run_metadata
14 rows where experiment.library_source = "TRANSCRIPTOMIC", technology = "bulk" and tissue_curation_coarse = "Embryo Imprecise"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 6 | 6 | DRR315802 | DRX305194 | DRS231989 | DRP008318 | PRJDB12206 | Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes | DRP008318 | Transcriptome Analysis | Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant "rw147" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis. | wildtype sibling sample4 | SAMD00400823 | sample name:rw147 2.5dpf wildtype rep 4|biological replicate:4 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400823 | DRX305194 | 1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>151</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>76</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP008318 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400823 | 10262353995.0 | 34151547.0 | DRR315802 | 0:150.27 1:150.22 | A:2735078560;C:2386126821;G:2433638250;T:2707202814;N:307550 | 150 | 150 | 2735078560 | 2386126821 | 2433638250 | 2707202814 | 307550 | DRX305194 | DRS231989 | DRA012640 | OIST|Developmental Neurobiology Unit | Developmental Neurobiology Unit | 2 | 0.95231 | 0.95295 | 0.09229 | 0.08773 | 0.71819 | 0.72107 | 0.46746 | 0.46617 | 151 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Unknown | 2022-03-16 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 7 | 7 | DRR315801 | DRX305193 | DRS231988 | DRP008318 | PRJDB12206 | Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes | DRP008318 | Transcriptome Analysis | Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant "rw147" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis. | wildtype sibling sample3 | SAMD00400822 | sample name:rw147 2.5dpf wildtype rep 3|biological replicate:3 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400822 | DRX305193 | 1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>151</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>76</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP008318 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400822 | 11516368634.0 | 38355888.0 | DRR315801 | 0:150.15 1:150.10 | A:3080341643;C:2678048339;G:2713051368;T:3044449330;N:477954 | 150 | 150 | 3080341643 | 2678048339 | 2713051368 | 3044449330 | 477954 | DRX305193 | DRS231988 | DRA012640 | OIST|Developmental Neurobiology Unit | Developmental Neurobiology Unit | 2 | 0.95353 | 0.95634 | 0.08909 | 0.08533 | 0.71374 | 0.71252 | 0.45986 | 0.46059 | 150 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Unknown | 2022-03-16 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 8 | 8 | DRR315800 | DRX305192 | DRS231987 | DRP008318 | PRJDB12206 | Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes | DRP008318 | Transcriptome Analysis | Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant "rw147" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis. | wildtype sibling sample2 | SAMD00400821 | sample name:rw147 2.5dpf wildtype rep 2|biological replicate:2 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400821 | DRX305192 | 1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>151</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>76</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP008318 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400821 | 8814057148.0 | 29367513.0 | DRR315800 | 0:150.09 1:150.04 | A:2350403211;C:2054073465;G:2083044327;T:2326181188;N:354957 | 150 | 150 | 2350403211 | 2054073465 | 2083044327 | 2326181188 | 354957 | DRX305192 | DRS231987 | DRA012640 | OIST|Developmental Neurobiology Unit | Developmental Neurobiology Unit | 2 | 0.95287 | 0.95643 | 0.0891 | 0.08586 | 0.70309 | 0.70252 | 0.46384 | 0.46281 | 151 | 149 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Unknown | 2022-03-16 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 9 | 9 | DRR315799 | DRX305191 | DRS231986 | DRP008318 | PRJDB12206 | Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes | DRP008318 | Transcriptome Analysis | Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant "rw147" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis. | wildtype sibling sample1 | SAMD00400820 | sample name:rw147 2.5dpf wildtype rep 1|biological replicate:1 