rowid,query,bioproject,node,length,coverage,rel_abundance,taxname_lca,taxid_lca,taxoncategory,taxoncategorysimple,bits,evalue,lowcoverage_flag,analysis_used,target,taxid,gene,allele,pident,sumperc_cov,alnlen,mismatch,qcov,gapopen,qstart,qend,tstart,tend,target_title,analysis,sumalnlen,maxbits,bits_percmax,tax_superkingdom,tax_clade,tax_kingdom,tax_phylum,tax_class,tax_order,tax_family,tax_genus,tax_species
450104,PRJNA488043_S_NODE_1241_length_173_cov_9.687500_g1230_i0,PRJNA488043,1241,173,9.6875,16.759375,Naegleria jadini,51636,Heterolobosea,other_Eukaryota,311,2.17e-80,,NTclustered,gi|2438257170|gb|OP997288.1|,51636,g1230,i0,99.419,9.80346820809249,172,0,99,1,2,173,6229,6059,"Naegleria jadini strain ITMAP400 plasmid CERE, complete sequence",blastn,1696,311,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria jadini
450111,PRJNA488043_S_NODE_1661_length_150_cov_2.768595_g1650_i0,PRJNA488043,1661,150,2.768595,4.1528925,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,145,1.9599999999999997e-30,,NTclustered,gi|290998100|ref|XM_002681573.1|,5762,g1650,i0,88.618,0.82,123,9,81,3,16,137,359,241,"Naegleria gruberi 60S acidic ribosomal protein P1 (NAEGRDRAFT_78273), mRNA",blastn,123,145,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
450114,PRJNA488043_S_NODE_441_length_278_cov_3.939759_g430_i0,PRJNA488043,441,278,3.939759,10.95253002,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,449,7.77e-122,,NTclustered,gi|290975864|ref|XM_002670616.1|,5762,g430,i0,96.029,14.1762589928058,277,9,99,2,3,278,365,640,"Naegleria gruberi uncharacterized protein (NAEGRDRAFT_55913), mRNA",blastn,3941,449,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
1724766,PRJNA488043_S_NODE_982_length_192_cov_2.558282_g971_i0,PRJNA488043,982,192,2.558282,4.91190144,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,333,5.269999999999999e-87,,NTclustered,gi|290980449|ref|XM_002672899.1|,5762,g971,i0,97.917,2.95833333333333,192,4,100,0,1,192,693,502,"Naegleria gruberi uncharacterized protein (NAEGRDRAFT_59104), mRNA",blastn,568,333,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
3000533,PRJNA488043_P_NODE_10703_length_317_cov_3.635417_g8462_i0,PRJNA488043,10703,317,3.635417,11.52427189,Allovahlkampfia sp.,1874883,Heterolobosea,other_Eukaryota,479,1.1499999999999999e-130,,NTclustered,gi|2651357779|gb|PP174295.1|,1874883,g8462,i0,94.006,12.3343848580442,317,17,100,2,1,317,3925,3611,"Allovahlkampfia sp. isolate P10 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence",blastn,3910,479,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Allovahlkampfia,Allovahlkampfia sp.
