rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 11025,ERR9787516,ERX9334792,ERS12088364,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 9,SAMEA14460887,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460887|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:24Z|INSDC last update:2022 09 12T12:19:24Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 9|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 9|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 9 p,Sample 9 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,TSCHOM1_1.fastq.gz TSCHOM1_2.fastq.gz,fastq fastq,6360351174.0,31486887.0,E MTAB 11776:TSCHOM1 ,0:101 1:101,A:1750731340;C:1410426576;G:1460021447;T:1739131898;N:39913,101,101,,,1750731340,1410426576,1460021447,1739131898,39913,ERX9334792,ERS12088364,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93517,0.92417,0.15551,0.15199,0.67736,0.6786,0.48763,0.48988,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11026,ERR9787515,ERX9334791,ERS12088363,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 8,SAMEA14460886,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460886|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:24Z|INSDC last update:2022 09 12T12:19:24Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 8|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 8|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 8 p,Sample 8 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,DEPHOM4_1.fastq.gz DEPHOM4_2.fastq.gz,fastq fastq,6195443828.0,30670514.0,E MTAB 11776:DEPHOM4 ,0:101 1:101,A:1524344281;C:1573560980;G:1585093004;T:1512406843;N:38720,101,101,,,1524344281,1573560980,1585093004,1512406843,38720,ERX9334791,ERS12088363,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.95088,0.9542,0.17631,0.17203,0.71121,0.70981,0.56015,0.57999,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11027,ERR9787514,ERX9334790,ERS12088362,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 7,SAMEA14460885,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460885|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 7|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 7|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 7 p,Sample 7 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,DEPHOM7_1.fastq.gz DEPHOM7_2.fastq.gz,fastq fastq,6201660378.0,30701289.0,E MTAB 11776:DEPHOM7 ,0:101 1:101,A:1699906420;C:1382805098;G:1427335171;T:1691574092;N:39597,101,101,,,1699906420,1382805098,1427335171,1691574092,39597,ERX9334790,ERS12088362,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.92781,0.92603,0.15556,0.15416,0.67965,0.67963,0.48209,0.48229,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11028,ERR9787513,ERX9334789,ERS12088361,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 6,SAMEA14460884,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460884|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 6|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 6 p,Sample 6 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,DEPHOM6_1.fastq.gz DEPHOM6_2.fastq.gz,fastq fastq,6255857382.0,30969591.0,E MTAB 11776:DEPHOM6 ,0:101 1:101,A:1722554517;C:1398134491;G:1424183345;T:1710945949;N:39080,101,101,,,1722554517,1398134491,1424183345,1710945949,39080,ERX9334789,ERS12088361,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93476,0.93206,0.16121,0.1592,0.67882,0.67898,0.48043,0.47851,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11029,ERR9787512,ERX9334788,ERS12088360,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 5,SAMEA14460883,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460883|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 5|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 5 p,Sample 5 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,DEPHOM1_1.fastq.gz DEPHOM1_2.fastq.gz,fastq fastq,6340417006.0,31388203.0,E MTAB 11776:DEPHOM1 ,0:101 1:101,A:1738622221;C:1420060351;G:1455477148;T:1726216833;N:40453,101,101,,,1738622221,1420060351,1455477148,1726216833,40453,ERX9334788,ERS12088360,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93634,0.93167,0.15036,0.14813,0.67809,0.67813,0.47978,0.47851,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11030,ERR9787511,ERX9334787,ERS12088359,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 4,SAMEA14460882,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460882|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 4|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 4 p,Sample 4 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / x tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,2XHOM7_1.fastq.gz 2XHOM7_2.fastq.gz,fastq fastq,6291880446.0,31147923.0,E MTAB 11776:2XHOM7 ,0:101 1:101,A:1712784902;C:1424143534;G:1452943449;T:1701969086;N:39475,101,101,,,1712784902,1424143534,1452943449,1701969086,39475,ERX9334787,ERS12088359,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93869,0.93511,0.15405,0.15042,0.67377,0.67351,0.50599,0.50683,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11031,ERR9787510,ERX9334786,ERS12088358,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 3,SAMEA14460881,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460881|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 3|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 3 p,Sample 3 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / x tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,2XHOM6_1.fastq.gz 2XHOM6_2.fastq.gz,fastq fastq,6214327596.0,30763998.0,E MTAB 11776:2XHOM6 ,0:101 1:101,A:1705019759;C:1384064144;G:1432455427;T:1692749964;N:38302,101,101,,,1705019759,1384064144,1432455427,1692749964,38302,ERX9334786,ERS12088358,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93591,0.92636,0.14492,0.14145,0.67489,0.67491,0.47753,0.49286,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11032,ERR9787509,ERX9334785,ERS12088357,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 2,SAMEA14460880,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460880|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 2|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 2 p,Sample 2 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / x tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,2XHOM4_1.fastq.gz 2XHOM4_2.fastq.gz,fastq fastq,6301691990.0,31196495.0,E MTAB 11776:2XHOM4 ,0:101 1:101,A:1698118705;C:1442276237;G:1473988715;T:1687268823;N:39510,101,101,,,1698118705,1442276237,1473988715,1687268823,39510,ERX9334785,ERS12088357,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.94255,0.93839,0.13232,0.13007,0.67326,0.67294,0.49084,0.49291,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11033,ERR9787508,ERX9334784,ERS12088356,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 16,SAMEA14460879,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460879|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 16|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 16|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 16 p,Sample 16 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,WT7_1.fastq.gz WT7_2.fastq.gz,fastq fastq,6206666746.0,30726073.0,E MTAB 11776:WT7 ,0:101 1:101,A:1705689174;C:1387290312;G:1420249657;T:1693397929;N:39674,101,101,,,1705689174,1387290312,1420249657,1693397929,39674,ERX9334784,ERS12088356,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93493,0.92964,0.15803,0.1554,0.67405,0.67501,0.47988,0.46768,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11034,ERR9787507,ERX9334783,ERS12088355,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 15,SAMEA14460878,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460878|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 15|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 15|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 15 p,Sample 15 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,WT6_1.fastq.gz WT6_2.fastq.gz,fastq fastq,6360740630.0,31488815.0,E MTAB 11776:WT6 ,0:101 1:101,A:1735195643;C:1436805269;G:1469558083;T:1719141699;N:39936,101,101,,,1735195643,1436805269,1469558083,1719141699,39936,ERX9334783,ERS12088355,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93629,0.93343,0.15169,0.1501,0.67572,0.67517,0.47499,0.47879,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11035,ERR9787506,ERX9334782,ERS12088354,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 14,SAMEA14460877,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460877|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 14|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 14|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 14 p,Sample 14 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,WT4_1.fastq.gz WT4_2.fastq.gz,fastq fastq,6227472342.0,30829071.0,E MTAB 11776:WT4 ,0:101 1:101,A:1668352581;C:1434152947;G:1468645621;T:1656281986;N:39207,101,101,,,1668352581,1434152947,1468645621,1656281986,39207,ERX9334782,ERS12088354,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.94261,0.93675,0.15334,0.15049,0.67927,0.67872,0.48762,0.49138,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11036,ERR9787505,ERX9334781,ERS12088353,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 