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400820 | DRX305191 | 1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>151</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>76</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP008318 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400820 | 10491955578.0 | 34900682.0 | DRR315799 | 0:150.34 1:150.28 | A:2796521111;C:2446218287;G:2483414564;T:2765477785;N:323831 | 150 | 150 | 2796521111 | 2446218287 | 2483414564 | 2765477785 | 323831 | DRX305191 | DRS231986 | DRA012640 | OIST|Developmental Neurobiology Unit | Developmental Neurobiology Unit | 2 | 0.9539 | 0.95646 | 0.08185 | 0.07808 | 0.70025 | 0.70013 | 0.44713 | 0.44987 | 150 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Unknown | 2022-03-16 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 10 | 10 | DRR315798 | DRX305190 | DRS231985 | DRP008318 | PRJDB12206 | Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes | DRP008318 | Transcriptome Analysis | Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant "rw147" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis. | strip1 mutant sample4 | SAMD00400819 | sample name:rw147 2.5dpf Mutant rep 4|biological replicate:4 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400819 | DRX305190 | 1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>151</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>76</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP008318 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400819 | 9197802250.0 | 30604326.0 | DRR315798 | 0:150.30 1:150.24 | A:2468967963;C:2130949980;G:2158692262;T:2438931017;N:261028 | 150 | 150 | 2468967963 | 2130949980 | 2158692262 | 2438931017 | 261028 | DRX305190 | DRS231985 | DRA012640 | OIST|Developmental Neurobiology Unit | Developmental Neurobiology Unit | 2 | 0.95159 | 0.95439 | 0.10146 | 0.09758 | 0.71995 | 0.71983 | 0.46519 | 0.46797 | 150 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Unknown | 2022-03-16 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 11 | 11 | DRR315797 | DRX305189 | DRS231984 | DRP008318 | PRJDB12206 | Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes | DRP008318 | Transcriptome Analysis | Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant "rw147" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis. | strip1 mutant sample3 | SAMD00400818 | sample name:rw147 2.5dpf Mutant rep 3|biological replicate:3 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400818 | DRX305189 | 1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>151</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>76</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP008318 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400818 | 10498982078.0 | 34931731.0 | DRR315797 | 0:150.31 1:150.25 | A:2804535103;C:2445295179;G:2478768789;T:2770066062;N:316945 | 150 | 150 | 2804535103 | 2445295179 | 2478768789 | 2770066062 | 316945 | DRX305189 | DRS231984 | DRA012640 | OIST|Developmental Neurobiology Unit | Developmental Neurobiology Unit | 2 | 0.95448 | 0.95652 | 0.0939 | 0.0887 | 0.71796 | 0.71847 | 0.46335 | 0.46615 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Unknown | 2022-03-16 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 12 | 12 | DRR315796 | DRX305188 | DRS231983 | DRP008318 | PRJDB12206 | Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes | DRP008318 | Transcriptome Analysis | Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant "rw147" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis. | strip1 mutant sample2 | SAMD00400817 | sample name:rw147 2.5dpf Mutant rep 2|biological replicate:2 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400817 | DRX305188 | 1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>151</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>76</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP008318 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400817 | 9850145990.0 | 32782079.0 | DRR315796 | 0:150.26 1:150.21 | A:2636205537;C:2286508705;G:2319481100;T:2607600624;N:350024 | 150 | 150 | 2636205537 | 2286508705 | 2319481100 | 2607600624 | 350024 | DRX305188 | DRS231983 | DRA012640 | OIST|Developmental Neurobiology Unit | Developmental Neurobiology Unit | 