run_metadata
27 rows where technology = "unknown", tissue_curation = "Thymus" and tissue_curation_coarse = "Hematopoietic System"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 59515 | 59515 | SRR11926689 | SRX8472335 | SRS6772936 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | WT5 | GSM4591370 | tissue:Healthy sorted thymus|cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | WT5 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy sorted thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | GSM4591370 | GSM4591370: WT5; Danio rerio; RNA Seq | GSM4591370 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591370 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | FWTsample_5.fastq.gz FWTsample_5_R2.fastq.gz | fastq fastq | 4633582858.0 | 22938529.0 | GSM4591370 r1 | 0:101 1:101 | A:1263929939;C:1047240206;G:1052800791;T:1263574247;N:6037675 | 101 | 101 | 1263929939 | 1047240206 | 1052800791 | 1263574247 | 6037675 | SRX8472335 | SRS6772936 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.91347 | 0.91728 | 0.27187 | 0.27409 | 0.76585 | 0.76859 | 0.45662 | 0.48071 | 101 | 101 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59516 | 59516 | SRR11926688 | SRX8472334 | SRS6772935 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | WT4 | GSM4591369 | tissue:Healthy sorted thymus|cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | WT4 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy sorted thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | GSM4591369 | GSM4591369: WT4; Danio rerio; RNA Seq | GSM4591369 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591369 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | FWTsample_4.fastq.gz FWTsample_4_R2.fastq.gz | fastq fastq | 4113149600.0 | 20565748.0 | GSM4591369 r1 | 0:100 1:100 | A:1084628064;C:963840734;G:985823964;T:1078691018;N:165820 | 100 | 100 | 1084628064 | 963840734 | 985823964 | 1078691018 | 165820 | SRX8472334 | SRS6772935 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.9336 | 0.93539 | 0.11919 | 0.11989 | 0.76368 | 0.76757 | 0.4904 | 0.47498 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59517 | 59517 | SRR11926687 | SRX8472333 | SRS6772934 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | WT3 | GSM4591368 | tissue:Healthy sorted thymus|cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | WT3 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy sorted thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | GSM4591368 | GSM4591368: WT3; Danio rerio; RNA Seq | GSM4591368 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591368 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | FWTsample_3_R2.fastq.gz FWTsample_3.fastq.gz | fastq fastq | 3966667200.0 | 19833336.0 | GSM4591368 r1 | 0:100 1:100 | A:1125938234;C:857622800;G:856236104;T:1122434590;N:4435472 | 100 | 100 | 1125938234 | 857622800 | 856236104 | 1122434590 | 4435472 | SRX8472333 | SRS6772934 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.93918 | 0.92495 | 0.16292 | 0.15907 | 0.75866 | 0.76883 | 0.51794 | 0.51278 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59518 | 59518 | SRR11926686 | SRX8472332 | SRS6772933 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | WT2 | GSM4591367 | tissue:Healthy sorted thymus|cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | WT2 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy sorted thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | GSM4591367 | GSM4591367: WT2; Danio rerio; RNA Seq | GSM4591367 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591367 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | FWTsample_2_R2.fastq.gz FWTsample_2.fastq.gz | fastq fastq | 3598961400.0 | 17994807.0 | GSM4591367 r1 | 0:100 1:100 | A:982353707;C:818188790;G:810911943;T:983465623;N:4041337 | 100 | 100 | 982353707 | 818188790 | 810911943 | 983465623 | 4041337 | SRX8472332 | SRS6772933 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.93426 | 0.9195 | 0.12591 | 0.12501 | 0.76763 | 0.77621 | 0.49491 | 0.50063 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59519 | 59519 | SRR11926685 | SRX8472331 | SRS6772932 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | WT1 | GSM4591366 | tissue:Healthy sorted thymus|cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | WT1 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy sorted thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tglck:GFP|tumor stg:healthy|lab of sample collection and sequencing:Frazer lab | GSM4591366 | GSM4591366: WT1; Danio rerio; RNA Seq | GSM4591366 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591366 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | FWTsample_1_R2.fastq.gz FWTsample_1.fastq.gz | fastq fastq | 3686706000.0 | 18433530.0 | GSM4591366 r1 | 0:100 1:100 | A:1004954280;C:838347690;G:833756041;T:1005493425;N:4154564 | 100 | 100 | 1004954280 | 838347690 | 833756041 | 1005493425 | 4154564 | SRX8472331 | SRS6772932 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.93171 | 0.91783 | 0.13677 | 0.13606 | 0.76723 | 0.7752 | 0.51062 | 0.50949 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59520 | 59520 | SRR11926684 | SRX8472330 | SRS6772931 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | SPL002 | GSM4591365 | tissue:shrek preleukemic thymocytes|cell type:thymocytes|genotype:shrek;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | SPL002 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | shrek preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:shrek;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591365 | GSM4591365: SPL002; Danio rerio; RNA Seq | GSM4591365 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591365 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_48.fastq.gz Fsample_48_R2.fastq.gz | fastq fastq | 23525993582.0 | 77900641.0 | GSM4591365 r1 | 0:151 1:151 | A:6365057472;C:5454588670;G:5620997543;T:6082859005;N:2490892 | 151 | 151 | 6365057472 | 5454588670 | 5620997543 | 6082859005 | 2490892 | SRX8472330 | SRS6772931 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.96011 | 0.96113 | 0.23076 | 0.22681 | 0.80858 | 0.81221 | 0.52577 | 0.51928 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59521 | 59521 | SRR11926683 | SRX8472329 | SRS6772930 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | SPL001 | GSM4591364 | tissue:shrek preleukemic thymocytes|cell type:thymocytes|genotype:shrek;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | SPL001 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | shrek preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:shrek;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591364 | GSM4591364: SPL001; Danio rerio; RNA Seq | GSM4591364 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591364 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_47.fastq.gz Fsample_47_R2.fastq.gz | fastq fastq | 26795594770.0 | 88727135.0 | GSM4591364 r1 | 0:151 1:151 | A:7305822132;C:6082789601;G:6442162847;T:6961938191;N:2881999 | 151 | 151 | 7305822132 | 6082789601 | 6442162847 | 6961938191 | 2881999 | SRX8472329 | SRS6772930 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.96139 | 0.96343 | 0.22215 | 0.2185 | 0.81296 | 0.81647 | 0.50868 | 0.51446 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59532 | 59532 | SRR11926672 | SRX8472318 | SRS6772919 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | OPL4 | GSM4591353 | tissue:otg preleukemic thymocytes|cell type:thymocytes|genotype:otg;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | OPL4 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | otg preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:otg;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591353 | GSM4591353: OPL4; Danio rerio; RNA Seq | GSM4591353 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591353 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_36.fastq.gz Fsample_36_R2.fastq.gz | fastq fastq | 4686865610.0 | 23202305.0 | GSM4591353 r1 | 0:101 1:101 | A:1290395271;C:1046629683;G:1049444449;T:1294288823;N:6107384 | 101 | 101 | 1290395271 | 1046629683 | 1049444449 | 1294288823 | 6107384 | SRX8472318 | SRS6772919 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.92236 | 0.92454 | 0.31359 | 0.31617 | 0.76625 | 0.76779 | 0.48273 | 0.47205 | 101 | 101 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59533 | 59533 | SRR11926671 | SRX8472317 | SRS6772918 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | OPL3 | GSM4591352 | tissue:otg preleukemic thymocytes|cell type:thymocytes|genotype:otg;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | OPL3 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | otg preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:otg;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591352 | GSM4591352: OPL3; Danio rerio; RNA Seq | GSM4591352 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591352 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_35_R2.fastq.gz Fsample_35.fastq.gz | fastq fastq | 6253893538.0 | 30959869.0 | GSM4591352 r1 | 0:101 1:101 | A:1720308233;C:1397371474;G:1404797248;T:1723210472;N:8206111 | 101 | 101 | 1720308233 | 1397371474 | 1404797248 | 1723210472 | 8206111 | SRX8472317 | SRS6772918 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.93192 | 0.93318 | 0.35833 | 0.3632 | 0.78518 | 0.78788 | 0.48278 | 0.48009 | 101 | 101 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59534 | 59534 | SRR11926670 | SRX8472316 | SRS6772917 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | OPL2 | GSM4591351 | tissue:otg preleukemic thymocytes|cell type:thymocytes|genotype:otg;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | OPL2 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | otg preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:otg;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591351 | GSM4591351: OPL2; Danio rerio; RNA Seq | GSM4591351 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591351 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_34_R2.fastq.gz Fsample_34.fastq.gz | fastq fastq | 5351903948.0 | 26494574.0 | GSM4591351 r1 | 0:101 1:101 | A:1442793834;C:1227325750;G:1233975738;T:1440891564;N:6917062 | 101 | 101 | 1442793834 | 1227325750 | 1233975738 | 1440891564 | 6917062 | SRX8472316 | SRS6772917 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.92964 | 0.93101 | 0.21706 | 0.2185 | 0.76497 | 0.76848 | 0.47634 | 0.48247 | 101 | 101 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59535 | 59535 | SRR11926669 | SRX8472315 | SRS6772916 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | OPL1 | GSM4591350 | tissue:otg preleukemic thymocytes|cell type:thymocytes|genotype:otg;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | OPL1 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | otg preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:otg;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591350 | GSM4591350: OPL1; Danio rerio; RNA Seq | GSM4591350 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591350 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_33.fastq.gz Fsample_33_R2.fastq.gz | fastq fastq | 5499039536.0 | 27222968.0 | GSM4591350 r1 | 0:101 1:101 | A:1515036859;C:1226882670;G:1229198085;T:1520688016;N:7233906 | 101 | 101 | 1515036859 | 1226882670 | 1229198085 | 1520688016 | 7233906 | SRX8472315 | SRS6772916 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.92637 | 0.92762 | 0.31857 | 0.32243 | 0.76769 | 0.77116 | 0.46456 | 0.46584 | 101 | 101 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59546 | 59546 | SRR11926658 | SRX8472304 | SRS6772905 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | MPL3 | GSM4591339 | tissue:hMYC preleukemic thymocytes|cell type:thymocytes|genotype:Tgrag2:hMYC Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | MPL3 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | hMYC preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:hMYC Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591339 | GSM4591339: MPL3; Danio rerio; RNA Seq | GSM4591339 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591339 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_21.fastq.gz Fsample_21_R2.fastq.gz | fastq fastq | 8876009218.0 | 29390759.0 | GSM4591339 r1 | 0:151 1:151 | A:2397445782;C:2052027740;G:2018874313;T:2405562242;N:2099141 | 151 | 151 | 2397445782 | 2052027740 | 2018874313 | 2405562242 | 2099141 | SRX8472304 | SRS6772905 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.92414 | 0.9227 | 0.2461 | 0.23997 | 0.78841 | 0.79506 | 0.50203 | 0.5018 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59547 | 59547 | SRR11926657 | SRX8472303 | SRS6772904 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | MPL2 | GSM4591338 | tissue:hMYC preleukemic thymocytes|cell type:thymocytes|genotype:Tgrag2:hMYC Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | MPL2 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | hMYC preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:hMYC Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591338 | GSM4591338: MPL2; Danio rerio; RNA Seq | GSM4591338 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591338 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_20.fastq.gz Fsample_20_R2.fastq.gz | fastq fastq | 8942441970.0 | 29610735.0 | GSM4591338 r1 | 0:151 1:151 | A:2434196467;C:2047066969;G:2021293350;T:2437752994;N:2132190 | 151 | 151 | 2434196467 | 2047066969 | 2021293350 | 2437752994 | 2132190 | SRX8472303 | SRS6772904 