rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
11226,ERR10368639,ERX9900562,ERS13563108,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X9,SAMEA111469072,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469072|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X9|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:snrnp70  / |organism part:whole organism|sample name:E MTAB 12301:16261X9,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X9 p,16261X9 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:snrnp70  / ,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,16261X9_190815_A00421_0101_BHFL23DRXX_S2_L001_R1_001.fastq.gz 16261X9_190815_A00421_0101_BHFL23DRXX_S2_L001_R2_001.fastq.gz,fastq fastq,2686590240.0,26339120.0,E MTAB 12301:16261X9 190815 A00421 0101 BHFL23DRXX S2 L001 R,0:51 1:51,A:694597606;C:635153638;G:628248232;T:728333026;N:257738,51,51,,,694597606,635153638,628248232,728333026,257738,ERX9900562,ERS13563108,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.94614,0.94723,0.1538,0.15109,0.70398,0.70352,0.48123,0.48168,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11227,ERR10368638,ERX9900561,ERS13563107,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X8,SAMEA111469071,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469071|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X8|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:snrnp70  / |organism part:whole organism|sample name:E MTAB 12301:16261X8,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X8 p,16261X8 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:snrnp70  / ,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,16261X8_190815_A00421_0101_BHFL23DRXX_S4_L001_R1_001.fastq.gz 16261X8_190815_A00421_0101_BHFL23DRXX_S4_L001_R2_001.fastq.gz,fastq fastq,15003930006.0,147097353.0,E MTAB 12301:16261X8 190815 A00421 0101 BHFL23DRXX S4 L001 R,0:51 1:51,A:3882939949;C:3570150556;G:3520942177;T:4028462946;N:1434378,51,51,,,3882939949,3570150556,3520942177,4028462946,1434378,ERX9900561,ERS13563107,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.94373,0.94762,0.17954,0.17832,0.70299,0.70207,0.48822,0.48842,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11228,ERR10368637,ERX9900560,ERS13563106,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X7,SAMEA111469070,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469070|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X7|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:snrnp70  / |organism part:whole organism|sample name:E MTAB 12301:16261X7,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X7 p,16261X7 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:snrnp70  / ,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|loader:fastq load.py,16261X7_190815_A00421_0101_BHFL23DRXX_S6_L001_R1_001.fastq.gz 16261X7_190815_A00421_0101_BHFL23DRXX_S6_L001_R2_001.fastq.gz,fastq fastq,3806643060.0,37320030.0,E MTAB 12301:16261X7 190815 A00421 0101 BHFL23DRXX S6 L001 R,0:51 1:51,A:971582421;C:919313664;G:905728446;T:1009653865;N:364664,51,51,,,971582421,919313664,905728446,1009653865,364664,ERX9900560,ERS13563106,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.94035,0.94356,0.1661,0.16393,0.70136,0.70082,0.48294,0.48049,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11229,ERR10368636,ERX9900559,ERS13563105,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X6,SAMEA111469069,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469069|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X6|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:+/cyt hSNRNP70|organism part:whole organism|sample name:E MTAB 12301:16261X6,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X6 p,16261X6 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:+/cyt hSNRNP70,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,16261X6_190815_A00421_0101_BHFL23DRXX_S7_L001_R1_001.fastq.gz 16261X6_190815_A00421_0101_BHFL23DRXX_S7_L001_R2_001.fastq.gz,fastq fastq,2875874598.0,28194849.0,E MTAB 12301:16261X6 190815 A00421 0101 BHFL23DRXX S7 L001 R,0:51 1:51,A:745870043;C:683782229;G:673037324;T:772909720;N:275282,51,51,,,745870043,683782229,673037324,772909720,275282,ERX9900559,ERS13563105,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.9435,0.94737,0.16987,0.16888,0.6983,0.69826,0.47122,0.47409,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11230,ERR10368635,ERX9900558,ERS13563104,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X5,SAMEA111469068,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469068|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X5|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:+/cyt hSNRNP70|organism part:whole organism|sample name:E MTAB 