rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 54735,SRR10151942,SRX6877449,SRS5413231,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s383,GSM4087504,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s383,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087504,GSM4087504: lckgfp s383; Danio rerio; RNA Seq,GSM4087504,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087504,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s383_R1.fastq.gz lckgfp_s383_R2.fastq.gz,fastq fastq,234832600.0,1174163.0,GSM4087504 r1,0:100 1:100,A:73305400;C:48312945;G:46608604;T:66604424;N:1227,100,100,,,73305400,48312945,46608604,66604424,1227,SRX6877449,SRS5413231,SRA965286,GEO,Stanford University,2,0.75941,0.76379,0.1177,0.1196,0.97948,0.9793,0.67812,0.67194,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54736,SRR10151941,SRX6877448,SRS5413230,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s382,GSM4087503,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s382,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087503,GSM4087503: lckgfp s382; Danio rerio; RNA Seq,GSM4087503,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087503,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s382_R1.fastq.gz lckgfp_s382_R2.fastq.gz,fastq fastq,231321400.0,1156607.0,GSM4087503 r1,0:100 1:100,A:74109121;C:45769344;G:44284415;T:67157279;N:1241,100,100,,,74109121,45769344,44284415,67157279,1241,SRX6877448,SRS5413230,SRA965286,GEO,Stanford University,2,0.71327,0.73209,0.11938,0.12294,0.98113,0.98121,0.67445,0.67189,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54737,SRR10151940,SRX6877447,SRS5413229,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s381,GSM4087502,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s381,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087502,GSM4087502: lckgfp s381; Danio rerio; RNA Seq,GSM4087502,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087502,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s381_R1.fastq.gz lckgfp_s381_R2.fastq.gz,fastq fastq,245645600.0,1228228.0,GSM4087502 r1,0:100 1:100,A:73876655;C:46349996;G:46669257;T:78748704;N:988,100,100,,,73876655,46349996,46669257,78748704,988,SRX6877447,SRS5413229,SRA965286,GEO,Stanford University,2,0.84889,0.85255,0.24599,0.25118,0.9584,0.9586,0.60851,0.60597,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54738,SRR10151939,SRX6877446,SRS5413228,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s380,GSM4087501,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s380,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087501,GSM4087501: lckgfp s380; Danio rerio; RNA Seq,GSM4087501,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087501,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s380_R1.fastq.gz lckgfp_s380_R2.fastq.gz,fastq fastq,392055200.0,1960276.0,GSM4087501 r1,0:100 1:100,A:118333309;C:79802020;G:77119877;T:116798071;N:1923,100,100,,,118333309,79802020,77119877,116798071,1923,SRX6877446,SRS5413228,SRA965286,GEO,Stanford University,2,0.78714,0.7986,0.11307,0.1166,0.94466,0.94505,0.72339,0.72034,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54739,SRR10151938,SRX6877445,SRS5413227,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s379,GSM4087500,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s379,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087500,GSM4087500: lckgfp s379; Danio rerio; RNA Seq,GSM4087500,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087500,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s379_R1.fastq.gz lckgfp_s379_R2.fastq.gz,fastq fastq,1126607400.0,5633037.0,GSM4087500 r1,0:100 1:100,A:316942811;C:247242301;G:247434168;T:314982567;N:5553,100,100,,,316942811,247242301,247434168,314982567,5553,SRX6877445,SRS5413227,SRA965286,GEO,Stanford University,2,0.90628,0.90945,0.2181,0.22037,0.97964,0.97958,0.47443,0.49146,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54740,SRR10151937,SRX6877444,SRS5413226,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s378,GSM4087499,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s378,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087499,GSM4087499: lckgfp s378; Danio rerio; RNA Seq,GSM4087499,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087499,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s378_R1.fastq.gz lckgfp_s378_R2.fastq.gz,fastq fastq,337111400.0,1685557.0,GSM4087499 r1,0:100 1:100,A:98638322;C:70570254;G:69961020;T:97940105;N:1699,100,100,,,98638322,70570254,69961020,97940105,1699,SRX6877444,SRS5413226,SRA965286,GEO,Stanford University,2,0.87386,0.87621,0.19517,0.19574,0.97443,0.97463,0.60077,0.60132,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54741,SRR10151936,SRX6877443,SRS5413225,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s377,GSM4087498,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s377,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087498,GSM4087498: lckgfp s377; Danio rerio; RNA Seq,GSM4087498,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087498,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s377_R1.fastq.gz lckgfp_s377_R2.fastq.gz,fastq fastq,213595800.0,1067979.0,GSM4087498 r1,0:100 1:100,A:65929082;C:44373444;G:41959720;T:61332589;N:965,100,100,,,65929082,44373444,41959720,61332589,965,SRX6877443,SRS5413225,SRA965286,GEO,Stanford University,2,0.63317,0.65413,0.48755,0.50508,0.96426,0.96317,0.67368,0.66493,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54742,SRR10151935,SRX6877442,SRS5413224,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s376,GSM4087497,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s376,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087497,GSM4087497: lckgfp s376; Danio rerio; RNA Seq,GSM4087497,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087497,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s376_R1.fastq.gz lckgfp_s376_R2.fastq.gz,fastq fastq,88560200.0,442801.0,GSM4087497 r1,0:100 1:100,A:28243730;C:14191830;G:14708934;T:31415270;N:436,100,100,,,28243730,14191830,14708934,31415270,436,SRX6877442,SRS5413224,SRA965286,GEO,Stanford University,2,0.71521,0.72283,0.5654,0.57182,0.92989,0.9317,0.531,0.52094,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54743,SRR10151934,SRX6877441,SRS5413223,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s375,GSM4087496,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s375,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087496,GSM4087496: lckgfp s375; Danio rerio; RNA Seq,GSM4087496,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087496,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s375_R1.fastq.gz lckgfp_s375_R2.fastq.gz,fastq fastq,64991200.0,324956.0,GSM4087496 r1,0:100 1:100,A:22513291;C:12471508;G:11416691;T:18589412;N:298,100,100,,,22513291,12471508,11416691,18589412,298,SRX6877441,SRS5413223,SRA965286,GEO,Stanford University,2,0.5963,0.62806,0.27588,0.2908,0.9754,0.975,0.44766,0.44857,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54744,SRR10151933,SRX6877440,SRS5413222,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s374,GSM4087495,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s374,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087495,GSM4087495: lckgfp s374; Danio rerio; RNA Seq,GSM4087495,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087495,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s374_R1.fastq.gz lckgfp_s374_R2.fastq.gz,fastq fastq,176144200.0,880721.0,GSM4087495 r1,0:100 1:100,A:53180935;C:37108779;G:35845658;T:50007992;N:836,100,100,,,53180935,37108779,35845658,50007992,836,SRX6877440,SRS5413222,SRA965286,GEO,Stanford University,2,0.76708,0.77852,0.13854,0.14107,0.98238,0.98283,0.66594,0.67246,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54745,SRR10151932,SRX6877439,SRS5413221,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s373,GSM4087494,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s373,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087494,GSM4087494: lckgfp s373; Danio rerio; RNA Seq,GSM4087494,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087494,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s373_R1.fastq.gz lckgfp_s373_R2.fastq.gz,fastq fastq,139717800.0,698589.0,GSM4087494 r1,0:100 1:100,A:42519883;C:25961082;G:25907850;T:45328266;N:719,100,100,,,42519883,25961082,25907850,45328266,719,SRX6877439,SRS5413221,SRA965286,GEO,Stanford University,2,0.75679,0.76819,0.28064,0.29002,0.97305,0.97273,0.56393,0.53945,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54746,SRR10151931,SRX6877438,SRS5413220,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s372,GSM4087493,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s372,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087493,GSM4087493: lckgfp s372; Danio rerio; RNA Seq,GSM4087493,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087493,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s372_R1.fastq.gz lckgfp_s372_R2.fastq.gz,fastq fastq,446412600.0,2232063.0,GSM4087493 r1,0:100 1:100,A:128821576;C:93782508;G:93694322;T:130111961;N:2233,100,100,,,128821576,93782508,93694322,130111961,2233,SRX6877438,SRS5413220,SRA965286,GEO,Stanford University,2,0.89125,0.89493,0.23701,0.24011,0.9697,0.97005,0.53362,0.53735,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54747,SRR10151930,SRX6877437,SRS5413219,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s371,GSM4087492,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s371,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087492,GSM4087492: lckgfp s371; Danio rerio; RNA Seq,GSM4087492,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087492,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s371_R1.fastq.gz lckgfp_s371_R2.fastq.gz,fastq