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.9147 | 0.91499 | 0.22028 | 0.2117 | 0.77713 | 0.78334 | 0.50243 | 0.49838 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59548 | 59548 | SRR11926656 | SRX8472302 | SRS6772903 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | MPL1 | GSM4591337 | tissue:hMYC preleukemic thymocytes|cell type:thymocytes|genotype:Tgrag2:hMYC Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | MPL1 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | hMYC preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:hMYC Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591337 | GSM4591337: MPL1; Danio rerio; RNA Seq | GSM4591337 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591337 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_19.fastq.gz Fsample_19_R2.fastq.gz | fastq fastq | 9778540144.0 | 32379272.0 | GSM4591337 r1 | 0:151 1:151 | A:2676504038;C:2223347787;G:2177506547;T:2698864055;N:2317717 | 151 | 151 | 2676504038 | 2223347787 | 2177506547 | 2698864055 | 2317717 | SRX8472302 | SRS6772903 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.91346 | 0.91413 | 0.29555 | 0.28893 | 0.79078 | 0.79825 | 0.49875 | 0.50518 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59549 | 59549 | SRR11926655 | SRX8472301 | SRS6772902 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | HPL3 | GSM4591336 | tissue:hulk mutant preleukemic thymocytes|cell type:thymocytes|genotype:hulk;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | HPL3 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | hulk mutant preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:hulk;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591336 | GSM4591336: HPL3; Danio rerio; RNA Seq | GSM4591336 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591336 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_12.fastq.gz Fsample_12_R2.fastq.gz | fastq fastq | 3378760800.0 | 16893804.0 | GSM4591336 r1 | 0:100 1:100 | A:869653801;C:820588210;G:823025653;T:861750769;N:3742367 | 100 | 100 | 869653801 | 820588210 | 823025653 | 861750769 | 3742367 | SRX8472301 | SRS6772902 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.93754 | 0.92151 | 0.17513 | 0.16969 | 0.79922 | 0.8071 | 0.63894 | 0.61913 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59550 | 59550 | SRR11926654 | SRX8472300 | SRS6772901 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | HPL2 | GSM4591335 | tissue:hulk mutant preleukemic thymocytes|cell type:thymocytes|genotype:hulk;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | HPL2 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | hulk mutant preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:hulk;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591335 | GSM4591335: HPL2; Danio rerio; RNA Seq | GSM4591335 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591335 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_11.fastq.gz Fsample_11_R2.fastq.gz | fastq fastq | 3276290400.0 | 16381452.0 | GSM4591335 r1 | 0:100 1:100 | A:909076954;C:730010076;G:728583319;T:904911154;N:3708897 | 100 | 100 | 909076954 | 730010076 | 728583319 | 904911154 | 3708897 | SRX8472300 | SRS6772901 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.93409 | 0.92023 | 0.18134 | 0.18141 | 0.78108 | 0.78981 | 0.53659 | 0.52431 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59551 | 59551 | SRR11926653 | SRX8472299 | SRS6772900 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | HPL1 | GSM4591334 | tissue:hulk mutant preleukemic thymocytes|cell type:thymocytes|genotype:hulk;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | HPL1 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | hulk mutant preleukemic thymocytes | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:hulk;Tglck:GFP|tumor stg:preleukemic|lab of sample collection and sequencing:Frazer lab | GSM4591334 | GSM4591334: HPL1; Danio rerio; RNA Seq | GSM4591334 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591334 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 3000 | SRP265974 | Fsample_10.fastq.gz Fsample_10_R2.fastq.gz | fastq fastq | 3700727800.0 | 18503639.0 | GSM4591334 r1 | 0:100 1:100 | A:1028412839;C:821049620;G:815741395;T:1031399520;N:4124426 | 100 | 100 | 1028412839 | 821049620 | 815741395 | 1031399520 | 4124426 | SRX8472299 | SRS6772900 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 2 | 0.93381 | 0.92058 | 0.22321 | 0.22223 | 0.73655 | 0.74732 | 0.4822 | 0.48829 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||
