run_metadata
61 rows where experiment.library_selection = "size fractionation" and experiment.library_strategy = "OTHER"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 29739 | 29739 | SRR27467672 | SRX23139234 | SRS20090275 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome mock R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf mock rep2 | EV04009 | EV04009 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04009.R1.fastq.gz | fastq | 609590940.0 | 4354221.0 | EV04009.R1.fastq.gz | 0:140 | A:155062144;C:147628702;G:168099121;T:138772205;N:28768 | 140 | 155062144 | 147628702 | 168099121 | 138772205 | 28768 | SRX23139234 | SRS20090275 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Blastula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29740 | 29740 | SRR27467673 | SRX23139233 | SRS20090278 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell BS R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf BS rep2 | EV04008 | EV04008 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04008.R1.fastq.gz | fastq | 675505180.0 | 4825037.0 | EV04008.R1.fastq.gz | 0:140 | A:178823976;C:121648962;G:198428643;T:176572292;N:31307 | 140 | 178823976 | 121648962 | 198428643 | 176572292 | 31307 | SRX23139233 | SRS20090278 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Blastula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29741 | 29741 | SRR27467674 | SRX23139232 | SRS20090272 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell DM R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf DM rep2 | EV04007 | EV04007 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04007.R1.fastq.gz | fastq | 702064440.0 | 5014746.0 | EV04007.R1.fastq.gz | 0:140 | A:176719694;C:166467163;G:207893965;T:150951950;N:31668 | 140 | 176719694 | 166467163 | 207893965 | 150951950 | 31668 | SRX23139232 | SRS20090272 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 2e-05 | 0.0 | 0.99997 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29742 | 29742 | SRR27467675 | SRX23139231 | SRS20090271 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell mock R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf mock rep2 | EV04006 | EV04006 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04006.R1.fastq.gz | fastq | 664790700.0 | 4748505.0 | EV04006.R1.fastq.gz | 0:140 | A:172014219;C:146545691;G:200358431;T:145840649;N:31710 | 140 | 172014219 | 146545691 | 200358431 | 145840649 | 31710 | SRX23139231 | SRS20090271 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 3e-05 | 0.0 | 0.99993 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29743 | 29743 | SRR27467676 | SRX23139230 | SRS20090273 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 4 cell DM R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:1 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 1 hpf DM rep2 | EV04004 | EV04004 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04004.R1.fastq.gz | fastq | 895671560.0 | 6397654.0 | EV04004.R1.fastq.gz | 0:140 | A:206443066;C:147067097;G:365386937;T:176734600;N:39860 | 140 | 206443066 | 147067097 | 365386937 | 176734600 | 39860 | SRX23139230 | SRS20090273 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Cleavage | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29744 | 29744 | SRR27467677 | SRX23139229 | SRS20090274 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 4 cell mock R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:1 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 1 hpf mock rep2 | EV04003 | EV04003 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04003.R1.fastq.gz | fastq | 664112120.0 | 4743658.0 | EV04003.R1.fastq.gz | 0:140 | A:163711695;C:142612940;G:211194350;T:146562626;N:30509 | 140 | 163711695 | 142612940 | 211194350 | 146562626 | 30509 | SRX23139229 | SRS20090274 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 2e-05 | 0.0 | 0.99997 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Cleavage | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29745 | 29745 | SRR27467678 | SRX23139228 | SRS20090268 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | eggs mock R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:activated eggs|collection date:2022|geo loc name:Austria|sex:mixed|tissue:activated eggs|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: eggs mock rep2 | EV04001 | EV04001 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04001.R1.fastq.gz | fastq | 970037880.0 | 6928842.0 | EV04001.R1.fastq.gz | 0:140 | A:258735538;C:237798398;G:268588390;T:204871983;N:43571 | 140 | 258735538 | 237798398 | 268588390 | 204871983 | 43571 | SRX23139228 | SRS20090268 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Undetermined | Embryo | Undetermined | Embryo Imprecise | ||||||||||||||||||||||||||||||
| 29746 | 29746 | SRR27467679 | SRX23139227 | SRS20090270 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary BS R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary BS rep2 | EV02003 | EV02003 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | PAIRED | ILLUMINA | NextSeq 500 | SRP482074 | EV02003.R1.fastq.gz EV02003.R2.fastq.gz | fastq fastq | 1031891720.0 | 3416860.0 | EV02003.R1.fastq.gz | 0:151 1:151 | A:264464363;C:153440613;G:408557241;T:205367487;N:62016 | 151 | 151 | 264464363 | 153440613 | 408557241 | 205367487 | 62016 | SRX23139227 | SRS20090270 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 2 | 0.0 | 0.00019 | 0.0 | 0.00015 | 1.0 | 0.99993 | 1.0 | 151 | 151 | T | T | mates < 9% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||
