rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 30738,SRR28346485,SRX23952998,SRS20753493,SRP495277,PRJNA1088100,Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo,GSE261604,Transcriptome Analysis,To identify the crosstalk between Wnt/ß catenin signaling activated cardiomyocytes ß cat ON CMs and coronary endothelial cells coECs we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/ß catenin signaling we also collected AVC from fish treated with DMSO or IWR1 endo an inhibitor of Wnt/ß catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between ß cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 µM IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.,parent bioproject:PRJNA926354,pubmed:39395410,,AVC IWR1 scRNAseq,GSM8147318,,source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1|geo loc name:missing|collection date:missing,AVC IWR1 scRNAseq,The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing alignment to the reference genome Danio rerio GRCz11 barcode/UMI processing and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample mtx files count gene UMI couns for each sample,Heart,Fish were treated with DMSO or 10 μM MTZ dissolved in fish water for 48 h in the dark.,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3’reagent kits v3.1 10X Genomics 1000128 according to the manufacturer’s protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1,GSM8147318,GSM8147318: AVC IWR1 scRNAseq; Danio rerio; RNA Seq,GSM8147318 r1,GSM8147318,1,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics 1000128 according to the manufacturer's protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP495277,,loader:fastq load.py,AVC_IWR1_S1_L001_I1_001.fastq.gz AVC_IWR1_S1_L001_I2_001.fastq.gz AVC_IWR1_S1_L001_R1_001.fastq.gz AVC_IWR1_S1_L001_R2_001.fastq.gz,fastq fastq fastq fastq,8596842480.0,62295960.0,GSM8147318 r1,0:10 1:10 2:28 3:90,A:1570400090;C:1308581548;G:1326249813;T:1399966642;N:1438307,10,10,28,90,1570400090,1308581548,1326249813,1399966642,1438307,SRX23952998,SRS20753493,SRA1824323,"Department of Pharmacology, Yamagata University","Department of Pharmacology, Yamagata University",,,,,,,,,,,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Japan,2024-03-14,Juvenile,Juvenile,Heart,Cardiovascular System 30739,SRR28346486,SRX23952998,SRS20753493,SRP495277,PRJNA1088100,Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo,GSE261604,Transcriptome Analysis,To identify the crosstalk between Wnt/ß catenin signaling activated cardiomyocytes ß cat ON CMs and coronary endothelial cells coECs we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/ß catenin signaling we also collected AVC from fish treated with DMSO or IWR1 endo an inhibitor of Wnt/ß catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between ß cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 µM IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.,parent bioproject:PRJNA926354,pubmed:39395410,,AVC IWR1 scRNAseq,GSM8147318,,source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1|geo loc name:missing|collection date:missing,AVC IWR1 scRNAseq,The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing alignment to the reference genome Danio rerio GRCz11 barcode/UMI processing and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample mtx files count gene UMI couns for each sample,Heart,Fish were treated with DMSO or 10 μM MTZ dissolved in fish water for 48 h in the dark.,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3’reagent kits v3.1 10X Genomics 1000128 according to the manufacturer’s protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1,GSM8147318,GSM8147318: AVC IWR1 scRNAseq; Danio rerio; RNA Seq,GSM8147318 r1,GSM8147318,1,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics 1000128 according to the manufacturer's protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP495277,,loader:fastq load.py,AVC_IWR1_S1_L002_I1_001.fastq.gz AVC_IWR1_S1_L002_I2_001.fastq.gz AVC_IWR1_S1_L002_R1_001.fastq.gz AVC_IWR1_S1_L002_R2_001.fastq.gz,fastq fastq fastq fastq,8505185502.0,61631779.0,GSM8147318 r2,0:10 1:10 2:28 3:90,A:1553616390;C:1294687778;G:1312612882;T:1384504011;N:1439049,10,10,28,90,1553616390,1294687778,1312612882,1384504011,1439049,SRX23952998,SRS20753493,SRA1824323,"Department of Pharmacology, Yamagata University","Department of Pharmacology, Yamagata