rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 0,DRR314108,DRX303511,DRS233566,DRP008373,PRJDB12134,Comparison of expression profile between banp mutant and wildtype sibling.,DRP008373,Other,To characterize the physiological function of Banp the expression profile of banp mutant and wild type sibling was obtained by ATAC sequencing and RNA sequencing. All data were obtained using embryo heads at 48 hpf.,,,,RNA seq of wild type sibling sample3,SAMD00399013,,sample name:rna rw337 48hpf WT rep 3|biological replicate:3,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00399013,DRX303511,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008373,Illumina NovaSeq 6000 paired end sequencing of SAMD00399013,,,,23076492885.0,76679376.0,DRR314108,0:150.51 1:150.44,A:6146969533;C:5373690527;G:5458576301;T:6095818756;N:1437768,150,150,,,6146969533,5373690527,5458576301,6095818756,1437768,DRX303511,DRS233566,DRA012572,OIST|Developmental Neurobiology Unit,Okinawa Institute of Science and Technology,2,0.94223,0.94614,0.10721,0.10288,0.68745,0.68621,0.4728,0.47157,151,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2022-04-01,Hatching,Embryo,Undetermined,Embryo Imprecise 1,DRR314107,DRX303510,DRS233565,DRP008373,PRJDB12134,Comparison of expression profile between banp mutant and wildtype sibling.,DRP008373,Other,To characterize the physiological function of Banp the expression profile of banp mutant and wild type sibling was obtained by ATAC sequencing and RNA sequencing. All data were obtained using embryo heads at 48 hpf.,,,,RNA seq of wild type sibling sample2,SAMD00399012,,sample name:rna rw337 48hpf WT rep 2|biological replicate:2,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00399012,DRX303510,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008373,Illumina NovaSeq 6000 paired end sequencing of SAMD00399012,,,,26091623771.0,86694066.0,DRR314107,0:150.51 1:150.45,A:6955374552;C:6050013285;G:6169385096;T:6915281392;N:1569446,150,150,,,6955374552,6050013285,6169385096,6915281392,1569446,DRX303510,DRS233565,DRA012572,OIST|Developmental Neurobiology Unit,Okinawa Institute of Science and Technology,2,0.94028,0.94277,0.11038,0.10462,0.68288,0.68134,0.46992,0.47227,150,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2022-04-01,Hatching,Embryo,Undetermined,Embryo Imprecise 2,DRR314106,DRX303509,DRS233564,DRP008373,PRJDB12134,Comparison of expression profile between banp mutant and wildtype sibling.,DRP008373,Other,To characterize the physiological function of Banp the expression profile of banp mutant and wild type sibling was obtained by ATAC sequencing and RNA sequencing. All data were obtained using embryo heads at 48 hpf.,,,,RNA seq of wild type sibling sample1,SAMD00399011,,sample name:rna rw337 48hpf WT rep 1|biological replicate:1,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00399011,DRX303509,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008373,Illumina NovaSeq 6000 paired end sequencing of SAMD00399011,,,,23833525756.0,79191795.0,DRR314106,0:150.51 1:150.44,A:6324521565;C:5556755459;G:5694025045;T:6256748423;N:1475264,150,150,,,6324521565,5556755459,5694025045,6256748423,1475264,DRX303509,DRS233564,DRA012572,OIST|Developmental Neurobiology Unit,Okinawa Institute of Science and Technology,2,0.94723,0.94975,0.09521,0.09114,0.6776,0.67819,0.46045,0.46153,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2022-04-01,Hatching,Embryo,Undetermined,Embryo Imprecise 3,DRR314105,DRX303508,DRS233563,DRP008373,PRJDB12134,Comparison of expression profile between banp mutant and wildtype sibling.,DRP008373,Other,To characterize the physiological function of Banp the expression profile of banp mutant and wild type sibling was obtained by ATAC sequencing and RNA sequencing. All data were obtained using embryo heads at 48 hpf.,,,,RNA seq of banp mutant sample3,SAMD00399010,,sample name:rna rw337 48hpf Mutant rep 3|biological replicate:3,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00399010,DRX303508,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008373,Illumina NovaSeq 6000 paired end sequencing of SAMD00399010,,,,27688386114.0,92009317.0,DRR314105,0:150.49 1:150.44,A:7681451080;C:6071566134;G:6242782106;T:7690830655;N:1756139,150,150,,,7681451080,6071566134,6242782106,7690830655,1756139,DRX303508,DRS233563,DRA012572,OIST|Developmental Neurobiology Unit,Okinawa Institute of Science and Technology,2,0.91038,0.91556,0.17971,0.16967,0.67718,0.67716,0.47042,0.46425,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2022-04-01,Hatching,Embryo,Undetermined,Embryo Imprecise 4,DRR314104,DRX303507,DRS233562,DRP008373,PRJDB12134,Comparison of expression profile between banp mutant and wildtype sibling.,DRP008373,Other,To characterize the physiological function of Banp the expression profile of banp mutant and wild type sibling was obtained by ATAC sequencing and RNA sequencing. All data were obtained using embryo heads at 48 hpf.,,,,RNA seq of banp mutant sample2,SAMD00399009,,sample name:rna rw337 48hpf Mutant rep 2|biological replicate:2,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00399009,DRX303507,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008373,Illumina NovaSeq 6000 paired end sequencing of SAMD00399009,,,,22970994572.0,76322352.0,DRR314104,0:150.52 1:150.46,A:6142535654;C:5310453740;G:5430124468;T:6086516676;N:1364034,150,150,,,6142535654,5310453740,5430124468,6086516676,1364034,DRX303507,DRS233562,DRA012572,OIST|Developmental Neurobiology Unit,Okinawa Institute of Science and Technology,2,0.93707,0.94063,0.12175,0.11613,0.67825,0.67649,0.46485,0.46905,147,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2022-04-01,Hatching,Embryo,Undetermined,Embryo Imprecise 5,DRR314103,DRX303506,DRS233561,DRP008373,PRJDB12134,Comparison of expression profile between banp mutant and wildtype sibling.,DRP008373,Other,To characterize the physiological function of Banp the expression profile of banp mutant and wild type sibling was obtained by ATAC sequencing and RNA sequencing. All data were obtained using embryo heads at 48 hpf.,,,,RNA seq of banp mutant sample1,SAMD00399008,,sample name:rna rw337 48hpf Mutant rep 1|biological replicate:1,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00399008,DRX303506,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008373,Illumina NovaSeq 6000 paired end sequencing of SAMD00399008,,,,23637901630.0,78541449.0,DRR314103,0:150.51 1:150.45,A:6359178134;C:5420730443;G:5530700546;T:6325864263;N:1428244,150,150,,,6359178134,5420730443,5530700546,6325864263,1428244,DRX303506,DRS233561,DRA012572,OIST|Developmental Neurobiology Unit,Okinawa Institute of Science and Technology,2,0.9308,0.93545,0.13172,0.12462,0.68219,0.6814,0.46842,0.46984,150,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2022-04-01,Hatching,Embryo,Undetermined,Embryo Imprecise 6,DRR315802,DRX305194,DRS231989,DRP008318,PRJDB12206,Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes,DRP008318,Transcriptome Analysis,"Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant ""rw147"" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis.",,,,wildtype sibling sample4,SAMD00400823,,sample name:rw147 2.5dpf wildtype rep 4|biological replicate:4,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00400823,DRX305194,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008318,Illumina NovaSeq 6000 paired end sequencing of SAMD00400823,,,,10262353995.0,34151547.0,DRR315802,0:150.27 1:150.22,A:2735078560;C:2386126821;G:2433638250;T:2707202814;N:307550,150,150,,,2735078560,2386126821,2433638250,2707202814,307550,DRX305194,DRS231989,DRA012640,OIST|Developmental Neurobiology Unit,Developmental Neurobiology Unit,2,0.95231,0.95295,0.09229,0.08773,0.71819,0.72107,0.46746,0.46617,151,150,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Unknown,2022-03-16,Hatching,Embryo,Undetermined,Embryo Imprecise 7,DRR315801,DRX305193,DRS231988,DRP008318,PRJDB12206,Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes,DRP008318,Transcriptome Analysis,"Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant ""rw147"" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis.",,,,wildtype sibling sample3,SAMD00400822,,sample name:rw147 2.5dpf wildtype rep 3|biological replicate:3,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00400822,DRX305193,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008318,Illumina NovaSeq 6000 paired end sequencing of SAMD00400822,,,,11516368634.0,38355888.0,DRR315801,0:150.15 1:150.10,A:3080341643;C:2678048339;G:2713051368;T:3044449330;N:477954,150,150,,,3080341643,2678048339,2713051368,3044449330,477954,DRX305193,DRS231988,DRA012640,OIST|Developmental Neurobiology Unit,Developmental Neurobiology Unit,2,0.95353,0.95634,0.08909,0.08533,0.71374,0.71252,0.45986,0.46059,150,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Unknown,2022-03-16,Hatching,Embryo,Undetermined,Embryo Imprecise 8,DRR315800,DRX305192,DRS231987,DRP008318,PRJDB12206,Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes,DRP008318,Transcriptome Analysis,"Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant ""rw147"" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis.",,,,wildtype sibling sample2,SAMD00400821,,sample name:rw147 2.5dpf wildtype rep 2|biological replicate:2,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00400821,DRX305192,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008318,Illumina NovaSeq 6000 paired end sequencing of SAMD00400821,,,,8814057148.0,29367513.0,DRR315800,0:150.09 1:150.04,A:2350403211;C:2054073465;G:2083044327;T:2326181188;N:354957,150,150,,,2350403211,2054073465,2083044327,2326181188,354957,DRX305192,DRS231987,DRA012640,OIST|Developmental Neurobiology Unit,Developmental Neurobiology Unit,2,0.95287,0.95643,0.0891,0.08586,0.70309,0.70252,0.46384,0.46281,151,149,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Unknown,2022-03-16,Hatching,Embryo,Undetermined,Embryo Imprecise 9,DRR315799,DRX305191,DRS231986,DRP008318,PRJDB12206,Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes,DRP008318,Transcriptome Analysis,"Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant ""rw147"" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis.",,,,wildtype sibling sample1,SAMD00400820,,sample name:rw147 2.5dpf wildtype rep 1|biological replicate:1,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00400820,DRX305191,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008318,Illumina NovaSeq 6000 paired end sequencing of SAMD00400820,,,,10491955578.0,34900682.0,DRR315799,0:150.34 