rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 49815,SRR8987978,SRX5767074,SRS4701368,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,24h 6,sample exposed to microcystin for xxxh replicate #6,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #6,24h 3,24h 3,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,24h3_clean_R2.fq.gz 24h3_clean_R1.fq.gz,fastq fastq,6275458528.0,20779664.0,24h3 clean R1.fq.gz,0:151 1:151,A:1685303886;C:1437291030;G:1447147160;T:1702856463;N:2859989,151,151,,,1685303886,1437291030,1447147160,1702856463,2859989,SRX5767074,SRS4701368,SRA880742,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92675,0.92679,0.13415,0.13431,0.73945,0.74213,0.50477,0.50726,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49816,SRR8987979,SRX5767073,SRS4701367,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,24h 4,sample exposed to microcystin for xxxh replicate #4,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #4,24h 1,24h 1,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,24h1_clean_R1.fq.gz 24h1_clean_R2.fq.gz,fastq fastq,6373552356.0,21104478.0,24h1 clean R1.fq.gz,0:151 1:151,A:1712827186;C:1458408871;G:1470508604;T:1728973011;N:2834684,151,151,,,1712827186,1458408871,1470508604,1728973011,2834684,SRX5767073,SRS4701367,SRA880742,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.9248,0.92582,0.13207,0.13239,0.73839,0.74038,0.49865,0.50119,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49817,SRR8987980,SRX5767072,SRS4701366,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,24h 5,sample exposed to microcystin for xxxh replicate #5,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #5,24h 2,24h 2,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,24h2_clean_R2.fq.gz 24h2_clean_R1.fq.gz,fastq fastq,6303822066.0,20873583.0,24h2 clean R1.fq.gz,0:151 1:151,A:1694366688;C:1441733171;G:1453717629;T:1711017538;N:2987040,151,151,,,1694366688,1441733171,1453717629,1711017538,2987040,SRX5767072,SRS4701366,SRA880742,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.9264,0.92591,0.13121,0.13204,0.7385,0.74227,0.50323,0.49357,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49818,SRR8983315,SRX5762615,SRS4697269,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,12h 5,sample exposed to microcystin for xxxh replicate #5,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #5,12h 5,12h 5,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,12h2_clean_R1.fq.gz 12h2_clean_R2.fq.gz,fastq fastq,5637564766.0,18667433.0,12h2 clean R1.fq.gz,0:151 1:151,A:1484457309;C:1319928521;G:1331480348;T:1498547286;N:3151302,151,151,,,1484457309,1319928521,1331480348,1498547286,3151302,SRX5762615,SRS4697269,SRA880316,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93974,0.93913,0.09518,0.09588,0.74982,0.75331,0.49858,0.49712,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49819,SRR8983316,SRX5762614,SRS4697268,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,12h 4,sample exposed to microcystin for xxxh replicate #4,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #4,12h 4,12h 4,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,12h1_clean_R2.fq.gz 12h1_clean_R1.fq.gz,fastq fastq,5742037438.0,19013369.0,12h1 clean R1.fq.gz,0:151 1:151,A:1505967171;C:1350499061;G:1361100937;T:1521258216;N:3212053,151,151,,,1505967171,1350499061,1361100937,1521258216,3212053,SRX5762614,SRS4697268,SRA880316,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.9408,0.93974,0.09145,0.09155,0.75146,0.75501,0.49821,0.49267,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49820,SRR8983317,SRX5762613,SRS4697267,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,12h 6,sample exposed to microcystin for xxxh replicate #6,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #6,12h 6,12h 6,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,12h3_clean_R2.fq.gz 12h3_clean_R1.fq.gz,fastq fastq,5456135246.0,18066673.0,12h3 clean R1.fq.gz,0:151 1:151,A:1445780220;C:1268483624;G:1278423072;T:1460394475;N:3053855,151,151,,,1445780220,1268483624,1278423072,1460394475,3053855,SRX5762613,SRS4697267,SRA880316,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93632,0.93624,0.10093,0.1015,0.7499,0.75367,0.50485,0.50164,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49821,SRR8982954,SRX5762254,SRS4696966,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,6h 