rowid,run.accession,experiment.accession,sample.accession,study.accession,bioproject,study.title,study.alias,study.type,study.abstract,study.attributes,study.PMIDs,sample.description,sample.title,sample.alias,sample.centername,sample.attributes,GEOsample.title,GEOsample.dataprocessing,GEOsample.source,GEOsample.treatmentprotocol,GEOsample.extractprotocol,GEOsample.growthprotocol,GEOsample.characteristics,GEOsample.accession,experiment.title,experiment.alias,experiment.library_name,experiment.design_description,experiment.library_construction_protocol,experiment.attributes,experiment.library_strategy,experiment.library_source,experiment.library_selection,experiment.library_layout,experiment.platform,experiment.instrument_model,experiment.spot_descriptor,experiment.study_ref,run.title,run.attributes,run.filename,run.semantic_name,run.total_bases,run.total_spots,run.alias,run.read_lengths,run.base_counts,run.r1_length,run.r2_length,run.r3_length,run.r4_length,run.Acount,run.Ccount,run.Gcount,run.Tcount,run.Ncount,run.experiment,run.pool_member,submission.accession,submission.srasource,submission.bioprojectsource,seqdetective.n_mates,seqdetective.mapping_rate.mate1,seqdetective.mapping_rate.mate2,seqdetective.nofeature_rate.mate1,seqdetective.nofeature_rate.mate2,seqdetective.sparsity.mate1,seqdetective.sparsity.mate2,seqdetective.pos_strand_rate.mate1,seqdetective.pos_strand_rate.mate2,seqdetective.readlen.mate1,seqdetective.readlen.mate2,seqdetective.judgement.mate1,seqdetective.judgement.mate2,seqdetective.judgement.reason,platform_family,instrument_generation,read_bias,selection_class,prep_kit,sc_or_bulk,tech_class,technology,tech_variant,submission.bioprojectsource.country,earliest_date,devstage_curation,devstage_curation_coarse,tissue_curation,tissue_curation_coarse
51,DRR029943,DRX026961,DRS086501,DRP004756,PRJDB3475,Identification of ovulation inducing genes selected by the method for in vivo induction of oocyte maturation and ovulation in zebrafish,DRP004756,Other,To identify ovulation inducing genes  RNAseq analysis was carried out using samples prepared by in vivo assay system in zebrafish.,,,oocyte maturation duirng natural paring,zebrafish ovary isolated from adult fish at oocyte maturation duirng natural paring. [RNAseq],SAMD00025433,,sample name:5 OM|strain:roy|tissue type:ovary|dev stage:adult,,,,,,,,,Illumina HiSeq 2500 sequencing of SAMD00025433,DRX026961,5 OM,1,1,,RNA-Seq,TRANSCRIPTOMIC,cDNA,SINGLE,ILLUMINA,Illumina HiSeq 2500,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,DRP004756,Illumina HiSeq 2500 sequencing of SAMD00025433,,,,573617016.0,15933806.0,DRR029943,0:36,A:130606804;C:141376330;G:145891720;T:155734276;N:7886,36,,,,130606804,141376330,145891720,155734276,7886,DRX026961,DRS086501,DRA003031,SHIZUOKA|Shizuoka University,Shizuoka University,1,0.91108,,0.0173,,0.76205,,0.46319,,36,,B,,usable mapping rate,illumina,hiseq_era,unknown,cdna_unspecified,unknown,bulk,unknown,unknown,,Japan,2019-01-23,Adult,Adult,Multi-tissue,Multi-system
3703,ERR1397087,ERX1468346,ERS1023493,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 186 1 8,SAMEA3716344,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   9 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716344|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:44Z|INSDC status:public|Submitter Id:2362a210 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 9 mpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TCTCTTCA is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph186|sample name:2362a210 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#56,15566396,Illumina sequencing of library 15566396  constructed from sample accession ERS1023493 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCTCTTCA.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#56.cram,cram,707144490.0,5439573.0,SC RUN 18715 8#56,0:55 1:75,A:188121373;C:125159654;G:138365161;T:255495006;N:3296,55,75,,,188121373,125159654,138365161,255495006,3296,ERX1468346,ERS1023493,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.25954,0.84737,0.1072,0.10684,0.96889,0.90337,0.7843,0.68587,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3704,ERR1397086,ERX1468345,ERS1023492,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 186 1 7,SAMEA3716343,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   9 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716343|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:43Z|INSDC status:public|Submitter Id:2359a160 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 9 mpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TGTGAAGA is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph186|sample name:2359a160 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#55,15566395,Illumina sequencing of library 15566395  constructed from sample accession ERS1023492 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGTGAAGA.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#55.cram,cram,618409870.0,4756999.0,SC RUN 18715 8#55,0:55 1:75,A:169528505;C:106711234;G:111143607;T:231024694;N:1830,55,75,,,169528505,106711234,111143607,231024694,1830,ERX1468345,ERS1023492,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.31798,0.81356,0.1471,0.11489,0.9707,0.89708,0.48844,0.54567,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3705,ERR1397085,ERX1468344,ERS1023491,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 186 1 6,SAMEA3716342,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   9 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716342|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:43Z|INSDC status:public|Submitter Id:2350a0b0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 9 mpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TAGACGGA is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph186|sample name:2350a0b0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#54,15566394,Illumina sequencing of library 15566394  constructed from sample accession ERS1023491 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TAGACGGA.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#54.cram,cram,714746240.0,5498048.0,SC RUN 18715 8#54,0:55 1:75,A:192003042;C:125908202;G:130551705;T:266281416;N:1875,55,75,,,192003042,125908202,130551705,266281416,1875,ERX1468344,ERS1023491,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28492,0.84395,0.14733,0.11571,0.9727,0.90149,0.81768,0.65432,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3706,ERR1397084,ERX1468343,ERS1023490,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 186 1 5,SAMEA3716341,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   9 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716341|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:43Z|INSDC status:public|Submitter Id:234778f0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 9 mpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TGCTGATA is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph186|sample name:234778f0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#53,15566393,Illumina sequencing of library 15566393  constructed from sample accession ERS1023490 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGCTGATA.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#53.cram,cram,746119400.0,5739380.0,SC RUN 18715 8#53,0:55 1:75,A:202741777;C:128874768;G:133432494;T:281067099;N:3262,55,75,,,202741777,128874768,133432494,281067099,3262,ERX1468343,ERS1023490,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28545,0.827,0.17591,0.11992,0.97165,0.89483,0.77417,0.62382,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3707,ERR1397083,ERX1468342,ERS1023489,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 186 1 4,SAMEA3716340,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   9 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716340|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:42Z|INSDC status:public|Submitter Id:233e7840 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 9 mpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TCGTTAGC is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph186|sample name:233e7840 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#52,15566392,Illumina sequencing of library 15566392  constructed from sample accession ERS1023489 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCGTTAGC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#52.cram,cram,776598680.0,5973836.0,SC RUN 18715 8#52,0:55 1:75,A:207406716;C:137168865;G:146112068;T:285908651;N:2380,55,75,,,207406716,137168865,146112068,285908651,2380,ERX1468342,ERS1023489,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.27793,0.8492,0.1319,0.11351,0.97145,0.90366,0.80649,0.66871,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3708,ERR1397082,ERX1468341,ERS1023488,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 186 1 3,SAMEA3716339,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   9 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716339|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:42Z|INSDC status:public|Submitter Id:23355080 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 9 mpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TTACTCGC is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph186|sample name:23355080 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#51,15566391,Illumina sequencing of library 15566391  constructed from sample accession ERS1023488 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTACTCGC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#51.cram,cram,636459330.0,4895841.0,SC RUN 18715 8#51,0:55 1:75,A:170841699;C:107415456;G:118061456;T:240137938;N:2781,55,75,,,170841699,107415456,118061456,240137938,2781,ERX1468341,ERS1023488,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.33457,0.84812,0.16722,0.11863,0.97165,0.88755,0.82802,0.54829,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3709,ERR1397081,ERX1468340,ERS1023487,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 186 1 2,SAMEA3716338,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   9 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716338|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:41Z|INSDC status:public|Submitter Id:232c01b0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 9 mpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TATGTGGC is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph186|sample name:232c01b0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#50,15566390,Illumina sequencing of library 15566390  constructed from sample accession ERS1023487 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TATGTGGC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#50.cram,cram,762887060.0,5868362.0,SC RUN 18715 8#50,0:55 1:75,A:205092929;C:134351479;G:140981680;T:282458912;N:2060,55,75,,,205092929,134351479,140981680,282458912,2060,ERX1468340,ERS1023487,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.29975,0.85617,0.14999,0.11598,0.97023,0.8981,0.81631,0.69434,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3710,ERR1397080,ERX1468339,ERS1023486,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 186 1 1,SAMEA3716337,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   9 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716337|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:41Z|INSDC status:public|Submitter Id:2322d9f0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 9 mpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TGTCTATC is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph186|sample name:2322d9f0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#49,15566389,Illumina sequencing of library 15566389  constructed from sample accession ERS1023486 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGTCTATC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#49.cram,cram,654882020.0,5037554.0,SC RUN 18715 8#49,0:55 1:75,A:176142868;C:110275594;G:118303230;T:250156724;N:3604,55,75,,,176142868,110275594,118303230,250156724,3604,ERX1468339,ERS1023486,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.33949,0.8483,0.176,0.09714,0.97542,0.90749,0.84111,0.67388,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3711,ERR1397079,ERX1468338,ERS1023485,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 24,SAMEA3716336,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716336|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:40Z|INSDC status:public|Submitter Id:2319d940 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TTCAGCTC is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:2319d940 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#48,15566388,Illumina sequencing of library 15566388  constructed from sample accession ERS1023485 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTCAGCTC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#48.cram,cram,677343030.0,5210331.0,SC RUN 18715 8#48,0:55 1:75,A:177744951;C:121003073;G:129268553;T:249323382;N:3071,55,75,,,177744951,121003073,129268553,249323382,3071,ERX1468338,ERS1023485,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.27162,0.86035,0.10137,0.08341,0.97573,0.91376,0.82856,0.62728,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3712,ERR1397078,ERX1468337,ERS1023484,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 23,SAMEA3716335,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716335|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:40Z|INSDC status:public|Submitter Id:2310b180 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TACTAGTC is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:2310b180 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#47,15566387,Illumina sequencing of library 15566387  constructed from sample accession ERS1023484 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TACTAGTC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#47.cram,cram,756384720.0,5818344.0,SC RUN 18715 8#47,0:55 1:75,A:204421756;C:131322874;G:139056982;T:281579686;N:3422,55,75,,,204421756,131322874,139056982,281579686,3422,ERX1468337,ERS1023484,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.31847,0.85466,0.13579,0.08314,0.97559,0.91484,0.85907,0.69484,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3713,ERR1397077,ERX1468336,ERS1023483,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 22,SAMEA3716334,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716334|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:39Z|INSDC status:public|Submitter Id:2307b0d0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TCAGATTC is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:2307b0d0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#46,15566386,Illumina sequencing of library 15566386  constructed from sample accession ERS1023483 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCAGATTC.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#46.cram,cram,736276970.0,5663669.0,SC RUN 18715 8#46,0:55 1:75,A:195907677;C:131386929;G:137210683;T:271768338;N:3343,55,75,,,195907677,131386929,137210683,271768338,3343,ERX1468336,ERS1023483,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.25731,0.83896,0.10708,0.0752,0.97725,0.91829,0.82893,0.68981,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3714,ERR1397076,ERX1468335,ERS1023482,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 21,SAMEA3716333,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716333|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:39Z|INSDC status:public|Submitter Id:22feb020 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TATGCCAG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22feb020 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#45,15566385,Illumina sequencing of library 15566385  constructed from sample accession ERS1023482 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TATGCCAG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#45.cram,cram,666434860.0,5126422.0,SC RUN 18715 8#45,0:55 1:75,A:175545494;C:119084096;G:124317188;T:247486133;N:1949,55,75,,,175545494,119084096,124317188,247486133,1949,ERX1468335,ERS1023482,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28297,0.85288,0.13833,0.10107,0.97534,0.90991,0.80466,0.52355,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3715,ERR1397075,ERX1468334,ERS1023481,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 20,SAMEA3716332,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716332|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:38Z|INSDC status:public|Submitter Id:22f5af70 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TGGCTCAG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22f5af70 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#44,15566384,Illumina sequencing of library 15566384  constructed from sample accession ERS1023481 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGGCTCAG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#44.cram,cram,695152510.0,5347327.0,SC RUN 18715 8#44,0:55 1:75,A:184288447;C:123186272;G:129742627;T:257932830;N:2334,55,75,,,184288447,123186272,129742627,257932830,2334,ERX1468334,ERS1023481,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.31006,0.8513,0.12966,0.09655,0.97461,0.91423,0.8429,0.69582,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3716,ERR1397074,ERX1468333,ERS1023480,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 19,SAMEA3716331,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716331|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:38Z|INSDC status:public|Submitter Id:22ecaec0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TCATTGAG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22ecaec0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#43,15566383,Illumina sequencing of library 15566383  constructed from sample accession ERS1023480 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCATTGAG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#43.cram,cram,533568230.0,4104371.0,SC RUN 18715 8#43,0:55 1:75,A:143539058;C:96004871;G:98641156;T:195381631;N:1514,55,75,,,143539058,96004871,98641156,195381631,1514,ERX1468333,ERS1023480,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.2816,0.83134,0.10829,0.09548,0.97463,0.91463,0.82774,0.63791,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3717,ERR1397073,ERX1468332,ERS1023479,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 18,SAMEA3716330,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716330|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:37Z|INSDC status:public|Submitter Id:22e3ae10 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TGTATGCG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22e3ae10 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#42,15566382,Illumina sequencing of library 15566382  constructed from sample accession ERS1023479 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGTATGCG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#42.cram,cram,661945700.0,5091890.0,SC RUN 18715 8#42,0:55 1:75,A:179311956;C:118843676;G:120891014;T:242897159;N:1895,55,75,,,179311956,118843676,120891014,242897159,1895,ERX1468332,ERS1023479,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.29346,0.84765,0.13347,0.11035,0.97303,0.91295,0.80889,0.64077,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3718,ERR1397072,ERX1468331,ERS1023478,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 17,SAMEA3716329,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716329|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:37Z|INSDC status:public|Submitter Id:22daad60 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TCCAGTCG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22daad60 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#41,15566381,Illumina sequencing of library 15566381  constructed from sample accession ERS1023478 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCCAGTCG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#41.cram,cram,665846740.0,5121898.0,SC RUN 18715 8#41,0:55 1:75,A:176220973;C:118768091;G:124615863;T:246239820;N:1993,55,75,,,176220973,118768091,124615863,246239820,1993,ERX1468331,ERS1023478,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28802,0.86238,0.12173,0.09559,0.97262,0.90765,0.80726,0.66136,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3719,ERR1397071,ERX1468330,ERS1023477,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 16,SAMEA3716328,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716328|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:36Z|INSDC status:public|Submitter Id:22d1acb0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TAAGTTCG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22d1acb0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#40,15566380,Illumina sequencing of library 15566380  constructed from sample accession ERS1023477 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TAAGTTCG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#40.cram,cram,619781760.0,4767552.0,SC RUN 18715 8#40,0:55 1:75,A:166468116;C:111775459;G:114959803;T:226576853;N:1529,55,75,,,166468116,111775459,114959803,226576853,1529,ERX1468330,ERS1023477,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.27098,0.83733,0.10627,0.08837,0.97457,0.90989,0.82134,0.55638,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3720,ERR1397070,ERX1468329,ERS1023476,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 15,SAMEA3716327,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716327|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:36Z|INSDC status:public|Submitter Id:22c884f0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TCAGGAGG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22c884f0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#39,15566379,Illumina sequencing of library 15566379  constructed from sample accession ERS1023476 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCAGGAGG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#39.cram,cram,651681420.0,5012934.0,SC RUN 18715 8#39,0:55 1:75,A:177714223;C:117422574;G:117918287;T:238624462;N:1874,55,75,,,177714223,117422574,117918287,238624462,1874,ERX1468329,ERS1023476,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.30203,0.82354,0.13321,0.08477,0.97595,0.91587,0.83389,0.66905,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3721,ERR1397069,ERX1468328,ERS1023475,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 14,SAMEA3716326,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716326|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:36Z|INSDC status:public|Submitter Id:22bf8440 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TCTCACGG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22bf8440 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#38,15566378,Illumina sequencing of library 15566378  constructed from sample accession ERS1023475 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCTCACGG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#38.cram,cram,662342720.0,5094944.0,SC RUN 18715 8#38,0:55 1:75,A:178580497;C:115417983;G:120453380;T:247888799;N:2061,55,75,,,178580497,115417983,120453380,247888799,2061,ERX1468328,ERS1023475,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.30376,0.86314,0.16369,0.09566,0.97739,0.91557,0.82256,0.68053,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3722,ERR1397068,ERX1468327,ERS1023474,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 13,SAMEA3716325,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716325|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:35Z|INSDC status:public|Submitter Id:22b68390 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf tert wild type sibling adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The wild type adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter wild type fish from the clutch. A 8 base indexing sequence TACTTCGG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22b68390 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#37,15566377,Illumina sequencing of library 15566377  constructed from sample accession ERS1023474 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TACTTCGG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#37.cram,cram,696072520.0,5354404.0,SC RUN 18715 8#37,0:55 1:75,A:186700497;C:125763963;G:128699401;T:254906770;N:1889,55,75,,,186700497,125763963,128699401,254906770,1889,ERX1468327,ERS1023474,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.27617,0.85667,0.1301,0.08773,0.97684,0.91514,0.79798,0.64081,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3723,ERR1397067,ERX1468326,ERS1023473,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 12,SAMEA3716324,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716324|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:35Z|INSDC status:public|Submitter Id:22ad82e0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TGAACTGG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22ad82e0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#36,15566376,Illumina sequencing of library 15566376  constructed from sample accession ERS1023473 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGAACTGG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#36.cram,cram,731306420.0,5625434.0,SC RUN 18715 8#36,0:55 1:75,A:193958125;C:134273924;G:136394750;T:266677546;N:2075,55,75,,,193958125,134273924,136394750,266677546,2075,ERX1468326,ERS1023473,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.24621,0.84195,0.08689,0.07535,0.97638,0.91851,0.80524,0.61404,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3724,ERR1397066,ERX1468325,ERS1023472,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 11,SAMEA3716323,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716323|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:34Z|INSDC status:public|Submitter Id:22a45b20 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TTGGTATG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22a45b20 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#35,15566375,Illumina sequencing of library 15566375  constructed from sample accession ERS1023472 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTGGTATG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#35.cram,cram,665997670.0,5123059.0,SC RUN 18715 8#35,0:55 1:75,A:181566480;C:120809592;G:121644240;T:241975360;N:1998,55,75,,,181566480,120809592,121644240,241975360,1998,ERX1468325,ERS1023472,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.26496,0.82274,0.10057,0.07626,0.97441,0.91595,0.81657,0.64747,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3725,ERR1397065,ERX1468324,ERS1023471,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 10,SAMEA3716322,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716322|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:34Z|INSDC status:public|Submitter Id:229b8180 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TAACGCTG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:229b8180 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#34,15566374,Illumina sequencing of library 