run_metadata
9 rows where experiment.library_selection = "unspecified", technology = "unknown" and tissue_curation_coarse = "Embryo Imprecise"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10212 | 10212 | ERR6511331 | ERX6138167 | ERS7264190 | ERP131213 | PRJEB46978 | Nano3P seq: transcriptome wide analysis of gene expression and tail dynamics using end capture nanopore sequencing | ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-12-08-2021-14:48:52:906-1159 | Other | Nano3P seq is a simple and robust method to accurately estimate transcript levels tail lengths and tail nucleotide composition information in full length individual reads with minimal library preparation biases both in the coding and non coding transcriptome. | ENA FIRST PUBLIC:2023 12 28|ENA LAST UPDATE:2023 12 28 | Zebrafish Nano3P seq of PolyA selected sample biological replicate 1 including 4 hpf RNA | Zebrafish PolyA 4 hpf | SAMEA9541420 | CENTER FOR GENOMIC REGULATION (CRG) | ENA FIRST PUBLIC:2023 12 28T01:07:23Z|ENA LAST UPDATE:2023 12 28T01:07:23Z|External Id:SAMEA9541420|INSDC center name:CENTER FOR GENOMIC REGULATION CRG|INSDC first public:2023 12 28T01:07:23Z|INSDC last update:2023 12 28T01:07:23Z|INSDC status:public|Submitter Id:Zebrafish PolyA 4 hpf|common name:zebrafish|sample name:Zebrafish PolyA 4 hpf|scientific name:Danio rerio | MinION sequencing | ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 17 08 2021 13:09:55:665 5 | cDNA8523612 | Nano3P seq | Nano3P seq | OTHER | TRANSCRIPTOMIC | unspecified | SINGLE | OXFORD_NANOPORE | MinION | ERP131213 | MinION sequencing | ENA FIRST PUBLIC:2023 12 28|ENA LAST UPDATE:2023 12 28 | zebrafish_polya_4hpf.tar.gz | nanopore | 330562220.0 | 233101.0 | ena RUN CENTER FOR GENOMIC REGULATION CRG 17 08 2021 13:09:55:665 5 | 0:1418.11 | A:86572446;C:74232962;G:69203462;T:100553350;N:0 | 1418 | 86572446 | 74232962 | 69203462 | 100553350 | 0 | ERX6138167 | ERS7264190 | ERA5757997 | CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive | CENTER FOR GENOMIC REGULATION (CRG) | 1 | 0.0 | 0.0 | 1.0 | 1536 | T | long read | ont | ont | full_length | poly_a | unknown | bulk | unknown | unknown | Spain | 2023-12-28 | Blastula | Embryo | Undetermined | Embryo Imprecise | ||||||||||||||||||||||
| 10213 | 10213 | ERR6511329 | ERX6138165 | ERS7264188 | ERP131213 | PRJEB46978 | Nano3P seq: transcriptome wide analysis of gene expression and tail dynamics using end capture nanopore sequencing | ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-12-08-2021-14:48:52:906-1159 | Other | Nano3P seq is a simple and robust method to accurately estimate transcript levels tail lengths and tail nucleotide composition information in full length individual reads with minimal library preparation biases both in the coding and non coding transcriptome. | ENA FIRST PUBLIC:2023 12 28|ENA LAST UPDATE:2023 12 28 | Zebrafish Nano3P seq of Ribodepleted sample biological replicate 1 including 2 hpf 4 hpf 6 hpf RNAs | Zebrafish Ribodep Rep1 | SAMEA9541418 | CENTER FOR GENOMIC REGULATION (CRG) | ENA FIRST PUBLIC:2023 12 28T01:07:23Z|ENA LAST UPDATE:2023 12 28T01:07:23Z|External Id:SAMEA9541418|INSDC center name:CENTER FOR GENOMIC REGULATION CRG|INSDC first public:2023 12 28T01:07:23Z|INSDC last update:2023 12 28T01:07:23Z|INSDC status:public|Submitter Id:Zebrafish Ribodep Rep1|common name:zebrafish|sample name:Zebrafish Ribodep Rep1|scientific name:Danio rerio | MinION sequencing | ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 17 08 2021 13:09:55:665 3 | cDNA786327 | Nano3P seq | Nano3P seq | OTHER | TRANSCRIPTOMIC | unspecified | SINGLE | OXFORD_NANOPORE | MinION | ERP131213 | MinION sequencing | ENA FIRST PUBLIC:2023 12 28|ENA LAST UPDATE:2023 12 28 | zebrafish_ribodep_rep1.tar.gz | nanopore | 1745399583.0 | 1644167.0 | ena RUN CENTER FOR GENOMIC REGULATION CRG 17 08 2021 13:09:55:665 3 | 0:1061.57 | A:449704009;C:421915878;G:378879857;T:494899839;N:0 | 1061 | 449704009 | 421915878 | 378879857 | 494899839 | 0 | ERX6138165 | ERS7264188 | ERA5757997 | CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive | CENTER FOR GENOMIC REGULATION (CRG) | 1 | 0.01112 | 0.0 | 0.99997 | 1.0 | 546 | T | long read | ont | ont | full_length | rrna_depletion | unknown | bulk | unknown | unknown | Spain | 2023-12-28 | Multi-stage | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||||