12301:16261X5,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X5 p,16261X5 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:+/cyt hSNRNP70,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,16261X5_190815_A00421_0101_BHFL23DRXX_S8_L001_R1_001.fastq.gz 16261X5_190815_A00421_0101_BHFL23DRXX_S8_L001_R2_001.fastq.gz,fastq fastq,2656716072.0,26046236.0,E MTAB 12301:16261X5 190815 A00421 0101 BHFL23DRXX S8 L001 R,0:51 1:51,A:693979046;C:627484571;G:617505105;T:717493108;N:254242,51,51,,,693979046,627484571,617505105,717493108,254242,ERX9900558,ERS13563104,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.94387,0.94796,0.16299,0.16183,0.69556,0.69581,0.46842,0.47334,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11231,ERR10368634,ERX9900557,ERS13563103,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X4,SAMEA111469067,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469067|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X4|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:+/cyt hSNRNP70|organism part:whole organism|sample name:E MTAB 12301:16261X4,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X4 p,16261X4 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:+/cyt hSNRNP70,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,16261X4_190815_A00421_0101_BHFL23DRXX_S9_L001_R1_001.fastq.gz 16261X4_190815_A00421_0101_BHFL23DRXX_S9_L001_R2_001.fastq.gz,fastq fastq,2693177298.0,26403699.0,E MTAB 12301:16261X4 190815 A00421 0101 BHFL23DRXX S9 L001 R,0:51 1:51,A:698547378;C:640687214;G:628869192;T:724816757;N:256757,51,51,,,698547378,640687214,628869192,724816757,256757,ERX9900557,ERS13563103,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.94101,0.94526,0.17344,0.17201,0.68619,0.68513,0.47366,0.4702,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11232,ERR10368633,ERX9900556,ERS13563102,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X3,SAMEA111469066,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469066|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X3|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:wild type genotype|organism part:whole organism|sample name:E MTAB 12301:16261X3,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X3 p,16261X3 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|loader:fastq load.py|options:  dnus,16261X3_190815_A00421_0101_BHFL23DRXX_S10_L001_R1_001.fastq.gz 16261X3_190815_A00421_0101_BHFL23DRXX_S10_L001_R2_001.fastq.gz,fastq fastq,3104796462.0,60878362.0,E MTAB 12301:16261X3 190815 A00421 0101 BHFL23DRXX S10 L001 R,0:51,A:803595677;C:737898133;G:731641815;T:831364716;N:296121,51,,,,803595677,737898133,731641815,831364716,296121,ERX9900556,ERS13563102,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,1,0.94361,,0.16161,,0.69822,,0.4647,,51,,B,,usable mapping rate,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11233,ERR10368632,ERX9900555,ERS13563101,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X2,SAMEA111469065,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469065|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X2|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:wild type genotype|organism part:whole organism|sample name:E MTAB 12301:16261X2,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X2 p,16261X2 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,16261X2_190815_A00421_0101_BHFL23DRXX_S11_L001_R1_001.fastq.gz 16261X2_190815_A00421_0101_BHFL23DRXX_S11_L001_R2_001.fastq.gz,fastq fastq,2935625892.0,28780646.0,E MTAB 12301:16261X2 190815 A00421 0101 BHFL23DRXX S11 L001 R,0:51 1:51,A:759844700;C:699591057;G:694530268;T:781380766;N:279101,51,51,,,759844700,699591057,694530268,781380766,279101,ERX9900555,ERS13563101,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.93297,0.93744,0.16748,0.16566,0.69929,0.6996,0.4788,0.47875,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11234,ERR10368631,ERX9900554,ERS13563100,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X12,SAMEA111469064,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469064|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:52Z|INSDC last update:2022 10 31T00:16:52Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X12|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:cyt hSNRNP70/ |organism part:whole organism|sample name:E MTAB 12301:16261X12,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X12 p,16261X12 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:cyt hSNRNP70/ ,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,16261X12_190815_A00421_0101_BHFL23DRXX_S3_L001_R1_001.fastq.gz 16261X12_190815_A00421_0101_BHFL23DRXX_S3_L001_R2_001.fastq.gz,fastq fastq,7451469240.0,73053620.0,E MTAB 12301:16261X12 190815 A00421 0101 