rowid,query,bioproject,node,length,coverage,rel_abundance,taxname_lca,taxid_lca,taxoncategory,taxoncategorysimple,bits,evalue,lowcoverage_flag,analysis_used,target,taxid,gene,allele,pident,sumperc_cov,alnlen,mismatch,qcov,gapopen,qstart,qend,tstart,tend,target_title,analysis,sumalnlen,maxbits,bits_percmax,tax_superkingdom,tax_clade,tax_kingdom,tax_phylum,tax_class,tax_order,tax_family,tax_genus,tax_species
808791,PRJNA811237_S_NODE_30761_length_165_cov_0.879310_g30521_i0,PRJNA811237,30761,165,0.87931,1.4508615,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,106,5.51e-25,,NR,KAL0476844.1,1008807,g30521,i0,90.9,1,55,5,100,0,165,1,202,256,KAL0476844.1 succinate dehydrogenase (ubiquinone) flavoprotein subunit [Acrasis kona],diamond,165,106,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
2084463,PRJNA811237_S_NODE_14462_length_212_cov_2.503067_g14222_i0,PRJNA811237,14462,212,2.503067,5.30650204,Allovahlkampfia sp.,1874883,Heterolobosea,other_Eukaryota,342,9.879999999999999e-90,,NTclustered,gi|2651357779|gb|PP174295.1|,1874883,g14222,i0,95.775,3.00471698113208,213,8,100,1,1,212,3704,3916,"Allovahlkampfia sp. isolate P10 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence",blastn,637,342,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Allovahlkampfia,Allovahlkampfia sp.
2084493,PRJNA811237_S_NODE_25902_length_171_cov_1.213115_g25662_i0,PRJNA811237,25902,171,1.213115,2.07442665,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,86.7,1.96e-19,,NR,KAL0485467.1,1008807,g25662,i0,75.4,1,57,14,100,0,171,1,130,186,KAL0485467.1 ribosomal protein L17 [Acrasis kona],diamond,171,86.7,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
3359756,PRJNA811237_S_NODE_36043_length_159_cov_2.318182_g35803_i0,PRJNA811237,36043,159,2.318182,3.68590938,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,68.2,1.25e-11,,NR,KAL0481508.1,1008807,g35803,i0,66.7,2.77358490566038,51,17,96.2,0,159,7,286,336,KAL0481508.1 isobutyryl-CoA dehydrogenase [Acrasis kona],diamond,441,68.2,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
5912198,PRJNA811237_S_NODE_28145_length_167_cov_0.864407_g27905_i0,PRJNA811237,28145,167,0.864407,1.44355969,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,103,5.97e-26,,NR,KAL0486938.1,1008807,g27905,i0,87.3,2.92814371257485,55,7,98.8,0,165,1,44,98,KAL0486938.1 Ras-related protein Rap [Acrasis kona],diamond,489,103,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
7187067,PRJNA811237_S_NODE_26286_length_170_cov_1.173554_g26046_i0,PRJNA811237,26286,170,1.173554,1.9950418,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,102,9.66e-24,,NR,KAL0477514.1,1008807,g26046,i0,89.3,1.97647058823529,56,6,98.8,0,1,168,242,297,KAL0477514.1 HSP70 [Acrasis kona],diamond,336,102,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
8461844,PRJNA811237_S_NODE_26847_length_168_cov_1.705882_g26607_i0,PRJNA811237,26847,168,1.705882,2.86588176,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,159,8.08e-35,,NTclustered,gi|2124403222|ref|XM_044685950.1|,51637,g26607,i0,85.526,5.31547619047619,152,22,90,0,17,168,650,499,"Naegleria lovaniensis uncharacterized protein (C9374_010409), partial mRNA",blastn,893,159,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
9736815,PRJNA811237_S_NODE_10728_length_236_cov_2.502674_g10488_i0,PRJNA811237,10728,236,2.502674,5.90631064,Percolomonas cosmopolitus,63605,Heterolobosea,other_Eukaryota,289,1.48e-73,,NTclustered,gi|33089879|gb|AY283748.1|,63605,g10488,i0,89.474,99.2881355932203,228,24,97,0,9,236,493,266,"Percolomonas cosmopolitus isolate Gym67_1 actin gene, partial cds",blastn,23432,289,1,Eukaryota,Discoba,,Heterolobosea,,,Percolomonadidae,Percolomonas,Percolomonas cosmopolitus
11012689,PRJNA811237_S_NODE_1949_length_515_cov_2.092275_g1710_i0,PRJNA811237,1949,515,2.092275,10.77521625,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,241,8.16e-75,,NR,KAL0486486.1,1008807,g1710,i0,64.3,4.93980582524272,171,61,99.6,0,2,514,169,339,KAL0486486.1 citrate synthase [Acrasis kona],diamond,2544,241,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
11012734,PRJNA811237_S_NODE_36409_length_155_cov_2.613208_g36169_i0,PRJNA811237,36409,155,2.613208,4.0504724,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,73.1,9.78e-9,,NTclustered,gi|2119438032|ref|XM_044694250.1|,51637,g36169,i0,92.157,1.23225806451613,51,4,33,0,68,118,480,430,"Naegleria lovaniensis uncharacterized protein (C9374_004594), partial mRNA",blastn,191,73.1,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
12288168,PRJNA811237_S_NODE_5930_length_302_cov_1.201581_g5690_i0,PRJNA811237,5930,302,1.201581,3.62877462,Naegleria,5761,Heterolobosea,other_Eukaryota,109,1.74e-27,,NR,XP_002671268.1,5762,g5690,i0,54.3,2.53311258278146,94,43,93.4,0,11,292,18,111,XP_002671268.1 uncharacterized protein NAEGRDRAFT_73780 [Naegleria gruberi],diamond,765,109,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