University",,,,,,,,,,,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Japan,2024-03-14,Juvenile,Juvenile,Heart,Cardiovascular System 30740,SRR28346487,SRX23952998,SRS20753493,SRP495277,PRJNA1088100,Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo,GSE261604,Transcriptome Analysis,To identify the crosstalk between Wnt/ß catenin signaling activated cardiomyocytes ß cat ON CMs and coronary endothelial cells coECs we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/ß catenin signaling we also collected AVC from fish treated with DMSO or IWR1 endo an inhibitor of Wnt/ß catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between ß cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 µM IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.,parent bioproject:PRJNA926354,pubmed:39395410,,AVC IWR1 scRNAseq,GSM8147318,,source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1|geo loc name:missing|collection date:missing,AVC IWR1 scRNAseq,The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing alignment to the reference genome Danio rerio GRCz11 barcode/UMI processing and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample mtx files count gene UMI couns for each sample,Heart,Fish were treated with DMSO or 10 μM MTZ dissolved in fish water for 48 h in the dark.,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3’reagent kits v3.1 10X Genomics 1000128 according to the manufacturer’s protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1,GSM8147318,GSM8147318: AVC IWR1 scRNAseq; Danio rerio; RNA Seq,GSM8147318 r1,GSM8147318,1,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics 1000128 according to the manufacturer's protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP495277,,loader:fastq load.py,AVC_IWR1_S1_L003_I1_001.fastq.gz AVC_IWR1_S1_L003_I2_001.fastq.gz AVC_IWR1_S1_L003_R1_001.fastq.gz AVC_IWR1_S1_L003_R2_001.fastq.gz,fastq fastq fastq fastq,8699205498.0,63037721.0,GSM8147318 r3,0:10 1:10 2:28 3:90,A:1588465122;C:1324147204;G:1343996634;T:1415623074;N:1162856,10,10,28,90,1588465122,1324147204,1343996634,1415623074,1162856,SRX23952998,SRS20753493,SRA1824323,"Department of Pharmacology, Yamagata University","Department of Pharmacology, Yamagata University",,,,,,,,,,,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Japan,2024-03-14,Juvenile,Juvenile,Heart,Cardiovascular System 30741,SRR28346488,SRX23952998,SRS20753493,SRP495277,PRJNA1088100,Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo,GSE261604,Transcriptome Analysis,To identify the crosstalk between Wnt/ß catenin signaling activated cardiomyocytes ß cat ON CMs and coronary endothelial cells coECs we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/ß catenin signaling we also collected AVC from fish treated with DMSO or IWR1 endo an inhibitor of Wnt/ß catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between ß cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 µM IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.,parent bioproject:PRJNA926354,pubmed:39395410,,AVC IWR1 scRNAseq,GSM8147318,,source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1|geo loc name:missing|collection date:missing,AVC IWR1 scRNAseq,The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing alignment to the reference genome Danio rerio GRCz11 barcode/UMI processing and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample mtx files count gene UMI couns for each sample,Heart,Fish were treated with DMSO or 10 μM MTZ dissolved in fish water for 48 h in the dark.,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3’reagent kits v3.1 10X Genomics 1000128 according to the manufacturer’s protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1,GSM8147318,GSM8147318: AVC IWR1 scRNAseq; Danio rerio; RNA Seq,GSM8147318 r1,GSM8147318,1,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics 1000128 according to the manufacturer's protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP495277,,loader:fastq load.py,AVC_IWR1_S1_L004_I1_001.fastq.gz AVC_IWR1_S1_L004_I2_001.fastq.gz AVC_IWR1_S1_L004_R1_001.fastq.gz AVC_IWR1_S1_L004_R2_001.fastq.gz,fastq fastq fastq fastq,8627728812.0,62519774.0,GSM8147318 r4,0:10 1:10 2:28 3:90,A:1576009383;C:1313466786;G:1332114241;T:1404392019;N:797231,10,10,28,90,1576009383,1313466786,1332114241,1404392019,797231,SRX23952998,SRS20753493,SRA1824323,"Department of Pharmacology, Yamagata University","Department of Pharmacology, Yamagata