| 10215 | 10215 | ERR6511330 | ERX6138166 | ERS7264189 | ERP131213 | PRJEB46978 | Nano3P seq: transcriptome wide analysis of gene expression and tail dynamics using end capture nanopore sequencing | ena-STUDY-CENTER FOR GENOMIC REGULATION (CRG)-12-08-2021-14:48:52:906-1159 | Other | Nano3P seq is a simple and robust method to accurately estimate transcript levels tail lengths and tail nucleotide composition information in full length individual reads with minimal library preparation biases both in the coding and non coding transcriptome. | ENA FIRST PUBLIC:2023 12 28|ENA LAST UPDATE:2023 12 28 | Zebrafish Nano3P seq of Ribodepleted sample biological replicate 1 including 2 hpf 4 hpf 6 hpf RNAs | Zebrafish Ribodep Rep2 | SAMEA9541419 | CENTER FOR GENOMIC REGULATION (CRG) | ENA FIRST PUBLIC:2023 12 28T01:07:23Z|ENA LAST UPDATE:2023 12 28T01:07:23Z|External Id:SAMEA9541419|INSDC center name:CENTER FOR GENOMIC REGULATION CRG|INSDC first public:2023 12 28T01:07:23Z|INSDC last update:2023 12 28T01:07:23Z|INSDC status:public|Submitter Id:Zebrafish Ribodep Rep2|common name:zebrafish|sample name:Zebrafish Ribodep Rep2|scientific name:Danio rerio | MinION sequencing | ena EXPERIMENT CENTER FOR GENOMIC REGULATION CRG 17 08 2021 13:09:55:665 4 | cDNA123791 | Nano3P seq | Nano3P seq | OTHER | TRANSCRIPTOMIC | unspecified | SINGLE | OXFORD_NANOPORE | MinION | ERP131213 | MinION sequencing | ENA FIRST PUBLIC:2023 12 28|ENA LAST UPDATE:2023 12 28 | zebrafish_ribodep_rep2.tar.gz | nanopore | 2038398139.0 | 1955617.0 | ena RUN CENTER FOR GENOMIC REGULATION CRG 17 08 2021 13:09:55:665 4 | 0:1042.33 | A:518369802;C:477535545;G:441294056;T:601198736;N:0 | 1042 | 518369802 | 477535545 | 441294056 | 601198736 | 0 | ERX6138166 | ERS7264189 | ERA5757997 | CENTER FOR GENOMIC REGULATION (CRG)|European Nucleotide Archive | CENTER FOR GENOMIC REGULATION (CRG) | ont | ont | full_length | rrna_depletion | unknown | bulk | unknown | unknown | Spain | 2023-12-28 | Multi-stage | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||||||||||||
| 66222 | 66222 | SRR16148348 | SRX12433220 | SRS10400589 | SRP339629 | PRJNA767802 | RNA seq and ATAC seq of WT and Gli3 / zebrafish | PRJNA767802 | Other | WE collected EGFP cells Gli3 positive cells from CNE14:EGFP transgenic zebrafish at 55 hpf. RNA seq was performed on 3 wildtype WT and 3 Gli3 / replicates. ATAC seq was performed using EGFP cells from 3 WT replicates and using whole embryo cells from 2 WT replicates. These files reveal presumable Gli3 target genes. | Replicate 3 RNAseq Gli3 / | RNAseq Gli3KO 3 | strain:Gli3 / ex5 3|dev stage:55hpf|sex:NA|tissue:EGFP cells|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: Gli3 / EGFP cells | 6 | 6 | Total RNA was immediately extracted from isolated 100 000 EGFP positive cells by FACS using Trizol Invitrogen. Briefly the cell suspension was mixed with 1ml of Trizol by vigorous Vortexing and kept for 5 minutes at the room temperature. The mixed solution was centrifuged and the supernatant was recovered to a new tube. 0.2 ml chloroform was added vigorously vortexed and centrifuged for 15 minutes. The supernatant was mixed with 0.5 ml of isopropanol kept for 10 minutes at room temperature and centrifuged for 15 minutes. The precipitated RNA was