1:150.28,A:2796521111;C:2446218287;G:2483414564;T:2765477785;N:323831,150,150,,,2796521111,2446218287,2483414564,2765477785,323831,DRX305191,DRS231986,DRA012640,OIST|Developmental Neurobiology Unit,Developmental Neurobiology Unit,2,0.9539,0.95646,0.08185,0.07808,0.70025,0.70013,0.44713,0.44987,150,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Unknown,2022-03-16,Hatching,Embryo,Undetermined,Embryo Imprecise 10,DRR315798,DRX305190,DRS231985,DRP008318,PRJDB12206,Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes,DRP008318,Transcriptome Analysis,"Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant ""rw147"" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis.",,,,strip1 mutant sample4,SAMD00400819,,sample name:rw147 2.5dpf Mutant rep 4|biological replicate:4,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00400819,DRX305190,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008318,Illumina NovaSeq 6000 paired end sequencing of SAMD00400819,,,,9197802250.0,30604326.0,DRR315798,0:150.30 1:150.24,A:2468967963;C:2130949980;G:2158692262;T:2438931017;N:261028,150,150,,,2468967963,2130949980,2158692262,2438931017,261028,DRX305190,DRS231985,DRA012640,OIST|Developmental Neurobiology Unit,Developmental Neurobiology Unit,2,0.95159,0.95439,0.10146,0.09758,0.71995,0.71983,0.46519,0.46797,150,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Unknown,2022-03-16,Hatching,Embryo,Undetermined,Embryo Imprecise 11,DRR315797,DRX305189,DRS231984,DRP008318,PRJDB12206,Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes,DRP008318,Transcriptome Analysis,"Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant ""rw147"" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis.",,,,strip1 mutant sample3,SAMD00400818,,sample name:rw147 2.5dpf Mutant rep 3|biological replicate:3,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00400818,DRX305189,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008318,Illumina NovaSeq 6000 paired end sequencing of SAMD00400818,,,,10498982078.0,34931731.0,DRR315797,0:150.31 1:150.25,A:2804535103;C:2445295179;G:2478768789;T:2770066062;N:316945,150,150,,,2804535103,2445295179,2478768789,2770066062,316945,DRX305189,DRS231984,DRA012640,OIST|Developmental Neurobiology Unit,Developmental Neurobiology Unit,2,0.95448,0.95652,0.0939,0.0887,0.71796,0.71847,0.46335,0.46615,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Unknown,2022-03-16,Hatching,Embryo,Undetermined,Embryo Imprecise 12,DRR315796,DRX305188,DRS231983,DRP008318,PRJDB12206,Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes,DRP008318,Transcriptome Analysis,"Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant ""rw147"" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis.",,,,strip1 mutant sample2,SAMD00400817,,sample name:rw147 2.5dpf Mutant rep 2|biological replicate:2,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00400817,DRX305188,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008318,Illumina NovaSeq 6000 paired end sequencing of SAMD00400817,,,,9850145990.0,32782079.0,DRR315796,0:150.26 1:150.21,A:2636205537;C:2286508705;G:2319481100;T:2607600624;N:350024,150,150,,,2636205537,2286508705,2319481100,2607600624,350024,DRX305188,DRS231983,DRA012640,OIST|Developmental Neurobiology Unit,Developmental Neurobiology Unit,2,0.95193,0.95472,0.09722,0.09375,0.7138,0.71299,0.45542,0.45994,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Unknown,2022-03-16,Hatching,Embryo,Undetermined,Embryo Imprecise 13,DRR315795,DRX305187,DRS231982,DRP008318,PRJDB12206,Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes,DRP008318,Transcriptome Analysis,"Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant ""rw147"" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis.",,,,strip1 mutant sample1,SAMD00400816,,sample name:rw147 2.5dpf Mutant rep 1|biological replicate:1,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing of SAMD00400816,DRX305187,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,1510Application ReadForward11Application ReadReverse76,DRP008318,Illumina NovaSeq 6000 paired end sequencing of SAMD00400816,,,,9542039835.0,31780260.0,DRR315795,0:150.15 1:150.10,A:2543384204;C:2224374632;G:2258183435;T:2515655339;N:442225,150,150,,,2543384204,2224374632,2258183435,2515655339,442225,DRX305187,DRS231982,DRA012640,OIST|Developmental Neurobiology Unit,Developmental Neurobiology Unit,2,0.9528,0.95591,0.08656,0.0828,0.70352,0.70331,0.45316,0.44914,151,151,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,unknown,bulk,bulk,bulk,,Unknown,2022-03-16,Hatching,Embryo,Undetermined,Embryo Imprecise 9337,ERR2865439,ERX2871399,ERS2871019,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development we identified prominent coloboma and cell autonomous retinal neuron differentiation lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when and locations where post mitotic cells have differentiated in wild type siblings. Indeed RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated for instance in axon guidance that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise or promote transitions in cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,sibling 3,SAMEA5059848,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059848|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele sibling3|common name:zebrafish|sample name:ele sibling3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 6,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_sib_F_CTTGTA_L004_R2_001.fastq.gz ele_sib_F_CTTGTA_L004_R1_001.fastq.gz,fastq fastq,2823621200.0,14118106.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 6,0:100 1:100,A:752781583;C:663838191;G:656422705;T:750213027;N:365694,100,100,,,752781583,663838191,656422705,750213027,365694,ERX2871399,ERS2871019,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.95595,0.95486,0.09407,0.09431,0.67529,0.67673,0.45173,0.44515,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise 9338,ERR2865438,ERX2871398,ERS2871018,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development we identified prominent coloboma and cell autonomous retinal neuron differentiation lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when and locations where post mitotic cells have differentiated in wild type siblings. Indeed RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated for instance in axon guidance that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise or promote transitions in cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,sibling 2,SAMEA5059847,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059847|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele sibling2|common name:zebrafish|sample name:ele sibling2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 5,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_sib_D_GCCAAT_L004_R1_001.fastq.gz ele_sib_D_GCCAAT_L004_R2_001.fastq.gz,fastq fastq,4217962600.0,21089813.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 5,0:100 1:100,A:1119324653;C:996982862;G:984906708;T:1116209552;N:538825,100,100,,,1119324653,996982862,984906708,1116209552,538825,ERX2871398,ERS2871018,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.95341,0.95274,0.09215,0.09238,0.67296,0.67493,0.46185,0.4648,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise 9339,ERR2865437,ERX2871397,ERS2871017,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development we identified prominent coloboma and cell autonomous retinal neuron differentiation lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when and locations where post mitotic cells have differentiated in wild type siblings. Indeed RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated for instance in axon guidance that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise or promote transitions in cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,sibling 1,SAMEA5059846,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059846|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele sibling1|common name:zebrafish|sample name:ele sibling1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 4,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_sib_B_TGACCA_L004_R1_001.fastq.gz ele_sib_B_TGACCA_L004_R2_001.fastq.gz,fastq fastq,5241628000.0,26208140.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 4,0:100 1:100,A:1393802799;C:1235804455;G:1219328189;T:1392021956;N:670601,100,100,,,1393802799,1235804455,1219328189,1392021956,670601,ERX2871397,ERS2871017,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.95296,0.95133,0.10275,0.1028,0.67018,0.67146,0.46488,0.46482,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise 9340,ERR2865436,ERX2871396,ERS2871016,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development we identified prominent coloboma and cell autonomous retinal neuron differentiation lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when and locations where post mitotic cells have differentiated in wild type siblings. Indeed RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated for instance in axon guidance that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise or promote transitions in cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,mutant3,SAMEA5059845,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059845|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele mutant3|common name:zebrafish|sample name:ele mutant3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 3,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_E_CAGATC_L004_R1_001.fastq.gz ele_E_CAGATC_L004_R2_001.fastq.gz,fastq fastq,3529752000.0,17648760.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 3,0:100 1:100,A:933354224;C:837396695;G:828677816;T:929860782;N:462483,100,100,,,933354224,837396695,828677816,929860782,462483,ERX2871396,ERS2871016,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.95621,0.95302,0.08584,0.08536,0.67048,0.67146,0.46615,0.46682,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise 9341,ERR2865435,ERX2871395,ERS2871015,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development we identified prominent coloboma and cell autonomous retinal neuron differentiation lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when and locations where post mitotic cells have differentiated in wild type siblings. Indeed RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated for instance in axon guidance that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise or promote transitions in cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,mutant2,SAMEA5059844,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059844|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele mutant2|common name:zebrafish|sample name:ele mutant2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 2,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_C_ACAGTG_L004_R1_001.fastq.gz ele_C_ACAGTG_L004_R2_001.fastq.gz,fastq fastq,3119723800.0,15598619.