BHFL23DRXX S3 L001 R,0:51 1:51,A:1950856364;C:1747213644;G:1718730947;T:2033957057;N:711228,51,51,,,1950856364,1747213644,1718730947,2033957057,711228,ERX9900554,ERS13563100,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.9372,0.94118,0.20267,0.19972,0.69794,0.69735,0.47505,0.4883,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11235,ERR10368630,ERX9900553,ERS13563099,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X11,SAMEA111469063,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469063|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:51Z|INSDC last update:2022 10 31T00:16:51Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X11|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:cyt hSNRNP70/ |organism part:whole organism|sample name:E MTAB 12301:16261X11,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X11 p,16261X11 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:cyt hSNRNP70/ ,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,16261X11_190815_A00421_0101_BHFL23DRXX_S5_L001_R1_001.fastq.gz 16261X11_190815_A00421_0101_BHFL23DRXX_S5_L001_R2_001.fastq.gz,fastq fastq,2754589458.0,27005779.0,E MTAB 12301:16261X11 190815 A00421 0101 BHFL23DRXX S5 L001 R,0:51 1:51,A:715675810;C:652231961;G:640938288;T:745480460;N:262939,51,51,,,715675810,652231961,640938288,745480460,262939,ERX9900553,ERS13563099,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.93802,0.94247,0.17937,0.17728,0.70195,0.70067,0.48458,0.48364,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11236,ERR10368629,ERX9900552,ERS13563098,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X10,SAMEA111469062,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469062|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:51Z|INSDC last update:2022 10 31T00:16:51Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X10|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:cyt hSNRNP70/ |organism part:whole organism|sample name:E MTAB 12301:16261X10,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X10 p,16261X10 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:cyt hSNRNP70/ ,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|loader:fastq load.py,16261X10_190815_A00421_0101_BHFL23DRXX_S1_L001_R1_001.fastq.gz 16261X10_190815_A00421_0101_BHFL23DRXX_S1_L001_R2_001.fastq.gz,fastq fastq,2319764682.0,22742791.0,E MTAB 12301:16261X10 190815 A00421 0101 BHFL23DRXX S1 L001 R,0:51 1:51,A:596937200;C:555502273;G:546190804;T:620912915;N:221490,51,51,,,596937200,555502273,546190804,620912915,221490,ERX9900552,ERS13563098,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.94034,0.94451,0.1621,0.16043,0.69406,0.6928,0.4936,0.49757,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
11237,ERR10368628,ERX9900551,ERS13563097,ERP141667,PRJEB56699,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E-MTAB-12301,Other,It has been recently shown that SNRNP70  a major component of the spliceosome  as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons  we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic overexpression of cytoplasmic only human SNRNP70 cyt hSNRNP70. To understand the molecular function of the cytoplasmic pool of this splicing protein  we performed this RNA seq experiment with the aim to identify mRNA transcripts whose expression is regulated by the cytoplasmic pool of SNRNP70. To do that  we crossed heterozygous mutant animals that are also positive for the cyt SNRNP70 transgene. We then split embryos into four groups: i sibling  ii siblings/cyt hSNRNP70  iii null  iv null/cyt hSNRNP70. Total RNA was extracted from each one of the four groups three biological replicates per sample and sequenced.,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31,,Protocols: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,16261X1,SAMEA111469061,Department of Life Sciences University of Bath,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|External Id:SAMEA111469061|INSDC center alias:Department of Life Sciences University of Bath|INSDC center name:Department of Life Sciences University of Bath|INSDC first public:2022 10 31T00:16:51Z|INSDC last update:2022 10 31T00:16:51Z|INSDC status:public|Submitter Id:E MTAB 12301:16261X1|age:28|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|disease:normal|genotype:wild type genotype|organism part:whole organism|sample name:E MTAB 12301:16261X1,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,E MTAB 12301:16261X1 p,16261X1 p,RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,Embryos were collected and raised in Danieau's solution together with 2 μΜ 4 OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP141667,Illumina NovaSeq 6000 paired end sequencing; RNA seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70,ENA