fastq,339631800.0,1698159.0,GSM4087492 r1,0:100 1:100,A:98594117;C:68506566;G:69103975;T:103425575;N:1567,100,100,,,98594117,68506566,69103975,103425575,1567,SRX6877437,SRS5413219,SRA965286,GEO,Stanford University,2,0.84886,0.85388,0.19246,0.19436,0.95014,0.95012,0.54321,0.54848,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54748,SRR10151929,SRX6877436,SRS5413218,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s370,GSM4087491,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s370,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087491,GSM4087491: lckgfp s370; Danio rerio; RNA Seq,GSM4087491,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087491,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s370_R1.fastq.gz lckgfp_s370_R2.fastq.gz,fastq fastq,87104400.0,435522.0,GSM4087491 r1,0:100 1:100,A:26443732;C:16935041;G:16826001;T:26899213;N:413,100,100,,,26443732,16935041,16826001,26899213,413,SRX6877436,SRS5413218,SRA965286,GEO,Stanford University,2,0.82125,0.82733,0.31193,0.31596,0.97453,0.97433,0.60892,0.61117,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54749,SRR10151928,SRX6877435,SRS5413217,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s369,GSM4087490,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s369,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087490,GSM4087490: lckgfp s369; Danio rerio; RNA Seq,GSM4087490,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087490,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s369_R1.fastq.gz lckgfp_s369_R2.fastq.gz,fastq fastq,581275000.0,2906375.0,GSM4087490 r1,0:100 1:100,A:164842289;C:120994794;G:122636896;T:172798268;N:2753,100,100,,,164842289,120994794,122636896,172798268,2753,SRX6877435,SRS5413217,SRA965286,GEO,Stanford University,2,0.88299,0.88484,0.19686,0.19809,0.95436,0.95408,0.56675,0.57288,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54750,SRR10151927,SRX6877434,SRS5413216,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s368,GSM4087489,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s368,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087489,GSM4087489: lckgfp s368; Danio rerio; RNA Seq,GSM4087489,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087489,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s368_R1.fastq.gz lckgfp_s368_R2.fastq.gz,fastq fastq,312312800.0,1561564.0,GSM4087489 r1,0:100 1:100,A:92531881;C:61673383;G:61568700;T:96537155;N:1681,100,100,,,92531881,61673383,61568700,96537155,1681,SRX6877434,SRS5413216,SRA965286,GEO,Stanford University,2,0.82667,0.83385,0.29498,0.29709,0.97344,0.97366,0.54305,0.54647,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54751,SRR10151926,SRX6877433,SRS5413215,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s367,GSM4087488,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s367,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087488,GSM4087488: lckgfp s367; Danio rerio; RNA Seq,GSM4087488,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087488,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s367_R1.fastq.gz lckgfp_s367_R2.fastq.gz,fastq fastq,46265000.0,231325.0,GSM4087488 r1,0:100 1:100,A:13465697;C:8956151;G:9229542;T:14613421;N:189,100,100,,,13465697,8956151,9229542,14613421,189,SRX6877433,SRS5413215,SRA965286,GEO,Stanford University,2,0.84333,0.8365,0.28527,0.2816,0.97561,0.97469,0.52056,0.52116,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54752,SRR10151925,SRX6877432,SRS5413214,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s366,GSM4087487,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s366,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087487,GSM4087487: lckgfp s366; Danio rerio; RNA Seq,GSM4087487,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087487,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s366_R1.fastq.gz lckgfp_s366_R2.fastq.gz,fastq fastq,286132600.0,1430663.0,GSM4087487 r1,0:100 1:100,A:86110388;C:58112895;G:57034401;T:84873652;N:1264,100,100,,,86110388,58112895,57034401,84873652,1264,SRX6877432,SRS5413214,SRA965286,GEO,Stanford University,2,0.7188,0.73304,0.23183,0.23804,0.97195,0.9724,0.60395,0.60083,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54753,SRR10151924,SRX6877431,SRS5413213,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s365,GSM4087486,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s365,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087486,GSM4087486: lckgfp s365; Danio rerio; RNA Seq,GSM4087486,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087486,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s365_R1.fastq.gz lckgfp_s365_R2.fastq.gz,fastq fastq,129864000.0,649320.0,GSM4087486 r1,0:100 1:100,A:42306739;C:23190789;G:22929572;T:41436300;N:600,100,100,,,42306739,23190789,22929572,41436300,600,SRX6877431,SRS5413213,SRA965286,GEO,Stanford University,2,0.67875,0.694,0.29442,0.30278,0.98206,0.98212,0.60421,0.60135,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54754,SRR10151923,SRX6877430,SRS5413212,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s364,GSM4087485,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s364,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087485,GSM4087485: lckgfp s364; Danio rerio; RNA Seq,GSM4087485,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087485,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s364_R1.fastq.gz lckgfp_s364_R2.fastq.gz,fastq fastq,552863400.0,2764317.0,GSM4087485 r1,0:100 1:100,A:156249646;C:120365964;G:119437753;T:156807349;N:2688,100,100,,,156249646,120365964,119437753,156807349,2688,SRX6877430,SRS5413212,SRA965286,GEO,Stanford University,2,0.89031,0.89577,0.17435,0.17553,0.96646,0.96688,0.55727,0.54994,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54755,SRR10151922,SRX6877429,SRS5413211,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s363,GSM4087484,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s363,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087484,GSM4087484: lckgfp s363; Danio rerio; RNA Seq,GSM4087484,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087484,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s363_R1.fastq.gz lckgfp_s363_R2.fastq.gz,fastq fastq,627214400.0,3136072.0,GSM4087484 r1,0:100 1:100,A:181218539;C:131944904;G:131936387;T:182111593;N:2977,100,100,,,181218539,131944904,131936387,182111593,2977,SRX6877429,SRS5413211,SRA965286,GEO,Stanford University,2,0.8898,0.89468,0.25549,0.25745,0.96629,0.96682,0.5524,0.5679,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54756,SRR10151921,SRX6877428,SRS5413210,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s362,GSM4087483,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s362,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087483,GSM4087483: lckgfp s362; Danio rerio; RNA Seq,GSM4087483,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087483,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s362_R1.fastq.gz lckgfp_s362_R2.fastq.gz,fastq fastq,85745400.0,428727.0,GSM4087483 r1,0:100 1:100,A:27389948;C:15262098;G:15349274;T:27743616;N:464,100,100,,,27389948,15262098,15349274,27743616,464,SRX6877428,SRS5413210,SRA965286,GEO,Stanford University,2,0.68527,0.70382,0.38779,0.40917,0.97954,0.97932,0.62335,0.62203,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54757,SRR10151920,SRX6877427,SRS5413209,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s361,GSM4087482,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s361,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087482,GSM4087482: lckgfp s361; Danio rerio; RNA Seq,GSM4087482,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087482,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s361_R1.fastq.gz lckgfp_s361_R2.fastq.gz,fastq fastq,195593800.0,977969.0,GSM4087482 r1,0:100 1:100,A:56644246;C:40806137;G:40753130;T:57389382;N:905,100,100,,,56644246,40806137,40753130,57389382,905,SRX6877427,SRS5413209,SRA965286,GEO,Stanford University,2,0.83027,0.84227,0.11997,0.12315,0.94821,0.9483,0.62568,0.63216,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54758,SRR10151919,SRX6877426,SRS5413208,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s360,GSM4087481,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s360,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087481,GSM4087481: lckgfp s360; Danio rerio; RNA Seq,GSM4087481,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087481,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s360_R1.fastq.gz lckgfp_s360_R2.fastq.gz,fastq fastq,67188800.0,335944.0,GSM4087481 r1,0:100 1:100,A:21206262;C:12841122;G:12703318;T:20437773;N:325,100,100,,,21206262,12841122,12703318,20437773,325,SRX6877426,SRS5413208,SRA965286,GEO,Stanford University,2,0.70901,0.72161,0.23299,0.23771,0.95694,0.95672,0.60159,0.58695,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54759,SRR10151726,SRX6877425,SRS5413207,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s167,GSM4087288,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s167,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087288,GSM4087288: lckgfp s167; Danio rerio; RNA Seq,GSM4087288,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087288,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s167_R1.fastq.gz lckgfp_s167_R2.fastq.gz,fastq fastq,134099800.0,670499.0,GSM4087288 r1,0:100 1:100,A:38437552;C:29081890;G:28575183;T:38004579;N:596,100,100,,,38437552,29081890,28575183,38004579,596,SRX6877425,SRS5413207,SRA965286,GEO,Stanford University,2,0.88231,0.88686,0.18966,0.19146,0.96609,0.96575,0.59186,0.58694,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54760,SRR10151725,SRX6877424,SRS5413206,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s166,GSM4087287,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s166,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087287,GSM4087287: lckgfp s166; Danio rerio; RNA Seq,GSM4087287,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087287,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s166_R1.fastq.gz lckgfp_s166_R2.fastq.gz,fastq fastq,318347200.0,1591736.0,GSM4087287 r1,0:100 1:100,A:94955561;C:67550017;G:65256279;T:90583701;N:1642,100,100,,,94955561,67550017,65256279,90583701,1642,SRX6877424,SRS5413206,SRA965286,GEO,Stanford University,2,0.79823,0.81128,0.10342,0.10628,0.9796,0.9796,0.67093,0.66512,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54761,SRR10151724,SRX6877423,SRS5413205,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s165,GSM4087286,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s165,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087286,GSM4087286: lckgfp s165; Danio rerio; RNA Seq,GSM4087286,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087286,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s165_R1.fastq.gz lckgfp_s165_R2.fastq.gz,fastq fastq,191716400.0,958582.0,GSM4087286 r1,0:100 1:100,A:59383270;C:36673248;G:35909976;T:59748924;N:982,100,100,,,59383270,36673248,35909976,59748924,982,SRX6877423,SRS5413205,SRA965286,GEO,Stanford University,2,0.78441,0.79326,0.33307,0.33886,0.97281,0.97285,0.56961,0.56435,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54762,SRR10151723,SRX6877422,SRS5413204,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s164,GSM4087285,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s164,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087285,GSM4087285: lckgfp s164; Danio rerio; RNA Seq,GSM4087285,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087285,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s164_R1.fastq.gz lckgfp_s164_R2.fastq.gz,fastq fastq,149204800.0,746024.0,GSM4087285 r1,0:100 1:100,A:42851684;C:31730281;G:31227028;T:43395062;N:745,100,100,,,42851684,31730281,31227028,43395062,745,SRX6877422,SRS5413204,SRA965286,GEO,Stanford University,2,0.8756,0.88232,0.21262,0.21546,0.97212,0.97169,0.53497,0.54245,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54763,SRR10151722,SRX6877421,SRS5413203,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s163,GSM4087284,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s163,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087284,GSM4087284: lckgfp s163; Danio rerio; RNA Seq,GSM4087284,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087284,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s163_R1.fastq.gz lckgfp_s163_R2.fastq.gz,fastq fastq,277914000.0,1389570.0,GSM4087284 r1,0:100 1:100,A:82409899;C:58251309;G:57339795;T:79911604;N:1393,100,100,,,82409899,58251309,57339795,79911604,1393,SRX6877421,SRS5413203,SRA965286,GEO,Stanford University,2,0.76932,0.77938,0.13303,0.13733,0.9707,0.97092,0.62097,0.53929,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54764,SRR10151721,SRX6877420,SRS5413202,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s162,GSM4087283,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s162,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087283,GSM4087283: lckgfp s162; Danio rerio; RNA Seq,GSM4087283,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087283,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s162_R1.fastq.gz lckgfp_s162_R2.fastq.gz,fastq fastq,341944200.0,1709721.0,GSM4087283 r1,0:100 1:100,A:100250968;C:69296796;G:69902077;T:102492670;N:1689,100,100,,,100250968,69296796,69902077,102492670,1689,SRX6877420,SRS5413202,SRA965286,GEO,Stanford University,2,0.86953,0.87259,0.21619,0.21725,0.95954,0.95915,0.62694,0.6345,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54765,SRR10151720,SRX6877419,SRS5413201,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s161,GSM4087282,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s161,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087282,GSM4087282: lckgfp s161; Danio rerio; RNA Seq,GSM4087282,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087282,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s161_R1.fastq.gz lckgfp_s161_R2.fastq.gz,fastq fastq,264906400.0,1324532.0,GSM4087282 r1,0:100 1:100,A:80311157;C:49607384;G:50146596;T:84840148;N:1115,100,100,,,80311157,49607384,50146596,84840148,1115,SRX6877419,SRS5413201,SRA965286,GEO,Stanford University,2,0.76275,0.76861,0.28929,0.29961,0.97199,0.97165,0.57284,0.56866,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54766,SRR10151719,SRX6877418,SRS5413200,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s160,GSM4087281,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s160,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087281,GSM4087281: lckgfp s160; Danio rerio; RNA Seq,GSM4087281,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087281,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s160_R1.fastq.gz lckgfp_s160_R2.fastq.gz,fastq fastq,283260200.0,1416301.0,GSM4087281 r1,0:100 1:100,A:82604853;C:58139032;G:58184686;T:84330181;N:1448,100,100,,,82604853,58139032,58184686,84330181,1448,SRX6877418,SRS5413200,SRA965286,GEO,Stanford University,2,0.86656,0.87093,0.23748,0.23909,0.95933,0.95923,0.52555,0.52709,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54767,SRR10151718,SRX6877417,SRS5413199,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s159,GSM4087280,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s159,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087280,GSM4087280: lckgfp s159; Danio rerio; RNA Seq,GSM4087280,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087280,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s159_R1.fastq.gz lckgfp_s159_R2.fastq.gz,fastq fastq,113585400.0,567927.0,GSM4087280 r1,0:100 1:100,A:33517264;C:24562626;G:23339275;T:32165724;N:511,100,100,,,33517264,24562626,23339275,32165724,511,SRX6877417,SRS5413199,SRA965286,GEO,Stanford University,2,0.76624,0.77949,0.1336,0.13755,0.97571,0.97605,0.68103,0.67648,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54768,SRR10151717,SRX6877416,SRS5413198,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s158,GSM4087279,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s158,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087279,GSM4087279: lckgfp s158; Danio rerio; RNA Seq,GSM4087279,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087279,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s158_R1.fastq.gz lckgfp_s158_R2.fastq.gz,fastq fastq,399993200.0,1999966.0,GSM4087279 r1,0:100 1:100,A:117130575;C:84505609;G:85131263;T:113223731;N:2022,100,100,,,117130575,84505609,85131263,113223731,2022,SRX6877416,SRS5413198,SRA965286,GEO,Stanford University,2,0.82036,0.83218,0.14448,0.14619,0.9796,0.97956,0.62613,0.62015,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54769,SRR10151716,SRX6877415,SRS5413197,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s157,GSM4087278,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s157,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087278,GSM4087278: lckgfp s157; Danio rerio; RNA Seq,GSM4087278,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087278,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s157_R1.fastq.gz lckgfp_s157_R2.fastq.gz,fastq fastq,398558200.0,1992791.0,GSM4087278 r1,0:100 1:100,A:111681868;C:86694123;G:85997723;T:114182532;N:1954,100,100,,,111681868,86694123,85997723,114182532,1954,SRX6877415,SRS5413197,SRA965286,GEO,Stanford University,2,0.90085,0.90428,0.14906,0.1514,0.96104,0.9611,0.53613,0.53319,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54770,SRR10151715,SRX6877414,SRS5413196,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s156,GSM4087277,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s156,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087277,GSM4087277: lckgfp s156; Danio rerio; RNA Seq,GSM4087277,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087277,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s156_R1.fastq.gz lckgfp_s156_R2.fastq.gz,fastq fastq,328941200.0,1644706.0,GSM4087277 r1,0:100 1:100,A:92399112;C:71662966;G:71210741;T:93666738;N:1643,100,100,,,92399112,71662966,71210741,93666738,1643,SRX6877414,SRS5413196,SRA965286,GEO,Stanford University,2,0.89771,0.90148,0.1272,0.12826,0.96355,0.96351,0.56142,0.55489,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54771,SRR10151714,SRX6877413,SRS5413195,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s155,GSM4087276,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s155,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087276,GSM4087276: lckgfp s155; Danio rerio; RNA Seq,GSM4087276,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087276,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s155_R1.fastq.gz lckgfp_s155_R2.fastq.gz,fastq fastq,94356200.0,471781.0,GSM4087276 r1,0:100 1:100,A:28836770;C:20019385;G:19335070;T:26164477;N:498,100,100,,,28836770,20019385,19335070,26164477,498,SRX6877413,SRS5413195,SRA965286,GEO,Stanford University,2,0.66835,0.68158,0.18101,0.18641,0.97906,0.97837,0.71244,0.7043,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54772,SRR10151713,SRX6877412,SRS5413194,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s154,GSM4087275,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s154,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087275,GSM4087275: lckgfp s154; Danio rerio; RNA Seq,GSM4087275,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087275,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s154_R1.fastq.gz lckgfp_s154_R2.fastq.gz,fastq fastq,347199000.0,1735995.0,GSM4087275 r1,0:100 1:100,A:100034656;C:73316254;G:72860794;T:100985563;N:1733,100,100,,,100034656,73316254,72860794,100985563,1733,SRX6877412,SRS5413194,SRA965286,GEO,Stanford