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 2,0:100 1:100,A:828335602;C:736691663;G:727853394;T:826449133;N:394008,100,100,,,828335602,736691663,727853394,826449133,394008,ERX2871395,ERS2871015,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.94967,0.94864,0.0992,0.09955,0.65928,0.66014,0.47042,0.46835,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise 9342,ERR2865434,ERX2871394,ERS2871014,ERP111778,PRJEB29472,RNAseq analysis of slbp mutants in Zebrafish,ena-STUDY-Department of Cell and Developmental Biology-01-11-2018-15:10:58:144-14,Other,Through forward genetic screening for mutations affecting visual system development we identified prominent coloboma and cell autonomous retinal neuron differentiation lamination and retinal axon projection defects in eisspalte ele mutant zebrafish. Additional axonal deficits were present most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp which encodes a conserved RNA stem loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when and locations where post mitotic cells have differentiated in wild type siblings. Indeed RNAseq analysis showed down regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated for instance in axon guidance that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise or promote transitions in cell state and require the correct function of Slbp dependent histone and chromatin regulatory genes.,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 01,,,mutant1,SAMEA5059843,Department of Cell and Developmental Biology,ENA FIRST PUBLIC:2018 11 02T17:01:55Z|ENA LAST UPDATE:2018 11 01T15:11:02Z|External Id:SAMEA5059843|INSDC center name:Department of Cell and Developmental Biology|INSDC first public:2018 11 02T17:01:55Z|INSDC last update:2018 11 01T15:11:02Z|INSDC status:public|Submitter Id:ele mutant1|common name:zebrafish|sample name:ele mutant1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq 3000 paired end sequencing,ena EXPERIMENT Department of Cell and Developmental Biology 01 11 2018 15:10:57:716 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,other,PAIRED,ILLUMINA,Illumina HiSeq 3000,,ERP111778,Illumina HiSeq 3000 paired end sequencing,ENA FIRST PUBLIC:2018 11 02|ENA LAST UPDATE:2018 11 16,ele_A_CGATGT_L004_R1_001.fastq.gz ele_A_CGATGT_L004_R2_001.fastq.gz,fastq fastq,2181939600.0,10909698.0,ena RUN Department of Cell and Developmental Biology 01 11 2018 15:10:57:717 1,0:100 1:100,A:578028200;C:516249107;G:510965740;T:576417363;N:279190,100,100,,,578028200,516249107,510965740,576417363,279190,ERX2871394,ERS2871014,ERA1643817,Department of Cell and Developmental Biology|European Nucleotide Archive,Department of Cell and Developmental Biology,2,0.94968,0.94939,0.1131,0.11293,0.66245,0.66251,0.46654,0.47475,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,other,unknown,bulk,unknown,unknown,,Unknown,2018-11-01,Undetermined,Embryo,Undetermined,Embryo Imprecise 14875,ERR12071836,ERX11454461,ERS16387703,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 1,SAMEA114399011,University of East Anglia,ENA FIRST PUBLIC:2023 10 06T08:31:44Z|ENA LAST UPDATE:2023 10 06T08:31:44Z|External Id:SAMEA114399011|INSDC center name:University of East Anglia|INSDC first public:2023 10 06T08:31:44Z|INSDC last update:2023 10 06T08:31:44Z|INSDC status:public|Submitter Id:115 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66715,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 10 06|ENA LAST UPDATE:2023 10 06,115-1_S69_L006_R1_001.fastq.gz 115-1_S69_L006_R2_001.fastq.gz,fastq fastq,12813280704.0,50846352.0,ena RUN TAB 21 09 2023 13:32:15:295 66716,0:126 1:126,A:2451295493;C:3891843753;G:4001866488;T:2448421880;N:19853090,126,126,,,2451295493,3891843753,4001866488,2448421880,19853090,ERX11454461,ERS16387703,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89907,0.8514,0.17845,0.173,0.80369,0.81085,0.83252,0.83449,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14876,ERR12071855,ERX11454480,ERS16387722,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 5,SAMEA114399030,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:300 66753,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-5_S40_L008_R1_001.fastq.gz 83-5_S40_L008_R2_001.fastq.gz,fastq fastq,13683493656.0,54299578.0,ena RUN TAB 21 09 2023 13:32:15:300 66754,0:126 1:126,A:2772843483;C:4073415925;G:4192640412;T:2641952920;N:2640916,126,126,,,2772843483,4073415925,4192640412,2641952920,2640916,ERX11454480,ERS16387722,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92452,0.92427,0.15846,0.16251,0.7777,0.77666,0.78362,0.77515,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14877,ERR12071854,ERX11454479,ERS16387721,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 4,SAMEA114399029,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66751,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-4_S8_L002_R1_001.fastq.gz 83-4_S8_L002_R2_001.fastq.gz,fastq fastq,10045714644.0,39863947.0,ena RUN TAB 21 09 2023 13:32:15:300 66752,0:126 1:126,A:2037388456;C:2963530056;G:3069663461;T:1972981871;N:2150800,126,126,,,2037388456,2963530056,3069663461,1972981871,2150800,ERX11454479,ERS16387721,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91872,0.91762,0.16049,0.16307,0.76516,0.76644,0.77023,0.77199,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14878,ERR12071853,ERX11454478,ERS16387720,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 3,SAMEA114399028,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66749,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-3_S12_L003_R1_001.fastq.gz 83-3_S12_L003_R2_001.fastq.gz,fastq fastq,17333567748.0,68783999.0,ena RUN TAB 21 09 2023 13:32:15:299 66750,0:126 1:126,A:3399006637;C:5245167754;G:5416988818;T:3269621086;N:2783453,126,126,,,3399006637,5245167754,5416988818,3269621086,2783453,ERX11454478,ERS16387720,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93044,0.93099,0.14459,0.1479,0.77287,0.774,0.78248,0.78348,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14879,ERR12071852,ERX11454477,ERS16387719,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E83 1,SAMEA114399027,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:83 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:83 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66747,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,83-1_S4_L001_R1_001.fastq.gz 83-1_S4_L001_R2_001.fastq.gz,fastq fastq,14977164132.0,59433191.0,ena RUN TAB 21 09 2023 13:32:15:299 66748,0:126 1:126,A:3115980365;C:4357431505;G:4531888641;T:2968758582;N:3105039,126,126,,,3115980365,4357431505,4531888641,2968758582,3105039,ERX11454477,ERS16387719,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89622,0.89549,0.15698,0.16074,0.76558,0.76779,0.70301,0.73129,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14880,ERR12071851,ERX11454476,ERS16387718,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 5,SAMEA114399026,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:299 66745,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-5_S3_L001_R1_001.fastq.gz 82-5_S3_L001_R2_001.fastq.gz,fastq fastq,14575744764.0,57840257.0,ena RUN TAB 21 09 2023 13:32:15:299 66746,0:126 1:126,A:3079286238;C:4161280385;G:4315514217;T:3016549159;N:3114765,126,126,,,3079286238,4161280385,4315514217,3016549159,3114765,ERX11454476,ERS16387718,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.82553,0.82489,0.15941,0.16124,0.77337,0.77518,0.73773,0.75718,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14881,ERR12071850,ERX11454475,ERS16387717,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 3,SAMEA114399025,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66743,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-3_S11_L003_R1_001.fastq.gz 82-3_S11_L003_R2_001.fastq.gz,fastq fastq,15753924144.0,62515572.0,ena RUN TAB 21 09 2023 13:32:15:299 66744,0:126 1:126,A:3294606329;C:4538635184;G:4708756438;T:3209425450;N:2500743,126,126,,,3294606329,4538635184,4708756438,3209425450,2500743,ERX11454475,ERS16387717,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.8642,0.86388,0.1596,0.16298,0.77051,0.77297,0.7599,0.7352,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14882,ERR12071849,ERX11454474,ERS16387716,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 2,SAMEA114399024,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66741,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 22,82-2_S7_L002_R1_001.fastq.gz 82-2_S7_L002_R2_001.fastq.gz,fastq fastq,20680686684.0,82066217.0,ena RUN TAB 21 09 2023 13:32:15:298 66742,0:126 1:126,A:4358995918;C:5919331822;G:6101024757;T:4296885228;N:4448959,126,126,,,4358995918,5919331822,6101024757,4296885228,4448959,ERX11454474,ERS16387716,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.88722,0.88591,0.1721,0.1755,0.76207,0.76394,0.74661,0.7483,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14883,ERR12071848,ERX11454473,ERS16387715,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E82 1,SAMEA114399023,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:82 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:82 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66739,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,82-1_S39_L008_R1_001.fastq.gz 82-1_S39_L008_R2_001.fastq.gz,fastq fastq,19062862812.0,75646281.0,ena RUN TAB 21 09 2023 13:32:15:298 66740,0:126 1:126,A:4015412618;C:5485657253;G:5643544062;T:3914646370;N:3602509,126,126,,,4015412618,5485657253,5643544062,3914646370,3602509,ERX11454473,ERS16387715,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.82331,0.82289,0.14959,0.15262,0.77786,0.77368,0.75014,0.71846,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14884,ERR12071847,ERX11454472,ERS16387714,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 5,SAMEA114399022,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:298 66737,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-5_S6_L002_R1_001.fastq.gz 81-5_S6_L002_R2_001.fastq.gz,fastq fastq,14105505960.0,55974230.0,ena RUN TAB 21 09 2023 13:32:15:298 66738,0:126 1:126,A:2867593807;C:4154280778;G:4295901883;T:2784713422;N:3016070,126,126,,,2867593807,4154280778,4295901883,2784713422,3016070,ERX11454472,ERS16387714,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89055,0.89059,0.15225,0.15462,0.77031,0.7709,0.77204,0.74565,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14885,ERR12071846,ERX11454471,ERS16387713,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 