run_metadata
10 rows where experiment.platform = "DNBSEQ" and tissue_curation = "Spinal Cord"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 44 | 44 | DRR668250 | DRX648352 | DRS458865 | DRP012880 | PRJDB18466 | Comparison of spinal cord regeneration capacity in zebrafish and medaka | PRJDB18466 | Other | Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability. | pubmed:40278963 | Zebrafish 2 weeks post spinal cord injury replicate 3 | Zebrafish 2wpi 3 | SAMD00799623 | sample name:Zebrafish 2wpi 3|biological replicate:3|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 25|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord | DNBSEQ G400 paired end sequencing of SAMD00799623 | DRX648352 | RNA seq of spinal cord in zebrafish at 2wpi injured 3 | 1 | Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | DRP012880 | DNBSEQ G400 paired end sequencing of SAMD00799623 | 14782516800.0 | 73912584.0 | DRR668250 | 0:100 1:100 | A:4058090278;C:3335994894;G:3323563782;T:4062467903;N:2399943 | 100 | 100 | 4058090278 | 3335994894 | 3323563782 | 4062467903 | 2399943 | DRX648352 | DRS458865 | DRA020617 | Osaka University | Osaka University | B | B | biological fallback assumption | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | Japan | 2025-05-12 | Adult | Adult | Spinal Cord | Nervous System | |||||||||||||||||||||||||||||
| 45 | 45 | DRR668249 | DRX648351 | DRS458864 | DRP012880 | PRJDB18466 | Comparison of spinal cord regeneration capacity in zebrafish and medaka | PRJDB18466 | Other | Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability. | pubmed:40278963 | Zebrafish 2 weeks post spinal cord injury replicate 2 | Zebrafish 2wpi 2 | SAMD00799622 | sample name:Zebrafish 2wpi 2|biological replicate:2|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 14|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord | DNBSEQ G400 paired end sequencing of SAMD00799622 | DRX648351 | RNA seq of spinal cord in zebrafish at 2wpi injured 2 | 1 | Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | DRP012880 | DNBSEQ G400 paired end sequencing of SAMD00799622 | 13687641800.0 | 68438209.0 | DRR668249 | 0:100 1:100 | A:3759784620;C:3087398782;G:3083881581;T:3754378915;N:2197902 | 100 | 100 | 3759784620 | 3087398782 | 3083881581 | 3754378915 | 2197902 | DRX648351 | DRS458864 | DRA020617 | Osaka University | Osaka University | B | B | biological fallback assumption | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | Japan | 2025-05-12 | Adult | Adult | Spinal Cord | Nervous System | |||||||||||||||||||||||||||||
| 46 | 46 | DRR668248 | DRX648350 | DRS458863 | DRP012880 | PRJDB18466 | Comparison of spinal cord regeneration capacity in zebrafish and medaka | PRJDB18466 | Other | Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability. | pubmed:40278963 | Zebrafish 2 weeks post spinal cord injury replicate 1 | Zebrafish 2wpi 1 | SAMD00799621 | sample name:Zebrafish 2wpi 1|biological replicate:1|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 14|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord | DNBSEQ G400 paired end sequencing of SAMD00799621 | DRX648350 | RNA seq of spinal cord in zebrafish at 2wpi injured 1 | 1 | Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | DRP012880 | DNBSEQ G400 paired end sequencing of SAMD00799621 | 16376197200.0 | 81880986.0 | DRR668248 | 0:100 1:100 | A:4485868844;C:3700974430;G:3710833937;T:4475827800;N:2692189 | 100 | 100 | 4485868844 | 3700974430 | 3710833937 | 4475827800 | 2692189 | DRX648350 | DRS458863 | DRA020617 | Osaka University | Osaka University | B | B | biological fallback assumption | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | Japan | 2025-05-12 | Adult | Adult | Spinal Cord | Nervous System | |||||||||||||||||||||||||||||