University,2,0.88154,0.88494,0.21058,0.21197,0.97307,0.97307,0.54664,0.54437,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54773,SRR10151712,SRX6877411,SRS5413193,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s153,GSM4087274,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s153,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087274,GSM4087274: lckgfp s153; Danio rerio; RNA Seq,GSM4087274,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087274,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s153_R1.fastq.gz lckgfp_s153_R2.fastq.gz,fastq fastq,62311800.0,311559.0,GSM4087274 r1,0:100 1:100,A:18913849;C:11367655;G:11408908;T:20621068;N:320,100,100,,,18913849,11367655,11408908,20621068,320,SRX6877411,SRS5413193,SRA965286,GEO,Stanford University,2,0.71644,0.7248,0.26054,0.2637,0.92533,0.92462,0.51549,0.51743,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54774,SRR10151711,SRX6877410,SRS5413192,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s152,GSM4087273,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s152,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087273,GSM4087273: lckgfp s152; Danio rerio; RNA Seq,GSM4087273,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087273,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s152_R1.fastq.gz lckgfp_s152_R2.fastq.gz,fastq fastq,144238600.0,721193.0,GSM4087273 r1,0:100 1:100,A:45120486;C:26673395;G:26068858;T:46375099;N:762,100,100,,,45120486,26673395,26068858,46375099,762,SRX6877410,SRS5413192,SRA965286,GEO,Stanford University,2,0.71878,0.73535,0.28355,0.29314,0.97341,0.97356,0.57729,0.50541,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54775,SRR10151710,SRX6877409,SRS5413191,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s151,GSM4087272,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s151,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087272,GSM4087272: lckgfp s151; Danio rerio; RNA Seq,GSM4087272,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087272,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s151_R1.fastq.gz lckgfp_s151_R2.fastq.gz,fastq fastq,146641800.0,733209.0,GSM4087272 r1,0:100 1:100,A:45410213;C:27128910;G:26872793;T:47229161;N:723,100,100,,,45410213,27128910,26872793,47229161,723,SRX6877409,SRS5413191,SRA965286,GEO,Stanford University,2,0.73931,0.75266,0.32123,0.3278,0.97177,0.97181,0.57725,0.56644,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54776,SRR10151709,SRX6877408,SRS5413190,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s150,GSM4087271,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s150,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087271,GSM4087271: lckgfp s150; Danio rerio; RNA Seq,GSM4087271,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087271,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s150_R1.fastq.gz lckgfp_s150_R2.fastq.gz,fastq fastq,255337000.0,1276685.0,GSM4087271 r1,0:100 1:100,A:75226278;C:50196924;G:50996568;T:78916258;N:972,100,100,,,75226278,50196924,50996568,78916258,972,SRX6877408,SRS5413190,SRA965286,GEO,Stanford University,2,0.86043,0.8626,0.15419,0.16488,0.97928,0.97962,0.51211,0.51506,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54777,SRR10151708,SRX6877407,SRS5413189,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s149,GSM4087270,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s149,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087270,GSM4087270: lckgfp s149; Danio rerio; RNA Seq,GSM4087270,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087270,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s149_R1.fastq.gz lckgfp_s149_R2.fastq.gz,fastq fastq,194156400.0,970782.0,GSM4087270 r1,0:100 1:100,A:59897924;C:39615168;G:39190310;T:55452086;N:912,100,100,,,59897924,39615168,39190310,55452086,912,SRX6877407,SRS5413189,SRA965286,GEO,Stanford University,2,0.73197,0.7347,0.21099,0.21268,0.97973,0.97987,0.63199,0.6265,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54778,SRR10151707,SRX6877406,SRS5413188,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s148,GSM4087269,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s148,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087269,GSM4087269: lckgfp s148; Danio rerio; RNA Seq,GSM4087269,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087269,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s148_R1.fastq.gz lckgfp_s148_R2.fastq.gz,fastq fastq,414642800.0,2073214.0,GSM4087269 r1,0:100 1:100,A:119142848;C:88002543;G:88261993;T:119233331;N:2085,100,100,,,119142848,88002543,88261993,119233331,2085,SRX6877406,SRS5413188,SRA965286,GEO,Stanford University,2,0.8558,0.86372,0.2333,0.23687,0.97656,0.97709,0.48822,0.48602,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54779,SRR10151706,SRX6877405,SRS5413187,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s147,GSM4087268,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s147,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087268,GSM4087268: lckgfp s147; Danio rerio; RNA Seq,GSM4087268,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087268,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s147_R1.fastq.gz lckgfp_s147_R2.fastq.gz,fastq fastq,43288800.0,216444.0,GSM4087268 r1,0:100 1:100,A:13740048;C:8211542;G:7907067;T:13429939;N:204,100,100,,,13740048,8211542,7907067,13429939,204,SRX6877405,SRS5413187,SRA965286,GEO,Stanford University,2,0.74681,0.76138,0.2961,0.30306,0.97912,0.97908,0.57322,0.56744,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54780,SRR10151705,SRX6877404,SRS5413186,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s146,GSM4087267,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s146,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087267,GSM4087267: lckgfp s146; Danio rerio; RNA Seq,GSM4087267,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087267,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s146_R1.fastq.gz lckgfp_s146_R2.fastq.gz,fastq fastq,97216400.0,486082.0,GSM4087267 r1,0:100 1:100,A:29646425;C:19960075;G:19334976;T:28274446;N:478,100,100,,,29646425,19960075,19334976,28274446,478,SRX6877404,SRS5413186,SRA965286,GEO,Stanford University,2,0.71587,0.72921,0.34087,0.34957,0.97926,0.97936,0.76031,0.76324,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54781,SRR10151704,SRX6877403,SRS5413185,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s145,GSM4087266,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s145,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087266,GSM4087266: lckgfp s145; Danio rerio; RNA Seq,GSM4087266,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087266,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s145_R1.fastq.gz lckgfp_s145_R2.fastq.gz,fastq fastq,379322000.0,1896610.0,GSM4087266 r1,0:100 1:100,A:109353830;C:80413955;G:79872676;T:109679806;N:1733,100,100,,,109353830,80413955,79872676,109679806,1733,SRX6877403,SRS5413185,SRA965286,GEO,Stanford University,2,0.87709,0.87951,0.19705,0.199,0.97362,0.97382,0.5766,0.56878,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54782,SRR10151703,SRX6877402,SRS5413184,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s144,GSM4087265,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s144,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087265,GSM4087265: lckgfp s144; Danio rerio; RNA Seq,GSM4087265,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087265,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s144_R1.fastq.gz lckgfp_s144_R2.fastq.gz,fastq fastq,231020000.0,1155100.0,GSM4087265 r1,0:100 1:100,A:77379294;C:43768856;G:42326505;T:67544213;N:1132,100,100,,,77379294,43768856,42326505,67544213,1132,SRX6877402,SRS5413184,SRA965286,GEO,Stanford University,2,0.49506,0.51863,0.10183,0.10619,0.97839,0.97873,0.58601,0.57166,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54783,SRR10151822,SRX6877401,SRS5413183,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s263,GSM4087384,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s263,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087384,GSM4087384: lckgfp s263; Danio rerio; RNA Seq,GSM4087384,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087384,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s263_R1.fastq.gz lckgfp_s263_R2.fastq.gz,fastq fastq,712463800.0,3562319.0,GSM4087384 r1,0:100 1:100,A:205721507;C:152468113;G:151554984;T:202715680;N:3516,100,100,,,205721507,152468113,151554984,202715680,3516,SRX6877401,SRS5413183,SRA965286,GEO,Stanford University,2,0.88497,0.88733,0.22509,0.22728,0.97743,0.97808,0.50855,0.50538,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54784,SRR10151821,SRX6877400,SRS5413182,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s262,GSM4087383,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s262,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087383,GSM4087383: lckgfp s262; Danio rerio; RNA Seq,GSM4087383,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087383,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s262_R1.fastq.gz lckgfp_s262_R2.fastq.gz,fastq fastq,178675400.0,893377.0,GSM4087383 r1,0:100 1:100,A:53088541;C:37211458;G:36084309;T:52290116;N:976,100,100,,,53088541,37211458,36084309,52290116,976,SRX6877400,SRS5413182,SRA965286,GEO,Stanford University,2,0.79432,0.80429,0.12839,0.13011,0.95239,0.95164,0.61717,0.61618,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54785,SRR10151820,SRX6877399,SRS5413181,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s261,GSM4087382,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s261,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087382,GSM4087382: lckgfp s261; Danio rerio; RNA Seq,GSM4087382,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087382,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s261_R1.fastq.gz