13,SAMEA14460876,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460876|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 13|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 13|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 13 p,Sample 13 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,WT1_1.fastq.gz WT1_2.fastq.gz,fastq fastq,6332757570.0,31350285.0,E MTAB 11776:WT1 ,0:101 1:101,A:1726838066;C:1431452165;G:1460746594;T:1713681043;N:39702,101,101,,,1726838066,1431452165,1460746594,1713681043,39702,ERX9334781,ERS12088353,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.92422,0.93556,0.14884,0.14926,0.67694,0.67564,0.48223,0.48884,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11037,ERR9787504,ERX9334780,ERS12088352,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 12,SAMEA14460875,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460875|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 12|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 12|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 12 p,Sample 12 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,TSCHOM7_1.fastq.gz TSCHOM7_2.fastq.gz,fastq fastq,6216990360.0,30777180.0,E MTAB 11776:TSCHOM7 ,0:101 1:101,A:1710256020;C:1390027205;G:1419250792;T:1697417099;N:39244,101,101,,,1710256020,1390027205,1419250792,1697417099,39244,ERX9334780,ERS12088352,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93162,0.92801,0.15191,0.14943,0.67789,0.67951,0.47915,0.47762,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11038,ERR9787503,ERX9334779,ERS12088351,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 11,SAMEA14460874,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460874|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 11|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 11|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 11 p,Sample 11 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,TSCHOM6_1.fastq.gz TSCHOM6_2.fastq.gz,fastq fastq,6199081848.0,30688524.0,E MTAB 11776:TSCHOM6 ,0:101 1:101,A:1705859045;C:1387530558;G:1415224718;T:1690428701;N:38826,101,101,,,1705859045,1387530558,1415224718,1690428701,38826,ERX9334779,ERS12088351,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93583,0.93349,0.15923,0.15664,0.67976,0.67986,0.48632,0.48376,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11039,ERR9787502,ERX9334778,ERS12088350,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 10,SAMEA14460873,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460873|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 10|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 10|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 10 p,Sample 10 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,TSCHOM4_1.fastq.gz TSCHOM4_2.fastq.gz,fastq fastq,6287836204.0,31127902.0,E MTAB 11776:TSCHOM4 ,0:101 1:101,A:1673762523;C:1453880149;G:1493343671;T:1666809849;N:40012,101,101,,,1673762523,1453880149,1493343671,1666809849,40012,ERX9334778,ERS12088350,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.94269,0.9375,0.14166,0.13979,0.67476,0.67722,0.4927,0.49365,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11040,ERR9787501,ERX9334777,ERS12088349,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 1,SAMEA14460872,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460872|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 1|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 1 p,Sample 1 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / x tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,2XHOM1_1.fastq.gz 2XHOM1_2.fastq.gz,fastq fastq,6232605768.0,30854484.0,E MTAB 11776:2XHOM1 ,0:101 1:101,A:1725401812;C:1384507160;G:1410306583;T:1712351429;N:38784,101,101,,,1725401812,1384507160,1410306583,1712351429,38784,ERX9334777,ERS12088349,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93594,0.93262,0.15282,0.14977,0.67503,0.67373,0.49522,0.49759,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 34192,SRR31443228,SRX26812023,SRS23296672,SRP546978,PRJNA1189474,Bulk RNA Seq of srrm4 homozygous mutant and wild type sibling larvae at 3 dpf,PRJNA1189474,Other,The goal of this project is to assess the effects of mutations in zebrafish srrm4 on alternative splicing of microexons. Homozygous mutant and homozygous wild type sibling larvae were identified from heterozygous parent crosses Total RNA was isolated for sequencing at 3 dpf,,,,,srrm4[y712] mutant replicate 3,,strain:Tpfel long fin|age:3 dpf|collection date:2022 