rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse 101,DRR189403,DRX179868,DRS200418,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 3,SAMD00182246,,sample name:CSUS Tel 30 2w memory 3|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182246,DRX179868,CSUS Tel 30 2w memory 3,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182246,,,,2791119960.0,77531110.0,DRR189403,0:36,A:685050951;C:641519684;G:664655385;T:799677669;N:216271,36,,,,685050951,641519684,664655385,799677669,216271,DRX179868,DRS200418,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89653,,0.1773,,0.70725,,0.49873,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System 102,DRR189402,DRX179867,DRS200417,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 2,SAMD00182245,,sample name:CSUS Tel 30 2w memory 2|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182245,DRX179867,CSUS Tel 30 2w memory 2,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182245,,,,1505449224.0,41818034.0,DRR189402,0:36,A:369895057;C:346914787;G:358624651;T:429897489;N:117240,36,,,,369895057,346914787,358624651,429897489,117240,DRX179867,DRS200417,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89514,,0.17677,,0.7025,,0.49571,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System 103,DRR189401,DRX179866,DRS200416,DRP003977,PRJDB4470,Gene expression analysis of the zebrafish brain,DRP003977,Other,Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.,,,,Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 1,SAMD00182244,,sample name:CSUS Tel 30 2w memory 1|genotype:wild type|tissue:brain,,,,,,,,,Illumina HiSeq 3000 sequencing of SAMD00182244,DRX179866,CSUS Tel 30 2w memory 1,1,SureSelect Strand Specific RNA Library Prep Kit,,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,Illumina HiSeq 3000,360Application ReadForward1,DRP003977,Illumina HiSeq 3000 sequencing of SAMD00182244,,,,2088507024.0,58014084.0,DRR189401,0:36,A:516255405;C:478036869;G:496415722;T:597637091;N:161937,36,,,,516255405,478036869,496415722,597637091,161937,DRX179866,DRS200416,DRA008865,NIG|National Institute of Genetics (Japan),National Institute of Genetics (Japan),1,0.89533,,0.18553,,0.70981,,0.49554,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,poly_a,unknown,bulk,unknown,unknown,,Japan,2021-08-08,Larval,Larval,Brain,Nervous System 156,DRR067143,DRX061087,DRS034141,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Bergmann glial cells using Tg line SAGFFLF251A sample 2,SAMD00057666,,sample name:Zebrafish 251A 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:bergmann glial cells|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057666,DRX061087,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057666,,,,2040373922.0,10100861.0,DRR067143,0:101 1:101,A:577691972;C:445051245;G:472607289;T:544962357;N:61059,101,101,,,577691972,445051245,472607289,544962357,61059,DRX061087,DRS034141,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.83407,0.83802,0.12612,0.12666,0.73675,0.74079,0.4948,0.49282,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 157,DRR067142,DRX061086,DRS034140,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Bergmann glial cells using Tg line SAGFFLF251A sample 1,SAMD00057665,,sample name:Zebrafish 251A 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:bergmann glial cells|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057665,DRX061086,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057665,,,,2033951534.0,10069067.0,DRR067142,0:101 1:101,A:589558269;C:431084184;G:460501802;T:552746498;N:60781,101,101,,,589558269,431084184,460501802,552746498,60781,DRX061086,DRS034140,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.8958,0.90339,0.13764,0.1385,0.72563,0.72865,0.49373,0.49916,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 158,DRR067141,DRX061085,DRS034139,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Purkinje cells using Tg line aldoca:GAP Venus sample 3,SAMD00057664,,sample name:Zebrafish aldoca 03|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:purkinje cells|dev stage:14 dpf|replicate:biological replicate 3,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057664,DRX061085,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057664,,,,1931908608.0,9563904.0,DRR067141,0:101 1:101,A:558444394;C:409856521;G:429168864;T:534380258;N:58571,101,101,,,558444394,409856521,429168864,534380258,58571,DRX061085,DRS034139,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.88632,0.89109,0.15695,0.15773,0.75694,0.7599,0.46875,0.49236,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 159,DRR067140,DRX061084,DRS034138,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Purkinje cells using Tg line aldoca:GAP Venus sample 2,SAMD00057663,,sample name:Zebrafish aldoca 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:purkinje cells|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057663,DRX061084,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057663,,,,1851108406.0,9163903.0,DRR067140,0:101 1:101,A:537339104;C:390802782;G:416335529;T:506575762;N:55229,101,101,,,537339104,390802782,416335529,506575762,55229,DRX061084,DRS034138,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.9055,0.91341,0.1515,0.15333,0.76495,0.76719,0.49219,0.49012,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 160,DRR067139,DRX061083,DRS034137,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Purkinje cells using Tg line aldoca:GAP Venus sample 1,SAMD00057662,,sample name:Zebrafish aldoca 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:purkinje cells|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057662,DRX061083,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057662,,,,2000078962.0,9901381.0,DRR067139,0:101 1:101,A:583169141;C:420603631;G:446949308;T:549297272;N:59610,101,101,,,583169141,420603631,446949308,549297272,59610,DRX061083,DRS034137,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.9056,0.90932,0.15092,0.15179,0.76173,0.7654,0.49028,0.49604,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 161,DRR067138,DRX061082,DRS034136,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for granule cells using Tg line gSA2AzGFF152B sample 2,SAMD00057661,,sample name:Zebrafish 152B 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:granule cells|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057661,DRX061082,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057661,,,,1997432156.0,9888278.0,DRR067138,0:101 1:101,A:578466824;C:423038930;G:449779231;T:546086391;N:60780,101,101,,,578466824,423038930,449779231,546086391,60780,DRX061082,DRS034136,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.90322,0.91109,0.1463,0.14811,0.75452,0.75633,0.48303,0.48557,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 162,DRR067137,DRX061081,DRS034135,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for granule cells using Tg line gSA2AzGFF152B sample 1,SAMD00057660,,sample name:Zebrafish 152B 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:granule cells|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057660,DRX061081,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057660,,,,1908437218.0,9447709.0,DRR067137,0:101 1:101,A:552150223;C:401993096;G:427236477;T:527000978;N:56444,101,101,,,552150223,401993096,427236477,527000978,56444,DRX061081,DRS034135,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.90474,0.91043,0.17336,0.17401,0.76108,0.76359,0.483,0.48537,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 163,DRR067136,DRX061080,DRS034134,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Inferior olive nuclei using Tg line hspGFFDMC28C sample 3,SAMD00057659,,sample name:Zebrafish 28C 03|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:hindbrain|cell type:inferior olive nuclei|dev stage:14 dpf|replicate:biological replicate 3,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057659,DRX061080,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057659,,,,1982971178.0,9816689.0,DRR067136,0:101 1:101,A:504064758;C:488246993;G:520130333;T:470469799;N:59295,101,101,,,504064758,488246993,520130333,470469799,59295,DRX061080,DRS034134,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.53386,0.54375,0.06072,0.06189,0.76351,0.76641,0.4872,0.48857,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 164,DRR067135,DRX061079,DRS034133,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Inferior olive nuclei using Tg line hspGFFDMC28C sample 2,SAMD00057658,,sample name:Zebrafish 28C 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:hindbrain|cell type:inferior olive nuclei|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057658,DRX061079,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057658,,,,1961048320.0,9708160.0,DRR067135,0:101 1:101,A:557469151;C:424691789;G:455244994;T:523583601;N:58785,101,101,,,557469151,424691789,455244994,523583601,58785,DRX061079,DRS034133,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.84854,0.8526,0.10311,0.1042,0.75227,0.75499,0.49321,0.49435,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 165,DRR067134,DRX061078,DRS034132,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for Inferior olive nuclei using Tg line hspGFFDMC28C sample 1,SAMD00057657,,sample name:Zebrafish 28C 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:hindbrain|cell type:inferior olive nuclei|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057657,DRX061078,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057657,,,,1931802760.0,9563380.0,DRR067134,0:101 1:101,A:546501397;C:422500710;G:452200521;T:510543489;N:56643,101,101,,,546501397,422500710,452200521,510543489,56643,DRX061078,DRS034132,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.85439,0.85613,0.09867,0.09897,0.76871,0.77082,0.48943,0.48936,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 166,DRR067133,DRX061077,DRS034131,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for eurydendroid cells using Tg line hspzGFFgDMC156A sample 2,SAMD00057656,,sample name:Zebrafish 156A 02|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:eurydendroid