00529466,,,,3582073512.0,23135170.0,DRR408246,0:77.41 1:77.42,A:943152815;C:841972211;G:863627245;T:927361159;N:5960082,77,77,,,943152815,841972211,863627245,927361159,5960082,DRX393852,DRS407177,DRA014886,"NIBB|NIBB core research facilities, National Institute for Basic Biology",University of Hyogo,,,,,,,,,,,,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Japan,2024-09-22,Undetermined,Larval,Brain,Nervous System
44,DRR668250,DRX648352,DRS458865,DRP012880,PRJDB18466,Comparison of spinal cord regeneration capacity in zebrafish and medaka,PRJDB18466,Other,Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability.,,pubmed:40278963,Zebrafish 2 weeks post spinal cord injury replicate 3,Zebrafish 2wpi 3,SAMD00799623,,sample name:Zebrafish 2wpi 3|biological replicate:3|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 25|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord,,,,,,,,,DNBSEQ G400 paired end sequencing of SAMD00799623,DRX648352,RNA seq of spinal cord in zebrafish at 2wpi injured 3,1,Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,DNBSEQ,DNBSEQ-G400,,DRP012880,DNBSEQ G400 paired end sequencing of SAMD00799623,,,,14782516800.0,73912584.0,DRR668250,0:100 1:100,A:4058090278;C:3335994894;G:3323563782;T:4062467903;N:2399943,100,100,,,4058090278,3335994894,3323563782,4062467903,2399943,DRX648352,DRS458865,DRA020617,Osaka University,Osaka University,,,,,,,,,,,,B,B,biological fallback assumption,bgi,bgi,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2025-05-12,Adult,Adult,Spinal Cord,Nervous System
45,DRR668249,DRX648351,DRS458864,DRP012880,PRJDB18466,Comparison of spinal cord regeneration capacity in zebrafish and medaka,PRJDB18466,Other,Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability.,,pubmed:40278963,Zebrafish 2 weeks post spinal cord injury replicate 2,Zebrafish 2wpi 2,SAMD00799622,,sample name:Zebrafish 2wpi 2|biological replicate:2|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 14|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord,,,,,,,,,DNBSEQ G400 paired end sequencing of SAMD00799622,DRX648351,RNA seq of spinal cord in zebrafish at 2wpi injured 2,1,Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,DNBSEQ,DNBSEQ-G400,,DRP012880,DNBSEQ G400 paired end sequencing of SAMD00799622,,,,13687641800.0,68438209.0,DRR668249,0:100 1:100,A:3759784620;C:3087398782;G:3083881581;T:3754378915;N:2197902,100,100,,,3759784620,3087398782,3083881581,3754378915,2197902,DRX648351,DRS458864,DRA020617,Osaka University,Osaka University,,,,,,,,,,,,B,B,biological fallback assumption,bgi,bgi,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2025-05-12,Adult,Adult,Spinal Cord,Nervous System
46,DRR668248,DRX648350,DRS458863,DRP012880,PRJDB18466,Comparison of spinal cord regeneration capacity in zebrafish and medaka,PRJDB18466,Other,Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability.,,pubmed:40278963,Zebrafish 2 weeks post spinal cord injury replicate 1,Zebrafish 2wpi 1,SAMD00799621,,sample name:Zebrafish 2wpi 1|biological replicate:1|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 14|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord,,,,,,,,,DNBSEQ G400 paired end sequencing of SAMD00799621,DRX648350,RNA seq of spinal cord in zebrafish at 2wpi injured 1,1,Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,DNBSEQ,DNBSEQ-G400,,DRP012880,DNBSEQ G400 paired end sequencing of SAMD00799621,,,,16376197200.0,81880986.0,DRR668248,0:100 1:100,A:4485868844;C:3700974430;G:3710833937;T:4475827800;N:2692189,100,100,,,4485868844,3700974430,3710833937,4475827800,2692189,DRX648350,DRS458863,DRA020617,Osaka University,Osaka University,,,,,,,,,,,,B,B,biological fallback assumption,bgi,bgi,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2025-05-12,Adult,Adult,Spinal Cord,Nervous System
47,DRR668247,DRX648349,DRS458862,DRP012880,PRJDB18466,Comparison of spinal cord regeneration capacity in zebrafish and medaka,PRJDB18466,Other,Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability.,,pubmed:40278963,Zebrafish Intact biological replicate 3,Zebrafish Control 3,SAMD00799620,,sample name:Zebrafish Control 3|biological replicate:3|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 21|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord,,,,,,,,,DNBSEQ G400 paired end sequencing of SAMD00799620,DRX648349,RNA seq of spinal cord in zebrafish at 0wpi control 3,1,Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,DNBSEQ,DNBSEQ-G400,,DRP012880,DNBSEQ G400 paired end sequencing of SAMD00799620,,,,13377538600.0,66887693.0,DRR668247,0:100 1:100,A:3725064764;C:2973653932;G:2980883214;T:3695767890;N:2168800,100,100,,,3725064764,2973653932,2980883214,3695767890,2168800,DRX648349,DRS458862,DRA020617,Osaka University,Osaka University,,,,,,,,,,,,B,B,biological fallback assumption,bgi,bgi,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2025-05-12,Adult,Adult,Spinal Cord,Nervous System
48,DRR668246,DRX648348,DRS458861,DRP012880,PRJDB18466,Comparison of spinal cord regeneration capacity in zebrafish and medaka,PRJDB18466,Other,Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability.,,pubmed:40278963,Zebrafish Intact biological replicate 2,Zebrafish Control 2,SAMD00799619,,sample name:Zebrafish Control 2|biological replicate:2|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 21|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord,,,,,,,,,DNBSEQ G400 paired end sequencing of SAMD00799619,DRX648348,RNA seq of spinal cord in zebrafish at 0wpi control 2,1,Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,DNBSEQ,DNBSEQ-G400,,DRP012880,DNBSEQ G400 paired end sequencing of SAMD00799619,,,,14971411400.0,74857057.0,DRR668246,0:100 1:100,A:4160326445;C:3329083037;G:3329123314;T:4150453700;N:2424904,100,100,,,4160326445,3329083037,3329123314,4150453700,2424904,DRX648348,DRS458861,DRA020617,Osaka University,Osaka University,,,,,,,,,,,,B,B,biological fallback assumption,bgi,bgi,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2025-05-12,Adult,Adult,Spinal Cord,Nervous System
49,DRR668245,DRX648347,DRS458860,DRP012880,PRJDB18466,Comparison of spinal cord regeneration capacity in zebrafish and medaka,PRJDB18466,Other,Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability.,,pubmed:40278963,Zebrafish Intact biological replicate 1,Zebrafish Control 1,SAMD00799618,,sample name:Zebrafish Control 1|biological replicate:1|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2024 05 04|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord,,,,,,,,,DNBSEQ G400 paired end sequencing of SAMD00799618,DRX648347,RNA seq of spinal cord in zebrafish at 0wpi control 1,1,Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,DNBSEQ,DNBSEQ-G400,,DRP012880,DNBSEQ G400 paired end sequencing of SAMD00799618,,,,13912523800.0,69562619.0,DRR668245,0:100 1:100,A:3888902049;C:3079617959;G:3075111814;T:3866655202;N:2236776,100,100,,,3888902049,3079617959,3075111814,3866655202,2236776,DRX648347,DRS458860,DRA020617,Osaka University,Osaka University,,,,,,,,,,,,B,B,biological fallback assumption,bgi,bgi,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2025-05-12,Adult,Adult,Spinal Cord,Nervous System
131,DRR189373,DRX179838,DRS200446,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Dissociated cells from telencephalon of wild type adult zebrafish 3,SAMD00182216,,sample name:Cell Tel 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182216,DRX179838,Cell Tel 3,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182216,,,,4142772200.0,20713861.0,DRR189373,0:100 1:100,A:1185533233;C:877739936;G:877025820;T:1202041821;N:431390,100,100,,,1185533233,877739936,877025820,1202041821,431390,DRX179838,DRS200446,DRA008855,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.9018,0.90073,0.24953,0.25062,0.67884,0.67957,0.49957,0.5402,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
132,DRR189372,DRX179837,DRS200445,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Dissociated cells from telencephalon of wild type adult zebrafish 2,SAMD00182215,,sample name:Cell Tel 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182215,DRX179837,Cell Tel 2,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182215,,,,5233184600.0,26165923.0,DRR189372,0:100 1:100,A:1522745973;C:1082449570;G:1076728796;T:1550682585;N:577676,100,100,,,1522745973,1082449570,1076728796,1550682585,577676,DRX179837,DRS200445,DRA008855,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89481,0.89419,0.27137,0.27018,0.67639,0.67671,0.54949,0.55323,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
133,DRR189371,DRX179836,DRS200444,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Dissociated cells from telencephalon of wild type adult zebrafish 1,SAMD00182214,,sample name:Cell Tel 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182214,DRX179836,Cell Tel 1,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182214,,,,4407578800.0,22037894.0,DRR189371,0:100 1:100,A:1270948410;C:923917605;G:922122984;T:1290126238;N:463563,100,100,,,1270948410,923917605,922122984,1290126238,463563,DRX179836,DRS200444,DRA008855,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.90057,0.90117,0.24092,0.24074,0.67598,0.67775,0.53184,0.53433,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
134,DRR189370,DRX179835,DRS200415,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 5,SAMD00182213,,sample name:h62A GFP Tel 5|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182213,DRX179835,h62A GFP Tel 5,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182213,,,,2904332400.0,14521662.0,DRR189370,0:100 1:100,A:821602205;C:620206517;G:623738781;T:838474359;N:310538,100,100,,,821602205,620206517,623738781,838474359,310538,DRX179835,DRS200415,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91925,0.91942,0.14636,0.14584,0.71127,0.71429,0.62818,0.40804,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
135,DRR189369,DRX179834,DRS200414,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 4,SAMD00182212,,sample name:h62A GFP Tel 4|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182212,DRX179834,h62A GFP Tel 4,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182212,,,,4670374000.0,23351870.0,DRR189369,0:100 1:100,A:1294304230;C:1030286884;G:1032728119;T:1312561064;N:493703,100,100,,,1294304230,1030286884,1032728119,1312561064,493703,DRX179834,DRS200414,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.92587,0.92725,0.12279,0.12312,0.71056,0.71293,0.57854,0.57882,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
136,DRR189368,DRX179833,DRS200413,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 3,SAMD00182211,,sample name:h62A GFP Tel 3|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182211,DRX179833,h62A GFP Tel 3,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182211,,,,11487645000.0,57438225.0,DRR189368,0:100 1:100,A:3127119396;C:2611288030;G:2547219656;T:3194997425;N:7020493,100,100,,,3127119396,2611288030,2547219656,3194997425,7020493,DRX179833,DRS200413,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91389,0.91371,0.11188,0.11336,0.74576,0.74639,0.56209,0.56027,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
137,DRR189367,DRX179832,DRS200412,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 2,SAMD00182210,,sample name:h62A GFP Tel 2|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182210,DRX179832,h62A GFP Tel 2,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182210,,,,13536257200.0,67681286.0,DRR189367,0:100 1:100,A:3723068804;C:3049411151;G:2951685691;T:3803778575;N:8312979,100,100,,,3723068804,3049411151,2951685691,3803778575,8312979,DRX179832,DRS200412,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.9136,0.91493,0.14102,0.14249,0.70534,0.70569,0.58385,0.58171,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