13562371,PRJNA811237_S_NODE_19511_length_191_cov_1.380282_g19271_i0,PRJNA811237,19511,191,1.380282,2.63633862,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,106,7.95e-26,,NR,KAL0489712.1,1008807,g19271,i0,87.7,0.895287958115183,57,7,89.5,0,2,172,262,318,KAL0489712.1 heterotrimeric G-protein beta subunit [Acrasis kona],diamond,171,106,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
14836971,PRJNA811237_S_NODE_1452_length_603_cov_6.523466_g1222_i0,PRJNA811237,1452,603,6.523466,39.33649998,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,211,8.76e-66,,NR,XP_002679810.1,5762,g1222,i0,54,19.6318407960199,189,85,93,1,12,572,2,190,XP_002679810.1 60S ribosomal protein L9 [Naegleria gruberi],diamond,11838,211,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
15251640,PRJNA811237_S_NODE_452_length_1014_cov_5.951295_g289_i0,PRJNA811237,452,1014,5.951295,60.3461313,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,342,3.689999999999999e-112,,NR,XP_044563284.1,5763,g289,i0,51.4,3.68047337278107,311,151,92,0,2,934,96,406,XP_044563284.1 uncharacterized protein FDP41_002391 [Naegleria fowleri],diamond,3732,343,0.997084548104956,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
17387171,PRJNA811237_S_NODE_33654_length_162_cov_1.327434_g33414_i0,PRJNA811237,33654,162,1.327434,2.15044308,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,128,2.18e-25,,NTclustered,gi|2119420720|ref|XM_044689986.1|,51637,g33414,i0,80.864,95.4074074074074,162,31,100,0,1,162,899,1060,"Naegleria lovaniensis uncharacterized protein (C9374_014042), partial mRNA",blastn,15456,128,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
17801143,PRJNA811237_S_NODE_34074_length_162_cov_0.902655_g33834_i0,PRJNA811237,34074,162,0.902655,1.4623011,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,103,8.360000000000001e-27,,NR,KAL0490923.1,1008807,g33834,i0,90.6,0.981481481481482,53,5,98.1,0,161,3,47,99,KAL0490923.1 ribosomal protein S20 [Acrasis kona],diamond,159,103,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
19075233,PRJNA811237_S_NODE_31335_length_164_cov_0.886957_g31095_i0,PRJNA811237,31335,164,0.886957,1.45460948,Allovahlkampfia,1217108,Heterolobosea,other_Eukaryota,302,1.23e-77,,NTclustered,gi|89148133|gb|DQ388519.1|,373143,g31095,i0,100,25.6524390243902,163,0,99,0,2,164,306,468,"Heterolobosea sp. BA 16S small subunit ribosomal RNA gene, partial sequence; and His-Cys box homing endonuclease gene, complete cds",blastn,4207,303,0.996699669966997,Eukaryota,Discoba,,Heterolobosea,,,,,Heterolobosea sp. BA
19935411,PRJNA811237_S_NODE_17356_length_199_cov_2.313333_g17116_i0,PRJNA811237,17356,199,2.313333,4.60353267,Acrasis rosea,43695,Heterolobosea,other_Eukaryota,204,4.51e-48,,NTclustered,gi|12083876|gb|AF190771.1|AF190771,43695,g17116,i0,86.813,69.5628140703518,182,24,91,0,1,182,579,760,"Acrasis rosea elongation factor 1 alpha (tef1) gene, partial cds",blastn,13843,204,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis rosea
21210956,PRJNA811237_S_NODE_12657_length_223_cov_1.172414_g12417_i0,PRJNA811237,12657,223,1.172414,2.61448322,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,139,9.61e-39,,NR,KAL0489777.1,1008807,g12417,i0,86.5,0.995515695067265,74,10,99.6,0,222,1,97,170,KAL0489777.1 ribosomal protein S4 [Acrasis kona],diamond,222,139,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
21210988,PRJNA811237_S_NODE_2117_length_496_cov_1.747204_g1878_i0,PRJNA811237,2117,496,1.747204,8.66613184,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,163,2.6599999999999998e-46,,NR,AHW50664.1,5763,g1878,i0,53.4,1.76008064516129,148,61,85.9,5,65,490,28,173,AHW50664.1 cathepsin B-like protein [Naegleria fowleri],diamond,873,163,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
23761327,PRJNA811237_S_NODE_12039_length_227_cov_1.106742_g11799_i0,PRJNA811237,12039,227,1.106742,2.51230434,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,84.3,6.11e-17,,NR,KAL0484251.1,1008807,g11799,i0,56,1.98237885462555,75,33,99.1,0,3,227,391,465,KAL0484251.1 protein transport Sec61 alpha subunit [Acrasis kona],diamond,450,84.3,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
23761383,PRJNA811237_S_NODE_30499_length_165_cov_0.879310_g30259_i0,PRJNA811237,30499,165,0.87931,1.4508615,Allovahlkampfia sp.,1874883,Heterolobosea,other_Eukaryota,300,4.46e-77,,NTclustered,gi|2651357779|gb|PP174295.1|,1874883,g30259,i0,99.394,53.9939393939394,165,1,100,0,1,165,2662,2826,"Allovahlkampfia sp. isolate P10 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence",blastn,8909,300,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Allovahlkampfia,Allovahlkampfia sp.