lckgfp_s261_R2.fastq.gz,fastq fastq,252768600.0,1263843.0,GSM4087382 r1,0:100 1:100,A:75518513;C:48713471;G:48832901;T:79702638;N:1077,100,100,,,75518513,48713471,48832901,79702638,1077,SRX6877399,SRS5413181,SRA965286,GEO,Stanford University,2,0.77679,0.78282,0.25516,0.25959,0.95732,0.95755,0.58772,0.5841,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54786,SRR10151819,SRX6877398,SRS5413180,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s260,GSM4087381,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s260,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087381,GSM4087381: lckgfp s260; Danio rerio; RNA Seq,GSM4087381,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087381,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s260_R1.fastq.gz lckgfp_s260_R2.fastq.gz,fastq fastq,640574600.0,3202873.0,GSM4087381 r1,0:100 1:100,A:193610199;C:125516191;G:124583853;T:196860953;N:3404,100,100,,,193610199,125516191,124583853,196860953,3404,SRX6877398,SRS5413180,SRA965286,GEO,Stanford University,2,0.83935,0.84602,0.40709,0.41161,0.97516,0.97475,0.57799,0.56883,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54787,SRR10151818,SRX6877397,SRS5413179,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s259,GSM4087380,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s259,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087380,GSM4087380: lckgfp s259; Danio rerio; RNA Seq,GSM4087380,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087380,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s259_R1.fastq.gz lckgfp_s259_R2.fastq.gz,fastq fastq,442001600.0,2210008.0,GSM4087380 r1,0:100 1:100,A:132718375;C:93145071;G:89720970;T:126414987;N:2197,100,100,,,132718375,93145071,89720970,126414987,2197,SRX6877397,SRS5413179,SRA965286,GEO,Stanford University,2,0.7658,0.77463,0.22923,0.23214,0.97741,0.97804,0.53957,0.533,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54788,SRR10151817,SRX6877396,SRS5413178,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s258,GSM4087379,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s258,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087379,GSM4087379: lckgfp s258; Danio rerio; RNA Seq,GSM4087379,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087379,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s258_R1.fastq.gz lckgfp_s258_R2.fastq.gz,fastq fastq,768891200.0,3844456.0,GSM4087379 r1,0:100 1:100,A:221919805;C:160432858;G:160462157;T:226073019;N:3361,100,100,,,221919805,160432858,160462157,226073019,3361,SRX6877396,SRS5413178,SRA965286,GEO,Stanford University,2,0.87353,0.87502,0.19594,0.203,0.98344,0.98309,0.59672,0.60437,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54789,SRR10151816,SRX6877395,SRS5413177,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s257,GSM4087378,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s257,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087378,GSM4087378: lckgfp s257; Danio rerio; RNA Seq,GSM4087378,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087378,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s257_R1.fastq.gz lckgfp_s257_R2.fastq.gz,fastq fastq,111123800.0,555619.0,GSM4087378 r1,0:100 1:100,A:33584827;C:23346349;G:22705440;T:31486575;N:609,100,100,,,33584827,23346349,22705440,31486575,609,SRX6877395,SRS5413177,SRA965286,GEO,Stanford University,2,0.77078,0.78615,0.12471,0.12764,0.9825,0.9822,0.64769,0.64665,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54790,SRR10151815,SRX6877394,SRS5413176,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s256,GSM4087377,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s256,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087377,GSM4087377: lckgfp s256; Danio rerio; RNA Seq,GSM4087377,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087377,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s256_R1.fastq.gz lckgfp_s256_R2.fastq.gz,fastq fastq,224647200.0,1123236.0,GSM4087377 r1,0:100 1:100,A:64126817;C:48580286;G:48138688;T:63800313;N:1096,100,100,,,64126817,48580286,48138688,63800313,1096,SRX6877394,SRS5413176,SRA965286,GEO,Stanford University,2,0.88362,0.88769,0.14051,0.14263,0.96262,0.9629,0.56492,0.5715,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54791,SRR10151814,SRX6877393,SRS5413175,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s255,GSM4087376,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s255,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087376,GSM4087376: lckgfp s255; Danio rerio; RNA Seq,GSM4087376,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087376,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s255_R1.fastq.gz lckgfp_s255_R2.fastq.gz,fastq fastq,638629200.0,3193146.0,GSM4087376 r1,0:100 1:100,A:180213037;C:137091196;G:136887885;T:184433891;N:3191,100,100,,,180213037,137091196,136887885,184433891,3191,SRX6877393,SRS5413175,SRA965286,GEO,Stanford University,2,0.89004,0.89579,0.16231,0.16498,0.95645,0.95639,0.4788,0.55779,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54792,SRR10151813,SRX6877392,SRS5413174,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s254,GSM4087375,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s254,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087375,GSM4087375: lckgfp s254; Danio rerio; RNA Seq,GSM4087375,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087375,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s254_R1.fastq.gz lckgfp_s254_R2.fastq.gz,fastq fastq,31360400.0,156802.0,GSM4087375 r1,0:100 1:100,A:8827768;C:7340349;G:6501576;T:8690590;N:117,100,100,,,8827768,7340349,6501576,8690590,117,SRX6877392,SRS5413174,SRA965286,GEO,Stanford University,2,0.83031,0.82256,0.25236,0.25193,0.9609,0.96051,0.61835,0.62023,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54793,SRR10151812,SRX6877391,SRS5413173,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s253,GSM4087374,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s253,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087374,GSM4087374: lckgfp s253; Danio rerio; RNA Seq,GSM4087374,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087374,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s253_R1.fastq.gz lckgfp_s253_R2.fastq.gz,fastq fastq,278116800.0,1390584.0,GSM4087374 r1,0:100 1:100,A:81621196;C:58531356;G:57679579;T:80283279;N:1390,100,100,,,81621196,58531356,57679579,80283279,1390,SRX6877391,SRS5413173,SRA965286,GEO,Stanford University,2,0.82554,0.83184,0.18026,0.18251,0.97313,0.97374,0.62647,0.62099,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54794,SRR10151811,SRX6877390,SRS5413172,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s252,GSM4087373,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s252,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087373,GSM4087373: lckgfp s252; Danio rerio; RNA Seq,GSM4087373,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087373,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s252_R1.fastq.gz lckgfp_s252_R2.fastq.gz,fastq fastq,357782000.0,1788910.0,GSM4087373 r1,0:100 1:100,A:100211591;C:79953356;G:78673775;T:98941609;N:1669,100,100,,,100211591,79953356,78673775,98941609,1669,SRX6877390,SRS5413172,SRA965286,GEO,Stanford University,2,0.90178,0.90652,0.19325,0.19452,0.97281,0.9727,0.46592,0.55759,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54795,SRR10151810,SRX6877389,SRS5413171,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s251,GSM4087372,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s251,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087372,GSM4087372: lckgfp s251; Danio rerio; RNA Seq,GSM4087372,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087372,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s251_R1.fastq.gz lckgfp_s251_R2.fastq.gz,fastq fastq,154662200.0,773311.0,GSM4087372 r1,0:100 1:100,A:46195763;C:30904197;G:30540939;T:47020511;N:790,100,100,,,46195763,30904197,30540939,47020511,790,SRX6877389,SRS5413171,SRA965286,GEO,Stanford University,2,0.77199,0.78625,0.15104,0.15562,0.94251,0.94312,0.64877,0.65251,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54796,SRR10151809,SRX6877388,SRS5413170,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s250,GSM4087371,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s250,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087371,GSM4087371: lckgfp s250; Danio rerio; RNA Seq,GSM4087371,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087371,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s250_R1.fastq.gz lckgfp_s250_R2.fastq.gz,fastq fastq,209954800.0,1049774.0,GSM4087371 r1,0:100 1:100,A:64870402;C:41383363;G:40467851;T:63232087;N:1097,100,100,,,64870402,41383363,40467851,63232087,1097,SRX6877388,SRS5413170,SRA965286,GEO,Stanford University,2,0.70643,0.71876,0.36885,0.37692,0.97751,0.97757,0.66872,0.6539,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54797,SRR10151808,SRX6877387,SRS5413169,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s249,GSM4087370,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s249,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087370,GSM4087370: lckgfp s249; Danio rerio; RNA Seq,GSM4087370,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087370,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s249_R1.fastq.gz lckgfp_s249_R2.fastq.gz,fastq fastq,300503400.0,1502517.0,GSM4087370 r1,0:100 1:100,A:87918930;C:64039264;G:62110563;T:86433086;N:1557,100,100,,,87918930,64039264,62110563,86433086,1557,SRX6877387,SRS5413169,SRA965286,GEO,Stanford University,2,0.80576,0.81508,0.13364,0.13617,0.98001,0.98023,0.5399,0.53423,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54798,SRR10151807,SRX6877386,SRS5413168,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s248,GSM4087369,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s248,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087369,GSM4087369: lckgfp s248; Danio rerio; RNA Seq,GSM4087369,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087369,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s248_R1.fastq.gz lckgfp_s248_R2.fastq.gz,fastq fastq,508455000.0,2542275.0,GSM4087369 r1,0:100 1:100,A:145063832;C:108777134;G:107969639;T:146641852;N:2543,100,100,,,145063832,108777134,107969639,146641852,2543,SRX6877386,SRS5413168,SRA965286,GEO,Stanford University,2,0.87661,0.87906,0.17214,0.17469,0.97007,0.97021,0.54058,0.54793,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54799,SRR10151806,SRX6877385,SRS5413167,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s247,GSM4087368,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s247,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087368,GSM4087368: lckgfp s247; Danio rerio; RNA Seq,GSM4087368,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087368,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s247_R1.fastq.gz lckgfp_s247_R2.fastq.gz,fastq fastq,503439800.0,2517199.0,GSM4087368 r1,0:100 1:100,A:144603360;C:106681907;G:107064271;T:145087767;N:2495,100,100,,,144603360,106681907,107064271,145087767,2495,SRX6877385,SRS5413167,SRA965286,GEO,Stanford University,2,0.90271,0.90502,0.16525,0.16592,0.96958,0.96988,0.53789,0.55192,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54800,SRR10151805,SRX6877384,SRS5413166,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s246,GSM4087367,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s246,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087367,GSM4087367: lckgfp s246; Danio rerio; RNA Seq,GSM4087367,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087367,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s246_R1.fastq.gz lckgfp_s246_R2.fastq.gz,fastq fastq,56423000.0,282115.0,GSM4087367 r1,0:100 1:100,A:16703198;C:11821345;G:11582009;T:16316138;N:310,100,100,,,16703198,11821345,11582009,16316138,310,SRX6877384,SRS5413166,SRA965286,GEO,Stanford University,2,0.86004,0.86722,0.20783,0.2103,0.97437,0.97413,0.52979,0.52929,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54801,SRR10151804,SRX6877383,SRS5413165,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s245,GSM4087366,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s245,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087366,GSM4087366: lckgfp s245; Danio rerio; RNA Seq,GSM4087366,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087366,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s245_R1.fastq.gz lckgfp_s245_R2.fastq.gz,fastq fastq,199249200.0,996246.0,GSM4087366 r1,0:100 1:100,A:63853905;C:39801278;G:38619900;T:56973038;N:1079,100,100,,,63853905,39801278,38619900,56973038,1079,SRX6877383,SRS5413165,SRA965286,GEO,Stanford University,2,0.69175,0.70899,0.18871,0.19499,0.98248,0.98265,0.69347,0.69228,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54802,SRR10151803,SRX6877382,SRS5413164,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s244,GSM4087365,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s244,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087365,GSM4087365: lckgfp s244; Danio rerio; RNA Seq,GSM4087365,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087365,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s244_R1.fastq.gz lckgfp_s244_R2.fastq.gz,fastq fastq,59458000.0,297290.0,GSM4087365 r1,0:100 1:100,A:19368446;C:11883955;G:11060031;T:17145254;N:314,100,100,,,19368446,11883955,11060031,17145254,314,SRX6877382,SRS5413164,SRA965286,GEO,Stanford University,2,0.64861,0.67048,0.17158,0.17966,0.98013,0.98098,0.67747,0.67468,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54803,SRR10151802,SRX6877381,SRS5413163,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s243,GSM4087364,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s243,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087364,GSM4087364: lckgfp s243; Danio rerio; RNA Seq,GSM4087364,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087364,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s243_R1.fastq.gz lckgfp_s243_R2.fastq.gz,fastq fastq,912862800.0,4564314.0,GSM4087364 r1,0:100 1:100,A:256150630;C:198146668;G:198794207;T:259767092;N:4203,100,100,,,256150630,198146668,198794207,259767092,4203,SRX6877381,SRS5413163,SRA965286,GEO,Stanford University,2,0.90111,0.9029,0.16436,0.16741,0.95321,0.95229,0.54584,0.54742,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54804,SRR10151801,SRX6877380,SRS5413162,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s242,GSM4087363,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s242,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087363,GSM4087363: lckgfp s242; Danio rerio; RNA Seq,GSM4087363,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087363,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s242_R1.fastq.gz lckgfp_s242_R2.fastq.gz,fastq fastq,212411000.0,1062055.0,GSM4087363 r1,0:100 1:100,A:64672691;C:44589172;G:41615063;T:61532914;N:1160,100,100,,,64672691,44589172,41615063,61532914,1160,SRX6877380,SRS5413162,SRA965286,GEO,Stanford University,2,0.62044,0.63602,0.48721,0.49958,0.96404,0.96404,0.56834,0.53787,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54805,SRR10151800,SRX6877379,SRS5413161,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s241,GSM4087362,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s241,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087362,GSM4087362: lckgfp s241; Danio rerio; RNA Seq,GSM4087362,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087362,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s241_R1.fastq.gz lckgfp_s241_R2.fastq.gz,fastq fastq,222790000.0,1113950.0,GSM4087362 r1,0:100 1:100,A:67590311;C:41984958;G:41881875;T:71331643;N:1213,100,100,,,67590311,41984958,41881875,71331643,1213,SRX6877379,SRS5413161,SRA965286,GEO,Stanford University,2,0.77605,0.78297,0.28043,0.28651,0.97538,0.97557,0.53532,0.57878,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54806,SRR10151799,SRX6877378,SRS5413160,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s240,GSM4087361,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s240,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087361,GSM4087361: lckgfp s240; Danio rerio; RNA Seq,GSM4087361,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087361,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s240_R1.fastq.gz lckgfp_s240_R2.fastq.gz,fastq fastq,153466400.0,767332.0,GSM4087361 r1,0:100 1:100,A:50048852;C:28704914;G:28416813;T:46295033;N:788,100,100,,,50048852,28704914,28416813,46295033,788,SRX6877378,SRS5413160,SRA965286,GEO,Stanford University,2,0.71293,0.72959,0.31443,0.32369,0.97966,0.98015,0.71821,0.7056,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54807,SRR10151918,SRX6877377,SRS5413159,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s359,GSM4087480,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s359,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087480,GSM4087480: lckgfp s359; Danio rerio; RNA Seq,GSM4087480,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087480,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s359_R1.fastq.gz lckgfp_s359_R2.fastq.gz,fastq fastq,180259400.0,901297.0,GSM4087480 r1,0:100 1:100,A:51414242;C:38692238;G:38347124;T:51804826;N:970,100,100,,,51414242,38692238,38347124,51804826,970,SRX6877377,SRS5413159,SRA965286,GEO,Stanford University,2,0.88833,0.89291,0.23149,0.23212,0.97047,0.97088,0.49045,0.55701,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54808,SRR10151917,SRX6877376,SRS5413158,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s358,GSM4087479,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s358,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087479,GSM4087479: lckgfp s358; Danio rerio; RNA Seq,GSM4087479,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087479,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s358_R1.fastq.gz lckgfp_s358_R2.fastq.gz,fastq fastq,375455400.0,1877277.0,GSM4087479 r1,0:100 1:100,A:109265882;C:78761598;G:77114775;T:110311304;N:1841,100,100,,,109265882,78761598,77114775,110311304,1841,SRX6877376,SRS5413158,SRA965286,GEO,Stanford University,2,0.86409,0.86748,0.23888,0.24065,0.96897,0.96919,0.57659,0.56636,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54809,SRR10151916,SRX6877375,SRS5413157,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s357,GSM4087478,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s357,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087478,GSM4087478: lckgfp s357; Danio rerio; RNA Seq,GSM4087478,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087478,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s357_R1.fastq.gz lckgfp_s357_R2.fastq.gz,fastq fastq,1031071600.0,5155358.0,GSM4087478 r1,0:100 1:100,A:290286165;C:220464752;G:221676322;T:298639325;N:5036,100,100,,,290286165,220464752,221676322,298639325,5036,SRX6877375,SRS5413157,SRA965286,GEO,Stanford University,2,0.89351,0.8959,0.15814,0.15969,0.95483,0.95501,0.5582,0.55541,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54810,SRR10151915,SRX6877374,SRS5413156,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s356,GSM4087477,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s356,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087477,GSM4087477: lckgfp s356; Danio rerio; RNA Seq,GSM4087477,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087477,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s356_R1.fastq.gz lckgfp_s356_R2.fastq.gz,fastq fastq,631604600.0,3158023.0,GSM4087477 r1,0:100 