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19 rows where experiment.platform = "PACBIO_SMRT" and tissue_curation = "Gonad"

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experiment.library_selection 2

devstage_curation_coarse 2

devstage_curation 2

tissue_curation_coarse 1

tissue_curation 1

  • Gonad · 19 ✖

technology 1

experiment.platform 1

  • PACBIO_SMRT · 19 ✖

experiment.library_strategy 1

experiment.library_source 1

experiment.library_layout 1

Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
34965 34965 SRR32588722 SRX27895229 SRS24266229 SRP568323 PRJNA1232602 Telomere to telomere genome assemblies for commonly used zebrafish laboratory strains PRJNA1232602 Other In this study Iso Seq was performed on different zebrafish body organs enabling a comprehensive view of organ specific transcriptomes. High quality PacBio Iso Seq long reads were generated from key zebrafish tissues including the brain testis liver eye muscle ovary inner ear and kidney to identify novel isoforms tissue specific transcripts and alternative splicing events. We also made available the Iso Seq data from embryos from different time points hpf 0 6 12 and 24. This dataset enhances gene annotation improves reference genome annotations and provides insights into zebrafish organ specific gene expression.       Iso Seq RNA from Danio rerio C2 F2 M T   strain:NHGRI2|isolate:Single paired cross|breed:Zebrafish|cultivar:wild type|ecotype:United States|age:9 month|dev stage:adult fish|collection date:2024 06|geo loc name:USA|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 Iso Seq RNA from testis C2 F2 M T C2 F2 M T The long Read Sequencing libraries was sequenced using PacBio Sequel II     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE PACBIO_SMRT Sequel II   SRP568323     m84270_240911_150713_s1.skera.flnc.fastq.gz fastq 103111938047.0 50624762.0 m84270 240911 150713 s1.skera.flnc.fastq.gz 0:2036.79 A:30106378730;C:21760983790;G:23176083069;T:28068492458;N:0 2036       30106378730 21760983790 23176083069 28068492458 0 SRX27895229 SRS24266229 SRA2089085 National Human Genome Research Institute|Translational and Functional Genomics Branch National Human Genome Research Institute National Human Genome Research Institute                       T   long read pacbio pacbio_modern unknown cdna_unspecified unknown bulk unknown unknown   United States 2025-03-06 Adult Adult Gonad Reproductive System
34974 34974 SRR32588731 SRX27895220 SRS24266226 SRP568323 PRJNA1232602 Telomere to telomere genome assemblies for commonly used zebrafish laboratory strains PRJNA1232602 Other In this study Iso Seq was performed on different zebrafish body organs enabling a comprehensive view of organ specific transcriptomes. High quality PacBio Iso Seq long reads were generated from key zebrafish tissues including the brain testis liver eye muscle ovary inner ear and kidney to identify novel isoforms tissue specific transcripts and alternative splicing events. We also made available the Iso Seq data from embryos from different time points hpf 0 6 12 and 24. This dataset enhances gene annotation improves reference genome annotations and provides insights into zebrafish organ specific gene expression.       Iso Seq RNA from Danio rerio C2 F2 F O32   strain:NHGRI2|isolate:Single paired cross|breed:Zebrafish|cultivar:wild type|ecotype:United States|age:9 month|dev stage:adult fish|collection date:2024 06|geo loc name:USA|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Iso Seq RNA from ovary C2 F2 F O32 C2 F2 F O32 The long Read Sequencing libraries was sequenced using PacBio Sequel II     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE PACBIO_SMRT Sequel II   SRP568323     m64467e_240815_161552.hifi_reads.flnc.fastq.gz fastq 12555748052.0 3350326.0 m64467e 240815 161552.hifi reads.flnc.fastq.gz 0:3747.62 A:3457885256;C:2882310858;G:2971165409;T:3244386529;N:0 3747       3457885256 2882310858 2971165409 3244386529 0 SRX27895220 SRS24266226 SRA2089085 National Human Genome Research Institute|Translational and Functional Genomics Branch National Human Genome Research Institute National Human Genome Research Institute                       T   long read pacbio pacbio_modern unknown cdna_unspecified unknown bulk unknown unknown   United States 2025-03-06 Adult Adult Gonad Reproductive System