6,sample exposed to microcystin for xxxh replicate #6,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #6,6h 6,6h 6,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,6h3_clean_R1.fq.gz 6h3_clean_R2.fq.gz,fastq fastq,7939056332.0,26288266.0,6h3 clean R1.fq.gz,0:151 1:151,A:2043932346;C:1908038526;G:1925388013;T:2058202469;N:3494978,151,151,,,2043932346,1908038526,1925388013,2058202469,3494978,SRX5762254,SRS4696966,SRA880287,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.94661,0.94661,0.07559,0.07638,0.76134,0.76394,0.49736,0.50336,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49822,SRR8982955,SRX5762253,SRS4696965,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,6h 5,sample exposed to microcystin for xxxh replicate #5,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #5,6h 5,6h 5,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,6h2_clean_R1.fq.gz 6h2_clean_R2.fq.gz,fastq fastq,5431283968.0,17984384.0,6h2 clean R1.fq.gz,0:151 1:151,A:1432960105;C:1269588003;G:1284040697;T:1441657813;N:3037350,151,151,,,1432960105,1269588003,1284040697,1441657813,3037350,SRX5762253,SRS4696965,SRA880287,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.9325,0.93223,0.11537,0.11461,0.7433,0.7457,0.49688,0.50558,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49823,SRR8982956,SRX5762252,SRS4696964,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,6h 4,sample exposed to microcystin for xxxh replicate #4,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #4,6h 4,6h 4,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,6h1_clean_R1.fq.gz 6h1_clean_R2.fq.gz,fastq fastq,5561923128.0,18416964.0,6h1 clean R1.fq.gz,0:151 1:151,A:1439698401;C:1327963901;G:1340151064;T:1450995064;N:3114698,151,151,,,1439698401,1327963901,1340151064,1450995064,3114698,SRX5762252,SRS4696964,SRA880287,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.9438,0.94314,0.08661,0.08624,0.75613,0.75858,0.49468,0.47945,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-29,Undetermined,Undetermined,Cell Line,Cell Line 49824,SRR8981191,SRX5760491,SRS4695477,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,3h 5,sample exposed to microcystin for xxxh replicate #5,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #5,3h 5,3h 5,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,3h2_clean_R1.fq.gz 3h2_clean_R2.fq.gz,fastq fastq,5426694474.0,17969187.0,3h2 clean R1.fq.gz,0:151 1:151,A:1455679038;C:1243463586;G:1253806438;T:1470703342;N:3042070,151,151,,,1455679038,1243463586,1253806438,1470703342,3042070,SRX5760491,SRS4695477,SRA880231,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92775,0.9265,0.13003,0.13039,0.74257,0.74679,0.48728,0.50236,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-28,Undetermined,Undetermined,Cell Line,Cell Line 49825,SRR8981192,SRX5760490,SRS4695476,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,3h 4,sample exposed to microcystin for xxxh replicate #4,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #4,3h 4,3h 4,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,3h1_clean_R1.fq.gz 3h1_clean_R2.fq.gz,fastq fastq,6381408584.0,21130492.0,3h1 clean R1.fq.gz,0:151 1:151,A:1704493392;C:1471437290;G:1482728107;T:1720014953;N:2734842,151,151,,,1704493392,1471437290,1482728107,1720014953,2734842,SRX5760490,SRS4695476,SRA880231,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93,0.93014,0.11977,0.12069,0.74434,0.74864,0.49627,0.50428,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-28,Undetermined,Undetermined,Cell Line,Cell Line 49826,SRR8981193,SRX5760489,SRS4695475,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,3h 6,sample exposed to microcystin for xxxh replicate #6,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #6,3h 6,3h 6,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,3h3_clean_R1.fq.gz 3h3_clean_R2.fq.gz,fastq fastq,6058080740.0,20059870.0,3h3 clean R1.fq.gz,0:151 