cells|dev stage:14 dpf|replicate:biological replicate 2,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057656,DRX061077,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057656,,,,2054289096.0,10169748.0,DRR067133,0:101 1:101,A:563764176;C:464768863;G:497506420;T:528187503;N:62134,101,101,,,563764176,464768863,497506420,528187503,62134,DRX061077,DRS034131,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.69311,0.6968,0.12309,0.12389,0.76428,0.76676,0.48379,0.48227,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 167,DRR067132,DRX061076,DRS034130,DRP003275,PRJDB4941,Gene expression profiling of granule cells and Purkinje cells in zebrafish cerebellum,DRP003275,Other,An RNA seq analysis was performed using zebrafish granule cells Purkinje cells IO neurons and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired end libraries employing the Quartz seq method for low amount total RNA.,,,,Zebrafish RNA seq for eurydendroid cells using Tg line hspzGFFgDMC156A sample 1,SAMD00057655,,sample name:Zebrafish 156A 01|strain:Tg|biomaterial provider:Bioscience and Biotechnology Center Nagoya University|tissue type:cerebellum|cell type:eurydendroid cells|dev stage:14 dpf|replicate:biological replicate 1,,,,,,,,,Illumina HiSeq 1500 paired end sequencing of SAMD00057655,DRX061076,1,1,Quartz seq for low amount total RNA,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 1500,2020Application ReadForward11Application ReadReverse102,DRP003275,Illumina HiSeq 1500 paired end sequencing of SAMD00057655,,,,2152665722.0,10656761.0,DRR067132,0:101 1:101,A:585252781;C:495382257;G:522677749;T:549287359;N:65576,101,101,,,585252781,495382257,522677749,549287359,65576,DRX061076,DRS034130,DRA004955,RIKEN_CLST_DBFDI|Phyloinformatics Unit,RIKEN CLST,2,0.67771,0.68253,0.12949,0.1303,0.77216,0.77542,0.4951,0.49452,101,101,B,B,biological fallback assumption,illumina,hiseq_era,unknown,cdna_unspecified,unknown,sc,single_cell_plate,quartzseq,,Japan,2016-09-19,Larval,Larval,Brain,Nervous System 9331,ERR2862354,ERX2868592,ERS2866329,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,TUC,SAMEA5055152,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055152|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:TUC|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:TUC|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:TUC p,TUC p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,AGCGATAG_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz AGCGATAG_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz,fastq fastq,12890102400.0,64450512.0,E MTAB 7349:AGCGATAG AGGCTATA 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3347844573;C:3078635366;G:3118813009;T:3343385073;N:1424379,100,100,,,3347844573,3078635366,3118813009,3343385073,1424379,ERX2868592,ERS2866329,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.89165,0.8896,0.26768,0.26518,0.75142,0.75459,0.60855,0.6105,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System 9332,ERR2862353,ERX2868591,ERS2866328,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,TUB,SAMEA5055151,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055151|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:TUB|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:TUB|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:TUB p,TUB p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,TCTCGCGC_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz TCTCGCGC_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz,fastq fastq,15345882200.0,76729411.0,E MTAB 7349:TCTCGCGC AGGCTATA 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:4257362303;C:3394893224;G:3451042837;T:4240872487;N:1711349,100,100,,,4257362303,3394893224,3451042837,4240872487,1711349,ERX2868591,ERS2866328,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.86888,0.86735,0.45025,0.44476,0.72036,0.72529,0.56048,0.56274,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System 9333,ERR2862352,ERX2868590,ERS2866327,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,TUA,SAMEA5055150,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055150|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:TUA|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:TUA|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:TUA p,TUA p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,TCCGCGAA_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz TCCGCGAA_AGGCTATA__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz,fastq fastq,11985547000.0,59927735.0,E MTAB 7349:TCCGCGAA AGGCTATA 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3239652711;C:2732996991;G:2737809176;T:3273773368;N:1314754,100,100,,,3239652711,2732996991,2737809176,3273773368,1314754,ERX2868590,ERS2866327,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.91524,0.91585,0.32418,0.32053,0.72616,0.72671,0.59362,0.42478,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System 9334,ERR2862351,ERX2868589,ERS2866326,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,HRASC,SAMEA5055149,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055149|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:HRASC|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:HRASC|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:HRASC p,HRASC p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,CGGCTATG_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz CGGCTATG_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz,fastq fastq,11297151800.0,56485759.0,E MTAB 7349:CGGCTATG GTCAGTAC 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3202944476;C:2429970659;G:2461948398;T:3201079699;N:1208568,100,100,,,3202944476,2429970659,2461948398,3201079699,1208568,ERX2868589,ERS2866326,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.89842,0.86269,0.32455,0.30708,0.74245,0.75191,0.7454,0.74185,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System 9335,ERR2862350,ERX2868588,ERS2866325,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,HRASB 1A,SAMEA5055148,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055148|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:HRASB 1A|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:HRASB 1A|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:HRASB 1A p,HRASB 1A p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,TCTCGCGC_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz TCTCGCGC_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz,fastq fastq,13035635000.0,65178175.0,E MTAB 7349:TCTCGCGC GTCAGTAC 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3518767071;C:2991842048;G:3005014068;T:3518541297;N:1470516,100,100,,,3518767071,2991842048,3005014068,3518541297,1470516,ERX2868588,ERS2866325,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.89434,0.89384,0.28234,0.28093,0.7274,0.72813,0.6198,0.62424,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System 9336,ERR2862349,ERX2868587,ERS2866324,ERP111743,PRJEB29441,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E-MTAB-7349,Transcriptome Analysis,Oncogenic transformation of individual cell fates by developmental signaling cascades and transcription factors triggers diverse cancer types. Chordoma is a rare aggressive tumor arising from transformed notochord remnants. Various potentially oncogenic factors have been found deregulated in chordoma and its metastases yet clear causation remains uncertain. In particular expression of the notochord controlling transcription factor Brachyury is hypothesized as key molecular driver in chordoma formation yet an in vivo model to causally test its oncogenic potential in the notochord is missing. Here we apply a zebrafish model of chordoma onset to identify the notochord transforming potential of tumor implicated candidate genes in vivo. We find that overexpression of human and zebrafish Brachyury including a version with augmented transcriptional activity is insufficient to initiate notochord hyperplasia in vivo. In contrast the repeatedly chordoma implicated receptor tyrosine kinase RTK genes EGFR and KDR/VEGFR2 are sufficient to transform developmental notochord cells akin to direct activation of Ras. Analysis of transcriptome and sub cellular organization from transformed notochords suggests that aberrant activation of RTK/Ras signaling attenuates processes required for the differentiation of notochord cells. Taken together our results provide first in vivo indication for a lack of tumor initiating potential of Brachyury expression in the notochord and suggest activated RTK signaling as potent hyperplasia initiating event in chordoma.,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 10 30,,Protocols: 8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,2A,SAMEA5055147,UZH,ENA FIRST PUBLIC:2018 12 01T17:03:21Z|ENA LAST UPDATE:2018 10 30T13:30:13Z|External Id:SAMEA5055147|INSDC center name:UZH|INSDC first public:2018 12 01T17:03:21Z|INSDC last update:2018 10 30T13:30:13Z|INSDC status:public|Submitter Id:E MTAB 7349:2A|age:8|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12|individual:mixed pool of 30 to 50 embryos|organism part:notochord|sample name:E MTAB 7349:2A|scientific name:Danio rerio|sex:mixed,,,,,,,,,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,E MTAB 7349:2A p,2A p,Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,8 dpf wildtype and Tgtwhh:Gal4;TgUAS:EGFP HRASV12 were euthanized with 3% Tricaine methanesulfonate Sigma. Embryos were decapitated and incubated in Tripsin EDTA Sigma for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol LS Ambion. We isolated 30 50 notochords per replicate with a total of 3 replicates per condition 3x wildtype 3x HRASV12. Notochord RNA was extracted following the manufacturer's protocol using Trizol LS. RNA seq libraries were constructed using the Truseq stranded total RNA kit.,Experimental Factor: genotype:Tgtwhh:Gal4;TgUAS:EGFP HRASV12,RNA-Seq,TRANSCRIPTOMIC,RANDOM,PAIRED,ILLUMINA,Illumina HiSeq 2500,2000FApplication ReadForward11RApplication ReadReverse101,ERP111743,Illumina HiSeq 2500 paired end sequencing; Receptor Tyrosine Kinase