4,SAMEA114399021,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66735,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-4_S38_L008_R1_001.fastq.gz 81-4_S38_L008_R2_001.fastq.gz,fastq fastq,14056344540.0,55779145.0,ena RUN TAB 21 09 2023 13:32:15:298 66736,0:126 1:126,A:2941182079;C:4065026103;G:4178339299;T:2869101906;N:2695153,126,126,,,2941182079,4065026103,4178339299,2869101906,2695153,ERX11454471,ERS16387713,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.85089,0.84987,0.16263,0.16502,0.77837,0.77283,0.74931,0.72487,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14886,ERR12071845,ERX11454470,ERS16387712,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 2,SAMEA114399020,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66733,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-2_S10_L003_R2_001.fastq.gz 81-2_S10_L003_R1_001.fastq.gz,fastq fastq,12344567004.0,48986377.0,ena RUN TAB 21 09 2023 13:32:15:297 66734,0:126 1:126,A:2564870489;C:3577715066;G:3699995887;T:2500054422;N:1931140,126,126,,,2564870489,3577715066,3699995887,2500054422,1931140,ERX11454470,ERS16387712,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.81332,0.81278,0.14345,0.14595,0.77962,0.78011,0.77642,0.76714,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14887,ERR12071844,ERX11454469,ERS16387711,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E81 1,SAMEA114399019,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:81 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:81 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66731,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,81-1_S2_L001_R1_001.fastq.gz 81-1_S2_L001_R2_001.fastq.gz,fastq fastq,16863847056.0,66920028.0,ena RUN TAB 21 09 2023 13:32:15:297 66732,0:126 1:126,A:3484102096;C:4908704805;G:5083802657;T:3383595635;N:3641863,126,126,,,3484102096,4908704805,5083802657,3383595635,3641863,ERX11454469,ERS16387711,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90019,0.89959,0.16463,0.16683,0.76607,0.76792,0.76365,0.73132,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14888,ERR12071843,ERX11454468,ERS16387710,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 4,SAMEA114399018,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:297 66729,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-4_S9_L003_R1_001.fastq.gz 80-4_S9_L003_R2_001.fastq.gz,fastq fastq,22230539604.0,88216427.0,ena RUN TAB 21 09 2023 13:32:15:297 66730,0:126 1:126,A:4399600032;C:6651638899;G:6855034908;T:4320719496;N:3546269,126,126,,,4399600032,6651638899,6855034908,4320719496,3546269,ERX11454468,ERS16387710,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84585,0.84448,0.14327,0.14586,0.79078,0.79251,0.79803,0.79459,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14889,ERR12071842,ERX11454467,ERS16387709,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 3,SAMEA114399017,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66727,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-3_S1_L001_R2_001.fastq.gz 80-3_S1_L001_R1_001.fastq.gz,fastq fastq,17944230024.0,71207262.0,ena RUN TAB 21 09 2023 13:32:15:296 66728,0:126 1:126,A:3713490828;C:5207225554;G:5378396527;T:3641393155;N:3723960,126,126,,,3713490828,5207225554,5378396527,3641393155,3723960,ERX11454467,ERS16387709,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84454,0.84386,0.14922,0.15129,0.77264,0.77339,0.75797,0.76206,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14890,ERR12071841,ERX11454466,ERS16387708,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 2,SAMEA114399016,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66725,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-2_S5_L002_R1_001.fastq.gz 80-2_S5_L002_R2_001.fastq.gz,fastq fastq,16641238320.0,66036660.0,ena RUN TAB 21 09 2023 13:32:15:296 66726,0:126 1:126,A:3508280857;C:4757668643;G:4898009962;T:3473768461;N:3510397,126,126,,,3508280857,4757668643,4898009962,3473768461,3510397,ERX11454466,ERS16387708,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.86106,0.86111,0.16002,0.16307,0.7653,0.76542,0.72623,0.70444,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14891,ERR12071840,ERX11454465,ERS16387707,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E80 1,SAMEA114399015,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:80 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:80 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66723,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,80-1_S37_L008_R1_001.fastq.gz 80-1_S37_L008_R2_001.fastq.gz,fastq fastq,15269215248.0,60592124.0,ena RUN TAB 21 09 2023 13:32:15:296 66724,0:126 1:126,A:3161128702;C:4439474080;G:4571245477;T:3094451760;N:2915229,126,126,,,3161128702,4439474080,4571245477,3094451760,2915229,ERX11454465,ERS16387707,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.84008,0.83924,0.16203,0.16482,0.78486,0.78301,0.76847,0.76451,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14892,ERR12071839,ERX11454464,ERS16387706,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 5,SAMEA114399014,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:296 66721,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-5_S28_L005_R2_001.fastq.gz 115-5_S28_L005_R1_001.fastq.gz,fastq fastq,13704997824.0,54384912.0,ena RUN TAB 21 09 2023 13:32:15:296 66722,0:126 1:126,A:2791048995;C:4040751875;G:4161655954;T:2708992373;N:2548627,126,126,,,2791048995,4040751875,4161655954,2708992373,2548627,ERX11454464,ERS16387706,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92152,0.922,0.16432,0.16746,0.77977,0.77723,0.7658,0.74319,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14893,ERR12071838,ERX11454463,ERS16387705,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 4,SAMEA114399013,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66719,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-4_S73_L007_R1_001.fastq.gz 115-4_S73_L007_R2_001.fastq.gz,fastq fastq,13555374840.0,53791170.0,ena RUN TAB 21 09 2023 13:32:15:295 66720,0:126 1:126,A:2613879041;C:4131073531;G:4251239682;T:2536011431;N:23171155,126,126,,,2613879041,4131073531,4251239682,2536011431,23171155,ERX11454463,ERS16387705,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93971,0.93992,0.15435,0.15669,0.78766,0.79076,0.76218,0.78676,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14894,ERR12071837,ERX11454462,ERS16387704,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E115 2,SAMEA114399012,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:115 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:115 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:295 66717,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,115-2_S77_L008_R1_001.fastq.gz 115-2_S77_L008_R2_001.fastq.gz,fastq fastq,19309168872.0,76623686.0,ena RUN TAB 21 09 2023 13:32:15:295 66718,0:126 1:126,A:3659403129;C:5914313547;G:6102556630;T:3596021103;N:36874463,126,126,,,3659403129,5914313547,6102556630,3596021103,36874463,ERX11454462,ERS16387704,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90206,0.90085,0.164,0.16686,0.81477,0.81856,0.84674,0.83786,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14895,ERR12071835,ERX11454460,ERS16387702,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 5,SAMEA114399010,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66713,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-5_S68_L006_R1_001.fastq.gz 114-5_S68_L006_R2_001.fastq.gz,fastq fastq,14161249872.0,56195436.0,ena RUN TAB 21 09 2023 13:32:15:295 66714,0:126 1:126,A:2927386834;C:4098238005;G:4213138034;T:2900333935;N:22153064,126,126,,,2927386834,4098238005,4213138034,2900333935,22153064,ERX11454460,ERS16387702,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92035,0.91983,0.16785,0.17009,0.75864,0.76292,0.73081,0.75429,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14896,ERR12071834,ERX11454459,ERS16387701,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 4,SAMEA114399009,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66711,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-4_S76_L008_R1_001.fastq.gz 114-4_S76_L008_R2_001.fastq.gz,fastq fastq,12939456600.0,51347050.0,ena RUN TAB 21 09 2023 13:32:15:294 66712,0:126 1:126,A:2686900916;C:3725092053;G:3826228928;T:2676529407;N:24705296,126,126,,,2686900916,3725092053,3826228928,2676529407,24705296,ERX11454459,ERS16387701,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9171,0.91541,0.19718,0.20005,0.76759,0.77285,0.75547,0.75357,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14897,ERR12071833,ERX11454458,ERS16387700,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 3,SAMEA114399008,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66709,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-3_S72_L007_R1_001.fastq.gz 114-3_S72_L007_R2_001.fastq.gz,fastq fastq,15510255012.0,61548631.0,ena RUN TAB 21 09 2023 13:32:15:294 66710,0:126 1:126,A:3024458012;C:4641826332;G:4764527525;T:3052903565;N:26539578,126,126,,,3024458012,4641826332,4764527525,3052903565,26539578,ERX11454458,ERS16387700,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.85112,0.84868,0.17054,0.17432,0.79547,0.79851,0.73756,0.8091,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14898,ERR12071832,ERX11454457,ERS16387699,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E114 1,SAMEA114399007,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:114 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:114 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:294 66707,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,114-1_S27_L005_R1_001.fastq.gz 114-1_S27_L005_R2_001.fastq.gz,fastq fastq,15544755576.0,61685538.0,ena RUN TAB 21 09 2023 13:32:15:294 66708,0:126 1:126,A:3077913021;C:4638218386;G:4760672592;T:3065112184;N:2839393,126,126,,,3077913021,4638218386,4760672592,3065112184,2839393,ERX11454457,ERS16387699,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90822,0.90777,0.1775,0.18112,0.79263,0.78808,0.82214,0.79489,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14899,ERR12071831,ERX11454456,ERS16387698,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 9,SAMEA114399006,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 9|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 9|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66705,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-9_S67_L006_R1_001.fastq.gz 