washed by 70% ethanol and reconstituted in 30 ul water. The RNA solution was submitted to Novogene CA USA converted to sequencing library and sequenced. | RNA-Seq | TRANSCRIPTOMIC | unspecified | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP339629 | assembly:GRCz11 | Gli3KO3_aligned.sorted.bam | bam | 5067915101.0 | 25431299.0 | Gli3KO3 aligned.sorted.bam | 0:99.63 1:99.65 | A:1368455893;C:1172445678;G:1173478123;T:1353535407;N:0 | 99 | 99 | 1368455893 | 1172445678 | 1173478123 | 1353535407 | 0 | SRX12433220 | SRS10400589 | SRA1303956 | Rutgers University|Genetics | Rutgers University | 2 | 0.95469 | 0.95749 | 0.09504 | 0.09606 | 0.73951 | 0.73963 | 0.54439 | 0.54442 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | unknown | unknown | bulk | unknown | unknown | United States | 2021-10-01 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 66223 | 66223 | SRR16148349 | SRX12433219 | SRS10400587 | SRP339629 | PRJNA767802 | RNA seq and ATAC seq of WT and Gli3 / zebrafish | PRJNA767802 | Other | WE collected EGFP cells Gli3 positive cells from CNE14:EGFP transgenic zebrafish at 55 hpf. RNA seq was performed on 3 wildtype WT and 3 Gli3 / replicates. ATAC seq was performed using EGFP cells from 3 WT replicates and using whole embryo cells from 2 WT replicates. These files reveal presumable Gli3 target genes. | Replicate 2 RNAseq Gli3 / | RNAseq Gli3KO 2 | strain:Gli3 / ex5 2|dev stage:55hpf|sex:NA|tissue:EGFP cells|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: Gli3 / EGFP cells | 5 | 5 | Total RNA was immediately extracted from isolated 100 000 EGFP positive cells by FACS using Trizol Invitrogen. Briefly the cell suspension was mixed with 1ml of Trizol by vigorous Vortexing and kept for 5 minutes at the room temperature. The mixed solution was centrifuged and the supernatant was recovered to a new tube. 0.2 ml chloroform was added vigorously vortexed and centrifuged for 15 minutes. The supernatant was mixed with 0.5 ml of isopropanol kept for 10 minutes at room temperature and centrifuged for 15 minutes. The precipitated RNA was washed by 70% ethanol and reconstituted in 30 ul water. The RNA solution was submitted to Novogene CA USA converted to sequencing library and sequenced. | RNA-Seq | TRANSCRIPTOMIC | unspecified | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP339629 | assembly:GRCz11 | V300059050_Gli3KO2_aligned.cordinatesort.bam | bam | 10961381631.0 | 54976705.0 | V300059050 Gli3KO2 aligned.cordinatesort.bam | 0:99.70 1:99.68 | A:2988819475;C:2499700971;G:2467906402;T:3004531404;N:423379 | 99 | 99 | 2988819475 | 2499700971 | 2467906402 | 3004531404 | 423379 | SRX12433219 | SRS10400587 | SRA1303956 | Rutgers University|Genetics | Rutgers University | 2 | 0.94015 | 0.9439 | 0.12356 | 0.12485 | 0.75315 | 0.75304 | 0.51072 | 0.50897 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | unknown | unknown | bulk | unknown | unknown | United States | 2021-10-01 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 66224 | 66224 | SRR16148350 | SRX12433218 | SRS10400586 | SRP339629 | PRJNA767802 | RNA seq and ATAC seq of WT and Gli3 / zebrafish | PRJNA767802 | Other | WE collected EGFP cells Gli3 positive cells from CNE14:EGFP transgenic zebrafish at 55 hpf. RNA seq was performed on 3 wildtype WT and 3 Gli3 / replicates. ATAC seq was performed using EGFP cells from 3 WT replicates and using whole embryo cells from 2 WT replicates. These files reveal presumable Gli3 target genes. | Replicate 1 RNAseq Gli3 / | RNAseq Gli3KO 1 | strain:Gli3 / ex5 1|dev stage:55hpf|sex:NA|tissue:EGFP cells|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: Gli3 / EGFP cells | 4 | 4 | Total RNA was immediately extracted from isolated 100 000 EGFP positive cells by FACS using Trizol Invitrogen. Briefly the cell suspension was mixed with 