metadata: run_metadata: 49 rows where experiment.library_selection = "other", experiment.library_strategy = "RNA-Seq" and tissue

49 rows where experiment.library_selection = "other", experiment.library_strategy = "RNA-Seq" and tissue_curation = "Kidney"

descending

Link	rowid ▼	run.accession	experiment.accession	sample.accession	study.accession	bioproject	study.title	study.alias	study.type	study.abstract	sample.title	sample.alias	sample.attributes	experiment.title	experiment.alias	experiment.library_name	experiment.design_description	experiment.library_strategy	experiment.library_source	experiment.library_selection	experiment.library_layout	experiment.platform	experiment.instrument_model	experiment.study_ref	run.filename	run.semantic_name	run.total_bases	run.total_spots	run.alias	run.read_lengths	run.base_counts	run.r1_length	run.r2_length	run.Acount	run.Ccount	run.Gcount	run.Tcount	run.Ncount	run.experiment	run.pool_member	submission.accession	submission.srasource	submission.bioprojectsource	seqdetective.n_mates	seqdetective.mapping_rate.mate1	seqdetective.mapping_rate.mate2	seqdetective.nofeature_rate.mate1	seqdetective.nofeature_rate.mate2	seqdetective.sparsity.mate1	seqdetective.sparsity.mate2	seqdetective.pos_strand_rate.mate1	seqdetective.pos_strand_rate.mate2	seqdetective.readlen.mate1	seqdetective.readlen.mate2	seqdetective.judgement.mate1	seqdetective.judgement.mate2	seqdetective.judgement.reason	platform_family	instrument_generation	read_bias	selection_class	prep_kit	sc_or_bulk	tech_class	technology	submission.bioprojectsource.country	earliest_date	devstage_curation	devstage_curation_coarse	tissue_curation	tissue_curation_coarse
53291	53291	SRR9849281	SRX6603894	SRS5168943	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 5 mpf replicate 5	wt 14560 b kidney	strain:AB\|age:5months\|sex:female\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 5\|BioSampleModel:Model organism or animal	wildtype kidney 5 mpf replicate 5	wt 14560 b kidney	wt 14560 b kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-b-kidney_merged_S17-1.fastq.gz wt-14560-b-kidney_merged_S17-2.fastq.gz	fastq fastq	3653995129.0	24359221.0	wt 14560 b kidney merged S17 1.fastq.gz	0:75.01 1:74.99	A:929157328;C:876547415;G:871160744;T:954251039;N:22878603	75	74	929157328	876547415	871160744	954251039	22878603	SRX6603894	SRS5168943	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95086	0.95525	0.0929	0.09179	0.72099	0.72226	0.54133	0.53922	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53292	53292	SRR9849193	SRX6603893	SRS5168942	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 5 mpf replicate 4	wt 14560 g kidney	strain:AB\|age:5months\|sex:male\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 4\|BioSampleModel:Model organism or animal	wildtype kidney 5 mpf replicate 4	wt 14560 g kidney	wt 14560 g kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-g-kidney_merged_S77-1.fastq.gz wt-14560-g-kidney_merged_S77-2.fastq.gz	fastq fastq	3573615196.0	24717802.0	wt 14560 g kidney merged S77 1.fastq.gz	0:72.27 1:72.31	A:898296425;C:813436864;G:804954936;T:918903824;N:138023147	72	72	898296425	813436864	804954936	918903824	138023147	SRX6603893	SRS5168942	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95022	0.95352	0.10595	0.10566	0.7149	0.71589	0.55515	0.55564	35	35	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53293	53293	SRR9849199	SRX6603892	SRS5168941	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 3 mpf replicate 3	kl 14744 g kidney	strain:AB\|age:3months\|sex:male\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 3\|BioSampleModel:Model organism or animal	Klotho kidney 3 mpf replicate 3	kl 14744 g kidney	kl 14744 g kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-g-kidney_merged_S83-1.fastq.gz kl-14744-g-kidney_merged_S83-2.fastq.gz	fastq fastq	3737995505.0	25138545.0	kl 14744 g kidney merged S83 1.fastq.gz	0:74.34 1:74.35	A:957928510;C:878559278;G:871082168;T:980068971;N:50356578	74	74	957928510	878559278	871082168	980068971	50356578	SRX6603892	SRS5168941	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94562	0.94982	0.09916	0.09805	0.71585	0.71727	0.53455	0.52965	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53294	53294	SRR9849194	SRX6603891	SRS5168940	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 3 mpf replicate 2	kl 14744 e kidney	strain:AB\|age:3months\|sex:male\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 2\|BioSampleModel:Model organism or animal	Klotho kidney 3 mpf replicate 2	kl 14744 e kidney	kl 14744 e kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-e-kidney_merged_S59-1.fastq.gz kl-14744-e-kidney_merged_S59-2.fastq.gz	fastq fastq	3189473322.0	21535442.0	kl 14744 e kidney merged S59 1.fastq.gz	0:74.05 1:74.05	A:823511818;C:738062888;G:732498035;T:841940599;N:53459982	74	74	823511818	738062888	732498035	841940599	53459982	SRX6603891	SRS5168940	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94678	0.95071	0.09344	0.09218	0.71971	0.72129	0.54757	0.54618	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53295	53295	SRR9849198	SRX6603890	SRS5168939	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 3 mpf replicate 1	kl 14744 a kidney	strain:AB\|age:3months\|sex:male\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 1\|BioSampleModel:Model organism or animal	Klotho kidney 3 mpf replicate 1	kl 14744 a kidney	kl 14744 a kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-a-kidney_merged_S58-1.fastq.gz