15566374  constructed from sample accession ERS1023471 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TAACGCTG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#34.cram,cram,694634590.0,5343343.0,SC RUN 18715 8#34,0:55 1:75,A:186244179;C:125715272;G:128699352;T:253973213;N:2574,55,75,,,186244179,125715272,128699352,253973213,2574,ERX1468324,ERS1023471,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.26311,0.82812,0.10775,0.09376,0.97313,0.90983,0.80442,0.5326,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3726,ERR1397064,ERX1468323,ERS1023470,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 9,SAMEA3716321,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716321|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:33Z|INSDC status:public|Submitter Id:228fe8c0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TCGAAGTG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:228fe8c0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#33,15566373,Illumina sequencing of library 15566373  constructed from sample accession ERS1023470 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCGAAGTG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#33.cram,cram,694711290.0,5343933.0,SC RUN 18715 8#33,0:55 1:75,A:188079963;C:126858196;G:128818574;T:250952301;N:2256,55,75,,,188079963,126858196,128818574,250952301,2256,ERX1468323,ERS1023470,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.25746,0.83173,0.07713,0.08005,0.97437,0.91431,0.83094,0.63526,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3727,ERR1397063,ERX1468322,ERS1023469,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 8,SAMEA3716320,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716320|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:33Z|INSDC status:public|Submitter Id:2286e810 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TTCCATTG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:2286e810 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#32,15566372,Illumina sequencing of library 15566372  constructed from sample accession ERS1023469 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTCCATTG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#32.cram,cram,655146440.0,5039588.0,SC RUN 18715 8#32,0:55 1:75,A:178645019;C:116883757;G:120893841;T:238720622;N:3201,55,75,,,178645019,116883757,120893841,238720622,3201,ERX1468322,ERS1023469,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.24055,0.83244,0.1125,0.10225,0.97291,0.90928,0.77504,0.6656,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3728,ERR1397062,ERX1468321,ERS1023468,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 7,SAMEA3716319,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716319|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:32Z|INSDC status:public|Submitter Id:227caee0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TAGTCTTG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:227caee0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#31,15566371,Illumina sequencing of library 15566371  constructed from sample accession ERS1023468 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TAGTCTTG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#31.cram,cram,730891590.0,5622243.0,SC RUN 18715 8#31,0:55 1:75,A:198514053;C:130409691;G:134606848;T:267358664;N:2334,55,75,,,198514053,130409691,134606848,267358664,2334,ERX1468321,ERS1023468,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28597,0.85275,0.12917,0.11083,0.97504,0.9165,0.79987,0.6128,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3729,ERR1397061,ERX1468320,ERS1023467,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 6,SAMEA3716318,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716318|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:32Z|INSDC status:public|Submitter Id:2271b260 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TGTGGTTG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:2271b260 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#30,15566370,Illumina sequencing of library 15566370  constructed from sample accession ERS1023467 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGTGGTTG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#30.cram,cram,660668580.0,5082066.0,SC RUN 18715 8#30,0:55 1:75,A:179797750;C:120562968;G:120663983;T:239642031;N:1848,55,75,,,179797750,120562968,120663983,239642031,1848,ERX1468320,ERS1023467,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.24637,0.83519,0.11648,0.10047,0.97112,0.90806,0.75586,0.63304,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3730,ERR1397060,ERX1468319,ERS1023466,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 5,SAMEA3716317,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716317|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:31Z|INSDC status:public|Submitter Id:2266b5e0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TCCTCAAT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:2266b5e0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#29,15566369,Illumina sequencing of library 15566369  constructed from sample accession ERS1023466 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCCTCAAT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#29.cram,cram,641844320.0,4937264.0,SC RUN 18715 8#29,0:55 1:75,A:170784947;C:114236819;G:119506285;T:237313307;N:2962,55,75,,,170784947,114236819,119506285,237313307,2962,ERX1468319,ERS1023466,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.24614,0.83823,0.11448,0.08337,0.97356,0.90593,0.76773,0.64034,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3731,ERR1397059,ERX1468318,ERS1023465,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 4,SAMEA3716316,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716316|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:31Z|INSDC status:public|Submitter Id:225be070 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TACAGGAT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:225be070 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#28,15566368,Illumina sequencing of library 15566368  constructed from sample accession ERS1023465 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TACAGGAT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#28.cram,cram,587079870.0,4515999.0,SC RUN 18715 8#28,,,,,,,,,,,,ERX1468318,ERS1023465,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.14143,0.59868,0.09232,0.09907,0.97648,0.91616,0.55833,0.54275,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3732,ERR1397058,ERX1468317,ERS1023464,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 3,SAMEA3716315,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716315|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:30Z|INSDC status:public|Submitter Id:22506ec0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TAGTGACT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22506ec0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#27,15566367,Illumina sequencing of library 15566367  constructed from sample accession ERS1023464 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TAGTGACT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#27.cram,cram,656951230.0,5053471.0,SC RUN 18715 8#27,0:55 1:75,A:176154435;C:112733310;G:125269118;T:242792191;N:2176,55,75,,,176154435,112733310,125269118,242792191,2176,ERX1468317,ERS1023464,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.30886,0.85046,0.11844,0.0971,0.97658,0.91583,0.89951,0.76866,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3733,ERR1397057,ERX1468316,ERS1023463,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 2,SAMEA3716314,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716314|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:30Z|INSDC status:public|Submitter Id:22452420 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TTCCTGCT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:22452420 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#26,15566366,Illumina sequencing of library 15566366  constructed from sample accession ERS1023463 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTCCTGCT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#26.cram,cram,637042380.0,4900326.0,SC RUN 18715 8#26,0:55 1:75,A:169999761;C:113778382;G:122043906;T:231217073;N:3258,55,75,,,169999761,113778382,122043906,231217073,3258,ERX1468316,ERS1023463,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.31489,0.85017,0.11073,0.08344,0.97366,0.91528,0.83569,0.68063,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3734,ERR1397056,ERX1468315,ERS1023462,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 110 1 1,SAMEA3716313,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716313|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:29Z|INSDC status:public|Submitter Id:2239d980 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 month and 1 wpf homozygous tert adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. The homozygous adults were obtained by crossing parents heterozygous for tert  sa6541 and growing the quarter homozygous fish from the clutch. A 8 base indexing sequence TGCGATCT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph110|sample name:2239d980 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#25,15566365,Illumina sequencing of library 15566365  constructed from sample accession ERS1023462 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGCGATCT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#25.cram,cram,567513050.0,4365485.0,SC RUN 18715 8#25,0:55 1:75,A:150769806;C:98997671;G:107141083;T:210602580;N:1910,55,75,,,150769806,98997671,107141083,210602580,1910,ERX1468315,ERS1023462,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.31513,0.8639,0.11016,0.07449,0.9752,0.90985,0.88328,0.70792,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3735,ERR1397055,ERX1468314,ERS1023461,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 12,SAMEA3716312,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716312|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:29Z|INSDC status:public|Submitter Id:22303c90 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TTGACTCT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:22303c90 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#24,15566364,Illumina sequencing of library 15566364  constructed from sample accession ERS1023461 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTGACTCT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#24.cram,cram,541389290.0,4164533.0,SC RUN 18715 8#24,0:55 1:75,A:149630682;C:93128764;G:102059718;T:196567547;N:2579,55,75,,,149630682,93128764,102059718,196567547,2579,ERX1468314,ERS1023461,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.31938,0.80432,0.16112,0.16984,0.97183,0.90893,0.82211,0.73255,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3736,ERR1397054,ERX1468313,ERS1023460,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 11,SAMEA3716311,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716311|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:28Z|INSDC status:public|Submitter Id:22265180 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TGCATAGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:22265180 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#23,15566363,Illumina sequencing of library 15566363  constructed from sample accession ERS1023460 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGCATAGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#23.cram,cram,571818000.0,4398600.0,SC RUN 18715 8#23,0:55 1:75,A:158875843;C:99417127;G:106799809;T:206723059;N:2162,55,75,,,158875843,99417127,106799809,206723059,2162,ERX1468313,ERS1023460,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.30236,0.79208,0.15953,0.16714,0.9693,0.90343,0.81603,0.53838,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3737,ERR1397053,ERX1468312,ERS1023459,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 10,SAMEA3716310,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716310|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:28Z|INSDC status:public|Submitter Id:221bf140 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TGATACGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:221bf140 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#22,15566362,Illumina sequencing of library 15566362  constructed from sample accession ERS1023459 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGATACGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#22.cram,cram,486876910.0,3745207.0,SC RUN 18715 8#22,0:55 1:75,A:129941384;C:87127302;G:90702777;T:179103590;N:1857,55,75,,,129941384,87127302,90702777,179103590,1857,ERX1468312,ERS1023459,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.27884,0.83381,0.12097,0.11714,0.97039,0.8983,0.81908,0.64542,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3738,ERR1397052,ERX1468311,ERS1023458,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 9,SAMEA3716309,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716309|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:27Z|INSDC status:public|Submitter Id:22119100 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TCGAGCGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:22119100 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#21,15566361,Illumina sequencing of library 15566361  constructed from sample accession ERS1023458 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCGAGCGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#21.cram,cram,321968660.0,2476682.0,SC RUN 18715 8#21,0:55 