pathway activation is sufficient to trigger chordoma in zebrafish,ENA FIRST PUBLIC:2018 12 01|ENA LAST UPDATE:2018 11 16,TCCGCGAA_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_2.fq.gz TCCGCGAA_GTCAGTAC__160520_I127_FCH732GBBXX_L8_CDKPEI160513002_1.fq.gz,fastq fastq,11671772800.0,58358864.0,E MTAB 7349:TCCGCGAA GTCAGTAC 160520 I127 FCH732GBBXX L8 CDKPEI160513002 ,0:100 1:100,A:3164806186;C:2661342106;G:2677258036;T:3167072669;N:1293803,100,100,,,3164806186,2661342106,2677258036,3167072669,1293803,ERX2868587,ERS2866324,ERA1640550,European Nucleotide Archive,European Nucleotide Archive,2,0.89879,0.89878,0.28162,0.28061,0.73212,0.73452,0.59632,0.60115,100,100,B,B,biological fallback assumption,illumina,hiseq_era,unknown,random_priming,trueseq,bulk,unknown,unknown,,Unknown,2018-10-30,Larval,Larval,Brain,Nervous System 10176,ERR5858457,ERX5504346,ERS6343450,ERP128749,PRJEB44676,scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,E-MTAB-10390,Transcriptome Analysis,To analyse lesion induced gene regulation in progenitor cells at single cell resolution we performed single cell RNAseq on FACS isolated her4.3:GFP progenitor cells from the spinal cord at 24 hours post lesion hpl post spinal injury at 3 dpf dpf compared to age matched uninjured animals.,ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24,,Protocols: Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.,Naive,SAMEA8658904,University Of Edinburgh,ENA first public:2021 05 24|ENA last update:2021 05 24|External Id:SAMEA8658904|INSDC center alias:UOE|INSDC center name:University Of Edinburgh|INSDC first public:2021 05 24T00:14:32Z|INSDC last update:2021 05 24T00:14:32Z|INSDC status:public|Submitter Id:E MTAB 10390:Naive|age:4|broker name:ArrayExpress|cell type:ependymo radial glial cell|common name:zebrafish|developmental stage:larval day 4|immunophenotype:Her4.3+ positive|organism part:spinal cord|sample name:E MTAB 10390:Naive|sex:mixed|strain:WIK,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesi1d and unlesi1d zebrafish larvae spinal cord,E MTAB 10390:Naive p,Naive p,scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.,Experimental Factor: injury:n1,RNA-Seq,TRANSCRIPTOMIC SINGLE CELL,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP128749,Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24,Naive.bam,bam,44972162730.0,499690697.0,E MTAB 10390:Naive,0:90,A:13658826375;C:8733304343;G:9348137569;T:13228064343;N:3830100,90,,,,13658826375,8733304343,9348137569,13228064343,3830100,ERX5504346,ERS6343450,ERA4142789,University Of Edinburgh|European Nucleotide Archive,University Of Edinburgh|European Nucleotide Archive,1,0.89428,,0.32693,,0.75276,,0.5314,,90,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,sc,single_cell_droplet,10x,,United Kingdom,2021-05-24,Larval,Larval,Spinal Cord,Nervous System 10177,ERR5858456,ERX5504345,ERS6343449,ERP128749,PRJEB44676,scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,E-MTAB-10390,Transcriptome Analysis,To analyse lesion induced gene regulation in progenitor cells at single cell resolution we performed single cell RNAseq on FACS isolated her4.3:GFP progenitor cells from the spinal cord at 24 hours post lesion hpl post spinal injury at 3 dpf dpf compared to age matched uninjured animals.,ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24,,Protocols: Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.,Lesi1d,SAMEA8658903,University Of Edinburgh,ENA first public:2021 05 24|ENA last update:2021 05 24|External Id:SAMEA8658903|INSDC center alias:UOE|INSDC center name:University Of Edinburgh|INSDC first public:2021 05 24T00:14:32Z|INSDC last update:2021 05 24T00:14:32Z|INSDC status:public|Submitter Id:E MTAB 10390:Lesi1d|age:4|broker name:ArrayExpress|cell type:ependymo radial glial cell|common name:zebrafish|developmental stage:larval day 4|immunophenotype:Her4.3+ positive|injury:spinal injury lesion|organism part:spinal cord|sample name:E MTAB 10390:Lesi1d|sex:mixed|strain:WIK,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesi1d and unlesi1d zebrafish larvae spinal cord,E MTAB 10390:Lesioned p,Lesioned p,scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.,Experimental Factor: injury:spinal injury lesion,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP128749,Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord,ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24,Lesioned.bam,bam,49902588630.0,554473207.0,E MTAB 10390:Lesioned,0:90,A:14713351178;C:10191139050;G:10897887675;T:14095967201;N:4243526,90,,,,14713351178,10191139050,10897887675,14095967201,4243526,ERX5504345,ERS6343449,ERA4142789,University Of Edinburgh|European Nucleotide Archive,University Of Edinburgh|European Nucleotide Archive,1,0.91197,,0.29137,,0.7568,,0.56523,,90,,B,,usable mapping rate,illumina,novaseq_era,unknown,poly_a,unknown,sc,single_cell_droplet,10x,,United Kingdom,2021-05-24,Larval,Larval,Spinal Cord,Nervous System 10407,ERR9077202,ERX8620336,ERS10857224,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT5S8,SAMEA13254181,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT5S8 s,Sv2aWT5S8 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT5S8L001-1.fastq.gz,fastq,652046291.0,8633105.0,E MTAB 11505:sv2aWT5S8L001,0:75.53 1:0,A:159570243;C:156420495;G:154853315;T:181073030;N:129208,75,0,,,159570243,156420495,154853315,181073030,129208,ERX8620336,ERS10857224,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95245,,0.1148,,0.65756,,0.47011,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10408,ERR9077203,ERX8620336,ERS10857224,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT5S8,SAMEA13254181,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT5S8 s,Sv2aWT5S8 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT5S8L002-1.fastq.gz,fastq,653840525.0,8656886.0,E MTAB 11505:sv2aWT5S8L002,0:75.53 1:0,A:159902458;C:156826842;G:155509070;T:181475104;N:127051,75,0,,,159902458,156826842,155509070,181475104,127051,ERX8620336,ERS10857224,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95226,,0.1156,,0.65792,,0.4667,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10409,ERR9077204,ERX8620336,ERS10857224,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT5S8,SAMEA13254181,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT5S8 s,Sv2aWT5S8 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT5S8L003-1.fastq.gz,fastq,659584196.0,8732885.0,E MTAB 11505:sv2aWT5S8L003,0:75.53 1:0,A:161257146;C:158299734;G:156793443;T:183128972;N:104901,75,0,,,161257146,158299734,156793443,183128972,104901,ERX8620336,ERS10857224,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95298,,0.11485,,0.65798,,0.46853,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10410,ERR9077205,ERX8620336,ERS10857224,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT5S8,SAMEA13254181,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT5S8 s,Sv2aWT5S8 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT5S8L004-1.fastq.gz,fastq,666627746.0,8826123.0,E MTAB 11505:sv2aWT5S8L004,0:75.53 1:0,A:162975131;C:159937258;G:158506242;T:185106149;N:102966,75,0,,,162975131,159937258,158506242,185106149,102966,ERX8620336,ERS10857224,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.9531,,0.11425,,0.65736,,0.47309,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10411,ERR9077198,ERX8620335,ERS10857223,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT4S7,SAMEA13254180,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT4S7 s,Sv2aWT4S7 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT4S7L001-1.fastq.gz,fastq,683751258.0,9051795.0,E MTAB 11505:sv2aWT4S7L001,0:75.54 1:0,A:162889923;C:167127644;G:164779384;T:188822907;N:131400,75,0,,,162889923,167127644,164779384,188822907,131400,ERX8620335,ERS10857223,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95739,,0.0959,,0.6606,,0.46826,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10412,ERR9077199,ERX8620335,ERS10857223,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT4S7,SAMEA13254180,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT4S7 s,Sv2aWT4S7 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT4S7L002-1.fastq.gz,fastq,684187711.0,9057591.0,E MTAB 11505:sv2aWT4S7L002,0:75.54 1:0,A:162923879;C:167246213;G:165071145;T:188818774;N:127700,75,0,,,162923879,167246213,165071145,188818774,127700,ERX8620335,ERS10857223,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95724,,0.0959,,0.66036,,0.47026,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10413,ERR9077200,ERX8620335,ERS10857223,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT4S7,SAMEA13254180,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT4S7 s,Sv2aWT4S7 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT4S7L003-1.fastq.gz,fastq,692320213.0,9165132.0,E MTAB 11505:sv2aWT4S7L003,0:75.54 1:0,A:164772792;C:169329920;G:166952052;T:191164422;N:101027,75,0,,,164772792,169329920,166952052,191164422,101027,ERX8620335,ERS10857223,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95786,,0.09701,,0.66131,,0.47564,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10414,ERR9077201,ERX8620335,ERS10857223,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT4S7,SAMEA13254180,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT4S7 s,Sv2aWT4S7 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT4S7L004-1.fastq.gz,fastq,697047881.0,9227702.0,E MTAB 11505:sv2aWT4S7L004,0:75.54 1:0,A:165881728;C:170388393;G:168105900;T:192572068;N:99792,75,0,,,165881728,170388393,168105900,192572068,99792,ERX8620335,ERS10857223,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.9584,,0.09565,,0.66115,,0.46967,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10415,ERR9077194,ERX8620334,ERS10857222,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT3S2,SAMEA13254179,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT3S2 s,Sv2aWT3S2 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT3S2L001-1.fastq.gz,fastq,723508205.0,9579193.0,E MTAB 11505:sv2aWT3S2L001,0:75.53 