rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
62,DRR032762,DRX029568,DRS049967,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief taking advantages of Illumina sequencing RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime5 6 3,SAMD00028159,,sample name:Dr prime5 6 3|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime5 6|genotype:wild type|phenotype:wild type|sex:male female and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028159,DRX029568,Dr prime5 6 3,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,1000Application ReadForward1,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028159,,,,3903332800.0,39033328.0,DRR032762,0:100 1:0,A:1050045822;C:908538410;G:900588661;T:1044116537;N:43370,100,0,,,1050045822,908538410,900588661,1044116537,43370,DRX029568,DRS049967,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92761,,0.07976,,0.69126,,0.46568,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
63,DRR032761,DRX029567,DRS049966,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief taking advantages of Illumina sequencing RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime5 6 2,SAMD00028158,,sample name:Dr prime5 6 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime5 6|genotype:wild type|phenotype:wild type|sex:male female and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028158,DRX029567,Dr prime5 6 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,1000Application ReadForward1,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028158,,,,3678549700.0,36785497.0,DRR032761,0:100 1:0,A:986526644;C:857762765;G:853417738;T:980801764;N:40789,100,0,,,986526644,857762765,853417738,980801764,40789,DRX029567,DRS049966,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92689,,0.07872,,0.6928,,0.46577,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
64,DRR032760,DRX029566,DRS049965,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief taking advantages of Illumina sequencing RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime5 6 1,SAMD00028157,,sample name:Dr prime5 6 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime5 6|genotype:wild type|phenotype:wild type|sex:male female and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028157,DRX029566,Dr prime5 6 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,1000Application ReadForward1,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028157,,,,3863129500.0,38631295.0,DRR032760,0:100 1:0,A:1035240477;C:901625010;G:895370149;T:1030851937;N:41927,100,0,,,1035240477,901625010,895370149,1030851937,41927,DRX029566,DRS049965,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92337,,0.07522,,0.69315,,0.46516,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
65,DRR032759,DRX029565,DRS049964,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief taking advantages of Illumina sequencing RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime25 2,SAMD00028156,,sample name:Dr prime25 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime25|genotype:wild type|phenotype:wild type|sex:male female and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028156,DRX029565,Dr prime25 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,1000Application ReadForward1,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028156,,,,3750136100.0,37501361.0,DRR032759,0:100 1:0,A:1013528040;C:866734984;G:862431819;T:1007403208;N:38049,100,0,,,1013528040,866734984,862431819,1007403208,38049,DRX029565,DRS049964,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92019,,0.09079,,0.68304,,0.47083,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
66,DRR032758,DRX029564,DRS049963,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief taking advantages of Illumina sequencing RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 50 individuals,Dr prime25 1,SAMD00028155,,sample name:Dr prime25 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:prime25|genotype:wild type|phenotype:wild type|sex:male female and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028155,DRX029564,Dr prime25 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,1000Application ReadForward1,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028155,,,,3544862700.0,35448627.0,DRR032758,0:100 1:0,A:952135895;C:825841753;G:821757889;T:945087927;N:39236,100,0,,,952135895,825841753,821757889,945087927,39236,DRX029564,DRS049963,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92229,,0.08344,,0.68525,,0.466,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
67,DRR032757,DRX029563,DRS049962,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief taking advantages of Illumina sequencing RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 97 individuals,Dr bud 2,SAMD00028154,,sample name:Dr bud 2|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:bud|genotype:wild type|phenotype:wild type|sex:male female and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028154,DRX029563,Dr bud 2,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,1000Application ReadForward1,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028154,,,,4104778200.0,41047782.0,DRR032757,0:100 1:0,A:1116316188;C:944738800;G:936257056;T:1107423486;N:42670,100,0,,,1116316188,944738800,936257056,1107423486,42670,DRX029563,DRS049962,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92945,,0.10493,,0.73407,,0.47824,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
68,DRR032756,DRX029562,DRS049961,DRP003810,PRJDB3785,EXPANDE project,DRP003810,Other,EXPression AloNg Development and Evolution EXPANDE project aims to identify gene expression profiles expanded during embryogenesis and evolution. In brief taking advantages of Illumina sequencing RNAseq profiles of early to late embryos of 8 chordate species were identified with biological replicates two or more biological replicates.,,,mRNA extracted from pooled embryos of 100 individuals,Dr bud 1,SAMD00028153,,sample name:Dr bud 1|strain:Riken WT Wild Type|tissue type:whole embryo|dev stage:bud|genotype:wild type|phenotype:wild type|sex:male female and mixed,,,,,,,,,Illumina HiSeq 2000 sequencing of SAMD00028153,DRX029562,Dr bud 1,1,Total RNA QIAGEN RNeasy followed by TruSeq,,RNA-Seq,TRANSCRIPTOMIC,other,SINGLE,ILLUMINA,Illumina HiSeq 2000,1000Application ReadForward1,DRP003810,Illumina HiSeq 2000 sequencing of SAMD00028153,,,,4540291000.0,45402910.0,DRR032756,0:100 1:0,A:1237914068;C:1042346110;G:1033172731;T:1226799791;N:58300,100,0,,,1237914068,1042346110,1033172731,1226799791,58300,DRX029562,DRS049961,DRA003460,"UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo","UT-BS|Lab for embryology, Department of Biological Sciences, University of Tokyo",1,0.92628,,0.10478,,0.7391,,0.46461,,100,,B,,usable mapping rate,illumina,hiseq_era,unknown,other,trueseq,bulk,unknown,unknown,,Japan,2017-09-20,Undetermined,Embryo,Whole Organism,All anatomical structures
95,DRR050167,DRX045209,DRS025834,DRP003043,PRJDB4416,Transcriptome analysis in ES1 expressing rods of zebrafish,DRP003043,Transcriptome Analysis,Transcriptome analysis using next generation sequencing system for isolated rods from zebrafish of wild type T?bingen long fin TL line. Transgenic zebrafish expressing ES1 and/or EGFP in rods were used.,,,Zebrafish rods expressing ES1 and EGFP,ES1 3,SAMD00044057,,sample name:ES1 EGFP rod 003|strain:Tubingen long fin|cell type:rod,,,,,,,,,Ion Torrent PGM sequencing of SAMD00044057,DRX045209,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent PGM,1050Application ReadForward1,DRP003043,Ion Torrent PGM sequencing of SAMD00044057,,,,371748120.0,3469843.0,DRR050167,0:107.14,A:107190409;C:79475572;G:83520360;T:101561779;N:0,107,,,,107190409,79475572,83520360,101561779,0,DRX045209,DRS025834,DRA004229,"OSAKA_FB|Kawamura lab., Frontier Biology, Osaka univ.",Osaka university,1,0.81026,,0.26715,,0.86953,,0.52321,,51,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2016-03-09,Undetermined,Undetermined,Fin,Surface Structure
96,DRR050166,DRX045208,DRS025833,DRP003043,PRJDB4416,Transcriptome analysis in ES1 expressing rods of zebrafish,DRP003043,Transcriptome Analysis,Transcriptome analysis using next generation sequencing system for isolated rods from zebrafish of wild type T?bingen long fin TL line. Transgenic zebrafish expressing ES1 and/or EGFP in rods were used.,,,Zebrafish rods expressing ES1 and EGFP,ES1 2,SAMD00044056,,sample name:ES1 EGFP rod 002|strain:Tubingen long fin|cell type:rod,,,,,,,,,Ion Torrent PGM sequencing of SAMD00044056,DRX045208,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent PGM,1280Application ReadForward1,DRP003043,Ion Torrent PGM sequencing of SAMD00044056,,,,425549011.0,3275261.0,DRR050166,0:129.93,A:126640704;C:86415371;G:90822163;T:121670773;N:0,129,,,,126640704,86415371,90822163,121670773,0,DRX045208,DRS025833,DRA004229,"OSAKA_FB|Kawamura lab., Frontier Biology, Osaka univ.",Osaka university,1,0.80223,,0.31429,,0.85861,,0.5272,,57,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2016-03-09,Undetermined,Undetermined,Fin,Surface Structure
97,DRR050165,DRX045207,DRS025832,DRP003043,PRJDB4416,Transcriptome analysis in ES1 expressing rods of zebrafish,DRP003043,Transcriptome Analysis,Transcriptome analysis using next generation sequencing system for isolated rods from zebrafish of wild type T?bingen long fin TL line. Transgenic zebrafish expressing ES1 and/or EGFP in rods were used.,,,Zebrafish rods expressing ES1 and EGFP,ES1 1,SAMD00044055,,sample name:ES1 EGFP rod 001|strain:Tubingen long fin|cell type:rod,,,,,,,,,Ion Torrent PGM sequencing of SAMD00044055,DRX045207,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent PGM,1470Application ReadForward1,DRP003043,Ion Torrent PGM sequencing of SAMD00044055,,,,584599765.0,3941083.0,DRR050165,0:148.33,A:164329030;C:129885620;G:136727379;T:153657736;N:0,148,,,,164329030,129885620,136727379,153657736,0,DRX045207,DRS025832,DRA004229,"OSAKA_FB|Kawamura lab., Frontier Biology, Osaka univ.",Osaka university,1,0.83769,,0.25395,,0.83116,,0.52784,,185,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2016-03-09,Undetermined,Undetermined,Fin,Surface Structure
98,DRR050164,DRX045206,DRS025831,DRP003043,PRJDB4416,Transcriptome analysis in ES1 expressing rods of zebrafish,DRP003043,Transcriptome Analysis,Transcriptome analysis using next generation sequencing system for isolated rods from zebrafish of wild type T?bingen long fin TL line. Transgenic zebrafish expressing ES1 and/or EGFP in rods were used.,,,Zebrafish rods expressing EGFP,EGFP 3,SAMD00044054,,sample name:EGFP rod 003|strain:Tubingen long fin|cell type:rod,,,,,,,,,Ion Torrent PGM sequencing of SAMD00044054,DRX045206,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent PGM,880Application ReadForward1,DRP003043,Ion Torrent PGM sequencing of SAMD00044054,,,,343014114.0,3827762.0,DRR050164,0:89.61,A:99421434;C:73070267;G:77738821;T:92783592;N:0,89,,,,99421434,73070267,77738821,92783592,0,DRX045206,DRS025831,DRA004229,"OSAKA_FB|Kawamura lab., Frontier Biology, Osaka univ.",Osaka university,1,0.78953,,0.25529,,0.87367,,0.52148,,24,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2016-03-09,Undetermined,Undetermined,Fin,Surface Structure
99,DRR050163,DRX045205,DRS025830,DRP003043,PRJDB4416,Transcriptome analysis in ES1 expressing rods of zebrafish,DRP003043,Transcriptome Analysis,Transcriptome analysis using next generation sequencing system for isolated rods from zebrafish of wild type T?bingen long fin TL line. Transgenic zebrafish expressing ES1 and/or EGFP in rods were used.,,,Zebrafish rods expressing EGFP,EGFP 2,SAMD00044053,,sample name:EGFP rod 002|strain:Tubingen long fin|cell type:rod,,,,,,,,,Ion Torrent PGM sequencing of SAMD00044053,DRX045205,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent PGM,1370Application ReadForward1,DRP003043,Ion Torrent PGM sequencing of SAMD00044053,,,,533069427.0,3824140.0,DRR050163,0:139.40,A:152608173;C:113928273;G:120051739;T:146481242;N:0,139,,,,152608173,113928273,120051739,146481242,0,DRX045205,DRS025830,DRA004229,"OSAKA_FB|Kawamura lab., Frontier Biology, Osaka univ.",Osaka university,1,0.80412,,0.26768,,0.85338,,0.52255,,245,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2016-03-09,Undetermined,Undetermined,Fin,Surface Structure