25035260,PRJNA811237_S_NODE_28660_length_166_cov_1.128205_g28420_i0,PRJNA811237,28660,166,1.128205,1.8728203,Naegleria,5761,Heterolobosea,other_Eukaryota,108,5.1e-27,,NR,XP_002676783.1,5762,g28420,i0,90.7,4.89759036144578,54,5,97.6,0,5,166,6,59,XP_002676783.1 uncharacterized protein NAEGRDRAFT_55286 [Naegleria gruberi],diamond,813,108,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
25450201,PRJNA811237_S_NODE_11700_length_229_cov_1.244444_g11460_i0,PRJNA811237,11700,229,1.244444,2.84977676,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,137,1.08e-39,,NR,KAL0491536.1,1008807,g11460,i0,81.6,17.9213973799127,76,14,99.6,0,229,2,15,90,KAL0491536.1 ubiquitin conjugating enzyme E2 [Acrasis kona],diamond,4104,137,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
25450202,PRJNA811237_S_NODE_11960_length_227_cov_1.685393_g11720_i0,PRJNA811237,11960,227,1.685393,3.82584211,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,113,7.949999999999998e-30,,NR,KAL0487640.1,1008807,g11720,i0,70.3,0.977973568281938,74,22,97.8,0,226,5,48,121,KAL0487640.1 ribosomal protein L21 [Acrasis kona],diamond,222,113,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
26674044,PRJNA811237_S_NODE_11301_length_232_cov_1.114754_g11061_i0,PRJNA811237,11301,232,1.114754,2.58622928,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,92.8,3.75e-20,,NR,KAL0478728.1,1008807,g11061,i0,52,8.80603448275862,75,36,97,0,6,230,75,149,KAL0478728.1 actin-related protein 2 [Acrasis kona],diamond,2043,92.8,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
26705810,PRJNA811237_S_NODE_31161_length_164_cov_1.052174_g30921_i0,PRJNA811237,31161,164,1.052174,1.72556536,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,79.3,2.06e-16,,NR,KAL0479171.1,1008807,g30921,i0,63,0.98780487804878,54,20,98.8,0,2,163,60,113,KAL0479171.1 hypothetical protein AKO1_007994 [Acrasis kona],diamond,162,79.3,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
26769361,PRJNA811237_S_NODE_19841_length_190_cov_1.085106_g19601_i0,PRJNA811237,19841,190,1.085106,2.0617014,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,48.5,1.34e-4,,NR,XP_044569268.1,5763,g19601,i0,61.5,1.23157894736842,39,15,61.6,0,159,43,42,80,XP_044569268.1 uncharacterized protein FDP41_000454 [Naegleria fowleri],diamond,234,48.5,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
26769365,PRJNA811237_S_NODE_22561_length_181_cov_0.969697_g22321_i0,PRJNA811237,22561,181,0.969697,1.75515157,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,47.4,3.82e-4,,NR,CAG4712923.1,5763,g22321,i0,41.8,0.911602209944751,55,30,87.8,1,180,22,85,139,CAG4712923.1 unnamed protein product [Naegleria fowleri],diamond,165,47.4,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
26809954,PRJNA811237_S_NODE_24701_length_174_cov_1.632000_g24461_i0,PRJNA811237,24701,174,1.632,2.83968,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,72,3.82e-14,,NR,KAL0486596.1,1008807,g24461,i0,75,5.77586206896552,48,12,82.8,0,144,1,1,48,KAL0486596.1 ribosomal protein L26 [Acrasis kona],diamond,1005,72,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
26832756,PRJNA811237_S_NODE_10181_length_241_cov_1.593750_g9941_i0,PRJNA811237,10181,241,1.59375,3.8409375,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,96.3,4.559999999999999e-23,,NR,XP_044569726.1,5763,g9941,i0,95.8,0.597510373443983,48,2,59.8,0,240,97,116,163,XP_044569726.1 uncharacterized protein FDP41_000052 [Naegleria fowleri],diamond,144,96.3,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
26959150,PRJNA811237_S_NODE_33822_length_162_cov_0.902655_g33582_i0,PRJNA811237,33822,162,0.902655,1.4623011,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,65.1,8.33e-11,,NR,KAL0488247.1,1008807,g33582,i0,65.2,0.851851851851852,46,15,83.3,1,160,26,91,136,KAL0488247.1 aspartate carbamoyltransferase [Acrasis kona],diamond,138,65.1,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27022625,PRJNA811237_S_NODE_6442_length_291_cov_2.049587_g6202_i0,PRJNA811237,6442,291,2.049587,5.96429817,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,58.2,2.46e-7,,NR,KAL0480462.1,1008807,g6202,i0,38.4,1.68041237113402,86,53,88.7,0,282,25,78,163,KAL0480462.1 inter-alpha-trypsin inhibitor heavy chain H3 [Acrasis kona],diamond,489,58.2,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27054317,PRJNA811237_S_NODE_14562_length_212_cov_1.251534_g14322_i0,PRJNA811237,14562,212,1.251534,2.65325208,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,71.2,1.38e-12,,NR,XP_044556529.1,5763,g14322,i0,48.5,2.83018867924528,68,35,96.2,0,2,205,240,307,XP_044556529.1 uncharacterized protein FDP41_010036 [Naegleria fowleri],diamond,600,71.2,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
27085931,PRJNA811237_S_NODE_27982_length_167_cov_0.864407_g27742_i0,PRJNA811237,27982,167,0.864407,1.44355969,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,74.7,7.05e-14,,NR,XP_002672490.1,5762,g27742,i0,61.5,1.86826347305389,52,20,93.4,0,165,10,156,207,XP_002672490.1 transcription initiation factor TFIID subunit [Naegleria gruberi],diamond,312,74.7,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