| 29747 | 29747 | SRR27467680 | SRX23139226 | SRS20090267 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h BS R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf BS rep2 | EV03009 | EV03009 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV03009.R1.fastq.gz | fastq | 168931280.0 | 2222780.0 | EV03009.R1.fastq.gz | 0:76 | A:49803604;C:36479774;G:37758990;T:44874154;N:14758 | 76 | 49803604 | 36479774 | 37758990 | 44874154 | 14758 | SRX23139226 | SRS20090267 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00034 | 0.0002 | 0.99965 | 0.5 | 76 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29748 | 29748 | SRR27467681 | SRX23139225 | SRS20090266 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h DM R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf DM rep2 | EV03008 | EV03008 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV03008.R1.fastq.gz | fastq | 200878792.0 | 2643142.0 | EV03008.R1.fastq.gz | 0:76 | A:46955288;C:54746275;G:55887832;T:43270561;N:18836 | 76 | 46955288 | 54746275 | 55887832 | 43270561 | 18836 | SRX23139225 | SRS20090266 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.29408 | 0.00737 | 0.91823 | 0.54549 | 76 | B | usable mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29749 | 29749 | SRR27467682 | SRX23139224 | SRS20090269 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h mock R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf mock rep2 | EV03007 | EV03007 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV03007.R1.fastq.gz | fastq | 175642840.0 | 2311090.0 | EV03007.R1.fastq.gz | 0:76 | A:44745275;C:48908904;G:44862157;T:37111895;N:14609 | 76 | 44745275 | 48908904 | 44862157 | 37111895 | 14609 | SRX23139224 | SRS20090269 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.06887 | 0.00273 | 0.96623 | 0.48721 | 76 | B | usable mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29750 | 29750 | SRR27467683 | SRX23139223 | SRS20090265 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud BS R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf BS rep2 | EV04014 | EV04014 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04014.R1.fastq.gz | fastq | 774901260.0 | 5535009.0 | EV04014.R1.fastq.gz | 0:140 | A:193865579;C:138861953;G:251102013;T:191036071;N:35644 | 140 | 193865579 | 138861953 | 251102013 | 191036071 | 35644 | SRX23139223 | SRS20090265 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Gastrula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29751 | 29751 | SRR27467684 | SRX23139222 | SRS20090264 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud DM R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf DM rep2 | EV04013 | EV04013 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04013.R1.fastq.gz | fastq | 666372840.0 | 4759806.0 | EV04013.R1.fastq.gz | 0:140 | A:172923134;C:142243998;G:211584978;T:139591020;N:29710 | 140 | 172923134 | 142243998 | 211584978 | 139591020 | 29710 | SRX23139222 | SRS20090264 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 3e-05 | 0.0 | 0.99991 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Gastrula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29752 | 29752 | SRR27467685 | SRX23139221 | SRS20090262 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud mock R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf mock rep2 | EV04012 | EV04012 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04012.R1.fastq.gz | fastq | 628769540.0 | 4491211.0 | EV04012.R1.fastq.gz | 0:140 | A:160925901;C:143204697;G:183224867;T:141385307;N:28768 | 140 | 160925901 | 143204697 | 183224867 | 141385307 | 28768 | SRX23139221 | SRS20090262 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 1e-05 | 0.0 | 0.99997 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Gastrula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29753 | 29753 | SRR27467686 | SRX23139220 | SRS20090263 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome BS R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf BS rep2 | EV04011 | EV04011 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04011.R1.fastq.gz | fastq | 720913200.0 | 5149380.0 | EV04011.R1.fastq.gz | 0:140 | A:185414155;C:126110104;G:238649631;T:170706590;N:32720 | 140 | 185414155 | 126110104 | 238649631 | 170706590 | 32720 | SRX23139220 | SRS20090263 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Blastula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29754 | 29754 | SRR27467687 | SRX23139219 | SRS20090259 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome DM R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf DM rep2 | EV04010 | EV04010 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04010.R1.fastq.gz | fastq | 443650200.0 | 3168930.0 | EV04010.R1.fastq.gz | 0:140 | A:114663509;C:102512276;G:135709753;T:90744641;N:20021 | 140 | 114663509 | 102512276 | 135709753 | 90744641 | 20021 | SRX23139219 | SRS20090259 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 1e-05 | 0.0 | 0.99997 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29755 | 29755 | SRR27467688 | SRX23139218 | SRS20090261 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary DM R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary DM rep2 | EV02002 | EV02002 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | PAIRED | ILLUMINA | NextSeq 500 | SRP482074 | EV02002.R1.fastq.gz EV02002.R2.fastq.gz | fastq fastq | 861320308.0 | 2852054.0 | EV02002.R1.fastq.gz | 0:151 1:151 | A:205625519;C:162178974;G:334206312;T:159256900;N:52603 | 151 | 151 | 205625519 | 162178974 | 334206312 | 159256900 | 52603 | SRX23139218 | SRS20090261 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 2 | 2e-05 | 0.00049 | 0.0 | 0.00027 | 1.0 | 0.99947 | 0.44444 | 151 | 151 | T | T | mates < 9% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||