100,DRR050162,DRX045204,DRS025829,DRP003043,PRJDB4416,Transcriptome analysis in ES1 expressing rods of zebrafish,DRP003043,Transcriptome Analysis,Transcriptome analysis using next generation sequencing system for isolated rods from zebrafish of wild type T?bingen long fin TL line. Transgenic zebrafish expressing ES1 and/or EGFP in rods were used.,,,Zebrafish rods expressing EGFP,EGFP 1,SAMD00044052,,sample name:EGFP rod 001|strain:Tubingen long fin|cell type:rod,,,,,,,,,Ion Torrent PGM sequencing of SAMD00044052,DRX045204,1,1,1,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent PGM,1130Application ReadForward1,DRP003043,Ion Torrent PGM sequencing of SAMD00044052,,,,478262965.0,4224064.0,DRR050162,0:113.22,A:136348264;C:104872341;G:113114502;T:123927858;N:0,113,,,,136348264,104872341,113114502,123927858,0,DRX045204,DRS025829,DRA004229,"OSAKA_FB|Kawamura lab., Frontier Biology, Osaka univ.",Osaka university,1,0.86947,,0.29839,,0.83317,,0.51453,,80,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2016-03-09,Undetermined,Undetermined,Fin,Surface Structure
9918,ERR5059480,ERX4865549,ERS5523939,ERP122761,PRJEB39265,RNA dynamics during zebrafish development,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-06-07-2020-15:41:43:771-1183,Other,RNA dynamics during early zebrafish development,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,,aAM 6h rep1,JD AD30 PRPN1970901,,ENA FIRST PUBLIC:2022 07 05T12:06:33Z|organism:Danio rerio|ENA LAST UPDATE:2022 07 05T12:06:33Z|scientific name:Danio rerio|common name:zebrafish|ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,,,,,,,,PromethION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 09 01 2021 19:50:56:183 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,OXFORD_NANOPORE,PromethION,,ERP122761,PromethION sequencing,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,JD-AD30_PRPN197090.tar.gz,nanopore,3739882337.0,3148027.0,ena RUN CENTER FOR GENOMIC REGULATION CRG 09 01 2021 19:50:56:183 1,0:1188.01,A:1054501690;C:834193435;G:847423060;T:1003764152;N:0,1188,,,,1054501690,834193435,847423060,1003764152,0,ERX4865549,ERS5523939,ERA3206712,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),,,,,,,,,,,,B,,usable mapping rate,ont,ont,unknown,poly_a,unknown,bulk,unknown,unknown,,Spain,2022-07-05,Undetermined,Undetermined,Undetermined,Undetermined
9920,ERR4330695,ERX4277529,ERS4811113,ERP122761,PRJEB39265,RNA dynamics during zebrafish development,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-06-07-2020-15:41:43:771-1183,Other,RNA dynamics during early zebrafish development,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,WT 2h rep1,WT 2h rep1,SAMEA7050483,CENTER FOR GENOMIC REGULATION (CRG),ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05|External Id:SAMEA7050483|INSDC center alias:CENTER FOR GENOMIC REGULATION CRG|INSDC center name:CENTER FOR GENOMIC REGULATION CRG|INSDC first public:2022 07 05T12:06:22Z|INSDC last update:2022 07 05T12:06:22Z|INSDC status:public|Submitter Id:JD B2 PDBN005727|common name:zebrafish|sample name:JD B2 PDBN005727,,,,,,,,,PromethION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 09 07 2020 13:48:01:100 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,OXFORD_NANOPORE,PromethION,,ERP122761,PromethION sequencing,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,JD-B2_PDBN005727.tar.gz,fastq,,,ena RUN CENTER FOR GENOMIC REGULATION CRG 09 07 2020 13:48:01:100 1,,,,,,,,,,,,ERX4277529,,ERA2767154,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),,,,,,,,,,,,,,,ont,ont,unknown,poly_a,unknown,bulk,unknown,unknown,,Spain,2022-07-05,Undetermined,Undetermined,Undetermined,Undetermined
9921,ERR4327134,ERX4273968,ERS4808634,ERP122761,PRJEB39265,RNA dynamics during zebrafish development,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-06-07-2020-15:41:43:771-1183,Other,RNA dynamics during early zebrafish development,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,WT 4h rep2,WT 4h rep2,SAMEA7048000,CENTER FOR GENOMIC REGULATION (CRG),ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05|External Id:SAMEA7048000|INSDC center alias:CENTER FOR GENOMIC REGULATION CRG|INSDC center name:CENTER FOR GENOMIC REGULATION CRG|INSDC first public:2022 07 05T12:06:22Z|INSDC last update:2022 07 05T12:06:22Z|INSDC status:public|Submitter Id:JD AM39 PDBN042841|common name:zebrafish|sample name:JD AM39 PDBN042841,,,,,,,,,PromethION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 07 07 2020 16:36:02:084 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,OXFORD_NANOPORE,PromethION,,ERP122761,PromethION sequencing,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,JD-AM39_PDBN042841.tar.gz,nanopore,719646261.0,897768.0,ena RUN CENTER FOR GENOMIC REGULATION CRG 07 07 2020 16:36:02:084 1,0:801.59,A:210217908;C:152963718;G:157393834;T:199070801;N:0,801,,,,210217908,152963718,157393834,199070801,0,ERX4273968,ERS4808634,ERA2764800,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),,,,,,,,,,,,T,,under 1.2% mapping rate,ont,ont,unknown,poly_a,unknown,bulk,unknown,unknown,,Spain,2022-07-05,Undetermined,Undetermined,Undetermined,Undetermined
9922,ERR4330696,ERX4277530,ERS4811114,ERP122761,PRJEB39265,RNA dynamics during zebrafish development,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-06-07-2020-15:41:43:771-1183,Other,RNA dynamics during early zebrafish development,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,WT 4h rep1,WT 4h rep1,JD C3 PDBN006177,,ENA FIRST PUBLIC:2022 07 05T12:06:22Z|organism:Danio rerio|ENA LAST UPDATE:2022 07 05T12:06:22Z|scientific name:Danio rerio|common name:zebrafish|ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,,,,,,,,PromethION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 09 07 2020 13:48:01:100 2,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,OXFORD_NANOPORE,PromethION,,ERP122761,PromethION sequencing,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,JD-C3_PDBN006177.tar.gz,nanopore,4240799932.0,4331689.0,ena RUN CENTER FOR GENOMIC REGULATION CRG 09 07 2020 13:48:01:100 2,0:979.02,A:1229803846;C:914476674;G:943703560;T:1152815852;N:0,979,,,,1229803846,914476674,943703560,1152815852,0,ERX4277530,ERS4811114,ERA2767154,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),,,,,,,,,,,,B,,usable mapping rate,ont,ont,unknown,poly_a,unknown,bulk,unknown,unknown,,Spain,2022-07-05,Undetermined,Undetermined,Undetermined,Undetermined
9923,ERR4327135,ERX4273969,ERS4808635,ERP122761,PRJEB39265,RNA dynamics during zebrafish development,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-06-07-2020-15:41:43:771-1183,Other,RNA dynamics during early zebrafish development,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,WT 6h rep1,WT 6h rep1,JD AC29 PDBN024889,,ENA FIRST PUBLIC:2022 07 05T12:06:22Z|organism:Danio rerio|ENA LAST UPDATE:2022 07 05T12:06:22Z|scientific name:Danio rerio|common name:zebrafish|ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,,,,,,,,PromethION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 07 07 2020 16:36:02:084 2,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,OXFORD_NANOPORE,PromethION,,ERP122761,PromethION sequencing,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,JD-AC29_PDBN024889.tar.gz,nanopore,1900324756.0,2013035.0,ena RUN CENTER FOR GENOMIC REGULATION CRG 07 07 2020 16:36:02:084 2,0:944.01,A:549431032;C:411510218;G:422103800;T:517279706;N:0,944,,,,549431032,411510218,422103800,517279706,0,ERX4273969,ERS4808635,ERA2764800,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),,,,,,,,,,,,T,,long read,ont,ont,unknown,poly_a,unknown,bulk,unknown,unknown,,Spain,2022-07-05,Undetermined,Undetermined,Undetermined,Undetermined
9924,ERR4326350,ERX4273208,ERS4808398,ERP122761,PRJEB39265,RNA dynamics during zebrafish development,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-06-07-2020-15:41:43:771-1183,Other,RNA dynamics during early zebrafish development,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,430 LNA 6h rep1,430 LNA 6h rep1,SAMEA7047764,CENTER FOR GENOMIC REGULATION (CRG),ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05|External Id:SAMEA7047764|INSDC center alias:CENTER FOR GENOMIC REGULATION CRG|INSDC center name:CENTER FOR GENOMIC REGULATION CRG|INSDC first public:2022 07 05T12:06:22Z|INSDC last update:2022 07 05T12:06:22Z|INSDC status:public|Submitter Id:JD H8 PDBN059569|common name:zebrafish|sample name:JD H8 PDBN059569,,,,,,,,,PromethION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 07 07 2020 10:25:22:388 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,OXFORD_NANOPORE,PromethION,,ERP122761,PromethION sequencing,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,JD-H8_PDBN059569.tar.gz,nanopore,722817654.0,657296.0,ena RUN CENTER FOR GENOMIC REGULATION CRG 07 07 2020 10:25:22:388 1,0:1099.68,A:206996491;C:157022109;G:155085437;T:203713617;N:0,1099,,,,206996491,157022109,155085437,203713617,0,ERX4273208,ERS4808398,ERA2764399,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),,,,,,,,,,,,T,,under 1.2% mapping rate,ont,ont,unknown,poly_a,unknown,bulk,unknown,unknown,,Spain,2022-07-05,Undetermined,Undetermined,Undetermined,Undetermined
9925,ERR4335436,ERX4282181,ERS4818366,ERP122761,PRJEB39265,RNA dynamics during zebrafish development,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-06-07-2020-15:41:43:771-1183,Other,RNA dynamics during early zebrafish development,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,WT 6h rep2,WT 6h rep2,JD W23 PRPN039928,,ENA FIRST PUBLIC:2022 07 05T12:06:24Z|organism:Danio rerio|ENA LAST UPDATE:2022 07 05T12:06:24Z|scientific name:Danio rerio|common name:zebrafish|ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,,,,,,,,PromethION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 13 07 2020 18:19:23:456 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,OXFORD_NANOPORE,PromethION,,ERP122761,PromethION sequencing,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,JD-W23_PRPN039928.tar.gz,nanopore,1268761319.0,1385621.0,ena RUN CENTER FOR GENOMIC REGULATION CRG 13 07 2020 18:19:23:457 1,0:915.66,A:366823862;C:275507684;G:284634548;T:341795225;N:0,915,,,,366823862,275507684,284634548,341795225,0,ERX4282181,ERS4818366,ERA2769006,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),,,,,,,,,,,,T,,under 1.2% mapping rate,ont,ont,unknown,poly_a,unknown,bulk,unknown,unknown,,Spain,2022-07-05,Undetermined,Undetermined,Undetermined,Undetermined
9926,ERR4321680,ERX4268538,ERS4808125,ERP122761,PRJEB39265,RNA dynamics during zebrafish development,ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-06-07-2020-15:41:43:771-1183,Other,RNA dynamics during early zebrafish development,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,WT 0h rep1,WT 0h rep1,JD A1 GDDN003032,,ENA FIRST PUBLIC:2022 07 05T12:06:22Z|organism:Danio rerio|ENA LAST UPDATE:2022 07 05T12:06:22Z|scientific name:Danio rerio|common name:zebrafish|ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,,,,,,,,,GridION sequencing,ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 06 07 2020 17:45:26:236 1,unspecified,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,SINGLE,OXFORD_NANOPORE,GridION,,ERP122761,GridION sequencing,ENA FIRST PUBLIC:2022 07 05|ENA LAST UPDATE:2022 07 05,JD-A1_GDDN003032.tar.gz,nanopore,753417826.0,698774.0,ena RUN CENTER FOR GENOMIC REGULATION CRG 06 07 2020 17:45:26:236 1,0:1078.20,A:214525685;C:165042952;G:171160615;T:202688574;N:0,1078,,,,214525685,165042952,171160615,202688574,0,ERX4268538,ERS4808125,ERA2763718,CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive,CENTER FOR GENOMIC REGULATION (CRG),,,,,,,,,,,,T,,long read,ont,ont,unknown,poly_a,unknown,bulk,unknown,unknown,,Spain,2022-07-05,Undetermined,Undetermined,Undetermined,Undetermined