University",,,,,,,,,,,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Japan,2024-03-14,Juvenile,Juvenile,Heart,Cardiovascular System 30742,SRR28346489,SRX23952997,SRS20753492,SRP495277,PRJNA1088100,Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo,GSE261604,Transcriptome Analysis,To identify the crosstalk between Wnt/ß catenin signaling activated cardiomyocytes ß cat ON CMs and coronary endothelial cells coECs we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/ß catenin signaling we also collected AVC from fish treated with DMSO or IWR1 endo an inhibitor of Wnt/ß catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between ß cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 µM IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.,parent bioproject:PRJNA926354,pubmed:39395410,,AVC DMSO scRNAseq,GSM8147317,,source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO|geo loc name:missing|collection date:missing,AVC DMSO scRNAseq,The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing alignment to the reference genome Danio rerio GRCz11 barcode/UMI processing and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample mtx files count gene UMI couns for each sample,Heart,Fish were treated with DMSO or 10 μM MTZ dissolved in fish water for 48 h in the dark.,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3’reagent kits v3.1 10X Genomics 1000128 according to the manufacturer’s protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO,GSM8147317,GSM8147317: AVC DMSO scRNAseq; Danio rerio; RNA Seq,GSM8147317 r1,GSM8147317,1,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics 1000128 according to the manufacturer's protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP495277,,loader:fastq load.py,AVC_Ctrl_S1_L001_I1_001.fastq.gz AVC_Ctrl_S1_L001_I2_001.fastq.gz AVC_Ctrl_S1_L001_R1_001.fastq.gz AVC_Ctrl_S1_L001_R2_001.fastq.gz,fastq fastq fastq fastq,7385103810.0,53515245.0,GSM8147317 r1,0:10 1:10 2:28 3:90,A:1329371520;C:1135299252;G:1143250549;T:1207226310;N:1224419,10,10,28,90,1329371520,1135299252,1143250549,1207226310,1224419,SRX23952997,SRS20753492,SRA1824323,"Department of Pharmacology, Yamagata University","Department of Pharmacology, Yamagata University",,,,,,,,,,,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Japan,2024-03-14,Juvenile,Juvenile,Heart,Cardiovascular System 30743,SRR28346490,SRX23952997,SRS20753492,SRP495277,PRJNA1088100,Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo,GSE261604,Transcriptome Analysis,To identify the crosstalk between Wnt/ß catenin signaling activated cardiomyocytes ß cat ON CMs and coronary endothelial cells coECs we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/ß catenin signaling we also collected AVC from fish treated with DMSO or IWR1 endo an inhibitor of Wnt/ß catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between ß cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 µM IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.,parent bioproject:PRJNA926354,pubmed:39395410,,AVC DMSO scRNAseq,GSM8147317,,source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO|geo loc name:missing|collection date:missing,AVC DMSO scRNAseq,The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing alignment to the reference genome Danio rerio GRCz11 barcode/UMI processing and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample mtx files count gene UMI couns for each sample,Heart,Fish were treated with DMSO or 10 μM MTZ dissolved in fish water for 48 h in the dark.,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3’reagent kits v3.1 10X Genomics 1000128 according to the manufacturer’s protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO,GSM8147317,GSM8147317: AVC DMSO scRNAseq; Danio rerio; RNA Seq,GSM8147317 r1,GSM8147317,1,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics 1000128 according to the manufacturer's protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP495277,,loader:fastq load.py,AVC_Ctrl_S1_L002_I1_001.fastq.gz AVC_Ctrl_S1_L002_I2_001.fastq.gz AVC_Ctrl_S1_L002_R1_001.fastq.gz AVC_Ctrl_S1_L002_R2_001.fastq.gz,fastq fastq fastq fastq,7313299098.0,52994921.0,GSM8147317 r2,0:10 1:10 2:28 3:90,A:1316101332;C:1124200896;G:1133146934;T:1194866024;N:1227704,10,10,28,90,1316101332,1124200896,1133146934,1194866024,1227704,SRX23952997,SRS20753492,SRA1824323,"Department of Pharmacology, Yamagata University","Department of Pharmacology, Yamagata