kl-14744-a-kidney_merged_S58-2.fastq.gz	fastq fastq	3553107262.0	24182301.0	kl 14744 a kidney merged S58 1.fastq.gz	0:73.47 1:73.46	A:902558501;C:824321758;G:817836028;T:922899545;N:85491430	73	73	902558501	824321758	817836028	922899545	85491430	SRX6603890	SRS5168939	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95045	0.95329	0.09674	0.09571	0.72072	0.72135	0.52747	0.53937	74	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53296	53296	SRR9849195	SRX6603889	SRS5168938	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 5 mpf replicate 8	wt 14560 h kidney	strain:AB\|age:5months\|sex:female\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 8\|BioSampleModel:Model organism or animal	wildtype kidney 5 mpf replicate 8	wt 14560 h kidney	wt 14560 h kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-h-kidney_merged_S89-1.fastq.gz wt-14560-h-kidney_merged_S89-2.fastq.gz	fastq fastq	3309513073.0	22153246.0	wt 14560 h kidney merged S89 1.fastq.gz	0:74.72 1:74.67	A:790892918;C:836461180;G:837452371;T:813600384;N:31106220	74	74	790892918	836461180	837452371	813600384	31106220	SRX6603889	SRS5168938	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.96472	0.96761	0.054	0.05438	0.71175	0.71348	0.51051	0.51195	75	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53297	53297	SRR9849196	SRX6603888	SRS5168937	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 3 mpf replicate 4	kl 14744 b kidney	strain:AB\|age:3months\|sex:female\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 4\|BioSampleModel:Model organism or animal	Klotho kidney 3 mpf replicate 4	kl 14744 b kidney	kl 14744 b kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-b-kidney_merged_S70-1.fastq.gz kl-14744-b-kidney_merged_S70-2.fastq.gz	fastq fastq	3696398655.0	24993764.0	kl 14744 b kidney merged S70 1.fastq.gz	0:73.95 1:73.94	A:928283187;C:880520024;G:868819514;T:952460036;N:66315894	73	73	928283187	880520024	868819514	952460036	66315894	SRX6603888	SRS5168937	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94725	0.95062	0.08433	0.08251	0.7139	0.71334	0.52496	0.52625	65	65	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53298	53298	SRR9849197	SRX6603887	SRS5168935	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 3 mpf replicate 5	kl 14744 d kidney	strain:AB\|age:3months\|sex:female\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 5\|BioSampleModel:Model organism or animal	Klotho kidney 3 mpf replicate 5	kl 14744 d kidney	kl 14744 d kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-d-kidney_merged_S94-1.fastq.gz kl-14744-d-kidney_merged_S94-2.fastq.gz	fastq fastq	3235564566.0	21704627.0	kl 14744 d kidney merged S94 1.fastq.gz	0:74.55 1:74.53	A:808568448;C:783575935;G:776803253;T:830140556;N:36476374	74	74	808568448	783575935	776803253	830140556	36476374	SRX6603887	SRS5168935	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95065	0.95457	0.07932	0.0782	0.69844	0.69852	0.52161	0.52384	75	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53299	53299	SRR9849200	SRX6603886	SRS5168936	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 5 mpf replicate 7	kl 14563 b kidney	strain:AB\|age:5months\|sex:female\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 7\|BioSampleModel:Model organism or animal	Klotho kidney 5 mpf replicate 7	kl 14563 b kidney	kl 14563 b kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14563-b-kidney_merged_S23-1.fastq.gz kl-14563-b-kidney_merged_S23-2.fastq.gz	fastq fastq	3841497499.0	25646923.0	kl 14563 b kidney merged S23 1.fastq.gz	0:74.90 1:74.89	A:999048603;C:899322616;G:891418096;T:1023530696;N:28177488	74	74	999048603	899322616	891418096	1023530696	28177488	SRX6603886	SRS5168936	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94562	0.94897	0.098	0.09621	0.71977	0.7209	0.54321	0.53814	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53300	53300	SRR9849206	SRX6603885	SRS5168934	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 5 mpf replicate 8	kl 14563 c kidney	strain:AB\|age:5months\|sex:female\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 8\|BioSampleModel:Model organism or animal	Klotho kidney 5 mpf replicate 8	kl 14563 c kidney	kl 14563 c kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14563-c-kidney_merged_S35-1.fastq.gz kl-14563-c-kidney_merged_S35-2.fastq.gz	fastq fastq	4215569581.0	28089663.0	kl 14563 c kidney merged S35 1.fastq.gz	0:75.05 1:75.03	A:1122288966;C:970063144;G:955510223;T:1146500593;N:21206655	75	75	1122288966	970063144	955510223	1146500593	21206655	SRX6603885	SRS5168934	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94171	0.94373	0.11361	0.11133	0.70136	0.7025	0.49345	0.51472	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53301	53301	SRR9849201	SRX6603884	SRS5168933	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 5 mpf replicate 5	kl 14562 b kidney	strain:AB\|age:5months\|sex:female\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 5\|BioSampleModel:Model organism or animal	Klotho kidney 5 mpf replicate 5	kl 14562 b kidney	kl 14562 b kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14562-b-kidney_merged_S18-1.fastq.gz kl-14562-b-kidney_merged_S18-2.fastq.gz	fastq fastq	3972193490.0	27155081.0	kl 14562 b kidney merged S18 1.fastq.gz	0:73.14 1:73.14	A:987358107;C:933387787;G:924448548;T:1015576411;N:111422637	73	73	987358107	933387787	924448548	1015576411	111422637	SRX6603884	SRS5168933	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94949	