1:151,A:1623401423;C:1391994584;G:1400166489;T:1639132542;N:3385702,151,151,,,1623401423,1391994584,1400166489,1639132542,3385702,SRX5760489,SRS4695475,SRA880231,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93298,0.93201,0.10907,0.10885,0.75059,0.75463,0.50656,0.49273,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-28,Undetermined,Undetermined,Cell Line,Cell Line 49827,SRR8961086,SRX5740640,SRS4676161,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,1h 6,sample exposed to microcystin for xxxh replicate #6,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #6,1h 6,1h 6,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,1h3_clean_R1.fq.gz 1h3_clean_R2.fq.gz,fastq fastq,6472187368.0,21431084.0,1h3 clean R1.fq.gz,0:151 1:151,A:1780621052;C:1438221849;G:1453983421;T:1796459482;N:2901564,151,151,,,1780621052,1438221849,1453983421,1796459482,2901564,SRX5740640,SRS4676161,SRA879791,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92158,0.91814,0.14503,0.14358,0.75775,0.75982,0.50094,0.49955,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-26,Undetermined,Undetermined,Cell Line,Cell Line 49828,SRR8961087,SRX5740639,SRS4676160,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,1h 4,sample exposed to microcystin for xxxh replicate #4,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #4,1h 4,1h 4,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,1h1_clean_R1.fq.gz 1h1_clean_R2.fq.gz,fastq fastq,6177620796.0,20455698.0,1h1 clean R1.fq.gz,0:151 1:151,A:1646973061;C:1422549043;G:1441385824;T:1663912518;N:2800350,151,151,,,1646973061,1422549043,1441385824,1663912518,2800350,SRX5740639,SRS4676160,SRA879791,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93085,0.92866,0.12633,0.12579,0.75812,0.76136,0.48992,0.50959,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-26,Undetermined,Undetermined,Cell Line,Cell Line 49829,SRR8961088,SRX5740638,SRS4676159,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,1h 5,sample exposed to microcystin for xxxh replicate #5,,ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxxh replicate #5,1h 5,1h 5,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,1h2_clean_R2.fq.gz 1h2_clean_R1.fq.gz,fastq fastq,5616651266.0,18598183.0,1h2 clean R1.fq.gz,0:151 1:151,A:1492544948;C:1296049637;G:1317613246;T:1507292547;N:3150888,151,151,,,1492544948,1296049637,1317613246,1507292547,3150888,SRX5740638,SRS4676159,SRA879791,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93168,0.93082,0.12771,0.12751,0.75653,0.75964,0.508,0.51051,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-26,Undetermined,Undetermined,Cell Line,Cell Line 49830,SRR8959882,SRX5739436,SRS4675912,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,control replicate #6,ck 6,,replicate:replicate=#6|ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|BioSampleModel:Model organism or animal,,,,,,,,,replicate 6,ck 6,ck 6,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,CK3_clean_R1.fq.gz CK3_clean_R2.fq.gz,fastq fastq,6417346282.0,21249491.0,CK3 clean R1.fq.gz,0:151 1:151,A:1753073270;C:1445910840;G:1452693305;T:1762084157;N:3584710,151,151,,,1753073270,1445910840,1452693305,1762084157,3584710,SRX5739436,SRS4675912,SRA879767,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92653,0.92662,0.11696,0.11682,0.75166,0.75379,0.50678,0.50594,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-26,Undetermined,Undetermined,Cell Line,Cell Line 49831,SRR8959883,SRX5739435,SRS4675911,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,control replicate #4,ck 4,,replicate:replicate=#4|ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|BioSampleModel:Model organism or animal,,,,,,,,,replicate 4,ck 4,ck 4,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,CK1_clean_R1.fq.gz CK1_clean_R2.fq.gz,fastq fastq,5659831830.0,18741165.0,CK1 clean R1.fq.gz,0:151 1:151,A:1532952307;C:1289729700;G:1295220307;T:1538761250;N:3168266,151,151,,,1532952307,1289729700,1295220307,1538761250,3168266,SRX5739435,SRS4675911,SRA879767,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92518,0.92535,0.11332,0.11378,0.74919,0.75235,0.50223,0.49803,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-26,Undetermined,Undetermined,Cell Line,Cell Line 49832,SRR8959884,SRX5739434,SRS4675910,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,control