138,DRR189366,DRX179831,DRS200411,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of h62A;UAS:GFP adult zebrafish 1,SAMD00182209,,sample name:h62A GFP Tel 1|genotype:hspGFF62A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182209,DRX179831,h62A GFP Tel 1,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182209,,,,13057029600.0,65285148.0,DRR189366,0:100 1:100,A:3609997372;C:2912841795;G:2821782510;T:3704302917;N:8105006,100,100,,,3609997372,2912841795,2821782510,3704302917,8105006,DRX179831,DRS200411,DRA008854,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.90766,0.90786,0.13813,0.14007,0.73677,0.73777,0.55675,0.55743,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
139,DRR189365,DRX179830,DRS200432,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of SAGFF120A;UAS:GFP adult zebrafish 4,SAMD00182208,,sample name:120A GFP Tel 4|genotype:SAGFF120A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00182208,DRX179830,120A GFP Tel 4,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2000 paired end sequencing of SAMD00182208,,,,4105132200.0,20525661.0,DRR189365,0:100 1:100,A:1171500567;C:874901685;G:872257219;T:1186025546;N:447183,100,100,,,1171500567,874901685,872257219,1186025546,447183,DRX179830,DRS200432,DRA008853,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91106,0.90924,0.18729,0.18688,0.69406,0.69589,0.51697,0.51253,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
140,DRR189364,DRX179829,DRS200431,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of SAGFF120A;UAS:GFP adult zebrafish 3,SAMD00182207,,sample name:120A GFP Tel 3|genotype:SAGFF120A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182207,DRX179829,120A GFP Tel 3,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182207,,,,5062007400.0,25310037.0,DRR189364,0:100 1:100,A:1425414609;C:1105334563;G:1072448536;T:1458726402;N:83290,100,100,,,1425414609,1105334563,1072448536,1458726402,83290,DRX179829,DRS200431,DRA008853,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89598,0.89539,0.2645,0.2668,0.69591,0.6971,0.49901,0.49932,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
141,DRR189363,DRX179828,DRS200430,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of SAGFF120A;UAS:GFP adult zebrafish 2,SAMD00182206,,sample name:120A GFP Tel 2|genotype:SAGFF120A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182206,DRX179828,120A GFP Tel 2,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182206,,,,4148886200.0,20744431.0,DRR189363,0:100 1:100,A:1121946535;C:959659540;G:901840216;T:1165367793;N:72116,100,100,,,1121946535,959659540,901840216,1165367793,72116,DRX179828,DRS200430,DRA008853,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89808,0.89803,0.14106,0.14229,0.74057,0.74073,0.58127,0.58258,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
142,DRR189362,DRX179827,DRS200429,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form telencephalon of SAGFF120A;UAS:GFP adult zebrafish 1,SAMD00182205,,sample name:120A GFP Tel 1|genotype:SAGFF120A;UAS:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182205,DRX179827,120A GFP Tel 1,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182205,,,,5621230800.0,28106154.0,DRR189362,0:100 1:100,A:1511319423;C:1299516107;G:1236814355;T:1573482053;N:98862,100,100,,,1511319423,1299516107,1236814355,1573482053,98862,DRX179827,DRS200429,DRA008853,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.90171,0.90205,0.11967,0.12059,0.74083,0.74113,0.61779,0.61682,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
143,DRR189361,DRX179826,DRS200425,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form whole brain of HuC:GFP adult zebrafish 4,SAMD00182204,,sample name:HuC GFP WB 4|genotype:HuC:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182204,DRX179826,HuC GFP WB 4,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182204,,,,3641515200.0,18207576.0,DRR189361,0:100 1:100,A:1038686472;C:777269450;G:777179582;T:1047994354;N:385342,100,100,,,1038686472,777269450,777179582,1047994354,385342,DRX179826,DRS200425,DRA008852,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91246,0.9127,0.18566,0.18547,0.70201,0.7024,0.47049,0.47102,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
144,DRR189360,DRX179825,DRS200424,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form whole brain of HuC:GFP adult zebrafish 3,SAMD00182203,,sample name:HuC GFP WB 3|genotype:HuC:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182203,DRX179825,HuC GFP WB 3,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182203,,,,4692211000.0,23461055.0,DRR189360,0:100 1:100,A:1310884816;C:1036187295;G:997846600;T:1347212024;N:80265,100,100,,,1310884816,1036187295,997846600,1347212024,80265,DRX179825,DRS200424,DRA008852,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89626,0.89739,0.18174,0.18338,0.72443,0.72588,0.4577,0.46684,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
145,DRR189359,DRX179824,DRS200423,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form whole brain of HuC:GFP adult zebrafish 2,SAMD00182202,,sample name:HuC GFP WB 2|genotype:HuC:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182202,DRX179824,HuC GFP WB 2,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182202,,,,7514071000.0,37570355.0,DRR189359,0:100 1:100,A:2098251885;C:1661407212;G:1597423444;T:2156861774;N:126685,100,100,,,2098251885,1661407212,1597423444,2156861774,126685,DRX179824,DRS200423,DRA008852,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89548,0.89691,0.26231,0.26473,0.70358,0.70471,0.4732,0.4753,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
146,DRR189358,DRX179823,DRS200422,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,GFP cells form whole brain of HuC:GFP adult zebrafish 1,SAMD00182201,,sample name:HuC GFP WB 1|genotype:HuC:GFP|tissue:brain,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00182201,DRX179823,HuC GFP WB 1,1,The cDNA was amplified using SMARTer v4. Sequence libraries were produced from cDNA using Nextera XT DNA library kit Illumina.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00182201,,,,5175824800.0,25879124.0,DRR189358,0:100 1:100,A:1424688686;C:1174545239;G:1128653980;T:1447848363;N:88532,100,100,,,1424688686,1174545239,1128653980,1447848363,88532,DRX179823,DRS200422,DRA008852,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.91906,0.91958,0.20879,0.21088,0.74787,0.74876,0.45243,0.45221,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2021-08-08,Adult,Adult,Brain,Nervous System
147,DRR051067,DRX045959,DRS057267,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,These cells are from the same fish as h62A GFP plus Tel,GFP cells from telencephalon of hspGFF62A;UAS:GFP transgenic zebrafish,SAMD00044994,,sample name:h62A GFP minus Tel|tissue type:brain|genotype:hspGFF62A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044994,DRX045959,h62A GFP minus Tel,1,cDNA synthesis : clontech SMARTer v3 > Library prep : Illumina Nextera XT DNA Library Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044994,,,,17913873600.0,89569368.0,DRR051067,0:100 1:100,A:5009629866;C:3957379257;G:3797879751;T:5144366288;N:4618438,100,100,,,5009629866,3957379257,3797879751,5144366288,4618438,DRX045959,DRS057267,DRA004277,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89075,0.89087,0.23044,0.23295,0.69493,0.69769,0.5366,0.54852,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2018-01-06,Undetermined,Undetermined,Brain,Nervous System
148,DRR051066,DRX045958,DRS057275,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Collected from 40 adult fish by using FACS,GFP+ cells from telencephalon of hspGFF62A;UAS:GFP transgenic zebrafish,SAMD00044995,,sample name:h62A GFP plus Tel|tissue type:brain|genotype:hspGFF62A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044995,DRX045958,h62A GFP plus Tel,1,cDNA synthesis : clontech SMARTer v3 > Library prep : Illumina Nextera XT DNA Library Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044995,,,,17421256000.0,87106280.0,DRR051066,0:100 1:100,A:4859368314;C:3846634012;G:3717526030;T:4993260256;N:4467388,100,100,,,4859368314,3846634012,3717526030,4993260256,4467388,DRX045958,DRS057275,DRA004276,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.88127,0.88114,0.22941,0.23155,0.72161,0.72437,0.49284,0.49449,100,100,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,nextera,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
149,DRR051065,DRX045957,DRS057272,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Telencephalon from adult zebrafrish 30 min post light and electrical shock association in non trace Two Way Active Avoidance conditioning,CS+US telencephalon 30 min post TWAA,SAMD00044990,,sample name:CS+US Tel 30|tissue type:brain|genotype:WT,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044990,DRX045957,CS+US Tel 30,1,Illumina Truseq RNA Library Prep Kit v2,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044990,,,,20239329200.0,101196646.0,DRR051065,0:100 1:100,A:5347169722;C:4777492672;G:4750401220;T:5361888239;N:2377347,100,100,,,5347169722,4777492672,4750401220,5361888239,2377347,DRX045957,DRS057272,DRA004275,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.93576,0.93353,0.13979,0.14019,0.70043,0.70203,0.48989,0.49214,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
150,DRR051064,DRX045956,DRS057265,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Telencephalon from adult zebrafrish 30 min post light stimulation in Two Way Active Avoidance coditioning,CS telencephalon 30 min post TWAA,SAMD00044991,,sample name:CS Tel 30|tissue type:brain|genotype:WT,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044991,DRX045956,CS Tel 30,1,Illumina Truseq RNA Library Prep Kit v2,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000Application ReadForward11Application ReadReverse101,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044991,,,,29268319600.0,146341598.0,DRR051064,0:100 1:100,A:7777792429;C:6878969107;G:6819275179;T:7788795270;N:3487615,100,100,,,7777792429,6878969107,6819275179,7788795270,3487615,DRX045956,DRS057265,DRA004274,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.93646,0.93664,0.14051,0.14191,0.70199,0.70374,0.49256,0.49684,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
151,DRR051063,DRX045955,DRS057266,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,These cells are from the same fish as 120A GFP plus Tel,GFP cells from telencephalon of SAGFFLF120A;UAS:GFP transgenic zebrafish,SAMD00044987,,sample name:120A GFP minus Tel|tissue type:brain|genotype:SAGFFLF120A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044987,DRX045955,120A GFP minus Tel,1,cDNA synthesis : clontech SMARTer v2 > Library prep : Illumina TrunSeq DNA Sample Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2020Application ReadForward11Application ReadReverse102,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044987,,,,15411400120.0,76294060.0,DRR051063,0:101 1:101,A:4583085286;C:3082552426;G:3134669086;T:4606055524;N:5037798,101,101,,,4583085286,3082552426,3134669086,4606055524,5037798,DRX045955,DRS057266,DRA004273,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.89184,0.8842,0.30246,0.30334,0.69232,0.705,0.52824,0.51492,101,101,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,smarter,bulk,unknown,unknown,,Japan,2018-01-06,Undetermined,Undetermined,Brain,Nervous System
152,DRR051062,DRX045954,DRS057271,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Collected from 40 adult fish by using FACS,GFP+ cells from telencephalon of SAGFFLF120A;UAS:GFP transgenic zebrafish,SAMD00044988,,sample name:120A GFP plus Tel|tissue type:brain|genotype:SAGFFLF120A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044988,DRX045954,120A GFP plus Tel,1,cDNA synthesis : clontech SMARTer v2 > Library prep : Illumina TrunSeq DNA Sample Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2020Application ReadForward11Application ReadReverse102,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044988,,,,9916242014.0,49090307.0,DRR051062,0:101 1:101,A:2991626000;C:1935418605;G:1974202781;T:3011783158;N:3211470,101,101,,,2991626000,1935418605,1974202781,3011783158,3211470,DRX045954,DRS057271,DRA004272,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.87779,0.86382,0.2948,0.28993,0.72809,0.74075,0.50317,0.49169,101,101,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,smarter,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