1:75,A:85611967;C:56953379;G:60212796;T:119189501;N:1017,55,75,,,85611967,56953379,60212796,119189501,1017,ERX1468311,ERS1023458,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.2616,0.85744,0.09546,0.10386,0.97341,0.89327,0.84115,0.52093,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3739,ERR1397051,ERX1468310,ERS1023457,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 8,SAMEA3716308,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716308|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:27Z|INSDC status:public|Submitter Id:220730c0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TTGGAGGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:220730c0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#20,15566360,Illumina sequencing of library 15566360  constructed from sample accession ERS1023457 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTGGAGGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#20.cram,cram,472624360.0,3635572.0,SC RUN 18715 8#20,0:55 1:75,A:126831392;C:83556709;G:88455943;T:173779093;N:1223,55,75,,,126831392,83556709,88455943,173779093,1223,ERX1468310,ERS1023457,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.30388,0.84241,0.11508,0.11573,0.97116,0.90003,0.39774,0.70203,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3740,ERR1397050,ERX1468309,ERS1023456,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 7,SAMEA3716307,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716307|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:26Z|INSDC status:public|Submitter Id:21fc5b50 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TCTGCTGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:21fc5b50 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#19,15566359,Illumina sequencing of library 15566359  constructed from sample accession ERS1023456 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCTGCTGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#19.cram,cram,602614090.0,4635493.0,SC RUN 18715 8#19,0:55 1:75,A:163499230;C:104602930;G:113213757;T:221296100;N:2073,55,75,,,163499230,104602930,113213757,221296100,2073,ERX1468309,ERS1023456,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.35019,0.84609,0.13232,0.13362,0.96822,0.89412,0.8825,0.74339,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3741,ERR1397049,ERX1468308,ERS1023455,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 6,SAMEA3716306,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716306|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:26Z|INSDC status:public|Submitter Id:21f22220 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TTCTGTGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:21f22220 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#18,15566358,Illumina sequencing of library 15566358  constructed from sample accession ERS1023455 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTCTGTGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#18.cram,cram,620771320.0,4775164.0,SC RUN 18715 8#18,0:55 1:75,A:170120424;C:109984137;G:117603882;T:223060767;N:2110,55,75,,,170120424,109984137,117603882,223060767,2110,ERX1468308,ERS1023455,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.29675,0.83916,0.12868,0.14148,0.96613,0.8984,0.79183,0.65344,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3742,ERR1397048,ERX1468307,ERS1023454,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 5,SAMEA3716305,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716305|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:25Z|INSDC status:public|Submitter Id:21e81000 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TGTACCTT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:21e81000 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#17,15566357,Illumina sequencing of library 15566357  constructed from sample accession ERS1023454 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGTACCTT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#17.cram,cram,620956960.0,4776592.0,SC RUN 18715 8#17,0:55 1:75,A:167465723;C:108421495;G:117858045;T:227208072;N:3625,55,75,,,167465723,108421495,117858045,227208072,3625,ERX1468307,ERS1023454,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28111,0.84477,0.11758,0.12272,0.97137,0.89832,0.849,0.70528,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3743,ERR1397047,ERX1468306,ERS1023453,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 4,SAMEA3716304,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716304|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:25Z|INSDC status:public|Submitter Id:21dc9e50 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TCCGTCTT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:21dc9e50 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#16,15566356,Illumina sequencing of library 15566356  constructed from sample accession ERS1023453 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCCGTCTT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#16.cram,cram,647011300.0,4977010.0,SC RUN 18715 8#16,0:55 1:75,A:170845029;C:117050187;G:126808386;T:232304633;N:3065,55,75,,,170845029,117050187,126808386,232304633,3065,ERX1468306,ERS1023453,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.26816,0.85786,0.09644,0.09888,0.9694,0.90751,0.79908,0.6443,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3744,ERR1397046,ERX1468305,ERS1023452,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 3,SAMEA3716303,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716303|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:24Z|INSDC status:public|Submitter Id:21d34f80 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TAAGCGTT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:21d34f80 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#15,15566355,Illumina sequencing of library 15566355  constructed from sample accession ERS1023452 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TAAGCGTT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#15.cram,cram,457286700.0,3517590.0,SC RUN 18715 8#15,0:55 1:75,A:118667898;C:84631347;G:85673386;T:168312768;N:1301,55,75,,,118667898,84631347,85673386,168312768,1301,ERX1468305,ERS1023452,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.23276,0.86209,0.10575,0.09847,0.97358,0.9052,0.78944,0.62306,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3745,ERR1397045,ERX1468304,ERS1023451,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 2,SAMEA3716302,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716302|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:24Z|INSDC status:public|Submitter Id:21c8a120 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TCTCGGTT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:21c8a120 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#14,15566354,Illumina sequencing of library 15566354  constructed from sample accession ERS1023451 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TCTCGGTT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#14.cram,cram,653102060.0,5023862.0,SC RUN 18715 8#14,0:55 1:75,A:174751774;C:115388239;G:124463430;T:238496439;N:2178,55,75,,,174751774,115388239,124463430,238496439,2178,ERX1468304,ERS1023451,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.2967,0.86134,0.12119,0.11716,0.97072,0.9011,0.84585,0.56695,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3746,ERR1397044,ERX1468303,ERS1023450,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 113 1 1,SAMEA3716301,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   26 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716301|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:23Z|INSDC status:public|Submitter Id:21beb610 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 26 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TGGTTGTT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph113|sample name:21beb610 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#13,15566353,Illumina sequencing of library 15566353  constructed from sample accession ERS1023450 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGGTTGTT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#13.cram,cram,551198960.0,4239992.0,SC RUN 18715 8#13,0:55 1:75,A:148509015;C:97254852;G:104086449;T:201346621;N:2023,55,75,,,148509015,97254852,104086449,201346621,2023,ERX1468303,ERS1023450,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28017,0.87069,0.11381,0.11448,0.97133,0.90106,0.84612,0.68133,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3747,ERR1397043,ERX1468302,ERS1023449,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 12,SAMEA3716300,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716300|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:23Z|INSDC status:public|Submitter Id:21b4cb00 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence CTTGTACT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:21b4cb00 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#12,15566352,Illumina sequencing of library 15566352  constructed from sample accession ERS1023449 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence CTTGTACT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#12.cram,cram,683965360.0,5261272.0,SC RUN 18715 8#12,0:55 1:75,A:181490420;C:124392573;G:129140040;T:248939248;N:3079,55,75,,,181490420,124392573,129140040,248939248,3079,ERX1468302,ERS1023449,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.25401,0.85131,0.10056,0.08236,0.97431,0.91419,0.81852,0.53153,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3748,ERR1397042,ERX1468301,ERS1023448,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 11,SAMEA3716299,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716299|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:22Z|INSDC status:public|Submitter Id:21a98060 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence GGCTACAG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:21a98060 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#11,15566351,Illumina sequencing of library 15566351  constructed from sample accession ERS1023448 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence GGCTACAG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#11.cram,cram,678711150.0,5220855.0,SC RUN 18715 8#11,0:55 1:75,A:179239904;C:124839850;G:129344689;T:245284425;N:2282,55,75,,,179239904,124839850,129344689,245284425,2282,ERX1468301,ERS1023448,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.25995,0.85813,0.08951,0.08509,0.97362,0.91532,0.84938,0.5878,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3749,ERR1397041,ERX1468300,ERS1023447,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 10,SAMEA3716298,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716298|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:22Z|INSDC status:public|Submitter Id:219e5cd0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TAGCTTGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:219e5cd0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#10,15566350,Illumina sequencing of library 15566350  constructed from sample accession ERS1023447 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TAGCTTGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#10.cram,cram,512021380.0,3938626.0,SC RUN 18715 8#10,0:55 1:75,A:134633141;C:95267180;G:96292177;T:185827489;N:1393,55,75,,,134633141,95267180,96292177,185827489,1393,ERX1468300,ERS1023447,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.27511,0.8473,0.08789,0.07504,0.97484,0.91932,0.83725,0.53104,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3750,ERR1397040,ERX1468299,ERS1023446,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 9,SAMEA3716297,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716297|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:21Z|INSDC status:public|Submitter Id:219423a0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence GATCAGCG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:219423a0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#9,15566349,Illumina sequencing of library 15566349  constructed from sample accession ERS1023446 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence GATCAGCG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#9.cram,cram,606955700.0,4668890.0,SC RUN 18715 8#9,0:55 1:75,A:161328190;C:111573232;G:113967253;T:220085334;N:1691,55,75,,,161328190,111573232,113967253,220085334,1691,ERX1468299,ERS1023446,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.27581,0.81211,0.10852,0.0992,0.97311,0.91023,0.84113,0.65956,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3751,ERR1397039,ERX1468298,ERS1023445,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 8,SAMEA3716296,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716296|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:21Z|INSDC status:public|Submitter Id:218a3890 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence ACTTGATG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:218a3890 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#8,15566348,Illumina sequencing of library 15566348  constructed from sample accession ERS1023445 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence ACTTGATG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#8.cram,cram,631737210.0,4859517.0,SC RUN 18715 8#8,0:55 1:75,A:168090778;C:115132937;G:119109490;T:229402219;N:1786,55,75,,,168090778,115132937,119109490,229402219,1786,ERX1468298,ERS1023445,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28735,0.86714,0.09468,0.08742,0.97266,0.91165,0.82804,0.43981,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3752,ERR1397038,ERX1468297,ERS1023444,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 7,SAMEA3716295,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716295|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:20Z|INSDC status:public|Submitter Id:217fd850 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence CAGATCTG is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:217fd850 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#7,15566347,Illumina sequencing of library 15566347  constructed from sample accession ERS1023444 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence CAGATCTG.