| 29756 | 29756 | SRR27467689 | SRX23139217 | SRS20090260 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary mock R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary mock rep2 | EV02001 | EV02001 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | PAIRED | ILLUMINA | NextSeq 500 | SRP482074 | EV02001.R1.fastq.gz EV02001.R2.fastq.gz | fastq fastq | 520371066.0 | 1723083.0 | EV02001.R1.fastq.gz | 0:151 1:151 | A:116107273;C:90896560;G:218652568;T:94682244;N:32421 | 151 | 151 | 116107273 | 90896560 | 218652568 | 94682244 | 32421 | SRX23139217 | SRS20090260 | SRA1781872 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 2 | 2e-05 | 0.00086 | 0.0 | 0.00078 | 0.99995 | 0.99981 | 0.0 | 0.61538 | 151 | 151 | T | T | mates < 9% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-09 | Adult | Adult | Gonad | Reproductive System | ||||||||||||||||||||||
| 29757 | 29757 | SRR27437477 | SRX23109820 | SRS20064574 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell mock R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf mock rep3 | EV07007 | EV07007 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07007.R1.fastq.gz | fastq | 479994620.0 | 3428533.0 | EV07007.R1.fastq.gz | 0:140 | A:124628886;C:116843590;G:123136721;T:115372734;N:12689 | 140 | 124628886 | 116843590 | 123136721 | 115372734 | 12689 | SRX23109820 | SRS20064574 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 6e-05 | 1e-05 | 0.99989 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29758 | 29758 | SRR27437478 | SRX23109819 | SRS20064573 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 4 cell BS R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:1 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 1 hpf BS rep3 | EV07006 | EV07006 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07006.R1.fastq.gz | fastq | 2584527680.0 | 18460912.0 | EV07006.R1.fastq.gz | 0:140 | A:685107500;C:367766834;G:717359848;T:814224206;N:69292 | 140 | 685107500 | 367766834 | 717359848 | 814224206 | 69292 | SRX23109819 | SRS20064573 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 2e-05 | 1e-05 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Cleavage | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29759 | 29759 | SRR27437479 | SRX23109818 | SRS20064571 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 4 cell DM R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:1 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 1 hpf DM rep3 | EV07005 | EV07005 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07005.R1.fastq.gz | fastq | 713829480.0 | 5098782.0 | EV07005.R1.fastq.gz | 0:140 | A:183575304;C:168886779;G:213156258;T:148191838;N:19301 | 140 | 183575304 | 168886779 | 213156258 | 148191838 | 19301 | SRX23109818 | SRS20064571 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 5e-05 | 0.0 | 0.99989 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Cleavage | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29760 | 29760 | SRR27437480 | SRX23109817 | SRS20064572 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 4 cell mock R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:1 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 1 hpf mock rep3 | EV07004 | EV07004 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07004.R1.fastq.gz | fastq | 517336680.0 | 3695262.0 | EV07004.R1.fastq.gz | 0:140 | A:136783049;C:131207858;G:139873280;T:109458276;N:14217 | 140 | 136783049 | 131207858 | 139873280 | 109458276 | 14217 | SRX23109817 | SRS20064572 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 7e-05 | 0.0 | 0.99981 | 0.7 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Cleavage | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29761 | 29761 | SRR27437481 | SRX23109816 | SRS20064569 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | eggs BS R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:activated eggs|collection date:2022|geo loc name:Austria|sex:mixed|tissue:activated eggs|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: eggs BS rep3 | EV07003 | EV07003 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07003.R1.fastq.gz | fastq | 688960160.0 | 4921144.0 | EV07003.R1.fastq.gz | 0:140 | A:187831021;C:119362814;G:195214528;T:186533242;N:18555 | 140 | 187831021 | 119362814 | 195214528 | 186533242 | 18555 | SRX23109816 | SRS20064569 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Undetermined | Embryo | Undetermined | Embryo Imprecise | ||||||||||||||||||||||||||||||
| 29762 | 29762 | SRR27437482 | SRX23109815 | SRS20064568 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | eggs DM R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:activated eggs|collection date:2022|geo loc name:Austria|sex:mixed|tissue:activated eggs|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: eggs DM rep3 | EV07002 | EV07002 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07002.R1.fastq.gz | fastq | 520725380.0 | 3719467.0 | EV07002.R1.fastq.gz | 0:140 | A:138972126;C:125739732;G:142785467;T:113214166;N:13889 | 140 | 138972126 | 125739732 | 142785467 | 113214166 | 13889 | SRX23109815 | SRS20064568 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00014 | 1e-05 | 0.99969 | 0.5 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Undetermined | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||||||||||||