153,DRR051061,DRX045953,DRS057274,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Collected from 10 adult fish by using FACS,GFP+ cells from whole brain of SAGFFLF231A;UAS:GFP transgenic zebrafish,SAMD00044989,,sample name:231A GFP plus WB|tissue type:brain|genotype:SAGFFLF231A;UAS:GFP,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044989,DRX045953,231A GFP plus WB,1,cDNA synthesis : clontech SMARTer v2 > Library prep : Illumina TrunSeq DNA Sample Preparation Kits,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2020Application ReadForward11Application ReadReverse102,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044989,,,,27230654000.0,136153270.0,DRR051061,0:101 1:99,A:7929214734;C:5506861409;G:5563593280;T:8059057191;N:171927386,101,99,,,7929214734,5506861409,5563593280,8059057191,171927386,DRX045953,DRS057274,DRA004271,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.88462,0.88282,0.26215,0.26067,0.72468,0.73555,0.48369,0.48285,101,99,B,B,biological fallback assumption,illumina,hiseq_era,full_length,cdna_unspecified,smarter,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
154,DRR051060,DRX045952,DRS057269,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Telencephalon of adult zebrafrish barin,zebrafihsh telencephalon,SAMD00044992,,sample name:Tel|tissue type:brain|genotype:WT,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044992,DRX045952,Tel,1,Illumina Truseq RNA Library Prep Kit v2,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2020Application ReadForward11Application ReadReverse102,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044992,,,,20006128076.0,99040238.0,DRR051060,0:101 1:101,A:5393556813;C:4623783008;G:4592812230;T:5384415659;N:11560366,101,101,,,5393556813,4623783008,4592812230,5384415659,11560366,DRX045952,DRS057269,DRA004270,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.94215,0.94001,0.16398,0.16585,0.69331,0.69479,0.4872,0.48516,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
155,DRR051059,DRX045951,DRS057270,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,Whole brain of adult zebrafish,zebrafish whole brain,SAMD00044993,,sample name:WB|tissue type:brain|genotype:WT,,,,,,,,,Illumina HiSeq 2500 paired end sequencing of SAMD00044993,DRX045951,WB,1,Illumina Truseq RNA Library Prep Kit v2,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2500,2020Application ReadForward11Application ReadReverse102,DRP003977,Illumina HiSeq 2500 paired end sequencing of SAMD00044993,,,,25283347776.0,125165088.0,DRR051059,0:101 1:101,A:6845696813;C:5810492697;G:5775241119;T:6837142940;N:14774207,101,101,,,6845696813,5810492697,5775241119,6837142940,14774207,DRX045951,DRS057270,DRA004269,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),2,0.94203,0.94067,0.15107,0.15158,0.68651,0.6882,0.49596,0.49589,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-01-06,Adult,Adult,Brain,Nervous System
260,DRR162481,DRX153100,DRS083161,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 39 mpf zebrafish replicate5,SAMD00152429,,sample name:b39 5|age:39 month|biological replicate:5|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152429,DRX153100,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152429,,,,1252728200.0,6263641.0,DRR162481,0:100 1:100,A:367502128;C:257734618;G:257260262;T:368202459;N:2028733,100,100,,,367502128,257734618,257260262,368202459,2028733,DRX153100,DRS083161,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92142,0.8899,0.17636,0.1685,0.70686,0.7166,0.53271,0.54366,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
261,DRR162480,DRX153099,DRS083160,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 39 mpf zebrafish replicate4,SAMD00152428,,sample name:b39 4|age:39 month|biological replicate:4|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152428,DRX153099,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152428,,,,1426772800.0,7133864.0,DRR162480,0:100 1:100,A:408404333;C:303657580;G:303201065;T:408996815;N:2513007,100,100,,,408404333,303657580,303201065,408996815,2513007,DRX153099,DRS083160,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92435,0.89379,0.16127,0.15446,0.69844,0.70554,0.53646,0.533,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
262,DRR162479,DRX153098,DRS083159,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 39 mpf zebrafish replicate3,SAMD00152427,,sample name:b39 3|age:39 month|biological replicate:3|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152427,DRX153098,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152427,,,,1197809800.0,5989049.0,DRR162479,0:100 1:100,A:348955835;C:249141949;G:248747996;T:348970452;N:1993568,100,100,,,348955835,249141949,248747996,348970452,1993568,DRX153098,DRS083159,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92402,0.89389,0.1731,0.1668,0.70938,0.72892,0.55061,0.56711,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
263,DRR162478,DRX153097,DRS083158,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 39 mpf zebrafish replicate2,SAMD00152426,,sample name:b39 2|age:39 month|biological replicate:2|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152426,DRX153097,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152426,,,,1389632200.0,6948161.0,DRR162478,0:100 1:100,A:397337261;C:295934915;G:295794427;T:398240347;N:2325250,100,100,,,397337261,295934915,295794427,398240347,2325250,DRX153097,DRS083158,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92803,0.89621,0.15624,0.14998,0.69988,0.70985,0.54263,0.53655,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
264,DRR162477,DRX153096,DRS083157,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 39 mpf zebrafish replicate1,SAMD00152425,,sample name:b39 1|age:39 month|biological replicate:1|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152425,DRX153096,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152425,,,,1413538400.0,7067692.0,DRR162477,0:100 1:100,A:427890787;C:277522619;G:278488081;T:427755965;N:1880948,100,100,,,427890787,277522619,278488081,427755965,1880948,DRX153096,DRS083157,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90967,0.88004,0.20536,0.19647,0.71384,0.72021,0.55413,0.55769,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
265,DRR162476,DRX153095,DRS083156,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 16 mpf zebrafish replicate5,SAMD00152424,,sample name:b16 5|age:16 month|biological replicate:5|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152424,DRX153095,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152424,,,,1256635000.0,6283175.0,DRR162476,0:100 1:100,A:385448452;C:241878013;G:242164401;T:385408510;N:1735624,100,100,,,385448452,241878013,242164401,385408510,1735624,DRX153095,DRS083156,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91032,0.87693,0.19953,0.19064,0.72575,0.73474,0.49199,0.5657,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
266,DRR162475,DRX153094,DRS083155,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 16 mpf zebrafish replicate4,SAMD00152423,,sample name:b16 4|age:16 month|biological replicate:4|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152423,DRX153094,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152423,,,,2577820600.0,12889103.0,DRR162475,0:100 1:100,A:783031561;C:503484597;G:503899732;T:784003178;N:3401532,100,100,,,783031561,503484597,503899732,784003178,3401532,DRX153094,DRS083155,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91098,0.87948,0.20945,0.19993,0.71758,0.72543,0.56796,0.56453,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
267,DRR162474,DRX153093,DRS083154,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 16 mpf zebrafish replicate3,SAMD00152422,,sample name:b16 3|age:16 month|biological replicate:3|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152422,DRX153093,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152422,,,,1147257600.0,5736288.0,DRR162474,0:100 1:100,A:357829214;C:214920802;G:215124134;T:357870811;N:1512639,100,100,,,357829214,214920802,215124134,357870811,1512639,DRX153093,DRS083154,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90822,0.88184,0.20468,0.19612,0.73992,0.74627,0.58395,0.50711,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
268,DRR162473,DRX153092,DRS083153,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 16 mpf zebrafish replicate2,SAMD00152421,,sample name:b16 2|age:16 month|biological replicate:2|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152421,DRX153092,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152421,,,,1399740200.0,6998701.0,DRR162473,0:100 1:100,A:420492615;C:278464854;G:278362101;T:420657546;N:1763084,100,100,,,420492615,278464854,278362101,420657546,1763084,DRX153092,DRS083153,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91795,0.88458,0.19015,0.18122,0.71374,0.72318,0.55157,0.55554,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
269,DRR162472,DRX153091,DRS083152,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 16 mpf zebrafish replicate1,SAMD00152420,,sample name:b16 1|age:16 month|biological replicate:1|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152420,DRX153091,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152420,,,,1109512400.0,5547562.0,DRR162472,0:100 1:100,A:336800225;C:217086705;G:217195789;T:336929233;N:1500448,100,100,,,336800225,217086705,217195789,336929233,1500448,DRX153091,DRS083152,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91025,0.87734,0.20046,0.19197,0.71774,0.72482,0.52937,0.56111,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
270,DRR162471,DRX153090,DRS083151,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 7 mpf zebrafish replicate5,SAMD00152419,,sample name:b07 5|age:7 month|biological replicate:5|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152419,DRX153090,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152419,,,,1472475400.0,7362377.0,DRR162471,0:100 1:100,A:448100356;C:287211180;G:287652290;T:447581010;N:1930564,100,100,,,448100356,287211180,287652290,447581010,1930564,DRX153090,DRS083151,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90584,0.87079,0.21738,0.2083,0.71415,0.7344,0.5518,0.55812,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
271,DRR162470,DRX153089,DRS083150,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 7 mpf zebrafish replicate4,SAMD00152418,,sample name:b07 4|age:7 month|biological replicate:4|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152418,DRX153089,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152418,,,,1173234000.0,5866170.0,DRR162470,0:100 1:100,A:349147253;C:236479543;G:237153294;T:348938811;N:1515099,100,100,,,349147253,236479543,237153294,348938811,1515099,DRX153089,DRS083150,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91933,0.88973,0.18681,0.17878,0.71108,0.71867,0.5448,0.55274,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
272,DRR162469,DRX153088,DRS083149,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 7 mpf zebrafish replicate3,SAMD00152417,,sample name:b07 3|age:7 month|biological replicate:3|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152417,DRX153088,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152417,,,,1322055600.0,6610278.0,DRR162469,0:100 1:100,A:387777992;C:270907178;G:273067585;T:387904272;N:2398573,100,100,,,387777992,270907178,273067585,387904272,2398573,DRX153088,DRS083149,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91753,0.88469,0.17775,0.17007,0.70656,0.71685,0.5466,0.54528,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
273,DRR162468,DRX153087,DRS083148,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 7 mpf zebrafish replicate2,SAMD00152416,,sample name:b07 2|age:7 month|biological replicate:2|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152416,DRX153087,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152416,,,,1160349000.0,5801745.0,DRR162468,0:100 1:100,A:350211581;C:228387129;G:229679563;T:349919152;N:2151575,100,100,,,350211581,228387129,229679563,349919152,2151575,DRX153087,DRS083148,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91146,0.87717,0.20422,0.19494,0.7083,0.72082,0.55642,0.56386,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