113-9_S67_L006_R2_001.fastq.gz,fastq fastq,15414133644.0,61167197.0,ena RUN TAB 21 09 2023 13:32:15:293 66706,0:126 1:126,A:3185120611;C:4467167719;G:4606569127;T:3131094561;N:24181626,126,126,,,3185120611,4467167719,4606569127,3131094561,24181626,ERX11454456,ERS16387698,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94176,0.94197,0.17489,0.17743,0.76063,0.76295,0.75218,0.75546,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14900,ERR12071830,ERX11454455,ERS16387697,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 13,SAMEA114399005,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 13|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 13|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66703,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-13_S71_L007_R1_001.fastq.gz 113-13_S71_L007_R2_001.fastq.gz,fastq fastq,18125498160.0,71926580.0,ena RUN TAB 21 09 2023 13:32:15:293 66704,0:126 1:126,A:3681246224;C:5316613237;G:5486708206;T:3609735923;N:31194570,126,126,,,3681246224,5316613237,5486708206,3609735923,31194570,ERX11454455,ERS16387697,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93952,0.93814,0.1717,0.17395,0.76627,0.76976,0.76533,0.72702,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14901,ERR12071829,ERX11454454,ERS16387696,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 11,SAMEA114399004,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 11|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 11|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66701,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-11_S26_L005_R1_001.fastq.gz 113-11_S26_L005_R2_001.fastq.gz,fastq fastq,17918758872.0,71106186.0,ena RUN TAB 21 09 2023 13:32:15:293 66702,0:126 1:126,A:3460235614;C:5469489967;G:5622549540;T:3363214846;N:3268905,126,126,,,3460235614,5469489967,5622549540,3363214846,3268905,ERX11454454,ERS16387696,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94836,0.94773,0.16512,0.16708,0.79843,0.79251,0.79716,0.79211,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14902,ERR12071828,ERX11454453,ERS16387695,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E113 10,SAMEA114399003,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:113 10|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:113 10|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:293 66699,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,113-10_S75_L008_R1_001.fastq.gz 113-10_S75_L008_R2_001.fastq.gz,fastq fastq,22137738588.0,87848169.0,ena RUN TAB 21 09 2023 13:32:15:293 66700,0:126 1:126,A:4240961737;C:6714114283;G:6924397580;T:4216314997;N:41949991,126,126,,,4240961737,6714114283,6924397580,4216314997,41949991,ERX11454453,ERS16387695,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94101,0.93949,0.15969,0.16256,0.782,0.78429,0.78877,0.78359,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14903,ERR12071827,ERX11454452,ERS16387694,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 8,SAMEA114399002,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 8|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 8|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66697,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-8_S74_L008_R1_001.fastq.gz 112-8_S74_L008_R2_001.fastq.gz,fastq fastq,15579825912.0,61824706.0,ena RUN TAB 21 09 2023 13:32:15:292 66698,0:126 1:126,A:3262384535;C:4470619467;G:4585527202;T:3231451715;N:29842993,126,126,,,3262384535,4470619467,4585527202,3231451715,29842993,ERX11454452,ERS16387694,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92603,0.92484,0.18249,0.18589,0.75917,0.76335,0.73196,0.72728,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14904,ERR12071826,ERX11454451,ERS16387693,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 7,SAMEA114399001,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 7|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 7|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66695,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-7_S66_L006_R1_001.fastq.gz 112-7_S66_L006_R2_001.fastq.gz,fastq fastq,20203529976.0,80172738.0,ena RUN TAB 21 09 2023 13:32:15:292 66696,0:126 1:126,A:4115948087;C:5902381830;G:6064166888;T:4089530757;N:31502414,126,126,,,4115948087,5902381830,6064166888,4089530757,31502414,ERX11454451,ERS16387693,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92896,0.92845,0.17083,0.17434,0.76146,0.76337,0.6945,0.74323,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14905,ERR12071825,ERX11454450,ERS16387692,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 5,SAMEA114399000,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 5|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 5|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66693,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-5_S70_L007_R1_001.fastq.gz 112-5_S70_L007_R2_001.fastq.gz,fastq fastq,14979433392.0,59442196.0,ena RUN TAB 21 09 2023 13:32:15:292 66694,0:126 1:126,A:2989976300;C:4427492967;G:4545641644;T:2990769657;N:25552824,126,126,,,2989976300,4427492967,4545641644,2990769657,25552824,ERX11454450,ERS16387692,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9139,0.91293,0.18796,0.1912,0.78443,0.78597,0.79005,0.77582,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14906,ERR12071824,ERX11454449,ERS16387691,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E112 4,SAMEA114398999,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:112 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:112 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:292 66691,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,112-4_S25_L005_R1_001.fastq.gz 112-4_S25_L005_R2_001.fastq.gz,fastq fastq,14850028368.0,58928684.0,ena RUN TAB 21 09 2023 13:32:15:292 66692,0:126 1:126,A:3108605429;C:4289376091;G:4392727411;T:3056545411;N:2774026,126,126,,,3108605429,4289376091,4392727411,3056545411,2774026,ERX11454449,ERS16387691,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92451,0.92555,0.17853,0.18265,0.76501,0.76106,0.73074,0.72504,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14907,ERR12071823,ERX11454448,ERS16387690,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 4,SAMEA114398998,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66689,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-4_S8_L002_R1_001.fastq.gz 111-4_S8_L002_R2_001.fastq.gz,fastq fastq,17826911676.0,70741713.0,ena RUN TAB 21 09 2023 13:32:15:291 66690,0:126 1:126,A:3435687537;C:5460019012;G:5625529849;T:3303228205;N:2447073,126,126,,,3435687537,5460019012,5625529849,3303228205,2447073,ERX11454448,ERS16387690,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95917,0.95936,0.18841,0.18992,0.81367,0.81523,0.79429,0.81623,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14908,ERR12071822,ERX11454447,ERS16387689,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 3,SAMEA114398997,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66687,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-3_S16_L004_R1_001.fastq.gz 111-3_S16_L004_R2_001.fastq.gz,fastq fastq,16893915192.0,67039346.0,ena RUN TAB 21 09 2023 13:32:15:291 66688,0:126 1:126,A:3264486030;C:5167828698;G:5330646003;T:3128597235;N:2357226,126,126,,,3264486030,5167828698,5330646003,3128597235,2357226,ERX11454447,ERS16387689,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96382,0.96339,0.19876,0.20112,0.81893,0.82102,0.81987,0.79966,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14909,ERR12071821,ERX11454446,ERS16387688,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 2,SAMEA114398996,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66685,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-2_S12_L003_R1_001.fastq.gz 111-2_S12_L003_R2_001.fastq.gz,fastq fastq,19135485684.0,75934467.0,ena RUN TAB 21 09 2023 13:32:15:291 66686,0:126 1:126,A:3609533802;C:5946063742;G:6161038930;T:3416257009;N:2592201,126,126,,,3609533802,5946063742,6161038930,3416257009,2592201,ERX11454446,ERS16387688,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96183,0.96186,0.17579,0.17791,0.81972,0.82047,0.77379,0.82377,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14910,ERR12071820,ERX11454445,ERS16387687,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E111 1,SAMEA114398995,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:111 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:111 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:291 66683,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,111-1_S4_L001_R1_001.fastq.gz 111-1_S4_L001_R2_001.fastq.gz,fastq fastq,21082514796.0,83660773.0,ena RUN TAB 21 09 2023 13:32:15:291 66684,0:126 1:126,A:4043020782;C:6480787651;G:6689150606;T:3865255761;N:4299996,126,126,,,4043020782,6480787651,6689150606,3865255761,4299996,ERX11454445,ERS16387687,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96289,0.963,0.18676,0.18828,0.81791,0.81988,0.78774,0.81813,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14911,ERR12071819,ERX11454444,ERS16387686,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 4,SAMEA114398994,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66681,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-4_S3_L001_R2_001.fastq.gz 110-4_S3_L001_R1_001.fastq.gz,fastq fastq,14687310204.0,58282977.0,ena RUN TAB 21 09 2023 13:32:15:290 66682,0:126 1:126,A:2926150199;C:4367282172;G:4510560380;T:2880182221;N:3135232,126,126,,,2926150199,4367282172,4510560380,2880182221,3135232,ERX11454444,ERS16387686,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93442,0.934,0.1706,0.17527,0.77611,0.7782,0.79075,0.77981,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14912,ERR12071818,ERX11454443,ERS16387685,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 3,SAMEA114398993,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66679,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-3_S11_L003_R2_001.fastq.gz 110-3_S11_L003_R1_001.fastq.gz,fastq fastq,13850712792.0,54963146.0,ena RUN TAB 21 09 2023 13:32:15:290 66680,0:126 1:126,A:2754372507;C:4139837776;G:4290528181;T:2664097645;N:1876683,126,126,,,2754372507,4139837776,4290528181,2664097645,1876683,ERX11454443,ERS16387685,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94187,0.94169,0.16695,0.17006,0.77824,0.78044,0.7861,0.7945,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14913,ERR12071817,ERX11454442,ERS16387684,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 2,SAMEA114398992,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66677,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-2_S15_L004_R2_001.fastq.gz 