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#7.cram,cram,629907200.0,4845440.0,SC RUN 18715 8#7,0:55 1:75,A:167238249;C:113933791;G:119656035;T:229077206;N:1919,55,75,,,167238249,113933791,119656035,229077206,1919,ERX1468297,ERS1023444,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.28015,0.85121,0.09694,0.0866,0.97579,0.91873,0.49517,0.67663,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3753,ERR1397037,ERX1468296,ERS1023443,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 6,SAMEA3716294,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716294|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:20Z|INSDC status:public|Submitter Id:21757810 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence GCCAATGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:21757810 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#6,15566346,Illumina sequencing of library 15566346  constructed from sample accession ERS1023443 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence GCCAATGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#6.cram,cram,549775980.0,4229046.0,SC RUN 18715 8#6,0:55 1:75,A:145007963;C:100751331;G:102099529;T:201915350;N:1807,55,75,,,145007963,100751331,102099529,201915350,1807,ERX1468296,ERS1023443,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.2874,0.85663,0.08661,0.0687,0.97788,0.91705,0.84995,0.44346,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3754,ERR1397036,ERX1468295,ERS1023442,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 5,SAMEA3716293,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716293|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:19Z|INSDC status:public|Submitter Id:216b17d0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence ACAGTGGT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:216b17d0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#5,15566345,Illumina sequencing of library 15566345  constructed from sample accession ERS1023442 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence ACAGTGGT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#5.cram,cram,609585470.0,4689119.0,SC RUN 18715 8#5,0:55 1:75,A:160001548;C:112004296;G:114635311;T:222942641;N:1674,55,75,,,160001548,112004296,114635311,222942641,1674,ERX1468295,ERS1023442,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.24791,0.87348,0.09277,0.0825,0.97536,0.91543,0.83597,0.65901,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3755,ERR1397035,ERX1468294,ERS1023441,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 4,SAMEA3716292,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716292|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:19Z|INSDC status:public|Submitter Id:2160b790 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TGACCACT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:2160b790 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#4,15566344,Illumina sequencing of library 15566344  constructed from sample accession ERS1023441 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TGACCACT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#4.cram,cram,678764840.0,5221268.0,SC RUN 18715 8#4,0:55 1:75,A:178838072;C:124911287;G:130189123;T:244823100;N:3258,55,75,,,178838072,124911287,130189123,244823100,3258,ERX1468294,ERS1023441,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.25304,0.81978,0.09241,0.08656,0.97676,0.91747,0.84034,0.65122,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3756,ERR1397034,ERX1468293,ERS1023440,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 3,SAMEA3716291,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716291|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:18Z|INSDC status:public|Submitter Id:21556cf0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence TTAGGCAT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:21556cf0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#3,15566343,Illumina sequencing of library 15566343  constructed from sample accession ERS1023440 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence TTAGGCAT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#3.cram,cram,681501470.0,5242319.0,SC RUN 18715 8#3,0:55 1:75,A:181660061;C:124276603;G:127126649;T:248435294;N:2863,55,75,,,181660061,124276603,127126649,248435294,2863,ERX1468293,ERS1023440,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.27754,0.81927,0.08779,0.07376,0.97717,0.92334,0.85395,0.62252,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3757,ERR1397033,ERX1468292,ERS1023439,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 2,SAMEA3716290,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716290|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:18Z|INSDC status:public|Submitter Id:21498610 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence CGATGTTT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:21498610 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#2,15566342,Illumina sequencing of library 15566342  constructed from sample accession ERS1023439 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence CGATGTTT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#2.cram,cram,696821970.0,5360169.0,SC RUN 18715 8#2,0:55 1:75,A:186653953;C:128161902;G:131712165;T:250291266;N:2684,55,75,,,186653953,128161902,131712165,250291266,2684,ERX1468292,ERS1023439,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.24475,0.81186,0.08713,0.06706,0.97798,0.92281,0.83555,0.55607,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
3758,ERR1397032,ERX1468291,ERS1023438,ERP013838,PRJEB12367,Transcriptome profiling of zebrafish muscle from wild type and tert knockout adults,Transcriptome_profiling_of_zebrafish_muscle_from_wild_type_and_tert_knockout_adults-sc-4036,Transcriptome Analysis,Paired end sequence data from the IlluminaHiSeq was prepared from RNA of dissected muscle from wildtype and tert homozygous adult zebrafish for transcriptional profiling,ArrayExpress:E ERAD 456,,,ZMP phentoype 112 1 1,SAMEA3716289,Wellcome Sanger Institute,ArrayExpress DevelopmentalStage:Adult   ZFS:0000044   3 month|ArrayExpress OrganismPart:Adult muscle|ArrayExpress Species:Danio rerio|ENA first public:2016 05 03|ENA last update:2015 12 17|External Id:SAMEA3716289|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2016 05 03T14:23:28Z|INSDC last update:2015 12 17T16:32:17Z|INSDC status:public|Submitter Id:2134eca0 a400 11e5 8adb 68b59976a382|common name:zebrafish|sample description:3 prime end enriched mRNA from a 3 mpf wild type adult zebrafish muscle tissue dissected from the region between the anal fin and the caudal fin plus ERCC spike mix 2 Ambion. A 8 base indexing sequence ATCACGTT is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi bin/Projects/D rerio/zmp/search.pl?q=zmp ph112|sample name:2134eca0 a400 11e5 8adb 68b59976a382|strain:mixed,,,,,,,,,Illumina HiSeq 2000 paired end sequencing,SC EXP 18715 8#1,15566341,Illumina sequencing of library 15566341  constructed from sample accession ERS1023438 for study accession ERP013838.  This is part of an Illumina multiplexed sequencing run 18715 8.  This submission includes reads tagged with the sequence ATCACGTT.,Transcriptome counting qPCR only,,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 2000,,ERP013838,Illumina HiSeq 2000 paired end sequencing,ENA FIRST PUBLIC:2016 05 03|ENA LAST UPDATE:2018 11 16,18715_8#1.cram,cram,637072930.0,4900561.0,SC RUN 18715 8#1,0:55 1:75,A:169596079;C:116706958;G:122297212;T:228469365;N:3316,55,75,,,169596079,116706958,122297212,228469365,3316,ERX1468291,ERS1023438,ERA612387,European Nucleotide Archive,Wellcome Sanger Institute,2,0.2369,0.84014,0.08404,0.07279,0.9764,0.92289,0.81134,0.63042,55,75,T,B,mate1 technical by mapping diff,illumina,hiseq_era,3prime,cdna_unspecified,unknown,bulk,unknown,unknown,,United Kingdom,2015-12-17,Adult,Adult,Multi-tissue,Multi-system
5883,ERR1889880,ERX1950378,ERS1612644,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H9,SAMEA103923476,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923476|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H9|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:55393|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H9|scientific name:Danio rerio|side scatter:152|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H9 p,WT P5 H9 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N726_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N726_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,454422000.0,1817688.0,E MTAB 5530:SLX 10882.N726 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:136748884;C:93946720;G:85071329;T:138648880;N:6187,125,125,,,136748884,93946720,85071329,138648880,6187,ERX1950378,ERS1612644,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.85479,0.85524,0.30799,0.31054,0.96152,0.96185,0.57277,0.5503,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5884,ERR1889879,ERX1950377,ERS1612643,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H8,SAMEA103923475,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923475|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H8|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:51437|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H8|scientific name:Danio rerio|side scatter:87|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H8 p,WT P5 H8 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N724_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N724_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,186247500.0,744990.0,E MTAB 5530:SLX 10882.N724 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:50686706;C:43414828;G:40798159;T:51345541;N:2266,125,125,,,50686706,43414828,40798159,51345541,2266,ERX1950377,ERS1612643,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.88281,0.8845,0.1433,0.14573,0.96499,0.96593,0.54281,0.53863,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5885,ERR1889878,ERX1950376,ERS1612642,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H7,SAMEA103923474,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923474|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H7|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:47964|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H7|scientific name:Danio rerio|side scatter:39|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H7 p,WT P5 H7 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N723_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N723_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,348410500.0,1393642.0,E MTAB 5530:SLX 10882.N723 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:96686032;C:79087034;G:73210700;T:99422219;N:4515,125,125,,,96686032,79087034,73210700,99422219,4515,ERX1950376,ERS1612642,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.7196,0.72147,0.37859,0.38193,0.9833,0.98364,0.55194,0.559,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5886,ERR1889877,ERX1950375,ERS1612641,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H6,SAMEA103923473,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923473|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H6|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:48357|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H6|scientific name:Danio rerio|side scatter:131|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H6 p,WT P5 H6 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N722_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N722_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,181295500.0,725182.0,E MTAB 5530:SLX 10882.N722 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:50876125;C:40695824;G:38118373;T:51602428;N:2750,125,125,,,50876125,40695824,38118373,51602428,2750,ERX1950375,ERS1612641,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.87224,0.87337,0.19601,0.19886,0.94763,0.9485,0.57303,0.57966,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5887,ERR1889876,ERX1950374,ERS1612640,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H5,SAMEA103923472,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923472|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H5|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:53127|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H5|scientific name:Danio rerio|side scatter:132|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H5 p,WT P5 H5 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N721_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N721_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,415522750.0,1662091.0,E MTAB 5530:SLX 10882.N721 