274,DRR162467,DRX153086,DRS083147,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 7 mpf zebrafish replicate1,SAMD00152415,,sample name:b07 1|age:7 month|biological replicate:1|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152415,DRX153086,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152415,,,,1162314600.0,5811573.0,DRR162467,0:100 1:100,A:344932586;C:233867698;G:235450342;T:345967852;N:2096122,100,100,,,344932586,233867698,235450342,345967852,2096122,DRX153086,DRS083147,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.91102,0.88248,0.1897,0.18307,0.70786,0.71687,0.55008,0.54646,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Adult,Adult,Brain,Nervous System
275,DRR162466,DRX153085,DRS083146,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 2 mpf zebrafish replicate5,SAMD00152414,,sample name:b02 5|age:2 month|biological replicate:5|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152414,DRX153085,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152414,,,,1184455800.0,5922279.0,DRR162466,0:100 1:100,A:357035323;C:233685447;G:235595745;T:356085837;N:2053448,100,100,,,357035323,233685447,235595745,356085837,2053448,DRX153085,DRS083146,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90819,0.87146,0.20659,0.19751,0.71246,0.72251,0.52523,0.52531,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
276,DRR162465,DRX153084,DRS083145,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 2 mpf zebrafish replicate4,SAMD00152413,,sample name:b02 4|age:2 month|biological replicate:4|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152413,DRX153084,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152413,,,,1389141000.0,6945705.0,DRR162465,0:100 1:100,A:423029010;C:270113394;G:270981952;T:422602360;N:2414284,100,100,,,423029010,270113394,270981952,422602360,2414284,DRX153084,DRS083145,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90961,0.87739,0.21147,0.20152,0.71492,0.72563,0.53639,0.53779,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
277,DRR162464,DRX153083,DRS083144,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 2 mpf zebrafish replicate3,SAMD00152412,,sample name:b02 3|age:2 month|biological replicate:3|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152412,DRX153083,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152412,,,,2322459000.0,11612295.0,DRR162464,0:100 1:100,A:701085106;C:457451297;G:460424165;T:699185460;N:4312972,100,100,,,701085106,457451297,460424165,699185460,4312972,DRX153083,DRS083144,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.92479,0.89382,0.16108,0.1547,0.73312,0.74121,0.55089,0.55507,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
278,DRR162463,DRX153082,DRS083143,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 2 mpf zebrafish replicate2,SAMD00152411,,sample name:b02 2|age:2 month|biological replicate:2|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152411,DRX153082,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152411,,,,1188406400.0,5942032.0,DRR162463,0:100 1:100,A:354575190;C:238508166;G:239587004;T:353623510;N:2112530,100,100,,,354575190,238508166,239587004,353623510,2112530,DRX153082,DRS083143,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.90922,0.8787,0.20709,0.2009,0.71614,0.7362,0.52768,0.53049,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
279,DRR162462,DRX153081,DRS083142,DRP004696,PRJDB7713,Age associated transcriptome analysis in 5 tissues of zebrafish,DRP004696,Transcriptome Analysis,We performed transcriptome analysis for brain gill heart liver and muscle from 2 month 7 month 16 month and 39 mpf zebrafish. We analyzed age associated gene expression pattern in zebrafish and compared with similar public transcriptome data of rat Yu et al. 2014.,,,,brain sample from 2 mpf zebrafish replicate1,SAMD00152410,,sample name:b02 1|age:2 month|biological replicate:1|tissue:brain,,,,,,,,,Illumina HiSeq 2000 paired end sequencing of SAMD00152410,DRX153081,1,1,Illumina TruSeq Stranded mRNA HT Kit,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,DRP004696,Illumina HiSeq 2000 paired end sequencing of SAMD00152410,,,,1033492200.0,5167461.0,DRR162462,0:100 1:100,A:310135217;C:205335529;G:207428012;T:308760225;N:1833217,100,100,,,310135217,205335529,207428012,308760225,1833217,DRX153081,DRS083142,DRA007711,UT-AQUA|Laboratory of Aquatic Molecular Biology and Biotechnology,"Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo",2,0.9176,0.8829,0.18651,0.17848,0.72099,0.73119,0.54307,0.54697,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Japan,2018-12-24,Juvenile,Juvenile,Brain,Nervous System
7950,ERR015567,ERX005930,ERS000089,ERP000263,PRJEB2208,Zebrafish gene three prime end pull down for genome annotation,E-MTAB-308,Transcriptome Analysis,,,,RNA extracted from male adult zebrafish head,,SAMEA708835,SC,ENA FIRST PUBLIC:2010 02 26T10:44:13Z|ENA LAST UPDATE:2018 03 08T15:51:30Z|External Id:SAMEA708835|INSDC center name:SC|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:51:30Z|INSDC status:public|Submitter Id:ZF male head sample1|common name:zebrafish|sample name:ZF male head sample1|scientific name:Danio rerio|sex:male|strain:Tuebingen,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation,E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish male head dpf three prime pull down paired end 250 to 300 bp insert,Zebrafish adult male head mRNA three prime end,Zebrafish gene three prime end pull down for genome annotation,20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl. RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin magnetic beads. post washing by pull down on a magnet the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB. post further washing the double strand cDNA was released from the beads with BpmI NEB. The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.,Experimental Factor: DEVELOPMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:head|Experimental Factor: SEX:male,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000263,Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation,ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16,3444_7.srf,srf,1384012896.0,9105348.0,E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish male head dpf three prime pull down paired end 250 to 300 bp insert,0:76 1:76,A:369912216;C:306963547;G:292249178;T:392053840;N:22834115,76,76,,,369912216,306963547,292249178,392053840,22834115,ERX005930,ERS000089,ERA010603,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.95932,0.96084,0.20719,0.21321,0.73959,0.74763,0.47227,0.48033,76,76,B,B,biological fallback assumption,illumina,early_illumina,3prime,other,unknown,bulk,unknown,unknown,,United Kingdom,2010-02-26,Adult,Adult,Head,Nervous System
8059,ERR022480,ERX008923,ERS000088,ERP000400,PRJEB2333,Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer,E-MTAB-434,Other,,,,,ZF female head sample1,SAMEA708836,Wellcome Sanger Institute,Alias:ZF female head sample1|Description:RNA extracted from female adult zebrafish head|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:24:37Z|INSDC status:public|SRA accession:ERS000088|Sample Name:ERS000088|Sex:female|Strain:Tuebingen|Title:Danio rerio,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer,E MTAB 434:sequencing of Zebrafish adult female head,RNA from Zebrafish adult female head,Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer,Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at 70 C. Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down. The libraries have fragment size of 150 to 200 bp.,Experimental Factor: DEVELPOMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:head,FL-cDNA,TRANSCRIPTOMIC,unspecified,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000400,Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer,ENA FIRST PUBLIC:2011 03 10|ENA LAST UPDATE:2018 11 16,2719_5.srf,srf,1622590272.0,15023984.0,E MTAB 434:2719 5.srf,0:54 1:54,A:426435544;C:373743071;G:387589492;T:432086510;N:2735655,54,54,,,426435544,373743071,387589492,432086510,2735655,ERX008923,ERS000088,ERA015179,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.94624,0.94338,0.17949,0.17965,0.66454,0.66872,0.51097,0.51377,54,54,B,B,biological fallback assumption,illumina,early_illumina,unknown,poly_a,unknown,bulk,unknown,unknown,,United Kingdom,2010-02-26,Adult,Adult,Head,Nervous System
8060,ERR022481,ERX008923,ERS000088,ERP000400,PRJEB2333,Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer,E-MTAB-434,Other,,,,,ZF female head sample1,SAMEA708836,Wellcome Sanger Institute,Alias:ZF female head sample1|Description:RNA extracted from female adult zebrafish head|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:24:37Z|INSDC status:public|SRA accession:ERS000088|Sample Name:ERS000088|Sex:female|Strain:Tuebingen|Title:Danio rerio,,,,,,,,,Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer,E MTAB 434:sequencing of Zebrafish adult female head,RNA from Zebrafish adult female head,Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer,Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at 70 C. Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down. The libraries have fragment size of 150 to 200 bp.,Experimental Factor: DEVELPOMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:head,FL-cDNA,TRANSCRIPTOMIC,unspecified,PAIRED,ILLUMINA,Illumina Genome Analyzer II,,ERP000400,Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer,ENA FIRST PUBLIC:2011 03 10|ENA LAST UPDATE:2018 11 16,2719_6.srf,srf,1693460736.0,15680192.0,E MTAB 434:2719 6.srf,0:54 1:54,A:445666325;C:389360280;G:403789644;T:451529226;N:3115261,54,54,,,445666325,389360280,403789644,451529226,3115261,ERX008923,ERS000088,ERA015179,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.94579,0.94429,0.17895,0.17923,0.66864,0.67164,0.51788,0.51083,54,54,B,B,biological fallback assumption,illumina,early_illumina,unknown,poly_a,unknown,bulk,unknown,unknown,,United Kingdom,2010-02-26,Adult,Adult,Head,Nervous System
8064,ERR035545,ERX013540,ERS017861,ERP000447,PRJEB2368,Sanger zebrafish sequencing,E-MTAB-460,Other,,,,Protocols: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at 70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Zebrafish adult brain,SAMEA782568,Wellcome Sanger Institute,ENA first public:2011 02 03|ENA last update:2018 03 08|External Id:SAMEA782568|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2011 02 03T12:40:41Z|INSDC last update:2018 03 08T15:25:22Z|INSDC status:public|StrainOrLine:Singapore|Submitter Id:E MTAB 460:Zebrafish adult brain|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|organism part:brain|sample name:E MTAB 460:Zebrafish adult brain|sex:mixed,,,,,,,,,Sanger zebrafish sequencing,E MTAB 460 part2:5625 1,ZFbrain 2 RNA 1523492,Sanger zebrafish sequencing,Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at 70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was enriched for polyA+ RNA by 2 rounds of polyA pull down with magnetic beads and included a DNase treatment between the 2 rounds. RNA was chemically fragmented LiCl precipitated reverse transcribed with random primers a second strand synthesized and made into a standard Illumina library with a fragment size of 250 to 300 bp.,Experimental Factor: ORGANISM PART:brain,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina Genome Analyzer II,1600Application ReadForward11Technical ReadReverse772Application ReadReverse85,ERP000447,Illumina Genome Analyzer II paired end sequencing; Sanger zebrafish sequencing,ENA FIRST PUBLIC:2011 06 14|ENA LAST UPDATE:2018 11 16,5625_1.srf,srf,4576566720.0,28603542.0,E MTAB 460 part2:5625 1.srf,0:76 1:8 2:76,A:1227184716;C:942715631;G:951021478;T:1219839320;N:6977239,76,8,76,,1227184716,942715631,951021478,1219839320,6977239,ERX013540,ERS017861,ERA033503,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.86503,0.86182,0.22392,0.22256,0.69664,0.69865,0.51454,0.51606,76,76,B,B,biological fallback assumption,illumina,early_illumina,unknown,poly_a,unknown,bulk,unknown,unknown,,United Kingdom,2011-02-03,Adult,Adult,Brain,Nervous System