replicate #5,ck 5,,replicate:replicate=#5|ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|BioSampleModel:Model organism or animal,,,,,,,,,replicate 5,ck 5,ck 5,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,CK2_clean_R1.fq.gz CK2_clean_R2.fq.gz,fastq fastq,5880727616.0,19472608.0,CK2 clean R1.fq.gz,0:151 1:151,A:1575999691;C:1352057289;G:1366253556;T:1583120335;N:3296745,151,151,,,1575999691,1352057289,1366253556,1583120335,3296745,SRX5739434,SRS4675910,SRA879767,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93653,0.9349,0.09037,0.09026,0.76262,0.76572,0.51143,0.50376,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-04-26,Undetermined,Undetermined,Cell Line,Cell Line 49833,SRR8133154,SRX4954244,SRS3995636,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #1,24h 1,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #1,24h 1,24h 1,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,24h-1_R1.fastq.gz 24h-1_R2.fastq.gz,fastq fastq,6408674352.0,21220776.0,24h 1 R1.fastq.gz,0:151 1:151,A:1752104547;C:1447174976;G:1451968217;T:1757298923;N:127689,151,151,,,1752104547,1447174976,1451968217,1757298923,127689,SRX4954244,SRS3995636,SRA800477,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.9212,0.91969,0.14419,0.14446,0.73677,0.74363,0.49228,0.49725,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2018-10-30,Undetermined,Undetermined,Cell Line,Cell Line 49834,SRR8133155,SRX4954243,SRS3995635,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #2,24h 2,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #2,24h 2,24h 2,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,24h-2_R1.fastq.gz 24h-2_R2.fastq.gz,fastq fastq,6331528754.0,20965327.0,24h 2 R1.fastq.gz,0:151 1:151,A:1725083256;C:1436372645;G:1442486884;T:1727457912;N:128057,151,151,,,1725083256,1436372645,1442486884,1727457912,128057,SRX4954243,SRS3995635,SRA800477,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92281,0.92303,0.13905,0.14045,0.73708,0.74401,0.50843,0.50295,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49835,SRR8133156,SRX4954242,SRS3995634,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #3,24h 3,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #3,24h 3,24h 3,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,24h-3_R1.fastq.gz 24h-3_R2.fastq.gz,fastq fastq,6723114940.0,22261970.0,24h 3 R1.fastq.gz,0:151 1:151,A:1833689339;C:1522824611;G:1529534136;T:1836931192;N:135662,151,151,,,1833689339,1522824611,1529534136,1836931192,135662,SRX4954242,SRS3995634,SRA800477,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.91356,0.91347,0.13955,0.14059,0.73898,0.74399,0.49776,0.50087,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49836,SRR8133151,SRX4954241,SRS3995633,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #3,12h 3,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #3,12h 3,12h 3,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,12h-3_R1.fastq.gz 12h-3_R2.fastq.gz,fastq fastq,6249709404.0,20694402.0,12h 3 R1.fastq.gz,0:151 1:151,A:1679876276;C:1439210018;G:1449953029;T:1680544537;N:125544,151,151,,,1679876276,1439210018,1449953029,1680544537,125544,SRX4954241,SRS3995633,SRA800476,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.9318,0.93185,0.10315,0.10397,0.7499,0.75513,0.49669,0.50516,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49837,SRR8133152,SRX4954240,SRS3995632,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #1,12h 1,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #1,12h 1,12h 1,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,12h-1_R1.fastq.gz 12h-1_R2.fastq.gz,fastq fastq,6040713928.0,20002364.0,12h 1 R1.fastq.gz,0:151 1:151,A:1604304459;C:1407301760;G:1430569355;T:1597934265;N:604089,151,151,,,1604304459,1407301760,1430569355,1597934265,604089,SRX4954240,SRS3995632,SRA800476,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93665,0.93821,0.09301,0.0957,0.75284,0.77169,0.49115,0.48606,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49838,SRR8133153,SRX4954239,SRS3995631,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #2,12h 2,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #2,12h 2,12h 2,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,12h-2_R1.fastq.gz 