0.95176	0.08272	0.08121	0.7166	0.71705	0.53783	0.53989	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53302	53302	SRR9849205	SRX6603883	SRS5168932	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 5 mpf replicate 6	kl 14562 d kidney	strain:AB\|age:5months\|sex:female\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 6\|BioSampleModel:Model organism or animal	Klotho kidney 5 mpf replicate 6	kl 14562 d kidney	kl 14562 d kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14562-d-kidney_merged_S42-1.fastq.gz kl-14562-d-kidney_merged_S42-2.fastq.gz	fastq fastq	3526368194.0	23516128.0	kl 14562 d kidney merged S42 1.fastq.gz	0:75.00 1:74.95	A:869319867;C:871562247;G:869066601;T:893717214;N:22702265	75	74	869319867	871562247	869066601	893717214	22702265	SRX6603883	SRS5168932	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.96175	0.96369	0.06361	0.06218	0.70905	0.71157	0.51899	0.52384	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53303	53303	SRR9849202	SRX6603882	SRS5168931	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 5 mpf replicate 3	kl 14563 a kidney	strain:AB\|age:5months\|sex:male\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 3\|BioSampleModel:Model organism or animal	Klotho kidney 5 mpf replicate 3	kl 14563 a kidney	kl 14563 a kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14563-a-kidney_merged_S11-1.fastq.gz kl-14563-a-kidney_merged_S11-2.fastq.gz	fastq fastq	4326402378.0	29503938.0	kl 14563 a kidney merged S11 1.fastq.gz	0:73.31 1:73.33	A:1085969692;C:1017480039;G:1010619557;T:1105911425;N:106421665	73	73	1085969692	1017480039	1010619557	1105911425	106421665	SRX6603882	SRS5168931	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94169	0.94864	0.08735	0.0856	0.74018	0.74063	0.532	0.54233	35	35	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53304	53304	SRR9849203	SRX6603881	SRS5168930	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 5 mpf replicate 1	kl 14562 a kidney	strain:AB\|age:5months\|sex:male\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 1\|BioSampleModel:Model organism or animal	Klotho kidney 5 mpf replicate 1	kl 14562 a kidney	kl 14562 a kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14562-a-kidney_merged_S6-1.fastq.gz kl-14562-a-kidney_merged_S6-2.fastq.gz	fastq fastq	4980501349.0	34004455.0	kl 14562 a kidney merged S6 1.fastq.gz	0:73.23 1:73.24	A:1251556852;C:1167350759;G:1159943717;T:1277350171;N:124299850	73	73	1251556852	1167350759	1159943717	1277350171	124299850	SRX6603881	SRS5168930	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94619	0.94971	0.08823	0.08683	0.72807	0.72796	0.51972	0.52145	35	35	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53305	53305	SRR9849204	SRX6603880	SRS5168929	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 5 mpf replicate 4	kl 14563 d kidney	strain:AB\|age:5months\|sex:male\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 4\|BioSampleModel:Model organism or animal	Klotho kidney 5 mpf replicate 4	kl 14563 d kidney	kl 14563 d kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14563-d-kidney_merged_S47-1.fastq.gz kl-14563-d-kidney_merged_S47-2.fastq.gz	fastq fastq	6869441307.0	56338972.0	kl 14563 d kidney merged S47 1.fastq.gz	0:60.76 1:61.17	A:1348721440;C:1368998259;G:1366726420;T:1380954564;N:1404040624	60	61	1348721440	1368998259	1366726420	1380954564	1404040624	SRX6603880	SRS5168929	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.96257	0.96453	0.06751	0.06671	0.75548	0.75607	0.51978	0.52832	74	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53306	53306	SRR9849207	SRX6603879	SRS5168928	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 5 mpf replicate 2	kl 14562 c kidney	strain:AB\|age:5months\|sex:male\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 2\|BioSampleModel:Model organism or animal	Klotho kidney 5 mpf replicate 2	kl 14562 c kidney	kl 14562 c kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14562-c-kidney_merged_S30-1.fastq.gz kl-14562-c-kidney_merged_S30-2.fastq.gz	fastq fastq	3320801196.0	22339756.0	kl 14562 c kidney merged S30 1.fastq.gz	0:74.33 1:74.32	A:856878404;C:774178244;G:769653230;T:875894981;N:44196337	74	74	856878404	774178244	769653230	875894981	44196337	SRX6603879	SRS5168928	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95411	0.95756	0.08333	0.08245	0.7162	0.71638	0.56576	0.55653	74	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53307	53307	SRR9849213	SRX6603878	SRS5168927	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 3 mpf replicate 6	kl 14744 f kidney	strain:AB\|age:3months\|sex:female\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 6\|BioSampleModel:Model organism or animal	Klotho kidney 3 mpf replicate 6	kl 14744 f kidney	kl 14744 f kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-f-kidney_merged_S71-1.fastq.gz kl-14744-f-kidney_merged_S71-2.fastq.gz	fastq fastq	2801226765.0	18731091.0	kl 14744 f kidney merged S71 1.fastq.gz	0:74.79 1:74.76	A:720950274;C:664580809;G:653567624;T:738645101;N:23482957	74	74	720950274	664580809	653567624	738645101	23482957	SRX6603878	SRS5168927	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94692	0.95016	0.10024	0.09917	0.69781	0.69745	0.53063	0.53615	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53308	53308	SRR9849208	SRX6603877	SRS5168926	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho kidney 3 mpf replicate 7	kl 14744 h kidney	strain:AB\|age:3months\|sex:female\|tissue:kidney\|genotype:aKlotho\|Replicate:Bilogical