8069,ERR023147,ERX009449,ERS017861,ERP000447,PRJEB2368,Sanger zebrafish sequencing,E-MTAB-460,Other,,,,Protocols: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at 70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Zebrafish adult brain,SAMEA782568,Wellcome Sanger Institute,ENA first public:2011 02 03|ENA last update:2018 03 08|External Id:SAMEA782568|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2011 02 03T12:40:41Z|INSDC last update:2018 03 08T15:25:22Z|INSDC status:public|StrainOrLine:Singapore|Submitter Id:E MTAB 460:Zebrafish adult brain|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|organism part:brain|sample name:E MTAB 460:Zebrafish adult brain|sex:mixed,,,,,,,,,Sanger zebrafish sequencing,E MTAB 460:3537 7,RNA from Zebrafish adult brain,Sanger zebrafish sequencing,Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at 70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Experimental Factor: ORGANISM PART:brain,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina Genome Analyzer II,1520Application ReadForward11Application ReadReverse77,ERP000447,Illumina Genome Analyzer II paired end sequencing; Sanger zebrafish sequencing,ENA FIRST PUBLIC:2011 02 03|ENA LAST UPDATE:2018 11 16,3537_7.srf,srf,3047469128.0,20049139.0,E MTAB 460:3537 7.srf,0:76 1:76,A:951431181;C:561974807;G:565451314;T:964940357;N:3671469,76,76,,,951431181,561974807,565451314,964940357,3671469,ERX009449,ERS017861,ERA015648,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.89672,0.89537,0.31092,0.31152,0.75724,0.7583,0.56639,0.56663,76,76,B,B,biological fallback assumption,illumina,early_illumina,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2011-02-03,Adult,Adult,Brain,Nervous System
8070,ERR023144,ERX009448,ERS017861,ERP000447,PRJEB2368,Sanger zebrafish sequencing,E-MTAB-460,Other,,,,Protocols: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at 70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Zebrafish adult brain,SAMEA782568,Wellcome Sanger Institute,ENA first public:2011 02 03|ENA last update:2018 03 08|External Id:SAMEA782568|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2011 02 03T12:40:41Z|INSDC last update:2018 03 08T15:25:22Z|INSDC status:public|StrainOrLine:Singapore|Submitter Id:E MTAB 460:Zebrafish adult brain|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|organism part:brain|sample name:E MTAB 460:Zebrafish adult brain|sex:mixed,,,,,,,,,Sanger zebrafish sequencing,E MTAB 460:3212 6,RNA from Zebrafish adult brain,Sanger zebrafish sequencing,Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at 70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were re suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments.,Experimental Factor: ORGANISM PART:brain,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina Genome Analyzer II,1520Application ReadForward11Application ReadReverse77,ERP000447,Illumina Genome Analyzer II paired end sequencing; Sanger zebrafish sequencing,ENA FIRST PUBLIC:2011 02 03|ENA LAST UPDATE:2018 11 16,3212_6.srf,srf,1970995248.0,12967074.0,E MTAB 460:3212 6.srf,0:76 1:76,A:601407561;C:370471604;G:371296333;T:608476094;N:19343656,76,76,,,601407561,370471604,371296333,608476094,19343656,ERX009448,ERS017861,ERA015648,SC|Wellcome Trust Sanger Institute,SC|Wellcome Trust Sanger Institute,2,0.90042,0.89919,0.30246,0.30122,0.75534,0.75349,0.57375,0.56725,76,76,B,B,biological fallback assumption,illumina,early_illumina,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2011-02-03,Adult,Adult,Brain,Nervous System
8076,ERR2304209,ERX2355537,ERS2201745,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Aged mutant biorep3,SAMEA104590463,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590463|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Aged mutant biorep3|common name:zebrafish|sample name:Aged mutant biorep3,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 12,9 psen1K97Gfshet 24mth 13 03 2014 S3 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,9_psen1K97Gfshet_24mth_13_03_2014_S3_fem_R1.fastq.gz 9_psen1K97Gfshet_24mth_13_03_2014_S3_fem_R2.fastq.gz,fastq fastq,9318039088.0,38360343.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 12,0:121.17 1:121.74,A:2583644589;C:2091813317;G:2105322994;T:2536795040;N:463148,121,121,,,2583644589,2091813317,2105322994,2536795040,463148,ERX2355537,ERS2201745,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.93003,0.92836,0.26254,0.26157,0.68992,0.69593,0.48246,0.48292,134,134,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8077,ERR2304208,ERX2355536,ERS2201744,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Aged mutant biorep2,SAMEA104590462,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590462|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Aged mutant biorep2|common name:zebrafish|sample name:Aged mutant biorep2,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 11,8 psen1K97Gfshet 24mth 13 03 2014 S2 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,8_psen1K97Gfshet_24mth_13_03_2014_S2_fem_R1.fastq.gz 8_psen1K97Gfshet_24mth_13_03_2014_S2_fem_R2.fastq.gz,fastq fastq,8559244581.0,35608377.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 11,0:119.87 1:120.50,A:2397336730;C:1892236963;G:1910506310;T:2358778868;N:385710,119,120,,,2397336730,1892236963,1910506310,2358778868,385710,ERX2355536,ERS2201744,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.92422,0.92311,0.30514,0.30437,0.69578,0.7008,0.49054,0.48857,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8078,ERR2304207,ERX2355535,ERS2201743,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Aged mutant biorep1,SAMEA104590461,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590461|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Aged mutant biorep1|common name:zebrafish|sample name:Aged mutant biorep1,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 10,7 psen1K97Gfshet 24mth 13 03 2014 S1 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,7_psen1K97Gfshet_24mth_13_03_2014_S1_fem_R1.fastq.gz 7_psen1K97Gfshet_24mth_13_03_2014_S1_fem_R2.fastq.gz,fastq fastq,6521711648.0,27182062.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 10,0:119.65 1:120.27,A:1831722484;C:1434689482;G:1449266189;T:1805677755;N:355738,119,120,,,1831722484,1434689482,1449266189,1805677755,355738,ERX2355535,ERS2201743,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.92564,0.92498,0.29344,0.29212,0.69327,0.69964,0.48557,0.48942,86,86,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8079,ERR2304206,ERX2355534,ERS2201742,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Aged wild type biorep3,SAMEA104590460,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590460|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Aged wild type biorep3|common name:zebrafish|sample name:Aged wild type biorep3,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 9,3 non mutant K97Gfs 24mth 13 03 2014 S3 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,3_non_mutant_K97Gfs_24mth_13_03_2014_S3_fem_R1.fastq.gz 3_non_mutant_K97Gfs_24mth_13_03_2014_S3_fem_R2.fastq.gz,fastq fastq,6865452019.0,28646225.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 9,0:119.50 1:120.16,A:1903309108;C:1535570672;G:1550661363;T:1875578941;N:331935,119,120,,,1903309108,1535570672,1550661363,1875578941,331935,ERX2355534,ERS2201742,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.92997,0.92904,0.26949,0.26497,0.69485,0.70072,0.49378,0.50075,96,96,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8080,ERR2304205,ERX2355533,ERS2201741,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Aged wild type biorep2,SAMEA104590459,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590459|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Aged wild type biorep2|common name:zebrafish|sample name:Aged wild type biorep2,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 8,2 non mutant K97Gfs 24mth 13 03 2014 S2 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,2_non_mutant_K97Gfs_24mth_13_03_2014_S2_fem_R1.fastq.gz 2_non_mutant_K97Gfs_24mth_13_03_2014_S2_fem_R2.fastq.gz,fastq fastq,8418868343.0,34905186.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 8,0:120.29 1:120.91,A:2334515884;C:1885784857;G:1900432559;T:2297775998;N:359045,120,120,,,2334515884,1885784857,1900432559,2297775998,359045,ERX2355533,ERS2201741,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.93078,0.92967,0.25478,0.25365,0.69372,0.69938,0.4988,0.49709,132,132,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8081,ERR2304204,ERX2355532,ERS2201740,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Aged wild type biorep1,SAMEA104590458,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590458|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Aged wild type biorep1|common name:zebrafish|sample name:Aged wild type biorep1,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 7,1 non mutant K97Gfs 24mth 13 03 2014 S1 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,1_non_mutant_K97Gfs_24mth_13_03_2014_S1_fem_R1.fastq.gz 1_non_mutant_K97Gfs_24mth_13_03_2014_S1_fem_R2.fastq.gz,fastq fastq,6628468736.0,27477727.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 7,0:120.31 1:120.92,A:1839014115;C:1487750495;G:1497160978;T:1804205119;N:338029,120,120,,,1839014115,1487750495,1497160978,1804205119,338029,ERX2355532,ERS2201740,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.92916,0.92783,0.28453,0.28375,0.69798,0.70289,0.48132,0.48227,125,125,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8082,ERR2304203,ERX2355531,ERS2201739,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Young mutant biorep3,SAMEA104590457,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590457|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Young mutant biorep3|common name:zebrafish|sample name:Young mutant biorep3,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 6,12 psen1K97Gfshet 6mth 10 03 2016 S3 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,12_psen1K97Gfshet_6mth_10_03_2016_S3_fem_R1.fastq.gz 12_psen1K97Gfshet_6mth_10_03_2016_S3_fem_R2.fastq.gz,fastq fastq,11485397100.0,38284657.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 6,0:150 1:150,A:3206707597;C:2539306860;G:2721427816;T:3015264084;N:2690743,150,150,,,3206707597,2539306860,2721427816,3015264084,2690743,ERX2355531,ERS2201739,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.92853,0.92813,0.26757,0.26433,0.68487,0.68903,0.47708,0.47074,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8083,ERR2304202,ERX2355530,ERS2201738,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Young mutant biorep2,SAMEA104590456,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590456|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Young mutant biorep2|common name:zebrafish|sample name:Young mutant biorep2,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 5,11 psen1K97Gfshet 6mth 10 03 2016 S2 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,11_psen1K97Gfshet_6mth_10_03_2016_S2_fem_R1.fastq.gz 11_psen1K97Gfshet_6mth_10_03_2016_S2_fem_R2.fastq.gz,fastq fastq,13258122000.0,44193740.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 5,0:150 1:150,A:3781076311;C:2868334319;G:3063279426;T:3542309854;N:3122090,150,150,,,3781076311,2868334319,3063279426,3542309854,3122090,ERX2355530,ERS2201738,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.91552,0.91659,0.32337,0.32168,0.69546,0.698,0.4697,0.47295,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8084,ERR2304201,ERX2355529,ERS2201737,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Young mutant biorep1,SAMEA104590455,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590455|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Young mutant biorep1|common name:zebrafish|sample name:Young mutant biorep1,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 4,10 psen1K97Gfshet 6mth 10 03 2016 S1 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,10_psen1K97Gfshet_6mth_10_03_2016_S1_fem_R1.fastq.gz 10_psen1K97Gfshet_6mth_10_03_2016_S1_fem_R2.fastq.gz,fastq fastq,11724649800.0,39082166.