1:0,A:177832489;C:174193135;G:169978598;T:201360184;N:143799,75,0,,,177832489,174193135,169978598,201360184,143799,ERX8620334,ERS10857222,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95218,,0.11732,,0.65916,,0.47955,,71,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10416,ERR9077195,ERX8620334,ERS10857222,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT3S2,SAMEA13254179,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT3S2 s,Sv2aWT3S2 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT3S2L002-1.fastq.gz,fastq,726190024.0,9614674.0,E MTAB 11505:sv2aWT3S2L002,0:75.53 1:0,A:178408224;C:174776263;G:170820745;T:202047224;N:137568,75,0,,,178408224,174776263,170820745,202047224,137568,ERX8620334,ERS10857222,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95093,,0.11567,,0.65865,,0.46877,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10417,ERR9077196,ERX8620334,ERS10857222,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT3S2,SAMEA13254179,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT3S2 s,Sv2aWT3S2 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT3S2L003-1.fastq.gz,fastq,735499955.0,9737845.0,E MTAB 11505:sv2aWT3S2L003,0:75.53 1:0,A:180659962;C:177141362;G:172905323;T:204678308;N:115000,75,0,,,180659962,177141362,172905323,204678308,115000,ERX8620334,ERS10857222,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95233,,0.11614,,0.65898,,0.47114,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10418,ERR9077197,ERX8620334,ERS10857222,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT3S2,SAMEA13254179,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT3S2 s,Sv2aWT3S2 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT3S2L004-1.fastq.gz,fastq,745285512.0,9867337.0,E MTAB 11505:sv2aWT3S2L004,0:75.53 1:0,A:183067487;C:179408382;G:175250814;T:207445912;N:112917,75,0,,,183067487,179408382,175250814,207445912,112917,ERX8620334,ERS10857222,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95245,,0.11655,,0.65857,,0.47087,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10419,ERR9077190,ERX8620333,ERS10857221,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT1S1,SAMEA13254178,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT1S1 s,Sv2aWT1S1 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT1S1L001-1.fastq.gz,fastq,647414748.0,8571883.0,E MTAB 11505:sv2aWT1S1L001,0:75.53 1:0,A:154593859;C:160498057;G:153809612;T:178389112;N:124108,75,0,,,154593859,160498057,153809612,178389112,124108,ERX8620333,ERS10857221,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95955,,0.08686,,0.65955,,0.47406,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10420,ERR9077191,ERX8620333,ERS10857221,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT1S1,SAMEA13254178,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT1S1 s,Sv2aWT1S1 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT1S1L002-1.fastq.gz,fastq,646657758.0,8561861.0,E MTAB 11505:sv2aWT1S1L002,0:75.53 1:0,A:154348044;C:160294625;G:153804048;T:178092212;N:118829,75,0,,,154348044,160294625,153804048,178092212,118829,ERX8620333,ERS10857221,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95942,,0.08632,,0.65991,,0.47539,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10421,ERR9077192,ERX8620333,ERS10857221,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT1S1,SAMEA13254178,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT1S1 s,Sv2aWT1S1 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT1S1L003-1.fastq.gz,fastq,654781451.0,8669333.0,E MTAB 11505:sv2aWT1S1L003,0:75.53 1:0,A:156252744;C:162396721;G:155681450;T:180352911;N:97625,75,0,,,156252744,162396721,155681450,180352911,97625,ERX8620333,ERS10857221,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95972,,0.08498,,0.66137,,0.47457,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10422,ERR9077193,ERX8620333,ERS10857221,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aWT1S1,SAMEA13254178,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aWT1S1 s,Sv2aWT1S1 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/+,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aWT1S1L004-1.fastq.gz,fastq,659716937.0,8734618.0,E MTAB 11505:sv2aWT1S1L004,0:75.53 1:0,A:157367211;C:163585842;G:156911139;T:181757809;N:94936,75,0,,,157367211,163585842,156911139,181757809,94936,ERX8620333,ERS10857221,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95985,,0.08585,,0.65857,,0.47349,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10423,ERR9077186,ERX8620332,ERS10857220,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO6S12,SAMEA13254177,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO6S12 s,Sv2aHO6S12 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO6S12L001-1.fastq.gz,fastq,675018261.0,8937958.0,E MTAB 11505:sv2aHO6S12L001,0:75.52 1:0,A:166564263;C:160539328;G:160003980;T:187773509;N:137181,75,0,,,166564263,160539328,160003980,187773509,137181,ERX8620332,ERS10857220,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94964,,0.13009,,0.65413,,0.47079,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10424,ERR9077187,ERX8620332,ERS10857220,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO6S12,SAMEA13254177,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO6S12 s,Sv2aHO6S12 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO6S12L002-1.fastq.gz,fastq,672995667.0,8911161.0,E MTAB 11505:sv2aHO6S12L002,0:75.52 1:0,A:165990319;C:160039398;G:159722501;T:187108781;N:134668,75,0,,,165990319,160039398,159722501,187108781,134668,ERX8620332,ERS10857220,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94837,,0.13029,,0.65744,,0.47959,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10425,ERR9077188,ERX8620332,ERS10857220,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO6S12,SAMEA13254177,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO6S12 s,Sv2aHO6S12 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO6S12L003-1.fastq.gz,fastq,683276767.0,9047147.0,E MTAB 11505:sv2aHO6S12L003,0:75.52 1:0,A:168501690;C:162583852;G:162063190;T:190018001;N:110034,75,0,,,168501690,162583852,162063190,190018001,110034,ERX8620332,ERS10857220,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94983,,0.13248,,0.6565,,0.47977,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10426,ERR9077189,ERX8620332,ERS10857220,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO6S12,SAMEA13254177,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO6S12 s,Sv2aHO6S12 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO6S12L004-1.fastq.gz,fastq,686469910.0,9089431.0,E MTAB 11505:sv2aHO6S12L004,0:75.52 1:0,A:169287189;C:163276361;G:162844724;T:190954218;N:107418,75,0,,,169287189,163276361,162844724,190954218,107418,ERX8620332,ERS10857220,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95061,,0.13029,,0.65389,,0.48044,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10427,ERR9077182,ERX8620331,ERS10857219,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO4S10,SAMEA13254176,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO4S10 s,Sv2aHO4S10 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO4S10L001-1.fastq.gz,fastq,713858966.0,9452474.0,E MTAB 11505:sv2aHO4S10L001,0:75.52 1:0,A:177913695;C:169425317;G:167540012;T:198835532;N:144410,75,0,,,177913695,169425317,167540012,198835532,144410,ERX8620331,ERS10857219,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94848,,0.12981,,0.6636,,0.47772,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10428,ERR9077183,ERX8620331,ERS10857219,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO4S10,SAMEA13254176,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO4S10 s,Sv2aHO4S10 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO4S10L002-1.fastq.gz,fastq,717619593.0,9502301.0,E MTAB 11505:sv2aHO4S10L002,0:75.52 1:0,A:178830542;C:170284720;G:168594514;T:199773382;N:136435,75,0,,,178830542,170284720,168594514,199773382,136435,ERX8620331,ERS10857219,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94812,,0.12868,,0.6645,,0.47281,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10429,ERR9077184,ERX8620331,ERS10857219,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO4S10,SAMEA13254176,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO4S10 s,Sv2aHO4S10 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO4S10L003-1.fastq.gz,fastq,727263626.0,9629858.0,E MTAB 11505:sv2aHO4S10L003,0:75.52 1:0,A:181201278;C:172619520;G:170780953;T:202550397;N:111478,75,0,,,181201278,172619520,170780953,202550397,111478,ERX8620331,ERS10857219,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94768,,0.12827,,0.66596,,0.47332,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10430,ERR9077185,ERX8620331,ERS10857219,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO4S10,SAMEA13254176,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO4S10 s,Sv2aHO4S10 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO4S10L004-1.fastq.gz,fastq,736595278.0,9753337.0,E MTAB 11505:sv2aHO4S10L004,0:75.52 1:0,A:183510872;C:174806534;G:173027794;T:205140640;N:109438,75,0,,,183510872,174806534,173027794,205140640,109438,ERX8620331,ERS10857219,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94925,,0.12904,,0.66417,,0.47297,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10431,ERR9077178,ERX8620330,ERS10857218,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO3S6,SAMEA13254175,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO3S6 s,Sv2aHO3S6 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO3S6L001-1.fastq.gz,fastq,710756305.0,9410862.0,E MTAB 11505:sv2aHO3S6L001,0:75.53 1:0,A:175585613;C:170261120;G:166658917;T:198106388;N:144267,75,0,,,175585613,170261120,166658917,198106388,144267,ERX8620330,ERS10857218,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94827,,0.14002,,0.66785,,0.474,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10432,ERR9077179,ERX8620330,ERS10857218,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO3S6,SAMEA13254175,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO3S6 s,Sv2aHO3S6 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO3S6L002-1.fastq.gz,fastq,715330324.0,9471415.0,E MTAB 11505:sv2aHO3S6L002,0:75.53 