run_metadata
22 rows where experiment.library_source = "TRANSCRIPTOMIC", experiment.platform = "BGISEQ" and tissue_curation_coarse = "Reproductive System"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 48812 | 48812 | SRR7341816 | SRX4215318 | SRS3417596 | SRP150521 | PRJNA476105 | Transcriptome analysis of wildtype and sox3 / zebrafish adult ovary | GSE115806 | Transcriptome Analysis | The goals of this study are to compare the differentially expressed genes between wildtype and sox3 / zebrafish ovaries based on RNA seq data and some of these genes were validated by qRT–PCR. Further the differentially expressed genes were devided into up regulated and down regulated genes for GO and KEGG analysis. Overall design: Ovary mRNA profiles of adult wildtype and sox3 / zebrafish were generated by deep sequencing. | pubmed:30588557 | KO mix | GSM3190267 | source name:Ovary|strain:AB|tissue:Ovary|age:Adult | KO mix | Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to GRCz10 whole genome using HISAT mapped to genes using Bowtie2. fragments per kilo bases per million fragments FPKM were calculated using RSEM. Genome build: GRCz10 Supplementary files format and content: The text files include the Ensembl ID of genes and the FPKM values for each Sample. | Ovary | Ovaries were isolated frozen on dry ice and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols. | strain:AB|tissue:Ovary|age:Adult | GSM3190267 | GSM3190267: KO mix; Danio rerio; RNA Seq | GSM3190267 | 1 | Ovaries were isolated frozen on dry ice and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols. | GEO Accession:GSM3190267 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | BGISEQ | BGISEQ-500 | SRP150521 | KO-ovary.fq.gz | fastq | 1173123100.0 | 23462462.0 | GSM3190267 r1 | 0:50 1:0 | A:308426188;C:271468505;G:292019086;T:300532126;N:677195 | 50 | 0 | 308426188 | 271468505 | 292019086 | 300532126 | 677195 | SRX4215318 | SRS3417596 | SRA721702 | GEO | Wuhan university | 1 | 0.93261 | 0.02261 | 0.76132 | 0.4585 | 50 | B | usable mapping rate | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | China | 2018-06-14 | Adult | Adult | Gonad | Reproductive System | ||||||||||||||||||
| 48813 | 48813 | SRR7341815 | SRX4215317 | SRS3417595 | SRP150521 | PRJNA476105 | Transcriptome analysis of wildtype and sox3 / zebrafish adult ovary | GSE115806 | Transcriptome Analysis | The goals of this study are to compare the differentially expressed genes between wildtype and sox3 / zebrafish ovaries based on RNA seq data and some of these genes were validated by qRT–PCR. Further the differentially expressed genes were devided into up regulated and down regulated genes for GO and KEGG analysis. Overall design: Ovary mRNA profiles of adult wildtype and sox3 / zebrafish were generated by deep sequencing. | pubmed:30588557 | WT mix | GSM3190266 | source name:Ovary|strain:AB|tissue:Ovary|age:Adult | WT mix | Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to GRCz10 whole genome using HISAT mapped to genes using Bowtie2. fragments per kilo bases per million fragments FPKM were calculated using RSEM. Genome build: GRCz10 Supplementary files format and content: The text files include the Ensembl ID of genes and the FPKM values for each Sample. | Ovary | Ovaries were isolated frozen on dry ice and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols. | strain:AB|tissue:Ovary|age:Adult | GSM3190266 | GSM3190266: WT mix; Danio rerio; RNA Seq | GSM3190266 | 1 | Ovaries were isolated frozen on dry ice and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols. | GEO Accession:GSM3190266 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | BGISEQ | BGISEQ-500 | SRP150521 | WT-ovary.fq.gz | fastq | 1176463600.0 | 23529272.0 | GSM3190266 r1 | 0:50 1:0 | A:311869864;C:270650722;G:289863261;T:303489229;N:590524 | 50 | 0 | 311869864 | 270650722 | 289863261 | 303489229 | 590524 | SRX4215317 | SRS3417595 | SRA721702 | GEO | Wuhan university | 1 | 0.93489 | 0.02765 | 0.75627 | 0.46875 | 50 | B | usable mapping rate | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | China | 2018-06-14 | Adult | Adult | Gonad | Reproductive System | ||||||||||||||||||