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 4,0:150 1:150,A:3304100658;C:2560616667;G:2779636286;T:3077545106;N:2751083,150,150,,,3304100658,2560616667,2779636286,3077545106,2751083,ERX2355529,ERS2201737,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.92121,0.91893,0.28263,0.27928,0.69073,0.6953,0.47174,0.46157,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8085,ERR2304200,ERX2355528,ERS2201736,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Young wild type biorep3,SAMEA104590454,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590454|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Young wild type biorep3|common name:zebrafish|sample name:Young wild type biorep3,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 3,6 non mutant K97Gfs 6mth 10 03 2016 S3 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,6_non_mutant_K97Gfs_6mth_10_03_2016_S3_fem_R1.fastq.gz 6_non_mutant_K97Gfs_6mth_10_03_2016_S3_fem_R2.fastq.gz,fastq fastq,24923212200.0,83077374.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 3,0:150 1:150,A:7077378299;C:5398402396;G:5838059014;T:6604505033;N:4867458,150,150,,,7077378299,5398402396,5838059014,6604505033,4867458,ERX2355528,ERS2201736,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.91973,0.92144,0.29148,0.29015,0.69587,0.69994,0.46145,0.47047,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8086,ERR2304199,ERX2355527,ERS2201735,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Young wild type biorep2,SAMEA104590453,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590453|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Young wild type biorep2|common name:zebrafish|sample name:Young wild type biorep2,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 2,5 non mutant K97Gfs 6mth 10 03 2016 S2 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,5_non_mutant_K97Gfs_6mth_10_03_2016_S2_fem_R1.fastq.gz 5_non_mutant_K97Gfs_6mth_10_03_2016_S2_fem_R2.fastq.gz,fastq fastq,7840317153.0,39006553.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 2,0:101 1:100,A:2200490653;C:1718458832;G:1730278863;T:2188900320;N:2188485,101,100,,,2200490653,1718458832,1730278863,2188900320,2188485,ERX2355527,ERS2201735,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.91783,0.91983,0.32092,0.32105,0.67714,0.67691,0.47312,0.47481,101,100,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8087,ERR2304198,ERX2355526,ERS2201734,ERP106721,PRJEB24858,Zebrafish modelling familialAlzheimer's disease using heterozygous K97fs mutation in locus psen1,ena-STUDY-Adelaide Bioinformatics Hub-08-02-2018-04:47:34:743-1099,Other,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease Total RNA was extracted from the whole brains of mutant and wild type zebrafish when they were either 6 mpf young adult or 24 mpf infertile adult. Mutant zebrafish possess a heterozygous K97fs mutation at the endogenous zebrafish psen1 locus while wild type zebrafish do not.,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 02 08,,,Young wild type biorep1,SAMEA104590452,Adelaide Bioinformatics Hub,ENA first public:2018 05 11|ENA last update:2018 02 14|External Id:SAMEA104590452|INSDC center alias:Adelaide Bioinformatics Hub|INSDC center name:Adelaide Bioinformatics Hub|INSDC first public:2018 05 11T17:03:03Z|INSDC last update:2018 02 14T06:16:03Z|INSDC status:public|Submitter Id:Young wild type biorep1|common name:zebrafish|sample name:Young wild type biorep1,,,,,,,,,NextSeq 500 paired end sequencing,ena EXPERIMENT Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 1,4 non mutant K97Gfs 6mth 10 03 2016 S1 fem,RNA seq analysis of whole brains from zebrafish possessing a heterozygous K97fs mutation in psen1 to model familial Alzheimer's disease,Total RNA was extracted from whole brains using the mirVana miRNA isolation kit ThermoFisher using the manufacturer's protocol.,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,NextSeq 500,,ERP106721,NextSeq 500 paired end sequencing,ENA FIRST PUBLIC:2018 05 11|ENA LAST UPDATE:2018 11 16,4_non_mutant_K97Gfs_6mth_10_03_2016_S1_fem_R1.fastq.gz 4_non_mutant_K97Gfs_6mth_10_03_2016_S1_fem_R2.fastq.gz,fastq fastq,13910901600.0,46369672.0,ena RUN Adelaide Bioinformatics Hub 15 02 2018 04:44:34:534 1,0:150 1:150,A:3994738757;C:2957179645;G:3191379676;T:3764325893;N:3277629,150,150,,,3994738757,2957179645,3191379676,3764325893,3277629,ERX2355526,ERS2201734,ERA1210082,Adelaide Bioinformatics Hub|European Nucleotide Archive,Adelaide Bioinformatics Hub,2,0.91399,0.9166,0.3202,0.31818,0.6942,0.698,0.46902,0.47111,150,150,B,B,biological fallback assumption,illumina,nextseq,unknown,random_priming,unknown,bulk,unknown,unknown,,Australia,2018-02-08,Undetermined,Adult,Brain,Nervous System
8108,ERR2716246,ERX2730409,ERS2626924,ERP110098,PRJEB27957,Strand specific RNA sequencing of specific brain regions of the zebrafish,ena-STUDY-Biochemical Adaptation Laboratory-27-07-2018-11:07:45:884-1251,Other,Total RNA was isolated from three brain regions forebrain midbrain and hindbrain of wild type adult zebrafish Danio rerio. Strand specific RNA was sequenced post ribosomal depletion by Ribo Zero gold kit. Long non coding RNAs lncRNAs and mRNAs were detected and differential expression studied.,ENA FIRST PUBLIC:2018 09 26|ENA LAST UPDATE:2018 07 27,,Control forebrain RNA seq in zebrafish,Forebrain control,SAMEA4807015,Biochemical Adaptation Laboratory,ENA FIRST PUBLIC:2018 09 26T17:03:18Z|ENA LAST UPDATE:2018 07 27T11:15:51Z|External Id:SAMEA4807015|INSDC center name:Biochemical Adaptation Laboratory|INSDC first public:2018 09 26T17:03:18Z|INSDC last update:2018 07 27T11:15:51Z|INSDC status:public|Submitter Id:Fbc2|common name:zebrafish|dev stage:adult|sample name:Fbc2|scientific name:Danio rerio|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT Biochemical Adaptation Laboratory 27 07 2018 11:28:10:380 2,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP110098,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2018 09 26|ENA LAST UPDATE:2018 11 16,Fbc2_R1.fastq.gz Fbc2_R2.fastq.gz,fastq fastq,3163293134.0,15659867.0,ena RUN Biochemical Adaptation Laboratory 27 07 2018 11:28:10:380 2,0:101 1:101,A:902663616;C:674083265;G:685089226;T:901443399;N:13628,101,101,,,902663616,674083265,685089226,901443399,13628,ERX2730409,ERS2626924,ERA1552935,Biochemical Adaptation Laboratory|European Nucleotide Archive,Biochemical Adaptation Laboratory,2,0.83256,0.83349,0.43782,0.43591,0.71514,0.71644,0.49741,0.48294,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,ribozero,bulk,unknown,unknown,,India,2018-07-27,Adult,Adult,Brain,Nervous System
8109,ERR2716245,ERX2730408,ERS2626923,ERP110098,PRJEB27957,Strand specific RNA sequencing of specific brain regions of the zebrafish,ena-STUDY-Biochemical Adaptation Laboratory-27-07-2018-11:07:45:884-1251,Other,Total RNA was isolated from three brain regions forebrain midbrain and hindbrain of wild type adult zebrafish Danio rerio. Strand specific RNA was sequenced post ribosomal depletion by Ribo Zero gold kit. Long non coding RNAs lncRNAs and mRNAs were detected and differential expression studied.,ENA FIRST PUBLIC:2018 09 26|ENA LAST UPDATE:2018 07 27,,Control forebrain RNA seq in zebrafish,Forebrain control,SAMEA4807014,Biochemical Adaptation Laboratory,ENA FIRST PUBLIC:2018 09 26T17:03:18Z|ENA LAST UPDATE:2018 07 27T11:15:46Z|External Id:SAMEA4807014|INSDC center name:Biochemical Adaptation Laboratory|INSDC first public:2018 09 26T17:03:18Z|INSDC last update:2018 07 27T11:15:46Z|INSDC status:public|Submitter Id:Fbc1|common name:zebrafish|dev stage:adult|sample name:Fbc1|scientific name:Danio rerio|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT Biochemical Adaptation Laboratory 27 07 2018 11:28:10:379 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP110098,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2018 09 26|ENA LAST UPDATE:2018 11 16,Fbc1_R1.fastq.gz Fbc1_R2.fastq.gz,fastq fastq,3304372156.0,16358278.0,ena RUN Biochemical Adaptation Laboratory 27 07 2018 11:28:10:379 1,0:101 1:101,A:950810615;C:696498715;G:708600684;T:948447898;N:14244,101,101,,,950810615,696498715,708600684,948447898,14244,ERX2730408,ERS2626923,ERA1552935,Biochemical Adaptation Laboratory|European Nucleotide Archive,Biochemical Adaptation Laboratory,2,0.46343,0.46503,0.21205,0.21055,0.7792,0.77674,0.4708,0.47581,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,ribozero,bulk,unknown,unknown,,India,2018-07-27,Adult,Adult,Brain,Nervous System
9331,ERR2862354,ERX2868592,ERS2866329,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,TUC,SAMEA5055152,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055152|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:TUC|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:TUC|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:TUC p,TUC p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,AGCGATAG_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz AGCGATAG_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz,fastq fastq,12890102400.0,64450512.0,E MTAB 7349:AGCGATAG AGGCTATA 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3347844573;C:3078635366;G:3118813009;T:3343385073;N:1424379,100,100,,,3347844573,3078635366,3118813009,3343385073,1424379,ERX2868592,ERS2866329,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.89165,0.8896,0.26768,0.26518,0.75142,0.75459,0.60855,0.6105,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System
9332,ERR2862353,ERX2868591,ERS2866328,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,TUB,SAMEA5055151,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055151|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:TUB|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:TUB|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:TUB p,TUB p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,TCTCGCGC_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz TCTCGCGC_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz,fastq fastq,15345882200.0,76729411.0,E MTAB 7349:TCTCGCGC AGGCTATA 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:4257362303;C:3394893224;G:3451042837;T:4240872487;N:1711349,100,100,,,4257362303,3394893224,3451042837,4240872487,1711349,ERX2868591,ERS2866328,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.86888,0.86735,0.45025,0.44476,0.72036,0.72529,0.56048,0.56274,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System
9333,ERR2862352,ERX2868590,ERS2866327,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,TUA,SAMEA5055150,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055150|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:TUA|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:TUA|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:TUA p,TUA p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,TCCGCGAA_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz TCCGCGAA_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz,fastq fastq,11985547000.0,59927735.0,E MTAB 7349:TCCGCGAA AGGCTATA 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3239652711;C:2732996991;G:2737809176;T:3273773368;N:1314754,100,100,,,3239652711,2732996991,2737809176,3273773368,1314754,ERX2868590,ERS2866327,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.91524,0.91585,0.32418,0.32053,0.72616,0.72671,0.59362,0.42478,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System