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:110568576;C:99503791;G:92964646;T:112479694;N:6043,125,125,,,110568576,99503791,92964646,112479694,6043,ERX1950374,ERS1612640,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.84994,0.85395,0.20988,0.21306,0.99135,0.99176,0.49657,0.49463,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5888,ERR1889875,ERX1950373,ERS1612639,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H4,SAMEA103923471,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923471|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H4|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:47393|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H4|scientific name:Danio rerio|side scatter:91|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H4 p,WT P5 H4 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N720_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N720_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,248216250.0,992865.0,E MTAB 5530:SLX 10882.N720 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:69927538;C:55390480;G:51305326;T:71589640;N:3266,125,125,,,69927538,55390480,51305326,71589640,3266,ERX1950373,ERS1612639,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.80077,0.80418,0.26935,0.27239,0.964,0.96416,0.56825,0.56826,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5889,ERR1889874,ERX1950372,ERS1612638,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H3,SAMEA103923470,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923470|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H3|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:52142|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H3|scientific name:Danio rerio|side scatter:100|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H3 p,WT P5 H3 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N719_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N719_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,207711000.0,830844.0,E MTAB 5530:SLX 10882.N719 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:59018212;C:45911934;G:42512420;T:60265533;N:2901,125,125,,,59018212,45911934,42512420,60265533,2901,ERX1950372,ERS1612638,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.75165,0.75465,0.22896,0.23339,0.95698,0.95785,0.56434,0.56661,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5890,ERR1889873,ERX1950371,ERS1612637,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H2,SAMEA103923469,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923469|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H2|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:56058|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H2|scientific name:Danio rerio|side scatter:91|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H2 p,WT P5 H2 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N718_S522.C9FTNANXX.s_7.r_2.fq.gz SLX-10882.N718_S522.C9FTNANXX.s_7.r_1.fq.gz,fastq fastq,146531000.0,586124.0,E MTAB 5530:SLX 10882.N718 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:40755958;C:33204212;G:30945794;T:41623422;N:1614,125,125,,,40755958,33204212,30945794,41623422,1614,ERX1950371,ERS1612637,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.86008,0.86341,0.21708,0.21977,0.95217,0.95256,0.59026,0.57925,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5891,ERR1889872,ERX1950370,ERS1612636,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H12,SAMEA103923468,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923468|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H12|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:49880|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H12|scientific name:Danio rerio|side scatter:139|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H12 p,WT P5 H12 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N729_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N729_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,161489250.0,645957.0,E MTAB 5530:SLX 10882.N729 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:42374075;C:39368051;G:36681758;T:43063658;N:1708,125,125,,,42374075,39368051,36681758,43063658,1708,ERX1950370,ERS1612636,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.83679,0.84039,0.10201,0.10477,0.9609,0.96219,0.49851,0.50388,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5892,ERR1889871,ERX1950369,ERS1612635,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H11,SAMEA103923467,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923467|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H11|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:48487|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H11|scientific name:Danio rerio|side scatter:89|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H11 p,WT P5 H11 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N728_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N728_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,158863250.0,635453.0,E MTAB 5530:SLX 10882.N728 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:44585477;C:35694997;G:33000169;T:45580356;N:2251,125,125,,,44585477,35694997,33000169,45580356,2251,ERX1950369,ERS1612635,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.82793,0.83103,0.25297,0.25621,0.96396,0.96499,0.56852,0.57747,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5893,ERR1889870,ERX1950368,ERS1612634,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H10,SAMEA103923466,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:52Z|External Id:SAMEA103923466|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:52Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H10|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:49240|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H10|scientific name:Danio rerio|side scatter:103|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H10 p,WT P5 H10 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N727_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N727_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,348551500.0,1394206.0,E MTAB 5530:SLX 10882.N727 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:101118670;C:75215300;G:68945291;T:103267609;N:4630,125,125,,,101118670,75215300,68945291,103267609,4630,ERX1950368,ERS1612634,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.84815,0.85102,0.30595,0.30969,0.96682,0.96743,0.51714,0.52278,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5894,ERR1889869,ERX1950367,ERS1612633,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 H1,SAMEA103923465,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923465|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 H1|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:57826|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 H1|scientific name:Danio rerio|side scatter:130|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 H1 p,WT P5 H1 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N716_S522.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N716_S522.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,270394750.0,1081579.0,E MTAB 5530:SLX 10882.N716 S522.C9FTNANXX.s 7.r ,0:125 1:125,A:73655517;C:63136790;G:58258231;T:75340574;N:3638,125,125,,,73655517,63136790,58258231,75340574,3638,ERX1950367,ERS1612633,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.49844,0.49445,0.19246,0.19232,0.9724,0.97266,0.56544,0.56022,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5895,ERR1889868,ERX1950366,ERS1612632,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G9,SAMEA103923464,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923464|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G9|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:49332|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G9|scientific name:Danio rerio|side scatter:121|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G9 p,WT P5 G9 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N726_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N726_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,247298500.0,989194.0,E MTAB 5530:SLX 10882.N726 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:71103309;C:54308331;G:49718102;T:72165293;N:3465,125,125,,,71103309,54308331,49718102,72165293,3465,ERX1950366,ERS1612632,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.846,0.84611,0.30413,0.30786,0.96552,0.96641,0.59113,0.60044,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5896,ERR1889867,ERX1950365,ERS1612631,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G8,SAMEA103923463,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923463|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G8|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:47325|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G8|scientific name:Danio rerio|side scatter:58|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G8 p,WT P5 G8 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N724_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N724_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,152795250.0,611181.0,E MTAB 5530:SLX 10882.N724 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:42420452;C:34645333;G:32604949;T:43122474;N:2042,125,125,,,42420452,34645333,32604949,43122474,2042,ERX1950365,ERS1612631,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.87026,0.87123,0.21882,0.22166,0.95217,0.95331,0.54351,0.558,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5897,ERR1889866,ERX1950364,ERS1612630,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G7,SAMEA103923462,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923462|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G7|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:54259|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G7|scientific name:Danio rerio|side scatter:131|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G7 p,WT P5 G7 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N723_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N723_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,161275500.0,645102.0,E MTAB 5530:SLX 10882.N723 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:44615941;C:36684169;G:34349960;T:45623066;N:2364,125,125,,,44615941,36684169,34349960,45623066,2364,ERX1950364,ERS1612630,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.76098,0.76326,0.20601,0.20863,0.95416,0.95469,0.55476,0.55633,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5898,ERR1889865,ERX1950363,ERS1612629,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G6,SAMEA103923461,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923461|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G6|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:57696|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G6|scientific name:Danio rerio|side scatter:109|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G6 p,WT P5 G6 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N722_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N722_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,169127000.0,676508.0,E MTAB 5530:SLX 10882.N722 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:46598538;C:38702157;G:36369879;T:47454343;N:2083,125,125,,,46598538,38702157,36369879,47454343,2083,ERX1950363,ERS1612629,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.86647,0.8688,0.26961,0.27259,0.97035,0.97055,0.59022,0.58951,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5899,ERR1889864,ERX1950362,ERS1612628,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G5,SAMEA103923460,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923460|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G5|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:49825|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G5|scientific name:Danio rerio|side scatter:96|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G5 p,WT P5 G5 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N721_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N721_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,160674500.0,642698.0,E MTAB 5530:SLX 10882.N721 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:42242295;C:38885395;G:36715695;T:42829381;N:1734,125,125,,,42242295,38885395,36715695,42829381,1734,ERX1950362,ERS1612628,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.88993,0.89165,0.11626,0.11825,0.96607,0.96708,0.48505,0.48004,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5900,ERR1889863,ERX1950361,ERS1612627,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G4,SAMEA103923459,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923459|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G4|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:59595|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G4|scientific name:Danio rerio|side scatter:128|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G4 p,WT P5 G4 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N720_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N720_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,215294250.0,861177.0,E MTAB 5530:SLX 10882.N720 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:58820107;C:49745945;G:46718802;T:60006855;N:2541,125,125,,,58820107,49745945,46718802,60006855,2541,ERX1950361,ERS1612627,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.71909,0.71863,0.28839,0.29147,0.97475,0.975,0.53757,0.5459,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5901,ERR1889862,ERX1950360,ERS1612626,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G3,SAMEA103923458,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923458|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G3|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:52146|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G3|scientific