| 58533 | 58533 | SRR11355387 | SRX7957249 | SRS6343356 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C2 3 | C2 3 | strain:AB|isolate:C2|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 3|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C2 320200327 | C2 320200327 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C2_3_1.fq.gz C2_3_2.fq.gz | fastq fastq | 6857650800.0 | 45717672.0 | C2 3 1.fq.gz | 0:150 1:150 | A:1826466263;C:1599272942;G:1580689630;T:1851144875;N:77090 | 150 | 150 | 1826466263 | 1599272942 | 1580689630 | 1851144875 | 77090 | SRX7957249 | SRS6343356 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.93222 | 0.02854 | 0.75138 | 0.50759 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 58534 | 58534 | SRR11355388 | SRX7957248 | SRS6343355 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C2 2 | C2 2 | strain:AB|isolate:C2|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 2|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C2 220200326 | C2 220200326 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C2_2_1.fq.gz C2_2_2.fq.gz | fastq fastq | 6859954800.0 | 45733032.0 | C2 2 1.fq.gz | 0:150 1:150 | A:1836618967;C:1591135182;G:1572318102;T:1859806048;N:76501 | 150 | 150 | 1836618967 | 1591135182 | 1572318102 | 1859806048 | 76501 | SRX7957248 | SRS6343355 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.93237 | 0.03248 | 0.74663 | 0.5081 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 58535 | 58535 | SRR11355389 | SRX7957247 | SRS6343354 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C2 1 | C2 1 | strain:AB|isolate:C2|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 1|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C2 120200325 | C2 120200325 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C2_1_1.fq.gz C2_1_2.fq.gz | fastq fastq | 6618974400.0 | 44126496.0 | C2 1 1.fq.gz | 0:150 1:150 | A:1774116288;C:1533828286;G:1511526952;T:1799423408;N:79466 | 150 | 150 | 1774116288 | 1533828286 | 1511526952 | 1799423408 | 79466 | SRX7957247 | SRS6343354 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.9326 | 0.02973 | 0.75426 | 0.50678 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 58536 | 58536 | SRR11355390 | SRX7957246 | SRS6343353 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C1 3 | C1 3 | strain:AB|isolate:C1|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 3|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C1 320200324 | C1 320200324 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C1_3_1.fq.gz C1_3_2.fq.gz | fastq fastq | 6837729600.0 | 45584864.0 | C1 3 1.fq.gz | 0:150 1:150 | A:1831266308;C:1587032733;G:1565071069;T:1853950421;N:409069 | 150 | 150 | 1831266308 | 1587032733 | 1565071069 | 1853950421 | 409069 | SRX7957246 | SRS6343353 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.93541 | 0.03212 | 0.74681 | 0.50268 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 58537 | 58537 | SRR11355391 | SRX7957245 | SRS6343352 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C1 2 | C1 2 | strain:AB|isolate:C1|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 2|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C1 220200323 | C1 220200323 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C1_2_2.fq.gz C1_2_1.fq.gz | fastq fastq | 6860328000.0 | 45735520.0 | C1 2 1.fq.gz | 0:150 1:150 | A:1838176072;C:1591277709;G:1569605888;T:1861063734;N:204597 | 150 | 150 | 1838176072 | 1591277709 | 1569605888 | 1861063734 | 204597 | SRX7957245 | SRS6343352 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.93763 | 0.033 | 0.74617 | 0.50287 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 58538 | 58538 | SRR11355392 | SRX7957244 | SRS6343351 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C1 1 | C1 1 | strain:AB|isolate:C1|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 1|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C1 120200322 | C1 120200322 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C1_1_1.fq.gz C1_1_2.fq.gz | fastq fastq | 6865547700.0 | 45770318.0 | C1 1 1.fq.gz | 0:150 1:150 | A:1841774713;C:1591288582;G:1570400871;T:1861867728;N:215806 | 150 | 150 | 1841774713 | 1591288582 | 1570400871 | 1861867728 | 215806 | SRX7957244 | SRS6343351 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.93567 | 0.03152 | 0.74653 | 0.50239 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 58539 | 58539 | SRR11355393 | SRX7957243 | SRS6343350 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C0 3 | C0 3 | strain:AB|isolate:C0|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 3|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C0 320200321 | C0 320200321 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C0_3_1.fq.gz C0_3_2.fq.gz | fastq fastq | 6824622600.0 | 45497484.0 | C0 3 1.fq.gz | 0:150 1:150 | A:1833660953;C:1578510104;G:1558095174;T:1854128169;N:228200 | 150 | 150 | 1833660953 | 1578510104 | 1558095174 | 1854128169 | 228200 | SRX7957243 | SRS6343350 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.93758 | 0.0323 | 0.75024 | 0.50852 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 58540 | 58540 | SRR11355394 | SRX7957242 | SRS6343349 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C0 