1:0,A:176679775;C:171311031;G:167903237;T:199299483;N:136798,75,0,,,176679775,171311031,167903237,199299483,136798,ERX8620330,ERS10857218,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.9469,,0.13915,,0.67004,,0.47654,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10433,ERR9077180,ERX8620330,ERS10857218,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO3S6,SAMEA13254175,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO3S6 s,Sv2aHO3S6 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO3S6L003-1.fastq.gz,fastq,722810562.0,9570364.0,E MTAB 11505:sv2aHO3S6L003,0:75.53 1:0,A:178442845;C:173192786;G:169595085;T:201465522;N:114324,75,0,,,178442845,173192786,169595085,201465522,114324,ERX8620330,ERS10857218,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94918,,0.13979,,0.66902,,0.47572,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10434,ERR9077181,ERX8620330,ERS10857218,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHO3S6,SAMEA13254175,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHO3S6 s,Sv2aHO3S6 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHO3S6L004-1.fastq.gz,fastq,732990507.0,9705054.0,E MTAB 11505:sv2aHO3S6L004,0:75.53 1:0,A:180951130;C:175567778;G:172037542;T:204323488;N:110569,75,0,,,180951130,175567778,172037542,204323488,110569,ERX8620330,ERS10857218,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.94812,,0.13893,,0.67085,,0.47502,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10435,ERR9077174,ERX8620329,ERS10857217,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET7S9,SAMEA13254174,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET7S9 s,Sv2aHET7S9 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET7S9L001-1.fastq.gz,fastq,773209432.0,10238087.0,E MTAB 11505:sv2aHET7S9L001,0:75.52 1:0,A:191962953;C:184699376;G:180774008;T:215620759;N:152336,75,0,,,191962953,184699376,180774008,215620759,152336,ERX8620329,ERS10857217,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95047,,0.12485,,0.65705,,0.4775,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10436,ERR9077175,ERX8620329,ERS10857217,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET7S9,SAMEA13254174,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET7S9 s,Sv2aHET7S9 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET7S9L002-1.fastq.gz,fastq,773106152.0,10236677.0,E MTAB 11505:sv2aHET7S9L002,0:75.52 1:0,A:191812833;C:184676419;G:180997547;T:215473732;N:145621,75,0,,,191812833,184676419,180997547,215473732,145621,ERX8620329,ERS10857217,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95016,,0.12464,,0.65482,,0.47419,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10437,ERR9077176,ERX8620329,ERS10857217,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET7S9,SAMEA13254174,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET7S9 s,Sv2aHET7S9 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET7S9L003-1.fastq.gz,fastq,783061051.0,10368445.0,E MTAB 11505:sv2aHET7S9L003,0:75.52 1:0,A:194289152;C:187108364;G:183177036;T:218370500;N:115999,75,0,,,194289152,187108364,183177036,218370500,115999,ERX8620329,ERS10857217,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95172,,0.12488,,0.65445,,0.48157,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10438,ERR9077177,ERX8620329,ERS10857217,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET7S9,SAMEA13254174,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET7S9 s,Sv2aHET7S9 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET7S9L004-1.fastq.gz,fastq,790150025.0,10462214.0,E MTAB 11505:sv2aHET7S9L004,0:75.52 1:0,A:196056500;C:188746833;G:184927588;T:220305922;N:113182,75,0,,,196056500,188746833,184927588,220305922,113182,ERX8620329,ERS10857217,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95285,,0.12478,,0.65551,,0.4763,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10439,ERR9077170,ERX8620328,ERS10857216,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET4S5,SAMEA13254173,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET4S5 s,Sv2aHET4S5 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET4S5L001-1.fastq.gz,fastq,685650097.0,9076481.0,E MTAB 11505:sv2aHET4S5L001,0:75.54 1:0,A:161812875;C:167870520;G:166985310;T:188850530;N:130862,75,0,,,161812875,167870520,166985310,188850530,130862,ERX8620328,ERS10857216,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95733,,0.09974,,0.65691,,0.47392,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10440,ERR9077171,ERX8620328,ERS10857216,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET4S5,SAMEA13254173,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET4S5 s,Sv2aHET4S5 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET4S5L002-1.fastq.gz,fastq,684402241.0,9059974.0,E MTAB 11505:sv2aHET4S5L002,0:75.54 1:0,A:161493428;C:167520248;G:166882054;T:188383816;N:122695,75,0,,,161493428,167520248,166882054,188383816,122695,ERX8620328,ERS10857216,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95537,,0.09915,,0.65719,,0.48261,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10441,ERR9077172,ERX8620328,ERS10857216,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET4S5,SAMEA13254173,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET4S5 s,Sv2aHET4S5 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET4S5L003-1.fastq.gz,fastq,694099126.0,9188245.0,E MTAB 11505:sv2aHET4S5L003,0:75.54 1:0,A:163727378;C:169989210;G:169143224;T:191137588;N:101726,75,0,,,163727378,169989210,169143224,191137588,101726,ERX8620328,ERS10857216,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95629,,0.09861,,0.65705,,0.47187,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10442,ERR9077173,ERX8620328,ERS10857216,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET4S5,SAMEA13254173,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET4S5 s,Sv2aHET4S5 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET4S5L004-1.fastq.gz,fastq,698753836.0,9249820.0,E MTAB 11505:sv2aHET4S5L004,0:75.54 1:0,A:164809473;C:171057633;G:170349692;T:192441413;N:95625,75,0,,,164809473,171057633,170349692,192441413,95625,ERX8620328,ERS10857216,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95613,,0.09885,,0.65798,,0.47227,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10443,ERR9077166,ERX8620327,ERS10857215,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET3S4,SAMEA13254172,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET3S4 s,Sv2aHET3S4 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET3S4L001-1.fastq.gz,fastq,691298605.0,9151512.0,E MTAB 11505:sv2aHET3S4L001,0:75.54 1:0,A:164743919;C:170752099;G:164763621;T:190912044;N:126922,75,0,,,164743919,170752099,164763621,190912044,126922,ERX8620327,ERS10857215,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95684,,0.09604,,0.6603,,0.47285,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10444,ERR9077167,ERX8620327,ERS10857215,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET3S4,SAMEA13254172,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET3S4 s,Sv2aHET3S4 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET3S4L002-1.fastq.gz,fastq,694080975.0,9188259.0,E MTAB 11505:sv2aHET3S4L002,0:75.54 1:0,A:165336810;C:171397273;G:165632321;T:191591872;N:122699,75,0,,,165336810,171397273,165632321,191591872,122699,ERX8620327,ERS10857215,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95612,,0.09694,,0.65993,,0.46918,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10445,ERR9077168,ERX8620327,ERS10857215,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET3S4,SAMEA13254172,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET3S4 s,Sv2aHET3S4 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET3S4L003-1.fastq.gz,fastq,703689889.0,9315387.0,E MTAB 11505:sv2aHET3S4L003,0:75.54 1:0,A:167575230;C:173881977;G:167845574;T:194289403;N:97705,75,0,,,167575230,173881977,167845574,194289403,97705,ERX8620327,ERS10857215,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.9577,,0.09608,,0.66042,,0.47022,,74,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10446,ERR9077169,ERX8620327,ERS10857215,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET3S4,SAMEA13254172,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET3S4 s,Sv2aHET3S4 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET3S4L004-1.fastq.gz,fastq,712116538.0,9426961.0,E MTAB 11505:sv2aHET3S4L004,0:75.54 1:0,A:169598596;C:175885948;G:169866534;T:196669400;N:96060,75,0,,,169598596,175885948,169866534,196669400,96060,ERX8620327,ERS10857215,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95734,,0.09716,,0.66054,,0.47257,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10447,ERR9077162,ERX8620326,ERS10857214,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET2S3,SAMEA13254171,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET2S3 s,Sv2aHET2S3 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET2S3L001-1.fastq.gz,fastq,733440522.0,9709422.0,E MTAB 11505:sv2aHET2S3L001,0:75.54 1:0,A:173670000;C:181175353;G:175392793;T:203059256;N:143120,75,0,,,173670000,181175353,175392793,203059256,143120,ERX8620326,ERS10857214,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95885,,0.08758,,0.65393,,0.47249,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10448,ERR9077163,ERX8620326,ERS10857214,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET2S3,SAMEA13254171,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET2S3 s,Sv2aHET2S3 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET2S3L002-1.fastq.gz,fastq,732121929.0,9691967.0,E MTAB 11505:sv2aHET2S3L002,0:75.54 1:0,A:173332091;C:180805738;G:175271736;T:202574727;N:137637,75,0,,,173332091,180805738,175271736,202574727,137637,ERX8620326,ERS10857214,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95775,,0.08879,,0.6564,,0.4718,,76,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10449,ERR9077164,ERX8620326,ERS10857214,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET2S3,SAMEA13254171,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET2S3 s,Sv2aHET2S3 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET2S3L003-1.fastq.gz,fastq,743488189.0,9842286.0,E