2 | 0.95193 | 0.95472 | 0.09722 | 0.09375 | 0.7138 | 0.71299 | 0.45542 | 0.45994 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Unknown | 2022-03-16 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 13 | 13 | DRR315795 | DRX305187 | DRS231982 | DRP008318 | PRJDB12206 | Transcriptome analysis of strip1 mutant and wildtype zebrafish eyes | DRP008318 | Transcriptome Analysis | Strip1 plays essential roles in the developing zebrafish retinal neural circuit. To identify the underlying molecular mechanisms at the transcriptomic level transcriptome of strip1 mutant "rw147" eye cups at 2.5 dpf was compared to that of wild type siblings using bulk RNA sequencing analysis. | strip1 mutant sample1 | SAMD00400816 | sample name:rw147 2.5dpf Mutant rep 1|biological replicate:1 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400816 | DRX305187 | 1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>151</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>76</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP008318 | Illumina NovaSeq 6000 paired end sequencing of SAMD00400816 | 9542039835.0 | 31780260.0 | DRR315795 | 0:150.15 1:150.10 | A:2543384204;C:2224374632;G:2258183435;T:2515655339;N:442225 | 150 | 150 | 2543384204 | 2224374632 | 2258183435 | 2515655339 | 442225 | DRX305187 | DRS231982 | DRA012640 | OIST|Developmental Neurobiology Unit | Developmental Neurobiology Unit | 2 | 0.9528 | 0.95591 | 0.08656 | 0.0828 | 0.70352 | 0.70331 | 0.45316 | 0.44914 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Unknown | 2022-03-16 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 59089 | 59089 | SRR11590436 | SRX8157791 | SRS6519805 | SRP257942 | PRJNA627467 | Fli1+ cells transcriptional analysis reveals LMO2 PRDM16 axis in angiogenesis | GSE149152 | Transcriptome Analysis | Different GFP+ cell population were isolated from Tgfli1:EGFP zebrafish embryos. Novel roles of epigenetic modifiers in endothelial differentiation were identified. Overall design: Each sample has been run in duplicate with 2 files per each run | pubmed:34330825 | Low Fli1+ rep2 | GSM4491074 | tissue:Low Fli1+|cell population:Low Fli1+|genotype:Tgfli1:EGFP | Low Fli1+ rep2 | Illumina Illumina Hi Seq 2500 software used for basecalling. Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to dr10 whole genome Fragments Per Kilobase of exon per Megabase of library size FPKM were calculated. Genome build: danio rerio10 Supplementary files format and content: Text files with FPKM values for each sample were grouped in a single excel file | Low Fli1+ | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | cell population:Low Fli1+|genotype:Tgfli1:EGFP | GSM4491074 | GSM4491074: Low Fli1+ rep2; Danio rerio; RNA Seq | GSM4491074 | 1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | GEO Accession:GSM4491074 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP257942 | CI5758_GGCTAC_L005_R1_001.fastq.gz CI5758_GGCTAC_L005_R2_001.fastq.gz | fastq fastq | 9251096400.0 | 46255482.0 | GSM4491074 r1 | 0:100 1:100 | A:1769289964;C:2789674423;G:2860116416;T:1825482073;N:6533524 | 100 | 100 | 1769289964 | 2789674423 | 2860116416 | 1825482073 | 6533524 | SRX8157791 | SRS6519805 | SRA1068019 | GEO | University of Edinburgh | 2 | 0.90817 | 0.91357 | 0.12465 | 0.1278 | 0.77717 | 0.78376 | 0.75104 | 0.7256 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | United Kingdom | 2020-04-22 | Undetermined | Embryo | Undetermined | Embryo Imprecise | ||||||||||||
| 59090 | 59090 | SRR11590435 | SRX8157790 | SRS6519799 | SRP257942 | PRJNA627467 | Fli1+ cells transcriptional analysis reveals LMO2 PRDM16 axis in angiogenesis | GSE149152 | Transcriptome Analysis | Different GFP+ cell population were isolated from Tgfli1:EGFP zebrafish embryos. Novel roles of epigenetic modifiers in endothelial differentiation were identified. Overall design: Each sample has been run in duplicate with 2 files per each run | pubmed:34330825 | Low Fli1+ rep1 | GSM4491073 | tissue:Low Fli1+|cell population:Low Fli1+|genotype:Tgfli1:EGFP | Low Fli1+ rep1 | Illumina Illumina Hi Seq 2500 software used for basecalling. Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to dr10 whole genome Fragments Per Kilobase of exon per Megabase of library size FPKM were calculated. Genome build: danio rerio10 Supplementary files format and content: Text files with FPKM values for each sample were grouped in a single excel file | Low Fli1+ | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | cell population:Low Fli1+|genotype:Tgfli1:EGFP | GSM4491073 | GSM4491073: Low Fli1+ rep1; Danio rerio; RNA Seq | GSM4491073 | 1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | GEO Accession:GSM4491073 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP257942 | CI5703_CAGATC_L005_R1_001.fastq.gz CI5703_CAGATC_L005_R2_001.fastq.gz | fastq fastq | 6814746200.0 | 34073731.0 | GSM4491073 r1 | 0:100 1:100 | A:1332637473;C:2025204245;G:2079298693;T:1372597674;N:5008115 | 100 | 100 | 1332637473 | 2025204245 | 2079298693 | 1372597674 | 5008115 | SRX8157790 | SRS6519799 | SRA1068019 | GEO | University of Edinburgh | 2 | 0.88252 | 0.88837 | 0.1449 | 0.1472 | 0.77839 | 0.78291 | 0.71852 | 0.68399 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | United Kingdom | 2020-04-22 | Undetermined | Embryo | Undetermined | Embryo Imprecise | ||||||||||||
| 59091 | 59091 | SRR11590434 | SRX8157789 | SRS6519800 | SRP257942 | PRJNA627467 | Fli1+ cells transcriptional analysis reveals LMO2 PRDM16 axis in angiogenesis | GSE149152 | Transcriptome Analysis | Different GFP+ cell population were isolated from Tgfli1:EGFP zebrafish embryos. Novel roles of epigenetic modifiers in endothelial differentiation were identified. Overall design: Each sample has been run in duplicate with 2 files per each run | pubmed:34330825 | Fli1 rep2 | GSM4491072 | tissue:Fli1 |cell population:Fli1 |genotype:Tgfli1:EGFP | Fli1 rep2 | Illumina Illumina Hi Seq 2500 software used for basecalling. Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to dr10 whole genome Fragments Per Kilobase of exon per Megabase of library size FPKM were calculated. Genome build: danio rerio10 Supplementary files format and content: Text files with FPKM values for each sample were grouped in a single excel file | Fli1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | cell population:Fli1 |genotype:Tgfli1:EGFP | GSM4491072 | GSM4491072: Fli1 rep2; Danio rerio; RNA Seq | GSM4491072 | 1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | GEO Accession:GSM4491072 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP257942 | CI5757_TAGCTT_L005_R1_001.fastq.gz CI5757_TAGCTT_L005_R2_001.fastq.gz | fastq fastq | 8455897000.0 | 42279485.0 | GSM4491072 r1 | 0:100 1:100 | A:1612223258;C:2555349390;G:2614157473;T:1667931765;N:6235114 | 100 | 100 | 1612223258 | 2555349390 | 2614157473 | 1667931765 | 6235114 | SRX8157789 | SRS6519800 | SRA1068019 | GEO | University of Edinburgh | 2 | 0.94331 | 0.94696 | 0.11995 | 0.12207 | 0.76075 | 0.76796 | 0.74158 | 0.71821 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | United Kingdom | 2020-04-22 | Undetermined | Embryo | Undetermined | Embryo Imprecise | ||||||||||||
| 59092 | 59092 | SRR11590433 | SRX8157788 | SRS6519797 | SRP257942 | PRJNA627467 | Fli1+ cells transcriptional analysis reveals LMO2 PRDM16 axis in angiogenesis | GSE149152 | Transcriptome Analysis | Different GFP+ cell population were isolated from Tgfli1:EGFP zebrafish embryos. Novel roles of epigenetic modifiers in endothelial differentiation were identified. Overall design: Each sample has been run in duplicate with 2 files per each run | pubmed:34330825 | Fli1 rep1 | GSM4491071 | tissue:Fli1 |cell population:Fli1 |genotype:Tgfli1:EGFP | Fli1 rep1 | Illumina Illumina Hi Seq 2500 software used for basecalling. Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to dr10 whole genome Fragments Per Kilobase of exon per Megabase of library size FPKM were calculated. Genome build: danio rerio10 Supplementary files format and content: Text files with FPKM values for each sample were grouped in a single excel file | Fli1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | cell population:Fli1 |genotype:Tgfli1:EGFP | GSM4491071 | GSM4491071: Fli1 rep1; Danio rerio; RNA Seq | GSM4491071 | 1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | GEO Accession:GSM4491071 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP257942 | CI5701_GCCAAT_L005_R1_001.fastq.gz CI5701_GCCAAT_L005_R2_001.fastq.gz | fastq fastq | 7474115600.0 | 37370578.0 | GSM4491071 r1 | 0:100 1:100 | A:1480688513;C:2199814780;G:2256979094;T:1531256742;N:5376471 | 100 | 100 | 1480688513 | 2199814780 | 2256979094 | 1531256742 | 5376471 | SRX8157788 | SRS6519797 | SRA1068019 | GEO | University of Edinburgh | 2 | 0.93558 | 0.94034 | 0.13594 | 0.13917 | 0.75118 | 0.75684 | 0.66654 | 0.68344 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | United Kingdom | 2020-04-22 | Undetermined | Embryo | Undetermined | Embryo Imprecise | ||||||||||||