02|geo loc name:USA: Bethesda|sex:not applicable|tissue:head and torso|genotype:homozygous mutant|Replicate:mutant 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,srrm4[y712] mutant replicate 3,srrm4[y712] mutant replicate 3,TruSeq library,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP546978,,assembly:GRCz11,25938_mut3.pass2Aligned.sortedByCoord.out.bam,bam,23466336192.0,117587529.0,25938 mut3.pass2Aligned.sortedByCoord.out.bam,0:100.13 1:100.08,A:6298034402;C:5433884683;G:5344985734;T:6388443446;N:987927,100,100,,,6298034402,5433884683,5344985734,6388443446,987927,SRX26812023,SRS23296672,SRA2019780,Eunice Kennedy Shriver National Institute of Child Health and Human Development|Division of Developmental Biology,Eunice Kennedy Shriver National Institute of Child Health and Human Development,2,0.95864,0.95894,0.10681,0.1052,0.65385,0.65403,0.46511,0.46252,99,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,bulk,bulk,,United States,2024-11-22,Larval,Larval,Head,Nervous System 34193,SRR31443229,SRX26812022,SRS23296671,SRP546978,PRJNA1189474,Bulk RNA Seq of srrm4 homozygous mutant and wild type sibling larvae at 3 dpf,PRJNA1189474,Other,The goal of this project is to assess the effects of mutations in zebrafish srrm4 on alternative splicing of microexons. Homozygous mutant and homozygous wild type sibling larvae were identified from heterozygous parent crosses Total RNA was isolated for sequencing at 3 dpf,,,,,srrm4[y712] wild type replicate 3,,strain:Tpfel long fin|age:3 dpf|collection date:2022 02|geo loc name:USA: Bethesda|sex:not applicable|tissue:head and torso|genotype:homozygous wild type|Replicate:wild type 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,srrm4[y712] wild type replicate 3,srrm4[y712] wild type replicate 3,TruSeq library,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP546978,,assembly:GRCz11,25938_wt3.pass2Aligned.sortedByCoord.out.bam,bam,23979171134.0,120156039.0,25938 wt3.pass2Aligned.sortedByCoord.out.bam,0:100.12 1:100.07,A:6398807397;C:5589997524;G:5503127729;T:6486232627;N:1005857,100,100,,,6398807397,5589997524,5503127729,6486232627,1005857,SRX26812022,SRS23296671,SRA2019780,Eunice Kennedy Shriver National Institute of Child Health and Human Development|Division of Developmental Biology,Eunice Kennedy Shriver National Institute of Child Health and Human Development,2,0.95941,0.96004,0.10323,0.10191,0.65451,0.6532,0.46396,0.46398,101,100,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,bulk,bulk,,United States,2024-11-22,Larval,Larval,Head,Nervous System 34194,SRR31443230,SRX26812021,SRS23296670,SRP546978,PRJNA1189474,Bulk RNA Seq of srrm4 homozygous mutant and wild type sibling larvae at 3 dpf,PRJNA1189474,Other,The goal of this project is to assess the effects of mutations in zebrafish srrm4 on alternative splicing of microexons. Homozygous mutant and homozygous wild type sibling larvae were identified from heterozygous parent crosses Total RNA was isolated for sequencing at 3 dpf,,,,,srrm4[y712] mutant replicate 2,,strain:Tpfel long fin|age:3 dpf|collection date:2022 02|geo loc name:USA: Bethesda|sex:not applicable|tissue:head and torso|genotype:homozygous mutant|Replicate:mutant 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,srrm4[y712] mutant replicate 2,srrm4[y712] mutant replicate 2,TruSeq library,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP546978,,assembly:GRCz11,25938_mut2.pass2Aligned.sortedByCoord.out.bam,bam,20256127500.0,101792067.0,25938 mut2.pass2Aligned.sortedByCoord.out.bam,0:100.05 1:100.00,A:5435798119;C:4683997479;G:4650293108;T:5485187428;N:851366,100,100,,,5435798119,4683997479,4650293108,5485187428,851366,SRX26812021,SRS23296670,SRA2019780,Eunice Kennedy Shriver National Institute of Child Health and Human Development|Division of Developmental Biology,Eunice Kennedy Shriver National Institute of Child Health and Human Development,2,0.96008,0.96049,0.10232,0.10156,0.66072,0.65932,0.4668,0.46817,95,95,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,bulk,bulk,,United States,2024-11-22,Larval,Larval,Head,Nervous System 34195,SRR31443231,SRX26812020,SRS23296669,SRP546978,PRJNA1189474,Bulk RNA Seq of srrm4 homozygous mutant and wild type sibling larvae at 3 dpf,PRJNA1189474,Other,The goal of this project is to assess the effects of mutations in zebrafish srrm4 on alternative splicing of microexons. Homozygous mutant and homozygous wild type sibling larvae were identified from heterozygous parent crosses Total RNA was isolated for sequencing at 3 dpf,,,,,srrm4[y712] wild type replicate 2,,strain:Tpfel long fin|age:3 