replicate 7\|BioSampleModel:Model organism or animal	Klotho kidney 3 mpf replicate 7	kl 14744 h kidney	kl 14744 h kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-h-kidney_merged_S95-1.fastq.gz kl-14744-h-kidney_merged_S95-2.fastq.gz	fastq fastq	3606112938.0	24298531.0	kl 14744 h kidney merged S95 1.fastq.gz	0:74.21 1:74.20	A:907594636;C:863534560;G:852256795;T:929423242;N:53303705	74	74	907594636	863534560	852256795	929423242	53303705	SRX6603877	SRS5168926	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95343	0.95612	0.07895	0.07817	0.70944	0.71023	0.54468	0.54382	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53309	53309	SRR9849212	SRX6603876	SRS5168925	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 5 mpf replicate 2	wt 14560 c kidney	strain:AB\|age:5months\|sex:male\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 2\|BioSampleModel:Model organism or animal	wildtype kidney 5 mpf replicate 2	wt 14560 c kidney	wt 14560 c kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-c-kidney_merged_S29-1.fastq.gz wt-14560-c-kidney_merged_S29-2.fastq.gz	fastq fastq	3091657773.0	21774524.0	wt 14560 c kidney merged S29 1.fastq.gz	0:70.96 1:71.03	A:763113194;C:693515052;G:687380502;T:778507279;N:169141746	70	71	763113194	693515052	687380502	778507279	169141746	SRX6603876	SRS5168925	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95062	0.95349	0.09247	0.09119	0.72754	0.72863	0.53643	0.54181	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53310	53310	SRR9849209	SRX6603875	SRS5168924	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 5 mpf replicate 3	wt 14560 e kidney	strain:AB\|age:5months\|sex:male\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 3\|BioSampleModel:Model organism or animal	wildtype kidney 5 mpf replicate 3	wt 14560 e kidney	wt 14560 e kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-e-kidney_merged_S53-1.fastq.gz wt-14560-e-kidney_merged_S53-2.fastq.gz	fastq fastq	3242753931.0	21639832.0	wt 14560 e kidney merged S53 1.fastq.gz	0:74.93 1:74.92	A:843085674;C:760074682;G:756945733;T:860752379;N:21895463	74	74	843085674	760074682	756945733	860752379	21895463	SRX6603875	SRS5168924	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95058	0.95272	0.0982	0.09719	0.71707	0.71825	0.5497	0.53642	75	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53311	53311	SRR9849210	SRX6603874	SRS5168923	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 3 mpf replicate 7	wt 14746 g kidney	strain:AB\|age:3months\|sex:female\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 7\|BioSampleModel:Model organism or animal	wildtype kidney 3 mpf replicate 7	wt 14746 g kidney	wt 14746 g kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-g-kidney_merged_S22-1.fastq.gz wt-14746-g-kidney_merged_S22-2.fastq.gz	fastq fastq	3928427553.0	26318173.0	wt 14746 g kidney merged S22 1.fastq.gz	0:74.63 1:74.64	A:1019611627;C:916883865;G:906743219;T:1044351913;N:40836929	74	74	1019611627	916883865	906743219	1044351913	40836929	SRX6603874	SRS5168923	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94281	0.94563	0.09937	0.0975	0.71478	0.71585	0.54524	0.5444	76	74	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53312	53312	SRR9849211	SRX6603873	SRS5168922	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 3 mpf replicate 6	wt 14746 d kidney	strain:AB\|age:3months\|sex:female\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 6\|BioSampleModel:Model organism or animal	wildtype kidney 3 mpf replicate 6	wt 14746 d kidney	wt 14746 d kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-d-kidney_merged_S81-2.fastq.gz wt-14746-d-kidney_merged_S81-1.fastq.gz	fastq fastq	3235839633.0	21863802.0	wt 14746 d kidney merged S81 1.fastq.gz	0:73.99 1:74.01	A:819433052;C:762877585;G:759574301;T:837534821;N:56419874	73	74	819433052	762877585	759574301	837534821	56419874	SRX6603873	SRS5168922	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94986	0.95295	0.09789	0.09661	0.72109	0.72344	0.5002	0.53439	75	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53313	53313	SRR9849214	SRX6603872	SRS5168921	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 3 mpf replicate 8	wt 14746 i kidney	strain:AB\|age:3months\|sex:female\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 8\|BioSampleModel:Model organism or animal	wildtype kidney 3 mpf replicate 8	wt 14746 i kidney	wt 14746 i kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-i-kidney_merged_S46-1.fastq.gz wt-14746-i-kidney_merged_S46-2.fastq.gz	fastq fastq	3377453037.0	23023990.0	wt 14746 i kidney merged S46 1.fastq.gz	0:73.35 1:73.34	A:836450075;C:803489724;G:795497926;T:858148995;N:83866317	73	73	836450075	803489724	795497926	858148995	83866317	SRX6603872	SRS5168921	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95192	0.95558	0.0795	0.07926	0.70589	0.70619	0.52743	0.52432	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53314	53314	SRR9849220	SRX6603871	SRS5168920	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 5 mpf replicate 1	wt 14560 a kidney	strain:AB\|age:5months\|sex:male\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 1\|BioSampleModel:Model organism or animal	wildtype kidney 5 mpf replicate 1	wt 14560 a kidney	wt 14560 a kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-a-kidney_merged_S5-1.fastq.gz wt-14560-a-kidney_merged_S5-2.fastq.gz	fastq fastq	3803097028.0	29032173.0	wt 14560 a kidney merged S5 1.fastq.gz	