University",,,,,,,,,,,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Japan,2024-03-14,Juvenile,Juvenile,Heart,Cardiovascular System 30744,SRR28346491,SRX23952997,SRS20753492,SRP495277,PRJNA1088100,Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo,GSE261604,Transcriptome Analysis,To identify the crosstalk between Wnt/ß catenin signaling activated cardiomyocytes ß cat ON CMs and coronary endothelial cells coECs we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/ß catenin signaling we also collected AVC from fish treated with DMSO or IWR1 endo an inhibitor of Wnt/ß catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between ß cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 µM IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.,parent bioproject:PRJNA926354,pubmed:39395410,,AVC DMSO scRNAseq,GSM8147317,,source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO|geo loc name:missing|collection date:missing,AVC DMSO scRNAseq,The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing alignment to the reference genome Danio rerio GRCz11 barcode/UMI processing and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample mtx files count gene UMI couns for each sample,Heart,Fish were treated with DMSO or 10 μM MTZ dissolved in fish water for 48 h in the dark.,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3’reagent kits v3.1 10X Genomics 1000128 according to the manufacturer’s protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO,GSM8147317,GSM8147317: AVC DMSO scRNAseq; Danio rerio; RNA Seq,GSM8147317 r1,GSM8147317,1,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics 1000128 according to the manufacturer's protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP495277,,loader:fastq load.py,AVC_Ctrl_S1_L003_I1_001.fastq.gz AVC_Ctrl_S1_L003_I2_001.fastq.gz AVC_Ctrl_S1_L003_R1_001.fastq.gz AVC_Ctrl_S1_L003_R2_001.fastq.gz,fastq fastq fastq fastq,7457857272.0,54042444.0,GSM8147317 r3,0:10 1:10 2:28 3:90,A:1341763332;C:1146520201;G:1156131488;T:1218395804;N:1009135,10,10,28,90,1341763332,1146520201,1156131488,1218395804,1009135,SRX23952997,SRS20753492,SRA1824323,"Department of Pharmacology, Yamagata University","Department of Pharmacology, Yamagata University",,,,,,,,,,,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Japan,2024-03-14,Juvenile,Juvenile,Heart,Cardiovascular System 30745,SRR28346492,SRX23952997,SRS20753492,SRP495277,PRJNA1088100,Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo,GSE261604,Transcriptome Analysis,To identify the crosstalk between Wnt/ß catenin signaling activated cardiomyocytes ß cat ON CMs and coronary endothelial cells coECs we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/ß catenin signaling we also collected AVC from fish treated with DMSO or IWR1 endo an inhibitor of Wnt/ß catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between ß cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 µM IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.,parent bioproject:PRJNA926354,pubmed:39395410,,AVC DMSO scRNAseq,GSM8147317,,source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO|geo loc name:missing|collection date:missing,AVC DMSO scRNAseq,The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing alignment to the reference genome Danio rerio GRCz11 barcode/UMI processing and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample mtx files count gene UMI couns for each sample,Heart,Fish were treated with DMSO or 10 μM MTZ dissolved in fish water for 48 h in the dark.,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3’reagent kits v3.1 10X Genomics 1000128 according to the manufacturer’s protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO,GSM8147317,GSM8147317: AVC DMSO scRNAseq; Danio rerio; RNA Seq,GSM8147317 r1,GSM8147317,1,Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCFΔC;5xUAS:NTR mCherry fish treated with DMSO or 10 μM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington LS004188/HBSS for 30 min at 28.5 ºC. post pipetting the cells were collected in low binding tubes pelleted 3000 rpm 5 min at 4ºC and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics 1000128 according to the manufacturer's protocol. Following droplet generation and barcoding cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 500,,SRP495277,,loader:fastq load.py,AVC_Ctrl_S1_L004_I1_001.fastq.gz AVC_Ctrl_S1_L004_I2_001.fastq.gz AVC_Ctrl_S1_L004_R1_001.fastq.gz