1:100,A:179705284;C:135901184;G:136090966;T:179904181;N:2985,100,100,,,179705284,135901184,136090966,179904181,2985,SRX6877374,SRS5413156,SRA965286,GEO,Stanford University,2,0.89013,0.89373,0.16216,0.16397,0.97159,0.97153,0.52157,0.52159,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54811,SRR10151914,SRX6877373,SRS5413155,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s355,GSM4087476,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s355,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087476,GSM4087476: lckgfp s355; Danio rerio; RNA Seq,GSM4087476,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087476,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s355_R1.fastq.gz lckgfp_s355_R2.fastq.gz,fastq fastq,437755400.0,2188777.0,GSM4087476 r1,0:100 1:100,A:125630217;C:93000635;G:93133725;T:125988715;N:2108,100,100,,,125630217,93000635,93133725,125988715,2108,SRX6877373,SRS5413155,SRA965286,GEO,Stanford University,2,0.8771,0.88183,0.22446,0.22704,0.97682,0.97715,0.51148,0.51062,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54812,SRR10151913,SRX6877372,SRS5413154,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s354,GSM4087475,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s354,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087475,GSM4087475: lckgfp s354; Danio rerio; RNA Seq,GSM4087475,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087475,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s354_R1.fastq.gz lckgfp_s354_R2.fastq.gz,fastq fastq,187430200.0,937151.0,GSM4087475 r1,0:100 1:100,A:56210423;C:40204731;G:37870530;T:53143658;N:858,100,100,,,56210423,40204731,37870530,53143658,858,SRX6877372,SRS5413154,SRA965286,GEO,Stanford University,2,0.79366,0.81071,0.12291,0.12589,0.98033,0.98031,0.6249,0.63056,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54813,SRR10151912,SRX6877371,SRS5413153,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s353,GSM4087474,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s353,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087474,GSM4087474: lckgfp s353; Danio rerio; RNA Seq,GSM4087474,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087474,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s353_R1.fastq.gz lckgfp_s353_R2.fastq.gz,fastq fastq,288086200.0,1440431.0,GSM4087474 r1,0:100 1:100,A:82382315;C:61117555;G:61270028;T:83314923;N:1379,100,100,,,82382315,61117555,61270028,83314923,1379,SRX6877371,SRS5413153,SRA965286,GEO,Stanford University,2,0.87548,0.87893,0.21375,0.21654,0.96678,0.96633,0.49046,0.4943,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54814,SRR10151911,SRX6877370,SRS5413152,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s352,GSM4087473,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s352,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087473,GSM4087473: lckgfp s352; Danio rerio; RNA Seq,GSM4087473,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087473,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s352_R1.fastq.gz lckgfp_s352_R2.fastq.gz,fastq fastq,234676200.0,1173381.0,GSM4087473 r1,0:100 1:100,A:71012748;C:48194679;G:47384221;T:68083483;N:1069,100,100,,,71012748,48194679,47384221,68083483,1069,SRX6877370,SRS5413152,SRA965286,GEO,Stanford University,2,0.78896,0.80572,0.16654,0.17056,0.97761,0.97753,0.58617,0.5841,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54815,SRR10151910,SRX6877369,SRS5413151,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s351,GSM4087472,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s351,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087472,GSM4087472: lckgfp s351; Danio rerio; RNA Seq,GSM4087472,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087472,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s351_R1.fastq.gz lckgfp_s351_R2.fastq.gz,fastq fastq,142332600.0,711663.0,GSM4087472 r1,0:100 1:100,A:46730887;C:27992765;G:26483796;T:41124486;N:666,100,100,,,46730887,27992765,26483796,41124486,666,SRX6877369,SRS5413151,SRA965286,GEO,Stanford University,2,0.63313,0.65934,0.17905,0.18805,0.98397,0.98429,0.68129,0.67934,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54816,SRR10151909,SRX6877368,SRS5413150,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s350,GSM4087471,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s350,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087471,GSM4087471: lckgfp s350; Danio rerio; RNA Seq,GSM4087471,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087471,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s350_R1.fastq.gz lckgfp_s350_R2.fastq.gz,fastq fastq,256653200.0,1283266.0,GSM4087471 r1,0:100 1:100,A:79486976;C:53119381;G:50178393;T:73867234;N:1216,100,100,,,79486976,53119381,50178393,73867234,1216,SRX6877368,SRS5413150,SRA965286,GEO,Stanford University,2,0.74257,0.75866,0.1224,0.12466,0.97693,0.97688,0.64957,0.65551,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54817,SRR10151908,SRX6877367,SRS5413149,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s349,GSM4087470,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s349,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087470,GSM4087470: lckgfp s349; Danio rerio; RNA Seq,GSM4087470,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087470,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s349_R1.fastq.gz lckgfp_s349_R2.fastq.gz,fastq fastq,266517000.0,1332585.0,GSM4087470 r1,0:100 1:100,A:78245242;C:50857180;G:52125469;T:85287928;N:1181,100,100,,,78245242,50857180,52125469,85287928,1181,SRX6877367,SRS5413149,SRA965286,GEO,Stanford University,2,0.8172,0.81942,0.22884,0.23527,0.95069,0.95043,0.53777,0.53346,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54818,SRR10151907,SRX6877366,SRS5413148,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s348,GSM4087469,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s348,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087469,GSM4087469: lckgfp s348; Danio rerio; RNA Seq,GSM4087469,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087469,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s348_R1.fastq.gz lckgfp_s348_R2.fastq.gz,fastq fastq,218351400.0,1091757.0,GSM4087469 r1,0:100 1:100,A:64900003;C:46681580;G:45663559;T:61105186;N:1072,100,100,,,64900003,46681580,45663559,61105186,1072,SRX6877366,SRS5413148,SRA965286,GEO,Stanford University,2,0.80696,0.81832,0.12677,0.12868,0.97798,0.97768,0.47529,0.47334,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54819,SRR10151906,SRX6877365,SRS5413147,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s347,GSM4087468,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s347,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087468,GSM4087468: lckgfp s347; Danio rerio; RNA Seq,GSM4087468,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087468,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s347_R1.fastq.gz lckgfp_s347_R2.fastq.gz,fastq fastq,1283436400.0,6417182.0,GSM4087468 r1,0:100 1:100,A:360512221;C:280178229;G:280104753;T:362636146;N:5051,100,100,,,360512221,280178229,280104753,362636146,5051,SRX6877365,SRS5413147,SRA965286,GEO,Stanford University,2,0.90384,0.90101,0.23515,0.23455,0.98307,0.9835,0.53235,0.60485,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54820,SRR10151905,SRX6877364,SRS5413146,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s346,GSM4087467,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s346,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087467,GSM4087467: lckgfp s346; Danio rerio; RNA Seq,GSM4087467,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087467,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s346_R1.fastq.gz lckgfp_s346_R2.fastq.gz,fastq fastq,265081400.0,1325407.0,GSM4087467 r1,0:100 1:100,A:77020683;C:56160067;G:55647714;T:76251684;N:1252,100,100,,,77020683,56160067,55647714,76251684,1252,SRX6877364,SRS5413146,SRA965286,GEO,Stanford University,2,0.88027,0.88557,0.22235,0.22374,0.9736,0.97337,0.53556,0.53951,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54821,SRR10151904,SRX6877363,SRS5413145,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s345,GSM4087466,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s345,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087466,GSM4087466: lckgfp s345; Danio rerio; RNA Seq,GSM4087466,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087466,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s345_R1.fastq.gz lckgfp_s345_R2.fastq.gz,fastq fastq,920847800.0,4604239.0,GSM4087466 r1,0:100 1:100,A:262122009;C:197882908;G:197475240;T:263362852;N:4791,100,100,,,262122009,197882908,197475240,263362852,4791,SRX6877363,SRS5413145,SRA965286,GEO,Stanford University,2,0.90511,0.90613,0.20408,0.20473,0.97388,0.97372,0.54732,0.53091,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54822,SRR10151903,SRX6877362,SRS5413144,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s344,GSM4087465,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s344,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087465,GSM4087465: lckgfp s344; Danio rerio; RNA Seq,GSM4087465,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087465,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s344_R1.fastq.gz lckgfp_s344_R2.fastq.gz,fastq fastq,397396000.0,1986980.0,GSM4087465 r1,0:100 1:100,A:114048468;C:83076003;G:83782458;T:116487410;N:1661,100,100,,,114048468,83076003,83782458,116487410,1661,SRX6877362,SRS5413144,SRA965286,GEO,Stanford University,2,0.87017,0.87046,0.17392,0.17754,0.96613,0.96658,0.52672,0.53224,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54823,SRR10151902,SRX6877361,SRS5413143,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s343,GSM4087464,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s343,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087464,GSM4087464: lckgfp