11833,ERR11872224,ERX11269666,ERS16284435,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 9,SAMEA114295326,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295326|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 9|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 9|scientific name:Danio rerio|strain:AB|tank:T13,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 9 s,Sample 9 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1100sR1436.fastq.gz,fastq,1961832438.0,38853673.0,E MTAB 13269:Sample 9,0:50.49,A:498124499;C:481739620;G:459005215;T:520487633;N:2475471,50,,,,498124499,481739620,459005215,520487633,2475471,ERX11269666,ERS16284435,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11834,ERR11872220,ERX11269662,ERS16284431,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 5,SAMEA114295322,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:27Z|ENA LAST UPDATE:2024 01 01T01:03:27Z|External Id:SAMEA114295322|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:27Z|INSDC last update:2024 01 01T01:03:27Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 5|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 5|scientific name:Danio rerio|strain:AB|tank:T8,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 5 s,Sample 5 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1137sR1431.fastq.gz,fastq,1384318515.0,27436121.0,E MTAB 13269:Sample 5,0:50.46,A:351376207;C:340294254;G:322773331;T:366105473;N:3769250,50,,,,351376207,340294254,322773331,366105473,3769250,ERX11269662,ERS16284431,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11835,ERR11872216,ERX11269658,ERS16284427,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 1,SAMEA114295318,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:27Z|ENA LAST UPDATE:2024 01 01T01:03:27Z|External Id:SAMEA114295318|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:27Z|INSDC last update:2024 01 01T01:03:27Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 1|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 1|scientific name:Danio rerio|strain:AB|tank:T7,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 1 s,Sample 1 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1067sR1425.fastq.gz,fastq,2542543911.0,50536210.0,E MTAB 13269:Sample 1,0:50.31,A:650946248;C:611164311;G:577961102;T:679442750;N:23029500,50,,,,650946248,611164311,577961102,679442750,23029500,ERX11269658,ERS16284427,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11836,ERR11872218,ERX11269660,ERS16284429,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 3,SAMEA114295320,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:27Z|ENA LAST UPDATE:2024 01 01T01:03:27Z|External Id:SAMEA114295320|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:27Z|INSDC last update:2024 01 01T01:03:27Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 3|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 3|scientific name:Danio rerio|strain:AB|tank:T7,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 3 s,Sample 3 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1100sR1428.fastq.gz,fastq,1504139674.0,29976579.0,E MTAB 13269:Sample 3,0:50.18,A:378815411;C:361930389;G:348289715;T:391168927;N:23935232,50,,,,378815411,361930389,348289715,391168927,23935232,ERX11269660,ERS16284429,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11837,ERR11872219,ERX11269661,ERS16284430,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 4,SAMEA114295321,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:27Z|ENA LAST UPDATE:2024 01 01T01:03:27Z|External Id:SAMEA114295321|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:27Z|INSDC last update:2024 01 01T01:03:27Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 4|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 4|scientific name:Danio rerio|strain:AB|tank:T8,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 4 s,Sample 4 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1067sR1429.fastq.gz,fastq,1287164622.0,25539878.0,E MTAB 13269:Sample 4,0:50.40,A:326779989;C:313960441;G:299210114;T:340187543;N:7026535,50,,,,326779989,313960441,299210114,340187543,7026535,ERX11269661,ERS16284430,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11838,ERR11872222,ERX11269664,ERS16284433,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 7,SAMEA114295324,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:27Z|ENA LAST UPDATE:2024 01 01T01:03:27Z|External Id:SAMEA114295324|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:27Z|INSDC last update:2024 01 01T01:03:27Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 7|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 7|scientific name:Danio rerio|strain:AB|tank:T13,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 7 s,Sample 7 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1067sR1433.fastq.gz,fastq,2055723538.0,40748974.0,E MTAB 13269:Sample 7,0:50.45,A:530988871;C:494680653;G:471905883;T:551730327;N:6417804,50,,,,530988871,494680653,471905883,551730327,6417804,ERX11269664,ERS16284433,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11839,ERR11872221,ERX11269663,ERS16284432,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 6,SAMEA114295323,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:27Z|ENA LAST UPDATE:2024 01 01T01:03:27Z|External Id:SAMEA114295323|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:27Z|INSDC last update:2024 01 01T01:03:27Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 6|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 6|scientific name:Danio rerio|strain:AB|tank:T8,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 6 s,Sample 6 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1100sR1432.fastq.gz,fastq,1541862753.0,30534298.0,E MTAB 13269:Sample 6,0:50.50,A:390914024;C:378834682;G:362325696;T:407660220;N:2128131,50,,,,390914024,378834682,362325696,407660220,2128131,ERX11269663,ERS16284432,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11840,ERR11872217,ERX11269659,ERS16284428,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 2,SAMEA114295319,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:27Z|ENA LAST UPDATE:2024 01 01T01:03:27Z|External Id:SAMEA114295319|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:27Z|INSDC last update:2024 01 01T01:03:27Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 2|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 2|scientific name:Danio rerio|strain:AB|tank:T7,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 2 s,Sample 2 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1067sR1427.fastq.gz,fastq,1120463267.0,22442762.0,E MTAB 13269:Sample 2,0:49.93,A:283544047;C:261601041;G:250663913;T:294648349;N:30005917,49,,,,283544047,261601041,250663913,294648349,30005917,ERX11269659,ERS16284428,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11841,ERR11872223,ERX11269665,ERS16284434,ERP150458,PRJEB65329,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-13269,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 8,SAMEA114295325,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295325|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13269:Sample 8|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13269:Sample 8|scientific name:Danio rerio|strain:AB|tank:T13,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 13269:Sample 8 s,Sample 8 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. simutaneous RNA and protein extraction was performed from the homogenised samples using Macherey & Nagel RNA/protein extraction kit. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150458,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1067sR1435.fastq.gz,fastq,2492217928.0,49578248.0,E MTAB 13269:Sample 8,0:50.27,A:633127675;C:599191649;G:572355526;T:659345988;N:28197090,50,,,,633127675,599191649,572355526,659345988,28197090,ERX11269665,ERS16284434,ERA26965226,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11842,ERR11872230,ERX11269672,ERS16284439,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 4,SAMEA114295330,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295330|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 4|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 4|scientific name:Danio rerio|strain:AB|tank:T8,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 4 s,Sample 4 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s2-R1718-dre-nc-metalaxyl_Eilebrecht.fastq.gz,fastq,3090739917.0,61141982.0,E MTAB 13270:Sample 4,0:50.55,A:757773770;C:707158595;G:708507725;T:915894260;N:1405567,50,,,,757773770,707158595,708507725,915894260,1405567,ERX11269672,ERS16284439,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11843,ERR11872228,ERX11269670,ERS16284437,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 2,SAMEA114295328,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295328|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 2|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 2|scientific name:Danio rerio|strain:AB|tank:T6,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 2 s,Sample 2 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s1-R1716-dre-C2-metalaxyl_Eilebrecht.fastq.gz,fastq,2700642168.0,53433052.0,E MTAB 13270:Sample 2,0:50.54,A:669595678;C:619368901;G:590427891;T:819827582;N:1422116,50,,,,669595678,619368901,590427891,819827582,1422116,ERX11269670,ERS16284437,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11844,ERR11872227,ERX11269669,ERS16284436,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 1,SAMEA114295327,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295327|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 1|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 1|scientific name:Danio rerio|strain:AB|tank:T6,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 1 s,Sample 1 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s1-R1714-dre-nc-metalaxyl_Eilebrecht.fastq.gz,fastq,2274694218.0,45004376.0,E MTAB 13270:Sample 1,0:50.54,A:562285999;C:518447937;G:499619290;T:693250049;N:1090943,50,,,,562285999,518447937,499619290,693250049,1090943,ERX11269669,ERS16284436,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11845,ERR11872231,ERX11269673,ERS16284440,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 5,SAMEA114295331,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295331|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 5|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 5|scientific name:Danio rerio|strain:AB|tank:T8,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 5 s,Sample 5 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s2-R1720-dre-C2-metalaxyl_Eilebrecht.fastq.gz,fastq,2777053633.0,54933357.0,E MTAB 13270:Sample 5,0:50.55,A:677084481;C:642868295;G:614554344;T:841210183;N:1336330,50,,,,677084481,642868295,614554344,841210183,1336330,ERX11269673,ERS16284440,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11846,ERR11872229,ERX11269671,ERS16284438,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 3,SAMEA114295329,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295329|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 3|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 3|scientific name:Danio rerio|strain:AB|tank:T6,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 3 s,Sample 3 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s1-R1717-dre-C3-metalaxyl_Eilebrecht.fastq.gz,fastq,2503234144.0,49524111.0,E MTAB 13270:Sample 3,0:50.55,A:612103203;C:572993175;G:554869755;T:761847743;N:1420268,50,,,,612103203,572993175,554869755,761847743,1420268,ERX11269671,ERS16284438,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11847,ERR11872233,ERX11269675,ERS16284442,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 7,SAMEA114295333,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295333|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 7|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 7|scientific name:Danio rerio|strain:AB|tank:T9,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 7 s,Sample 7 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s3-R1722-dre-nc-metalaxyl_Eilebrecht.fastq.gz,fastq,2210069991.0,43725261.0,E MTAB 13270:Sample 7,0:50.54,A:543075905;C:511224703;G:487348154;T:667396647;N:1024582,50,,,,543075905,511224703,487348154,667396647,1024582,ERX11269675,ERS16284442,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11848,ERR11872234,ERX11269676,ERS16284443,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 8,SAMEA114295334,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295334|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 8|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 8|scientific name:Danio rerio|strain:AB|tank:T9,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 8 s,Sample 8 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s3-R1724-dre-C2-metalaxyl_Eilebrecht.fastq.gz,fastq,2289618271.0,45294560.0,E MTAB 13270:Sample 8,0:50.55,A:560540697;C:527453035;G:507883622;T:692636061;N:1104856,50,,,,560540697,527453035,507883622,692636061,1104856,ERX11269676,ERS16284443,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11849,ERR11872235,ERX11269677,ERS16284444,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 9,SAMEA114295335,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295335|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 9|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 9|scientific name:Danio rerio|strain:AB|tank:T9,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 9 s,Sample 9 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s3-R1725-dre-C3-metalaxyl_Eilebrecht.fastq.gz,fastq,2164282095.0,42813111.0,E MTAB 13270:Sample 9,0:50.55,A:526798074;C:499625113;G:480175291;T:656576036;N:1107581,50,,,,526798074,499625113,480175291,656576036,1107581,ERX11269677,ERS16284444,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