34975 34975 SRR32588732 SRX27895219 SRS24266221 SRP568323 PRJNA1232602 Telomere to telomere genome assemblies for commonly used zebrafish laboratory strains PRJNA1232602 Other In this study Iso Seq was performed on different zebrafish body organs enabling a comprehensive view of organ specific transcriptomes. High quality PacBio Iso Seq long reads were generated from key zebrafish tissues including the brain testis liver eye muscle ovary inner ear and kidney to identify novel isoforms tissue specific transcripts and alternative splicing events. We also made available the Iso Seq data from embryos from different time points hpf 0 6 12 and 24. This dataset enhances gene annotation improves reference genome annotations and provides insights into zebrafish organ specific gene expression.       Iso Seq RNA from Danio rerio C2 F2 F O31   strain:NHGRI2|isolate:Single paired cross|breed:Zebrafish|cultivar:wild type|ecotype:United States|age:9 month|dev stage:adult fish|collection date:2024 06|geo loc name:USA|sex:female|tissue:Ovary|BioSampleModel:Model organism or animal                 Iso Seq RNA from ovary C2 F2 F O31 C2 F2 F O31 The long Read Sequencing libraries was sequenced using PacBio Sequel II     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE PACBIO_SMRT Sequel II   SRP568323     m84270_240911_210533_s4.skera.flnc.fastq.gz fastq 91708771607.0 33888263.0 m84270 240911 210533 s4.skera.flnc.fastq.gz 0:2706.21 A:25318206966;C:20635348405;G:21357313254;T:24397902982;N:0 2706       25318206966 20635348405 21357313254 24397902982 0 SRX27895219 SRS24266221 SRA2089085 National Human Genome Research Institute|Translational and Functional Genomics Branch National Human Genome Research Institute National Human Genome Research Institute                       T   long read pacbio pacbio_modern unknown cdna_unspecified unknown bulk unknown unknown   United States 2025-03-06 Adult Adult Gonad Reproductive System
50511 50511 SRR8293425 SRX5108028 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 2 3K D03 1 2 3K D03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160828_125709_42199_c101055502550000001823247601061763_s1_p0.1.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.2.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.3.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 3189114337.0 163482.0 2 3K D03 1.tar.gz   A:1019691460;C:775938787;G:628460211;T:736274173;N:0         1019691460 775938787 628460211 736274173 0 SRX5108028 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50512 50512 SRR8293426 SRX5108027 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 2 3K E03 1 2 3K E03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160828_171546_42199_c101055502550000001823247601061764_s1_p0.1.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.2.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.3.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 3603145484.0 163482.0 2 3K E03 1.tar.gz   A:1041332538;C:789941375;G:785608794;T:952634306;N:0         1041332538 789941375 785608794 952634306 0 SRX5108027 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50513 50513 SRR8293427 SRX5108026 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 1 2K D05 1 1 2K D05 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160831_064528_42199_c101016682550000001823228410211623_s1_p0.1.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.2.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.3.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 4494598972.0 163482.0 1 2K D05 1.tar.gz   A:1263523570;C:919488657;G:1005901638;T:1241749988;N:0         1263523570 919488657 1005901638 1241749988 0 SRX5108026 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50514 50514 SRR8293428 SRX5108025 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 1 2K E05 1 1 2K E05 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160831_110725_42199_c101016682550000001823228410211624_s1_p0.1.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.2.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.3.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 4610281055.0 163482.0 1 2K E05 1.tar.gz   A:1313884618;C:971754386;G:994748647;T:1264950574;N:0         1313884618 971754386 994748647 1264950574 0 SRX5108025 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50515 50515 SRR8293429 SRX5108024 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 1 2K F03 1 1 2K F03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160828_213458_42199_c101055502550000001823247601061765_s1_p0.1.