| 29763 | 29763 | SRR27437483 | SRX23109814 | SRS20064570 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | eggs mock R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:activated eggs|collection date:2022|geo loc name:Austria|sex:mixed|tissue:activated eggs|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: eggs mock rep3 | EV07001 | EV07001 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07001.R1.fastq.gz | fastq | 684538960.0 | 4889564.0 | EV07001.R1.fastq.gz | 0:140 | A:178906891;C:169237174;G:192342342;T:144034095;N:18458 | 140 | 178906891 | 169237174 | 192342342 | 144034095 | 18458 | SRX23109814 | SRS20064570 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0001 | 1e-05 | 0.99977 | 0.76923 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Undetermined | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||||||||||||
| 29764 | 29764 | SRR27437484 | SRX23109813 | SRS20064567 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h BS R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf BS rep3 | EV07018 | EV07018 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07018.R1.fastq.gz | fastq | 887731180.0 | 6340937.0 | EV07018.R1.fastq.gz | 0:140 | A:204102176;C:148262233;G:350287017;T:185056779;N:22975 | 140 | 204102176 | 148262233 | 350287017 | 185056779 | 22975 | SRX23109813 | SRS20064567 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29765 | 29765 | SRR27437485 | SRX23109812 | SRS20064566 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary BS R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary BS rep3 | EV04017 | EV04017 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04017.R1.fastq.gz | fastq | 576584540.0 | 4118461.0 | EV04017.R1.fastq.gz | 0:140 | A:149516980;C:91163801;G:169046762;T:166831111;N:25886 | 140 | 149516980 | 91163801 | 169046762 | 166831111 | 25886 | SRX23109812 | SRS20064566 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Adult | Adult | Gonad | Reproductive System | ||||||||||||||||||||||||||||||
| 29766 | 29766 | SRR27437486 | SRX23109811 | SRS20064565 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h DM R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf DM rep3 | EV07017 | EV07017 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07017.R1.fastq.gz | fastq | 466089960.0 | 3329214.0 | EV07017.R1.fastq.gz | 0:140 | A:117818387;C:108651920;G:144753443;T:94853810;N:12400 | 140 | 117818387 | 108651920 | 144753443 | 94853810 | 12400 | SRX23109811 | SRS20064565 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29767 | 29767 | SRR27437487 | SRX23109810 | SRS20064563 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h mock R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf mock rep3 | EV07016 | EV07016 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07016.R1.fastq.gz | fastq | 638674120.0 | 4561958.0 | EV07016.R1.fastq.gz | 0:140 | A:159572532;C:147135537;G:186885553;T:145063254;N:17244 | 140 | 159572532 | 147135537 | 186885553 | 145063254 | 17244 | SRX23109810 | SRS20064563 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 4e-05 | 0.0 | 0.99993 | 0.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29768 | 29768 | SRR27437488 | SRX23109809 | SRS20064564 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud BS R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf BS rep3 | EV07015 | EV07015 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07015.R1.fastq.gz | fastq | 598389400.0 | 4274210.0 | EV07015.R1.fastq.gz | 0:140 | A:150315140;C:106310211;G:186765596;T:154982225;N:16228 | 140 | 150315140 | 106310211 | 186765596 | 154982225 | 16228 | SRX23109809 | SRS20064564 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Gastrula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29769 | 29769 | SRR27437489 | SRX23109808 | SRS20064562 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud DM R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf DM rep3 | EV07014 | EV07014 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07014.R1.fastq.gz | fastq | 739056780.0 | 5278977.0 | EV07014.R1.fastq.gz | 0:140 | A:197948890;C:178761090;G:200265458;T:162060992;N:20350 | 140 | 197948890 | 178761090 | 200265458 | 162060992 | 20350 | SRX23109808 | SRS20064562 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 3e-05 | 0.0 | 0.99995 | 0.5 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Gastrula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29770 | 29770 | SRR27437490 | SRX23109807 | SRS20064561 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud mock R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf mock rep3 | EV07013 | EV07013 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07013.R1.fastq.gz | fastq | 844338040.0 | 6030986.0 | EV07013.R1.fastq.gz | 0:140 | A:217780710;C:182517044;G:251974206;T:192043675;N:22405 | 140 | 217780710 | 182517044 | 251974206 | 192043675 | 22405 | SRX23109807 | SRS20064561 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 4e-05 | 0.0 | 0.99993 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Gastrula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29771 | 29771 | SRR27437491 | SRX23109806 | SRS20064560 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome BS R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf BS rep3 | EV07011 | EV07011 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07011.R1.fastq.gz | fastq | 893528580.0 | 6382347.0 | EV07011.R1.fastq.gz | 0:140 | A:240101890;C:155985797;G:262065322;T:235351334;N:24237 | 140 | 240101890 | 155985797 | 262065322 | 235351334 | 24237 | SRX23109806 | SRS20064560 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Blastula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29772 | 29772 | SRR27437492 | SRX23109805 | SRS20064559 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome DM R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf DM rep3 | EV07012 | EV07012 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07012.R1.fastq.gz | fastq | 797662600.0 | 5697590.0 | EV07012.R1.fastq.gz | 0:140 | A:201908589;C:182656132;G:249672167;T:163404182;N:21530 | 140 | 201908589 | 182656132 | 249672167 | 163404182 | 21530 | SRX23109805 | SRS20064559 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 3e-05 | 0.0 | 0.99991 | 0.75 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29773 | 29773 | SRR27437493 | SRX23109804 | SRS20064558 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome mock R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf mock rep3 | EV07010 | EV07010 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07010.R1.fastq.gz | fastq | 467593280.0 | 3339952.0 | EV07010.R1.fastq.gz | 0:140 | A:122604957;C:118747514;G:124074466;T:102153374;N:12969 | 140 | 122604957 | 118747514 | 124074466 | 102153374 | 12969 | SRX23109804 | SRS20064558 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 4e-05 | 0.0 | 0.99993 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29774 | 29774 | SRR27437494 | SRX23109803 | SRS20064557 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell BS R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf BS rep3 | EV07008 | EV07008 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07008.R1.fastq.gz | fastq | 705682040.0 | 5040586.0 | EV07008.R1.fastq.gz | 0:140 | A:194506527;C:127442743;G:192893716;T:190818958;N:20096 | 140 | 194506527 | 127442743 | 192893716 | 190818958 | 20096 | SRX23109803 | SRS20064557 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Blastula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29775 | 29775 | SRR27437495 | SRX23109802 | SRS20064556 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell DM R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf DM rep3 | EV07009 | EV07009 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV07009.R1.fastq.gz | fastq | 380716280.0 | 2719402.0 | EV07009.R1.fastq.gz | 0:140 | A:95351632;C:89558330;G:115625953;T:80169603;N:10762 | 140 | 95351632 | 89558330 | 115625953 | 80169603 | 10762 | SRX23109802 | SRS20064556 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 6e-05 | 1e-05 | 0.99991 | 0.75 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29776 | 29776 | SRR27437496 | SRX23109801 | SRS20064555 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary DM R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary DM rep3 | EV04016 | EV04016 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04016.R1.fastq.gz | fastq | 635752880.0 | 4541092.0 | EV04016.R1.fastq.gz | 0:140 | A:160072248;C:143996869;G:189137330;T:142516059;N:30374 | 140 | 160072248 | 143996869 | 189137330 | 142516059 | 30374 | SRX23109801 | SRS20064555 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 1e-05 | 0.0 | 0.99997 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||||
| 29777 | 29777 | SRR27437497 | SRX23109800 | SRS20064554 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary mock R3 | strain:TLAB fish|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary mock rep3 | EV04015 | EV04015 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV04015.R1.fastq.gz | fastq | 489700680.0 | 3497862.0 | EV04015.R1.fastq.gz | 0:140 | A:126641754;C:126582886;G:130564204;T:105889815;N:22021 | 140 | 126641754 | 126582886 | 130564204 | 105889815 | 22021 | SRX23109800 | SRS20064554 | SRA1780298 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 6e-05 | 0.0 | 0.99983 | 0.55555 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-06 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||||
| 29778 | 29778 | SRR27435863 | SRX23108233 | SRS20063070 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell mock R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf mock rep4 | EV08010 | EV08010 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08010.R1.fastq.gz | fastq | 678729520.0 | 4848068.0 | EV08010.R1.fastq.gz | 0:140 | A:170672553;C:182320916;G:171865707;T:153824163;N:46181 | 140 | 170672553 | 182320916 | 171865707 | 153824163 | 46181 | SRX23108233 | SRS20063070 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0006 | 4e-05 | 0.99878 | 0.79069 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29779 | 29779 | SRR27435864 | SRX23108232 | SRS20063068 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 4 cell BS R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:1 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 1 hpf BS rep4 | EV08009 | EV08009 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08009.R1.fastq.gz | fastq | 756964460.0 | 5406889.0 | EV08009.R1.fastq.gz | 0:140 | A:191381054;C:111783194;G:185694475;T:268052883;N:52854 | 140 | 191381054 | 111783194 | 185694475 | 268052883 | 52854 | SRX23108232 | SRS20063068 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 1e-05 | 0.0 | 0.99997 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Cleavage | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29780 | 29780 | SRR27435865 | SRX23108231 | SRS20063069 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 4 cell DM R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:1 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 1 hpf DM rep4 | EV08008 | EV08008 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08008.R1.fastq.gz | fastq | 751794680.0 | 5369962.0 | EV08008.R1.fastq.gz | 0:140 | A:194845808;C:197389162;G:194749636;T:164756481;N:53593 | 140 | 194845808 | 197389162 | 194749636 | 164756481 | 53593 | SRX23108231 | SRS20063069 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00124 | 0.00029 | 0.99853 | 0.77083 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Cleavage | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29781 | 29781 | SRR27435866 | SRX23108230 | SRS20063066 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 4 cell mock R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:1 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 1 hpf mock rep4 | EV08007 | EV08007 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08007.R1.fastq.gz | fastq | 761961760.0 | 5442584.0 | EV08007.R1.fastq.gz | 0:140 | A:194053323;C:191169414;G:194452160;T:182234253;N:52610 | 140 | 194053323 | 191169414 | 194452160 | 182234253 | 52610 | SRX23108230 | SRS20063066 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00242 | 0.00062 | 0.99803 | 0.81818 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Cleavage | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29782 | 29782 | SRR27435867 | SRX23108229 | SRS20063067 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | eggs BS R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:activated eggs|collection date:2022|geo loc name:Austria|sex:mixed|tissue:activated eggs|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: eggs BS rep4 | EV08006 | EV08006 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08006.R1.fastq.gz | fastq | 501745580.0 | 3583897.0 | EV08006.R1.fastq.gz | 0:140 | A:124242793;C:76040623;G:131428076;T:170000220;N:33868 | 140 | 124242793 | 76040623 | 131428076 | 170000220 | 33868 | SRX23108229 | SRS20063067 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 4e-05 | 0.0 | 0.99993 | 0.66666 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Undetermined | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||||||||||||
| 29783 | 29783 | SRR27435868 | SRX23108228 | SRS20063063 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | eggs DM R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:activated eggs|collection date:2022|geo loc name:Austria|sex:mixed|tissue:activated eggs|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: eggs DM rep4 | EV08005 | EV08005 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08005.R1.fastq.gz | fastq | 740147520.0 | 5286768.0 | EV08005.R1.fastq.gz | 0:140 | A:192131338;C:198130943;G:182616631;T:167216834;N:51774 | 140 | 192131338 | 198130943 | 182616631 | 167216834 | 51774 | SRX23108228 | SRS20063063 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00052 | 8e-05 | 0.99922 | 0.92537 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Undetermined | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||||||||||||
| 29784 | 29784 | SRR27435869 | SRX23108227 | SRS20063065 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | eggs mock R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:activated eggs|collection date:2022|geo loc name:Austria|sex:mixed|tissue:activated eggs|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: eggs mock rep4 | EV08004 | EV08004 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08004.R1.fastq.gz | fastq | 761663140.0 | 5440451.0 | EV08004.R1.fastq.gz | 0:140 | A:194684966;C:192212418;G:196372829;T:178340184;N:52743 | 140 | 194684966 | 192212418 | 196372829 | 178340184 | 52743 | SRX23108227 | SRS20063065 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00151 | 0.00037 | 0.99884 | 0.82352 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Undetermined | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||||||||||||
| 29785 | 29785 | SRR27435870 | SRX23108226 | SRS20063064 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h BS R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf BS rep4 | EV08021 | EV08021 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08021.R1.fastq.gz | fastq | 747571860.0 | 5339799.0 | EV08021.R1.fastq.gz | 0:140 | A:185666613;C:121868156;G:181153824;T:258830310;N:52957 | 140 | 185666613 | 121868156 | 181153824 | 258830310 | 52957 | SRX23108226 | SRS20063064 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29786 | 29786 | SRR27435871 | SRX23108225 | SRS20063062 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary BS R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary BS rep4 | EV08003 | EV08003 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08003.R1.fastq.gz | fastq | 571689580.0 | 4083497.0 | EV08003.R1.fastq.gz | 0:140 | A:141414131;C:88722455;G:138790445;T:202723075;N:39474 | 140 | 141414131 | 88722455 | 138790445 | 202723075 | 39474 | SRX23108225 | SRS20063062 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 3e-05 | 0.0 | 0.99991 | 0.75 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||||