110-2_S15_L004_R1_001.fastq.gz,fastq fastq,15866607708.0,62962729.0,ena RUN TAB 21 09 2023 13:32:15:290 66678,0:126 1:126,A:3214891728;C:4688310800;G:4841314699;T:3119879854;N:2210627,126,126,,,3214891728,4688310800,4841314699,3119879854,2210627,ERX11454442,ERS16387684,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93647,0.93604,0.16699,0.17,0.76641,0.76909,0.76728,0.77117,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14914,ERR12071816,ERX11454441,ERS16387683,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E110 1,SAMEA114398991,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:110 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:110 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:290 66675,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,110-1_S7_L002_R2_001.fastq.gz 110-1_S7_L002_R1_001.fastq.gz,fastq fastq,15365002212.0,60972231.0,ena RUN TAB 21 09 2023 13:32:15:290 66676,0:126 1:126,A:3114598527;C:4531528684;G:4684815069;T:3031977480;N:2082452,126,126,,,3114598527,4531528684,4684815069,3031977480,2082452,ERX11454441,ERS16387683,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93661,0.936,0.17032,0.17368,0.7699,0.77157,0.74201,0.77289,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14915,ERR12071815,ERX11454440,ERS16387682,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 4,SAMEA114398990,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66673,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-4_S14_L004_R1_001.fastq.gz 109-4_S14_L004_R2_001.fastq.gz,fastq fastq,17693786880.0,70213440.0,ena RUN TAB 21 09 2023 13:32:15:289 66674,,,,,,,,,,,,ERX11454440,ERS16387682,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93895,0.93626,0.13454,0.13567,0.80679,0.80906,0.83187,0.85713,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14916,ERR12071814,ERX11454439,ERS16387681,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 3,SAMEA114398989,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66671,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-3_S6_L002_R1_001.fastq.gz 109-3_S6_L002_R2_001.fastq.gz,fastq fastq,15326268300.0,60818525.0,ena RUN TAB 21 09 2023 13:32:15:289 66672,0:126 1:126,A:2963028716;C:4693248657;G:4853138006;T:2814772075;N:2080846,126,126,,,2963028716,4693248657,4853138006,2814772075,2080846,ERX11454439,ERS16387681,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95492,0.95516,0.18592,0.18766,0.816,0.81722,0.82037,0.77416,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14917,ERR12071813,ERX11454438,ERS16387680,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 2,SAMEA114398988,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:289 66669,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-2_S9_L003_R1_001.fastq.gz 109-2_S9_L003_R2_001.fastq.gz,fastq fastq,13463300088.0,53425794.0,ena RUN TAB 21 09 2023 13:32:15:289 66670,0:126 1:126,A:2565791374;C:4152722401;G:4318203775;T:2424775489;N:1807049,126,126,,,2565791374,4152722401,4318203775,2424775489,1807049,ERX11454438,ERS16387680,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.96145,0.9621,0.19448,0.19497,0.83382,0.83451,0.84292,0.79683,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14918,ERR12071812,ERX11454437,ERS16387679,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E109 1,SAMEA114398987,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:109 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:109 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66667,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,109-1_S1_L001_R2_001.fastq.gz 109-1_S1_L001_R1_001.fastq.gz,fastq fastq,16254330624.0,64501312.0,ena RUN TAB 21 09 2023 13:32:15:289 66668,0:126 1:126,A:3146898181;C:4962037311;G:5139473645;T:3002518241;N:3403246,126,126,,,3146898181,4962037311,5139473645,3002518241,3403246,ERX11454437,ERS16387679,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95901,0.9593,0.19067,0.19204,0.8174,0.81864,0.7987,0.8132,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14919,ERR12071811,ERX11454436,ERS16387678,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 4,SAMEA114398986,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66665,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-4_S10_L003_R1_001.fastq.gz 108-4_S10_L003_R2_001.fastq.gz,fastq fastq,16401717108.0,65086179.0,ena RUN TAB 21 09 2023 13:32:15:288 66666,0:126 1:126,A:3306091376;C:4834896468;G:4995683070;T:3262829888;N:2216306,126,126,,,3306091376,4834896468,4995683070,3262829888,2216306,ERX11454436,ERS16387678,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92612,0.92543,0.16352,0.16724,0.76607,0.76812,0.76693,0.75698,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14920,ERR12071810,ERX11454435,ERS16387677,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 3,SAMEA114398985,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66663,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-3_S2_L001_R2_001.fastq.gz 108-3_S2_L001_R1_001.fastq.gz,fastq fastq,14063114268.0,55806009.0,ena RUN TAB 21 09 2023 13:32:15:288 66664,0:126 1:126,A:2855140106;C:4126986255;G:4254731693;T:2823386830;N:2869384,126,126,,,2855140106,4126986255,4254731693,2823386830,2869384,ERX11454435,ERS16387677,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89956,0.89846,0.17394,0.17665,0.77695,0.77751,0.78333,0.77908,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14921,ERR12071809,ERX11454434,ERS16387676,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 2,SAMEA114398984,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:288 66661,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-2_S13_L004_R1_001.fastq.gz 108-2_S13_L004_R2_001.fastq.gz,fastq fastq,17951370444.0,71235597.0,ena RUN TAB 21 09 2023 13:32:15:288 66662,0:126 1:126,A:3419406758;C:5496702371;G:5719743964;T:3312990750;N:2526601,126,126,,,3419406758,5496702371,5719743964,3312990750,2526601,ERX11454434,ERS16387676,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91308,0.91164,0.14161,0.14408,0.78725,0.78851,0.77166,0.81536,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14922,ERR12071808,ERX11454433,ERS16387675,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E108 1,SAMEA114398983,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:108 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:108 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66659,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,108-1_S5_L002_R1_001.fastq.gz 108-1_S5_L002_R2_001.fastq.gz,fastq fastq,17592295140.0,69810695.0,ena RUN TAB 21 09 2023 13:32:15:288 66660,0:126 1:126,A:3473054128;C:5265562453;G:5455395791;T:3395881406;N:2401362,126,126,,,3473054128,5265562453,5455395791,3395881406,2401362,ERX11454433,ERS16387675,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92632,0.92464,0.16285,0.16525,0.7781,0.77985,0.79539,0.79841,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14923,ERR12071807,ERX11454432,ERS16387674,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 4,SAMEA114398982,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66657,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-4_S20_L005_R1_001.fastq.gz 107-4_S20_L005_R2_001.fastq.gz,fastq fastq,12626647992.0,50105746.0,ena RUN TAB 21 09 2023 13:32:15:287 66658,0:126 1:126,A:2503116695;C:3776878144;G:3904732537;T:2438634661;N:3285955,126,126,,,2503116695,3776878144,3904732537,2438634661,3285955,ERX11454432,ERS16387674,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93409,0.93399,0.16467,0.16803,0.77567,0.77749,0.77962,0.76236,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14924,ERR12071806,ERX11454431,ERS16387673,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 3,SAMEA114398981,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66655,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-3_S28_L007_R1_001.fastq.gz 107-3_S28_L007_R2_001.fastq.gz,fastq fastq,19765610928.0,78434964.0,ena RUN TAB 21 09 2023 13:32:15:287 66656,0:126 1:126,A:3807913538;C:6041902918;G:6270499946;T:3639644996;N:5649530,126,126,,,3807913538,6041902918,6270499946,3639644996,5649530,ERX11454431,ERS16387673,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93479,0.93431,0.15154,0.15299,0.78462,0.78533,0.78429,0.79385,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14925,ERR12071805,ERX11454430,ERS16387672,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 2,SAMEA114398980,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:287 66653,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-2_S32_L008_R1_001.fastq.gz 107-2_S32_L008_R2_001.fastq.gz,fastq fastq,20245786344.0,80340422.0,ena RUN TAB 21 09 2023 13:32:15:287 66654,0:126 1:126,A:3580212812;C:6483019868;G:6732117106;T:3443314781;N:7121777,126,126,,,3580212812,6483019868,6732117106,3443314781,7121777,ERX11454430,ERS16387672,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93526,0.93431,0.14042,0.14264,0.82887,0.83041,0.85552,0.85524,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14926,ERR12071804,ERX11454429,ERS16387671,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E107 1,SAMEA114398979,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:107 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:107 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66651,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,107-1_S24_L006_R2_001.fastq.gz 107-1_S24_L006_R1_001.fastq.gz,fastq fastq,15192276120.0,60286810.0,ena RUN TAB 21 09 2023 13:32:15:286 66652,0:126 1:126,A:2840014487;C:4709824279;G:4867364305;T:2771588673;N:3484376,126,126,,,2840014487,4709824279,4867364305,2771588673,3484376,ERX11454429,ERS16387671,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92039,0.91914,0.17007,0.17237,0.80939,0.80986,0.84885,0.847,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14927,ERR12071803,ERX11454428,ERS16387670,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 4,SAMEA114398978,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66649,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-4_S23_L006_R2_001.fastq.gz 106-4_S23_L006_R1_001.fastq.gz,fastq fastq,16985562300.0,67403025.0,ena RUN TAB 21 09 2023 13:32:15:286 66650,0:126 1:126,A:3322275260;C:5133904780;G:5334495158;T:3190965461;N:3921641,126,126,,,3322275260,5133904780,5334495158,3190965461,3921641,ERX11454428,ERS16387670,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93707,0.93661,0.15576,0.15861,0.77826,0.78025,0.77722,0.77979,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14928,ERR12071802,ERX11454427,ERS16387669,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 