metadata: run_metadata: 28 rows where experiment.library_selection = "other", experiment.library_strategy = "RNA-Seq" and tissue

28 rows where experiment.library_selection = "other", experiment.library_strategy = "RNA-Seq" and tissue_curation = "Gill"

descending

Link	rowid ▼	run.accession	experiment.accession	sample.accession	study.accession	bioproject	study.title	study.alias	study.type	study.abstract	sample.title	sample.alias	sample.attributes	experiment.title	experiment.alias	experiment.library_name	experiment.design_description	experiment.library_strategy	experiment.library_source	experiment.library_selection	experiment.library_layout	experiment.platform	experiment.instrument_model	experiment.study_ref	run.filename	run.semantic_name	run.total_bases	run.total_spots	run.alias	run.read_lengths	run.base_counts	run.r1_length	run.r2_length	run.Acount	run.Ccount	run.Gcount	run.Tcount	run.Ncount	run.experiment	run.pool_member	submission.accession	submission.srasource	submission.bioprojectsource	seqdetective.n_mates	seqdetective.mapping_rate.mate1	seqdetective.mapping_rate.mate2	seqdetective.nofeature_rate.mate1	seqdetective.nofeature_rate.mate2	seqdetective.sparsity.mate1	seqdetective.sparsity.mate2	seqdetective.pos_strand_rate.mate1	seqdetective.pos_strand_rate.mate2	seqdetective.readlen.mate1	seqdetective.readlen.mate2	seqdetective.judgement.mate1	seqdetective.judgement.mate2	seqdetective.judgement.reason	platform_family	instrument_generation	read_bias	selection_class	prep_kit	sc_or_bulk	tech_class	technology	submission.bioprojectsource.country	earliest_date	devstage_curation	devstage_curation_coarse	tissue_curation	tissue_curation_coarse
53349	53349	SRR9849251	SRX6603836	SRS5168885	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 3 mpf replicate 6	kl 14744 d gill	strain:AB\|age:3months\|sex:female\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 6\|BioSampleModel:Model organism or animal	Klotho gill 3 mpf replicate 6	kl 14744 d gill	kl 14744 d gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-d-gill_merged_S63-1.fastq.gz kl-14744-d-gill_merged_S63-2.fastq.gz	fastq fastq	3202652208.0	21426652.0	kl 14744 d gill merged S63 1.fastq.gz	0:74.76 1:74.71	A:787284556;C:792881443;G:786148640;T:806832731;N:29504838	74	74	787284556	792881443	786148640	806832731	29504838	SRX6603836	SRS5168885	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95184	0.95423	0.08279	0.08221	0.6925	0.69244	0.50117	0.49895	75	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53350	53350	SRR9849256	SRX6603835	SRS5168884	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 3 mpf replicate 7	kl 14744 f gill	strain:AB\|age:3months\|sex:female\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 7\|BioSampleModel:Model organism or animal	Klotho gill 3 mpf replicate 7	kl 14744 f gill	kl 14744 f gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-f-gill_merged_S28-1.fastq.gz kl-14744-f-gill_merged_S28-2.fastq.gz	fastq fastq	3290448619.0	22023416.0	kl 14744 f gill merged S28 1.fastq.gz	0:74.72 1:74.69	A:852051739;C:772110143;G:764138186;T:870831842;N:31316709	74	74	852051739	772110143	764138186	870831842	31316709	SRX6603835	SRS5168884	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93891	0.94218	0.11083	0.10988	0.68682	0.68732	0.51269	0.51959	76	74	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53351	53351	SRR9849252	SRX6603834	SRS5168883	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 3 mpf replicate 8	kl 14744 h gill	strain:AB\|age:3months\|sex:female\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 8\|BioSampleModel:Model organism or animal	Klotho gill 3 mpf replicate 8	kl 14744 h gill	kl 14744 h gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-h-gill_merged_S88-1.fastq.gz kl-14744-h-gill_merged_S88-2.fastq.gz	fastq fastq	2993191869.0	20020896.0	kl 14744 h