| 29787 | 29787 | SRR27435872 | SRX23108224 | SRS20063061 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h DM R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf DM rep4 | EV08020 | EV08020 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08020.R1.fastq.gz | fastq | 775090400.0 | 5536360.0 | EV08020.R1.fastq.gz | 0:140 | A:198131068;C:205114604;G:198783890;T:173006366;N:54472 | 140 | 198131068 | 205114604 | 198783890 | 173006366 | 54472 | SRX23108224 | SRS20063061 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00056 | 4e-05 | 0.9989 | 0.71052 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29788 | 29788 | SRR27435873 | SRX23108223 | SRS20063060 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h mock R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 24 hpf mock rep4 | EV08019 | EV08019 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08019.R1.fastq.gz | fastq | 763233240.0 | 5451666.0 | EV08019.R1.fastq.gz | 0:140 | A:189362337;C:199268270;G:198509945;T:176040551;N:52137 | 140 | 189362337 | 199268270 | 198509945 | 176040551 | 52137 | SRX23108223 | SRS20063060 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00036 | 6e-05 | 0.99939 | 0.85365 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29789 | 29789 | SRR27435874 | SRX23108222 | SRS20063057 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud BS R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf BS rep4 | EV08018 | EV08018 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08018.R1.fastq.gz | fastq | 809203080.0 | 5780022.0 | EV08018.R1.fastq.gz | 0:140 | A:209714496;C:130623600;G:197912909;T:270895413;N:56662 | 140 | 209714496 | 130623600 | 197912909 | 270895413 | 56662 | SRX23108222 | SRS20063057 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Gastrula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29790 | 29790 | SRR27435875 | SRX23108221 | SRS20063059 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud DM R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf DM rep4 | EV08017 | EV08017 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08017.R1.fastq.gz | fastq | 722137500.0 | 5158125.0 | EV08017.R1.fastq.gz | 0:140 | A:176408094;C:195907583;G:188487109;T:161283055;N:51659 | 140 | 176408094 | 195907583 | 188487109 | 161283055 | 51659 | SRX23108221 | SRS20063059 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00053 | 3e-05 | 0.999 | 0.70422 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Gastrula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29791 | 29791 | SRR27435876 | SRX23108220 | SRS20063058 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Bud mock R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:10 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 10 hpf mock rep4 | EV08016 | EV08016 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08016.R1.fastq.gz | fastq | 827605660.0 | 5911469.0 | EV08016.R1.fastq.gz | 0:140 | A:204173663;C:224596562;G:206999037;T:191779555;N:56843 | 140 | 204173663 | 224596562 | 206999037 | 191779555 | 56843 | SRX23108220 | SRS20063058 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00037 | 2e-05 | 0.99908 | 0.7037 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Gastrula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29792 | 29792 | SRR27435877 | SRX23108219 | SRS20063056 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome BS R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf BS rep4 | EV08015 | EV08015 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08015.R1.fastq.gz | fastq | 804598200.0 | 5747130.0 | EV08015.R1.fastq.gz | 0:140 | A:215467427;C:134937180;G:189531758;T:264604493;N:57342 | 140 | 215467427 | 134937180 | 189531758 | 264604493 | 57342 | SRX23108219 | SRS20063056 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 2e-05 | 0.0 | 0.99995 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29793 | 29793 | SRR27435878 | SRX23108218 | SRS20063055 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome DM R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf DM rep4 | EV08014 | EV08014 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08014.R1.fastq.gz | fastq | 662443740.0 | 4731741.0 | EV08014.R1.fastq.gz | 0:140 | A:164941700;C:167025964;G:177255084;T:153175163;N:45829 | 140 | 164941700 | 167025964 | 177255084 | 153175163 | 45829 | SRX23108218 | SRS20063055 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00069 | 0.0001 | 0.9992 | 0.83076 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29794 | 29794 | SRR27435879 | SRX23108217 | SRS20063052 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | Dome mock R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:5 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 5 hpf mock rep4 | EV08013 | EV08013 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08013.R1.fastq.gz | fastq | 794760540.0 | 5676861.0 | EV08013.R1.fastq.gz | 0:140 | A:197361776;C:210230329;G:205734036;T:181380205;N:54194 | 140 | 197361776 | 210230329 | 205734036 | 181380205 | 54194 | SRX23108217 | SRS20063052 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00066 | 9e-05 | 0.99894 | 0.78378 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29795 | 29795 | SRR27435880 | SRX23108216 | SRS20063054 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell BS R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:BS|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf BS rep4 | EV08012 | EV08012 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08012.R1.fastq.gz | fastq | 722593200.0 | 5161380.0 | EV08012.R1.fastq.gz | 0:140 | A:188475497;C:119550806;G:173208825;T:241306038;N:52034 | 140 | 188475497 | 119550806 | 173208825 | 241306038 | 52034 | SRX23108216 | SRS20063054 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.0 | 0.0 | 1.0 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Blastula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||