2 | C0 2 | strain:AB|isolate:C0|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 2|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C0 220200320 | C0 220200320 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C0_2_2.fq.gz C0_2_1.fq.gz | fastq fastq | 6819326400.0 | 45462176.0 | C0 2 1.fq.gz | 0:150 1:150 | A:1837661215;C:1571877614;G:1550458835;T:1859103408;N:225328 | 150 | 150 | 1837661215 | 1571877614 | 1550458835 | 1859103408 | 225328 | SRX7957242 | SRS6343349 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.93697 | 0.0339 | 0.74793 | 0.5035 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 58541 | 58541 | SRR11355395 | SRX7957241 | SRS6343348 | SRP253408 | PRJNA613558 | Transcriptomic assessments RNA sequencing in ovaries following treatments of gravid female zebrafish to an antibiotic mixture | PRJNA613558 | Other | As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation adult zebrafish AB strain at sexual maturity 150 dpf dpf were selected for experimentation 50% male and 50% pregnant female. Given a significant change occurred in eggs production and F1 survival at birth in present study combined with antibiotics bioaccumulated in ovary we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish and thus affect egg production and survival. Therefore ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure 0 1 and 100 ug/L using a HTS approach coupled with advanced bioinformatic tools. | C0 1 | C0 1 | strain:AB|isolate:C0|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|sex:female|tissue:ovary|collection date:2019 11 01|geo loc name:China: Shenzhen Guangdong|replicate:biological replicate 1|BioSampleModel:Model organism or animal | RNA seq of zebrafish | C0 120200319 | C0 120200319 | RNA seq of zebrafish in different conditions | RNA-Seq | TRANSCRIPTOMIC | RANDOM PCR | PAIRED | BGISEQ | BGISEQ-500 | SRP253408 | C0_1_1.fq.gz C0_1_2.fq.gz | fastq fastq | 7030804800.0 | 46872032.0 | C0 1 1.fq.gz | 0:150 1:150 | A:1891469955;C:1624531422;G:1603124564;T:1911444454;N:234405 | 150 | 150 | 1891469955 | 1624531422 | 1603124564 | 1911444454 | 234405 | SRX7957241 | SRS6343348 | SRA1056819 | Southern University of Science and Technology|School of Environmental Science and Engineering | Southern University of Science and Technology | 1 | 0.93519 | 0.0328 | 0.74602 | 0.48272 | 150 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-03-20 | Undetermined | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||
| 74160 | 74160 | SRR23576624 | SRX19463433 | SRS16856056 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T1B | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis tert no Cre R1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T1B | T1B | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T1B_1.fq.gz T1B_2.fq.gz | fastq fastq | 12301883400.0 | 123018834.0 | T1B 1.fq.gz | 0:100 1:100 | A:3241228917;C:2853095411;G:2904960052;T:3302599020;N:0 | 100 | 100 | 3241228917 | 2853095411 | 2904960052 | 3302599020 | 0 | SRX19463433 | SRS16856056 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.95272 | 0.09396 | 0.68469 | 0.54489 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74177 | 74177 | SRR23576641 | SRX19463416 | SRS16856039 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T9 | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis WT R3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T9 | T9 | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T9_1.fq.gz T9_2.fq.gz | fastq fastq | 12386772800.0 | 123867728.0 | T9 1.fq.gz | 0:100 1:100 | A:3400599530;C:2772066139;G:2813890387;T:3400216744;N:0 | 100 | 100 | 3400599530 | 2772066139 | 2813890387 | 3400216744 | 0 | SRX19463416 | SRS16856039 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.93775 | 0.12024 | 0.63838 | 0.49765 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74178 | 74178 | SRR23576642 | SRX19463415 | SRS16856037 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T8 | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis WT R2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T8 | T8 | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T8_1.fq.gz T8_2.fq.gz | fastq fastq | 12176715600.0 | 121767156.0 | T8 1.fq.gz | 0:100 1:100 | A:3287270041;C:2775700062;G:2808469679;T:3305275818;N:0 | 100 | 100 | 3287270041 | 2775700062 | 2808469679 | 3305275818 | 0 | SRX19463415 | SRS16856037 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.94261 | 0.12625 | 0.63838 | 0.49901 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74179 | 74179 | SRR23576643 | SRX19463414 | SRS16856038 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T7 | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis WT R1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T7 | T7 | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T7_1.fq.gz T7_2.fq.gz | fastq fastq | 12172655600.0 | 121726556.0 | T7 1.fq.gz | 0:100 1:100 | A:3248593263;C:2809119242;G:2855399333;T:3259543762;N:0 | 100 | 100 | 3248593263 | 2809119242 | 2855399333 | 3259543762 | 0 | SRX19463414 | SRS16856038 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.94602 | 0.10229 | 0.63603 | 0.48805 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74180 | 74180 | SRR23576644 | SRX19463413 | SRS16856036 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T6 | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis tert Cre R3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T6 | T6 | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T6_1.fq.gz T6_2.fq.gz | fastq fastq | 12378860000.0 | 123788600.0 | T6 1.fq.gz | 0:100 1:100 | A:3314969512;C:2848487369;G:2903637904;T:3311765215;N:0 | 100 | 100 | 3314969512 | 2848487369 | 2903637904 | 3311765215 | 0 | SRX19463413 | SRS16856036 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.94452 | 0.10777 | 0.62422 | 0.49742 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74181 | 74181 | SRR23576645 | SRX19463412 | SRS16856034 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T5 | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis tert Cre R2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T5 | T5 | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T5_1.fq.gz T5_2.fq.gz | fastq fastq | 12128636200.0 | 121286362.0 | T5 1.fq.gz | 0:100 1:100 | A:3365971947;C:2671909728;G:2726788212;T:3363966313;N:0 | 100 | 100 | 3365971947 | 2671909728 | 2726788212 | 3363966313 | 0 | SRX19463412 | SRS16856034 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.93852 | 0.14946 | 0.63747 | 0.52613 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74182 | 74182 | SRR23576646 | SRX19463411 | SRS16856032 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T4 | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis tert Cre R1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T4 | T4 | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T4_1.fq.gz T4_2.fq.gz | fastq fastq | 12162901400.0 | 121629014.0 | T4 1.fq.gz | 0:100 1:100 | A:3278648055;C:2767244505;G:2825703918;T:3291304922;N:0 | 100 | 100 | 3278648055 | 2767244505 | 2825703918 | 3291304922 | 0 | SRX19463411 | SRS16856032 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.94511 | 0.11642 | 0.63258 | 0.4913 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74183 | 74183 | SRR23576647 | SRX19463410 | SRS16856035 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T3 | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis tert no Cre R3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T3 | T3 | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T3_1.fq.gz T3_2.fq.gz | fastq fastq | 12163321600.0 | 121633216.0 | T3 1.fq.gz | 0:100 1:100 | A:3285334843;C:2767357208;G:2816394061;T:3294235488;N:0 | 100 | 100 | 3285334843 | 2767357208 | 2816394061 | 3294235488 | 0 | SRX19463410 | SRS16856035 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.9415 | 0.11969 | 0.62666 | 0.50292 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74184 | 74184 | SRR23576648 | SRX19463409 | SRS16856033 | SRP423783 | PRJNA937311 | Telomere elongation in the gut extends systemic healthspan of zebrafish | PRJNA937311 | Other | Telomere shortening is a hallmark of aging and is counteracted by telomerase. zebrafish gut is one of the organs with the fastest rate of telomere decline. However whether telomere dependent aging of an individual organ the gut causes systemic aging remains unknown. Here we show that preventing telomere shortening in the gut through tissue specific telomerase expression rescues premature aging of tert / . Conclusively we show that gut specific telomerase expression extends lifespan of tert / by 40 % while ameliorating natural aging. Our work demonstrates that delaying telomere shortening in the gut is sufficient to systemically counteract aging in zebrafish. | T2B | strain:AB|age:9 month|dev stage:adulte|sex:male|tissue:testis|biomaterial provider:EL MAI Mounir|birth date:2019/11/20|birth location:Nice France|treatment:testis tert no Cre R2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult male testis | T2B | T2B | RNA Seq of Danio rerio : adult male testis | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP423783 | T2B_1.fq.gz T2B_2.fq.gz | fastq fastq | 12317978600.0 | 123179786.0 | T2B 1.fq.gz | 0:100 1:100 | A:3182137355;C:2913584264;G:2974241242;T:3248015739;N:0 | 100 | 100 | 3182137355 | 2913584264 | 2974241242 | 3248015739 | 0 | SRX19463409 | SRS16856033 | SRA1593824 | Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China | Sun Yat-sen University Cancer Center Miguel Godinho Ferreira | 1 | 0.95364 | 0.08029 | 0.6882 | 0.53887 | 100 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2023-02-21 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||