FIRST PUBLIC:2022 10 31|ENA LAST UPDATE:2022 10 31|loader:fastq load.py,16261X1_190815_A00421_0101_BHFL23DRXX_S12_L001_R1_001.fastq.gz 16261X1_190815_A00421_0101_BHFL23DRXX_S12_L001_R2_001.fastq.gz,fastq fastq,2992675512.0,29339956.0,E MTAB 12301:16261X1 190815 A00421 0101 BHFL23DRXX S12 L001 R,0:51 1:51,A:768607745;C:718646844;G:713246748;T:791888447;N:285728,51,51,,,768607745,718646844,713246748,791888447,285728,ERX9900551,ERS13563097,ERA18523376,Department of Life Sciences University of Bath|European Nucleotide Archive,Department of Life Sciences University of Bath|European Nucleotide Archive,2,0.94414,0.94881,0.16725,0.16648,0.68909,0.68923,0.47873,0.48244,51,51,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2022-10-31,Pharyngula,Embryo,Whole Organism,All anatomical structures
15394,ERR12724517,ERX12099016,ERS18400121,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 WT 2,E MTAB 13886:Foxg1 WT 2,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a WT|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 WT 2 p,Foxg1 WT 2 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X14_200629_A00421_0211_BHN23CDRXX_S26_L001_R1_001.fastq.gz 18067X14_200629_A00421_0211_BHN23CDRXX_S26_L001_R2_001.fastq.gz,fastq fastq,4054926360.0,39754180.0,E MTAB 13886:18067X14 200629 A00421 0211 BHN23CDRXX S26 L001 R,0:51 1:51,A:1027112898;C:978274968;G:992295226;T:1057002684;N:240584,51,51,,,1027112898,978274968,992295226,1057002684,240584,ERX12099016,ERS18400121,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15395,ERR12724511,ERX12099010,ERS18400115,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 het 9,E MTAB 13886:Foxg1 het 9,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a het|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 het 9 p,Foxg1 het 9 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X11_200629_A00421_0211_BHN23CDRXX_S18_L001_R1_001.fastq.gz 18067X11_200629_A00421_0211_BHN23CDRXX_S18_L001_R2_001.fastq.gz,fastq fastq,2866917570.0,28107035.0,E MTAB 13886:18067X11 200629 A00421 0211 BHN23CDRXX S18 L001 R,0:51 1:51,A:725353172;C:688761995;G:699694713;T:752936948;N:170742,51,51,,,725353172,688761995,699694713,752936948,170742,ERX12099010,ERS18400115,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15396,ERR12724513,ERX12099012,ERS18400117,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 null 2,E MTAB 13886:Foxg1 null 2,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a hom|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 null 2 p,Foxg1 null 2 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X2_200629_A00421_0211_BHN23CDRXX_S27_L001_R1_001.fastq.gz 18067X2_200629_A00421_0211_BHN23CDRXX_S27_L001_R2_001.fastq.gz,fastq fastq,2908778880.0,28517440.0,E MTAB 13886:18067X2 200629 A00421 0211 BHN23CDRXX S27 L001 R,0:51 1:51,A:734785178;C:700849624;G:712519278;T:760452796;N:172004,51,51,,,734785178,700849624,712519278,760452796,172004,ERX12099012,ERS18400117,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15397,ERR12724507,ERX12099006,ERS18400111,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 het 5,E MTAB 13886:Foxg1 het 5,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a het|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 het 5 p,Foxg1 het 5 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X7_200629_A00421_0211_BHN23CDRXX_S19_L001_R1_001.fastq.gz 18067X7_200629_A00421_0211_BHN23CDRXX_S19_L001_R2_001.fastq.gz,fastq fastq,3111366282.0,30503591.0,E MTAB 13886:18067X7 200629 A00421 0211 BHN23CDRXX S19 L001 R,0:51 1:51,A:780094412;C:754603273;G:768441715;T:808041650;N:185232,51,51,,,780094412,754603273,768441715,808041650,185232,ERX12099006,ERS18400111,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15398,ERR12724519,ERX12099018,ERS18400123,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 WT 6,E MTAB 13886:Foxg1 WT 6,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a WT|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 WT 6 p,Foxg1 WT 6 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X16_200629_A00421_0211_BHN23CDRXX_S22_L001_R1_001.fastq.gz 18067X16_200629_A00421_0211_BHN23CDRXX_S22_L001_R2_001.fastq.gz,fastq fastq,3030554130.0,29711315.0,E MTAB 13886:18067X16 200629 A00421 0211 BHN23CDRXX S22 L001 R,0:51 1:51,A:770513445;C:725582256;G:737052763;T:797224111;N:181555,51,51,,,770513445,725582256,737052763,797224111,181555,ERX12099018,ERS18400123,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15399,ERR12724510,ERX12099009,ERS18400114,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 het 8,E MTAB 13886:Foxg1 het 8,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a het|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 het 8 p,Foxg1 het 