rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 64331,SRR14551653,SRX10895137,SRS8987960,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,neuronal 72hpf Sample3,GSM5315776,,tissue:Zebrafish embryo trunk|strain:AB* Tgnbt:dsred|age:72 hpf,neuronal 72hpf Sample3,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Tgnbt:dsred|age:72 hpf,GSM5315776,GSM5315776: neuronal 72hpf Sample3; Danio rerio; RNA Seq,GSM5315776,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315776,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,neuronal72_Sample3_R1_001.fastq.gz neuronal72_Sample3_R2_001.fastq.gz,fastq fastq,5811578187.0,19461304.0,GSM5315776 r1,0:149.33 1:149.29,A:1529597778;C:1363449364;G:1361318435;T:1556834579;N:378031,149,149,,,1529597778,1363449364,1361318435,1556834579,378031,SRX10895137,SRS8987960,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.86088,0.86196,0.09968,0.09909,0.76952,0.77553,0.47402,0.46267,149,149,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure 64332,SRR14551652,SRX10895136,SRS8987961,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,neuronal 72hpf Sample2,GSM5315775,,tissue:Zebrafish embryo trunk|strain:AB* Tgnbt:dsred|age:72 hpf,neuronal 72hpf Sample2,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Tgnbt:dsred|age:72 hpf,GSM5315775,GSM5315775: neuronal 72hpf Sample2; Danio rerio; RNA Seq,GSM5315775,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315775,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,neuronal72_Sample2_R1_001.fastq.gz neuronal72_Sample2_R2_001.fastq.gz,fastq fastq,5330377491.0,17862084.0,GSM5315775 r1,0:149.23 1:149.19,A:1408165778;C:1247044167;G:1242879903;T:1431943960;N:343683,149,149,,,1408165778,1247044167,1242879903,1431943960,343683,SRX10895136,SRS8987961,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.82586,0.83096,0.1393,0.14169,0.7907,0.79555,0.46473,0.48234,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure 64333,SRR14551651,SRX10895135,SRS8987956,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,neuronal 72hpf Sample1,GSM5315774,,tissue:Zebrafish embryo trunk|strain:AB* Tgnbt:dsred|age:72 hpf,neuronal 72hpf Sample1,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Tgnbt:dsred|age:72 hpf,GSM5315774,GSM5315774: neuronal 72hpf Sample1; Danio rerio; RNA Seq,GSM5315774,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315774,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,neuronal72_Sample1_R1_001.fastq.gz neuronal72_Sample1_R2_001.fastq.gz,fastq fastq,7122524050.0,23888399.0,GSM5315774 r1,0:149.11 1:149.05,A:1867527557;C:1687169064;G:1683673406;T:1883615223;N:538800,149,149,,,1867527557,1687169064,1683673406,1883615223,538800,SRX10895135,SRS8987956,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.88793,0.88809,0.12727,0.12826,0.7486,0.75635,0.42346,0.48977,149,149,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure 64334,SRR14551650,SRX10895134,SRS8987957,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,OPC 72hpf Sample3,GSM5315773,,tissue:Zebrafish embryo trunk|strain:AB* Tgolig2:dsred; Tgsox10:megfp|age:72 hpf,OPC 72hpf Sample3,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Tgolig2:dsred;Tgsox10:megfp|age:72 hpf,GSM5315773,GSM5315773: OPC 72hpf Sample3; Danio rerio; RNA Seq,GSM5315773,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315773,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,OPC72_Sample3_R1_001.fastq.gz OPC72_Sample3_R2_001.fastq.gz,fastq fastq,6630077092.0,22212439.0,GSM5315773 r1,0:149.27 1:149.22,A:1681054163;C:1589572166;G:1588126414;T:1770833666;N:490683,149,149,,,1681054163,1589572166,1588126414,1770833666,490683,SRX10895134,SRS8987957,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.69031,0.6945,0.1106,0.1101,0.85123,0.85389,0.45515,0.45517,149,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure 64335,SRR14551649,SRX10895133,SRS8987958,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,OPC 72hpf Sample2,GSM5315772,,tissue:Zebrafish embryo trunk|strain:AB* Tgolig2:dsred; Tgsox10:megfp|age:72 hpf,OPC 72hpf Sample2,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Tgolig2:dsred;Tgsox10:megfp|age:72 hpf,GSM5315772,GSM5315772: OPC 72hpf Sample2; Danio rerio; RNA Seq,GSM5315772,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315772,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,OPC72_Sample2_R1_001.fastq.gz OPC72_Sample2_R2_001.fastq.gz,fastq fastq,7703607275.0,25797864.0,GSM5315772 r1,0:149.33 1:149.29,A:2052466926;C:1756697559;G:1751055673;T:2142874669;N:512448,149,149,,,2052466926,1756697559,1751055673,2142874669,512448,SRX10895133,SRS8987958,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.78396,0.7872,0.15128,0.15083,0.80176,0.8043,0.47044,0.47071,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure 64336,SRR14551648,SRX10895132,SRS8987959,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,OPC 72hpf Sample1,GSM5315771,,tissue:Zebrafish embryo trunk|strain:AB* Tgolig2:dsred; Tgsox10:megfp|age:72 hpf,OPC 72hpf Sample1,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Tgolig2:dsred;Tgsox10:megfp|age:72 hpf,GSM5315771,GSM5315771: OPC 72hpf Sample1; Danio rerio; RNA Seq,GSM5315771,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315771,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,OPC72_Sample1_R1_001.fastq.gz OPC72_Sample1_R2_001.fastq.gz,fastq fastq,6819055600.0,22845167.0,GSM5315771 r1,0:149.27 1:149.22,A:1844296500;C:1523361309;G:1519052310;T:1931907615;N:437866,149,149,,,1844296500,1523361309,1519052310,1931907615,437866,SRX10895132,SRS8987959,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.83297,0.83417,0.1328,0.13254,0.78821,0.79318,0.46692,0.4635,150,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure 64337,SRR14551647,SRX10895131,SRS8987955,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,SC 72hpf Sample3,GSM5315770,,tissue:Zebrafish embryo trunk|strain:AB* Gtfoxd3:mCherry; Tgsox10:mEGFP|age:72 hpf,SC 72hpf Sample3,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Gtfoxd3:mCherry;Tgsox10:mEGFP|age:72 hpf,GSM5315770,GSM5315770: SC 72hpf Sample3; Danio rerio; RNA Seq,GSM5315770,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315770,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,SC72_Sample3_R1_001.fastq.gz SC72_Sample3_R2_001.fastq.gz,fastq fastq,5290486005.0,17724499.0,GSM5315770 r1,0:149.26 1:149.22,A:1416646219;C:1205875601;G:1205458968;T:1462077308;N:427909,149,149,,,1416646219,1205875601,1205458968,1462077308,427909,SRX10895131,SRS8987955,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.80356,0.80484,0.13757,0.13551,0.79017,0.79569,0.464,0.46891,149,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure 64338,SRR14551646,SRX10895130,SRS8987954,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,SC 72hpf Sample2,GSM5315769,,tissue:Zebrafish embryo trunk|strain:AB* Gtfoxd3:mCherry; Tgsox10:mEGFP|age:72 hpf,SC 72hpf Sample2,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Gtfoxd3:mCherry;Tgsox10:mEGFP|age:72 hpf,GSM5315769,GSM5315769: SC 72hpf Sample2; Danio rerio; RNA Seq,GSM5315769,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315769,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,SC72_Sample2_R1_001.fastq.gz SC72_Sample2_R2_001.fastq.gz,fastq fastq,5300704321.0,17740513.0,GSM5315769 r1,0:149.42 1:149.37,A:1270635814;C:1361971808;G:1357145534;T:1310555763;N:395402,149,149,,,1270635814,1361971808,1357145534,1310555763,395402,SRX10895130,SRS8987954,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.52849,0.53398,0.10216,0.10317,0.887,0.88956,0.49141,0.49821,150,148,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure 64339,SRR14551645,SRX10895129,SRS8987953,SRP319894,PRJNA730034,Zebrafish Danio rerio Schwann cell oligodendrocyte lineage and neuron RNA sequencing,GSE174486,Transcriptome Analysis,RNA sequencing of cells collected from 72 hpf embryos: Schwann cells Gtfoxd3:mCherry;Tgsox10:mEGFP oligodendrocyte lineage cells Tgolig2:dsred;Tgsox10:megfp and neurons Tgnbt:dsred Overall design: Cells were collected from 72 hpf embryos as follows and sent for sequencing. For Schwann cells foxd3+/sox10+ double positive cells were collected from trunks of Gtfoxd3:mCherry;Tgsox10:mEGFP embryos. For oligodendrocyte lineage cells olig2+/sox10+ double positive cells were collected from the trunks of Tgolig2:dsred;Tgsox10:megfp embryos. For neurons nbt+ cells were collected from the trunks of Tgnbt:dsred embryos. 3 replicates for each condition.,,pubmed:33975920,,SC 72hpf Sample1,GSM5315768,,tissue:Zebrafish embryo trunk|strain:AB* Gtfoxd3:mCherry; Tgsox10:mEGFP|age:72 hpf,SC 72hpf Sample1,For data quality control FASTQC was used to check the raw fastq data quality and Trimmomatic was used to remove adaptors and to trim quality bases. post adapter clipping we removed leading and trailing ambiguous or low quality bases below Phred quality scores of 3. Trimmomatic works with a user defined window spanning the read from 5′ to 3′ and removes bases only at the 3′ end; we set up a window length of 4 and a quality threshold Q of 20. When the average quality drops below 20 the 3′ end is clipped. The reads were then mapped to the latest UCSC danRer10 transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15. Genome build: danRer10 Supplementary files format and content: normalized FPKM counts with annotations,Zebrafish embryo trunk,72 hpf embryos were chilled in egg water on ice and the anterior halves of the embryos were removed using a scalpel. Trunk pieces were placed in calcium free Ringer’s solution with 2.5mM EDTA and rocked for 15 minutes at 4 °C. The trunk pieces were washed three times with chilled Dulbecco’s PBS D PBS and then transferred to microcentrifuge tubes along with 100ul D PBS. A pellet pestle was used to break up the trunk pieces and then 30ul Liberase TM was added. The samples were incubated for 15 minutes at 28.5 °C 1ml 1x Trypsin with 5% EDTA solution was added and the samples were incubated for 15 more minutes at 28.5 °C. The samples were pipetted into 5ml D PBS with 1% BSA and then passed through a 40 μm cell strainer and a syringe plunger was used to gently mash the samples into a petri dish. The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D PBS+BSA. Three samples of cells 1750 ± 750 at each stage were used for RNA sequencing.,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,Gtfoxd3:mCherry;Tgsox10:mEGFP for Schwann cells Tgolig2:dsred;Tgsox10:megfp for OPC and Tgnbt:dsred for neurons zebrafish embryos were raised in egg water at 28.5C to 72 hpf,strain:AB* Gtfoxd3:mCherry;Tgsox10:mEGFP|age:72 hpf,GSM5315768,GSM5315768: SC 72hpf Sample1; Danio rerio; RNA Seq,GSM5315768,,1,Qiagen rneasy micro Smartseq v4 Ultra Low Input RNA Kit for Sequencing,GEO Accession:GSM5315768,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,,SRP319894,,loader:latf load,SC72_Sample1_R1_001.fastq.gz SC72_Sample1_R2_001.fastq.gz,fastq fastq,5239512991.0,17546095.0,GSM5315768 r1,0:149.33 1:149.28,A:1298269567;C:1306015004;G:1299170862;T:1335241575;N:815983,149,149,,,1298269567,1306015004,1299170862,1335241575,815983,SRX10895129,SRS8987953,SRA1232603,GEO,"Kucenas Lab, Biology, University of Virginia",2,0.61524,0.62125,0.10182,0.10151,0.87393,0.87825,0.47941,0.47885,149,150,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,United States,2021-05-14,Larval,Larval,Trunk,Surface Structure