27117245,PRJNA811237_S_NODE_30662_length_165_cov_0.879310_g30422_i0,PRJNA811237,30662,165,0.87931,1.4508615,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,115,2.79e-28,,NR,KAL0479791.1,1008807,g30422,i0,88.9,0.981818181818182,54,6,98.2,0,3,164,252,305,"KAL0479791.1 SLC5A, partial [Acrasis kona]",diamond,162,115,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27180424,PRJNA811237_S_NODE_1662_length_564_cov_3.974757_g1425_i0,PRJNA811237,1662,564,3.974757,22.41762948,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,170,1.89e-49,,NR,XP_044546422.1,51637,g1425,i0,55,0.792553191489362,149,66,79.3,1,510,64,86,233,XP_044546422.1 uncharacterized protein C9374_007798 [Naegleria lovaniensis],diamond,447,170,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
27212088,PRJNA811237_S_NODE_11502_length_230_cov_2.254144_g11262_i0,PRJNA811237,11502,230,2.254144,5.1845312,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,112,2.32e-27,,NR,KAL0484165.1,1008807,g11262,i0,68.4,0.991304347826087,76,24,99.1,0,1,228,234,309,KAL0484165.1 enolase [Acrasis kona],diamond,228,112,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27212096,PRJNA811237_S_NODE_21222_length_185_cov_1.352941_g20982_i0,PRJNA811237,21222,185,1.352941,2.50294085,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,68.2,5.02e-12,,NR,CAG4717580.1,5763,g20982,i0,39.3,0.989189189189189,61,37,98.9,0,184,2,35,95,CAG4717580.1 unnamed protein product [Naegleria fowleri],diamond,183,68.2,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
27243872,PRJNA811237_S_NODE_24863_length_174_cov_1.224000_g24623_i0,PRJNA811237,24863,174,1.224,2.12976,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,77.8,2.21e-15,,NR,XP_044555785.1,51637,g24623,i0,57.1,2.89655172413793,56,24,96.6,0,171,4,88,143,XP_044555785.1 uncharacterized protein C9374_003655 [Naegleria lovaniensis],diamond,504,77.8,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
27243874,PRJNA811237_S_NODE_27343_length_167_cov_1.296610_g27103_i0,PRJNA811237,27343,167,1.29661,2.1653387,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,91.3,1.06e-19,,NR,KAL0481918.1,1008807,g27103,i0,83.6,0.988023952095808,55,9,98.8,0,3,167,135,189,KAL0481918.1 vacuolar protein sorting protein VPS31 [Acrasis kona],diamond,165,91.3,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27243878,PRJNA811237_S_NODE_33423_length_162_cov_2.176991_g33183_i0,PRJNA811237,33423,162,2.176991,3.52672542,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,55.1,5.35e-7,,NR,XP_044568892.1,5763,g33183,i0,49,0.907407407407407,49,25,90.7,0,3,149,293,341,XP_044568892.1 uncharacterized protein FDP41_007356 [Naegleria fowleri],diamond,147,55.1,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
27307267,PRJNA811237_S_NODE_23323_length_178_cov_1.775194_g23083_i0,PRJNA811237,23323,178,1.775194,3.15984532,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,95.5,1.4399999999999994e-23,,NR,KAL0487562.1,1008807,g23083,i0,78,0.99438202247191,59,13,99.4,0,1,177,7,65,KAL0487562.1 profilin [Acrasis kona],diamond,177,95.5,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27316134,PRJNA811237_S_NODE_32543_length_163_cov_0.894737_g32303_i0,PRJNA811237,32543,163,0.894737,1.45842131,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,76.6,1.42e-14,,NR,XP_044542536.1,51637,g32303,i0,68.6,11.6319018404908,51,16,93.9,0,162,10,784,834,XP_044542536.1 uncharacterized protein C9374_012228 [Naegleria lovaniensis],diamond,1896,76.6,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
27338844,PRJNA811237_S_NODE_23863_length_177_cov_0.867188_g23623_i0,PRJNA811237,23863,177,0.867188,1.53492276,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,52.4,3.85e-6,,NR,KAL0481873.1,1008807,g23623,i0,47.2,0.898305084745763,53,25,89.8,2,17,175,1,50,KAL0481873.1 hypothetical protein AKO1_011303 [Acrasis kona],diamond,159,52.4,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27528153,PRJNA811237_S_NODE_15203_length_209_cov_0.956250_g14963_i0,PRJNA811237,15203,209,0.95625,1.9985625,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,85.1,2.8e-17,,NR,KAL0484804.1,1008807,g14963,i0,56.7,0.961722488038278,67,29,96.2,0,206,6,454,520,KAL0484804.1 ctnA [Acrasis kona],diamond,201,85.1,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27623088,PRJNA811237_S_NODE_10064_length_242_cov_1.927461_g9824_i0,PRJNA811237,10064,242,1.927461,4.66445562,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,85.1,4.25e-19,,NR,KAL0483908.1,1008807,g9824,i0,54.1,0.91735537190082606,74,34,91.7,0,222,1,1,74,KAL0483908.1 profilin [Acrasis kona],diamond,222,85.1,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27623093,PRJNA811237_S_NODE_13704_length_217_cov_0.910714_g13464_i0,PRJNA811237,13704,217,0.910714,1.97624938,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,119,1.32e-30,,NR,KAL0483913.1,1008807,g13464,i0,77.8,0.995391705069124,72,16,99.5,0,217,2,163,234,KAL0483913.1 ribosomal protein P0 [Acrasis kona],diamond,216,119,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27654683,PRJNA811237_S_NODE_7944_length_266_cov_1.465438_g7704_i0,PRJNA811237,7944,266,1.465438,3.89806508,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,120,3.47e-33,,NR,KAL0486015.1,1008807,g7704,i0,69.3,1.95112781954887,88,25,99.2,1,266,3,11,96,KAL0486015.1 ribosomal protein S26 [Acrasis kona],diamond,519,120,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27718059,PRJNA811237_S_NODE_17764_length_198_cov_1.026846_g17524_i0,PRJNA811237,17764,198,1.026846,2.03315508,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,60.8,8.31e-9,,NR,XP_044545513.1,51637,g17524,i0,46,0.954545454545455,63,34,95.5,0,196,8,175,237,XP_044545513.1 uncharacterized protein C9374_008394 [Naegleria lovaniensis],diamond,189,60.8,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