| 59579 | 59579 | SRR11926625 | SRX8472271 | SRS6772872 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | AB thymus 9 | GSM4591306 | tissue:Healthy dissected thymus|cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | AB thymus 9 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy dissected thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | GSM4591306 | GSM4591306: AB thymus 9; Danio rerio; RNA Seq | GSM4591306 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591306 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | NextSeq 500 | SRP265974 | sample_6.fastq.gz | fastq | 2786385619.0 | 36888152.0 | GSM4591306 r1 | 0:75.54 1:0 | A:663089588;C:692060734;G:651825670;T:779197417;N:212210 | 75 | 0 | 663089588 | 692060734 | 651825670 | 779197417 | 212210 | SRX8472271 | SRS6772872 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 1 | 0.94942 | 0.05717 | 0.73018 | 0.49009 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||||||||
| 59580 | 59580 | SRR11926624 | SRX8472270 | SRS6772871 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | AB thymus 8 | GSM4591305 | tissue:Healthy dissected thymus|cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | AB thymus 8 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy dissected thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | GSM4591305 | GSM4591305: AB thymus 8; Danio rerio; RNA Seq | GSM4591305 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591305 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | NextSeq 500 | SRP265974 | sample_5.fastq.gz | fastq | 2456067469.0 | 32519095.0 | GSM4591305 r1 | 0:75.53 1:0 | A:606304745;C:594426559;G:556316196;T:698842180;N:177789 | 75 | 0 | 606304745 | 594426559 | 556316196 | 698842180 | 177789 | SRX8472270 | SRS6772871 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 1 | 0.94282 | 0.08874 | 0.70851 | 0.49253 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||||||||
| 59581 | 59581 | SRR11926623 | SRX8472269 | SRS6772870 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | AB thymus 7 | GSM4591304 | tissue:Healthy dissected thymus|cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | AB thymus 7 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy dissected thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | GSM4591304 | GSM4591304: AB thymus 7; Danio rerio; RNA Seq | GSM4591304 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591304 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | NextSeq 500 | SRP265974 | sample_4.fastq.gz | fastq | 2889915115.0 | 38259114.0 | GSM4591304 r1 | 0:75.54 1:0 | A:694082728;C:713330573;G:669832103;T:812454853;N:214858 | 75 | 0 | 694082728 | 713330573 | 669832103 | 812454853 | 214858 | SRX8472269 | SRS6772870 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 1 | 0.94794 | 0.06446 | 0.72697 | 0.49962 | 75 | B | usable mapping rate | illumina | nextseq | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||||||||
| 59582 | 59582 | SRR11926622 | SRX8472268 | SRS6772869 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | AB thymus 2 3 5 6 | GSM4591303 | tissue:Healthy dissected thymus|cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | AB thymus 2 3 5 6 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy dissected thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | GSM4591303 | GSM4591303: AB thymus 2 3 5 6; Danio rerio; RNA Seq | GSM4591303 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591303 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | NextSeq 500 | SRP265974 | sample_3.fastq.gz | fastq | 2338535479.0 | 30955998.0 | GSM4591303 r1 | 0:75.54 1:0 | A:540165709;C:595429439;G:553807340;T:648952381;N:180610 | 75 | 0 | 540165709 | 595429439 | 553807340 | 648952381 | 180610 | SRX8472268 | SRS6772869 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 1 | 0.94992 | 0.04962 | 0.73032 | 0.4837 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||||||||
| 59583 | 59583 | SRR11926621 | SRX8472267 | SRS6772868 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | AB thymus 11 | GSM4591302 | tissue:Healthy dissected thymus|cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | AB thymus 11 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy dissected thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | GSM4591302 | GSM4591302: AB thymus 11; Danio rerio; RNA Seq | GSM4591302 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591302 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | NextSeq 500 | SRP265974 | sample_2.fastq.gz | fastq | 2942053849.0 | 38949737.0 | GSM4591302 r1 | 0:75.53 1:0 | A:688398915;C:734856328;G:694653408;T:823920342;N:224856 | 75 | 0 | 688398915 | 734856328 | 694653408 | 823920342 | 224856 | SRX8472267 | SRS6772868 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 1 | 0.94862 | 0.06975 | 0.72224 | 0.49305 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||||||||
