run_metadata
12 rows where experiment.library_selection = "unspecified" and tissue_curation_coarse = "Sensory System"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 67683 | 67683 | SRR17235493 | SRX13414611 | SRS11316492 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 MUT 10dpf S1 | strain:Bbs1ka742|age:10dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype: / |Paired group:D|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 MUT 10dpf S1 | BBS1 MUT 10dpf S1 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_MUT_10dpf_S4_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3635985845.0 | 36892654.0 | BBS1 MUT 10dpf S4 merged.bam | 0:98.56 | A:951181543;C:805662797;G:846180468;T:1032919946;N:41091 | 98 | 951181543 | 805662797 | 846180468 | 1032919946 | 41091 | SRX13414611 | SRS11316492 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.93818 | 0.32197 | 0.69451 | 0.47394 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67684 | 67684 | SRR17235494 | SRX13414610 | SRS11316490 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 CTRL 10dpf S3 | strain:Bbs1ka742|age:10dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype:+/ |Paired group:F|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 CTRL 10dpf S3 | BBS1 CTRL 10dpf S3 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_CTRL_10dpf_S6_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3130875298.0 | 31620050.0 | BBS1 CTRL 10dpf S6 merged.bam | 0:99.02 | A:814143728;C:694726254;G:728135378;T:893834285;N:35653 | 99 | 814143728 | 694726254 | 728135378 | 893834285 | 35653 | SRX13414610 | SRS11316490 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.94014 | 0.33329 | 0.69735 | 0.47408 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67685 | 67685 | SRR17235495 | SRX13414609 | SRS11316491 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 CTRL 10dpf S2 | strain:Bbs1ka742|age:10dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype:+/ |Paired group:E|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 CTRL 10dpf S2 | BBS1 CTRL 10dpf S2 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_CTRL_10dpf_S5_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3062763746.0 | 31000736.0 | BBS1 CTRL 10dpf S5 merged.bam | 0:98.80 | A:786649223;C:679725108;G:723425238;T:872929361;N:34816 | 98 | 786649223 | 679725108 | 723425238 | 872929361 | 34816 | SRX13414609 | SRS11316491 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.94626 | 0.26532 | 0.68793 | 0.46861 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67686 | 67686 | SRR17235496 | SRX13414608 | SRS11316489 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 Ctrl 10dpf S1 | strain:Bbs1ka742|age:10dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype:+/ |Paired group:D|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 Ctrl 10dpf S1 | BBS1 Ctrl 10dpf S1 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_CTRL_10dpf_S4_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 4388386557.0 | 44385344.0 | BBS1 CTRL 10dpf S4 merged.bam | 0:98.87 | A:1141948730;C:979155774;G:1019331809;T:1247901031;N:49213 | 98 | 1141948730 | 979155774 | 1019331809 | 1247901031 | 49213 | SRX13414608 | SRS11316489 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.94326 | 0.28573 | 0.69296 | 0.47244 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67687 | 67687 | SRR17235497 | SRX13414607 | SRS11316488 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 MUT 5dpf S3 | strain:Bbs1ka742|age:5dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype: / |Paired group:C|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 MUT 5dpf S3 | BBS1 MUT 5dpf S3 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_MUT_5dpf_S3_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3030482374.0 | 30617366.0 | BBS1 MUT 5dpf S3 merged.bam | 0:98.98 | A:813926164;C:646079228;G:694857531;T:875585698;N:33753 | 98 | 813926164 | 646079228 | 694857531 | 875585698 | 33753 | SRX13414607 | SRS11316488 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.92861 | 0.41464 | 0.69051 | 0.49332 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67688 | 67688 | SRR17235498 | SRX13414606 | SRS11316487 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 MUT 5dpf S2 | strain:Bbs1ka742|age:5dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype: / |Paired group:B|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 MUT 5dpf S2 | BBS1 MUT 5dpf S2 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_MUT_5dpf_S2_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3714404169.0 | 37505417.0 | BBS1 MUT 5dpf S2 merged.bam | 0:99.04 | A:968857332;C:821571707;G:867307403;T:1056625911;N:41816 | 99 | 968857332 | 821571707 | 867307403 | 1056625911 | 41816 | SRX13414606 | SRS11316487 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.93912 | 0.32694 | 0.68781 | 0.48549 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67689 | 67689 | SRR17235499 | SRX13414605 | SRS11316486 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 MUT 5dpf S1 | strain:Bbs1ka742|age:5dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype: / |Paired group:A|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 MUT 5dpf S1 | BBS1 MUT 5dpf S1 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_MUT_5dpf_S1_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3699896449.0 | 37382583.0 | BBS1 MUT 5dpf S1 merged.bam | 0:98.97 | A:977907912;C:830475992;G:855441672;T:1036029492;N:41381 | 98 | 977907912 | 830475992 | 855441672 | 1036029492 | 41381 | SRX13414605 | SRS11316486 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.93133 | 0.40806 | 0.69562 | 0.5114 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67690 | 67690 | SRR17235500 | SRX13414604 | SRS11316485 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 CTRL 5dpf S3 | strain:Bbs1ka742|age:5dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype:+/ |Paired group:C|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 CTRL 5dpf S3 | BBS1 CTRL 5dpf S3 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_CTRL_5dpf_S3_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3505192259.0 | 35384389.0 | BBS1 CTRL 5dpf S3 merged.bam | 0:99.06 | A:910433958;C:775887812;G:822289434;T:996540997;N:40058 | 99 | 910433958 | 775887812 | 822289434 | 996540997 | 40058 | SRX13414604 | SRS11316485 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.94274 | 0.28843 | 0.68678 | 0.47383 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67691 | 67691 | SRR17235501 | SRX13414603 | SRS11316483 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 MUT 10dpf S3 | strain:Bbs1ka742|age:10dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype: / |Paired group:F|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 MUT 10dpf S3 | BBS1 MUT 10dpf S3 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_MUT_10dpf_S6_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3821277753.0 | 38662066.0 | BBS1 MUT 10dpf S6 merged.bam | 0:98.84 | A:1019329287;C:820362185;G:870436357;T:1111106893;N:43031 | 98 | 1019329287 | 820362185 | 870436357 | 1111106893 | 43031 | SRX13414603 | SRS11316483 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.93021 | 0.40772 | 0.70019 | 0.47824 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67692 | 67692 | SRR17235502 | SRX13414602 | SRS11316484 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 MUT 10dpf S2 | strain:Bbs1ka742|age:10dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype: / |Paired group:E|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 MUT 10dpf S2 | BBS1 MUT 10dpf S2 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_MUT_10dpf_S5_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 2747848263.0 | 27781273.0 | BBS1 MUT 10dpf S5 merged.bam | 0:98.91 | A:726140802;C:605265574;G:628347573;T:788063451;N:30863 | 98 | 726140802 | 605265574 | 628347573 | 788063451 | 30863 | SRX13414602 | SRS11316484 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.93216 | 0.39301 | 0.70009 | 0.50624 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67693 | 67693 | SRR17235503 | SRX13414601 | SRS11316482 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 CTRL 5dpf S2 | strain:Bbs1ka742|age:5dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype:+/ |Paired group:B|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 CTRL 5dpf S2 | BBS1 CTRL 5dpf S2 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_CTRL_5dpf_S2_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3371457502.0 | 33990750.0 | BBS1 CTRL 5dpf S2 merged.bam | 0:99.19 | A:865249225;C:752466820;G:799272800;T:954431124;N:37533 | 99 | 865249225 | 752466820 | 799272800 | 954431124 | 37533 | SRX13414601 | SRS11316482 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.94297 | 0.30382 | 0.68523 | 0.49502 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System | |||||||||||||||||||||||||||
| 67694 | 67694 | SRR17235504 | SRX13414600 | SRS11316481 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 CTRL 5dpf S1 | strain:Bbs1ka742|age:5dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype:+/ |Paired group:A|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 CTRL 5dpf S1 | BBS1 CTRL 5dpf S1 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_CTRL_5dpf_S1_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3243942424.0 | 32951170.0 | BBS1 CTRL 5dpf S1 merged.bam | 0:98.45 | A:886651528;C:694664952;G:729637645;T:932951711;N:36588 | 98 | 886651528 | 694664952 | 729637645 | 932951711 | 36588 | SRX13414600 | SRS11316481 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.92662 | 0.42838 | 0.69404 | 0.48808 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;