27718068,PRJNA811237_S_NODE_28964_length_166_cov_0.871795_g28724_i0,PRJNA811237,28964,166,0.871795,1.4471797,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,68.2,1.42e-11,,NR,XP_002683441.1,5762,g28724,i0,59.3,2.92771084337349,54,22,97.6,0,166,5,391,444,XP_002683441.1 uncharacterized protein NAEGRDRAFT_34629 [Naegleria gruberi],diamond,486,68.2,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
27726921,PRJNA811237_S_NODE_5204_length_319_cov_1.496296_g4964_i0,PRJNA811237,5204,319,1.496296,4.77318424,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,114,4.46e-28,,NR,AGE43966.1,5763,g4964,i0,49.1,2.96238244514107,106,53,99.7,1,2,319,173,277,AGE43966.1 putative cathepsin B [Naegleria fowleri],diamond,945,115,0.991304347826087,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
27749471,PRJNA811237_S_NODE_33284_length_163_cov_0.894737_g33044_i0,PRJNA811237,33284,163,0.894737,1.45842131,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,95.5,2e-21,,NR,KAL0483871.1,1008807,g33044,i0,81.5,0.993865030674847,54,10,99.4,0,1,162,152,205,KAL0483871.1 malate dehydrogenase [Acrasis kona],diamond,162,95.5,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27812613,PRJNA811237_S_NODE_34104_length_162_cov_0.902655_g33864_i0,PRJNA811237,34104,162,0.902655,1.4623011,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,46.2,7.26e-4,,NR,XP_044545604.1,51637,g33864,i0,45.1,1.92592592592593,51,27,92.6,1,160,11,38,88,XP_044545604.1 uncharacterized protein C9374_008485 [Naegleria lovaniensis],diamond,312,46.2,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
27852685,PRJNA811237_S_NODE_10504_length_238_cov_1.904762_g10264_i0,PRJNA811237,10504,238,1.904762,4.53333356,root,1,Root_unresolved,Root_unresolved,99.4,1.92e-23,,NR,XP_002677917.1,5762,g10264,i0,64.2,2.53361344537815,67,24,84.5,0,238,38,159,225,"XP_002677917.1 uncharacterized protein NAEGRDRAFT_33131, partial [Naegleria gruberi]",diamond,603,99.8,0.995991983967936,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
27875410,PRJNA811237_S_NODE_13425_length_218_cov_1.810651_g13185_i0,PRJNA811237,13425,218,1.810651,3.94721918,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,136,2.0399999999999997e-37,,NR,AHW50664.1,5763,g13185,i0,79.2,2.97247706422018,72,15,99.1,0,1,216,94,165,AHW50664.1 cathepsin B-like protein [Naegleria fowleri],diamond,648,136,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
27875443,PRJNA811237_S_NODE_9225_length_251_cov_1.628713_g8985_i0,PRJNA811237,9225,251,1.628713,4.08806963,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,125,7.93e-35,,NR,XP_044564077.1,5763,g8985,i0,72.4,0.908366533864542,76,21,90.8,0,1,228,76,151,XP_044564077.1 uncharacterized protein FDP41_001707 [Naegleria fowleri],diamond,228,125,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
27970196,PRJNA811237_S_NODE_28845_length_166_cov_0.871795_g28605_i0,PRJNA811237,28845,166,0.871795,1.4471797,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,101,2.87e-23,,NR,KAL0479622.1,1008807,g28605,i0,90.7,0.975903614457831,54,5,97.6,0,5,166,488,541,KAL0479622.1 hypothetical protein AKO1_007712 [Acrasis kona],diamond,162,101,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
27970197,PRJNA811237_S_NODE_29505_length_166_cov_0.871795_g29265_i0,PRJNA811237,29505,166,0.871795,1.4471797,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,98.2,1.67e-24,,NR,KAL0491353.1,1008807,g29265,i0,90.9,0.993975903614458,55,5,99.4,0,165,1,80,134,KAL0491353.1 ribosomal protein S16 [Acrasis kona],diamond,165,98.2,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
28033546,PRJNA811237_S_NODE_19265_length_192_cov_1.363636_g19025_i0,PRJNA811237,19265,192,1.363636,2.61818112,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,55.5,5.91e-7,,NR,XP_002676091.1,5762,g19025,i0,51.2,1.328125,41,20,64.1,0,162,40,257,297,XP_002676091.1 uncharacterized protein NAEGRDRAFT_34402 [Naegleria gruberi],diamond,255,55.5,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28191034,PRJNA811237_S_NODE_29706_length_165_cov_1.629310_g29466_i0,PRJNA811237,29706,165,1.62931,2.6883615,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,62.8,6.34e-10,,NR,KAL0477522.1,1008807,g29466,i0,52.8,1.94545454545455,53,25,96.4,0,6,164,14,66,KAL0477522.1 3-hydroxyacyl-CoA dehydrogenase [Acrasis kona],diamond,321,62.8,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