11850,ERR11872232,ERX11269674,ERS16284441,ERP150460,PRJEB65331,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E-MTAB-13270,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide metalaxyl a nucleic acids metabolism inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to metalaxyl according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,,Protocols: : at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,Sample 6,SAMEA114295332,Fraunhofer Institute for Molecular Biology and Aplied Ecology (IME),ENA FIRST PUBLIC:2024 01 01T01:03:26Z|ENA LAST UPDATE:2024 01 01T01:03:26Z|External Id:SAMEA114295332|INSDC center name:Fraunhofer Institute for Molecular Biology and Aplied Ecology IME|INSDC first public:2024 01 01T01:03:26Z|INSDC last update:2024 01 01T01:03:26Z|INSDC status:public|Submitter Id:E MTAB 13270:Sample 6|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 13270:Sample 6|scientific name:Danio rerio|strain:AB|tank:T8,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,E MTAB 13270:Sample 6 s,Sample 6 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of metalaxyl high exposure and low exposure concentrations in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA.,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP150460,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups,ENA FIRST PUBLIC:2024 01 01|ENA LAST UPDATE:2024 01 01,p1201s2-R1721-dre-C3-metalaxyl_Eilebrecht.fastq.gz,fastq,2333796132.0,46171402.0,E MTAB 13270:Sample 6,0:50.55,A:571724946;C:541769310;G:513423860;T:705671180;N:1206836,50,,,,571724946,541769310,513423860,705671180,1206836,ERX11269674,ERS16284441,ERA26965713,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-01-01,Undetermined,Embryo,Whole Organism,All anatomical structures
19140,ERR13769875,ERX13170676,ERS21156063,ERP165027,PRJEB81172,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-14536,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2025 04 30|ENA LAST UPDATE:2025 04 30,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. total RNA and Protein were extracted from the tissue lysate using using the NucleoSpin® RNA/Protein kit Macherey & Nagel Düren Germany. according to the manufacturer's protocol. Nanodrop 2000 spectrophotometer Thermo Scientific was used to measure and normalize the obtained RNA concentrations. The quality of the extracted RNA samples was checked using RNA 6000 Nano kit in 2100 Bioanalyzer System Agilent Santa Clara USA. Samples were stored at 80°C. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 1,SAMEA116134556,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA first public:2025 04 30|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC status:public|Submitter Id:E MTAB 14536:Sample 1|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 14536:Sample 1|scientific name:Danio rerio|strain:AB|tank:T7,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 14536:Sample 1 s,Sample 1 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. total RNA and Protein were extracted from the tissue lysate using using the NucleoSpin® RNA/Protein kit Macherey & Nagel Düren Germany. according to the manufacturer's protocol. Nanodrop 2000 spectrophotometer Thermo Scientific was used to measure and normalize the obtained RNA concentrations. The quality of the extracted RNA samples was checked using RNA 6000 Nano kit in 2100 Bioanalyzer System Agilent Santa Clara USA. Samples were stored at 80°C. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP165027,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2025 04 30|ENA LAST UPDATE:2025 04 30,p1067sR1426.fastq.gz,fastq,1044742413.0,20762139.0,E MTAB 14536:Sample 1,0:50.32,A:250471042;C:265283643;G:251710894;T:266877610;N:10399224,50,,,,250471042,265283643,251710894,266877610,10399224,ERX13170676,ERS21156063,ERA30873015,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2025-04-30,Undetermined,Embryo,Whole Organism,All anatomical structures
19141,ERR13769876,ERX13170677,ERS21156064,ERP165027,PRJEB81172,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-14536,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2025 04 30|ENA LAST UPDATE:2025 04 30,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. total RNA and Protein were extracted from the tissue lysate using using the NucleoSpin® RNA/Protein kit Macherey & Nagel Düren Germany. according to the manufacturer's protocol. Nanodrop 2000 spectrophotometer Thermo Scientific was used to measure and normalize the obtained RNA concentrations. The quality of the extracted RNA samples was checked using RNA 6000 Nano kit in 2100 Bioanalyzer System Agilent Santa Clara USA. Samples were stored at 80°C. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 2,SAMEA116134557,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA first public:2025 04 30|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC status:public|Submitter Id:E MTAB 14536:Sample 2|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 14536:Sample 2|scientific name:Danio rerio|strain:AB|tank:T8,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 14536:Sample 2 s,Sample 2 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. total RNA and Protein were extracted from the tissue lysate using using the NucleoSpin® RNA/Protein kit Macherey & Nagel Düren Germany. according to the manufacturer's protocol. Nanodrop 2000 spectrophotometer Thermo Scientific was used to measure and normalize the obtained RNA concentrations. The quality of the extracted RNA samples was checked using RNA 6000 Nano kit in 2100 Bioanalyzer System Agilent Santa Clara USA. Samples were stored at 80°C. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP165027,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2025 04 30|ENA LAST UPDATE:2025 04 30,p1067sR1430.fastq.gz,fastq,1360497810.0,27516448.0,E MTAB 14536:Sample 2,0:49.44,A:337559200;C:309495128;G:294605904;T:352033780;N:66803798,49,,,,337559200,309495128,294605904,352033780,66803798,ERX13170677,ERS21156064,ERA30873015,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2025-04-30,Undetermined,Embryo,Whole Organism,All anatomical structures
19142,ERR13769877,ERX13170678,ERS21156065,ERP165027,PRJEB81172,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E-MTAB-14536,Transcriptome Analysis,In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide difenoconazole a sterol biosynthesis inhibitor according to Fungicide Resistance Action Committee FRAC classification. Zebrafish embryos were exposed to difenoconazole according to OECD guidelines OECD test No. 236. At the end of exposure time 96 hours simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina HiSeq 4000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.,ENA FIRST PUBLIC:2025 04 30|ENA LAST UPDATE:2025 04 30,,Protocols: at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. total RNA and Protein were extracted from the tissue lysate using using the NucleoSpin® RNA/Protein kit Macherey & Nagel Düren Germany. according to the manufacturer's protocol. Nanodrop 2000 spectrophotometer Thermo Scientific was used to measure and normalize the obtained RNA concentrations. The quality of the extracted RNA samples was checked using RNA 6000 Nano kit in 2100 Bioanalyzer System Agilent Santa Clara USA. Samples were stored at 80°C. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,Sample 3,SAMEA116134558,Fraunhofer Institute for Molecular Biology and Applied Ecology (IME),ENA first public:2025 04 30|INSDC center name:Fraunhofer Institute for Molecular Biology and Applied Ecology IME|INSDC status:public|Submitter Id:E MTAB 14536:Sample 3|age:96|broker name:ArrayExpress|collection date:not collected|common name:zebrafish|genotype:wild type genotype|geographic location country and/or sea:not collected|isolate:not applicable|organism part:whole organism|sample name:E MTAB 14536:Sample 3|scientific name:Danio rerio|strain:AB|tank:T13,,,,,,,,,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,E MTAB 14536:Sample 3 s,Sample 3 s,mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,at the end of exposure 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep 24 homogeniser MP Biomedicals Irvine USA at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for xxx hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub lethal concentrations of difenoconazole high exposure and low exposure concentrations in addition to untreated control groups. total RNA and Protein were extracted from the tissue lysate using using the NucleoSpin® RNA/Protein kit Macherey & Nagel Düren Germany. according to the manufacturer's protocol. Nanodrop 2000 spectrophotometer Thermo Scientific was used to measure and normalize the obtained RNA concentrations. The quality of the extracted RNA samples was checked using RNA 6000 Nano kit in 2100 Bioanalyzer System Agilent Santa Clara USA. Samples were stored at 80°C. RNA libraries were prepared from RNA extracts with RNA integrity number RIN values > 8 using polyA RNA purification and the TruSeq RNA Library Prep Kit v2 Illumina San Diego USA,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,ERP165027,Illumina HiSeq 4000 sequencing; mRNA Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups,ENA FIRST PUBLIC:2025 04 30|ENA LAST UPDATE:2025 04 30,p1067sR1434.fastq.gz,fastq,1537549016.0,31165038.0,E MTAB 14536:Sample 3,0:49.34,A:379537732;C:349448626;G:332769302;T:392738674;N:83054682,49,,,,379537732,349448626,332769302,392738674,83054682,ERX13170678,ERS21156065,ERA30873015,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2025-04-30,Undetermined,Embryo,Whole Organism,All anatomical structures
25320,SRR25810878,SRX21533024,SRS18745490,SRP457576,PRJNA1010780,Effect of egr3 knockout on gene expression of dissected zebrafish hearts,GSE241935,Transcriptome Analysis,To screen for Egr3 targets mediating cardiac valve development we assessed gene expression on dissected hearts of egr3 mutants and wild type siblings. Overall design: To evaluate the transcriptional differences underlying the egr3 mutant phenotype we conducted a bulk RNA seq analysis in dissected zebrafish hearts at 48 hpf. We dissected 20 hearts for each biological duplicate of egr3 mutant and wild type sibling samples in cold DMEM with 10% FBS. Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12µl of RNase free water. Analysis of the obtained reads confirmed the 11bp deletion in the mutant samples. 1. Boezio G. L. M. et al. The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth. Dis Model Mech 16 2023. https://doi.org:10.1242/dmm.049571,,pubmed:38748804,,egr3 wild type siblings 2,GSM7745909,,source name:Heart|tissue:Heart|genotype:bns577+/+|geo loc name:missing|collection date:missing,egr3 wild type siblings 2,Trimmomatic version 0.39 was employed to trim reads post a quality drop below a mean of Q20 in a window of 20 nucleotides and keeping only filtered reads longer than 15 nucleotides Reads were aligned versus Ensembl zebrafish genome version danRer11 Ensembl release 104 with STAR 2.7.10a Aligned reads were filtered to remove: duplicates with Picard 2.27.1 Picard: A set of tools in Java for working with next generation sequencing data in the BAM format multi mapping ribosomal or mitochondrial reads. Gene counts were established with featureCounts 2.0.2 by aggregating reads overlapping exons excluding those overlapping multiple genes Liao et al. featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. The raw count matrix was normalized with DESeq2 version 1.30.1 Love et al. Moderated estimation of fold change and dispersion for RNA Seq data with DESeq2 and batch corrected using CountClust each biological replicate = one batch Dey K et al. Visualizing the structure of RNA seq expression data using grade of membership models. Genes were classified as significantly differentially expressed at average count > 5 multiple testing adjusted p value < 0.05 and 0.585 < log2FC > 0.585. Assembly: danRer11 Supplementary files format and content: libryr size notmalize amd batch corrected counts,Heart,,Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12μl of RNase free water 10ng total RNA was used for SMART Seq® v4 Ultra® Low Input RNA Kit Takara Bio.,,tissue:Heart|genotype:bns577+/+,GSM7745909,GSM7745909: egr3 wild type siblings 2; Danio rerio; RNA Seq,GSM7745909 r1,GSM7745909,1,Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12μl of RNase free water 10ng total RNA was used for SMART Seq® v4 Ultra® Low Input RNA Kit Takara Bio.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,,SRP457576,,loader:fastq load.py,E22_3626_Lib_Agatha_egr3_WT2_R1.fastq.gz,fastq,3473319384.0,49279465.0,GSM7745909 r1,0:70.48,A:943794847;C:792135324;G:792042625;T:945083925;N:262663,70,,,,943794847,792135324,792042625,945083925,262663,SRX21533024,SRS18745490,SRA1702458,MPI for heart and lung research,MPI for heart and lung research,1,0.68549,,0.04588,,0.76865,,0.44964,,71,,B,,usable mapping rate,illumina,nextseq_v2,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,Germany,2023-08-30,Undetermined,Undetermined,Heart,Cardiovascular System