3000767,PRJNA488043_S_NODE_1063_length_185_cov_10.955128_g1052_i0,PRJNA488043,1063,185,10.955128,20.2669868,Naegleria jadini,51636,Heterolobosea,other_Eukaryota,342,8.369999999999999e-90,,NTclustered,gi|38606346|gb|AY376154.1|,51636,g1052,i0,100,87.8324324324324,185,0,100,0,1,185,924,740,"Naegleria jadini isolate 7858 small subunit ribosomal RNA gene, partial sequence; mitochondrial",blastn,16249,342,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria jadini
3000772,PRJNA488043_S_NODE_1363_length_165_cov_3.977941_g1352_i0,PRJNA488043,1363,165,3.977941,6.56360265,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,294,2.08e-75,,NTclustered,gi|11466174|ref|NC_002573.1|,5762,g1352,i0,98.788,12.9878787878788,165,2,100,0,1,165,1139,975,"Naegleria gruberi mitochondrion, complete genome",blastn,2143,294,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
4277081,PRJNA488043_S_NODE_1184_length_177_cov_2.898649_g1173_i0,PRJNA488043,1184,177,2.898649,5.13060873,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,294,2.25e-75,,NTclustered,gi|290991714|ref|XM_002678434.1|,5762,g1173,i0,96.61,86.7796610169491,177,6,100,0,1,177,727,551,"Naegleria gruberi uncharacterized protein (NAEGRDRAFT_56113), mRNA",blastn,15360,294,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
4277089,PRJNA488043_S_NODE_464_length_273_cov_12.606557_g453_i0,PRJNA488043,464,273,12.606557,34.41590061,Naegleria jadini,51636,Heterolobosea,other_Eukaryota,505,1.6e-138,,NTclustered,gi|2777861737|gb|PQ072231.1|,51636,g453,i0,100,100.007326007326,273,0,100,0,1,273,1185,913,"Naegleria jadini strain CCAP_1518_2_consensus_sequence small subunit ribosomal RNA gene, partial sequence",blastn,27302,505,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria jadini
4926191,PRJNA488043_S_NODE_484_length_269_cov_5.675000_g473_i0,PRJNA488043,484,269,5.675,15.26575,Naegleria,5761,Heterolobosea,other_Eukaryota,278,3.73e-70,,NTclustered,gi|2618963742|gb|OR769034.1|,5762,g473,i0,99.346,51.3828996282528,153,1,57,0,1,153,3845,3997,"Naegleria gruberi strain NEG-M rDNA-containing extrachromosomal element genomic sequence; and 18S small subunit ribosomal RNA gene, internal transcribed spacer 1, 5.8S ribosomal RNA gene, internal transcribed spacer 2, and 28S large subunit ribosomal RNA gene, complete sequence",blastn,13822,278,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
6827595,PRJNA488043_S_NODE_226_length_366_cov_68.364985_g215_i0,PRJNA488043,226,366,68.364985,250.2158451,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,616,9.829999999999999e-172,,NTclustered,gi|11466174|ref|NC_002573.1|,5762,g215,i0,96.995,101.204918032787,366,11,100,0,1,366,2125,2490,"Naegleria gruberi mitochondrion, complete genome",blastn,37041,616,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
6827603,PRJNA488043_S_NODE_886_length_200_cov_13.707602_g875_i0,PRJNA488043,886,200,13.707602,27.415204,Neobodo designis,312471,Euglenozoa,other_Eukaryota,313,7.22e-81,,NTclustered,gi|197723537|gb|EF681922.2|,312471,g875,i0,95,151.325,200,9,100,1,1,200,1027,1225,"Neobodo designis strain HFCC97 28S ribosomal RNA gene, partial sequence",blastn,30265,313,1,Eukaryota,Discoba,,Euglenozoa,Kinetoplastea,Neobodonida,,Neobodo,Neobodo designis