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.2.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.3.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 6488051235.0 163482.0 1 2K F03 1.tar.gz   A:1835535517;C:1366315971;G:1464006715;T:1752025312;N:0         1835535517 1366315971 1464006715 1752025312 0 SRX5108024 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50516 50516 SRR8293430 SRX5108023 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 1 2K G05 1 1 2K G05 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160829_020016_42199_c101055502550000001823247601061766_s1_p0.1.bax.h5 m160829_020016_42199_c101055502550000001823247601061766_s1_p0.2.bax.h5 m160829_020016_42199_c101055502550000001823247601061766_s1_p0.3.bax.h5 m160829_020016_42199_c101055502550000001823247601061766_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 6326394753.0 163482.0 1 2K G03 1.tar.gz   A:1844148357;C:1410947877;G:1367704673;T:1640545786;N:0         1844148357 1410947877 1367704673 1640545786 0 SRX5108023 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50517 50517 SRR8293431 SRX5108022 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 0.5 1K E03 1 0.5 1K E03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160921_142928_42199_c100979762550000001823212907061657_s1_X0.bas.h5 m160921_142928_42199_c100979762550000001823212907061657_s1_X0.3.bax.h5 m160921_142928_42199_c100979762550000001823212907061657_s1_X0.2.bax.h5 m160921_142928_42199_c100979762550000001823212907061657_s1_X0.1.bax.h5 pacbio_native pacbio_native pacbio_native pacbio_native 1906153604.0 163482.0 0.5 1K E03 1.tar.gz   A:568523342;C:407560314;G:389613623;T:500427928;N:0         568523342 407560314 389613623 500427928 0 SRX5108022 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-08 Zygote Embryo Gonad Reproductive System
50518 50518 SRR8293432 SRX5108021 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 0.5 1K F03 1 0.5 1K F03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160921_184841_42199_c101055652550000001823238312291647_s1_p0.1.bax.h5 m160921_184841_42199_c101055652550000001823238312291647_s1_p0.2.bax.h5 m160921_184841_42199_c101055652550000001823238312291647_s1_p0.3.bax.h5 m160921_184841_42199_c101055652550000001823238312291647_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 4156910909.0 163482.0 0.5 1K F03 1.tar.gz   A:1183616929;C:878682771;G:890537851;T:1136413189;N:0         1183616929 878682771 890537851 1136413189 0 SRX5108021 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50519 50519 SRR8293433 SRX5108020 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 0.5 1K G03 1 0.5 1K G03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160829_063211_42199_c101055502550000001823247601061767_s1_p0.bas.h5 m160829_063211_42199_c101055502550000001823247601061767_s1_p0.3.bax.h5 m160829_063211_42199_c101055502550000001823247601061767_s1_p0.2.bax.h5 m160829_063211_42199_c101055502550000001823247601061767_s1_p0.1.bax.h5 pacbio_native pacbio_native pacbio_native pacbio_native 5660530911.0 163482.0 0.5 1K H03 1.tar.gz   A:1835439052;C:1406954743;G:1037776936;T:1307349158;N:0         1835439052 1406954743 1037776936 1307349158 0 SRX5108020 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-08 Zygote Embryo Gonad Reproductive System
50520 50520 SRR8293434 SRX5108019 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 1 2K C05 1 1 2K C05 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160831_022615_42199_c101016682550000001823228410211622_s1_p0.1.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.2.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.3.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 3047073997.0 163482.0 1 2K C05 1.tar.gz   A:862003813;C:632482019;G:660595007;T:843966997;N:0         862003813 632482019 660595007 843966997 0 SRX5108019 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50521 50521 SRR8293435 SRX5108018 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 2 3K F05 1 2 3K F05 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160831_152721_42199_c101016682550000001823228410211625_s1_p0.1.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.2.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.3.