25321,SRR25810879,SRX21533023,SRS18745489,SRP457576,PRJNA1010780,Effect of egr3 knockout on gene expression of dissected zebrafish hearts,GSE241935,Transcriptome Analysis,To screen for Egr3 targets mediating cardiac valve development we assessed gene expression on dissected hearts of egr3 mutants and wild type siblings. Overall design: To evaluate the transcriptional differences underlying the egr3 mutant phenotype we conducted a bulk RNA seq analysis in dissected zebrafish hearts at 48 hpf. We dissected 20 hearts for each biological duplicate of egr3 mutant and wild type sibling samples in cold DMEM with 10% FBS. Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12µl of RNase free water. Analysis of the obtained reads confirmed the 11bp deletion in the mutant samples. 1. Boezio G. L. M. et al. The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth. Dis Model Mech 16 2023. https://doi.org:10.1242/dmm.049571,,pubmed:38748804,,egr3 wild type siblings 1,GSM7745908,,source name:Heart|tissue:Heart|genotype:bns577+/+|geo loc name:missing|collection date:missing,egr3 wild type siblings 1,Trimmomatic version 0.39 was employed to trim reads post a quality drop below a mean of Q20 in a window of 20 nucleotides and keeping only filtered reads longer than 15 nucleotides Reads were aligned versus Ensembl zebrafish genome version danRer11 Ensembl release 104 with STAR 2.7.10a Aligned reads were filtered to remove: duplicates with Picard 2.27.1 Picard: A set of tools in Java for working with next generation sequencing data in the BAM format multi mapping ribosomal or mitochondrial reads. Gene counts were established with featureCounts 2.0.2 by aggregating reads overlapping exons excluding those overlapping multiple genes Liao et al. featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. The raw count matrix was normalized with DESeq2 version 1.30.1 Love et al. Moderated estimation of fold change and dispersion for RNA Seq data with DESeq2 and batch corrected using CountClust each biological replicate = one batch Dey K et al. Visualizing the structure of RNA seq expression data using grade of membership models. Genes were classified as significantly differentially expressed at average count > 5 multiple testing adjusted p value < 0.05 and 0.585 < log2FC > 0.585. Assembly: danRer11 Supplementary files format and content: libryr size notmalize amd batch corrected counts,Heart,,Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12μl of RNase free water 10ng total RNA was used for SMART Seq® v4 Ultra® Low Input RNA Kit Takara Bio.,,tissue:Heart|genotype:bns577+/+,GSM7745908,GSM7745908: egr3 wild type siblings 1; Danio rerio; RNA Seq,GSM7745908 r1,GSM7745908,1,Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12μl of RNase free water 10ng total RNA was used for SMART Seq® v4 Ultra® Low Input RNA Kit Takara Bio.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,,SRP457576,,loader:fastq load.py,E22_3626_Lib_Agatha_egr3_WT1_R1.fastq.gz,fastq,3909248616.0,55045571.0,GSM7745908 r1,0:71.02,A:1048107387;C:905014546;G:907455489;T:1048528690;N:142504,71,,,,1048107387,905014546,907455489,1048528690,142504,SRX21533023,SRS18745489,SRA1702458,MPI for heart and lung research,MPI for heart and lung research,1,0.94958,,0.06298,,0.74819,,0.45762,,72,,B,,usable mapping rate,illumina,nextseq_v2,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,Germany,2023-08-30,Undetermined,Undetermined,Heart,Cardiovascular System
25322,SRR25810880,SRX21533022,SRS18745488,SRP457576,PRJNA1010780,Effect of egr3 knockout on gene expression of dissected zebrafish hearts,GSE241935,Transcriptome Analysis,To screen for Egr3 targets mediating cardiac valve development we assessed gene expression on dissected hearts of egr3 mutants and wild type siblings. Overall design: To evaluate the transcriptional differences underlying the egr3 mutant phenotype we conducted a bulk RNA seq analysis in dissected zebrafish hearts at 48 hpf. We dissected 20 hearts for each biological duplicate of egr3 mutant and wild type sibling samples in cold DMEM with 10% FBS. Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12µl of RNase free water. Analysis of the obtained reads confirmed the 11bp deletion in the mutant samples. 1. Boezio G. L. M. et al. The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth. Dis Model Mech 16 2023. https://doi.org:10.1242/dmm.049571,,pubmed:38748804,,egr3 mutants 2,GSM7745907,,source name:Heart|tissue:Heart|genotype:bns577 / |geo loc name:missing|collection date:missing,egr3 mutants 2,Trimmomatic version 0.39 was employed to trim reads post a quality drop below a mean of Q20 in a window of 20 nucleotides and keeping only filtered reads longer than 15 nucleotides Reads were aligned versus Ensembl zebrafish genome version danRer11 Ensembl release 104 with STAR 2.7.10a Aligned reads were filtered to remove: duplicates with Picard 2.27.1 Picard: A set of tools in Java for working with next generation sequencing data in the BAM format multi mapping ribosomal or mitochondrial reads. Gene counts were established with featureCounts 2.0.2 by aggregating reads overlapping exons excluding those overlapping multiple genes Liao et al. featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. The raw count matrix was normalized with DESeq2 version 1.30.1 Love et al. Moderated estimation of fold change and dispersion for RNA Seq data with DESeq2 and batch corrected using CountClust each biological replicate = one batch Dey K et al. Visualizing the structure of RNA seq expression data using grade of membership models. Genes were classified as significantly differentially expressed at average count > 5 multiple testing adjusted p value < 0.05 and 0.585 < log2FC > 0.585. Assembly: danRer11 Supplementary files format and content: libryr size notmalize amd batch corrected counts,Heart,,Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12μl of RNase free water 10ng total RNA was used for SMART Seq® v4 Ultra® Low Input RNA Kit Takara Bio.,,tissue:Heart|genotype:bns577 / ,GSM7745907,GSM7745907: egr3 mutants 2; Danio rerio; RNA Seq,GSM7745907 r1,GSM7745907,1,Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12μl of RNase free water 10ng total RNA was used for SMART Seq® v4 Ultra® Low Input RNA Kit Takara Bio.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,,SRP457576,,loader:fastq load.py,E22_3626_Lib_Agatha_egr3_Mut2_R1.fastq.gz,fastq,3432675258.0,48483287.0,GSM7745907 r1,0:70.80,A:925832958;C:788551205;G:790129536;T:927993012;N:168547,70,,,,925832958,788551205,790129536,927993012,168547,SRX21533022,SRS18745488,SRA1702458,MPI for heart and lung research,MPI for heart and lung research,1,0.75652,,0.04046,,0.76455,,0.46333,,72,,B,,usable mapping rate,illumina,nextseq_v2,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,Germany,2023-08-30,Undetermined,Undetermined,Heart,Cardiovascular System
25323,SRR25810881,SRX21533021,SRS18745487,SRP457576,PRJNA1010780,Effect of egr3 knockout on gene expression of dissected zebrafish hearts,GSE241935,Transcriptome Analysis,To screen for Egr3 targets mediating cardiac valve development we assessed gene expression on dissected hearts of egr3 mutants and wild type siblings. Overall design: To evaluate the transcriptional differences underlying the egr3 mutant phenotype we conducted a bulk RNA seq analysis in dissected zebrafish hearts at 48 hpf. We dissected 20 hearts for each biological duplicate of egr3 mutant and wild type sibling samples in cold DMEM with 10% FBS. Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12µl of RNase free water. Analysis of the obtained reads confirmed the 11bp deletion in the mutant samples. 1. Boezio G. L. M. et al. The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth. Dis Model Mech 16 2023. https://doi.org:10.1242/dmm.049571,,pubmed:38748804,,egr3 mutants 1,GSM7745906,,source name:Heart|tissue:Heart|genotype:bns577 / |geo loc name:missing|collection date:missing,egr3 mutants 1,Trimmomatic version 0.39 was employed to trim reads post a quality drop below a mean of Q20 in a window of 20 nucleotides and keeping only filtered reads longer than 15 nucleotides Reads were aligned versus Ensembl zebrafish genome version danRer11 Ensembl release 104 with STAR 2.7.10a Aligned reads were filtered to remove: duplicates with Picard 2.27.1 Picard: A set of tools in Java for working with next generation sequencing data in the BAM format multi mapping ribosomal or mitochondrial reads. Gene counts were established with featureCounts 2.0.2 by aggregating reads overlapping exons excluding those overlapping multiple genes Liao et al. featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. The raw count matrix was normalized with DESeq2 version 1.30.1 Love et al. Moderated estimation of fold change and dispersion for RNA Seq data with DESeq2 and batch corrected using CountClust each biological replicate = one batch Dey K et al. Visualizing the structure of RNA seq expression data using grade of membership models. Genes were classified as significantly differentially expressed at average count > 5 multiple testing adjusted p value < 0.05 and 0.585 < log2FC > 0.585. Assembly: danRer11 Supplementary files format and content: libryr size notmalize amd batch corrected counts,Heart,,Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12μl of RNase free water 10ng total RNA was used for SMART Seq® v4 Ultra® Low Input RNA Kit Takara Bio.,,tissue:Heart|genotype:bns577 / ,GSM7745906,GSM7745906: egr3 mutants 1; Danio rerio; RNA Seq,GSM7745906 r1,GSM7745906,1,Total RNA was isolated using the miRNeasy micro Kit Qiagen 217084 followed by on column DNase digestion DNase Free DNase Set Qiagen 79254 and final elution was performed in 12μl of RNase free water 10ng total RNA was used for SMART Seq® v4 Ultra® Low Input RNA Kit Takara Bio.,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,NextSeq 2000,,SRP457576,,loader:fastq load.py,E22_3626_Lib_Agatha_egr3_Mut1_R1.fastq.gz,fastq,3838969347.0,54545950.0,GSM7745906 r1,0:70.38,A:1016913415;C:902500250;G:903033204;T:1016194618;N:327860,70,,,,1016913415,902500250,903033204,1016194618,327860,SRX21533021,SRS18745487,SRA1702458,MPI for heart and lung research,MPI for heart and lung research,1,0.9511,,0.05017,,0.75341,,0.47152,,72,,B,,usable mapping rate,illumina,nextseq_v2,unknown,cdna_unspecified,unknown,sc,single_cell_plate,smartseq,,Germany,2023-08-30,Undetermined,Undetermined,Heart,Cardiovascular System