gill merged S88 1.fastq.gz	0:74.77 1:74.73	A:745064408;C:729194088;G:729776159;T:761918449;N:27238765	74	74	745064408	729194088	729776159	761918449	27238765	SRX6603834	SRS5168883	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94847	0.95062	0.08456	0.08377	0.6942	0.69576	0.51192	0.51533	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53352	53352	SRR9849255	SRX6603833	SRS5168882	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 5 mpf replicate 1	kl 14562 a gill	strain:AB\|age:5months\|sex:male\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 1\|BioSampleModel:Model organism or animal	Klotho gill 5 mpf replicate 1	kl 14562 a gill	kl 14562 a gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14562-a-gill_merged_S62-1.fastq.gz kl-14562-a-gill_merged_S62-2.fastq.gz	fastq fastq	4948315549.0	33026008.0	kl 14562 a gill merged S62 1.fastq.gz	0:74.93 1:74.90	A:1264379777;C:1187097302;G:1173849990;T:1292353460;N:30635020	74	74	1264379777	1187097302	1173849990	1292353460	30635020	SRX6603833	SRS5168882	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94579	0.9481	0.10417	0.10358	0.69118	0.69179	0.49146	0.50019	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53353	53353	SRR9849253	SRX6603832	SRS5168881	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 5 mpf replicate 2	kl 14563 a gill	strain:AB\|age:5months\|sex:male\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 2\|BioSampleModel:Model organism or animal	Klotho gill 5 mpf replicate 2	kl 14563 a gill	kl 14563 a gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14563-a-gill_merged_S26-1.fastq.gz kl-14563-a-gill_merged_S26-2.fastq.gz	fastq fastq	4105985705.0	27689417.0	kl 14563 a gill merged S26 1.fastq.gz	0:74.15 1:74.14	A:1073116772;C:944775687;G:934585663;T:1094337784;N:59169799	74	74	1073116772	944775687	934585663	1094337784	59169799	SRX6603832	SRS5168881	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93325	0.93593	0.1444	0.14317	0.68858	0.68878	0.5035	0.50318	75	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53354	53354	SRR9849254	SRX6603831	SRS5168880	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 5 mpf replicate 3	kl 14563 d gill	strain:AB\|age:5months\|sex:male\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 3\|BioSampleModel:Model organism or animal	Klotho gill 5 mpf replicate 3	kl 14563 d gill	kl 14563 d gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14563-d-gill_merged_S73-1.fastq.gz kl-14563-d-gill_merged_S73-2.fastq.gz	fastq fastq	4961539887.0	40344254.0	kl 14563 d gill merged S73 1.fastq.gz	0:61.28 1:61.70	A:976358671;C:989029058;G:1003562439;T:1017674045;N:974915674	61	61	976358671	989029058	1003562439	1017674045	974915674	SRX6603831	SRS5168880	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94266	0.94526	0.08453	0.08322	0.73003	0.73154	0.52135	0.52198	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53355	53355	SRR9849257	SRX6603830	SRS5168879	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 5 mpf replicate 4	kl 14563 b gill	strain:AB\|age:5months\|sex:female\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 4\|BioSampleModel:Model organism or animal	Klotho gill 5 mpf replicate 4	kl 14563 b gill	kl 14563 b gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14563-b-gill_merged_S38-1.fastq.gz kl-14563-b-gill_merged_S38-2.fastq.gz	fastq fastq	4422729183.0	29816574.0	kl 14563 b gill merged S38 1.fastq.gz	0:74.17 1:74.16	A:1107565973;C:1062284875;G:1052483834;T:1134901393;N:65493108	74	74	1107565973	1062284875	1052483834	1134901393	65493108	SRX6603830	SRS5168879	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94675	0.94841	0.11178	0.11063	0.68438	0.68619	0.50291	0.50492	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53356	53356	SRR9849258	SRX6603829	SRS5168878	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 5 mpf