8102906,PRJNA488043_P_NODE_14867_length_230_cov_1.034826_g12625_i0,PRJNA488043,14867,230,1.034826,2.3800998,Allovahlkampfia sp. F11,1792819,Heterolobosea,other_Eukaryota,124,4.29e-24,,NTclustered,gi|1072301504|dbj|LC106122.1|,1792819,g12625,i0,98.592,13.1391304347826,71,0,69,1,1,70,21,91,"Allovahlkampfia sp. F11 gene for 18S ribosomal RNA, partial sequence",blastn,3022,124,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Allovahlkampfia,Allovahlkampfia sp. F11
8103139,PRJNA488043_S_NODE_107_length_446_cov_15.110312_g96_i0,PRJNA488043,107,446,15.110312,67.39199152,Trypanosoma theileri,67003,Euglenozoa,other_Eukaryota,610,5.679999999999997e-170,,NTclustered,gi|2026667530|dbj|LC618030.1|,67003,g96,i0,91.556,148.208520179372,450,27,99,9,7,446,4803,4355,"Trypanosoma theileri YMG-11 genes for 18S rRNA, ITS1, 5.8S rRNA, ITS2, 28S rRNA, ITS3, ITS4, ITS5, partial and complete sequence",blastn,66101,610,1,Eukaryota,Discoba,,Euglenozoa,Kinetoplastea,Trypanosomatida,Trypanosomatidae,Trypanosoma,Trypanosoma theileri
8751015,PRJNA488043_S_NODE_287_length_331_cov_10.493377_g276_i0,PRJNA488043,287,331,10.493377,34.73307787,Naegleria,5761,Heterolobosea,other_Eukaryota,595,1.1499999999999998e-165,,NTclustered,gi|2618963742|gb|OR769034.1|,5762,g276,i0,99.094,24.9728096676737,331,3,100,0,1,331,3845,4175,"Naegleria gruberi strain NEG-M rDNA-containing extrachromosomal element genomic sequence; and 18S small subunit ribosomal RNA gene, internal transcribed spacer 1, 5.8S ribosomal RNA gene, internal transcribed spacer 2, and 28S large subunit ribosomal RNA gene, complete sequence",blastn,8266,595,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
10026353,PRJNA488043_S_NODE_568_length_247_cov_11.307339_g557_i0,PRJNA488043,568,247,11.307339,27.92912733,Naegleria,5761,Heterolobosea,other_Eukaryota,455,1.4600000000000002e-123,,NTclustered,gi|38606344|gb|AY376152.1|,51634,g557,i0,100,22.3481781376518,246,0,99,0,1,246,189,434,"Naegleria australiensis isolate 7857 small subunit ribosomal RNA gene, partial sequence; mitochondrial",blastn,5520,455,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria australiensis
12577040,PRJNA488043_S_NODE_1010_length_189_cov_9.400000_g999_i0,PRJNA488043,1010,189,9.4,17.766,Naegleria,5761,Heterolobosea,other_Eukaryota,333,5.169999999999999e-87,,NTclustered,gi|38606345|gb|AY376153.1|,5762,g999,i0,98.413,25.2380952380952,189,3,100,0,1,189,694,506,"Naegleria gruberi isolate 7860 small subunit ribosomal RNA gene, partial sequence; mitochondrial",blastn,4770,333,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
13203669,PRJNA488043_S_NODE_1651_length_150_cov_5.338843_g1640_i0,PRJNA488043,1651,150,5.338843,8.0082645,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,257,2.41e-64,,NTclustered,gi|290974732|ref|XM_002670053.1|,5762,g1640,i0,97.973,99.4933333333333,148,3,99,0,1,148,221,368,"Naegleria gruberi uncharacterized protein (NAEGRDRAFT_55502), mRNA",blastn,14924,257,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
14478316,PRJNA488043_P_NODE_11292_length_300_cov_2.966790_g9051_i0,PRJNA488043,11292,300,2.96679,8.90037,Allovahlkampfia sp.,1874883,Heterolobosea,other_Eukaryota,411,3.9999999999999996e-110,,NTclustered,gi|2651357779|gb|PP174295.1|,1874883,g9051,i0,92.388,9.47,289,21,96,1,1,289,2426,2139,"Allovahlkampfia sp. isolate P10 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence",blastn,2841,411,1,Eukaryota,Discoba,,Heterolobosea,,,Acrasidae,Allovahlkampfia,Allovahlkampfia sp.