dpf|collection date:2022 02|geo loc name:USA: Bethesda|sex:not applicable|tissue:head and torso|genotype:homozygous wild type|Replicate:wild type 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,srrm4[y712] wild type replicate 2,srrm4[y712] wild type replicate 2,TruSeq library,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP546978,,assembly:GRCz11,25938_wt2.pass2Aligned.sortedByCoord.out.bam,bam,22698373371.0,113599687.0,25938 wt2.pass2Aligned.sortedByCoord.out.bam,0:100.18 1:100.12,A:6063922145;C:5275724786;G:5212055837;T:6145709475;N:961128,100,100,,,6063922145,5275724786,5212055837,6145709475,961128,SRX26812020,SRS23296669,SRA2019780,Eunice Kennedy Shriver National Institute of Child Health and Human Development|Division of Developmental Biology,Eunice Kennedy Shriver National Institute of Child Health and Human Development,2,0.96129,0.96063,0.1003,0.09861,0.65744,0.65748,0.46167,0.46817,98,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,bulk,bulk,,United States,2024-11-22,Larval,Larval,Head,Nervous System 34196,SRR31443232,SRX26812019,SRS23296667,SRP546978,PRJNA1189474,Bulk RNA Seq of srrm4 homozygous mutant and wild type sibling larvae at 3 dpf,PRJNA1189474,Other,The goal of this project is to assess the effects of mutations in zebrafish srrm4 on alternative splicing of microexons. Homozygous mutant and homozygous wild type sibling larvae were identified from heterozygous parent crosses Total RNA was isolated for sequencing at 3 dpf,,,,,srrm4[y712] mutant replicate 1,,strain:Tpfel long fin|age:3 dpf|collection date:2021 06|geo loc name:USA: Bethesda|sex:not applicable|tissue:head and torso|genotype:homozygous mutant|Replicate:mutant 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,srrm4[y712] mutant replicate 1,srrm4[y712] mutant replicate 1,TruSeq library,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP546978,,assembly:GRCz11,25938_mut.pass2Aligned.sortedByCoord.out.bam,bam,11282501509.0,56702666.0,25938 mut.pass2Aligned.sortedByCoord.out.bam,0:99.65 1:99.55,A:2764745971;C:2868447715;G:2856237429;T:2792914545;N:155849,99,99,,,2764745971,2868447715,2856237429,2792914545,155849,SRX26812019,SRS23296667,SRA2019780,Eunice Kennedy Shriver National Institute of Child Health and Human Development|Division of Developmental Biology,Eunice Kennedy Shriver National Institute of Child Health and Human Development,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,bulk,bulk,,United States,2024-11-22,Larval,Larval,Head,Nervous System 34197,SRR31443233,SRX26812018,SRS23296668,SRP546978,PRJNA1189474,Bulk RNA Seq of srrm4 homozygous mutant and wild type sibling larvae at 3 dpf,PRJNA1189474,Other,The goal of this project is to assess the effects of mutations in zebrafish srrm4 on alternative splicing of microexons. Homozygous mutant and homozygous wild type sibling larvae were identified from heterozygous parent crosses Total RNA was isolated for sequencing at 3 dpf,,,,,srrm4[y712] wild type replicate 1,,strain:Tpfel long fin|age:3 dpf|collection date:2021 06|geo loc name:USA: Bethesda|sex:not applicable|tissue:head and torso|genotype:homozygous wild type|Replicate:wild type 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of zebrafish larvae,srrm4[y712] wild type replicate 1,srrm4[y712] wild type replicate 1,TruSeq library,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,SRP546978,,assembly:GRCz11,25938_wt.pass2Aligned.sortedByCoord.out.bam,bam,16346812838.0,82104499.0,25938 wt.pass2Aligned.sortedByCoord.out.bam,0:99.65 1:99.56,A:3996717287;C:4167381503;G:4130586142;T:4051899914;N:227992,99,99,,,3996717287,4167381503,4130586142,4051899914,227992,SRX26812018,SRS23296668,SRA2019780,Eunice Kennedy Shriver National Institute of Child Health and Human Development|Division of Developmental Biology,Eunice Kennedy Shriver National Institute of Child Health and Human Development,2,0.97776,0.97907,0.04095,0.04006,0.6702,0.67034,0.46611,0.46144,99,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,bulk,bulk,,United States,2024-11-22,Larval,Larval,Head,Nervous System 39641,SRR2029856,SRX1030037,SRS938363,SRP058038,PRJNA283169,Danio rerio Raw sequence reads,PRJNA283169,Other,RNA sequencing of whole head and Hcrt cells of 6dpf larvae zebrafish.,,,RNA was purified from WT 6dpf zebrafish larvae,WT whole head larva zebrafish,WT,,breed:AB|dev stage:6 dpf|sex:pooled male and female|tissue:whole head|BioSampleModel:Model organism or animal,,,,,,,,,Transcriptional profiling of hypocretin neurons identifies the sleep regulator Kcnh4a,WT 5,WT 5,Biological replicate 