9334,ERR2862351,ERX2868589,ERS2866326,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,HRASC,SAMEA5055149,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055149|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:HRASC|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:HRASC|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:HRASC p,HRASC p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,CGGCTATG_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz CGGCTATG_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz,fastq fastq,11297151800.0,56485759.0,E MTAB 7349:CGGCTATG GTCAGTAC 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3202944476;C:2429970659;G:2461948398;T:3201079699;N:1208568,100,100,,,3202944476,2429970659,2461948398,3201079699,1208568,ERX2868589,ERS2866326,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.89842,0.86269,0.32455,0.30708,0.74245,0.75191,0.7454,0.74185,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System
9335,ERR2862350,ERX2868588,ERS2866325,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,HRASB 1A,SAMEA5055148,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055148|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:HRASB 1A|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:HRASB 1A|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:HRASB 1A p,HRASB 1A p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,TCTCGCGC_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz TCTCGCGC_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz,fastq fastq,13035635000.0,65178175.0,E MTAB 7349:TCTCGCGC GTCAGTAC 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3518767071;C:2991842048;G:3005014068;T:3518541297;N:1470516,100,100,,,3518767071,2991842048,3005014068,3518541297,1470516,ERX2868588,ERS2866325,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.89434,0.89384,0.28234,0.28093,0.7274,0.72813,0.6198,0.62424,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System
9336,ERR2862349,ERX2868587,ERS2866324,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,2A,SAMEA5055147,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055147|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:2A|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:2A|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:2A p,2A p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,TCCGCGAA_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz TCCGCGAA_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz,fastq fastq,11671772800.0,58358864.0,E MTAB 7349:TCCGCGAA GTCAGTAC 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3164806186;C:2661342106;G:2677258036;T:3167072669;N:1293803,100,100,,,3164806186,2661342106,2677258036,3167072669,1293803,ERX2868587,ERS2866324,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.89879,0.89878,0.28162,0.28061,0.73212,0.73452,0.59632,0.60115,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System
9356,ERR2983452,ERX2986068,ERS2955656,ERP112513,PRJEB30097,RNA Seq of Danionine species,E-MTAB-7476,Transcriptome Analysis,Tissues specific transcriptomes of Danio rerio Danio albolineatus and Danio aesculapii Brain Liver and Gonads.,ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05,,Protocols: Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers.,R3wB,SAMEA5147911,"University of Hamburg, Institute of Zoology, Molecular Animal Physiology",ENA FIRST PUBLIC:2018 12 05T17:02:27Z|ENA LAST UPDATE:2018 12 05T10:01:48Z|External Id:SAMEA5147911|INSDC center name:University of Hamburg Institute of Zoology Molecular Animal Physiology|INSDC first public:2018 12 05T17:02:27Z|INSDC last update:2018 12 05T10:01:48Z|INSDC status:public|Submitter Id:E MTAB 7476:R3wB|age:3|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|individual:3|organism part:brain|sample name:E MTAB 7476:R3wB|scientific name:Danio rerio|sex:female|strain:tu,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danio9 species,E MTAB 7476:R3wB p,R3wB p,RNA Seq of Danionine species,Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers.,Experimental Factor: organism:Danio rerio|Experimental Factor: organism part:brain,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina HiSeq 4000,2000FApplication ReadForward11RApplication ReadReverse101,ERP112513,Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danionine species,ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05,FCHNW2WBBXX_L6_HKZEBodsEAABRAAPEI-205_1.fq.gz FCHNW2WBBXX_L6_HKZEBodsEAABRAAPEI-205_2.fq.gz,fastq fastq,3622698000.0,18113490.0,E MTAB 7476:FCHNW2WBBXX L6 HKZEBodsEAABRAAPEI 205 ,0:100 1:100,A:988454110;C:825179148;G:816134465;T:992141776;N:788501,100,100,,,988454110,825179148,816134465,992141776,788501,ERX2986068,ERS2955656,ERA1674470,"University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive","University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive",2,0.9287,0.92856,0.14723,0.14721,0.6928,0.69469,0.4956,0.49616,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2018-12-05,Adult,Adult,Brain,Nervous System
9360,ERR2983448,ERX2986064,ERS2955652,ERP112513,PRJEB30097,RNA Seq of Danionine species,E-MTAB-7476,Transcriptome Analysis,Tissues specific transcriptomes of Danio rerio Danio albolineatus and Danio aesculapii Brain Liver and Gonads.,ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05,,Protocols: Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers.,R1wB,SAMEA5147907,"University of Hamburg, Institute of Zoology, Molecular Animal Physiology",ENA FIRST PUBLIC:2018 12 05T17:02:27Z|ENA LAST UPDATE:2018 12 05T10:01:48Z|External Id:SAMEA5147907|INSDC center name:University of Hamburg Institute of Zoology Molecular Animal Physiology|INSDC first public:2018 12 05T17:02:27Z|INSDC last update:2018 12 05T10:01:48Z|INSDC status:public|Submitter Id:E MTAB 7476:R1wB|age:3|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|individual:1|organism part:brain|sample name:E MTAB 7476:R1wB|scientific name:Danio rerio|sex:female|strain:tu,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danio9 species,E MTAB 7476:R1wB p,R1wB p,RNA Seq of Danionine species,Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers.,Experimental Factor: organism:Danio rerio|Experimental Factor: organism part:brain,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina HiSeq 4000,2000FApplication ReadForward11RApplication ReadReverse101,ERP112513,Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danionine species,ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05,FCHNW2WBBXX_L6_HKZEBodsEAAARAAPEI-202_1.fq.gz FCHNW2WBBXX_L6_HKZEBodsEAAARAAPEI-202_2.fq.gz,fastq fastq,3345687800.0,16728439.0,E MTAB 7476:FCHNW2WBBXX L6 HKZEBodsEAAARAAPEI 202 ,0:100 1:100,A:879841532;C:789462135;G:789187239;T:886468300;N:728594,100,100,,,879841532,789462135,789187239,886468300,728594,ERX2986064,ERS2955652,ERA1674470,"University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive","University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive",2,0.96514,0.96503,0.0325,0.0326,0.78967,0.79204,0.25943,0.26683,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Germany,2018-12-05,Adult,Adult,Brain,Nervous System
9927,ERR4321734,ERX4268592,ERS4808149,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni R 3,SAMEA7047515,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047515|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni R 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni R 3|sampling site:right cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni R 3 p,Ctrl uni R 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_R_3_1.txt Ctrl_uni_R_3_2.txt,fastq fastq,7001062560.0,43756641.0,E MTAB 9321:Ctrl uni R 3 ,0:80 1:80,A:1691997382;C:1785031961;G:1794429141;T:1728559618;N:1044458,80,80,,,1691997382,1785031961,1794429141,1728559618,1044458,ERX4268592,ERS4808149,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.93339,0.93586,0.30761,0.30942,0.77031,0.77285,0.6535,0.63203,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9928,ERR4321733,ERX4268591,ERS4808148,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni R 2,SAMEA7047514,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047514|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni R 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni R 2|sampling site:right cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni R 2 p,Ctrl uni R 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_R_2_1.txt Ctrl_uni_R_2_2.txt,fastq fastq,6922683200.0,43266770.0,E MTAB 9321:Ctrl uni R 2 ,0:80 1:80,A:1632897692;C:1799983634;G:1813073970;T:1675697152;N:1030752,80,80,,,1632897692,1799983634,1813073970,1675697152,1030752,ERX4268591,ERS4808148,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.9343,0.93678,0.30732,0.30767,0.77226,0.77496,0.67322,0.638,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9929,ERR4321732,ERX4268590,ERS4808147,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni R 1,SAMEA7047513,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047513|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni R 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni R 1|sampling site:right cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni R 1 p,Ctrl uni R 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_R_1_1.txt Ctrl_uni_R_1_2.txt,fastq fastq,7277767680.0,45486048.0,E MTAB 9321:Ctrl uni R 1 ,0:80 1:80,A:1716271935;C:1890805720;G:1910764817;T:1758823538;N:1101670,80,80,,,1716271935,1890805720,1910764817,1758823538,1101670,ERX4268590,ERS4808147,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.93132,0.93607,0.29495,0.29694,0.77916,0.78023,0.66985,0.69546,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9930,ERR4321731,ERX4268589,ERS4808146,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni L 3,SAMEA7047512,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047512|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni L 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni L 3|sampling site:left cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni L 3 p,Ctrl uni L 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_L_3_1.txt Ctrl_uni_L_3_2.txt,fastq fastq,6575179520.0,41094872.0,E MTAB 9321:Ctrl uni L 3 ,0:80 1:80,A:1482672127;C:1776068618;G:1790187841;T:1525260758;N:990176,80,80,,,1482672127,1776068618,1790187841,1525260758,990176,ERX4268589,ERS4808146,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.94459,0.94749,0.2565,0.25972,0.77816,0.77881,0.67376,0.6469,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9931,ERR4321730,ERX4268588,ERS4808145,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni L 2,SAMEA7047511,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047511|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni L 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni L 2|sampling site:left cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni L 2 p,Ctrl uni L 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_L_2_1.txt Ctrl_uni_L_2_2.txt,fastq fastq,7275818080.0,45473863.0,E MTAB 9321:Ctrl uni L 2 ,0:80 1:80,A:1706173762;C:1899925486;G:1918514584;T:1750098284;N:1105964,80,80,,,1706173762,1899925486,1918514584,1750098284,1105964,ERX4268588,ERS4808145,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.93448,0.93897,0.29227,0.29368,0.77299,0.77587,0.67483,0.69924,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9932,ERR4321729,ERX4268587,ERS4808144,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Ctrl uni L 1,SAMEA7047510,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047510|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:Ctrl uni L 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:Ctrl uni L 1|sampling site:left cerebral hemisphere|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:Ctrl uni L 1 p,Ctrl uni L 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:n1,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,Ctrl_uni_L_1_1.txt Ctrl_uni_L_1_2.txt,fastq fastq,6259547520.0,39122172.0,E MTAB 9321:Ctrl uni L 1 ,0:80 1:80,A:1504594075;C:1603950703;G:1614410199;T:1535635583;N:956960,80,80,,,1504594075,1603950703,1614410199,1535635583,956960,ERX4268587,ERS4808144,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.92994,0.93276,0.31313,0.31395,0.77195,0.77372,0.66568,0.6882,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9933,ERR4321728,ERX4268586,ERS4808143,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl uni 