0:65.34 1:65.66	A:880043540;C:764446357;G:759630921;T:898342437;N:500633773	65	65	880043540	764446357	759630921	898342437	500633773	SRX6603871	SRS5168920	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94594	0.94896	0.10801	0.10661	0.74292	0.74379	0.56139	0.56212	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53315	53315	SRR9849215	SRX6603870	SRS5168919	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 3 mpf replicate 2	wt 14746 e kidney	strain:AB\|age:3months\|sex:male\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 2\|BioSampleModel:Model organism or animal	wildtype kidney 3 mpf replicate 2	wt 14746 e kidney	wt 14746 e kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-e-kidney_merged_S93-1.fastq.gz wt-14746-e-kidney_merged_S93-2.fastq.gz	fastq fastq	3389715259.0	23030176.0	wt 14746 e kidney merged S93 1.fastq.gz	0:73.58 1:73.60	A:845566798;C:806510240;G:801049741;T:861758601;N:74829879	73	73	845566798	806510240	801049741	861758601	74829879	SRX6603870	SRS5168919	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.96201	0.96536	0.07181	0.0708	0.73594	0.73628	0.5625	0.57023	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53316	53316	SRR9849219	SRX6603869	SRS5168918	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 3 mpf replicate 3	wt 14746 f kidney	strain:AB\|age:3months\|sex:male\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 3\|BioSampleModel:Model organism or animal	wildtype kidney 3 mpf replicate 3	wt 14746 f kidney	wt 14746 f kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-f-kidney_merged_S10-1.fastq.gz wt-14746-f-kidney_merged_S10-2.fastq.gz	fastq fastq	5697834151.0	38061527.0	wt 14746 f kidney merged S10 1.fastq.gz	0:74.86 1:74.84	A:1488491305;C:1328009157;G:1311260167;T:1524663985;N:45409537	74	74	1488491305	1328009157	1311260167	1524663985	45409537	SRX6603869	SRS5168918	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94873	0.9525	0.0943	0.09336	0.71709	0.71741	0.53907	0.55371	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53317	53317	SRR9849216	SRX6603868	SRS5168917	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 3 mpf replicate 4	wt 14746 h kidney	strain:AB\|age:3months\|sex:male\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 4\|BioSampleModel:Model organism or animal	wildtype kidney 3 mpf replicate 4	wt 14746 h kidney	wt 14746 h kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-h-kidney_merged_S34-1.fastq.gz wt-14746-h-kidney_merged_S34-2.fastq.gz	fastq fastq	4802949250.0	32003702.0	wt 14746 h kidney merged S34 1.fastq.gz	0:75.05 1:75.02	A:1247408978;C:1132423247;G:1119030906;T:1277389500;N:26696619	75	75	1247408978	1132423247	1119030906	1277389500	26696619	SRX6603868	SRS5168917	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94868	0.95207	0.1008	0.1002	0.70654	0.70583	0.543	0.53646	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53318	53318	SRR9849217	SRX6603867	SRS5168916	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 3 mpf replicate 1	wt 14746 c kidney	strain:AB\|age:3months\|sex:male\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 1\|BioSampleModel:Model organism or animal	wildtype kidney 3 mpf replicate 1	wt 14746 c kidney	wt 14746 c kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-c-kidney_merged_S69-1.fastq.gz wt-14746-c-kidney_merged_S69-2.fastq.gz	fastq fastq	2926155789.0	19616550.0	wt 14746 c kidney merged S69 1.fastq.gz	0:74.59 1:74.58	A:738508985;C:703442172;G:697795154;T:755346949;N:31062529	74	74	738508985	703442172	697795154	755346949	31062529	SRX6603867	SRS5168916	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95391	0.95737	0.08073	0.08025	0.72273	0.72265	0.50256	0.53781	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53319	53319	SRR9849218	SRX6603866	SRS5168915	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 3 mpf replicate 5	wt 14746 b kidney	strain:AB\|age:3months\|sex:female\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 5\|BioSampleModel:Model organism or animal	wildtype kidney 3 mpf replicate 5	wt 14746 b kidney	wt 14746 b kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-b-kidney_merged_S57-1.fastq.gz wt-14746-b-kidney_merged_S57-2.fastq.gz	fastq fastq	3580693879.0	24148496.0	wt 14746 b kidney merged S57 1.fastq.gz	0:74.13 1:74.15	A:906877863;C:847589932;G:842258575;T:927440495;N:56527014	74	74	906877863	847589932	842258575	927440495	56527014	SRX6603866	SRS5168915	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94558	0.95003	0.09575	0.09468	0.72099	0.72192	0.52455	0.51579	75	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53377	53377	SRR9849279	SRX6603808	SRS5168857	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 5 mpf replicate 7	wt 14560 f kidney	strain:AB\|age:5months\|sex:female\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 7\|BioSampleModel:Model organism or animal	wildtype kidney 5 mpf replicate 7	wt 14560 f kidney	wt 14560 f kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-f-kidney_merged_S65-1.fastq.gz wt-14560-f-kidney_merged_S65-2.fastq.gz	fastq fastq	3381175351.0	22760562.0	wt 14560 f kidney merged S65 1.fastq.gz	0:74.29 1:74.26	A:834174555;C:827862556;G:819581002;T:856481808;N:43075430	74	74	834174555	827862556	819581002	856481808	43075430	SRX6603808	SRS5168857	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.96026	0.96284	0.07018	0.06939	0.69662	0.69647	0.48686	0.48311	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