s343; Danio rerio; RNA Seq,GSM4087464,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087464,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s343_R1.fastq.gz lckgfp_s343_R2.fastq.gz,fastq fastq,227875000.0,1139375.0,GSM4087464 r1,0:100 1:100,A:65818467;C:47807609;G:48005484;T:66242292;N:1148,100,100,,,65818467,47807609,48005484,66242292,1148,SRX6877361,SRS5413143,SRA965286,GEO,Stanford University,2,0.88242,0.88665,0.1815,0.18371,0.95649,0.95651,0.64558,0.64214,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54824,SRR10151901,SRX6877360,SRS5413142,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s342,GSM4087463,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s342,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087463,GSM4087463: lckgfp s342; Danio rerio; RNA Seq,GSM4087463,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087463,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s342_R1.fastq.gz lckgfp_s342_R2.fastq.gz,fastq fastq,326677200.0,1633386.0,GSM4087463 r1,0:100 1:100,A:98861522;C:68201149;G:66098299;T:93514645;N:1585,100,100,,,98861522,68201149,66098299,93514645,1585,SRX6877360,SRS5413142,SRA965286,GEO,Stanford University,2,0.78217,0.79465,0.12683,0.12957,0.97638,0.97626,0.6703,0.66036,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54825,SRR10151900,SRX6877359,SRS5413141,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s341,GSM4087462,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s341,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087462,GSM4087462: lckgfp s341; Danio rerio; RNA Seq,GSM4087462,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087462,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s341_R1.fastq.gz lckgfp_s341_R2.fastq.gz,fastq fastq,158634200.0,793171.0,GSM4087462 r1,0:100 1:100,A:46027823;C:33195724;G:32948463;T:46461412;N:778,100,100,,,46027823,33195724,32948463,46461412,778,SRX6877359,SRS5413141,SRA965286,GEO,Stanford University,2,0.85117,0.85623,0.27432,0.276,0.96648,0.96698,0.5827,0.59421,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54826,SRR10151899,SRX6877358,SRS5413140,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s340,GSM4087461,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s340,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087461,GSM4087461: lckgfp s340; Danio rerio; RNA Seq,GSM4087461,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087461,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s340_R1.fastq.gz lckgfp_s340_R2.fastq.gz,fastq fastq,568563200.0,2842816.0,GSM4087461 r1,0:100 1:100,A:165178313;C:115417192;G:117058241;T:170906676;N:2778,100,100,,,165178313,115417192,117058241,170906676,2778,SRX6877358,SRS5413140,SRA965286,GEO,Stanford University,2,0.87206,0.87619,0.2435,0.24485,0.94306,0.94343,0.55046,0.55054,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54827,SRR10151898,SRX6877357,SRS5413139,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s339,GSM4087460,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s339,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087460,GSM4087460: lckgfp s339; Danio rerio; RNA Seq,GSM4087460,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087460,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s339_R1.fastq.gz lckgfp_s339_R2.fastq.gz,fastq fastq,410629400.0,2053147.0,GSM4087460 r1,0:100 1:100,A:117642576;C:87662203;G:88629364;T:116693863;N:1394,100,100,,,117642576,87662203,88629364,116693863,1394,SRX6877357,SRS5413139,SRA965286,GEO,Stanford University,2,0.89777,0.90006,0.22532,0.22939,0.97699,0.9767,0.50735,0.51424,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54828,SRR10151897,SRX6877356,SRS5413138,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s338,GSM4087459,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s338,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087459,GSM4087459: lckgfp s338; Danio rerio; RNA Seq,GSM4087459,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087459,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s338_R1.fastq.gz lckgfp_s338_R2.fastq.gz,fastq fastq,263372800.0,1316864.0,GSM4087459 r1,0:100 1:100,A:78308088;C:55328404;G:54055536;T:75679420;N:1352,100,100,,,78308088,55328404,54055536,75679420,1352,SRX6877356,SRS5413138,SRA965286,GEO,Stanford University,2,0.78053,0.79444,0.13285,0.13649,0.97512,0.97481,0.61918,0.61456,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54829,SRR10151896,SRX6877355,SRS5413137,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s337,GSM4087458,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s337,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087458,GSM4087458: lckgfp s337; Danio rerio; RNA Seq,GSM4087458,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087458,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s337_R1.fastq.gz lckgfp_s337_R2.fastq.gz,fastq fastq,86229200.0,431146.0,GSM4087458 r1,0:100 1:100,A:25113545;C:18333224;G:17847043;T:24935027;N:361,100,100,,,25113545,18333224,17847043,24935027,361,SRX6877355,SRS5413137,SRA965286,GEO,Stanford University,2,0.85137,0.85805,0.15414,0.15581,0.97037,0.97037,0.53809,0.536,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54830,SRR10151895,SRX6877354,SRS5413136,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s336,GSM4087457,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s336,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087457,GSM4087457: lckgfp s336; Danio rerio; RNA Seq,GSM4087457,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087457,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s336_R1.fastq.gz lckgfp_s336_R2.fastq.gz,fastq fastq,121835000.0,609175.0,GSM4087457 r1,0:100 1:100,A:36889990;C:25693167;G:24506378;T:34744930;N:535,100,100,,,36889990,25693167,24506378,34744930,535,SRX6877354,SRS5413136,SRA965286,GEO,Stanford University,2,0.79594,0.81093,0.12049,0.12402,0.98025,0.9807,0.64969,0.53793,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54831,SRR10151894,SRX6877353,SRS5413135,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s335,GSM4087456,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s335,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087456,GSM4087456: lckgfp s335; Danio rerio; RNA Seq,GSM4087456,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087456,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s335_R1.fastq.gz lckgfp_s335_R2.fastq.gz,fastq fastq,520323800.0,2601619.0,GSM4087456 r1,0:100 1:100,A:153396258;C:105740250;G:106868108;T:154316562;N:2622,100,100,,,153396258,105740250,106868108,154316562,2622,SRX6877353,SRS5413135,SRA965286,GEO,Stanford University,2,0.88428,0.88569,0.22218,0.22303,0.97035,0.97068,0.63041,0.63561,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54832,SRR10151893,SRX6877352,SRS5413134,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s334,GSM4087455,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s334,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087455,GSM4087455: lckgfp s334; Danio rerio; RNA Seq,GSM4087455,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087455,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s334_R1.fastq.gz lckgfp_s334_R2.fastq.gz,fastq fastq,99728600.0,498643.0,GSM4087455 r1,0:100 1:100,A:31418598;C:17730571;G:17893486;T:32685468;N:477,100,100,,,31418598,17730571,17893486,32685468,477,SRX6877352,SRS5413134,SRA965286,GEO,Stanford University,2,0.68924,0.70061,0.30149,0.31093,0.97287,0.9735,0.55536,0.54729,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54833,SRR10151892,SRX6877351,SRS5413133,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s333,GSM4087454,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s333,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087454,GSM4087454: lckgfp s333; Danio rerio; RNA Seq,GSM4087454,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087454,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s333_R1.fastq.gz lckgfp_s333_R2.fastq.gz,fastq fastq,162143400.0,810717.0,GSM4087454 r1,0:100 1:100,A:49268783;C:29510657;G:29658985;T:53704074;N:901,100,100,,,49268783,29510657,29658985,53704074,901,SRX6877351,SRS5413133,SRA965286,GEO,Stanford University,2,0.74983,0.76023,0.2521,0.25783,0.96134,0.96132,0.56941,0.57083,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system 54834,SRR10151891,SRX6877350,SRS5413132,SRP222763,PRJNA572587,Characterization of T cells from the larval zebrafish tail via single cell RNAseq,GSE137770,Transcriptome Analysis,We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.,,pubmed:32427565,,lckgfp s332,GSM4087453,,source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,lckgfp s332,STAR 2.5 Htseq 0.8.0 htseq count m intersection nonempty nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell in tab separated format lckgfp counts.txt,Danio rerio tail,Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,,line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf,GSM4087453,GSM4087453: lckgfp s332; Danio rerio; RNA Seq,GSM4087453,,1,Lysis was performed as described in Schaum et al. Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification and a Nextera based protocol was used for library preparation as described in Schaum et al. Nature 2018,GEO Accession:GSM4087453,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP222763,,,lckgfp_s332_R1.fastq.gz lckgfp_s332_R2.fastq.gz,fastq fastq,149555600.0,747778.0,GSM4087453 r1,0:100 1:100,A:44567604;C:28000220;G:34850087;T:42136937;N:752,100,100,,,44567604,28000220,34850087,42136937,752,SRX6877350,SRS5413132,SRA965286,GEO,Stanford University,2,0.68734,0.7551,0.31102,0.32952,0.97224,0.97575,0.69437,0.70905,100,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,cdna_unspecified,nextera,sc,single_cell_plate,smartseq,,United States,2019-09-20,Larval,Larval,Tail,Multi-system