1ml of Trizol by vigorous Vortexing and kept for 5 minutes at the room temperature. The mixed solution was centrifuged and the supernatant was recovered to a new tube. 0.2 ml chloroform was added vigorously vortexed and centrifuged for 15 minutes. The supernatant was mixed with 0.5 ml of isopropanol kept for 10 minutes at room temperature and centrifuged for 15 minutes. The precipitated RNA was washed by 70% ethanol and reconstituted in 30 ul water. The RNA solution was submitted to Novogene CA USA converted to sequencing library and sequenced. | RNA-Seq | TRANSCRIPTOMIC | unspecified | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP339629 | assembly:GRCz11 | V300059050_Gli3KO1_aligned.cordinatesort.bam | bam | 10204176056.0 | 51177828.0 | V300059050 Gli3KO1 aligned.cordinatesort.bam | 0:99.68 1:99.71 | A:2761524632;C:2342024573;G:2322758345;T:2777476126;N:392380 | 99 | 99 | 2761524632 | 2342024573 | 2322758345 | 2777476126 | 392380 | SRX12433218 | SRS10400586 | SRA1303956 | Rutgers University|Genetics | Rutgers University | 2 | 0.94248 | 0.9466 | 0.11206 | 0.11246 | 0.73634 | 0.73582 | 0.50038 | 0.49971 | 100 | 91 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | unknown | unknown | bulk | unknown | unknown | United States | 2021-10-01 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 66225 | 66225 | SRR16148351 | SRX12433217 | SRS10400585 | SRP339629 | PRJNA767802 | RNA seq and ATAC seq of WT and Gli3 / zebrafish | PRJNA767802 | Other | WE collected EGFP cells Gli3 positive cells from CNE14:EGFP transgenic zebrafish at 55 hpf. RNA seq was performed on 3 wildtype WT and 3 Gli3 / replicates. ATAC seq was performed using EGFP cells from 3 WT replicates and using whole embryo cells from 2 WT replicates. These files reveal presumable Gli3 target genes. | Replicate 3 RNAseq WT | RNAseq WT 3 | strain:WT3|dev stage:55hpf|sex:NA|tissue:EGFP cells|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: WT EGFP cells | 3 | 3 | Total RNA was immediately extracted from isolated 100 000 EGFP positive cells by FACS using Trizol Invitrogen. Briefly the cell suspension was mixed with 1ml of Trizol by vigorous Vortexing and kept for 5 minutes at the room temperature. The mixed solution was centrifuged and the supernatant was recovered to a new tube. 0.2 ml chloroform was added vigorously vortexed and centrifuged for 15 minutes. The supernatant was mixed with 0.5 ml of isopropanol kept for 10 minutes at room temperature and centrifuged for 15 minutes. The precipitated RNA was washed by 70% ethanol and reconstituted in 30 ul water. The RNA solution was submitted to Novogene CA USA converted to sequencing library and sequenced. | RNA-Seq | TRANSCRIPTOMIC | unspecified | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP339629 | assembly:GRCz11 | Gli3WT3_aligned.sorted.bam | bam | 6055778800.0 | 30391313.0 | Gli3WT3 aligned.sorted.bam | 0:99.60 1:99.66 | A:1632778751;C:1402895889;G:1402788250;T:1617315910;N:0 | 99 | 99 | 1632778751 | 1402895889 | 1402788250 | 1617315910 | 0 | SRX12433217 | SRS10400585 | SRA1303956 | Rutgers University|Genetics | Rutgers University | 2 | 0.95971 | 0.96273 | 0.09206 | 0.09219 | 0.72458 | 0.72391 | 0.55162 | 0.55278 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | unknown | unknown | bulk | unknown | unknown | United States | 2021-10-01 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 66226 | 66226 | SRR16148353 | SRX12433215 | SRS10400584 | SRP339629 | PRJNA767802 | RNA seq and ATAC seq of WT and Gli3 / zebrafish | PRJNA767802 | Other | WE collected EGFP cells Gli3 positive cells from CNE14:EGFP transgenic zebrafish at 55 hpf. RNA seq was performed on 3 wildtype WT and 3 Gli3 / replicates. ATAC seq was performed using EGFP cells from 3 WT replicates and using whole embryo cells from 2 WT replicates. These