53378	53378	SRR9849280	SRX6603807	SRS5168856	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype kidney 5 mpf replicate 6	wt 14560 d kidney	strain:AB\|age:5months\|sex:female\|tissue:kidney\|genotype:wildtype\|Replicate:Bilogical replicate 6\|BioSampleModel:Model organism or animal	wildtype kidney 5 mpf replicate 6	wt 14560 d kidney	wt 14560 d kidney	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-d-kidney_merged_S41-1.fastq.gz wt-14560-d-kidney_merged_S41-2.fastq.gz	fastq fastq	3260080878.0	21943002.0	wt 14560 d kidney merged S41 1.fastq.gz	0:74.31 1:74.26	A:781693169;C:817346676;G:813482706;T:801873503;N:45684824	74	74	781693169	817346676	813482706	801873503	45684824	SRX6603807	SRS5168856	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.96382	0.96558	0.05582	0.05496	0.72245	0.72342	0.50059	0.50428	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Kidney	Renal System
62687	62687	SRR13319605	SRX9747096	SRS7934718	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	Mut 10d k1	Mut 10d k1	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:15 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 1\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	Mut 10d k1	Mut 10d k1	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	Mut_10d_k1.raw_1.fastq.gz Mut_10d_k1.raw_2.fastq.gz	fastq fastq	6906287100.0	23020957.0	Mut 10d k1.raw 1.fastq.gz	0:150 1:150	A:1908295029;C:1526919757;G:1627893432;T:1843041112;N:137770	150	150	1908295029	1526919757	1627893432	1843041112	137770	SRX9747096	SRS7934718	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.94038	0.93872	0.08297	0.08272	0.71382	0.71539	0.54124	0.54083	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62688	62688	SRR13319606	SRX9747095	SRS7934719	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	WT 10d k3	WT 10d k3	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:14 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 3\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	WT 10d k3	WT 10d k3	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	WT_10d_k3.raw_1.fastq.gz WT_10d_k3.raw_2.fastq.gz	fastq fastq	7159590600.0	23865302.0	WT 10d k3.raw 1.fastq.gz	0:150 1:150	A:1984034638;C:1574065265;G:1674336416;T:1927009990;N:144291	150	150	1984034638	1574065265	1674336416	1927009990	144291	SRX9747095	SRS7934719	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.92946	0.92803	0.08386	0.08381	0.71052	0.71074	0.51146	0.51825	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62689	62689	SRR13319607	SRX9747094	SRS7934714	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	WT 10d k2	WT 10d k2	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:13 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 2\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	WT 10d k2	WT 10d k2	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	WT_10d_k2.raw_1.fastq.gz WT_10d_k2.raw_2.fastq.gz	fastq fastq	6354585300.0	21181951.0	WT 10d k2.raw 1.fastq.gz	0:150 1:150	A:1749380892;C:1406921465;G:1504331323;T:1693821680;N:129940	150	150	1749380892	1406921465	1504331323	1693821680	129940	SRX9747094	SRS7934714	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.93216	0.9306	0.0766	0.07651	0.70847	0.70936	0.53878	0.53632	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62690	62690	SRR13319608	SRX9747093	SRS7934720	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	WT 10d k1	WT 10d k1	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:12 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 1\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	WT 10d k1	WT 10d k1	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	WT_10d_k1.raw_1.fastq.gz WT_10d_k1.raw_2.fastq.gz	fastq fastq	8712565800.0	29041886.0	WT 10d k1.raw 1.fastq.gz	0:150 1:150	A:2388117969;C:1934065753;G:2091791049;T:2298416148;N:174881	150	150	2388117969	1934065753	2091791049	2298416148	174881	SRX9747093	SRS7934720	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.93468	0.93398	0.09783	0.0975	0.69593	0.69649	0.52524	0.52599	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62691	62691	SRR13319609	SRX9747092	SRS7934712	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	Mut 6h k3	Mut 6h k3	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:11 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 3\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	Mut 6h k3	Mut 6h k3	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	Mut_6h_k3.raw_1.fastq.gz Mut_6h_k3.raw_2.fastq.gz	fastq fastq	6821167500.0	22737225.0	Mut 6h k3.raw 1.fastq.gz	0:150 1:150	A:1903976437;C:1488858303;G:1566082857;T:1862227563;N:22340	150	150	1903976437	1488858303	1566082857	1862227563	22340	SRX9747092	SRS7934712	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.93422	0.93209	0.09882	0.09902	0.71784	0.71892	0.53469	0.5464	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62692	62692	SRR13319610	SRX9747091	SRS7934717	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	Mut 6h k2	Mut 6h k2	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:10 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 2\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	Mut 6h k2	Mut 6h k2	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	Mut_6h_k2.raw_1.fastq.gz Mut_6h_k2.raw_2.fastq.gz	fastq fastq	6614007900.0	22046693.0	Mut 6h k2.raw 1.fastq.gz	0:150 1:150	A:1836116506;C:1454221417;G:1524536821;T:1799111318;N:21838	150	150	1836116506	1454221417	1524536821	1799111318	21838	SRX9747091	SRS7934717	