8 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X10_200629_A00421_0211_BHN23CDRXX_S13_L001_R1_001.fastq.gz 18067X10_200629_A00421_0211_BHN23CDRXX_S13_L001_R2_001.fastq.gz,fastq fastq,3601017894.0,35304097.0,E MTAB 13886:18067X10 200629 A00421 0211 BHN23CDRXX S13 L001 R,0:51 1:51,A:903986531;C:871550198;G:885398218;T:939870481;N:212466,51,51,,,903986531,871550198,885398218,939870481,212466,ERX12099009,ERS18400114,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15400,ERR12724516,ERX12099015,ERS18400120,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 WT 1,E MTAB 13886:Foxg1 WT 1,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a WT|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 WT 1 p,Foxg1 WT 1 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X13_200629_A00421_0211_BHN23CDRXX_S14_L001_R1_001.fastq.gz 18067X13_200629_A00421_0211_BHN23CDRXX_S14_L001_R2_001.fastq.gz,fastq fastq,4252867662.0,41694781.0,E MTAB 13886:18067X13 200629 A00421 0211 BHN23CDRXX S14 L001 R,0:51 1:51,A:1069523245;C:1031533375;G:1047047746;T:1104513602;N:249694,51,51,,,1069523245,1031533375,1047047746,1104513602,249694,ERX12099015,ERS18400120,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15401,ERR12724505,ERX12099004,ERS18400109,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 het 2,E MTAB 13886:Foxg1 het 2,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a het|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 het 2 p,Foxg1 het 2 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X5_200629_A00421_0211_BHN23CDRXX_S21_L001_R1_001.fastq.gz 18067X5_200629_A00421_0211_BHN23CDRXX_S21_L001_R2_001.fastq.gz,fastq fastq,3490870236.0,34224218.0,E MTAB 13886:18067X5 200629 A00421 0211 BHN23CDRXX S21 L001 R,0:51 1:51,A:868722737;C:853509703;G:868564192;T:899868487;N:205117,51,51,,,868722737,853509703,868564192,899868487,205117,ERX12099004,ERS18400109,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15402,ERR12724509,ERX12099008,ERS18400113,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 het 7,E MTAB 13886:Foxg1 het 7,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a het|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 het 7 p,Foxg1 het 7 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X9_200629_A00421_0211_BHN23CDRXX_S15_L001_R1_001.fastq.gz 18067X9_200629_A00421_0211_BHN23CDRXX_S15_L001_R2_001.fastq.gz,fastq fastq,3288698484.0,32242142.0,E MTAB 13886:18067X9 200629 A00421 0211 BHN23CDRXX S15 L001 R,0:51 1:51,A:849623075;C:775307492;G:786016403;T:877557952;N:193562,51,51,,,849623075,775307492,786016403,877557952,193562,ERX12099008,ERS18400113,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15403,ERR12724512,ERX12099011,ERS18400116,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 null 1,E MTAB 13886:Foxg1 null 1,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a hom|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 null 1 p,Foxg1 null 1 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X1_200629_A00421_0211_BHN23CDRXX_S28_L001_R1_001.fastq.gz 18067X1_200629_A00421_0211_BHN23CDRXX_S28_L001_R2_001.fastq.gz,fastq fastq,3735665544.0,36624172.0,E MTAB 13886:18067X1 200629 A00421 0211 BHN23CDRXX S28 L001 R,0:51 1:51,A:930463361;C:914183345;G:929494372;T:961303244;N:221222,51,51,,,930463361,914183345,929494372,961303244,221222,ERX12099011,ERS18400116,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15404,ERR12724514,ERX12099013,ERS18400118,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 null 3,E MTAB 13886:Foxg1 null 3,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a hom|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 null 3 p,Foxg1 null 3 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X3_200629_A00421_0211_BHN23CDRXX_S25_L001_R1_001.fastq.gz 18067X3_200629_A00421_0211_BHN23CDRXX_S25_L001_R2_001.fastq.gz,fastq fastq,3581623512.0,35113956.0,E MTAB 13886:18067X3 200629 A00421 0211 BHN23CDRXX S25 L001 R,0:51 1:51,A:901782474;C:866330420;G:882309046;T:930988392;N:213180,51,51,,,901782474,866330420,882309046,930988392,213180,ERX12099013,ERS18400118,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15405,ERR12724504,ERX12099003,ERS18400108,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 het 13,E MTAB 13886:Foxg1 het 13,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a het|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 het 13 p,Foxg1 het 13 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X12_200629_A00421_0211_BHN23CDRXX_S16_L001_R1_001.fastq.gz 18067X12_200629_A00421_0211_BHN23CDRXX_S16_L001_R2_001.fastq.gz,fastq fastq,3198417570.0,31357035.0,E MTAB 13886:18067X12 200629 A00421 0211 BHN23CDRXX S16 L001 R,0:51 