14478472,PRJNA488043_P_NODE_21692_length_156_cov_11.590551_g19450_i0,PRJNA488043,21692,156,11.590551,18.08125956,Naegleria fowleri,5763,Heterolobosea,other_Eukaryota,172,9.45e-39,,NTclustered,gi|2031941822|emb|OD957988.1|,5763,g19450,i0,93.162,3.3974358974359,117,8,75,0,3,119,181,297,Naegleria fowleri Karachi_NF001 strain,blastn,530,172,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria fowleri
14478593,PRJNA488043_S_NODE_1212_length_175_cov_3.863014_g1201_i0,PRJNA488043,1212,175,3.863014,6.7602745,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,313,6.13e-81,,NTclustered,gi|291000815|ref|XM_002682929.1|,5762,g1201,i0,98.857,64.7485714285714,175,2,100,0,1,175,951,1125,"Naegleria gruberi uncharacterized protein (NAEGRDRAFT_56672), mRNA",blastn,11331,313,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
14478604,PRJNA488043_S_NODE_252_length_349_cov_4.250000_g241_i0,PRJNA488043,252,349,4.25,14.8325,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,599,9.389999999999997e-167,,NTclustered,gi|11466174|ref|NC_002573.1|,5762,g241,i0,97.714,1.00286532951289,350,5,100,3,1,349,270,617,"Naegleria gruberi mitochondrion, complete genome",blastn,350,599,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
15125992,PRJNA488043_P_NODE_5532_length_555_cov_4.186312_g3558_i0,PRJNA488043,5532,555,4.186312,23.2340316,Naegleria,5761,Heterolobosea,other_Eukaryota,1022,0,,NTclustered,gi|2651357780|gb|PP174296.1|,36333,g3558,i0,100,38.1585585585586,553,0,99,0,1,553,1834,2386,"Naegleria clarki isolate P2881 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence",blastn,21178,1022,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria clarki
15752708,PRJNA488043_S_NODE_333_length_310_cov_11.128114_g322_i0,PRJNA488043,333,310,11.128114,34.4971534,Trypanosoma theileri,67003,Euglenozoa,other_Eukaryota,475,1.4499999999999998e-129,,NTclustered,gi|2026667531|dbj|LC618031.1|,67003,g322,i0,94.249,161.383870967742,313,14,100,4,1,310,4364,4675,"Trypanosoma theileri OSK-16 genes for 18S rRNA, ITS1, 5.8S rRNA, ITS2, 28S rRNA, ITS3, ITS4, partial and complete sequence",blastn,50029,475,1,Eukaryota,Discoba,,Euglenozoa,Kinetoplastea,Trypanosomatida,Trypanosomatidae,Trypanosoma,Trypanosoma theileri
15752715,PRJNA488043_S_NODE_773_length_213_cov_2.923913_g762_i0,PRJNA488043,773,213,2.923913,6.22793469,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,364,2.119999999999999e-96,,NTclustered,gi|290971318|ref|XM_002668414.1|,5762,g762,i0,97.642,45.4788732394366,212,5,99,0,2,213,625,414,"Naegleria gruberi uncharacterized protein (NAEGRDRAFT_61055), partial mRNA",blastn,9687,364,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
16400614,PRJNA488043_S_NODE_1533_length_156_cov_10.566929_g1522_i0,PRJNA488043,1533,156,10.566929,16.48440924,Trypanosoma,5690,Euglenozoa,other_Eukaryota,215,1.55e-51,,NTclustered,gi|162233|gb|M63699.1|TRBRGS1,5693,g1522,i0,91.667,120.038461538462,156,12,99,1,2,156,109,264,T.cruzi S3 ribosomal RNA gene,blastn,18726,215,1,Eukaryota,Discoba,,Euglenozoa,Kinetoplastea,Trypanosomatida,Trypanosomatidae,Trypanosoma,Trypanosoma cruzi
17027322,PRJNA488043_S_NODE_814_length_208_cov_5.558659_g803_i0,PRJNA488043,814,208,5.558659,11.56201072,Naegleria australiensis,51634,Heterolobosea,other_Eukaryota,374,3.4199999999999996e-99,,NTclustered,gi|38606344|gb|AY376152.1|,51634,g803,i0,99.038,38.1490384615385,208,2,100,0,1,208,1428,1221,"Naegleria australiensis isolate 7857 small subunit ribosomal RNA gene, partial sequence; mitochondrial",blastn,7935,374,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria australiensis