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.93405	0.93165	0.09928	0.0998	0.71378	0.71405	0.53405	0.53636	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62693	62693	SRR13319611	SRX9747090	SRS7934716	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	Mut 6h k1	Mut 6h k1	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:9 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 1\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	Mut 6h k1	Mut 6h k1	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	Mut_6h_k1.raw_1.fastq.gz Mut_6h_k1.raw_2.fastq.gz	fastq fastq	6856096500.0	22853655.0	Mut 6h k1.raw 1.fastq.gz	0:150 1:150	A:1914687245;C:1494001561;G:1567738680;T:1879647930;N:21084	150	150	1914687245	1494001561	1567738680	1879647930	21084	SRX9747090	SRS7934716	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.92882	0.92611	0.10791	0.10832	0.71378	0.71427	0.53012	0.52707	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62694	62694	SRR13319612	SRX9747089	SRS7934713	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	WT 6h k3	WT 6h k3	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:8 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 3\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	WT 6h k3	WT 6h k3	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	WT_6h_k3.raw_1.fastq.gz WT_6h_k3.raw_2.fastq.gz	fastq fastq	7194856500.0	23982855.0	WT 6h k3.raw 1.fastq.gz	0:150 1:150	A:2000223263;C:1580258499;G:1657803759;T:1956549208;N:21771	150	150	2000223263	1580258499	1657803759	1956549208	21771	SRX9747089	SRS7934713	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.93193	0.92986	0.09623	0.09609	0.70907	0.71029	0.53843	0.53754	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62695	62695	SRR13319613	SRX9747088	SRS7934715	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	Mut 10d k3	Mut 10d k3	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:17 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 3\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	Mut 10d k3	Mut 10d k3	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	Mut_10d_k3.raw_1.fastq.gz Mut_10d_k3.raw_2.fastq.gz	fastq fastq	7859046900.0	26196823.0	Mut 10d k3.raw 1.fastq.gz	0:150 1:150	A:2162835249;C:1743162639;G:1857995098;T:2094896175;N:157739	150	150	2162835249	1743162639	1857995098	2094896175	157739	SRX9747088	SRS7934715	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.93831	0.93732	0.0836	0.08331	0.69988	0.70132	0.54542	0.54383	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62696	62696	SRR13319614	SRX9747087	SRS7934710	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	Mut 10d k2	Mut 10d k2	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:16 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 2\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	Mut 10d k2	Mut 10d k2	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	Mut_10d_k2.raw_1.fastq.gz Mut_10d_k2.raw_2.fastq.gz	fastq fastq	6163552500.0	20545175.0	Mut 10d k2.raw 1.fastq.gz	0:150 1:150	A:1690284188;C:1371212067;G:1471613432;T:1630318376;N:124437	150	150	1690284188	1371212067	1471613432	1630318376	124437	SRX9747087	SRS7934710	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.94142	0.93953	0.07637	0.07599	0.7123	0.71323	0.54883	0.55044	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62697	62697	SRR13319615	SRX9747086	SRS7934709	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	WT 6h k2	WT 6h k2	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:7 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 2\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	WT 6h k2	WT 6h k2	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	WT_6h_k2.raw_1.fastq.gz WT_6h_k2.raw_2.fastq.gz	fastq fastq	7675977600.0	25586592.0	WT 6h k2.raw 1.fastq.gz	0:150 1:150	A:2123159765;C:1695880551;G:1771133441;T:2085779945;N:23898	150	150	2123159765	1695880551	1771133441	2085779945	23898	SRX9747086	SRS7934709	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.93307	0.93101	0.09081	0.09046	0.7097	0.71031	0.52694	0.52787	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62698	62698	SRR13319616	SRX9747085	SRS7934711	SRP299583	PRJNA688352	Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	PRJNA688352	Other	A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells	WT 6h k1	WT 6h k1	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:6 month\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 1\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish post vaccination	WT 6h k1	WT 6h k1	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299583	WT_6h_k1.raw_1.fastq.gz WT_6h_k1.raw_2.fastq.gz	fastq fastq	5660758200.0	18869194.0	WT 6h k1.raw 1.fastq.gz	0:150 1:150	A:1560962707;C:1253335257;G:1319974501;T:1526464986;N:20749	150	150	1560962707	1253335257	1319974501	1526464986	20749	SRX9747085	SRS7934711	SRA1178602	southwest university\|college of fisheries	southwest university	2	0.93079	0.92974	0.09975	0.09988	0.70504	0.70581	0.53219	0.533	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-28	Adult	Adult	Kidney	Renal System