3,SAMEA114398977,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:286 66647,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-3_S31_L008_R2_001.fastq.gz 106-3_S31_L008_R1_001.fastq.gz,fastq fastq,19459285524.0,77219387.0,ena RUN TAB 21 09 2023 13:32:15:286 66648,0:126 1:126,A:3823996295;C:5872737349;G:6122836793;T:3632881865;N:6833222,126,126,,,3823996295,5872737349,6122836793,3632881865,6833222,ERX11454427,ERS16387669,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90902,0.90816,0.1544,0.15713,0.78926,0.79176,0.76867,0.78913,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14929,ERR12071801,ERX11454426,ERS16387668,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 2,SAMEA114398976,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66645,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-2_S27_L007_R1_001.fastq.gz 106-2_S27_L007_R2_001.fastq.gz,fastq fastq,15694418124.0,62279437.0,ena RUN TAB 21 09 2023 13:32:15:286 66646,0:126 1:126,A:3002717019;C:4829058190;G:5037608362;T:2820533482;N:4501071,126,126,,,3002717019,4829058190,5037608362,2820533482,4501071,ERX11454426,ERS16387668,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93511,0.93528,0.14004,0.14226,0.7891,0.79068,0.77063,0.77891,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14930,ERR12071800,ERX11454425,ERS16387667,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E106 1,SAMEA114398975,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:106 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:106 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66643,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,106-1_S19_L005_R1_001.fastq.gz 106-1_S19_L005_R2_001.fastq.gz,fastq fastq,18647636868.0,73998559.0,ena RUN TAB 21 09 2023 13:32:15:285 66644,0:126 1:126,A:3692341453;C:5587227660;G:5803402834;T:3559657331;N:5007590,126,126,,,3692341453,5587227660,5803402834,3559657331,5007590,ERX11454425,ERS16387667,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92714,0.92688,0.16214,0.16642,0.77433,0.775,0.76217,0.75322,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14931,ERR12071799,ERX11454424,ERS16387666,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 4,SAMEA114398974,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66641,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-4_S26_L007_R2_001.fastq.gz 105-4_S26_L007_R1_001.fastq.gz,fastq fastq,15124522392.0,60017946.0,ena RUN TAB 21 09 2023 13:32:15:285 66642,0:126 1:126,A:2852895871;C:4689063320;G:4867825391;T:2710443233;N:4294577,126,126,,,2852895871,4689063320,4867825391,2710443233,4294577,ERX11454424,ERS16387666,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95529,0.95479,0.13148,0.13281,0.7793,0.78125,0.78337,0.78508,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14932,ERR12071798,ERX11454423,ERS16387665,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 3,SAMEA114398973,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:285 66639,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-3_S18_L005_R1_001.fastq.gz 105-3_S18_L005_R2_001.fastq.gz,fastq fastq,17644367664.0,70017332.0,ena RUN TAB 21 09 2023 13:32:15:285 66640,0:126 1:126,A:3347478814;C:5457952160;G:5642901286;T:3191260656;N:4774748,126,126,,,3347478814,5457952160,5642901286,3191260656,4774748,ERX11454423,ERS16387665,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94866,0.94893,0.14303,0.14517,0.78658,0.78697,0.80916,0.81131,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14933,ERR12071797,ERX11454422,ERS16387664,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 2,SAMEA114398972,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66637,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-2_S30_L008_R1_001.fastq.gz 105-2_S30_L008_R2_001.fastq.gz,fastq fastq,15981084252.0,63417001.0,ena RUN TAB 21 09 2023 13:32:15:285 66638,0:126 1:126,A:3059876138;C:4898463877;G:5076925794;T:2940239108;N:5579335,126,126,,,3059876138,4898463877,5076925794,2940239108,5579335,ERX11454422,ERS16387664,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.95109,0.95022,0.14624,0.14776,0.78066,0.78283,0.76738,0.79238,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14934,ERR12071796,ERX11454421,ERS16387663,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E105 1,SAMEA114398971,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:105 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:105 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66635,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,105-1_S22_L006_R1_001.fastq.gz 105-1_S22_L006_R2_001.fastq.gz,fastq fastq,16378597368.0,64994434.0,ena RUN TAB 21 09 2023 13:32:15:284 66636,0:126 1:126,A:3157768088;C:4992606167;G:5190605482;T:3033853059;N:3764572,126,126,,,3157768088,4992606167,5190605482,3033853059,3764572,ERX11454421,ERS16387663,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9472,0.94619,0.15768,0.15997,0.78646,0.78845,0.75864,0.79159,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14935,ERR12071795,ERX11454420,ERS16387662,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 4,SAMEA114398970,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66633,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-4_S29_L008_R2_001.fastq.gz 104-4_S29_L008_R1_001.fastq.gz,fastq fastq,17713234476.0,70290613.0,ena RUN TAB 21 09 2023 13:32:15:284 66634,0:126 1:126,A:3387663411;C:5419734351;G:5646953646;T:3252634316;N:6248752,126,126,,,3387663411,5419734351,5646953646,3252634316,6248752,ERX11454420,ERS16387662,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91957,0.91797,0.14938,0.15201,0.79149,0.79358,0.7508,0.80456,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14936,ERR12071794,ERX11454419,ERS16387661,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 3,SAMEA114398969,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:284 66631,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-3_S21_L006_R1_001.fastq.gz 104-3_S21_L006_R2_001.fastq.gz,fastq fastq,20412904428.0,81003589.0,ena RUN TAB 21 09 2023 13:32:15:284 66632,0:126 1:126,A:3999139733;C:6180998229;G:6441889554;T:3786215014;N:4661898,126,126,,,3999139733,6180998229,6441889554,3786215014,4661898,ERX11454419,ERS16387661,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93627,0.93494,0.14845,0.14979,0.77796,0.77891,0.76434,0.77202,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14937,ERR12071793,ERX11454418,ERS16387660,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 2,SAMEA114398968,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66629,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-2_S25_L007_R1_001.fastq.gz 104-2_S25_L007_R2_001.fastq.gz,fastq fastq,15067973340.0,59793545.0,ena RUN TAB 21 09 2023 13:32:15:284 66630,0:126 1:126,A:2963040605;C:4540311477;G:4736958045;T:2823360579;N:4302634,126,126,,,2963040605,4540311477,4736958045,2823360579,4302634,ERX11454418,ERS16387660,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92968,0.92854,0.16053,0.16248,0.78224,0.78228,0.7365,0.76845,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14938,ERR12071792,ERX11454417,ERS16387659,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E104 1,SAMEA114398967,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:104 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:104 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66627,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,104-1_S17_L005_R1_001.fastq.gz 104-1_S17_L005_R2_001.fastq.gz,fastq fastq,19257494508.0,76418629.0,ena RUN TAB 21 09 2023 13:32:15:283 66628,0:126 1:126,A:3731156408;C:5859411051;G:6100994986;T:3560909583;N:5022480,126,126,,,3731156408,5859411051,6100994986,3560909583,5022480,ERX11454417,ERS16387659,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92633,0.92586,0.14768,0.14948,0.78277,0.7833,0.7452,0.76737,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14939,ERR12071791,ERX11454416,ERS16387658,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 4,SAMEA114398966,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66625,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-4_S32_L006_R1_001.fastq.gz 103-4_S32_L006_R2_001.fastq.gz,fastq fastq,13085486820.0,51926535.0,ena RUN TAB 21 09 2023 13:32:15:283 66626,0:126 1:126,A:2567103783;C:3969617611;G:4069486176;T:2476759371;N:2519879,126,126,,,2567103783,3969617611,4069486176,2476759371,2519879,ERX11454416,ERS16387658,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.93075,0.93242,0.14151,0.14331,0.774,0.76822,0.75401,0.76454,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14940,ERR12071790,ERX11454415,ERS16387657,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 3,SAMEA114398965,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:283 66623,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-3_S65_L007_R2_001.fastq.gz 103-3_S65_L007_R1_001.fastq.gz,fastq fastq,11192352696.0,44414098.0,ena RUN TAB 21 09 2023 13:32:15:283 66624,0:126 1:126,A:2123359510;C:3455439467;G:3570545494;T:2039990322;N:3017903,126,126,,,2123359510,3455439467,3570545494,2039990322,3017903,ERX11454415,ERS16387657,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89542,0.89591,0.1351,0.13661,0.78948,0.79003,0.77414,0.80393,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14941,ERR12071789,ERX11454414,ERS16387656,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 2,SAMEA114398964,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66621,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-2_S36_L007_R1_001.fastq.gz 103-2_S36_L007_R2_001.fastq.gz,fastq fastq,10568377512.0,41938006.0,ena RUN TAB 21 09 2023 13:32:15:283 66622,0:126 1:126,A:2109516452;C:3179785410;G:3272667938;T:2004453838;N:1953874,126,126,,,2109516452,3179785410,3272667938,2004453838,1953874,ERX11454414,ERS16387656,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92729,0.92817,0.15094,0.1528,0.7685,0.76516,0.74143,0.74277,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14942,ERR12071788,ERX11454413,ERS16387655,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E103 1,SAMEA114398963,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:103 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:103 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66619,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,103-1_S69_L008_R1_001.fastq.gz 103-1_S69_L008_R2_001.fastq.gz,fastq fastq,9417798180.0,37372215.0,ena RUN TAB 21 09 2023 13:32:15:282 66620,0:126 