MTAB 11505:sv2aHET2S3L003,0:75.54 1:0,A:175979238;C:183726319;G:177933958;T:205735955;N:112719,75,0,,,175979238,183726319,177933958,205735955,112719,ERX8620326,ERS10857214,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.9593,,0.08716,,0.65437,,0.46976,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 10450,ERR9077165,ERX8620326,ERS10857214,ERP135966,PRJEB51339,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E-MTAB-11505,Transcriptome Analysis,Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,,Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Sv2aHET2S3,SAMEA13254171,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg",ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB,,,,,,,,,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,E MTAB 11505:Sv2aHET2S3 s,Sv2aHET2S3 s,RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.,Experimental Factor: genotype:sv2a+/ ,RNA-Seq,TRANSCRIPTOMIC,PolyA,SINGLE,ILLUMINA,NextSeq 500,,ERP135966,NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae,ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21,sv2aHET2S3L004-1.fastq.gz,fastq,747723481.0,9898304.0,E MTAB 11505:sv2aHET2S3L004,0:75.54 1:0,A:176935451;C:184684782;G:179046040;T:206946124;N:111084,75,0,,,176935451,184684782,179046040,206946124,111084,ERX8620326,ERS10857214,ERA9644804,"Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive",1,0.95934,,0.08676,,0.65533,,0.47523,,75,,B,,usable mapping rate,illumina,nextseq,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-04-21,Larval,Larval,Head,Nervous System 11025,ERR9787516,ERX9334792,ERS12088364,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 9,SAMEA14460887,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460887|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:24Z|INSDC last update:2022 09 12T12:19:24Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 9|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 9|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 9 p,Sample 9 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,TSCHOM1_1.fastq.gz TSCHOM1_2.fastq.gz,fastq fastq,6360351174.0,31486887.0,E MTAB 11776:TSCHOM1 ,0:101 1:101,A:1750731340;C:1410426576;G:1460021447;T:1739131898;N:39913,101,101,,,1750731340,1410426576,1460021447,1739131898,39913,ERX9334792,ERS12088364,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93517,0.92417,0.15551,0.15199,0.67736,0.6786,0.48763,0.48988,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11026,ERR9787515,ERX9334791,ERS12088363,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 8,SAMEA14460886,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460886|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:24Z|INSDC last update:2022 09 12T12:19:24Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 8|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 8|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 8 p,Sample 8 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,DEPHOM4_1.fastq.gz DEPHOM4_2.fastq.gz,fastq fastq,6195443828.0,30670514.0,E MTAB 11776:DEPHOM4 ,0:101 1:101,A:1524344281;C:1573560980;G:1585093004;T:1512406843;N:38720,101,101,,,1524344281,1573560980,1585093004,1512406843,38720,ERX9334791,ERS12088363,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.95088,0.9542,0.17631,0.17203,0.71121,0.70981,0.56015,0.57999,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11027,ERR9787514,ERX9334790,ERS12088362,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 7,SAMEA14460885,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460885|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 7|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 7|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 7 p,Sample 7 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,DEPHOM7_1.fastq.gz DEPHOM7_2.fastq.gz,fastq fastq,6201660378.0,30701289.0,E MTAB 11776:DEPHOM7 ,0:101 1:101,A:1699906420;C:1382805098;G:1427335171;T:1691574092;N:39597,101,101,,,1699906420,1382805098,1427335171,1691574092,39597,ERX9334790,ERS12088362,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.92781,0.92603,0.15556,0.15416,0.67965,0.67963,0.48209,0.48229,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11028,ERR9787513,ERX9334789,ERS12088361,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 6,SAMEA14460884,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460884|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 6|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 6 p,Sample 6 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,DEPHOM6_1.fastq.gz DEPHOM6_2.fastq.gz,fastq fastq,6255857382.0,30969591.0,E MTAB 11776:DEPHOM6 ,0:101 1:101,A:1722554517;C:1398134491;G:1424183345;T:1710945949;N:39080,101,101,,,1722554517,1398134491,1424183345,1710945949,39080,ERX9334789,ERS12088361,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93476,0.93206,0.16121,0.1592,0.67882,0.67898,0.48043,0.47851,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11029,ERR9787512,ERX9334788,ERS12088360,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 5,SAMEA14460883,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460883|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 5|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 5 p,Sample 5 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,DEPHOM1_1.fastq.gz DEPHOM1_2.fastq.gz,fastq fastq,6340417006.0,31388203.0,E MTAB 11776:DEPHOM1 ,0:101 1:101,A:1738622221;C:1420060351;G:1455477148;T:1726216833;N:40453,101,101,,,1738622221,1420060351,1455477148,1726216833,40453,ERX9334788,ERS12088360,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93634,0.93167,0.15036,0.14813,0.67809,0.67813,0.47978,0.47851,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11030,ERR9787511,ERX9334787,ERS12088359,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 4,SAMEA14460882,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460882|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 4|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 4 p,Sample 4 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / x tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,2XHOM7_1.fastq.gz 2XHOM7_2.fastq.gz,fastq fastq,6291880446.0,31147923.0,E MTAB 11776:2XHOM7 ,0:101 1:101,A:1712784902;C:1424143534;G:1452943449;T:1701969086;N:39475,101,101,,,1712784902,1424143534,1452943449,1701969086,39475,ERX9334787,ERS12088359,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93869,0.93511,0.15405,0.15042,0.67377,0.67351,0.50599,0.50683,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11031,ERR9787510,ERX9334786,ERS12088358,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 3,SAMEA14460881,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460881|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 3|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 3 p,Sample 3 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / x tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,2XHOM6_1.fastq.gz 2XHOM6_2.fastq.gz,fastq fastq,6214327596.0,30763998.0,E MTAB 11776:2XHOM6 ,0:101 1:101,A:1705019759;C:1384064144;G:1432455427;T:1692749964;N:38302,101,101,,,1705019759,1384064144,1432455427,1692749964,38302,ERX9334786,ERS12088358,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93591,0.92636,0.14492,0.14145,0.67489,0.67491,0.47753,0.49286,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11032,ERR9787509,ERX9334785,ERS12088357,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 2,SAMEA14460880,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460880|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 2|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 2 p,Sample 2 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / x tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,2XHOM4_1.fastq.gz 2XHOM4_2.fastq.gz,fastq fastq,6301691990.0,31196495.0,E MTAB 11776:2XHOM4 ,0:101 1:101,A:1698118705;C:1442276237;G:1473988715;T:1687268823;N:39510,101,101,,,1698118705,1442276237,1473988715,1687268823,39510,ERX9334785,ERS12088357,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.94255,0.93839,0.13232,0.13007,0.67326,0.67294,0.49084,0.49291,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11033,ERR9787508,ERX9334784,ERS12088356,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 16,SAMEA14460879,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460879|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 16|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 16|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 16 p,Sample 16 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,WT7_1.fastq.gz WT7_2.fastq.gz,fastq fastq,6206666746.0,30726073.0,E MTAB 11776:WT7 ,0:101 1:101,A:1705689174;C:1387290312;G:1420249657;T:1693397929;N:39674,101,101,,,1705689174,1387290312,1420249657,1693397929,39674,ERX9334784,ERS12088356,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93493,0.92964,0.15803,0.1554,0.67405,0.67501,0.47988,0.46768,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11034,ERR9787507,ERX9334783,ERS12088355,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 15,SAMEA14460878,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460878|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 15|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 15|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 15 p,Sample 15 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,WT6_1.fastq.gz WT6_2.fastq.gz,fastq fastq,6360740630.0,31488815.0,E MTAB 11776:WT6 ,0:101 1:101,A:1735195643;C:1436805269;G:1469558083;T:1719141699;N:39936,101,101,,,1735195643,1436805269,1469558083,1719141699,39936,ERX9334783,ERS12088355,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93629,0.93343,0.15169,0.1501,0.67572,0.67517,0.47499,0.47879,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11035,ERR9787506,ERX9334782,ERS12088354,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 14,SAMEA14460877,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460877|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 14|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 