28231383,PRJNA811237_S_NODE_24126_length_176_cov_1.204724_g23886_i0,PRJNA811237,24126,176,1.204724,2.12031424,Naegleria,5761,Heterolobosea,other_Eukaryota,89.4,1.29e-19,,NR,XP_002675028.1,5762,g23886,i0,70.7,1.97727272727273,58,16,97.2,1,4,174,124,181,XP_002675028.1 uncharacterized protein NAEGRDRAFT_58587 [Naegleria gruberi],diamond,348,89.7,0.996655518394649,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28285775,PRJNA811237_S_NODE_30226_length_165_cov_0.879310_g29986_i0,PRJNA811237,30226,165,0.87931,1.4508615,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,61.2,3.78e-9,,NR,KAL0490859.1,1008807,g29986,i0,59.3,0.981818181818182,54,22,98.2,0,2,163,770,823,"KAL0490859.1 aminopeptidase, partial [Acrasis kona]",diamond,162,61.2,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
28294727,PRJNA811237_S_NODE_19326_length_192_cov_1.069930_g19086_i0,PRJNA811237,19326,192,1.06993,2.0542656,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,127,7.58e-34,,NR,XP_002668652.1,5762,g19086,i0,90.6,9,64,6,100,0,1,192,33,96,XP_002668652.1 uncharacterized protein NAEGRDRAFT_60075 [Naegleria gruberi],diamond,1728,128,0.9921875,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28317551,PRJNA811237_S_NODE_18706_length_194_cov_1.406897_g18466_i0,PRJNA811237,18706,194,1.406897,2.72938018,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,78.2,1.72e-16,,NR,XP_002676892.1,5762,g18466,i0,65.1,0.974226804123711,63,22,97.4,0,6,194,76,138,"XP_002676892.1 uncharacterized protein NAEGRDRAFT_33801, partial [Naegleria gruberi]",diamond,189,78.2,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28357780,PRJNA811237_S_NODE_19886_length_190_cov_0.985816_g19646_i0,PRJNA811237,19886,190,0.985816,1.8730504,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,85.1,5.6e-18,,NR,XP_002672577.1,5762,g19646,i0,62.9,33.8526315789474,62,23,97.9,0,3,188,72,133,XP_002672577.1 uncharacterized protein NAEGRDRAFT_81157 [Naegleria gruberi],diamond,6432,85.5,0.9953216374269,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28380679,PRJNA811237_S_NODE_26046_length_170_cov_1.950413_g25806_i0,PRJNA811237,26046,170,1.950413,3.3157021,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,83.6,5.66e-17,,NR,KAL0487709.1,1008807,g25806,i0,60.7,3.91764705882353,56,22,98.8,0,168,1,360,415,KAL0487709.1 valine tRS [Acrasis kona],diamond,666,83.6,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
28389442,PRJNA811237_S_NODE_23066_length_179_cov_1.538462_g22826_i0,PRJNA811237,23066,179,1.538462,2.75384698,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,80.9,5.61e-16,,NR,XP_002681901.1,5762,g22826,i0,69.6,6.77094972067039,56,17,93.9,0,1,168,346,401,XP_002681901.1 methylmalonate-semialdehyde dehydrogenase [Naegleria gruberi],diamond,1212,81.3,0.995079950799508,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28412346,PRJNA811237_S_NODE_27046_length_168_cov_1.058824_g26806_i0,PRJNA811237,27046,168,1.058824,1.77882432,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,41.2,0.0473,,NR,KAL0486710.1,1008807,g26806,i0,43.5,0.821428571428571,46,26,82.1,0,3,140,418,463,KAL0486710.1 developmentally-regulated external PM-anchored protein [Acrasis kona],diamond,138,41.2,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
28444001,PRJNA811237_S_NODE_24546_length_175_cov_1.047619_g24306_i0,PRJNA811237,24546,175,1.047619,1.83333325,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,47.4,3.58e-4,,NR,XP_002673672.1,5762,g24306,i0,42,0.857142857142857,50,29,85.7,0,174,25,1123,1172,XP_002673672.1 component of scar regulatory complex [Naegleria gruberi],diamond,150,47.4,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28444007,PRJNA811237_S_NODE_30526_length_165_cov_0.879310_g30286_i0,PRJNA811237,30526,165,0.87931,1.4508615,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,97.8,1.32e-22,,NR,KAL0478092.1,1008807,g30286,i0,78.2,1,55,11,98.2,1,163,2,106,160,KAL0478092.1 guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta [Acrasis kona],diamond,165,97.8,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
28475476,PRJNA811237_S_NODE_5666_length_307_cov_2.240310_g5426_i0,PRJNA811237,5666,307,2.24031,6.8777517,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,114,3.33e-29,,NR,KAL0489093.1,1008807,g5426,i0,63.7,0.996742671009772,102,28,98.7,3,303,1,104,197,KAL0489093.1 ribosomal protein S8 [Acrasis kona],diamond,306,114,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
28484311,PRJNA811237_S_NODE_30546_length_165_cov_0.879310_g30306_i0,PRJNA811237,30546,165,0.87931,1.4508615,Naegleria,5761,Heterolobosea,other_Eukaryota,84.7,2.13e-17,,NR,CAG4716440.1,5763,g30306,i0,70.9,3,55,16,100,0,165,1,454,508,"CAG4716440.1 unnamed protein product, partial [Naegleria fowleri]",diamond,495,84.7,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
28515891,PRJNA811237_S_NODE_17007_length_201_cov_1.230263_g16767_i0,PRJNA811237,17007,201,1.230263,2.47282863,root,1,Root_unresolved,Root_unresolved,92,2.38e-20,,NR,XP_002675106.1,5762,g16767,i0,66.7,3.91044776119403,66,21,97,1,199,5,253,318,XP_002675106.1 uncharacterized protein NAEGRDRAFT_80430 [Naegleria gruberi],diamond,786,92,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28570085,PRJNA811237_S_NODE_20707_length_187_cov_1.108696_g20467_i0,PRJNA811237,20707,187,1.108696,2.07326152,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,101,3.19e-23,,NR,XP_044544774.1,51637,g20467,i0,84.7,0.946524064171123,59,9,94.7,0,186,10,614,672,XP_044544774.1 uncharacterized protein C9374_009028 [Naegleria lovaniensis],diamond,177,101,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