| 47 | 47 | DRR668247 | DRX648349 | DRS458862 | DRP012880 | PRJDB18466 | Comparison of spinal cord regeneration capacity in zebrafish and medaka | PRJDB18466 | Other | Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability. | pubmed:40278963 | Zebrafish Intact biological replicate 3 | Zebrafish Control 3 | SAMD00799620 | sample name:Zebrafish Control 3|biological replicate:3|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 21|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord | DNBSEQ G400 paired end sequencing of SAMD00799620 | DRX648349 | RNA seq of spinal cord in zebrafish at 0wpi control 3 | 1 | Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | DRP012880 | DNBSEQ G400 paired end sequencing of SAMD00799620 | 13377538600.0 | 66887693.0 | DRR668247 | 0:100 1:100 | A:3725064764;C:2973653932;G:2980883214;T:3695767890;N:2168800 | 100 | 100 | 3725064764 | 2973653932 | 2980883214 | 3695767890 | 2168800 | DRX648349 | DRS458862 | DRA020617 | Osaka University | Osaka University | B | B | biological fallback assumption | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | Japan | 2025-05-12 | Adult | Adult | Spinal Cord | Nervous System | |||||||||||||||||||||||||||||
| 48 | 48 | DRR668246 | DRX648348 | DRS458861 | DRP012880 | PRJDB18466 | Comparison of spinal cord regeneration capacity in zebrafish and medaka | PRJDB18466 | Other | Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability. | pubmed:40278963 | Zebrafish Intact biological replicate 2 | Zebrafish Control 2 | SAMD00799619 | sample name:Zebrafish Control 2|biological replicate:2|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2023 04 21|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord | DNBSEQ G400 paired end sequencing of SAMD00799619 | DRX648348 | RNA seq of spinal cord in zebrafish at 0wpi control 2 | 1 | Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | DRP012880 | DNBSEQ G400 paired end sequencing of SAMD00799619 | 14971411400.0 | 74857057.0 | DRR668246 | 0:100 1:100 | A:4160326445;C:3329083037;G:3329123314;T:4150453700;N:2424904 | 100 | 100 | 4160326445 | 3329083037 | 3329123314 | 4150453700 | 2424904 | DRX648348 | DRS458861 | DRA020617 | Osaka University | Osaka University | B | B | biological fallback assumption | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | Japan | 2025-05-12 | Adult | Adult | Spinal Cord | Nervous System | |||||||||||||||||||||||||||||
| 49 | 49 | DRR668245 | DRX648347 | DRS458860 | DRP012880 | PRJDB18466 | Comparison of spinal cord regeneration capacity in zebrafish and medaka | PRJDB18466 | Other | Unlike mammals zebrafish have the remarkable ability to regenerate many tissues including the spinal cord. Medaka another model fish species has a low regenerative ability in the spinal cord. Therefore comparisons with them advantageous to revealing regeneration specific mechanisms in the spinal cord. The comparison of the spinal cord regeneration abilities of zebrafish and medaka could be a promising research field to elucidate new factors that determine spinal cord regeneration ability. | pubmed:40278963 | Zebrafish Intact biological replicate 1 | Zebrafish Control 1 | SAMD00799618 | sample name:Zebrafish Control 1|biological replicate:1|biomaterial provider:Center of Medical Innovation and Translational Research Osaka University|collection date:2024 05 04|dev stage:Adult|geo loc name:Japan|sex:not determined|strain:AB Zebrafish|tissue:Spinal cord | DNBSEQ G400 paired end sequencing of SAMD00799618 | DRX648347 | RNA seq of spinal cord in zebrafish at 0wpi control 1 | 1 | Total RNA was extracted using RNeasy Micro Kit Qiagen 74104 with DNase treatment RNase Free DNase Set Qiagen 79254. Libraries were constructed from the amplified total RNA. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | DRP012880 | DNBSEQ G400 paired end sequencing of SAMD00799618 | 13912523800.0 | 69562619.0 | DRR668245 | 0:100 1:100 | A:3888902049;C:3079617959;G:3075111814;T:3866655202;N:2236776 | 100 | 100 | 3888902049 | 3079617959 | 3075111814 | 3866655202 | 2236776 | DRX648347 | DRS458860 | DRA020617 | Osaka University | Osaka University | B | B | biological fallback assumption | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | Japan | 2025-05-12 | Adult | Adult | Spinal Cord | Nervous System | |||||||||||||||||||||||||||||
| 71409 | 71409 | SRR21590947 | SRX17592668 | SRS15130333 | SRP397443 | PRJNA880627 | Single cell sequencing of WT and hb egfaKO zebrafish spinal cord at 1 week post sham or transection injury. | GSE213435 | Transcriptome Analysis | We identified hb egfa as a secreted factor necessary and sufficient for spinal cord regeneration in zebrafish. To elucidate the Hb egfa mechanism of action we perforemd single cell sequencing of WT and hb egfaKO zebrafish spinal cord. Overall design: Adult zebrafish were subjected to spinal cord transection. 2 mm rostral and caudal to the transection site were collected at 1 xxx post injury. Spinal cord of sham injured animals was collected as control. Samples were and subjected to the 10X Genomics pipeline for single cell sequencing 4 samples in total WT 1 wpi WT sham hb egfaKO 1 wpi hb egfaKO sham. | pubmed:37567873 | danRer11 WT 7dpi | GSM6586115 | source name:spinal cord|genotype:WT|tissue:spinal cord|strain:Ekkwill EK|age:6 month|treatment:7dpi|geo loc name:missing|collection date:missing | danRer11 WT 7dpi | The demultiplexing barcoded processing gene counting and aggregation were made using the Cell Ranger software v6.0.1 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/what is cell ranger Assembly: danRer11 Supplementary files format and content: h5 files for count table | spinal cord | Samples were processed following the 10X Chromium platform following the manufacturer’s guidelines using 10X Single Cell 3’ v3 chemistry 10X Genomics Pleasanton USA. | genotype:WT|tissue:spinal cord|strain:Ekkwill EK|age:6 month|treatment:7dpi | GSM6586115 | GSM6586115: danRer11 WT 7dpi; Danio rerio; RNA Seq | GSM6586115 r1 | GSM6586115 | 1 | Samples were processed following the 10X Chromium platform following the manufacturer's guidelines using 10X Single Cell three prime v3 chemistry 10X Genomics Pleasanton USA. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | SRP397443 | KP_VC_sample4_S1_L001_R2_001.fastq.gz KP_VC_sample4_S1_L001_R1_001.fastq.gz | fastq fastq | 50454681480.0 | 423988920.0 | GSM6586115 r1 | 0:28 1:91 | A:14159328348;C:10820572193;G:11486998159;T:13985883249;N:1899531 | 28 | 91 | 14159328348 | 10820572193 | 11486998159 | 13985883249 | 1899531 | SRX17592668 | SRS15130333 | SRA1500311 | Cell biology, Duke University | Poss, Cell Biology, Duke University | 2 | 0.00753 | 0.88935 | 0.00224 | 0.18283 | 0.98528 | 0.77656 | 0.24704 | 0.5609 | 28 | 91 | T | B | sc-like readlen | bgi | bgi | unknown | cdna_unspecified | unknown | sc | single_cell_droplet | 10x | United States | 2022-09-15 | Adult | Adult | Spinal Cord | Nervous System | ||||||||||||