14|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 14 p,Sample 14 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,WT4_1.fastq.gz WT4_2.fastq.gz,fastq fastq,6227472342.0,30829071.0,E MTAB 11776:WT4 ,0:101 1:101,A:1668352581;C:1434152947;G:1468645621;T:1656281986;N:39207,101,101,,,1668352581,1434152947,1468645621,1656281986,39207,ERX9334782,ERS12088354,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.94261,0.93675,0.15334,0.15049,0.67927,0.67872,0.48762,0.49138,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11036,ERR9787505,ERX9334781,ERS12088353,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 13,SAMEA14460876,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460876|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 13|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 13|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 13 p,Sample 13 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:wild type genotype,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,WT1_1.fastq.gz WT1_2.fastq.gz,fastq fastq,6332757570.0,31350285.0,E MTAB 11776:WT1 ,0:101 1:101,A:1726838066;C:1431452165;G:1460746594;T:1713681043;N:39702,101,101,,,1726838066,1431452165,1460746594,1713681043,39702,ERX9334781,ERS12088353,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.92422,0.93556,0.14884,0.14926,0.67694,0.67564,0.48223,0.48884,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11037,ERR9787504,ERX9334780,ERS12088352,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 12,SAMEA14460875,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460875|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 12|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 12|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 12 p,Sample 12 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,TSCHOM7_1.fastq.gz TSCHOM7_2.fastq.gz,fastq fastq,6216990360.0,30777180.0,E MTAB 11776:TSCHOM7 ,0:101 1:101,A:1710256020;C:1390027205;G:1419250792;T:1697417099;N:39244,101,101,,,1710256020,1390027205,1419250792,1697417099,39244,ERX9334780,ERS12088352,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93162,0.92801,0.15191,0.14943,0.67789,0.67951,0.47915,0.47762,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11038,ERR9787503,ERX9334779,ERS12088351,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 11,SAMEA14460874,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460874|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 11|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 11|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 11 p,Sample 11 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,TSCHOM6_1.fastq.gz TSCHOM6_2.fastq.gz,fastq fastq,6199081848.0,30688524.0,E MTAB 11776:TSCHOM6 ,0:101 1:101,A:1705859045;C:1387530558;G:1415224718;T:1690428701;N:38826,101,101,,,1705859045,1387530558,1415224718,1690428701,38826,ERX9334779,ERS12088351,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93583,0.93349,0.15923,0.15664,0.67976,0.67986,0.48632,0.48376,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11039,ERR9787502,ERX9334778,ERS12088350,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 10,SAMEA14460873,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460873|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 10|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 10|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 10 p,Sample 10 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,TSCHOM4_1.fastq.gz TSCHOM4_2.fastq.gz,fastq fastq,6287836204.0,31127902.0,E MTAB 11776:TSCHOM4 ,0:101 1:101,A:1673762523;C:1453880149;G:1493343671;T:1666809849;N:40012,101,101,,,1673762523,1453880149,1493343671,1666809849,40012,ERX9334778,ERS12088350,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.94269,0.9375,0.14166,0.13979,0.67476,0.67722,0.4927,0.49365,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11040,ERR9787501,ERX9334777,ERS12088349,ERP137937,PRJEB53153,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E-MTAB-11776,Transcriptome Analysis,Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae.,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,,Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Sample 1,SAMEA14460872,"Laboratory for Molecular Biodiscovery, KU Leuven",ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460872|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 1|scientific name:Danio rerio|sex:non available|strain:AB,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,E MTAB 11776:Sample 1 p,Sample 1 p,RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.,Experimental Factor: genotype:depdc5 / x tsc2 / ,RNA-Seq,TRANSCRIPTOMIC,PolyA,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP137937,Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae,ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12,2XHOM1_1.fastq.gz 2XHOM1_2.fastq.gz,fastq fastq,6232605768.0,30854484.0,E MTAB 11776:2XHOM1 ,0:101 1:101,A:1725401812;C:1384507160;G:1410306583;T:1712351429;N:38784,101,101,,,1725401812,1384507160,1410306583,1712351429,38784,ERX9334777,ERS12088349,ERA14756702,"Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive","Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive",2,0.93594,0.93262,0.15282,0.14977,0.67503,0.67373,0.49522,0.49759,101,101,B,B,biological fallback assumption,illumina,novaseq_era,unknown,poly_a,trueseq,bulk,unknown,unknown,,Belgium,2022-09-12,Larval,Larval,Head,Nervous System 11047,ERR10476807,ERX9997150,ERS13672475,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day6,SAMEA111562619,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d6 1|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star pos d6 1|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:183 283033,Sample 0256 076 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_076_FR_NSP_TR1_SL1_S7_L001_R1_001-pooled.fastq.gz 0256_076_FR_NSP_TR1_SL1_S7_L001_R2_001-pooled.fastq.gz,fastq fastq,2974117224.0,29158012.0,ena RUN TAB 09 11 2022 11:52:59:183 283034,0:51 1:51,A:816102587;C:662715848;G:672777315;T:822487687;N:33787,51,51,,,816102587,662715848,672777315,822487687,33787,ERX9997150,ERS13672475,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11050,ERR10476814,ERX9997157,ERS13672482,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day13,SAMEA111562626,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d13 3|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star pos d13 3|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:186 283047,Sample 0256 083 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_083_FR_NSP_TR2_SL1_S14_L001_R1_001-pooled.fastq.gz 0256_083_FR_NSP_TR2_SL1_S14_L001_R2_001-pooled.fastq.gz,fastq fastq,5492667156.0,53849678.0,ena RUN TAB 09 11 2022 11:52:59:186 283048,0:51 1:51,A:1546610275;C:1178241230;G:1195797489;T:1571955787;N:62375,51,51,,,1546610275,1178241230,1195797489,1571955787,62375,ERX9997157,ERS13672482,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11051,ERR10476829,ERX9997172,ERS13672497,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day6,SAMEA111562641,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d6 3|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star neg d6 3|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:192 283077,Sample 0256 103 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_103_FR_NSP_TR1_SL1_S32_L001_R1_001-pooled.fastq.gz 0256_103_FR_NSP_TR1_SL1_S32_L001_R2_001-pooled.fastq.gz,fastq fastq,3120070248.0,30588924.0,ena RUN TAB 09 11 2022 11:52:59:193 283078,0:51 1:51,A:846274026;C:707353227;G:707836608;T:858570820;N:35567,51,51,,,846274026,707353227,707836608,858570820,35567,ERX9997172,ERS13672497,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11053,ERR10476796,ERX9997139,ERS13672464,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day13,SAMEA111562608,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d13 5|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:tu wt d13 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:178 283011,Sample 0256 010 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_010_FR_NSP_TR1_SL1_S21_L001_R1_001-pooled.fastq.gz 0256_010_FR_NSP_TR1_SL1_S21_L001_R2_001-pooled.fastq.gz,fastq fastq,2969687772.0,29114586.0,ena RUN TAB 09 11 2022 11:52:59:179 283012,0:51 1:51,A:762435544;C:713336460;G:731717998;T:762096042;N:101728,51,51,,,762435544,713336460,731717998,762096042,101728,ERX9997139,ERS13672464,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11055,ERR10476836,ERX9997179,ERS13672504,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day13,SAMEA111562648,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d13 5|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star neg d13 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:195 283091,Sample 0256 110 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_110_FR_NSP_TR2_SL1_S39_L001_R1_001-pooled.fastq.gz 0256_110_FR_NSP_TR2_SL1_S39_L001_R2_001-pooled.fastq.gz,fastq fastq,2758547160.0,27044580.0,ena RUN TAB 09 11 2022 11:52:59:196 283092,0:51 1:51,A:732517446;C:640766914;G:643632251;T:741598802;N:31747,51,51,,,732517446,640766914,643632251,741598802,31747,ERX9997179,ERS13672504,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11056,ERR10476808,ERX9997151,ERS13672476,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day6,SAMEA111562620,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d6 2|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star pos d6 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:184 283035,Sample 0256 077 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_077_FR_NSP_TR1_SL1_S8_L001_R1_001-pooled.fastq.gz 0256_077_FR_NSP_TR1_SL1_S8_L001_R2_001-pooled.fastq.gz,fastq fastq,3595088328.0,35245964.0,ena RUN TAB 09 11 2022 11:52:59:184 283036,0:51 1:51,A:985480827;C:803117311;G:811895458;T:994553698;N:41034,51,51,,,985480827,803117311,811895458,994553698,41034,ERX9997151,ERS13672476,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11058,ERR10476827,ERX9997170,ERS13672495,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day6,SAMEA111562639,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d6 