files reveal presumable Gli3 target genes. | Replicate 2 RNAseq WT | RNAseq WT 2 | strain:WT2|dev stage:55hpf|sex:NA|tissue:EGFP cells|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: WT EGFP cells | 2 | 2 | Total RNA was immediately extracted from isolated 100 000 EGFP positive cells by FACS using Trizol Invitrogen. Briefly the cell suspension was mixed with 1ml of Trizol by vigorous Vortexing and kept for 5 minutes at the room temperature. The mixed solution was centrifuged and the supernatant was recovered to a new tube. 0.2 ml chloroform was added vigorously vortexed and centrifuged for 15 minutes. The supernatant was mixed with 0.5 ml of isopropanol kept for 10 minutes at room temperature and centrifuged for 15 minutes. The precipitated RNA was washed by 70% ethanol and reconstituted in 30 ul water. The RNA solution was submitted to Novogene CA USA converted to sequencing library and sequenced. | RNA-Seq | TRANSCRIPTOMIC | unspecified | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP339629 | assembly:GRCz11 | Gli3WT2_aligned.sorted.bam | bam | 6030526704.0 | 30274232.0 | Gli3WT2 aligned.sorted.bam | 0:99.58 1:99.62 | A:1627670320;C:1395587022;G:1395639693;T:1611629669;N:0 | 99 | 99 | 1627670320 | 1395587022 | 1395639693 | 1611629669 | 0 | SRX12433215 | SRS10400584 | SRA1303956 | Rutgers University|Genetics | Rutgers University | 2 | 0.95942 | 0.96186 | 0.08632 | 0.08652 | 0.72671 | 0.72732 | 0.53408 | 0.55623 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | unknown | unknown | bulk | unknown | unknown | United States | 2021-10-01 | Hatching | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||
| 66227 | 66227 | SRR16148354 | SRX12433214 | SRS10400582 | SRP339629 | PRJNA767802 | RNA seq and ATAC seq of WT and Gli3 / zebrafish | PRJNA767802 | Other | WE collected EGFP cells Gli3 positive cells from CNE14:EGFP transgenic zebrafish at 55 hpf. RNA seq was performed on 3 wildtype WT and 3 Gli3 / replicates. ATAC seq was performed using EGFP cells from 3 WT replicates and using whole embryo cells from 2 WT replicates. These files reveal presumable Gli3 target genes. | Replicate 1 RNAseq WT | RNAseq WT 1 | strain:WT1|dev stage:55hpf|sex:NA|tissue:EGFP cells|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: WT EGFP cells | 1 | 1 | Total RNA was immediately extracted from isolated 100 000 EGFP positive cells by FACS using Trizol Invitrogen. Briefly the cell suspension was mixed with 1ml of Trizol by vigorous Vortexing and kept for 5 minutes at the room temperature. The mixed solution was centrifuged and the supernatant was recovered to a new tube. 0.2 ml chloroform was added vigorously vortexed and centrifuged for 15 minutes. The supernatant was mixed with 0.5 ml of isopropanol kept for 10 minutes at room temperature and centrifuged for 15 minutes. The precipitated RNA was washed by 70% ethanol and reconstituted in 30 ul water. The RNA solution was submitted to Novogene CA USA converted to sequencing library and sequenced. | RNA-Seq | TRANSCRIPTOMIC | unspecified | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP339629 | assembly:GRCz11 | V300059050_Gli3WT_aligned.cordinatesort.bam | bam | 8907848854.0 | 44671741.0 | V300059050 Gli3WT aligned.cordinatesort.bam | 0:99.68 1:99.73 | A:2444869517;C:2007841709;G:1991672704;T:2463143463;N:321461 | 99 | 99 | 2444869517 | 2007841709 | 1991672704 | 2463143463 | 321461 | SRX12433214 | SRS10400582 | SRA1303956 | Rutgers University|Genetics | Rutgers University | 2 | 0.94004 | 0.94329 | 0.13907 | 0.13978 | 0.74943 | 0.74897 | 0.507 | 0.5105 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | unknown | unknown | bulk | unknown | unknown | United States | 2021-10-01 | Hatching | Embryo | Undetermined | Embryo Imprecise |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;