| 74613 | 74613 | SRR23868264 | SRX19680348 | SRS17049789 | SRP427394 | PRJNA944944 | Comparative transcriptome analysis of testes and ovaries reveals sex biased genes and pathways in zebrafish | GSE227389 | Transcriptome Analysis | The goals of this study are to compare the differentially expressed genes between testes and ovaries of zebrafish based on RNA seq data and some of these genes were validated by qRT–PCR.Further the differentially expressed genes were devided into up regulated and down regulated genes for GO and KEGG analysis. Overall design: Testes and ovaries mRNA profiles of adult zebrafish were generated by deep sequencing. Every sample was compose of three adult individuals. | pubmed:38242380 | testes | GSM7099751 | source name:testis|tissue:testis|genotype:WT|geo loc name:missing|collection date:missing | testes | Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to GRCz10 whole genome using HISAT mapped to genes using Bowtie2. fragments per kilo bases per million fragments FPKM were calculated using RSEM. Assembly: GRCz10 Supplementary files format and content: The text files include the Ensembl ID of genes and the FPKM values for each Sample. | testis | Testes and ovaries were isolated frozen on dry ice and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols. | tissue:testis|genotype:WT | GSM7099751 | GSM7099751: testes; Danio rerio; RNA Seq | GSM7099751 r1 | GSM7099751 | 1 | Testes and ovaries were isolated frozen on dry ice and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols. | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | BGISEQ | BGISEQ-500 | SRP427394 | WT-testis.fq.gz | fastq | 1176068400.0 | 23521368.0 | GSM7099751 r1 | 0:50 | A:323799672;C:262049848;G:273563529;T:316045968;N:609383 | 50 | 323799672 | 262049848 | 273563529 | 316045968 | 609383 | SRX19680348 | SRS17049789 | SRA1687194 | Wuhan university | Wuhan university | 1 | 0.93023 | 0.11308 | 0.6462 | 0.49784 | 50 | B | usable mapping rate | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | China | 2023-03-15 | Undetermined | Undetermined | Gonad | Reproductive System | |||||||||||||||||||
| 74614 | 74614 | SRR23868265 | SRX19680347 | SRS17049788 | SRP427394 | PRJNA944944 | Comparative transcriptome analysis of testes and ovaries reveals sex biased genes and pathways in zebrafish | GSE227389 | Transcriptome Analysis | The goals of this study are to compare the differentially expressed genes between testes and ovaries of zebrafish based on RNA seq data and some of these genes were validated by qRT–PCR.Further the differentially expressed genes were devided into up regulated and down regulated genes for GO and KEGG analysis. Overall design: Testes and ovaries mRNA profiles of adult zebrafish were generated by deep sequencing. Every sample was compose of three adult individuals. | pubmed:38242380 | ovaries | GSM7099752 | source name:ovary|tissue:ovary|genotype:WT|geo loc name:missing|collection date:missing | ovaries | Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to GRCz10 whole genome using HISAT mapped to genes using Bowtie2. fragments per kilo bases per million fragments FPKM were calculated using RSEM. Assembly: GRCz10 Supplementary files format and content: The text files include the Ensembl ID of genes and the FPKM values for each Sample. | ovary | Testes and ovaries were isolated frozen on dry ice and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols. | tissue:ovary|genotype:WT | GSM7099752 | GSM7099752: ovaries; Danio rerio; RNA Seq | GSM7099752 r1 | GSM7099752 | 1 | Testes and ovaries were isolated frozen on dry ice and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols. | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | BGISEQ | BGISEQ-500 | SRP427394 | WT-ovary.fq.gz | fastq | 1176463600.0 | 23529272.0 | GSM7099752 r1 | 0:50 | A:311869864;C:270650722;G:289863261;T:303489229;N:590524 | 50 | 311869864 | 270650722 | 289863261 | 303489229 | 590524 | SRX19680347 | SRS17049788 | SRA1687194 | Wuhan university | Wuhan university | 1 | 0.93488 | 0.02755 | 0.75601 | 0.46986 | 50 | B | usable mapping rate | bgi | bgi | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | China | 2023-03-15 | Undetermined | Undetermined | Gonad | Reproductive System |
Advanced export
JSON shape: default, array, newline-delimited
CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;