28570096,PRJNA811237_S_NODE_34047_length_162_cov_0.902655_g33807_i0,PRJNA811237,34047,162,0.902655,1.4623011,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,83.6,3.06e-17,,NR,CAG4719649.1,5763,g33807,i0,64.8,2.92592592592593,54,18,98.1,1,161,3,166,219,CAG4719649.1 unnamed protein product [Naegleria fowleri],diamond,474,83.6,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
28633486,PRJNA811237_S_NODE_12647_length_223_cov_1.172414_g12407_i0,PRJNA811237,12647,223,1.172414,2.61448322,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,55.1,1.28e-6,,NR,XP_044567015.1,5763,g12407,i0,42.9,0.847533632286996,63,36,84.8,0,190,2,292,354,XP_044567015.1 uncharacterized protein FDP41_011232 [Naegleria fowleri],diamond,189,55.1,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
28633506,PRJNA811237_S_NODE_28027_length_167_cov_0.864407_g27787_i0,PRJNA811237,28027,167,0.864407,1.44355969,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,75.5,1.38e-14,,NR,KAL0488032.1,1008807,g27787,i0,68.6,0.916167664670659,51,16,91.6,0,10,162,207,257,KAL0488032.1 villin [Acrasis kona],diamond,153,75.5,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
28633510,PRJNA811237_S_NODE_34087_length_162_cov_0.902655_g33847_i0,PRJNA811237,34087,162,0.902655,1.4623011,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,96.7,1.6399999999999998e-23,,NR,XP_002681260.1,5762,g33847,i0,79.6,5,54,11,100,0,1,162,13,66,XP_002681260.1 cyclophilin [Naegleria gruberi],diamond,810,96.7,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28728057,PRJNA811237_S_NODE_4847_length_329_cov_1.860714_g4607_i0,PRJNA811237,4847,329,1.860714,6.12174906,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,95.5,2.2700000000000004e-21,,NR,XP_044551761.1,51637,g4607,i0,43.7,0.939209726443769,103,54,93.9,3,21,329,80,178,XP_044551761.1 uncharacterized protein C9374_001363 [Naegleria lovaniensis],diamond,309,95.5,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
28926204,PRJNA811237_S_NODE_29148_length_166_cov_0.871795_g28908_i0,PRJNA811237,29148,166,0.871795,1.4471797,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,65.5,6.36e-11,,NR,XP_002673591.1,5762,g28908,i0,57.4,1.95180722891566,54,23,97.6,0,3,164,33,86,XP_002673591.1 gamma carbonic dehydratase [Naegleria gruberi],diamond,324,65.9,0.993930197268589,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
28949272,PRJNA811237_S_NODE_28248_length_167_cov_0.864407_g28008_i0,PRJNA811237,28248,167,0.864407,1.44355969,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,75.9,2.76e-14,,NR,KAL0484804.1,1008807,g28008,i0,58.5,0.952095808383233,53,22,95.2,0,167,9,460,512,KAL0484804.1 ctnA [Acrasis kona],diamond,159,75.9,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
29106990,PRJNA811237_S_NODE_16688_length_202_cov_1.666667_g16448_i0,PRJNA811237,16688,202,1.666667,3.36666734,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,60.8,1.14e-9,,NR,XP_002676892.1,5762,g16448,i0,68.4,1.72277227722772,38,12,56.4,0,198,85,103,140,"XP_002676892.1 uncharacterized protein NAEGRDRAFT_33801, partial [Naegleria gruberi]",diamond,348,60.8,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
29210612,PRJNA811237_S_NODE_26429_length_169_cov_1.833333_g26189_i0,PRJNA811237,26429,169,1.833333,3.09833277,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,70.9,3.16e-13,,NR,XP_002674045.1,5762,g26189,i0,70.6,6.65680473372781,51,15,90.5,0,169,17,3,53,XP_002674045.1 uncharacterized protein NAEGRDRAFT_80713 [Naegleria gruberi],diamond,1125,70.9,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
29327847,PRJNA811237_S_NODE_30549_length_165_cov_0.879310_g30309_i0,PRJNA811237,30549,165,0.87931,1.4508615,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,79,2.05e-15,,NR,KAL0490498.1,1008807,g30309,i0,69.8,0.963636363636364,53,16,96.4,0,5,163,361,413,KAL0490498.1 peptide chain release factor subunit 3 [Acrasis kona],diamond,159,79,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
29336774,PRJNA811237_S_NODE_10329_length_240_cov_1.319372_g10089_i0,PRJNA811237,10329,240,1.319372,3.1664928,Naegleria,5761,Heterolobosea,other_Eukaryota,73.6,1.02e-13,,NR,XP_002672291.1,5762,g10089,i0,48.7,7.4875,78,40,97.5,0,237,4,50,127,XP_002672291.1 uncharacterized protein NAEGRDRAFT_72744 [Naegleria gruberi],diamond,1797,73.9,0.995940460081191,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
29399845,PRJNA811237_S_NODE_21229_length_185_cov_1.264706_g20989_i0,PRJNA811237,21229,185,1.264706,2.3397061,Naegleria,5761,Heterolobosea,other_Eukaryota,117,7.02e-30,,NR,XP_002683348.1,5762,g20989,i0,91.7,6.85945945945946,60,5,97.3,0,182,3,183,242,XP_002683348.1 uncharacterized protein NAEGRDRAFT_82392 [Naegleria gruberi],diamond,1269,117,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