| 29796 | 29796 | SRR27435881 | SRX23108215 | SRS20063053 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 1K cell DM R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:3 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: embryo 3 hpf DM rep4 | EV08011 | EV08011 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08011.R1.fastq.gz | fastq | 753783380.0 | 5384167.0 | EV08011.R1.fastq.gz | 0:140 | A:190029713;C:193734250;G:194752855;T:175212145;N:54417 | 140 | 190029713 | 193734250 | 194752855 | 175212145 | 54417 | SRX23108215 | SRS20063053 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00076 | 5e-05 | 0.99859 | 0.70873 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29797 | 29797 | SRR27435882 | SRX23108214 | SRS20063050 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary DM R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:DM|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary DM rep4 | EV08002 | EV08002 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08002.R1.fastq.gz | fastq | 668593800.0 | 4775670.0 | EV08002.R1.fastq.gz | 0:140 | A:164584224;C:175789134;G:175538168;T:152637106;N:45168 | 140 | 164584224 | 175789134 | 175538168 | 152637106 | 45168 | SRX23108214 | SRS20063050 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00525 | 2e-05 | 0.99192 | 0.63501 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||||
| 29798 | 29798 | SRR27435883 | SRX23108213 | SRS20063051 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | ovary mock R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|treatment:mock|BioSampleModel:Model organism or animal | tRAM seq of zebrafish: ovary mock rep4 | EV08001 | EV08001 | RNA was extracted with Trizol tRNA isolated by size selection on denaturing polyacrylamide gel range 50 150 nt. The RNA was end repaired by alkaline deacylation and T4 PNK treatment three prime adapter ligated with T4 RNA ligase 2 reverse transcribed with TGIRT. The cDNA was circularized with CircLigase and amplified with KAPA HiFi polymerase using NEB Next indexed primers. | OTHER | TRANSCRIPTOMIC | size fractionation | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV08001.R1.fastq.gz | fastq | 753247460.0 | 5380339.0 | EV08001.R1.fastq.gz | 0:140 | A:183862675;C:196453674;G:197604061;T:175275086;N:51964 | 140 | 183862675 | 196453674 | 197604061 | 175275086 | 51964 | SRX23108213 | SRS20063051 | SRA1780265 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.00754 | 4e-05 | 0.98957 | 0.51048 | 140 | T | under 1.2% mapping rate | illumina | nextseq | unknown | size_fractionation | nebnext | bulk | unknown | unknown | Austria | 2024-01-05 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||||||||||
| 40707 | 40707 | SRR3502888 | SRX1760541 | SRS1436386 | SRP072296 | PRJNA316313 | Codon optimality and mRNA decay in zebrafish and Xenopus | PRJNA316313 | Other | Cellular transitions require dramatic changes in gene expression that are supported by regulated mRNA decay and new transcription. The maternal to zygotic transition is a conserved developmental progression during which thousands of maternal mRNAs are cleared by posttranscriptional mechanisms. Although some maternal mRNAs are targeted for degradation by microRNAs this pathway does not fully explain mRNA clearance. Because the ribosome constitutes the main ribonucleoprotein complex decoding the mRNA we investigated how codon identity and translation affect mRNA stability during development and homeostasis. Using an in vivo selection strategy we show that the codon triplet contains translation dependent regulatory information that influences transcript decay. We find that codon composition shapes maternal mRNA clearance during the maternal to zygotic transition in zebrafish Xenopus mouse and Drosophila and gene expression during homeostasis across human tissues. Codon composition affects both polyadenylation status and translation efficiency. Thus the ribosome interprets two codes within the mRNA the genetic code which specifies the amino acid sequence and a conserved “codon optimality code” that shapes mRNA stability and translation efficiency across vertebrates. | Zebrafish tRNA at 6hpf | tRNA AB 6h | strain:TUAB|age:6hpf|dev stage:Shield|sex:pooled male and female|tissue:Whole animal|collected by:Ariel Bazzini|genotype:WT|BioSampleModel:Model organism or animal | Zebrafish tRNAs at 6hpf | tRNA 6hpf zebrafish | TGIRT tRNA Library | 1 | OTHER | TRANSCRIPTOMIC | size fractionation | PAIRED | ILLUMINA | Illumina HiSeq 2500 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>152</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>77</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP072296 | AB-tRNA_S9_R1_001.fastq.gz AB-tRNA_S9_R2_001.fastq.gz | fastq fastq | 7860522000.0 | 26201740.0 | tRNA zebrafish 6hpf | 0:150 1:150 | A:1945184302;C:2012637521;G:2171934357;T:1730625218;N:140602 | 150 | 150 | 1945184302 | 2012637521 | 2171934357 | 1730625218 | 140602 | SRX1760541 | SRS1436386 | SRA395141 | Yale University|Giraldez Lab | Yale University | 2 | 0.04186 | 0.04313 | 0.00633 | 0.00639 | 0.96834 | 0.96913 | 0.53967 | 0.5329 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | size_fractionation | unknown | bulk | unknown | unknown | United States | 2016-06-17 | Gastrula | Embryo | Whole Organism | All anatomical structures |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;