1:51,A:806440222;C:774073728;G:785492238;T:832222984;N:188398,51,51,,,806440222,774073728,785492238,832222984,188398,ERX12099003,ERS18400108,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15406,ERR12724508,ERX12099007,ERS18400112,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 het 6,E MTAB 13886:Foxg1 het 6,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a het|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 het 6 p,Foxg1 het 6 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X8_200629_A00421_0211_BHN23CDRXX_S17_L001_R1_001.fastq.gz 18067X8_200629_A00421_0211_BHN23CDRXX_S17_L001_R2_001.fastq.gz,fastq fastq,2829840366.0,27743533.0,E MTAB 13886:18067X8 200629 A00421 0211 BHN23CDRXX S17 L001 R,0:51 1:51,A:712954704;C:683604864;G:696251651;T:736863084;N:166063,51,51,,,712954704,683604864,696251651,736863084,166063,ERX12099007,ERS18400112,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15407,ERR12724506,ERX12099005,ERS18400110,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 het 3,E MTAB 13886:Foxg1 het 3,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a het|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 het 3 p,Foxg1 het 3 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X6_200629_A00421_0211_BHN23CDRXX_S20_L001_R1_001.fastq.gz 18067X6_200629_A00421_0211_BHN23CDRXX_S20_L001_R2_001.fastq.gz,fastq fastq,2947992168.0,28901884.0,E MTAB 13886:18067X6 200629 A00421 0211 BHN23CDRXX S20 L001 R,0:51 1:51,A:745555818;C:709342231;G:720902928;T:772016275;N:174916,51,51,,,745555818,709342231,720902928,772016275,174916,ERX12099005,ERS18400110,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15408,ERR12724515,ERX12099014,ERS18400119,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 null 6,E MTAB 13886:Foxg1 null 6,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a hom|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 null 6 p,Foxg1 null 6 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X4_200629_A00421_0211_BHN23CDRXX_S23_L001_R1_001.fastq.gz 18067X4_200629_A00421_0211_BHN23CDRXX_S23_L001_R2_001.fastq.gz,fastq fastq,3166535838.0,31044469.0,E MTAB 13886:18067X4 200629 A00421 0211 BHN23CDRXX S23 L001 R,0:51 1:51,A:781063082;C:780685132;G:798403519;T:806197923;N:186182,51,51,,,781063082,780685132,798403519,806197923,186182,ERX12099014,ERS18400119,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures
15409,ERR12724518,ERX12099017,ERS18400122,ERP158370,PRJEB73599,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E-MTAB-13886,Transcriptome Analysis,Bulk tissue RNA sequencing of individual 24hpf zebrafish larvae to compare the gene expression values between wild type and foxg1a nonsense mutants heterozygous and homozygous mutants.   The mutation is a 5bp deletion 32bp from canonical start codon; AAATG deleted.,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,,Protocols: Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,Foxg1 WT 5,E MTAB 13886:Foxg1 WT 5,,isolate:not applicable|organism:Danio rerio|organism:Danio rerio|collection date:not collected|scientific name:Danio rerio|common name:zebrafish|organism part:whole organism|developmental stage:pharyngula prim 5|genotype:Foxg1a WT|geographic location country and/or sea:not collected,,,,,,,,,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,E MTAB 13886:Foxg1 WT 5 p,Foxg1 WT 5 p,RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,Heterozygous mutant 5bp deletion 32 bp from canonical start codon; AAATG foxg1a adult zebrafish in a dlx5 6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous no interneurons in telencephalon and heterozygous reduced number of interneurons in telencephalon. Genotyping was confirmed with RNA sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit Qiagen  74004. RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo Zero Gold.,,RNA-Seq,TRANSCRIPTOMIC,Inverse rRNA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP158370,Illumina NovaSeq 6000 paired end sequencing; RNA seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings,ENA FIRST PUBLIC:2024 03 29|ENA LAST UPDATE:2024 03 29,18067X15_200629_A00421_0211_BHN23CDRXX_S24_L001_R1_001.fastq.gz 18067X15_200629_A00421_0211_BHN23CDRXX_S24_L001_R2_001.fastq.gz,fastq fastq,3253306932.0,31895166.0,E MTAB 13886:18067X15 200629 A00421 0211 BHN23CDRXX S24 L001 R,0:51 1:51,A:822636212;C:784647591;G:797221431;T:848609081;N:192617,51,51,,,822636212,784647591,797221431,848609081,192617,ERX12099017,ERS18400122,ERA29264914,European Bioinformatics Institute|European Nucleotide Archive,European Bioinformatics Institute|European Nucleotide Archive,,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,unknown,rrna_depletion,trueseq,bulk,bulk,bulk,,United Kingdom,2024-03-29,Pharyngula,Embryo,Whole Organism,All anatomical structures