29611770,PRJNA811237_S_NODE_19750_length_190_cov_1.446809_g19510_i0,PRJNA811237,19750,190,1.446809,2.7489371,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,73.6,1.17e-14,,NR,KAL0480477.1,1008807,g19510,i0,82.2,0.710526315789474,45,8,71.1,0,139,5,4,48,KAL0480477.1 ribosomal protein L27 [Acrasis kona],diamond,135,73.6,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
29674911,PRJNA811237_S_NODE_20330_length_188_cov_1.467626_g20090_i0,PRJNA811237,20330,188,1.467626,2.75913688,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,78.6,4.22e-15,,NR,KAL0477447.1,1008807,g20090,i0,62.3,0.973404255319149,61,23,97.3,0,183,1,749,809,KAL0477447.1 COPI beta [Acrasis kona],diamond,183,78.6,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
29738098,PRJNA811237_S_NODE_11130_length_233_cov_1.891304_g10890_i0,PRJNA811237,11130,233,1.891304,4.40673832,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,115,6.82e-28,,NR,KAL0483360.1,1008807,g10890,i0,65.3,0.965665236051502,75,26,96.6,0,9,233,105,179,KAL0483360.1 prolyl tRS [Acrasis kona],diamond,225,115,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
29801409,PRJNA811237_S_NODE_17991_length_197_cov_1.209459_g17751_i0,PRJNA811237,17991,197,1.209459,2.38263423,Naegleria lovaniensis,51637,Heterolobosea,other_Eukaryota,49.7,7.03e-5,,NR,XP_044555993.1,51637,g17751,i0,47.5,0.609137055837564,40,21,60.9,0,185,66,32,71,XP_044555993.1 uncharacterized protein C9374_003863 [Naegleria lovaniensis],diamond,120,49.7,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
29833094,PRJNA811237_S_NODE_1171_length_657_cov_3.799342_g953_i0,PRJNA811237,1171,657,3.799342,24.96167694,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,76.3,3.57e-14,,NR,XP_044563804.1,5763,g953,i0,51.5,0.310502283105023,68,33,31.1,0,262,59,24,91,XP_044563804.1 uncharacterized protein FDP41_002161 [Naegleria fowleri],diamond,204,76.3,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
30000023,PRJNA811237_S_NODE_23051_length_179_cov_1.569231_g22811_i0,PRJNA811237,23051,179,1.569231,2.80892349,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,87.4,2.13e-18,,NR,XP_044555909.1,51637,g22811,i0,64.4,2.96648044692737,59,21,98.9,0,179,3,208,266,XP_044555909.1 uncharacterized protein C9374_003779 [Naegleria lovaniensis],diamond,531,87.4,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
30022842,PRJNA811237_S_NODE_32831_length_163_cov_0.894737_g32591_i0,PRJNA811237,32831,163,0.894737,1.45842131,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,43.9,0.00471,,NR,KAL0479973.1,1008807,g32591,i0,44.2,1.43558282208589,43,22,75.5,1,128,6,87,129,KAL0479973.1 metA [Acrasis kona],diamond,234,43.9,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
30062942,PRJNA811237_S_NODE_21131_length_185_cov_1.875000_g20891_i0,PRJNA811237,21131,185,1.875,3.46875,root,1,Root_unresolved,Root_unresolved,47.4,6.47e-5,,NR,KAL0486669.1,1008807,g20891,i0,45.6,2.3027027027027,57,30,90.8,1,4,171,40,96,KAL0486669.1 developmentally-regulated external PM-anchored protein [Acrasis kona],diamond,426,47.4,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
30085737,PRJNA811237_S_NODE_25592_length_172_cov_1.040650_g25352_i0,PRJNA811237,25592,172,1.04065,1.789918,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,73.9,7.78e-14,,NR,XP_002676718.1,5762,g25352,i0,67.3,0.959302325581395,55,18,95.9,0,1,165,45,99,XP_002676718.1 uncharacterized protein NAEGRDRAFT_67999 [Naegleria gruberi],diamond,165,73.9,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
30126047,PRJNA811237_S_NODE_29092_length_166_cov_0.871795_g28852_i0,PRJNA811237,29092,166,0.871795,1.4471797,Eukaryota,2759,unresolved_Eukaryota,other_Eukaryota,59.7,1.38e-8,,NR,XP_044542409.1,51637,g28852,i0,55.8,6.6144578313253,52,23,94,0,162,7,612,663,XP_044542409.1 uncharacterized protein C9374_012338 [Naegleria lovaniensis],diamond,1098,60.1,0.993344425956739,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria lovaniensis
30148826,PRJNA811237_S_NODE_24332_length_175_cov_1.619048_g24092_i0,PRJNA811237,24332,175,1.619048,2.833334,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,45.8,0.00123,,NR,KAL0484459.1,1008807,g24092,i0,42.6,1.16571428571429,68,28,97.7,1,175,5,252,319,KAL0484459.1 legumain [Acrasis kona],diamond,204,45.8,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona
30212062,PRJNA811237_S_NODE_28932_length_166_cov_0.871795_g28692_i0,PRJNA811237,28932,166,0.871795,1.4471797,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,42.4,0.0177,,NR,XP_002676807.1,5762,g28692,i0,48.7,0.704819277108434,39,18,66.9,1,6,116,137,175,XP_002676807.1 uncharacterized protein NAEGRDRAFT_49342 [Naegleria gruberi],diamond,117,42.4,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
30243953,PRJNA811237_S_NODE_25492_length_172_cov_1.243902_g25252_i0,PRJNA811237,25492,172,1.243902,2.13951144,Acrasis kona,1008807,Heterolobosea,other_Eukaryota,48.5,7.78e-5,,NR,KAL0482136.1,1008807,g25252,i0,63.5,0.906976744186046,52,16,87.2,2,170,21,140,190,KAL0482136.1 ubiquitin-small subunit ribosomal protein S27 [Acrasis kona],diamond,156,48.5,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Acrasis,Acrasis kona