28113,SRR26209648,SRX21920662,SRS19005181,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 3,GSM7812991,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812991,GSM7812991: unDMSO t1 WT 3; Danio rerio; RNA Seq,GSM7812991 r1,GSM7812991,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A30_S48_L005_R1_001.fastq.gz,fastq,616449359.0,6103459.0,GSM7812991 r1,0:101,A:152808626;C:149738763;G:141639387;T:172257280;N:5303,101,,,,152808626,149738763,141639387,172257280,5303,SRX21920662,SRS19005181,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94246,,0.08766,,0.69443,,0.4842,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28114,SRR26209649,SRX21920662,SRS19005181,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 3,GSM7812991,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812991,GSM7812991: unDMSO t1 WT 3; Danio rerio; RNA Seq,GSM7812991 r1,GSM7812991,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A30_S48_L006_R1_001.fastq.gz,fastq,609999297.0,6039597.0,GSM7812991 r2,0:101,A:151226657;C:148143193;G:140088806;T:170530812;N:9829,101,,,,151226657,148143193,140088806,170530812,9829,SRX21920662,SRS19005181,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94201,,0.08569,,0.69572,,0.48692,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28115,SRR26209650,SRX21920662,SRS19005181,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 3,GSM7812991,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812991,GSM7812991: unDMSO t1 WT 3; Danio rerio; RNA Seq,GSM7812991 r1,GSM7812991,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A30_S48_L007_R1_001.fastq.gz,fastq,543730470.0,5383470.0,GSM7812991 r3,0:101,A:134685766;C:132190291;G:124881864;T:151967639;N:4910,101,,,,134685766,132190291,124881864,151967639,4910,SRX21920662,SRS19005181,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.9431,,0.08733,,0.69524,,0.48528,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28116,SRR26209651,SRX21920662,SRS19005181,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 3,GSM7812991,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812991,GSM7812991: unDMSO t1 WT 3; Danio rerio; RNA Seq,GSM7812991 r1,GSM7812991,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A30_S16_L007_R1_001.fastq.gz,fastq,649354149.0,6429249.0,GSM7812991 r4,0:101,A:160756303;C:158145998;G:149623680;T:180816390;N:11778,101,,,,160756303,158145998,149623680,180816390,11778,SRX21920662,SRS19005181,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94438,,0.08618,,0.69473,,0.48809,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28117,SRR26209752,SRX21920662,SRS19005181,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 3,GSM7812991,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812991,GSM7812991: unDMSO t1 WT 3; Danio rerio; RNA Seq,GSM7812991 r1,GSM7812991,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A30_S16_L008_R1_001.fastq.gz,fastq,650450504.0,6440104.0,GSM7812991 r5,0:101,A:161054597;C:158340761;G:149784923;T:181251361;N:18862,101,,,,161054597,158340761,149784923,181251361,18862,SRX21920662,SRS19005181,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94338,,0.08713,,0.6968,,0.48443,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28118,SRR26209652,SRX21920661,SRS19005180,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 2,GSM7812990,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812990,GSM7812990: unDMSO t1 WT 2; Danio rerio; RNA Seq,GSM7812990 r1,GSM7812990,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A29_S46_L005_R1_001.fastq.gz,fastq,593068162.0,5871962.0,GSM7812990 r1,0:101,A:147906639;C:143944406;G:137307101;T:163904929;N:5087,101,,,,147906639,143944406,137307101,163904929,5087,SRX21920661,SRS19005180,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94371,,0.06473,,0.69378,,0.47765,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28119,SRR26209653,SRX21920661,SRS19005180,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 2,GSM7812990,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812990,GSM7812990: unDMSO t1 WT 2; Danio rerio; RNA Seq,GSM7812990 r1,GSM7812990,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A29_S46_L006_R1_001.fastq.gz,fastq,588323081.0,5824981.0,GSM7812990 r2,0:101,A:146630863;C:142829925;G:136124185;T:162728186;N:9922,101,,,,146630863,142829925,136124185,162728186,9922,SRX21920661,SRS19005180,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94282,,0.06357,,0.69418,,0.47665,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28120,SRR26209654,SRX21920661,SRS19005180,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 2,GSM7812990,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812990,GSM7812990: unDMSO t1 WT 2; Danio rerio; RNA Seq,GSM7812990 r1,GSM7812990,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A29_S46_L007_R1_001.fastq.gz,fastq,521265747.0,5161047.0,GSM7812990 r3,0:101,A:129905878;C:126622019;G:120526207;T:144206853;N:4790,101,,,,129905878,126622019,120526207,144206853,4790,SRX21920661,SRS19005180,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94508,,0.06499,,0.69367,,0.4834,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28121,SRR26209655,SRX21920661,SRS19005180,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 2,GSM7812990,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812990,GSM7812990: unDMSO t1 WT 2; Danio rerio; RNA Seq,GSM7812990 r1,GSM7812990,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A29_S26_L007_R1_001.fastq.gz,fastq,623834580.0,6176580.0,GSM7812990 r4,0:101,A:155642981;C:151651242;G:144681951;T:171847192;N:11214,101,,,,155642981,151651242,144681951,171847192,11214,SRX21920661,SRS19005180,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94531,,0.06514,,0.69225,,0.48187,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28122,SRR26209656,SRX21920661,SRS19005180,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 2,GSM7812990,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812990,GSM7812990: unDMSO t1 WT 2; Danio rerio; RNA Seq,GSM7812990 r1,GSM7812990,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A29_S26_L008_R1_001.fastq.gz,fastq,622155960.0,6159960.0,GSM7812990 r5,0:101,A:155145221;C:151231599;G:144290581;T:171470641;N:17918,101,,,,155145221,151231599,144290581,171470641,17918,SRX21920661,SRS19005180,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94319,,0.06413,,0.69449,,0.48159,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28123,SRR26209657,SRX21920660,SRS19005179,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 1,GSM7812989,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812989,GSM7812989: unDMSO t1 WT 1; Danio rerio; RNA Seq,GSM7812989 r1,GSM7812989,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A28_S45_L005_R1_001.fastq.gz,fastq,602520550.0,5965550.0,GSM7812989 r1,0:101,A:149619230;C:146559346;G:139674646;T:166662143;N:5185,101,,,,149619230,146559346,139674646,166662143,5185,SRX21920660,SRS19005179,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94449,,0.06296,,0.69487,,0.48,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28124,SRR26209658,SRX21920660,SRS19005179,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 1,GSM7812989,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812989,GSM7812989: unDMSO t1 WT 1; Danio rerio; RNA Seq,GSM7812989 r1,GSM7812989,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A28_S45_L006_R1_001.fastq.gz,fastq,596942522.0,5910322.0,GSM7812989 r2,0:101,A:148193962;C:145221539;G:138267073;T:165249833;N:10115,101,,,,148193962,145221539,138267073,165249833,10115,SRX21920660,SRS19005179,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.9446,,0.06327,,0.69562,,0.48183,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28125,SRR26209659,SRX21920660,SRS19005179,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 1,GSM7812989,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812989,GSM7812989: unDMSO t1 WT 1; Danio rerio; RNA Seq,GSM7812989 r1,GSM7812989,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A28_S45_L007_R1_001.fastq.gz,fastq,532539064.0,5272664.0,GSM7812989 r3,0:101,A:132217323;C:129660296;G:123303893;T:147352732;N:4820,101,,,,132217323,129660296,123303893,147352732,4820,SRX21920660,SRS19005179,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94579,,0.06237,,0.69554,,0.46658,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28126,SRR26209660,SRX21920660,SRS19005179,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 1,GSM7812989,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812989,GSM7812989: unDMSO t1 WT 1; Danio rerio; RNA Seq,GSM7812989 r1,GSM7812989,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A28_S29_L007_R1_001.fastq.gz,fastq,633337569.0,6270669.0,GSM7812989 r4,0:101,A:157286526;C:154336036;G:147137950;T:174565295;N:11762,101,,,,157286526,154336036,147137950,174565295,11762,SRX21920660,SRS19005179,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94562,,0.06294,,0.69489,,0.47406,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28127,SRR26209661,SRX21920660,SRS19005179,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 WT 1,GSM7812989,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 WT 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:WT|treatment:unDMSO,GSM7812989,GSM7812989: unDMSO t1 WT 1; Danio rerio; RNA Seq,GSM7812989 r1,GSM7812989,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A28_S29_L008_R1_001.fastq.gz,fastq,636051540.0,6297540.0,GSM7812989 r5,0:101,A:157845668;C:154972063;G:147765532;T:175449801;N:18476,101,,,,157845668,154972063,147765532,175449801,18476,SRX21920660,SRS19005179,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.9471,,0.06288,,0.69536,,0.4797,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28128,SRR26209662,SRX21920659,SRS19005178,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 3,GSM7812988,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812988,GSM7812988: unDMSO t1 K2 3; Danio rerio; RNA Seq,GSM7812988 r1,GSM7812988,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A36_S54_L005_R1_001.fastq.gz,fastq,674188635.0,6675135.0,GSM7812988 r1,0:101,A:173104307;C:161481671;G:152542615;T:187053812;N:6230,101,,,,173104307,161481671,152542615,187053812,6230,SRX21920659,SRS19005178,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94039,,0.07795,,0.69877,,0.48386,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28129,SRR26209663,SRX21920659,SRS19005178,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 3,GSM7812988,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812988,GSM7812988: unDMSO t1 K2 3; Danio rerio; RNA Seq,GSM7812988 r1,GSM7812988,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A36_S54_L006_R1_001.fastq.gz,fastq,667048339.0,6604439.0,GSM7812988 r2,0:101,A:171316049;C:159786547;G:150743446;T:185190228;N:12069,101,,,,171316049,159786547,150743446,185190228,12069,SRX21920659,SRS19005178,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94064,,0.07881,,0.69767,,0.48162,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28130,SRR26209664,SRX21920659,SRS19005178,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 3,GSM7812988,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812988,GSM7812988: unDMSO t1 K2 3; Danio rerio; RNA Seq,GSM7812988 r1,GSM7812988,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A36_S54_L007_R1_001.fastq.gz,fastq,596645683.0,5907383.0,GSM7812988 r3,0:101,A:153131508;C:143126741;G:134745614;T:165635953;N:5867,101,,,,153131508,143126741,134745614,165635953,5867,SRX21920659,SRS19005178,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94156,,0.07795,,0.69751,,0.4833,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28131,SRR26209665,SRX21920659,SRS19005178,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 3,GSM7812988,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812988,GSM7812988: unDMSO t1 K2 3; Danio rerio; RNA Seq,GSM7812988 r1,GSM7812988,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A36_S12_L007_R1_001.fastq.gz,fastq,712385926.0,7053326.0,GSM7812988 r4,0:101,A:183100436;C:171026605;G:161510527;T:196734608;N:13750,101,,,,183100436,171026605,161510527,196734608,13750,SRX21920659,SRS19005178,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94298,,0.07892,,0.6997,,0.48331,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28132,SRR26209666,SRX21920659,SRS19005178,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 3,GSM7812988,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812988,GSM7812988: unDMSO t1 K2 3; Danio rerio; RNA Seq,GSM7812988 r1,GSM7812988,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A36_S12_L008_R1_001.fastq.gz,fastq,714661355.0,7075855.0,GSM7812988 r5,0:101,A:183454698;C:171554090;G:162064705;T:197567065;N:20797,101,,,,183454698,171554090,162064705,197567065,20797,SRX21920659,SRS19005178,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94316,,0.07877,,0.70051,,0.48354,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28133,SRR26209667,SRX21920658,SRS19005177,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 2,GSM7812987,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812987,GSM7812987: unDMSO t1 K2 2; Danio rerio; RNA Seq,GSM7812987 r1,GSM7812987,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A35_S53_L005_R1_001.fastq.gz,fastq,611900723.0,6058423.0,GSM7812987 r1,0:101,A:154668392;C:147817141;G:139653400;T:169756342;N:5448,101,,,,154668392,147817141,139653400,169756342,5448,SRX21920658,SRS19005177,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94636,,0.08022,,0.69962,,0.48201,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28134,SRR26209668,SRX21920658,SRS19005177,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 