AVC_Ctrl_S1_L004_R2_001.fastq.gz,fastq fastq fastq fastq,7430049030.0,53840935.0,GSM8147317 r4,0:10 1:10 2:28 3:90,A:1337094348;C:1142353903;G:1151539276;T:1214006711;N:689912,10,10,28,90,1337094348,1142353903,1151539276,1214006711,689912,SRX23952997,SRS20753492,SRA1824323,"Department of Pharmacology, Yamagata University","Department of Pharmacology, Yamagata University",,,,,,,,,,,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,Japan,2024-03-14,Juvenile,Juvenile,Heart,Cardiovascular System 71807,SRR22058034,SRX18039091,SRS15545647,SRP404721,PRJNA894591,hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev hapln1a+ cells],GSE216646,Other,As cardiac regeneration requires new coronary vessels exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation the interaction between coronary growth and guidance cues remains unclear. Here by applying single cell RNA sequencing scRNA seq analysis we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears with depletion of hapln1a+ cells blocking this growth. Further we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration by establishing a microenvironment to facilitate guided coronary growth. Overall design: hapln1a+ cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.,parent bioproject:PRJNA894685,pubmed:37311876,,hapln1a+ development scRNAseq,GSM6685234,,source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing,hapln1a+ development scRNAseq,All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual,heart,,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter’s instructions single cell 3’ v3.1 protocol 10x Genomics ,,strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:hapln1a+|age:7 weeks,GSM6685234,GSM6685234: hapln1a+ development scRNAseq; Danio rerio; RNA Seq,GSM6685234 r1,GSM6685234,1,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol 10x Genomics ,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 550,,SRP404721,,loader:fastq load.py,1_S1_L001_I1_001.fastq.gz 1_S1_L001_R1_001.fastq.gz 1_S1_L001_R2_001.fastq.gz,fastq fastq fastq,1606360897.0,12648511.0,GSM6685234 r1,0:8 1:28 2:91,A:339791226;C:243552181;G:266423271;T:301107277;N:140546,8,28,91,,339791226,243552181,266423271,301107277,140546,SRX18039091,SRS15545647,SRA1528482,Emory University,Emory University,1,0.92176,,0.1499,,0.81781,,0.50927,,91,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-10-26,Juvenile,Juvenile,Heart,Cardiovascular System 71808,SRR22058035,SRX18039091,SRS15545647,SRP404721,PRJNA894591,hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev hapln1a+ cells],GSE216646,Other,As cardiac regeneration requires new coronary vessels exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation the interaction between coronary growth and guidance cues remains unclear. Here by applying single cell RNA sequencing scRNA seq analysis we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears with depletion of hapln1a+ cells blocking this growth. Further we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration by establishing a microenvironment to facilitate guided coronary growth. Overall design: hapln1a+ cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.,parent bioproject:PRJNA894685,pubmed:37311876,,hapln1a+ development scRNAseq,GSM6685234,,source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing,hapln1a+ development scRNAseq,All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual,heart,,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter’s instructions single cell 3’ v3.1 protocol 10x Genomics ,,strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:hapln1a+|age:7 weeks,GSM6685234,GSM6685234: hapln1a+ development scRNAseq; Danio rerio; RNA Seq,GSM6685234 r1,GSM6685234,1,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol 10x Genomics ,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 550,,SRP404721,,loader:fastq load.py,1_S2_L001_I1_001.fastq.gz 1_S2_L001_R1_001.fastq.gz 1_S2_L001_R2_001.fastq.gz,fastq fastq fastq,1332426088.0,10491544.0,GSM6685234 r2,0:8 1:28 2:91,A:281795062;C:202126091;G:221122322;T:249574256;N:112773,8,28,91,,281795062,202126091,221122322,249574256,112773,SRX18039091,SRS15545647,SRA1528482,Emory University,Emory University,1,0.92141,,0.14909,,0.81714,,0.50543,,91,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-10-26,Juvenile,Juvenile,Heart,Cardiovascular System 71809,SRR22058036,SRX18039091,SRS15545647,SRP404721,PRJNA894591,hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev hapln1a+ cells],GSE216646,Other,As cardiac regeneration requires new coronary vessels exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation the interaction between coronary growth and guidance cues remains unclear. Here by applying single cell RNA sequencing scRNA seq analysis we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears with depletion of hapln1a+ cells blocking this growth. Further we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration by establishing a microenvironment to facilitate guided coronary growth. Overall design: hapln1a+ cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.,parent bioproject:PRJNA894685,pubmed:37311876,,hapln1a+ development scRNAseq,GSM6685234,,source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing,hapln1a+ development scRNAseq,All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual,heart,,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter’s instructions single cell 3’ v3.1 protocol 10x Genomics ,,strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:hapln1a+|age:7 weeks,GSM6685234,GSM6685234: hapln1a+ development scRNAseq; Danio rerio; RNA Seq,GSM6685234 r1,GSM6685234,1,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol 10x Genomics ,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 550,,SRP404721,,loader:fastq load.py,1_S3_L001_I1_001.fastq.gz 1_S3_L001_R1_001.fastq.gz 1_S3_L001_R2_001.fastq.gz,fastq fastq fastq,1187072175.0,9347025.0,GSM6685234 r3,0:8 1:28 2:91,A:251072030;C:180002392;G:196984629;T:222418470;N:101754,8,28,91,,251072030,180002392,196984629,222418470,101754,SRX18039091,SRS15545647,SRA1528482,Emory University,Emory University,1,0.92231,,0.15014,,0.8174,,0.50735,,91,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-10-26,Juvenile,Juvenile,Heart,Cardiovascular System 71810,SRR22058037,SRX18039091,SRS15545647,SRP404721,PRJNA894591,hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev hapln1a+ cells],GSE216646,Other,As cardiac regeneration requires new coronary vessels exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation the interaction between coronary growth and guidance cues remains unclear. Here by applying single cell RNA sequencing scRNA seq analysis we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears with depletion of hapln1a+ cells blocking this growth. Further we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration by establishing a microenvironment to facilitate guided coronary growth. Overall design: hapln1a+ cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.,parent bioproject:PRJNA894685,pubmed:37311876,,hapln1a+ development scRNAseq,GSM6685234,,source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing,hapln1a+ development scRNAseq,All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual,heart,,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter’s instructions single cell 3’ v3.1 protocol 10x Genomics ,,strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:hapln1a+|age:7 weeks,GSM6685234,GSM6685234: hapln1a+ development scRNAseq; Danio rerio; RNA Seq,GSM6685234 r1,GSM6685234,1,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol 10x Genomics ,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 550,,SRP404721,,loader:fastq load.py,1_S4_L001_I1_001.fastq.gz 1_S4_L001_R1_001.fastq.gz 1_S4_L001_R2_001.fastq.gz,fastq fastq fastq,1362894785.0,10731455.0,GSM6685234 r4,0:8 1:28 2:91,A:288451488;C:206540819;G:226008729;T:255442217;N:119152,8,28,91,,288451488,206540819,226008729,255442217,119152,SRX18039091,SRS15545647,SRA1528482,Emory University,Emory University,1,0.92215,,0.15108,,0.81706,,0.51589,,91,,B,,usable mapping rate,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-10-26,Juvenile,Juvenile,Heart,Cardiovascular System 71811,SRR22058440,SRX18039491,SRS15546048,SRP404726,PRJNA894687,hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev tcf21+ cells],GSE216648,Other,As cardiac regeneration requires new coronary vessels exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation the interaction between coronary growth and guidance cues remains unclear. Here by applying single cell RNA sequencing scRNA seq analysis we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears with depletion of hapln1a+ cells blocking this growth. Further we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration by establishing a microenvironment to facilitate