| 59093 | 59093 | SRR11590438 | SRX8157787 | SRS6519798 | SRP257942 | PRJNA627467 | Fli1+ cells transcriptional analysis reveals LMO2 PRDM16 axis in angiogenesis | GSE149152 | Transcriptome Analysis | Different GFP+ cell population were isolated from Tgfli1:EGFP zebrafish embryos. Novel roles of epigenetic modifiers in endothelial differentiation were identified. Overall design: Each sample has been run in duplicate with 2 files per each run | pubmed:34330825 | High Fli1+ rep2 | GSM4491076 | tissue:High Fli1+|cell population:High Fli1+|genotype:Tgfli1:EGFP | High Fli1+ rep2 | Illumina Illumina Hi Seq 2500 software used for basecalling. Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to dr10 whole genome Fragments Per Kilobase of exon per Megabase of library size FPKM were calculated. Genome build: danio rerio10 Supplementary files format and content: Text files with FPKM values for each sample were grouped in a single excel file | High Fli1+ | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | cell population:High Fli1+|genotype:Tgfli1:EGFP | GSM4491076 | GSM4491076: High Fli1+ rep2; Danio rerio; RNA Seq | GSM4491076 | 1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | GEO Accession:GSM4491076 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP257942 | CI5759_CTTGTA_L005_R1_001.fastq.gz CI5759_CTTGTA_L005_R2_001.fastq.gz | fastq fastq | 6877313200.0 | 34386566.0 | GSM4491076 r1 | 0:100 1:100 | A:1171923636;C:2222996793;G:2262044883;T:1215116923;N:5230965 | 100 | 100 | 1171923636 | 2222996793 | 2262044883 | 1215116923 | 5230965 | SRX8157787 | SRS6519798 | SRA1068019 | GEO | University of Edinburgh | 2 | 0.95139 | 0.95705 | 0.08927 | 0.09017 | 0.81312 | 0.82004 | 0.77768 | 0.75585 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | United Kingdom | 2020-04-22 | Undetermined | Embryo | Undetermined | Embryo Imprecise | ||||||||||||
| 59094 | 59094 | SRR11590437 | SRX8157786 | SRS6519796 | SRP257942 | PRJNA627467 | Fli1+ cells transcriptional analysis reveals LMO2 PRDM16 axis in angiogenesis | GSE149152 | Transcriptome Analysis | Different GFP+ cell population were isolated from Tgfli1:EGFP zebrafish embryos. Novel roles of epigenetic modifiers in endothelial differentiation were identified. Overall design: Each sample has been run in duplicate with 2 files per each run | pubmed:34330825 | High Fli1+ rep1 | GSM4491075 | tissue:High Fli1+|cell population:High Fli1+|genotype:Tgfli1:EGFP | High Fli1+ rep1 | Illumina Illumina Hi Seq 2500 software used for basecalling. Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to dr10 whole genome Fragments Per Kilobase of exon per Megabase of library size FPKM were calculated. Genome build: danio rerio10 Supplementary files format and content: Text files with FPKM values for each sample were grouped in a single excel file | High Fli1+ | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | cell population:High Fli1+|genotype:Tgfli1:EGFP | GSM4491075 | GSM4491075: High Fli1+ rep1; Danio rerio; RNA Seq | GSM4491075 | 1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | GEO Accession:GSM4491075 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP257942 | CI5705_ACTTGA_L005_R1_001.fastq.gz CI5705_ACTTGA_L005_R2_001.fastq.gz | fastq fastq | 8541050000.0 | 42705250.0 | GSM4491075 r1 | 0:100 1:100 | A:1667536155;C:2542273851;G:2600174460;T:1724878449;N:6187085 | 100 | 100 | 1667536155 | 2542273851 | 2600174460 | 1724878449 | 6187085 | SRX8157786 | SRS6519796 | SRA1068019 | GEO | University of Edinburgh | 2 | 0.92757 | 0.93319 | 0.14438 | 0.14609 | 0.78867 | 0.79346 | 0.72736 | 0.71784 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | United Kingdom | 2020-04-22 | Undetermined | Embryo | Undetermined | Embryo Imprecise |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;