| 59584 | 59584 | SRR11926620 | SRX8472266 | SRS6772867 | SRP265974 | PRJNA637328 | A novel TLX1 driven T ALL zebrafish model: comparative genomic analysis with other leukemia models | GSE151816 | Transcriptome Analysis | We generated a new rag2 TLX1 driven T ALL model in zebrafish. RNA sequencing was preformed on the developed TLX1 T ALLs sequencing performed in the Speleman lab. In addition RNA sequencing was performed on various additional zebrafish T ALL models shrek hulk otg hMYC sequenced in the Frazer lab to look at the molecular differences between the various existing T ALL models. Overall design: RNA sequencing data from TLX1 shrek hulk otg T ALL samples wild type thymocytes sequenced in the Speleman lab wild type thymocytes sequenced in the Frazer lab. Shrek otg hulk hMYC preleukemic thymocytes | pubmed:32591643 | AB thymus 10 | GSM4591301 | tissue:Healthy dissected thymus|cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | AB thymus 10 | First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome build: GRCz10 Supplementary files format and content: txt file with raw counts | Healthy dissected thymus | Truseq stranded mRNA library prep illumina #20020594 | cell type:thymocytes|genotype:Tgrag2:GFP|tumor stg:healthy|lab of sample collection and sequencing:Speleman lab | GSM4591301 | GSM4591301: AB thymus 10; Danio rerio; RNA Seq | GSM4591301 | 1 | Truseq stranded mRNA library prep illumina #20020594 | GEO Accession:GSM4591301 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | NextSeq 500 | SRP265974 | sample_1.fastq.gz | fastq | 2923525817.0 | 38707354.0 | GSM4591301 r1 | 0:75.53 1:0 | A:697204107;C:721945575;G:689048693;T:815100976;N:226466 | 75 | 0 | 697204107 | 721945575 | 689048693 | 815100976 | 226466 | SRX8472266 | SRS6772867 | SRA1083220 | GEO | Center for Medical Genetics, Ghent University | 1 | 0.94964 | 0.05377 | 0.71904 | 0.4902 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | Belgium | 2020-06-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | ||||||||||||||||||
| 68889 | 68889 | SRR18218067 | SRX14364507 | SRS12177802 | SRP362416 | PRJNA812715 | Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia | PRJNA812715 | Other | T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential. | Thy rag2 RFP 6 | strain:Tu/AB|age:not collected|sex:not collected|tissue:thymus|birth date: |death date:02 07 2019|genotype:rag2:RFP|biological replicate:Thy rag2 RFP biological replicate 4|BioSampleModel:Model organism or animal | RNA seq of Danio rerio thymus Tu/AB | Thy rag2 RFP 6 | Thy rag2 RFP 6 | Tu/AB WT zebrafish were sacrificed to harvest the thymus visible through RFP staining for further analysis. The RNA was extracted from sorted thymic cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000. | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP362416 | FCHT5GHDSXX_L1_HKRDZEBmfpEAAPRABPEI-P86H2_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAPRABPEI-P86H2_2.fq.gz | fastq fastq | 18061636200.0 | 60205454.0 | FCHT5GHDSXX L1 HKRDZEBmfpEAAPRABPEI P86H2 1.fq.gz | 0:150 1:150 | A:4749806271;C:4292116510;G:4365562558;T:4654124713;N:26148 | 150 | 150 | 4749806271 | 4292116510 | 4365562558 | 4654124713 | 26148 | SRX14364507 | SRS12177802 | SRA1380603 | Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab | Instituto de Medicina Molecular Joao Lobo Antunes | 2 | 0.9283 | 0.92884 | 0.11636 | 0.1154 | 0.77642 | 0.77697 | 0.48938 | 0.49117 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | Portugal | 2022-03-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | |||||||||||||||||||||