5,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,SRP058038,,,Sample_ii-na_2.fastq Sample_ii-na_1.fastq,fastq fastq,4970948000.0,24854740.0,WT 5,0:100 1:100,A:1326071248;C:1167369974;G:1133391102;T:1318946364;N:25169312,100,100,,,1326071248,1167369974,1133391102,1318946364,25169312,SRX1030037,SRS938363,SRA266465,Bar Ilan University|Dr. Lior Appelbaum,Bar Ilan University,2,0.93711,0.93844,0.07857,0.07934,0.64613,0.64711,0.47852,0.47693,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Israel,2016-05-06,Larval,Larval,Head,Nervous System 39642,SRR2025058,SRX1030036,SRS938363,SRP058038,PRJNA283169,Danio rerio Raw sequence reads,PRJNA283169,Other,RNA sequencing of whole head and Hcrt cells of 6dpf larvae zebrafish.,,,RNA was purified from WT 6dpf zebrafish larvae,WT whole head larva zebrafish,WT,,breed:AB|dev stage:6 dpf|sex:pooled male and female|tissue:whole head|BioSampleModel:Model organism or animal,,,,,,,,,Transcriptional profiling of hypocretin neurons identifies the sleep regulator Kcnh4a,WT 4,WT 4,Biological replicate 4,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,SRP058038,,loader:latf load,Sample_i-na_1.fastq Sample_i-na_2.fastq,fastq fastq,2622392600.0,26223926.0,WT 4,0:100,A:713649918;C:606120888;G:578652870;T:712333113;N:11635811,100,,,,713649918,606120888,578652870,712333113,11635811,SRX1030036,SRS938363,SRA266465,Bar Ilan University|Dr. Lior Appelbaum,Bar Ilan University,1,0.93312,,0.09652,,0.64758,,0.47024,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Israel,2016-05-06,Larval,Larval,Head,Nervous System 39643,SRR2025020,SRX1030035,SRS938363,SRP058038,PRJNA283169,Danio rerio Raw sequence reads,PRJNA283169,Other,RNA sequencing of whole head and Hcrt cells of 6dpf larvae zebrafish.,,,RNA was purified from WT 6dpf zebrafish larvae,WT whole head larva zebrafish,WT,,breed:AB|dev stage:6 dpf|sex:pooled male and female|tissue:whole head|BioSampleModel:Model organism or animal,,,,,,,,,Transcriptional profiling of hypocretin neurons identifies the sleep regulator Kcnh4a,WT 3,WT 3,Biological replicate 3,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,SRP058038,,loader:latf load,Sample_iii-a_2.fastq Sample_iii-a_1.fastq,fastq fastq,2578775300.0,25787753.0,WT 3,0:100,A:710064453;C:580284486;G:570277437;T:702861739;N:15287185,100,,,,710064453,580284486,570277437,702861739,15287185,SRX1030035,SRS938363,SRA266465,Bar Ilan University|Dr. Lior Appelbaum,Bar Ilan University,1,0.92355,,0.11281,,0.64346,,0.47868,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Israel,2016-05-06,Larval,Larval,Head,Nervous System 39644,SRR2025004,SRX1030034,SRS938363,SRP058038,PRJNA283169,Danio rerio Raw sequence reads,PRJNA283169,Other,RNA sequencing of whole head and Hcrt cells of 6dpf larvae zebrafish.,,,RNA was purified from WT 6dpf zebrafish larvae,WT whole head larva zebrafish,WT,,breed:AB|dev stage:6 dpf|sex:pooled male and female|tissue:whole head|BioSampleModel:Model organism or animal,,,,,,,,,Transcriptional profiling of hypocretin neurons identifies the sleep regulator Kcnh4a,WT 2,WT 2,Biological replicate 2,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,SRP058038,,loader:latf load,,,5748638600.0,57486386.0,WT 2,0:100,A:1582673281;C:1315049990;G:1252719550;T:1572983229;N:25212550,100,,,,1582673281,1315049990,1252719550,1572983229,25212550,SRX1030034,SRS938363,SRA266465,Bar Ilan University|Dr. Lior Appelbaum,Bar Ilan University,1,0.93088,,0.09912,,0.64439,,0.4756,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Israel,2015-05-18,Larval,Larval,Head,Nervous System 39645,SRR2025001,SRX1030032,SRS938363,SRP058038,PRJNA283169,Danio rerio Raw sequence reads,PRJNA283169,Other,RNA sequencing of whole head and Hcrt cells of 6dpf larvae zebrafish.,,,RNA was purified from WT 6dpf zebrafish larvae,WT whole head larva zebrafish,WT,,breed:AB|dev stage:6 dpf|sex:pooled male and female|tissue:whole head|BioSampleModel:Model organism or animal,,,,,,,,,Transcriptional profiling of hypocretin neurons identifies the sleep regulator Kcnh4a,WT 1,WT 1,RNA was purified from 6dpf WT larvae zebrafish. Biological replicate 1,,,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina HiSeq 2000,2000Application ReadForward11Application ReadReverse101,SRP058038,,loader:latf load,,,2116790100.0,21167901.0,WT 1,0:100,A:574102889;C:484943445;G:475977813;T:569490955;N:12274998,100,,,,574102889,484943445,475977813,569490955,12274998,SRX1030032,SRS938363,SRA266465,Bar Ilan University|Dr. Lior Appelbaum,Bar Ilan University,1,0.93025,,0.10052,,0.64398,,0.47695,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Israel,2015-05-18,Larval,Larval,Head,Nervous System