name:Danio rerio|side scatter:109|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G3 p,WT P5 G3 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N719_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N719_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,169196500.0,676786.0,E MTAB 5530:SLX 10882.N719 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:46905975;C:38476120;G:35996313;T:47815682;N:2410,125,125,,,46905975,38476120,35996313,47815682,2410,ERX1950360,ERS1612626,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.80664,0.80791,0.29012,0.29478,0.97364,0.97382,0.45404,0.4625,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5902,ERR1889861,ERX1950359,ERS1612625,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G2,SAMEA103923457,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923457|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G2|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:47724|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G2|scientific name:Danio rerio|side scatter:81|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G2 p,WT P5 G2 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N718_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N718_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,162005250.0,648021.0,E MTAB 5530:SLX 10882.N718 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:42407136;C:39227455;G:37245549;T:43123434;N:1676,125,125,,,42407136,39227455,37245549,43123434,1676,ERX1950359,ERS1612625,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.80989,0.81143,0.12934,0.13121,0.96031,0.96065,0.54091,0.54236,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5903,ERR1889860,ERX1950358,ERS1612624,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G12,SAMEA103923456,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923456|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G12|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:52020|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G12|scientific name:Danio rerio|side scatter:131|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G12 p,WT P5 G12 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N729_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N729_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,119506000.0,478024.0,E MTAB 5530:SLX 10882.N729 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:33161998;C:27138233;G:25209769;T:33994199;N:1801,125,125,,,33161998,27138233,25209769,33994199,1801,ERX1950358,ERS1612624,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.84785,0.8488,0.27109,0.27605,0.96773,0.96788,0.56847,0.58139,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5904,ERR1889859,ERX1950357,ERS1612623,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G11,SAMEA103923455,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923455|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G11|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:54830|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G11|scientific name:Danio rerio|side scatter:120|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G11 p,WT P5 G11 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N728_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N728_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,153779500.0,615118.0,E MTAB 5530:SLX 10882.N728 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:42335642;C:35194571;G:32871668;T:43375518;N:2101,125,125,,,42335642,35194571,32871668,43375518,2101,ERX1950357,ERS1612623,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.82012,0.82404,0.25128,0.25561,0.96575,0.96631,0.55979,0.55689,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5905,ERR1889858,ERX1950356,ERS1612622,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G10,SAMEA103923454,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923454|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G10|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:49213|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G10|scientific name:Danio rerio|side scatter:153|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G10 p,WT P5 G10 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N727_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N727_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,183923250.0,735693.0,E MTAB 5530:SLX 10882.N727 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:51867988;C:41059360;G:37881531;T:53112119;N:2252,125,125,,,51867988,41059360,37881531,53112119,2252,ERX1950356,ERS1612622,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.77315,0.77371,0.26428,0.26615,0.97035,0.97098,0.44222,0.55505,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5906,ERR1889857,ERX1950355,ERS1612621,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 G1,SAMEA103923453,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923453|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 G1|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:55940|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 G1|scientific name:Danio rerio|side scatter:136|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 G1 p,WT P5 G1 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N716_S521.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N716_S521.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,143299500.0,573198.0,E MTAB 5530:SLX 10882.N716 S521.C9FTNANXX.s 7.r ,0:125 1:125,A:39069921;C:33360092;G:30904716;T:39962896;N:1875,125,125,,,39069921,33360092,30904716,39962896,1875,ERX1950355,ERS1612621,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.80862,0.81082,0.21243,0.21625,0.95911,0.95966,0.46326,0.5832,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5907,ERR1889856,ERX1950354,ERS1612620,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F9,SAMEA103923452,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923452|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F9|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:55259|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F9|scientific name:Danio rerio|side scatter:108|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F9 p,WT P5 F9 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N726_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N726_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,187865000.0,751460.0,E MTAB 5530:SLX 10882.N726 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:53434735;C:41744767;G:38672065;T:54011263;N:2170,125,125,,,53434735,41744767,38672065,54011263,2170,ERX1950354,ERS1612620,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.887,0.88568,0.27918,0.29127,0.96244,0.96353,0.54479,0.53851,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5908,ERR1889855,ERX1950353,ERS1612619,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F8,SAMEA103923451,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923451|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F8|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:55042|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F8|scientific name:Danio rerio|side scatter:135|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F8 p,WT P5 F8 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N724_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N724_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,198382000.0,793528.0,E MTAB 5530:SLX 10882.N724 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:54697917;C:45579393;G:42534895;T:55567588;N:2207,125,125,,,54697917,45579393,42534895,55567588,2207,ERX1950353,ERS1612619,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.73676,0.73645,0.22283,0.22976,0.9653,0.96587,0.53398,0.54389,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5909,ERR1889854,ERX1950352,ERS1612618,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F7,SAMEA103923450,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923450|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F7|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:49480|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F7|scientific name:Danio rerio|side scatter:110|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F7 p,WT P5 F7 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N723_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N723_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,210633500.0,842534.0,E MTAB 5530:SLX 10882.N723 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:59185391;C:47136821;G:43900389;T:60408178;N:2721,125,125,,,59185391,47136821,43900389,60408178,2721,ERX1950352,ERS1612618,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.8684,0.86832,0.28159,0.29226,0.96952,0.97025,0.53175,0.5326,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5910,ERR1889853,ERX1950351,ERS1612617,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F6,SAMEA103923449,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923449|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F6|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:50172|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F6|scientific name:Danio rerio|side scatter:57|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F6 p,WT P5 F6 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N722_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N722_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,271533750.0,1086135.0,E MTAB 5530:SLX 10882.N722 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:73457611;C:63490411;G:59751088;T:74831830;N:2810,125,125,,,73457611,63490411,59751088,74831830,2810,ERX1950351,ERS1612617,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.77648,0.77134,0.22487,0.23077,0.97341,0.97374,0.56535,0.51475,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5911,ERR1889852,ERX1950350,ERS1612616,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F5,SAMEA103923448,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923448|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F5|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:58433|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F5|scientific name:Danio rerio|side scatter:120|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F5 p,WT P5 F5 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N721_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N721_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,139092250.0,556369.0,E MTAB 5530:SLX 10882.N721 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:36633152;C:33585349;G:31666956;T:37205060;N:1733,125,125,,,36633152,33585349,31666956,37205060,1733,ERX1950350,ERS1612616,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.90509,0.9025,0.10644,0.11841,0.95479,0.95603,0.53339,0.54004,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5912,ERR1889851,ERX1950349,ERS1612615,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F4,SAMEA103923447,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923447|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F4|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:54480|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F4|scientific name:Danio rerio|side scatter:120|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F4 p,WT P5 F4 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N720_S520.C9FTNANXX.s_7.r_2.fq.gz SLX-10882.N720_S520.C9FTNANXX.s_7.r_1.fq.gz,fastq fastq,204437750.0,817751.0,E MTAB 5530:SLX 10882.N720 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:53446720;C:49946054;G:47094381;T:53948287;N:2308,125,125,,,53446720,49946054,47094381,53948287,2308,ERX1950349,ERS1612615,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.89056,0.89016,0.12607,0.13775,0.97715,0.97808,0.49512,0.49352,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5913,ERR1889850,ERX1950348,ERS1612614,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F3,SAMEA103923446,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923446|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F3|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:50910|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F3|scientific name:Danio rerio|side scatter:93|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F3 p,WT P5 F3 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N719_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N719_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,274695000.0,1098780.0,E MTAB 5530:SLX 10882.N719 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:71915711;C:66760901;G:63125692;T:72889491;N:3205,125,125,,,71915711,66760901,63125692,72889491,3205,ERX1950348,ERS1612614,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.81536,0.81283,0.18017,0.1884,0.99017,0.9907,0.45256,0.45716,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5914,ERR1889849,ERX1950347,ERS1612613,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F2,SAMEA103923445,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923445|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F2|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:47559|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F2|scientific name:Danio rerio|side scatter:138|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F2 p,WT P5 F2 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N718_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N718_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,218505000.0,874020.0,E MTAB 5530:SLX 10882.N718 