17675546,PRJNA488043_P_NODE_16314_length_210_cov_2.149171_g14072_i0,PRJNA488043,16314,210,2.149171,4.5132591,Naegleria,5761,Heterolobosea,other_Eukaryota,287,4.64e-73,,NTclustered,gi|2618963742|gb|OR769034.1|,5762,g14072,i0,90.517,10.6571428571429,232,0,100,1,1,210,5569,5800,"Naegleria gruberi strain NEG-M rDNA-containing extrachromosomal element genomic sequence; and 18S small subunit ribosomal RNA gene, internal transcribed spacer 1, 5.8S ribosomal RNA gene, internal transcribed spacer 2, and 28S large subunit ribosomal RNA gene, complete sequence",blastn,2238,287,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
18301660,PRJNA488043_S_NODE_1515_length_157_cov_8.640625_g1504_i0,PRJNA488043,1515,157,8.640625,13.56578125,Naegleria jadini,51636,Heterolobosea,other_Eukaryota,291,2.52e-74,,NTclustered,gi|2777861737|gb|PQ072231.1|,51636,g1504,i0,100,99.2484076433121,157,0,100,0,1,157,918,762,"Naegleria jadini strain CCAP_1518_2_consensus_sequence small subunit ribosomal RNA gene, partial sequence",blastn,15582,291,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria jadini
19575813,PRJNA488043_S_NODE_1636_length_151_cov_2.622951_g1625_i0,PRJNA488043,1636,151,2.622951,3.96065601,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,244,1.89e-60,,NTclustered,gi|290979343|ref|XM_002672348.1|,5762,g1625,i0,96,3.37748344370861,150,6,99,0,1,150,359,508,"Naegleria gruberi uncharacterized protein (NAEGRDRAFT_56312), mRNA",blastn,510,244,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
20851678,PRJNA488043_S_NODE_77_length_505_cov_38.432773_g66_i0,PRJNA488043,77,505,38.432773,194.08550365,Naegleria australiensis,51634,Heterolobosea,other_Eukaryota,898,0,,NTclustered,gi|2476253407|gb|OQ627480.1|,51634,g66,i0,98.81,38.9841584158416,504,6,99,0,2,505,2935,2432,"Naegleria australiensis strain PP397 rDNA-containing extrachromosomal element genomic sequence; small subunit ribosomal RNA gene, internal transcribed spacer 1, 5.8S ribosomal RNA gene, internal transcribed spacer 2, and large subunit ribosomal RNA gene, complete sequence; and non-ribosomal sequence",blastn,19687,898,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria australiensis
22126482,PRJNA488043_S_NODE_1278_length_171_cov_5.253521_g1267_i0,PRJNA488043,1278,171,5.253521,8.98352091,Naegleria jadini,51636,Heterolobosea,other_Eukaryota,316,4.6e-82,,NTclustered,gi|2777861737|gb|PQ072231.1|,51636,g1267,i0,100,99.9883040935673,171,0,100,0,1,171,101,271,"Naegleria jadini strain CCAP_1518_2_consensus_sequence small subunit ribosomal RNA gene, partial sequence",blastn,17098,316,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria jadini
22775066,PRJNA488043_S_NODE_1018_length_188_cov_34.320755_g1007_i0,PRJNA488043,1018,188,34.320755,64.5230194,Trypanosomatidae,5654,Euglenozoa,other_Eukaryota,246,6.889999999999999e-61,,NTclustered,gi|2414483|emb|Y00055.1|,5656,g1007,i0,90.426,121.542553191489,188,17,100,1,1,188,6845,7031,Crithidia fasciculata ribosomal DNA repeat (complete),blastn,22850,248,0.991935483870968,Eukaryota,Discoba,,Euglenozoa,Kinetoplastea,Trypanosomatida,Trypanosomatidae,Crithidia,Crithidia fasciculata
23402331,PRJNA488043_S_NODE_679_length_225_cov_9.326531_g668_i0,PRJNA488043,679,225,9.326531,20.98469475,Naegleria gruberi,5762,Heterolobosea,other_Eukaryota,405,1.33e-108,,NTclustered,gi|11466174|ref|NC_002573.1|,5762,g668,i0,99.111,15.04,225,2,100,0,1,225,1955,1731,"Naegleria gruberi mitochondrion, complete genome",blastn,3384,405,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria gruberi
24049952,PRJNA488043_S_NODE_1379_length_164_cov_6.940741_g1368_i0,PRJNA488043,1379,164,6.940741,11.38281524,Naegleria,5761,Heterolobosea,other_Eukaryota,292,7.41e-75,,NTclustered,gi|2476253407|gb|OQ627480.1|,51634,g1368,i0,98.78,12,164,2,100,0,1,164,4136,4299,"Naegleria australiensis strain PP397 rDNA-containing extrachromosomal element genomic sequence; small subunit ribosomal RNA gene, internal transcribed spacer 1, 5.8S ribosomal RNA gene, internal transcribed spacer 2, and large subunit ribosomal RNA gene, complete sequence; and non-ribosomal sequence",blastn,1968,292,1,Eukaryota,Discoba,,Heterolobosea,,,Vahlkampfiidae,Naegleria,Naegleria australiensis