2,GSM7812987,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812987,GSM7812987: unDMSO t1 K2 2; Danio rerio; RNA Seq,GSM7812987 r1,GSM7812987,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A35_S53_L006_R1_001.fastq.gz,fastq,602971818.0,5970018.0,GSM7812987 r2,0:101,A:152346877;C:145640105;G:137576284;T:167398281;N:10271,101,,,,152346877,145640105,137576284,167398281,10271,SRX21920658,SRS19005177,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94549,,0.08086,,0.69781,,0.47842,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28135,SRR26209669,SRX21920658,SRS19005177,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 2,GSM7812987,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812987,GSM7812987: unDMSO t1 K2 2; Danio rerio; RNA Seq,GSM7812987 r1,GSM7812987,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A35_S53_L007_R1_001.fastq.gz,fastq,539246575.0,5339075.0,GSM7812987 r3,0:101,A:136317506;C:130390122;G:122927887;T:149606028;N:5032,101,,,,136317506,130390122,122927887,149606028,5032,SRX21920658,SRS19005177,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94653,,0.08075,,0.69704,,0.48374,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28136,SRR26209670,SRX21920658,SRS19005177,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 2,GSM7812987,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812987,GSM7812987: unDMSO t1 K2 2; Danio rerio; RNA Seq,GSM7812987 r1,GSM7812987,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A35_S47_L007_R1_001.fastq.gz,fastq,643197593.0,6368293.0,GSM7812987 r4,0:101,A:162743675;C:155711536;G:147135700;T:177594459;N:12223,101,,,,162743675,155711536,147135700,177594459,12223,SRX21920658,SRS19005177,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94757,,0.08023,,0.70055,,0.48458,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28137,SRR26209671,SRX21920658,SRS19005177,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 2,GSM7812987,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812987,GSM7812987: unDMSO t1 K2 2; Danio rerio; RNA Seq,GSM7812987 r1,GSM7812987,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A35_S47_L008_R1_001.fastq.gz,fastq,644356063.0,6379763.0,GSM7812987 r5,0:101,A:162915651;C:155935181;G:147406641;T:178079118;N:19472,101,,,,162915651,155935181,147406641,178079118,19472,SRX21920658,SRS19005177,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94817,,0.08218,,0.70017,,0.48286,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28138,SRR26209672,SRX21920657,SRS19005176,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 1,GSM7812986,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812986,GSM7812986: unDMSO t1 K2 1; Danio rerio; RNA Seq,GSM7812986 r1,GSM7812986,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A34_S52_L005_R1_001.fastq.gz,fastq,615996374.0,6098974.0,GSM7812986 r1,0:101,A:156934542;C:147417998;G:139301915;T:172336421;N:5498,101,,,,156934542,147417998,139301915,172336421,5498,SRX21920657,SRS19005176,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94179,,0.07628,,0.69512,,0.48041,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28139,SRR26209673,SRX21920657,SRS19005176,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 1,GSM7812986,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812986,GSM7812986: unDMSO t1 K2 1; Danio rerio; RNA Seq,GSM7812986 r1,GSM7812986,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A34_S52_L006_R1_001.fastq.gz,fastq,608875571.0,6028471.0,GSM7812986 r2,0:101,A:155127967;C:145701864;G:137575885;T:170459017;N:10838,101,,,,155127967,145701864,137575885,170459017,10838,SRX21920657,SRS19005176,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94248,,0.07563,,0.69512,,0.47982,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28140,SRR26209674,SRX21920657,SRS19005176,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 1,GSM7812986,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812986,GSM7812986: unDMSO t1 K2 1; Danio rerio; RNA Seq,GSM7812986 r1,GSM7812986,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A34_S52_L007_R1_001.fastq.gz,fastq,539739455.0,5343955.0,GSM7812986 r3,0:101,A:137431217;C:129328124;G:121891472;T:151083373;N:5269,101,,,,137431217,129328124,121891472,151083373,5269,SRX21920657,SRS19005176,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.9438,,0.07511,,0.69654,,0.48083,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28141,SRR26209675,SRX21920657,SRS19005176,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 1,GSM7812986,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812986,GSM7812986: unDMSO t1 K2 1; Danio rerio; RNA Seq,GSM7812986 r1,GSM7812986,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A34_S65_L007_R1_001.fastq.gz,fastq,648984489.0,6425589.0,GSM7812986 r4,0:101,A:165552674;C:155580501;G:147078092;T:180761105;N:12117,101,,,,165552674,155580501,147078092,180761105,12117,SRX21920657,SRS19005176,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94513,,0.07694,,0.69451,,0.48174,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28142,SRR26209676,SRX21920657,SRS19005176,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K2 1,GSM7812986,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K2 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K2|treatment:unDMSO,GSM7812986,GSM7812986: unDMSO t1 K2 1; Danio rerio; RNA Seq,GSM7812986 r1,GSM7812986,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A34_S65_L008_R1_001.fastq.gz,fastq,651988330.0,6455330.0,GSM7812986 r5,0:101,A:166130853;C:156282200;G:147738788;T:181817695;N:18794,101,,,,166130853,156282200,147738788,181817695,18794,SRX21920657,SRS19005176,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94437,,0.0759,,0.69597,,0.48083,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28143,SRR26209677,SRX21920656,SRS19005175,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 3,GSM7812985,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812985,GSM7812985: unDMSO t1 K1 3; Danio rerio; RNA Seq,GSM7812985 r1,GSM7812985,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A33_S51_L005_R1_001.fastq.gz,fastq,621669544.0,6155144.0,GSM7812985 r1,0:101,A:157346917;C:148083763;G:141122336;T:175110946;N:5582,101,,,,157346917,148083763,141122336,175110946,5582,SRX21920656,SRS19005175,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94207,,0.06859,,0.69473,,0.48838,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28144,SRR26209678,SRX21920656,SRS19005175,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 3,GSM7812985,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812985,GSM7812985: unDMSO t1 K1 3; Danio rerio; RNA Seq,GSM7812985 r1,GSM7812985,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A33_S51_L006_R1_001.fastq.gz,fastq,613437842.0,6073642.0,GSM7812985 r2,0:101,A:155200714;C:146135271;G:139223090;T:172868018;N:10749,101,,,,155200714,146135271,139223090,172868018,10749,SRX21920656,SRS19005175,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94144,,0.06885,,0.69471,,0.48996,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28145,SRR26209679,SRX21920656,SRS19005175,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 3,GSM7812985,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812985,GSM7812985: unDMSO t1 K1 3; Danio rerio; RNA Seq,GSM7812985 r1,GSM7812985,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A33_S51_L007_R1_001.fastq.gz,fastq,546684518.0,5412718.0,GSM7812985 r3,0:101,A:138292090;C:130364936;G:123978492;T:154043685;N:5315,101,,,,138292090,130364936,123978492,154043685,5315,SRX21920656,SRS19005175,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94375,,0.06988,,0.69589,,0.48741,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28146,SRR26209680,SRX21920656,SRS19005175,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 3,GSM7812985,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812985,GSM7812985: unDMSO t1 K1 3; Danio rerio; RNA Seq,GSM7812985 r1,GSM7812985,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A33_S43_L007_R1_001.fastq.gz,fastq,651065089.0,6446189.0,GSM7812985 r4,0:101,A:164874330;C:155389435;G:148130531;T:182658473;N:12320,101,,,,164874330,155389435,148130531,182658473,12320,SRX21920656,SRS19005175,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94247,,0.06954,,0.69538,,0.48283,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28147,SRR26209681,SRX21920656,SRS19005175,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 3,GSM7812985,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812985,GSM7812985: unDMSO t1 K1 3; Danio rerio; RNA Seq,GSM7812985 r1,GSM7812985,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A33_S43_L008_R1_001.fastq.gz,fastq,652838952.0,6463752.0,GSM7812985 r5,0:101,A:165235507;C:155756581;G:148483290;T:183344035;N:19539,101,,,,165235507,155756581,148483290,183344035,19539,SRX21920656,SRS19005175,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94297,,0.06917,,0.69593,,0.4827,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28148,SRR26209682,SRX21920655,SRS19005174,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 2,GSM7812984,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812984,GSM7812984: unDMSO t1 K1 2; Danio rerio; RNA Seq,GSM7812984 r1,GSM7812984,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A32_S50_L005_R1_001.fastq.gz,fastq,531938215.0,5266715.0,GSM7812984 r1,0:101,A:131083905;C:128715617;G:122601298;T:149532970;N:4425,101,,,,131083905,128715617,122601298,149532970,4425,SRX21920655,SRS19005174,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94249,,0.07108,,0.69485,,0.47386,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28149,SRR26209683,SRX21920655,SRS19005174,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 2,GSM7812984,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812984,GSM7812984: unDMSO t1 K1 2; Danio rerio; RNA Seq,GSM7812984 r1,GSM7812984,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A32_S50_L006_R1_001.fastq.gz,fastq,527781863.0,5225563.0,GSM7812984 r2,0:101,A:130119726;C:127646784;G:121549831;T:148456993;N:8529,101,,,,130119726,127646784,121549831,148456993,8529,SRX21920655,SRS19005174,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94199,,0.0713,,0.69337,,0.473,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28150,SRR26209684,SRX21920655,SRS19005174,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 2,GSM7812984,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812984,GSM7812984: unDMSO t1 K1 2; Danio rerio; RNA Seq,GSM7812984 r1,GSM7812984,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A32_S50_L007_R1_001.fastq.gz,fastq,472785040.0,4681040.0,GSM7812984 r3,0:101,A:116503463;C:114489771;G:108989839;T:132797698;N:4269,101,,,,116503463,114489771,108989839,132797698,4269,SRX21920655,SRS19005174,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94373,,0.07285,,0.69467,,0.47913,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28151,SRR26209685,SRX21920655,SRS19005174,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 2,GSM7812984,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812984,GSM7812984: unDMSO t1 K1 2; Danio rerio; RNA Seq,GSM7812984 r1,GSM7812984,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A32_S71_L007_R1_001.fastq.gz,fastq,561056414.0,5555014.0,GSM7812984 r4,0:101,A:138240115;C:136040813;G:129592563;T:157172399;N:10524,101,,,,138240115,136040813,129592563,157172399,10524,SRX21920655,SRS19005174,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94357,,0.07109,,0.69544,,0.47169,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28152,SRR26209686,SRX21920655,SRS19005174,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 2,GSM7812984,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812984,GSM7812984: unDMSO t1 K1 2; Danio rerio; RNA Seq,GSM7812984 r1,GSM7812984,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A32_S71_L008_R1_001.fastq.gz,fastq,563109744.0,5575344.0,GSM7812984 r5,0:101,A:138778764;C:136444809;G:129964766;T:157904708;N:16697,101,,,,138778764,136444809,129964766,157904708,16697,SRX21920655,SRS19005174,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94358,,0.07253,,0.69345,,0.47443,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28153,SRR26209687,SRX21920654,SRS19005173,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 1,GSM7812983,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812983,GSM7812983: unDMSO t1 K1 1; Danio rerio; RNA