1|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star neg d6 1|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:192 283073,Sample 0256 101 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_101_FR_NSP_TR1_SL1_S30_L001_R1_001-pooled.fastq.gz 0256_101_FR_NSP_TR1_SL1_S30_L001_R2_001-pooled.fastq.gz,fastq fastq,3504125952.0,34354176.0,ena RUN TAB 09 11 2022 11:52:59:192 283074,0:51 1:51,A:947138693;C:797358353;G:798903844;T:960685108;N:39954,51,51,,,947138693,797358353,798903844,960685108,39954,ERX9997170,ERS13672495,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11060,ERR10476794,ERX9997137,ERS13672462,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day13,SAMEA111562606,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d13 3|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:tu wt d13 3|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:178 283007,Sample 0256 008 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_008_FR_NSP_TR1_SL1_S1_L001_R1_001-pooled.fastq.gz 0256_008_FR_NSP_TR1_SL1_S1_L001_R2_001-pooled.fastq.gz,fastq fastq,2659023618.0,26068859.0,ena RUN TAB 09 11 2022 11:52:59:178 283008,0:51 1:51,A:670947753;C:637136773;G:677570266;T:673338670;N:30156,51,51,,,670947753,637136773,677570266,673338670,30156,ERX9997137,ERS13672462,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11061,ERR10476833,ERX9997176,ERS13672501,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day13,SAMEA111562645,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d13 2|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star neg d13 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:194 283085,Sample 0256 107 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_107_FR_NSP_TR2_SL1_S36_L001_R1_001-pooled.fastq.gz 0256_107_FR_NSP_TR2_SL1_S36_L001_R2_001-pooled.fastq.gz,fastq fastq,3282322362.0,32179631.0,ena RUN TAB 09 11 2022 11:52:59:194 283086,0:51 1:51,A:898607442;C:734331926;G:739939203;T:909406172;N:37619,51,51,,,898607442,734331926,739939203,909406172,37619,ERX9997176,ERS13672501,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11063,ERR10476828,ERX9997171,ERS13672496,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day6,SAMEA111562640,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d6 2|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star neg d6 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:192 283075,Sample 0256 102 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_102_FR_NSP_TR1_SL1_S31_L001_R1_001-pooled.fastq.gz 0256_102_FR_NSP_TR1_SL1_S31_L001_R2_001-pooled.fastq.gz,fastq fastq,3969901404.0,38920602.0,ena RUN TAB 09 11 2022 11:52:59:192 283076,0:51 1:51,A:1079447747;C:894502841;G:908706945;T:1087198510;N:45361,51,51,,,1079447747,894502841,908706945,1087198510,45361,ERX9997171,ERS13672496,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11068,ERR10476790,ERX9997133,ERS13672458,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day6,SAMEA111562602,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d6 4|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:tu wt d6 4|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:176 282999,Sample 0256 004 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_004_FR_NSP_TR1_SL1_S16_L001_R1_001-pooled.fastq.gz 0256_004_FR_NSP_TR1_SL1_S16_L001_R2_001-pooled.fastq.gz,fastq fastq,2693489520.0,26406760.0,ena RUN TAB 09 11 2022 11:52:59:176 283000,0:51 1:51,A:701762127;C:639663261;G:643729664;T:708242984;N:91484,51,51,,,701762127,639663261,643729664,708242984,91484,ERX9997133,ERS13672458,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11070,ERR10476791,ERX9997134,ERS13672459,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day6,SAMEA111562603,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d6 5|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:tu wt d6 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:176 283001,Sample 0256 005 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_005_FR_NSP_TR1_SL1_S17_L001_R1_001-pooled.fastq.gz 0256_005_FR_NSP_TR1_SL1_S17_L001_R2_001-pooled.fastq.gz,fastq fastq,2904430620.0,28474810.0,ena RUN TAB 09 11 2022 11:52:59:176 283002,0:51 1:51,A:777892611;C:666758312;G:672026589;T:787654140;N:98968,51,51,,,777892611,666758312,672026589,787654140,98968,ERX9997134,ERS13672459,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11074,ERR10476788,ERX9997131,ERS13672456,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,wildtype whole brain pooled 15 brains,wildtype whole brain day6,SAMEA111562600,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:tu wt d6 2|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:tu wt d6 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:175 282995,Sample 0256 002 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_002_FR_NSP_TR1_SL1_S14_L001_R1_001-pooled.fastq.gz 0256_002_FR_NSP_TR1_SL1_S14_L001_R2_001-pooled.fastq.gz,fastq fastq,2385303456.0,23385328.0,ena RUN TAB 09 11 2022 11:52:59:175 282996,0:51 1:51,A:620601269;C:567608030;G:568555174;T:628457831;N:81152,51,51,,,620601269,567608030,568555174,628457831,81152,ERX9997131,ERS13672456,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11077,ERR10476816,ERX9997159,ERS13672484,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day13,SAMEA111562628,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d13 5|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star pos d13 5|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:187 283051,Sample 0256 085 FR NSP TRP SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_085_FR_NSP_TRP_SL1_S16_L001_R1_001-pooled.fastq.gz 0256_085_FR_NSP_TRP_SL1_S16_L001_R2_001-pooled.fastq.gz,fastq fastq,3264856596.0,32008398.0,ena RUN TAB 09 11 2022 11:52:59:187 283052,0:51 1:51,A:908889213;C:708892816;G:727180747;T:919857088;N:36732,51,51,,,908889213,708892816,727180747,919857088,36732,ERX9997159,ERS13672484,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11078,ERR10476813,ERX9997156,ERS13672481,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day13,SAMEA111562625,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d13 2|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star pos d13 2|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:186 283045,Sample 0256 082 FR NSP TR2 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_082_FR_NSP_TR2_SL1_S13_L001_R1_001-pooled.fastq.gz 0256_082_FR_NSP_TR2_SL1_S13_L001_R2_001-pooled.fastq.gz,fastq fastq,3037654248.0,29780924.0,ena RUN TAB 09 11 2022 11:52:59:186 283046,0:51 1:51,A:859112641;C:645943863;G:660257800;T:872305592;N:34352,51,51,,,859112641,645943863,660257800,872305592,34352,ERX9997156,ERS13672481,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11079,ERR10476832,ERX9997175,ERS13672500,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC / whole brain pooled 15 brains,star:bPAC / whole brain day13,SAMEA111562644,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star neg d13 1|collection date:2019 12|common name:zebrafish|dev stage:13 dpf|geographic location country and/or sea:Germany|sample name:star neg d13 1|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:194 283083,Sample 0256 106 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_106_FR_NSP_TR1_SL1_S35_L001_R1_001-pooled.fastq.gz 0256_106_FR_NSP_TR1_SL1_S35_L001_R2_001-pooled.fastq.gz,fastq fastq,2761703040.0,27075520.0,ena RUN TAB 09 11 2022 11:52:59:194 283084,0:51 1:51,A:761065450;C:610246142;G:622228689;T:768131440;N:31319,51,51,,,761065450,610246142,622228689,768131440,31319,ERX9997175,ERS13672500,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System 11080,ERR10476810,ERX9997153,ERS13672478,ERP138527,PRJEB53713,Time course whole brain transcriptome and methylome profiles of early life high GC exposed zebrafish,f79d57b2-1e93-4ca0-8fda-fd881a2a0c1e,Other,Whole brain transcriptome mRNA profiles of zebrafish which exposed to elavated glucocorticoid GC optogenetically at 6 13 120 dpf dpf and post predatory stress looming dots stress at xxx dpf. All subjective adults at 120 dpf were female. Tubingen strain was used as wildtype control and brains from transgenic line Tgstar:bPAC 2A tdTomatouoe300 +/ and / were sequenced on Illumina NovaSeq6000 by TRON gGmbH Mainz Germany. Paired end TruSeq Stranded mRNA libraries Illumina CA USA were constructed and over 20M of 50 bp reads/sample were sequenced. Oxford Nanopore sequencing was used for DNA methylation identification. These data were generated for the study by Choi et al. 2024. https://www.biorxiv.org/content/10.1101/2023.02.13.528363v4,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,,star:bPAC+/ whole brain pooled 15 brains,star:bPAC+/ whole brain day6,SAMEA111562622,"Living Systems Institute, University of Exeter",INSDC center name:Living Systems Institute University of Exeter|Submitter Id:star pos d6 4|collection date:2019 12|common name:zebrafish|dev stage:6 dpf|geographic location country and/or sea:Germany|sample name:star pos d6 4|scientific name:Danio rerio|sex:N/A|strain:Tübingen|tissue type:brain,,,,,,,,,Illumina NovaSeq 6000 paired end sequencing,ena EXPERIMENT TAB 09 11 2022 11:52:59:184 283039,Sample 0256 079 FR NSP TR1 SL1,1,,,RNA-Seq,TRANSCRIPTOMIC,Oligo-dT,PAIRED,ILLUMINA,Illumina NovaSeq 6000,,ERP138527,Illumina NovaSeq 6000 paired end sequencing,ENA FIRST PUBLIC:2024 02 06|ENA LAST UPDATE:2024 02 06,0256_079_FR_NSP_TR1_SL1_S10_L001_R1_001-pooled.fastq.gz 0256_079_FR_NSP_TR1_SL1_S10_L001_R2_001-pooled.fastq.gz,fastq fastq,2745379674.0,26915487.0,ena RUN TAB 09 11 2022 11:52:59:185 283040,0:51 1:51,A:762974242;C:602287296;G:611894421;T:768192503;N:31212,51,51,,,762974242,602287296,611894421,768192503,31212,ERX9997153,ERS13672478,ERA18581410,"living systems institute, university of exeter|European Nucleotide Archive","living systems institute, university of exeter|European Nucleotide Archive",,,,,,,,,,,,B,B,biological fallback assumption,illumina,novaseq_era,full_length,poly_a,trueseq,bulk,unknown,unknown,,United Kingdom,2024-02-06,Larval,Larval,Brain,Nervous System