12h-2_R2.fastq.gz,fastq fastq,6340973200.0,20996600.0,12h 2 R1.fastq.gz,0:151 1:151,A:1704338617;C:1461869913;G:1470728810;T:1703906206;N:129654,151,151,,,1704338617,1461869913,1470728810,1703906206,129654,SRX4954239,SRS3995631,SRA800476,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93272,0.93343,0.10216,0.10338,0.74978,0.75513,0.50094,0.51045,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49839,SRR8132757,SRX4953863,SRS3995402,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #3,6h 3,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #3,6h 3,6h 3,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,6h-3_R1.fastq.gz 6h-3_R2.fastq.gz,fastq fastq,6533368340.0,21633670.0,6h 3 R1.fastq.gz,0:151 1:151,A:1684379253;C:1575728468;G:1593737205;T:1678875387;N:648027,151,151,,,1684379253,1575728468,1593737205,1678875387,648027,SRX4953863,SRS3995402,SRA800451,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.94744,0.94959,0.06681,0.06774,0.76459,0.77711,0.4873,0.49779,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49840,SRR8132756,SRX4953862,SRS3995403,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #1,6h 1,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #1,6h 1,6h 1,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,6h-1_R1.fastq.gz 6h-1_R2.fastq.gz,fastq fastq,6686156482.0,22139591.0,6h 1 R1.fastq.gz,0:151 1:151,A:1712757343;C:1622803789;G:1646819107;T:1703094369;N:681874,151,151,,,1712757343,1622803789,1646819107,1703094369,681874,SRX4953862,SRS3995403,SRA800449,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.95018,0.95234,0.0661,0.06774,0.77268,0.78652,0.49287,0.48663,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2018-10-29,Undetermined,Undetermined,Cell Line,Cell Line 49841,SRR8132755,SRX4953861,SRS3995401,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #2,6h 2,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #2,6h 2,6h 2,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,6h-2_R1.fastq.gz 6h-2_R2.fastq.gz,fastq fastq,5551304204.0,18381802.0,6h 2 R1.fastq.gz,0:151 1:151,A:1408740987;C:1361721973;G:1380328727;T:1399965481;N:547036,151,151,,,1408740987,1361721973,1380328727,1399965481,547036,SRX4953861,SRS3995401,SRA800450,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.9506,0.95313,0.07031,0.07155,0.7739,0.78591,0.50125,0.49851,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2018-10-29,Undetermined,Undetermined,Cell Line,Cell Line 49842,SRR8119911,SRX4946208,SRS3988532,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #3,3h 3,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #3,3h 3,3h 3,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,3h-3_R1.fastq.gz 3h-3_R2.fastq.gz,fastq fastq,6249422202.0,20693451.0,3h 3 R1.fastq.gz,0:151 1:151,A:1700610088;C:1416827975;G:1436930329;T:1694433965;N:619845,151,151,,,1700610088,1416827975,1436930329,1694433965,619845,SRX4946208,SRS3988532,SRA799983,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.924,0.92633,0.13412,0.13864,0.74158,0.76489,0.49551,0.49903,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2018-10-29,Undetermined,Undetermined,Cell Line,Cell Line 49843,SRR8119910,SRX4946207,SRS3988531,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #2,3h 2,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #2,3h 2,3h 2,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,3h-2_R1.fastq.gz 3h-2_R2.fastq.gz,fastq fastq,6691460508.0,22157154.0,3h 2 R2.fastq.gz,0:151 1:151,A:1817827404;C:1520902100;G:1534921644;T:1817122657;N:686703,151,151,,,1817827404,1520902100,1534921644,1817122657,686703,SRX4946207,SRS3988531,SRA799982,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92541,0.92734,0.13003,0.1321,0.7443,0.76019,0.50143,0.4998,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49844,SRR8117643,SRX4943940,SRS3986328,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #1,3h 1,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #1,3h 1,3h 1,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,3h-1_R1.fastq.gz 3h-1_R2.fastq.gz,fastq fastq,6151798890.0,20370195.0,3h 1 R1.fastq.gz,0:151 