guided coronary growth. Overall design: Epicardial cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.,parent bioproject:PRJNA894685,pubmed:37311876,,tcf21+ development scRNAseq,GSM6685266,,source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing,tcf21+ development scRNAseq,All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual,heart,,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter’s instructions single cell 3’ v3.1 protocol 10x Genomics ,,strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:tcf21+|age:7 weeks,GSM6685266,GSM6685266: tcf21+ development scRNAseq; Danio rerio; RNA Seq,GSM6685266 r1,GSM6685266,1,Hearts were dissected from juvenile zebrafish and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol 10x Genomics ,,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,cDNA,PAIRED,ILLUMINA,NextSeq 550,,SRP404726,,,TCF_21_S1_L001_R1_001.fastq.gz TCF_21_S1_L001_R2_001.fastq.gz,fastq fastq,19362384447.0,162709113.0,GSM6685266 r1,0:28 1:91,A:5391284818;C:4382019183;G:4643235756;T:4943804153;N:2040537,28,91,,,5391284818,4382019183,4643235756,4943804153,2040537,SRX18039491,SRS15546048,SRA1528514,Emory University,Emory University,2,0.00432,0.90419,0.00171,0.10489,0.9947,0.84737,0.31827,0.50898,28,91,T,B,sc-like readlen,illumina,nextseq,unknown,cdna_unspecified,unknown,sc,single_cell_droplet,10x,,United States,2022-10-26,Juvenile,Juvenile,Heart,Cardiovascular System 76452,SRR24950188,SRX20708357,SRS18001302,SRP444408,PRJNA984676,RNA Seq analyses of myocardium specific hey2 overexpressing zebrafish hearts and Adv Hey2 infected NRVMs,PRJNA984676,Other,To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.,,,,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments rep1,OE1w1,,strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:OE1w replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments rep1,OE1w replicate 1,OE1w replicate 1,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments replicate 1,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,BGISEQ,BGISEQ-500,,SRP444408,,,OE1w1_1.fq.gz OE1w1_2.fq.gz,fastq fastq,6792141900.0,22640473.0,OE1w1 1.fq.gz,0:150 1:150,A:1821318465;C:1573309073;G:1556399365;T:1841095017;N:19980,150,150,,,1821318465,1573309073,1556399365,1841095017,19980,SRX20708357,SRS18001302,SRA1657339,East China Normal University|School of Life Sciences,East China Normal University,2,0.92576,0.91367,0.07095,0.07022,0.74884,0.75012,0.52564,0.52623,150,150,B,B,biological fallback assumption,bgi,bgi,unknown,random_priming,unknown,bulk,unknown,unknown,,China,2023-06-16,Juvenile,Juvenile,Heart,Cardiovascular System 76453,SRR24950189,SRX20708356,SRS18001301,SRP444408,PRJNA984676,RNA Seq analyses of myocardium specific hey2 overexpressing zebrafish hearts and Adv Hey2 infected NRVMs,PRJNA984676,Other,To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.,,,,RNA Seq for control littermate hearts at 1 week post 4 HT treatments rep3,CTL1w3,,strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:CTL1w replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq for control littermate hearts at 1 week post 4 HT treatments rep3,CTL1w replicate 3,CTL1w replicate 3,RNA Seq for control littermate hearts at 1 week post 4 HT treatments replicate 3,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,BGISEQ,BGISEQ-500,,SRP444408,,,CTL1w3_2.fq.gz CTL1w3_1.fq.gz,fastq fastq,6615956700.0,22053189.0,CTL1w3 1.fq.gz,0:150 1:150,A:1790825663;C:1516487260;G:1497904437;T:1810720169;N:19171,150,150,,,1790825663,1516487260,1497904437,1810720169,19171,SRX20708356,SRS18001301,SRA1657339,East China Normal University|School of Life Sciences,East China Normal University,2,0.93183,0.91562,0.07118,0.07012,0.7655,0.7682,0.53912,0.52899,150,150,B,B,biological fallback assumption,bgi,bgi,unknown,random_priming,unknown,bulk,unknown,unknown,,China,2023-06-16,Juvenile,Juvenile,Heart,Cardiovascular System 76454,SRR24950190,SRX20708355,SRS18001300,SRP444408,PRJNA984676,RNA Seq analyses of myocardium specific hey2 overexpressing zebrafish hearts and Adv Hey2 infected NRVMs,PRJNA984676,Other,To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.,,,,RNA Seq for control littermate hearts at 1 week post 4 HT treatments rep2,CTL1w2,,strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:CTL1w replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq for control littermate hearts at 1 week post 4 HT treatments rep2,CTL1w replicate 