3,SAMEA7047509,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047509|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl uni 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl uni 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl uni 3 p,3dpl uni 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_uni_3_1.txt 3dpl_uni_3_2.txt,fastq fastq,7967977760.0,49799861.0,E MTAB 9321:3dpl uni 3 ,0:80 1:80,A:2260216672;C:1639328793;G:1824529883;T:2241676126;N:2226286,80,80,,,2260216672,1639328793,1824529883,2241676126,2226286,ERX4268586,ERS4808143,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.90526,0.90173,0.44801,0.44169,0.73827,0.74854,0.51874,0.52672,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9934,ERR4321727,ERX4268585,ERS4808142,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl uni 2,SAMEA7047508,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047508|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl uni 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl uni 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl uni 2 p,3dpl uni 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_uni_2_1.txt 3dpl_uni_2_2.txt,fastq fastq,9215409600.0,57596310.0,E MTAB 9321:3dpl uni 2 ,0:80 1:80,A:2620502481;C:1914345031;G:2080364714;T:2597606056;N:2591318,80,80,,,2620502481,1914345031,2080364714,2597606056,2591318,ERX4268585,ERS4808142,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91412,0.91102,0.42824,0.4207,0.73091,0.73671,0.52094,0.52307,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9935,ERR4321726,ERX4268584,ERS4808141,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl uni 1,SAMEA7047507,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047507|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl uni 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl uni 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl uni 1 p,3dpl uni 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_uni_1_1.txt 3dpl_uni_1_2.txt,fastq fastq,9713477280.0,60709233.0,E MTAB 9321:3dpl uni 1 ,0:80 1:80,A:2666518127;C:1984727973;G:2436235221;T:2623269258;N:2726701,80,80,,,2666518127,1984727973,2436235221,2623269258,2726701,ERX4268584,ERS4808141,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91468,0.89921,0.41764,0.40764,0.73403,0.74608,0.52391,0.52748,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9936,ERR4321725,ERX4268583,ERS4808140,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl uni 3,SAMEA7047506,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047506|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl uni 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl uni 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl uni 3 p,20hpl uni 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_uni_3_1.txt 20hpl_uni_3_2.txt,fastq fastq,8675853280.0,54224083.0,E MTAB 9321:20hpl uni 3 ,0:80 1:80,A:2365631089;C:1890091876;G:2067595442;T:2325000100;N:27534773,80,80,,,2365631089,1890091876,2067595442,2325000100,27534773,ERX4268583,ERS4808140,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.90101,0.90675,0.42722,0.4249,0.74337,0.75294,0.51417,0.52085,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9937,ERR4321724,ERX4268582,ERS4808139,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl uni 2,SAMEA7047505,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047505|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl uni 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl uni 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl uni 2 p,20hpl uni 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_uni_2_1.txt 20hpl_uni_2_2.txt,fastq fastq,4735091040.0,29594319.0,E MTAB 9321:20hpl uni 2 ,0:80 1:80,A:1061979324;C:1276598224;G:1297035795;T:1098757554;N:720143,80,80,,,1061979324,1276598224,1297035795,1098757554,720143,ERX4268582,ERS4808139,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.9384,0.94067,0.25644,0.25853,0.7877,0.78843,0.69474,0.72221,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9938,ERR4321723,ERX4268581,ERS4808138,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl uni 1,SAMEA7047504,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047504|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl uni 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl uni 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl uni 1 p,20hpl uni 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions DMSO was added to fish water Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:n1|Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_uni_1_1.txt 20hpl_uni_1_2.txt,fastq fastq,10122915040.0,63268219.0,E MTAB 9321:20hpl uni 1 ,0:80 1:80,A:2917523358;C:2094720604;G:2205586268;T:2872641443;N:32443367,80,80,,,2917523358,2094720604,2205586268,2872641443,32443367,ERX4268581,ERS4808138,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.90867,0.91132,0.46283,0.46241,0.74858,0.75357,0.53145,0.53522,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9939,ERR4321722,ERX4268580,ERS4808137,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in IWR 3,SAMEA7047503,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047503|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in IWR 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in IWR 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in IWR 3 p,3dpl in IWR 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_IWR_3_1.txt 3dpl_in_IWR_3_2.txt,fastq fastq,9476807840.0,59230049.0,E MTAB 9321:3dpl in IWR 3 ,0:80 1:80,A:2639849526;C:1994343199;G:2238787041;T:2601193111;N:2634963,80,80,,,2639849526,1994343199,2238787041,2601193111,2634963,ERX4268580,ERS4808137,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91666,0.90016,0.41957,0.41098,0.72679,0.73596,0.52875,0.53529,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9940,ERR4321721,ERX4268579,ERS4808136,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in IWR 2,SAMEA7047502,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047502|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in IWR 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in IWR 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in IWR 2 p,3dpl in IWR 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_IWR_2_1.txt 3dpl_in_IWR_2_2.txt,fastq fastq,7305898240.0,45661864.0,E MTAB 9321:3dpl in IWR 2 ,0:80 1:80,A:2035812198;C:1535985603;G:1714961805;T:2017139933;N:1998701,80,80,,,2035812198,1535985603,1714961805,2017139933,1998701,ERX4268579,ERS4808136,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91721,0.90787,0.40247,0.39455,0.72916,0.74097,0.52622,0.52732,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9941,ERR4321720,ERX4268578,ERS4808135,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,3dpl in IWR 1,SAMEA7047501,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047501|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:3dpl in IWR 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:3dpl in IWR 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:3dpl in IWR 1 p,3dpl in IWR 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:3,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,3dpl_in_IWR_1_1.txt 3dpl_in_IWR_1_2.txt,fastq fastq,5893946400.0,36837165.0,E MTAB 9321:3dpl in IWR 1 ,0:80 1:80,A:1170533263;C:1737932152;G:1758304403;T:1226290662;N:885920,80,80,,,1170533263,1737932152,1758304403,1226290662,885920,ERX4268578,ERS4808135,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.95436,0.95726,0.17474,0.17743,0.77031,0.7726,0.72793,0.73265,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9942,ERR4321719,ERX4268577,ERS4808134,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in IWR 3,SAMEA7047500,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047500|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in IWR 3|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in IWR 3|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in IWR 3 p,20hpl in IWR 3 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_IWR_3_1.txt 20hpl_in_IWR_3_2.txt,fastq fastq,6198105920.0,38738162.0,E MTAB 9321:20hpl in IWR 3 ,0:80 1:80,A:1441128066;C:1626141975;G:1647720111;T:1482170340;N:945428,80,80,,,1441128066,1626141975,1647720111,1482170340,945428,ERX4268577,ERS4808134,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.94334,0.94604,0.2532,0.25458,0.75941,0.76086,0.66584,0.67193,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9943,ERR4321718,ERX4268576,ERS4808133,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in IWR 2,SAMEA7047499,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047499|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in IWR 2|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in IWR 2|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in IWR 2 p,20hpl in IWR 2 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_IWR_2_1.txt 20hpl_in_IWR_2_2.txt,fastq fastq,8244868480.0,51530428.0,E MTAB 9321:20hpl in IWR 2 ,0:80 1:80,A:2326026663;C:1706321438;G:1913900194;T:2272224793;N:26395392,80,80,,,2326026663,1706321438,1913900194,2272224793,26395392,ERX4268576,ERS4808133,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91275,0.90955,0.44535,0.44255,0.74456,0.75298,0.53931,0.53823,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System
9944,ERR4321717,ERX4268575,ERS4808132,ERP122765,PRJEB39269,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß catenin signaling at the early wound healing stage,E-MTAB-9321,Transcriptome Analysis,Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing RNA Seq is a high throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA Seq technologies and their extensive data analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing 20 hour post lesion and early proliferative 3 day post lesion stages of regeneration. Moreover we uncover Wnt/ß catenin signaling as a key pathway that is activated at the early wound healing stage.,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 06,,Protocols: Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,20hpl in IWR 1,SAMEA7047498,Izmir Biomedicine and Genome Center,ENA FIRST PUBLIC:2020 10 05T04:05:55Z|ENA LAST UPDATE:2020 07 06T18:12:29Z|External Id:SAMEA7047498|INSDC center name:Izmir Biomedicine and Genome Center|INSDC first public:2020 10 05T04:05:55Z|INSDC last update:2020 07 06T18:12:29Z|INSDC status:public|Submitter Id:E MTAB 9321:20hpl in IWR 1|age:6 to 10|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:6xTCF/Lef miniP:2dGFP|organism part:telencephalon|sample name:E MTAB 9321:20hpl in IWR 1|scientific name:Danio rerio|sex:male,,,,,,,,,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,E MTAB 9321:20hpl in IWR 1 p,20hpl in IWR 1 p,Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4 1 3 3a 4 7 7a Hexahydro 1 3 dioxo 4 7 methano 2H isoindol 2 yl N 8 quinolinyl Benzamide IWR 1 was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later and Total RNA isolation was performed. RNA seq libraries were built using the TruSeq RNA Library Preparation v2 Kit Illumina,Experimental Factor: injury:stab lesion|Experimental Factor: compound:IWR 1|Experimental Factor: dose:20|Experimental Factor: time:20,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,NextSeq 500,,ERP122765,NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β catenin signaling at the early wound healing stage,ENA FIRST PUBLIC:2020 10 05|ENA LAST UPDATE:2020 07 07,20hpl_in_IWR_1_1.txt 20hpl_in_IWR_1_2.txt,fastq fastq,8946910240.0,55918189.0,E MTAB 9321:20hpl in IWR 1 ,0:80 1:80,A:2523280880;C:1876368998;G:2029673577;T:2489111763;N:28475022,80,80,,,2523280880,1876368998,2029673577,2489111763,28475022,ERX4268575,ERS4808132,ERA2763798,Izmir Biomedicine and Genome Center|European Nucleotide Archive,Izmir Biomedicine and Genome Center|European Nucleotide Archive,2,0.91067,0.90286,0.45601,0.44987,0.7335,0.74119,0.51443,0.51662,80,80,B,B,biological fallback assumption,illumina,nextseq,full_length,random_priming,trueseq,bulk,unknown,unknown,,Turkey,2020-07-06,Adult,Adult,Brain,Nervous System