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 3504827488.0 163482.0 2 3K F05 1.tar.gz   A:1035191904;C:787763869;G:735456262;T:916015813;N:0         1035191904 787763869 735456262 916015813 0 SRX5108018 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50522 50522 SRR8293436 SRX5108017 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 2 3K G05 1 2 3K G05 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160831_195005_42199_c101016682550000001823228410211626_s1_p0.1.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.2.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.3.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 3325636539.0 163482.0 2 3K G05 1.tar.gz   A:945624516;C:712334057;G:726829680;T:910995593;N:0         945624516 712334057 726829680 910995593 0 SRX5108017 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50523 50523 SRR8293437 SRX5108016 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 2 3K H05 1 2 3K H05 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160901_000924_42199_c101016682550000001823228410211627_s1_p0.1.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.2.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.3.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 3219471171.0 163482.0 2 3K H05 1.tar.gz   A:955121582;C:717645710;G:671893655;T:846270302;N:0         955121582 717645710 671893655 846270302 0 SRX5108016 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50524 50524 SRR8293438 SRX5108015 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 3K plus C03 1 3K plus C03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160828_083719_42199_c101055502550000001823247601061762_s1_p0.1.bax.h5 m160828_083719_42199_c101055502550000001823247601061762_s1_p0.2.bax.h5 m160828_083719_42199_c101055502550000001823247601061762_s1_p0.3.bax.h5 m160828_083719_42199_c101055502550000001823247601061762_s1_p0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 2241335194.0 163482.0 3K plus C03 1.tar.gz   A:714450646;C:554512788;G:442348736;T:513624736;N:0         714450646 554512788 442348736 513624736 0 SRX5108015 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50525 50525 SRR8293439 SRX5108014 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 3K plus G03 1 3K plus G03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160921_231100_42199_c100829932550000001823181912311557_s1_X0.1.bax.h5 m160921_231100_42199_c100829932550000001823181912311557_s1_X0.2.bax.h5 m160921_231100_42199_c100829932550000001823181912311557_s1_X0.3.bax.h5 m160921_231100_42199_c100829932550000001823181912311557_s1_X0.bas.h5 pacbio_native pacbio_native pacbio_native pacbio_native 1500621674.0 163482.0 3K plus G03 1.tar.gz   A:461686513;C:353289117;G:298838025;T:376337789;N:0         461686513 353289117 298838025 376337789 0 SRX5108014 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-10 Zygote Embryo Gonad Reproductive System
50526 50526 SRR8293440 SRX5108013 SRS4117494 SRP172911 PRJNA498365 Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio PRJNA498365 Other Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.     Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M. Model organism or animal sample from Danio rerio unfertilized eggs from three female zebrafish were harvested as samples Z1 M   isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs. 3K plus H03 1 3K plus H03 1 RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.     RNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE PACBIO_SMRT PacBio RS II   SRP172911     m160922_033036_42199_c101055332550000001823238312291650_s1_p0.bas.h5 m160922_033036_42199_c101055332550000001823238312291650_s1_p0.3.bax.h5 m160922_033036_42199_c101055332550000001823238312291650_s1_p0.2.bax.h5 m160922_033036_42199_c101055332550000001823238312291650_s1_p0.1.bax.h5 pacbio_native pacbio_native pacbio_native pacbio_native 2783940881.0 163482.0 3K plus H03 1.tar.gz   A:785688594;C:616582821;G:618500268;T:743803835;N:0         785688594 616582821 618500268 743803835 0 SRX5108013 SRS4117494 SRA820074 Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio Institute of Hydrobiology, Chinese Academy of Sciences                             pacbio pacbio_early full_length random_priming unknown bulk unknown unknown   China 2018-12-08 Zygote Embryo Gonad Reproductive System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;