1:151,A:1649142984;C:1419068750;G:1435991843;T:1646989815;N:605498,151,151,,,1649142984,1419068750,1435991843,1646989815,605498,SRX4943940,SRS3986328,SRA799824,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92965,0.93287,0.12221,0.12677,0.73397,0.75278,0.50535,0.50378,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2018-10-28,Undetermined,Undetermined,Cell Line,Cell Line 49845,SRR8115441,SRX4941738,SRS3985539,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #3,1h 3,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #3,1h 3,1h 3,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,1h-3_R2.fastq.gz 1h-3_R1.fastq.gz,fastq fastq,7655572254.0,25349577.0,1h 3 R2.fastq.gz,0:151 1:151,A:2078212614;C:1742053699;G:1754520045;T:2080018836;N:767060,151,151,,,2078212614,1742053699,1754520045,2080018836,767060,SRX4941738,SRS3985539,SRA799651,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92526,0.92744,0.12977,0.13298,0.75743,0.77061,0.50353,0.50471,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49846,SRR8115440,SRX4941737,SRS3985538,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #2,1h 2,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #2,1h 2,1h 2,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,1h-2_R1.fastq.gz 1h-2_R2.fastq.gz,fastq fastq,7594153306.0,25146203.0,1h 2 R1.fastq.gz,0:151 1:151,A:2048228795;C:1739666332;G:1763496812;T:2041988124;N:773243,151,151,,,2048228795,1739666332,1763496812,2041988124,773243,SRX4941737,SRS3985538,SRA799650,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92922,0.93046,0.12988,0.1333,0.75657,0.77684,0.50439,0.49684,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line 49847,SRR8109598,SRX4936168,SRS3980334,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample exposed to microcystin for xxx hour replicate #1,1h 1,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal,,,,,,,,,sample exposed to microcystin for xxx hour replicate #1,1h 1,1h 1,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,1h-1_R1.fastq.gz 1h-1_R2.fastq.gz,fastq fastq,6505465654.0,21541277.0,1h 1 R2.fastq.gz,0:151 1:151,A:1772064909;C:1473729340;G:1487273483;T:1771747419;N:650503,151,151,,,1772064909,1473729340,1487273483,1771747419,650503,SRX4936168,SRS3980334,SRA798650,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.92342,0.92707,0.13549,0.13873,0.7555,0.77106,0.49839,0.50202,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2018-10-25,Undetermined,Undetermined,Cell Line,Cell Line 49848,SRR8109308,SRX4935890,SRS3980091,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample not treated replicate #3,ck 3,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:not treated replicate #3|BioSampleModel:Model organism or animal,,,,,,,,,sample not treated replicate #3,ck 3,ck 3,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,CK-3_R1.fastq.gz CK-3_R2.fastq.gz,fastq fastq,6691086632.0,22155916.0,CK 3 R2.fastq.gz,0:151 1:151,A:1817102254;C:1525946318;G:1537285128;T:1810073033;N:679899,151,151,,,1817102254,1525946318,1537285128,1810073033,679899,SRX4935890,SRS3980091,SRA798632,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93213,0.93388,0.10099,0.10342,0.75828,0.77035,0.49928,0.49506,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2018-10-25,Undetermined,Undetermined,Cell Line,Cell Line 49849,SRR8109041,SRX4935630,SRS3979958,SRP166817,PRJNA498018,Danio rerio strain:ZFL cell line Genome sequencing,PRJNA498018,Whole Genome Sequencing,Transcriptional responses of ZFL exposed to microcystin LR,,,,sample not treated replicate #2,ck 2,,isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:not treated replicate #1|BioSampleModel:Model organism or animal,,,,,,,,,sample not treated replicate #2,ck 2,ck 2,Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,ILLUMINA,HiSeq X Ten,,SRP166817,,,CK-2_R2.fastq.gz CK-2_R1.fastq.gz,fastq fastq,6135368580.0,20315790.0,CK 2 R2.fastq.gz,0:151 1:151,A:1659190299;C:1402338038;G:1422425283;T:1650808795;N:606165,151,151,,,1659190299,1402338038,1422425283,1650808795,606165,SRX4935630,SRS3979958,SRA798622,Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,2,0.93276,0.93529,0.09768,0.1008,0.75905,0.77512,0.48616,0.50755,151,151,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,China,2019-01-01,Undetermined,Undetermined,Cell Line,Cell Line