rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
36478,SRR527835,SRX171222,SRS352108,SRP014596,PRJNA171539,Transcriptomic analysis of zebrafish during development and homeostasis,GSE39703,Transcriptome Analysis,Sequencing libraries were generated from total RNA samples following the mRNAseq protocol for the generation of single end 16 hpf 36 hpf  5 day larvae  adult head and adult tail or paired end 24 hpf libraries Illumina. Single end reads of 36 nucleotides and paired end reads 2 x 76 nucleotides were obtained with a GAIIx Illumina. Gene expression at the different stages/tissu was assessed by cufflinks and HTseq. Overall design: RNAseq on 5 differents samples:  24hpf embryos  pool of 16 hour to 36 hour embryos  5 dpf larvea  adult head and adult tail,,pubmed:23684812,,tail,GSM977960,,tissue:entire adult tail|genotype:Wild type|strain:AB|Stage:adult tail,tail,Basecalls performed using CASAVA version 1.4 Reads aligned to zebrafish genome Zv9 Ensembl with Tophat v 1.4.1 and Bowtie v 0.12.7  options: butterfly search coverage search  microexon search  min anchor length 5   G GTF Determination of raw reads aligned to ZV9 with HTSeq v0.5.3p3 and gtf file ensembl zv9 release 60 Determination of RPKM/FPKM with cufflinks v 1.3.0 with the options  u  b  M rRNA/Mtgenes mask  G gtf Genome build: Zv9 v 60 Supplementary files format and content: *.count file are the output of Htseq reads quantification. *.fpkm files are the output of cufflinks quantification,entire adult tail,,Extraction of total RNA with Trizol following manufacturer's instruction. Generation of mRNA libraries with Trueseq RNA kit following Illumina's instructions,,genotype:Wild type|strain:AB|Stage:adult tail,GSM977960,GSM977960: tail; Danio rerio; RNA Seq,GSM977960 1,GSM977960: tail,1,,GEO Accession:GSM977960,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina Genome Analyzer IIx,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,SRP014596,,,,,534897504.0,14858264.0,GSM977960 r1,0:36,A:144900139;C:122639505;G:130189210;T:136990883;N:177767,36,,,,144900139,122639505,130189210,136990883,177767,SRX171222,SRS352108,SRA056408,GEO,ITG,1,0.89933,,0.04578,,0.71254,,0.41668,,36,,B,,usable mapping rate,illumina,early_illumina,unknown,cdna_unspecified,trueseq,bulk,unknown,unknown,,Unknown,2012-07-27,Adult,Adult,Tail,Multi-system
41418,SRR4423118,SRX2245302,SRS1745860,SRP091534,PRJNA347637,Danio rerio and Xenopus laevis Raw sequence reads,PRJNA347637,Other,To study the transcriptome during development,,,,,Adult FT Dr,,strain:not applicable|isolate:not applicable|breed:ABTL|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:Adult|sex:female|tissue:tail|BioSampleModel:Model organism or animal,,,,,,,,,miRNA Seq of Zebrafish: Adult female tail,172 5,172 5,Libraries were generated according to the manufacturers’ protocols using the Ion Total RNA Seq Kit v2 and the Ion Xpress™ RNA Seq bar coding kit Thermo Fisher Scientific. A few modifications were made during purification steps in the small RNAseq to allow sequencing of longer RNAs up to 200 nt than with standard sRNA protocols. Namely: 153 µl ethanol instead of 120 µl during purification of cDNA and 134 µl ethanol instead of 110 µl in protocol.,,,miRNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,SRP091534,,,femaletailRID0172BC05smallZF.fastq,fastq,491055734.0,7690105.0,femaletailRID0172BC05smallZF.fastq,0:63.86,A:114732043;C:132287469;G:130972143;T:113064079;N:0,63,,,,114732043,132287469,130972143,113064079,0,SRX2245302,SRS1745860,SRA485147,Universiteit van Amsterdam|SILS,Universiteit van Amsterdam,1,0.82246,,0.13521,,0.91303,,0.63226,,43,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,small_rna,unknown,bulk,unknown,unknown,,Netherlands,2016-10-19,Adult,Adult,Tail,Multi-system
41419,SRR4423117,SRX2245301,SRS1745860,SRP091534,PRJNA347637,Danio rerio and Xenopus laevis Raw sequence reads,PRJNA347637,Other,To study the transcriptome during development,,,,,Adult FT Dr,,strain:not applicable|isolate:not applicable|breed:ABTL|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:Adult|sex:female|tissue:tail|BioSampleModel:Model organism or animal,,,,,,,,,RNA Seq of Zebrafish: Adult female tail,172 3,172 3,Libraries were generated according to the manufacturers’ protocols using the Ion Total RNA Seq Kit v2 and the Ion Xpress™ RNA Seq bar coding kit Thermo Fisher Scientific.,,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ION_TORRENT,Ion Torrent Proton,,SRP091534,,,femaletailRID0172BC03largeZF.fastq,fastq,1414932187.0,9757587.0,femaletailRID0172BC03largeZF.fastq,0:145.01,A:343007105;C:362895564;G:422320111;T:286709407;N:0,145,,,,343007105,362895564,422320111,286709407,0,SRX2245301,SRS1745860,SRA485147,Universiteit van Amsterdam|SILS,Universiteit van Amsterdam,1,0.96057,,0.21796,,0.95177,,0.76663,,141,,B,,usable mapping rate,ion_torrent,ion_torrent,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Netherlands,2016-10-19,Adult,Adult,Tail,Multi-system