Seq,GSM7812983 r1,GSM7812983,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A31_S49_L005_R1_001.fastq.gz,fastq,576725857.0,5710157.0,GSM7812983 r1,0:101,A:142557020;C:140092748;G:133378036;T:160693137;N:4916,101,,,,142557020,140092748,133378036,160693137,4916,SRX21920654,SRS19005173,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94239,,0.07259,,0.69398,,0.47675,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28154,SRR26209688,SRX21920654,SRS19005173,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 1,GSM7812983,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812983,GSM7812983: unDMSO t1 K1 1; Danio rerio; RNA Seq,GSM7812983 r1,GSM7812983,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A31_S49_L006_R1_001.fastq.gz,fastq,569166310.0,5635310.0,GSM7812983 r2,0:101,A:140702145;C:138232540;G:131558917;T:158663414;N:9294,101,,,,140702145,138232540,131558917,158663414,9294,SRX21920654,SRS19005173,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94221,,0.07252,,0.69524,,0.4833,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28155,SRR26209689,SRX21920654,SRS19005173,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 1,GSM7812983,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812983,GSM7812983: unDMSO t1 K1 1; Danio rerio; RNA Seq,GSM7812983 r1,GSM7812983,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A31_S49_L007_R1_001.fastq.gz,fastq,505699425.0,5006925.0,GSM7812983 r3,0:101,A:124940125;C:122891114;G:116919354;T:140944307;N:4525,101,,,,124940125,122891114,116919354,140944307,4525,SRX21920654,SRS19005173,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.944,,0.07242,,0.69473,,0.48289,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28156,SRR26209690,SRX21920654,SRS19005173,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 1,GSM7812983,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812983,GSM7812983: unDMSO t1 K1 1; Danio rerio; RNA Seq,GSM7812983 r1,GSM7812983,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A31_S27_L007_R1_001.fastq.gz,fastq,598624374.0,5926974.0,GSM7812983 r4,0:101,A:147863525;C:145723385;G:138724140;T:166302217;N:11107,101,,,,147863525,145723385,138724140,166302217,11107,SRX21920654,SRS19005173,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94426,,0.07312,,0.69479,,0.48056,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28157,SRR26209691,SRX21920654,SRS19005173,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,unDMSO t1 K1 1,GSM7812983,,source name:whole embryo|tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO|geo loc name:missing|collection date:missing,unDMSO t1 K1 1,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t1|genotype:K1|treatment:unDMSO,GSM7812983,GSM7812983: unDMSO t1 K1 1; Danio rerio; RNA Seq,GSM7812983 r1,GSM7812983,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A31_S27_L008_R1_001.fastq.gz,fastq,602654072.0,5966872.0,GSM7812983 r5,0:101,A:148869054;C:146693906;G:139571739;T:167501958;N:17415,101,,,,148869054,146693906,139571739,167501958,17415,SRX21920654,SRS19005173,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.9433,,0.0732,,0.69552,,0.47807,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28158,SRR26209692,SRX21920653,SRS19005172,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 3,GSM7812982,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812982,GSM7812982: IR t2 WT 3; Danio rerio; RNA Seq,GSM7812982 r1,GSM7812982,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A21_S38_L005_R1_001.fastq.gz,fastq,617990619.0,6118719.0,GSM7812982 r1,0:101,A:155764078;C:148642484;G:140551460;T:173027146;N:5451,101,,,,155764078,148642484,140551460,173027146,5451,SRX21920653,SRS19005172,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.93982,,0.07316,,0.68801,,0.48964,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28159,SRR26209693,SRX21920653,SRS19005172,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 3,GSM7812982,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812982,GSM7812982: IR t2 WT 3; Danio rerio; RNA Seq,GSM7812982 r1,GSM7812982,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A21_S38_L006_R1_001.fastq.gz,fastq,612677312.0,6066112.0,GSM7812982 r2,0:101,A:154425053;C:147379484;G:139208932;T:171653577;N:10266,101,,,,154425053,147379484,139208932,171653577,10266,SRX21920653,SRS19005172,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.93954,,0.07439,,0.68945,,0.48768,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28160,SRR26209694,SRX21920653,SRS19005172,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 3,GSM7812982,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812982,GSM7812982: IR t2 WT 3; Danio rerio; RNA Seq,GSM7812982 r1,GSM7812982,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A21_S38_L007_R1_001.fastq.gz,fastq,545311221.0,5399121.0,GSM7812982 r3,0:101,A:137422024;C:131281461;G:123803444;T:152799164;N:5128,101,,,,137422024,131281461,123803444,152799164,5128,SRX21920653,SRS19005172,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94119,,0.07433,,0.6886,,0.48763,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28161,SRR26209695,SRX21920653,SRS19005172,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 3,GSM7812982,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812982,GSM7812982: IR t2 WT 3; Danio rerio; RNA Seq,GSM7812982 r1,GSM7812982,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A21_S68_L007_R1_001.fastq.gz,fastq,643233246.0,6368646.0,GSM7812982 r4,0:101,A:162062613;C:155064589;G:146625670;T:179468362;N:12012,101,,,,162062613,155064589,146625670,179468362,12012,SRX21920653,SRS19005172,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94076,,0.07335,,0.68747,,0.48953,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28162,SRR26209696,SRX21920653,SRS19005172,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 3,GSM7812982,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 3,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812982,GSM7812982: IR t2 WT 3; Danio rerio; RNA Seq,GSM7812982 r1,GSM7812982,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A21_S68_L008_R1_001.fastq.gz,fastq,643010339.0,6366439.0,GSM7812982 r5,0:101,A:161939065;C:154897858;G:146526716;T:179627762;N:18938,101,,,,161939065,154897858,146526716,179627762,18938,SRX21920653,SRS19005172,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94077,,0.07428,,0.68852,,0.4878,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28163,SRR26209697,SRX21920652,SRS19005171,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 2,GSM7812981,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812981,GSM7812981: IR t2 WT 2; Danio rerio; RNA Seq,GSM7812981 r1,GSM7812981,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A20_S37_L005_R1_001.fastq.gz,fastq,608303002.0,6022802.0,GSM7812981 r1,0:101,A:151266125;C:146608795;G:139308397;T:171114479;N:5206,101,,,,151266125,146608795,139308397,171114479,5206,SRX21920652,SRS19005171,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.9409,,0.06934,,0.68702,,0.48691,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28164,SRR26209698,SRX21920652,SRS19005171,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 2,GSM7812981,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812981,GSM7812981: IR t2 WT 2; Danio rerio; RNA Seq,GSM7812981 r1,GSM7812981,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A20_S37_L006_R1_001.fastq.gz,fastq,602278857.0,5963157.0,GSM7812981 r2,0:101,A:149885037;C:145060278;G:137831227;T:169492101;N:10214,101,,,,149885037,145060278,137831227,169492101,10214,SRX21920652,SRS19005171,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94168,,0.06967,,0.68491,,0.48684,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28165,SRR26209699,SRX21920652,SRS19005171,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 2,GSM7812981,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812981,GSM7812981: IR t2 WT 2; Danio rerio; RNA Seq,GSM7812981 r1,GSM7812981,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A20_S37_L007_R1_001.fastq.gz,fastq,539699863.0,5343563.0,GSM7812981 r3,0:101,A:134101752;C:130207416;G:123553593;T:151832349;N:4753,101,,,,134101752,130207416,123553593,151832349,4753,SRX21920652,SRS19005171,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94262,,0.06995,,0.68605,,0.48097,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures
28166,SRR26209700,SRX21920652,SRS19005171,SRP463749,PRJNA1022096,Zebrafish reveal new roles for Fam83f in hatching and DNA damage mediated autophagic responses,GSE244291,Transcriptome Analysis,The FAM83 Family with sequence similarity 83 family is highly conserved in vertebrates yet little is known of the functions of these proteins beyond a correlation with oncogenesis. Of the family FAM83F is of particular interest because it is the only membrane targeted FAM83 protein. FAM83F has been shown to activate the canonical Wnt signalling pathway and bind to and stabilize p53 when overexpressed two pathways often dysregulated in disease. Insights into gene function can often be gained by studying the roles they play during development and here we report the generation of fam83f knock out fam83f / zebrafish which we have used to elucidate the role of Fam83f in vivo. We show that endogenous fam83f is most strongly expressed in the proteolytic enzyme containing hatch gland of developing zebrafish embryos and that fam83f / embryos hatch earlier than WT counterparts despite developing at a comparable temporal rate. We demonstrate that fam83f / embryos are more sensitive to ionizing radiation than WT embryos a finding that contrasts with the previously reported role of FAM83F as a stabilizer of p53. Transcriptomic analysis shows that loss of fam83f causes downregulation of phosphatidylinositol 3 phosphate PI3P binding proteins and impairment of cellular degradation pathways particularly autophagy which is a crucial component of the DNA damage response. Finally we show that Fam83f protein is itself targeted to the lysosome when expressed in cultured cells and that this localization is dependent upon a C' terminal signal sequence. The zebrafish lines we have generated here suggest for the first time that Fam83f plays an important role in autophagic/lysosomal processes resulting in dysregulated hatching and increased sensitivity to genotoxic stress in vivo. Overall design: fam83fa / zebrafish are more sensitive to ionizing radiation IR than WT counterparts. We conducted bulk RNA seq on WT vs two different fam83fa / K1 and K2 zebrafish lines by subjecting 24 hpf embryos from each genotype to IR then extracting total RNA at 2 and 10 hours following treament t1 and t2. Three biological replicates of a minimum of 10 embryos per replicate were sequenced by bulk RNA seq to to identify any differences in the DNA damage response between WT and fam83fa / mutants. Treatments: AD = Actinomycin D in DMSO vehicle IR = ionizing radiation 20 Grays gamma radiation unDMSO = untreated AD control vehicle only i.e. DMSO un = untreated IR control,,pubmed:39437839,,IR t2 WT 2,GSM7812981,,source name:whole embryo|tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR|geo loc name:missing|collection date:missing,IR t2 WT 2,Cutadapt 1.9.1 rsem 1.3.0 star 2.5.2a Assembly: GRCz11 assembly Supplementary files format and content: *.genes.results are rsem count files,whole embryo,Embryos were exposed to gamma IR of 20 Gy at xxx hpf,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,Zebrafish embryos post collection were maintained at 28.5C in E2 medium at a density ⋜ 50 embryos,tissue:whole embryo|timepoint:t2|genotype:WT|treatment:IR,GSM7812981,GSM7812981: IR t2 WT 2; Danio rerio; RNA Seq,GSM7812981 r1,GSM7812981,1,Total RNA was extracted using RNeasy Mini Kit QIAGEN rRNA depletion Rio Zero Plus rRNA Depletion Kit Ilumina then library prep using KAPA mRNA HyperPrep Kit for Ilumina Platforms,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 4000,,SRP463749,,loader:fastq load.py,JON686A20_S13_L007_R1_001.fastq.gz,fastq,629529263.0,6232963.0,GSM7812981 r4,0:101,A:156345013;C:152106432;G:144536894;T:176529480;N:11444,101,,,,156345013,152106432,144536894,176529480,11444,SRX21920652,SRS19005171,SRA1722794,"Devenport, Molecular Biology, Princeton University","Devenport, Molecular Biology, Princeton University",1,0.94267,,0.06864,,0.68477,,0.47912,,101,,B,,usable mapping rate,illumina,hiseq_era,unknown,rrna_depletion,unknown,bulk,bulk,bulk,,United States,2023-09-28,Undetermined,Embryo,Whole Organism,All anatomical structures