2,CTL1w replicate 2,RNA Seq for control littermate hearts at 1 week post 4 HT treatments replicate 2,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,BGISEQ,BGISEQ-500,,SRP444408,,,CTL1w2_1.fq.gz CTL1w2_2.fq.gz,fastq fastq,6582133800.0,21940446.0,CTL1w2 1.fq.gz,0:150 1:150,A:1766322301;C:1524014072;G:1506429130;T:1785350030;N:18267,150,150,,,1766322301,1524014072,1506429130,1785350030,18267,SRX20708355,SRS18001300,SRA1657339,East China Normal University|School of Life Sciences,East China Normal University,2,0.93867,0.92389,0.06545,0.06458,0.76357,0.76583,0.53821,0.54255,150,150,B,B,biological fallback assumption,bgi,bgi,unknown,random_priming,unknown,bulk,unknown,unknown,,China,2023-06-16,Juvenile,Juvenile,Heart,Cardiovascular System 76455,SRR24950191,SRX20708354,SRS18001299,SRP444408,PRJNA984676,RNA Seq analyses of myocardium specific hey2 overexpressing zebrafish hearts and Adv Hey2 infected NRVMs,PRJNA984676,Other,To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.,,,,RNA Seq for control littermate hearts at 1 week post 4 HT treatments rep1,CTL1w1,,strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:CTL1w replicate 1|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq for control littermate hearts at 1 week post 4 HT treatments rep1,CTL1w replicate 1,CTL1w replicate 1,RNA Seq for control littermate hearts at 1 week post 4 HT treatments replicate 1,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,BGISEQ,BGISEQ-500,,SRP444408,,,CTL1w1_2.fq.gz CTL1w1_1.fq.gz,fastq fastq,6781172100.0,22603907.0,CTL1w1 1.fq.gz,0:150 1:150,A:1804085074;C:1581571692;G:1567430366;T:1828066356;N:18612,150,150,,,1804085074,1581571692,1567430366,1828066356,18612,SRX20708354,SRS18001299,SRA1657339,East China Normal University|School of Life Sciences,East China Normal University,2,0.93867,0.91921,0.06237,0.06146,0.75858,0.76246,0.53972,0.53069,150,150,B,B,biological fallback assumption,bgi,bgi,unknown,random_priming,unknown,bulk,unknown,unknown,,China,2023-06-16,Juvenile,Juvenile,Heart,Cardiovascular System 76460,SRR24950198,SRX20708347,SRS18001292,SRP444408,PRJNA984676,RNA Seq analyses of myocardium specific hey2 overexpressing zebrafish hearts and Adv Hey2 infected NRVMs,PRJNA984676,Other,To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.,,,,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments rep3,OE1w3,,strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:OE1w replicate 3|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments rep3,OE1w replicate 3,OE1w replicate 3,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments replicate 3,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,BGISEQ,BGISEQ-500,,SRP444408,,,OE1w3_2.fq.gz OE1w3_1.fq.gz,fastq fastq,6593130600.0,21977102.0,OE1w3 1.fq.gz,0:150 1:150,A:1770061078;C:1529682678;G:1510949508;T:1782338558;N:98778,150,150,,,1770061078,1529682678,1510949508,1782338558,98778,SRX20708347,SRS18001292,SRA1657339,East China Normal University|School of Life Sciences,East China Normal University,2,0.93503,0.922,0.06588,0.06574,0.7503,0.75201,0.52969,0.53743,150,150,B,B,biological fallback assumption,bgi,bgi,unknown,random_priming,unknown,bulk,unknown,unknown,,China,2023-06-16,Juvenile,Juvenile,Heart,Cardiovascular System 76461,SRR24950199,SRX20708346,SRS18001291,SRP444408,PRJNA984676,RNA Seq analyses of myocardium specific hey2 overexpressing zebrafish hearts and Adv Hey2 infected NRVMs,PRJNA984676,Other,To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.,,,,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments rep2,OE1w2,,strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:OE1w replicate 2|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments rep2,OE1w replicate 2,OE1w replicate 2,RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments replicate 2,,,RNA-Seq,TRANSCRIPTOMIC,PCR,PAIRED,BGISEQ,BGISEQ-500,,SRP444408,,,OE1w2_2.fq.gz OE1w2_1.fq.gz,fastq fastq,6742724700.0,22475749.0,OE1w2 1.fq.gz,0:150 1:150,A:1802906309;C:1568304698;G:1553625077;T:1817795677;N:92939,150,150,,,1802906309,1568304698,1553625077,1817795677,92939,SRX20708346,SRS18001291,SRA1657339,East China Normal University|School of Life Sciences,East China Normal University,2,0.93354,0.91844,0.06989,0.0686,0.74665,0.74888,0.52723,0.52812,150,150,B,B,biological fallback assumption,bgi,bgi,unknown,random_priming,unknown,bulk,unknown,unknown,,China,2023-06-16,Juvenile,Juvenile,Heart,Cardiovascular System