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:59587387;C:50598034;G:47629303;T:60687884;N:2392,125,125,,,59587387,50598034,47629303,60687884,2392,ERX1950347,ERS1612613,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.78996,0.78795,0.25659,0.26435,0.96844,0.96928,0.58397,0.57289,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5915,ERR1889848,ERX1950346,ERS1612612,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F12,SAMEA103923444,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923444|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F12|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:48572|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F12|scientific name:Danio rerio|side scatter:153|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F12 p,WT P5 F12 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N729_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N729_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,120451000.0,481804.0,E MTAB 5530:SLX 10882.N729 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:32637515;C:28176314;G:26300190;T:33335554;N:1427,125,125,,,32637515,28176314,26300190,33335554,1427,ERX1950346,ERS1612612,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.82878,0.82611,0.17623,0.19043,0.96065,0.96203,0.4916,0.50013,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5916,ERR1889847,ERX1950345,ERS1612611,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F11,SAMEA103923443,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923443|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F11|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:56221|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F11|scientific name:Danio rerio|side scatter:129|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F11 p,WT P5 F11 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N728_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N728_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,131249750.0,524999.0,E MTAB 5530:SLX 10882.N728 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:34519754;C:31827694;G:30048987;T:34851908;N:1407,125,125,,,34519754,31827694,30048987,34851908,1407,ERX1950345,ERS1612611,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.90351,0.90303,0.08867,0.10293,0.95954,0.96057,0.49218,0.49671,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5917,ERR1889846,ERX1950344,ERS1612610,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F10,SAMEA103923442,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923442|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F10|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:48863|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F10|scientific name:Danio rerio|side scatter:124|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F10 p,WT P5 F10 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N727_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N727_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,177934250.0,711737.0,E MTAB 5530:SLX 10882.N727 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:48471272;C:41462875;G:38595479;T:49402537;N:2087,125,125,,,48471272,41462875,38595479,49402537,2087,ERX1950344,ERS1612610,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.52645,0.52771,0.1361,0.14512,0.96504,0.96629,0.59554,0.57468,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5918,ERR1889845,ERX1950343,ERS1612609,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 F1,SAMEA103923441,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923441|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 F1|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:54789|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 F1|scientific name:Danio rerio|side scatter:147|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 F1 p,WT P5 F1 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N716_S520.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N716_S520.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,202956000.0,811824.0,E MTAB 5530:SLX 10882.N716 S520.C9FTNANXX.s 7.r ,0:125 1:125,A:55301859;C:47368588;G:44082805;T:56200278;N:2470,125,125,,,55301859,47368588,44082805,56200278,2470,ERX1950343,ERS1612609,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.74946,0.74466,0.20118,0.20905,0.95761,0.95844,0.53436,0.53353,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5919,ERR1889844,ERX1950342,ERS1612608,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 E9,SAMEA103923440,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923440|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 E9|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:56464|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 E9|scientific name:Danio rerio|side scatter:117|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 E9 p,WT P5 E9 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N726_S518.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N726_S518.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,337656750.0,1350627.0,E MTAB 5530:SLX 10882.N726 S518.C9FTNANXX.s 7.r ,0:125 1:125,A:92619597;C:78274106;G:72773857;T:93984314;N:4876,125,125,,,92619597,78274106,72773857,93984314,4876,ERX1950342,ERS1612608,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.82854,0.82898,0.22126,0.22374,0.97392,0.97467,0.55869,0.54845,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5920,ERR1889843,ERX1950341,ERS1612607,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 E8,SAMEA103923439,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923439|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 E8|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:48739|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 E8|scientific name:Danio rerio|side scatter:116|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 E8 p,WT P5 E8 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N724_S518.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N724_S518.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,203575750.0,814303.0,E MTAB 5530:SLX 10882.N724 S518.C9FTNANXX.s 7.r ,0:125 1:125,A:53819685;C:49152748;G:46331403;T:54269295;N:2619,125,125,,,53819685,49152748,46331403,54269295,2619,ERX1950341,ERS1612607,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.86887,0.87024,0.11906,0.12062,0.97751,0.97841,0.48144,0.48469,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5921,ERR1889842,ERX1950340,ERS1612606,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 E7,SAMEA103923438,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:11Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923438|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:11Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 E7|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:61339|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 E7|scientific name:Danio rerio|side scatter:107|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 E7 p,WT P5 E7 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N723_S518.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N723_S518.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,187593000.0,750372.0,E MTAB 5530:SLX 10882.N723 S518.C9FTNANXX.s 7.r ,0:125 1:125,A:50327297;C:44485106;G:41330297;T:51448428;N:1872,125,125,,,50327297,44485106,41330297,51448428,1872,ERX1950340,ERS1612606,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.81976,0.81904,0.13674,0.13885,0.9679,0.96855,0.52989,0.52724,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5922,ERR1889841,ERX1950339,ERS1612605,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 E6,SAMEA103923437,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923437|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 E6|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:49495|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 E6|scientific name:Danio rerio|side scatter:122|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 E6 p,WT P5 E6 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N722_S518.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N722_S518.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,272271500.0,1089086.0,E MTAB 5530:SLX 10882.N722 S518.C9FTNANXX.s 7.r ,0:125 1:125,A:75439324;C:61868919;G:57981653;T:76977665;N:3939,125,125,,,75439324,61868919,57981653,76977665,3939,ERX1950339,ERS1612605,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.83191,0.83204,0.2644,0.26611,0.968,0.96871,0.61636,0.61969,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5923,ERR1889840,ERX1950338,ERS1612604,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 E5,SAMEA103923436,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923436|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 E5|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:50378|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 E5|scientific name:Danio rerio|side scatter:130|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 E5 p,WT P5 E5 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N721_S518.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N721_S518.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,200315750.0,801263.0,E MTAB 5530:SLX 10882.N721 S518.C9FTNANXX.s 7.r ,0:125 1:125,A:52022363;C:49222409;G:46279394;T:52789541;N:2043,125,125,,,52022363,49222409,46279394,52789541,2043,ERX1950338,ERS1612604,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.91121,0.91291,0.09279,0.09428,0.96781,0.96859,0.49121,0.49938,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5924,ERR1889839,ERX1950337,ERS1612603,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 E4,SAMEA103923435,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923435|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 E4|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:50822|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 E4|scientific name:Danio rerio|side scatter:145|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 E4 p,WT P5 E4 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N720_S518.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N720_S518.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,204068000.0,816272.0,E MTAB 5530:SLX 10882.N720 S518.C9FTNANXX.s 7.r ,0:125 1:125,A:55843986;C:47157246;G:44069554;T:56994253;N:2961,125,125,,,55843986,47157246,44069554,56994253,2961,ERX1950337,ERS1612603,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.79028,0.79006,0.21556,0.21878,0.9627,0.96331,0.45599,0.57061,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system
5925,ERR1889838,ERX1950336,ERS1612602,ERP022169,PRJEB20058,Single cell RNA Seq data of zebrafish blood cells,E-MTAB-5530,Transcriptome Analysis,Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tgcd4:mCherry  Tgcd41:EGFP  Tggata1a:GFP  Tglyz:DsRed2  Tgmfap4:tdTomato  Tgmpx:EGFP  Tgrunx1:mCherry  Tgtal1:EGFP and Tubingen Long Fin wild type fish.,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2017 03 20|ArrayExpress:E MTAB 5530,,Protocols: A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,WT P5 E3,SAMEA103923434,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK",ENA FIRST PUBLIC:2017 11 17T17:02:10Z|ENA LAST UPDATE:2017 03 20T08:41:51Z|External Id:SAMEA103923434|INSDC center name:Department of Haematology  University of Cambridge  Cambridge  UK Wellcome Trust Sanger Institute  Wellcome Trust Genome Campus  Cambridge  UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute  Cambridge  CB2 1QR  UK|INSDC first public:2017 11 17T17:02:10Z|INSDC last update:2017 03 20T08:41:51Z|INSDC status:public|Submitter Id:E MTAB 5530:WT P5 E3|broker name:ArrayExpress|cell type:blood cell|common name:zebrafish|developmental stage:adult|forward scatter:50440|genotype:wild type genotype|organism part:kidney|reporter fluorescence:NaN|sample name:E MTAB 5530:WT P5 E3|scientific name:Danio rerio|side scatter:137|single cell well quality:OK|strain:Tubingen Long Fin|well information:1 cell,,,,,,,,,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,E MTAB 5530:WT P5 E3 p,WT P5 E3 p,Single cell RNA Seq data of zebrafish blood cells,A single kidney from heterozygous transgenic or wild type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4' 6 diamidino 2 phenylindole DAPI  Beckman Coulter  cat no B30437 for mCherry/dsRed2  or propidium iodide PI  Sigma cat no P4864 for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart seq2 protocol 27  28  with ERCC spike in controls added at the same time as the oligo dT and dNTP mixture dilution 1:10^7  according to manufacturer's instructions,Experimental Factor: wild type genotype:genotype|Experimental Factor: kidney:organism part|Experimental Factor: NaN:reporter fluorescence,RNA-Seq,TRANSCRIPTOMIC,cDNA,PAIRED,ILLUMINA,Illumina HiSeq 4000,<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>,ERP022169,Illumina HiSeq 4000 paired end sequencing; Single cell RNA Seq data of zebrafish blood cells,ENA FIRST PUBLIC:2017 11 17|ENA LAST UPDATE:2018 11 16,SLX-10882.N719_S518.C9FTNANXX.s_7.r_1.fq.gz SLX-10882.N719_S518.C9FTNANXX.s_7.r_2.fq.gz,fastq fastq,132152000.0,528608.0,E MTAB 5530:SLX 10882.N719 S518.C9FTNANXX.s 7.r ,0:125 1:125,A:36703908;C:29850306;G:27933919;T:37662323;N:1544,125,125,,,36703908,29850306,27933919,37662323,1544,ERX1950336,ERS1612602,ERA851041,"Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive","Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK|European Nucleotide Archive",2,0.82344,0.8259,0.21318,0.21568,0.94665,0.94771,0.60137,0.59972,125,125,B,B,biological fallback assumption,illumina,hiseq_era,unknown,poly_a,unknown,sc,single_cell_plate,smartseq,,United Kingdom,2017-03-20,Adult,Adult,Multi-tissue,Multi-system