| 68890 | 68890 | SRR18218069 | SRX14364506 | SRS12177801 | SRP362416 | PRJNA812715 | Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia | PRJNA812715 | Other | T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential. | Thy rag2 RFP 5 | strain:Tu/AB|age:not collected|sex:not collected|tissue:thymus|birth date: |death date:02 07 2019|genotype:rag2:RFP|biological replicate:Thy rag2 RFP biological replicate 3|BioSampleModel:Model organism or animal | RNA seq of Danio rerio thymus Tu/AB | Thy rag2 RFP 5 | Thy rag2 RFP 5 | Tu/AB WT zebrafish were sacrificed to harvest the thymus visible through RFP staining for further analysis. The RNA was extracted from sorted thymic cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000. | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP362416 | FCHT5GHDSXX_L1_HKRDZEBmfpEAAORAAPEI-P74G2_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAORAAPEI-P74G2_2.fq.gz | fastq fastq | 16916167800.0 | 56387226.0 | FCHT5GHDSXX L1 HKRDZEBmfpEAAORAAPEI P74G2 1.fq.gz | 0:150 1:150 | A:4469149097;C:4004191756;G:4070955203;T:4371847041;N:24703 | 150 | 150 | 4469149097 | 4004191756 | 4070955203 | 4371847041 | 24703 | SRX14364506 | SRS12177801 | SRA1380603 | Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab | Instituto de Medicina Molecular Joao Lobo Antunes | 2 | 0.92644 | 0.92786 | 0.13071 | 0.13011 | 0.77833 | 0.77792 | 0.47942 | 0.4816 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | Portugal | 2022-03-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | |||||||||||||||||||||
| 68891 | 68891 | SRR18218070 | SRX14364505 | SRS12177800 | SRP362416 | PRJNA812715 | Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia | PRJNA812715 | Other | T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential. | Thy rag2 RFP 4 | strain:Tu/AB|age:not collected|sex:not collected|tissue:thymus|birth date: |death date:02 07 2019|genotype:rag2:RFP|biological replicate:Thy rag2 RFP biological replicate 2|BioSampleModel:Model organism or animal | RNA seq of Danio rerio thymus Tu/AB | Thy rag2 RFP 4 | Thy rag2 RFP 4 | Tu/AB WT zebrafish were sacrificed to harvest the thymus visible through RFP staining for further analysis. The RNA was extracted from sorted thymic cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000. | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP362416 | FCHT5GHDSXX_L1_HKRDZEBmfpEAANRABPEI-P62F2_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAANRABPEI-P62F2_2.fq.gz | fastq fastq | 17996947500.0 | 59989825.0 | FCHT5GHDSXX L1 HKRDZEBmfpEAANRABPEI P62F2 1.fq.gz | 0:150 1:150 | A:4882125302;C:4136332786;G:4194970590;T:4783492952;N:25870 | 150 | 150 | 4882125302 | 4136332786 | 4194970590 | 4783492952 | 25870 | SRX14364505 | SRS12177800 | SRA1380603 | Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab | Instituto de Medicina Molecular Joao Lobo Antunes | 2 | 0.86095 | 0.86165 | 0.25113 | 0.2504 | 0.80204 | 0.80188 | 0.48309 | 0.48627 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | Portugal | 2022-03-04 | Undetermined | Undetermined | Thymus | Hematopoietic System | |||||||||||||||||||||
| 68892 | 68892 | SRR18218071 | SRX14364504 | SRS12177799 | SRP362416 | PRJNA812715 | Mutant IL7R collaborates with MYC to induce T cell Acute Lymphoblastic Leukemia | PRJNA812715 | Other | T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations 10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha encoded by IL7R which occur in different molecular subtypes of this disease. However it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential. | Thy rag2 RFP 3 | strain:Tu/AB|age:not collected|sex:not collected|tissue:thymus|birth date: |death date:02 07 2019|genotype:rag2:RFP|biological replicate:Thy rag2 RFP biological replicate 1|BioSampleModel:Model organism or animal | RNA seq of Danio rerio thymus Tu/AB | Thy rag2 RFP 3 | Thy rag2 RFP 3 | Tu/AB WT zebrafish were sacrificed to harvest the thymus visible through RFP staining for further analysis. The RNA was extracted from sorted thymic cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT fragmented and converted to cDNA size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000. | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP362416 | FCHT5GHDSXX_L1_HKRDZEBmfpEAAMRABPEI-P50E2_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAMRABPEI-P50E2_2.fq.gz | fastq fastq | 18041602200.0 | 60138674.0 | FCHT5GHDSXX L1 HKRDZEBmfpEAAMRABPEI P50E2 1.fq.gz | 0:150 1:150 | A:4726391295;C:4303349397;G:4380843440;T:4630991494;N:26574 | 150 | 150 | 4726391295 | 4303349397 | 4380843440 | 4630991494 | 26574 | SRX14364504 | SRS12177799 | SRA1380603 | Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab | Instituto de Medicina Molecular Joao Lobo Antunes | 2 | 0.93138 | 0.9316 | 0.11291 | 0.11196 | 0.78143 | 0.78261 | 0.4799 | 0.47958 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | Portugal | 2022-03-04 | Undetermined | Undetermined | Thymus | Hematopoietic System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;