62700	62700	SRR13327930	SRX9755046	SRS7944756	SRP299775	PRJNA688655	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	PRJNA688655	Other		Mut 0h k3	Mut 0h k3	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:11\|dev stage:mission\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 3\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	Mut 0h k3	Mut k3	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299775	WT_0h_k3.raw_1.fastq.gz WT_0h_k3.raw_2.fastq.gz	fastq fastq	6675348000.0	22251160.0	WT 0h k3.raw 1.fastq.gz	0:150 1:150	A:1848988411;C:1470625677;G:1544465916;T:1811246237;N:21759	150	150	1848988411	1470625677	1544465916	1811246237	21759	SRX9755046	SRS7944756	SRA1178968	southwest university\|college of fisheries	southwest university	2	0.86263	0.92706	0.08978	0.0978	0.70502	0.69895	0.55273	0.55505	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-30	Undetermined	Undetermined	Kidney	Renal System
62701	62701	SRR13327931	SRX9755045	SRS7944755	SRP299775	PRJNA688655	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	PRJNA688655	Other		Mut 0h k2	Mut 0h k2	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:10\|dev stage:mission\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 2\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	Mut 0h k2	Mut k2	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299775	WT_0h_k2.raw_1.fastq.gz WT_0h_k2.raw_2.fastq.gz	fastq fastq	7165537800.0	23885126.0	WT 0h k2.raw 1.fastq.gz	0:150 1:150	A:1961366053;C:1599856726;G:1683336568;T:1920956264;N:22189	150	150	1961366053	1599856726	1683336568	1920956264	22189	SRX9755045	SRS7944755	SRA1178968	southwest university\|college of fisheries	southwest university	2	0.93437	0.93173	0.09166	0.09117	0.6997	0.70031	0.5364	0.53886	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-30	Undetermined	Undetermined	Kidney	Renal System
62702	62702	SRR13327932	SRX9755044	SRS7944754	SRP299775	PRJNA688655	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	PRJNA688655	Other		Mut 0h k1	Mut 0h k1	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:9\|dev stage:mission\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 1\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	Mut 0h k1	Mut k1	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299775	WT_0h_k1.raw_1.fastq.gz WT_0h_k1.raw_2.fastq.gz	fastq fastq	5953238400.0	19844128.0	WT 0h k1.raw 1.fastq.gz	0:150 1:150	A:1643432335;C:1323053555;G:1377906382;T:1608824934;N:21194	150	150	1643432335	1323053555	1377906382	1608824934	21194	SRX9755044	SRS7944754	SRA1178968	southwest university\|college of fisheries	southwest university	2	0.93408	0.93293	0.08519	0.08534	0.70952	0.71029	0.56062	0.52712	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-30	Undetermined	Undetermined	Kidney	Renal System
62703	62703	SRR13327933	SRX9755043	SRS7944753	SRP299775	PRJNA688655	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	PRJNA688655	Other		WT 0h k3	WT 0h k3	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:8\|dev stage:mission\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 3\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	WT 0h k3	WT k3	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299775	Mut_0h_k3.raw_1.fastq.gz Mut_0h_k3.raw_2.fastq.gz	fastq fastq	5891495100.0	19638317.0	Mut 0h k3.raw 1.fastq.gz	0:150 1:150	A:1626936306;C:1303639063;G:1370950045;T:1589948552;N:21134	150	150	1626936306	1303639063	1370950045	1589948552	21134	SRX9755043	SRS7944753	SRA1178968	southwest university\|college of fisheries	southwest university	2	0.93117	0.93006	0.09742	0.09724	0.70396	0.7041	0.53898	0.53936	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-30	Undetermined	Undetermined	Kidney	Renal System
62704	62704	SRR13327934	SRX9755042	SRS7944752	SRP299775	PRJNA688655	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	PRJNA688655	Other		WT 0h k2	WT 0h k2	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:7\|dev stage:mission\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 2\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	WT 0h k2	WT k2	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299775	Mut_0h_k2.raw_1.fastq.gz Mut_0h_k2.raw_2.fastq.gz	fastq fastq	5543211000.0	18477370.0	Mut 0h k2.raw 1.fastq.gz	0:150 1:150	A:1531314014;C:1225009325;G:1291427420;T:1495440150;N:20091	150	150	1531314014	1225009325	1291427420	1495440150	20091	SRX9755042	SRS7944752	SRA1178968	southwest university\|college of fisheries	southwest university	2	0.93219	0.93172	0.10466	0.10531	0.70141	0.70104	0.53257	0.53162	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-30	Undetermined	Undetermined	Kidney	Renal System
62705	62705	SRR13327935	SRX9755041	SRS7944751	SRP299775	PRJNA688655	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	PRJNA688655	Other		WT 0h k1	WT 0h k1	strain:AB\|isolate:missing\|breed:missing\|cultivar:missing\|ecotype:missing\|age:6\|dev stage:mission\|sex:not collected\|tissue:kidney and headkidney\|biomaterial provider:Southwest University China\|replicate:Biological replicate 1\|BioSampleModel:Model organism or animal	RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish	WT 0h k1	WT k1	other	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	HiSeq X Ten	SRP299775	Mut_0h_k1.raw_1.fastq.gz Mut_0h_k1.raw_2.fastq.gz	fastq fastq	6954804600.0	23182682.0	Mut 0h k1.raw 1.fastq.gz	0:150 1:150	A:1929335607;C:1531367791;G:1605966700;T:1888112325;N:22177	150	150	1929335607	1531367791	1605966700	1888112325	22177	SRX9755041	SRS7944751	SRA1178968	southwest university\|college of fisheries	southwest university	2	0.9401	0.93841	0.08725	0.08725	0.71536	0.71569	0.56381	0.56397	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	other	unknown	bulk	unknown	unknown	China	2020-12-30	Undetermined	Undetermined	Kidney	Renal System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;