1:126,A:1807793384;C:2891759578;G:2983948236;T:1730405095;N:3891887,126,126,,,1807793384,2891759578,2983948236,1730405095,3891887,ERX11454413,ERS16387655,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.8784,0.87802,0.13342,0.13445,0.789,0.78946,0.79547,0.79885,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14943,ERR12071787,ERX11454412,ERS16387654,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 4,SAMEA114398962,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66617,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-4_S35_L007_R2_001.fastq.gz 102-4_S35_L007_R1_001.fastq.gz,fastq fastq,15605254728.0,61925614.0,ena RUN TAB 21 09 2023 13:32:15:282 66618,0:126 1:126,A:3158918177;C:4616749707;G:4726409583;T:3100328707;N:2848554,126,126,,,3158918177,4616749707,4726409583,3100328707,2848554,ERX11454412,ERS16387654,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91674,0.91732,0.1668,0.16991,0.77569,0.77082,0.76006,0.76056,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14944,ERR12071786,ERX11454411,ERS16387653,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 3,SAMEA114398961,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:282 66615,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-3_S68_L008_R1_001.fastq.gz 102-3_S68_L008_R2_001.fastq.gz,fastq fastq,20642076252.0,81913001.0,ena RUN TAB 21 09 2023 13:32:15:282 66616,0:126 1:126,A:3670199430;C:6557337612;G:6776033266;T:3629933781;N:8572163,126,126,,,3670199430,6557337612,6776033266,3629933781,8572163,ERX11454411,ERS16387653,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.89984,0.89794,0.14393,0.14603,0.82079,0.82233,0.83939,0.8347,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14945,ERR12071785,ERX11454410,ERS16387652,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 2,SAMEA114398960,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66613,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 22,102-2_S31_L006_R1_001.fastq.gz 102-2_S31_L006_R2_001.fastq.gz,fastq fastq,23877621936.0,94752468.0,ena RUN TAB 21 09 2023 13:32:15:282 66614,0:126 1:126,A:3953279642;C:7876070920;G:8128759951;T:3914909602;N:4601821,126,126,,,3953279642,7876070920,8128759951,3914909602,4601821,ERX11454410,ERS16387652,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92602,0.85538,0.13016,0.12286,0.84508,0.84936,0.81613,0.88529,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14946,ERR12071784,ERX11454409,ERS16387651,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,Low stress,E102 1,SAMEA114398959,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:102 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:102 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66611,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,102-1_S64_L007_R1_001.fastq.gz 102-1_S64_L007_R2_001.fastq.gz,fastq fastq,20588587236.0,81700743.0,ena RUN TAB 21 09 2023 13:32:15:281 66612,0:126 1:126,A:3005365881;C:7167599347;G:7450281822;T:2959758690;N:5581496,126,126,,,3005365881,7167599347,7450281822,2959758690,5581496,ERX11454409,ERS16387651,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90225,0.89979,0.08909,0.09003,0.87071,0.87148,0.90162,0.83246,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14947,ERR12071783,ERX11454408,ERS16387650,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 4,SAMEA114398958,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66609,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-4_S30_L006_R1_001.fastq.gz 101-4_S30_L006_R2_001.fastq.gz,fastq fastq,12524862168.0,49701834.0,ena RUN TAB 21 09 2023 13:32:15:281 66610,0:126 1:126,A:2576891018;C:3688938717;G:3819872503;T:2436717421;N:2442509,126,126,,,2576891018,3688938717,3819872503,2436717421,2442509,ERX11454408,ERS16387650,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92878,0.92997,0.15322,0.15505,0.76315,0.76063,0.69783,0.67569,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14948,ERR12071782,ERX11454407,ERS16387649,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 3,SAMEA114398957,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:281 66607,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-3_S34_L007_R1_001.fastq.gz 101-3_S34_L007_R2_001.fastq.gz,fastq fastq,11973395700.0,47513475.0,ena RUN TAB 21 09 2023 13:32:15:281 66608,0:126 1:126,A:2371769443;C:3605917515;G:3700919970;T:2292577579;N:2211193,126,126,,,2371769443,3605917515,3700919970,2292577579,2211193,ERX11454407,ERS16387649,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.92217,0.92315,0.14568,0.1469,0.7724,0.76917,0.7521,0.75171,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14949,ERR12071781,ERX11454406,ERS16387648,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 2,SAMEA114398956,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66605,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-2_S63_L007_R2_001.fastq.gz 101-2_S63_L007_R1_001.fastq.gz,fastq fastq,10806069456.0,42881228.0,ena RUN TAB 21 09 2023 13:32:15:280 66606,0:126 1:126,A:2073944191;C:3321784426;G:3405352767;T:2002070301;N:2917771,126,126,,,2073944191,3321784426,3405352767,2002070301,2917771,ERX11454406,ERS16387648,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9236,0.92427,0.14372,0.14539,0.78084,0.78141,0.74294,0.78886,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14950,ERR12071780,ERX11454405,ERS16387647,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E101 1,SAMEA114398955,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:101 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:101 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66603,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,101-1_S67_L008_R1_001.fastq.gz 101-1_S67_L008_R2_001.fastq.gz,fastq fastq,17155416348.0,68077049.0,ena RUN TAB 21 09 2023 13:32:15:280 66604,0:126 1:126,A:3066178934;C:5490109793;G:5728819123;T:2863162486;N:7146012,126,126,,,3066178934,5490109793,5728819123,2863162486,7146012,ERX11454405,ERS16387647,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.94204,0.94154,0.12549,0.1266,0.80653,0.80726,0.83737,0.8394,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14951,ERR12071779,ERX11454404,ERS16387646,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 4,SAMEA114398954,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 4|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 4|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66601,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-4_S33_L007_R1_001.fastq.gz 100-4_S33_L007_R2_001.fastq.gz,fastq fastq,20883396240.0,82870620.0,ena RUN TAB 21 09 2023 13:32:15:280 66602,0:126 1:126,A:4151890640;C:6231825391;G:6398810174;T:4097014520;N:3855515,126,126,,,4151890640,6231825391,6398810174,4097014520,3855515,ERX11454404,ERS16387646,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.91603,0.9155,0.17576,0.1788,0.7781,0.7753,0.76044,0.78007,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14952,ERR12071778,ERX11454403,ERS16387645,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 3,SAMEA114398953,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 3|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 3|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:280 66599,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-3_S29_L006_R1_001.fastq.gz 100-3_S29_L006_R2_001.fastq.gz,fastq fastq,17203126752.0,68266376.0,ena RUN TAB 21 09 2023 13:32:15:280 66600,0:126 1:126,A:3380289058;C:5172410817;G:5314572798;T:3332505592;N:3348487,126,126,,,3380289058,5172410817,5314572798,3332505592,3348487,ERX11454403,ERS16387645,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.90457,0.90462,0.18582,0.18981,0.79947,0.79435,0.81648,0.81903,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14953,ERR12071777,ERX11454402,ERS16387644,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 2,SAMEA114398952,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 2|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 2|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:279 66597,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-2_S66_L008_R2_001.fastq.gz 100-2_S66_L008_R1_001.fastq.gz,fastq fastq,12181472100.0,48339175.0,ena RUN TAB 21 09 2023 13:32:15:279 66598,0:126 1:126,A:2169455038;C:3847243791;G:3951410714;T:2208297402;N:5065155,126,126,,,2169455038,3847243791,3951410714,2208297402,5065155,ERX11454402,ERS16387644,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.87873,0.8759,0.15894,0.16003,0.82446,0.82475,0.8538,0.82928,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise 14954,ERR12071776,ERX11454401,ERS16387643,ERP151293,PRJEB66218,Social stress in Zebrafish sperm,b81525de-0f4f-4e41-8e34-f411e5590e7b,Other,Environmental changes may affect paternal condition and following generations but the underlying transmission mechanisms of such information is unknown. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and hatch rates in their offspring. Here we investigated the potential role of small RNAs in sperm in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes at this key developmental stage. We sequenced mi and piRNAs and the full transcriptome in the resulting offspring and ran a differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from a high to a low competition environment. We identified 612 differentially expressed genes in the embryos. The changes in gene expression in the embryos support the idea of faster development and hatching and can be linked to some of the differentially expressed small RNAs in sperm.,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,,High stress,E100 1,SAMEA114398951,University of East Anglia,INSDC center name:University of East Anglia|Submitter Id:100 1|collection date:2014|common name:zebrafish|geographic location country and/or sea:Sweden|sample name:100 1|scientific name:Danio rerio,,,,,,,,,Illumina HiSeq X paired end sequencing,ena EXPERIMENT TAB 21 09 2023 13:32:15:279 66595,125bp,1,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq X,,ERP151293,Illumina HiSeq X paired end sequencing,ENA FIRST PUBLIC:2023 09 21|ENA LAST UPDATE:2023 09 21,100-1_S62_L007_R1_001.fastq.gz 100-1_S62_L007_R2_001.fastq.gz,fastq fastq,12598877592.0,49995546.0,ena RUN TAB 21 09 2023 13:32:15:279 66596,0:126 1:126,A:2443108338;C:3813175879;G:3922667630;T:2416483718;N:3442027,126,126,,,2443108338,3813175879,3922667630,2416483718,3442027,ERX11454401,ERS16387643,ERA27252154,university of east anglia|European Nucleotide Archive,university of east anglia,2,0.9126,0.91287,0.18702,0.1895,0.79431,0.79373,0.78056,0.81778,126,126,B,B,biological fallback assumption,illumina,hiseq_era,unknown,small_rna,unknown,bulk,unknown,unknown,,United Kingdom,2023-09-21,Undetermined,Embryo,Undetermined,Embryo Imprecise