| 71410 | 71410 | SRR21590948 | SRX17592667 | SRS15130332 | SRP397443 | PRJNA880627 | Single cell sequencing of WT and hb egfaKO zebrafish spinal cord at 1 week post sham or transection injury. | GSE213435 | Transcriptome Analysis | We identified hb egfa as a secreted factor necessary and sufficient for spinal cord regeneration in zebrafish. To elucidate the Hb egfa mechanism of action we perforemd single cell sequencing of WT and hb egfaKO zebrafish spinal cord. Overall design: Adult zebrafish were subjected to spinal cord transection. 2 mm rostral and caudal to the transection site were collected at 1 xxx post injury. Spinal cord of sham injured animals was collected as control. Samples were and subjected to the 10X Genomics pipeline for single cell sequencing 4 samples in total WT 1 wpi WT sham hb egfaKO 1 wpi hb egfaKO sham. | pubmed:37567873 | danRer11 WT sham | GSM6586114 | source name:spinal cord|genotype:WT|tissue:spinal cord|strain:Ekkwill EK|age:6 month|treatment:sham|geo loc name:missing|collection date:missing | danRer11 WT sham | The demultiplexing barcoded processing gene counting and aggregation were made using the Cell Ranger software v6.0.1 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/what is cell ranger Assembly: danRer11 Supplementary files format and content: h5 files for count table | spinal cord | Samples were processed following the 10X Chromium platform following the manufacturer’s guidelines using 10X Single Cell 3’ v3 chemistry 10X Genomics Pleasanton USA. | genotype:WT|tissue:spinal cord|strain:Ekkwill EK|age:6 month|treatment:sham | GSM6586114 | GSM6586114: danRer11 WT sham; Danio rerio; RNA Seq | GSM6586114 r1 | GSM6586114 | 1 | Samples were processed following the 10X Chromium platform following the manufacturer's guidelines using 10X Single Cell three prime v3 chemistry 10X Genomics Pleasanton USA. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | SRP397443 | KP_VC_sample3_S1_L001_R1_001.fastq.gz KP_VC_sample3_S1_L001_R2_001.fastq.gz | fastq fastq | 49434606578.0 | 415416862.0 | GSM6586114 r1 | 0:28 1:91 | A:13689985840;C:10750469002;G:11165716773;T:13825714788;N:2720175 | 28 | 91 | 13689985840 | 10750469002 | 11165716773 | 13825714788 | 2720175 | SRX17592667 | SRS15130332 | SRA1500311 | Cell biology, Duke University | Poss, Cell Biology, Duke University | 2 | 0.01019 | 0.90181 | 0.00309 | 0.16838 | 0.98522 | 0.78459 | 0.27523 | 0.51926 | 28 | 91 | T | B | sc-like readlen | bgi | bgi | unknown | cdna_unspecified | unknown | sc | single_cell_droplet | 10x | United States | 2022-09-15 | Adult | Adult | Spinal Cord | Nervous System | ||||||||||||
| 71411 | 71411 | SRR21590949 | SRX17592666 | SRS15130331 | SRP397443 | PRJNA880627 | Single cell sequencing of WT and hb egfaKO zebrafish spinal cord at 1 week post sham or transection injury. | GSE213435 | Transcriptome Analysis | We identified hb egfa as a secreted factor necessary and sufficient for spinal cord regeneration in zebrafish. To elucidate the Hb egfa mechanism of action we perforemd single cell sequencing of WT and hb egfaKO zebrafish spinal cord. Overall design: Adult zebrafish were subjected to spinal cord transection. 2 mm rostral and caudal to the transection site were collected at 1 xxx post injury. Spinal cord of sham injured animals was collected as control. Samples were and subjected to the 10X Genomics pipeline for single cell sequencing 4 samples in total WT 1 wpi WT sham hb egfaKO 1 wpi hb egfaKO sham. | pubmed:37567873 | danRer11 hbegfaKO 7dpi | GSM6586113 | source name:spinal cord|genotype:hbegfaKO|tissue:spinal cord|strain:Ekkwill EK|age:6 month|treatment:7dpi|geo loc name:missing|collection date:missing | danRer11 hbegfaKO 7dpi | The demultiplexing barcoded processing gene counting and aggregation were made using the Cell Ranger software v6.0.1 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/what is cell ranger Assembly: danRer11 Supplementary files format and content: h5 files for count table | spinal cord | Samples were processed following the 10X Chromium platform following the manufacturer’s guidelines using 10X Single Cell 3’ v3 chemistry 10X Genomics Pleasanton USA. | genotype:hbegfaKO|tissue:spinal cord|strain:Ekkwill EK|age:6 month|treatment:7dpi | GSM6586113 | GSM6586113: danRer11 hbegfaKO 7dpi; Danio rerio; RNA Seq | GSM6586113 r1 | GSM6586113 | 1 | Samples were processed following the 10X Chromium platform following the manufacturer's guidelines using 10X Single Cell three prime v3 chemistry 10X Genomics Pleasanton USA. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | SRP397443 | KP_VC_sample2_S1_L001_R1_001.fastq.gz KP_VC_sample2_S1_L001_R2_001.fastq.gz | fastq fastq | 48346471411.0 | 406272869.0 | GSM6586113 r1 | 0:28 1:91 | A:13278513523;C:10826562396;G:11318717473;T:12918171753;N:4506266 | 28 | 91 | 13278513523 | 10826562396 | 11318717473 | 12918171753 | 4506266 | SRX17592666 | SRS15130331 | SRA1500311 | Cell biology, Duke University | Poss, Cell Biology, Duke University | 2 | 0.01379 | 0.89003 | 0.00446 | 0.1909 | 0.97808 | 0.78415 | 0.32888 | 0.6007 | 28 | 91 | T | B | sc-like readlen | bgi | bgi | unknown | cdna_unspecified | unknown | sc | single_cell_droplet | 10x | United States | 2022-09-15 | Adult | Adult | Spinal Cord | Nervous System | ||||||||||||
| 71412 | 71412 | SRR21590950 | SRX17592665 | SRS15130330 | SRP397443 | PRJNA880627 | Single cell sequencing of WT and hb egfaKO zebrafish spinal cord at 1 week post sham or transection injury. | GSE213435 | Transcriptome Analysis | We identified hb egfa as a secreted factor necessary and sufficient for spinal cord regeneration in zebrafish. To elucidate the Hb egfa mechanism of action we perforemd single cell sequencing of WT and hb egfaKO zebrafish spinal cord. Overall design: Adult zebrafish were subjected to spinal cord transection. 2 mm rostral and caudal to the transection site were collected at 1 xxx post injury. Spinal cord of sham injured animals was collected as control. Samples were and subjected to the 10X Genomics pipeline for single cell sequencing 4 samples in total WT 1 wpi WT sham hb egfaKO 1 wpi hb egfaKO sham. | pubmed:37567873 | danRer11 hbegfaKO sham | GSM6586112 | source name:spinal cord|genotype:hbegfaKO|tissue:spinal cord|strain:Ekkwill EK|age:6 month|treatment:sham|geo loc name:missing|collection date:missing | danRer11 hbegfaKO sham | The demultiplexing barcoded processing gene counting and aggregation were made using the Cell Ranger software v6.0.1 https://support.10xgenomics.com/single cell gene expression/software/pipelines/latest/what is cell ranger Assembly: danRer11 Supplementary files format and content: h5 files for count table | spinal cord | Samples were processed following the 10X Chromium platform following the manufacturer’s guidelines using 10X Single Cell 3’ v3 chemistry 10X Genomics Pleasanton USA. | genotype:hbegfaKO|tissue:spinal cord|strain:Ekkwill EK|age:6 month|treatment:sham | GSM6586112 | GSM6586112: danRer11 hbegfaKO sham; Danio rerio; RNA Seq | GSM6586112 r1 | GSM6586112 | 1 | Samples were processed following the 10X Chromium platform following the manufacturer's guidelines using 10X Single Cell three prime v3 chemistry 10X Genomics Pleasanton USA. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | cDNA | PAIRED | DNBSEQ | DNBSEQ-G400 | SRP397443 | KP_VC_sample1_S1_L001_R1_001.fastq.gz KP_VC_sample1_S1_L001_R2_001.fastq.gz | fastq fastq | 50009919813.0 | 420251427.0 | GSM6586112 r1 | 0:28 1:91 | A:13721824199;C:11296818044;G:11807686254;T:13179637678;N:3953638 | 28 | 91 | 13721824199 | 11296818044 | 11807686254 | 13179637678 | 3953638 | SRX17592665 | SRS15130330 | SRA1500311 | Cell biology, Duke University | Poss, Cell Biology, Duke University | 2 | 0.01254 | 0.83945 | 0.00371 | 0.16358 | 0.98378 | 0.80728 | 0.25895 | 0.57907 | 28 | 91 | T | B | sc-like readlen | bgi | bgi | unknown | cdna_unspecified | unknown | sc | single_cell_droplet | 10x | United States | 2022-09-15 | Adult | Adult | Spinal Cord | Nervous System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;