replicate 5	kl 14563 c gill	strain:AB\|age:5months\|sex:female\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 5\|BioSampleModel:Model organism or animal	Klotho gill 5 mpf replicate 5	kl 14563 c gill	kl 14563 c gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14563-c-gill_merged_S49-1.fastq.gz kl-14563-c-gill_merged_S49-2.fastq.gz	fastq fastq	3023771958.0	21203385.0	kl 14563 c gill merged S49 1.fastq.gz	0:71.29 1:71.32	A:724268289;C:701710927;G:696152349;T:747721141;N:153919252	71	71	724268289	701710927	696152349	747721141	153919252	SRX6603829	SRS5168878	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95184	0.95462	0.09513	0.09444	0.70386	0.70506	0.52039	0.52251	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53357	53357	SRR9849259	SRX6603828	SRS5168877	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 3 mpf replicate 1	kl 14744 a gill	strain:AB\|age:3months\|sex:male\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 1\|BioSampleModel:Model organism or animal	Klotho gill 3 mpf replicate 1	kl 14744 a gill	kl 14744 a gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-a-gill_merged_S3-1.fastq.gz kl-14744-a-gill_merged_S3-2.fastq.gz	fastq fastq	4472258246.0	30417040.0	kl 14744 a gill merged S3 1.fastq.gz	0:73.52 1:73.52	A:1163792021;C:1015967093;G:1005569946;T:1189321143;N:97608043	73	73	1163792021	1015967093	1005569946	1189321143	97608043	SRX6603828	SRS5168877	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93243	0.93488	0.11957	0.11821	0.69043	0.68982	0.4947	0.50103	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53358	53358	SRR9849260	SRX6603827	SRS5168876	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 5 mpf replicate 7	wt 14560 h gill	strain:AB\|age:5months\|sex:female\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 7\|BioSampleModel:Model organism or animal	wildtype gill 5 mpf replicate 7	wt 14560 h gill	wt 14560 h gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-h-gill_merged_S86-1.fastq.gz wt-14560-h-gill_merged_S86-2.fastq.gz	fastq fastq	3856374025.0	26027532.0	wt 14560 h gill merged S86 1.fastq.gz	0:74.10 1:74.07	A:904187535;C:977357747;G:982381061;T:926724178;N:65723504	74	74	904187535	977357747	982381061	926724178	65723504	SRX6603827	SRS5168876	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95669	0.95945	0.08449	0.08231	0.70378	0.70662	0.51566	0.51794	75	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53359	53359	SRR9849261	SRX6603826	SRS5168875	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 3 mpf replicate 3	kl 14744 e gill	strain:AB\|age:3months\|sex:male\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 3\|BioSampleModel:Model organism or animal	Klotho gill 3 mpf replicate 3	kl 14744 e gill	kl 14744 e gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-e-gill_merged_S4-1.fastq.gz kl-14744-e-gill_merged_S4-2.fastq.gz	fastq fastq	5367136114.0	36130715.0	kl 14744 e gill merged S4 1.fastq.gz	0:74.28 1:74.27	A:1423114942;C:1217135684;G:1200462354;T:1454946366;N:71476768	74	74	1423114942	1217135684	1200462354	1454946366	71476768	SRX6603826	SRS5168875	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.92473	0.92706	0.14361	0.14221	0.68314	0.68215	0.49619	0.49814	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53360	53360	SRR9849262	SRX6603825	SRS5168874	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 3 mpf replicate 2	kl 14744 c gill	strain:AB\|age:3months\|sex:male\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 2\|BioSampleModel:Model organism or animal	Klotho gill 3 mpf replicate 2	kl 14744 c gill	kl 14744 c gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-c-gill_merged_S51-1.fastq.gz kl-14744-c-gill_merged_S51-2.fastq.gz	fastq fastq	3781913771.0	25535680.0	kl 14744 c gill merged S51 1.fastq.gz	0:74.05 1:74.06	A:973851133;C:877578711;G:873528708;T:994449171;N:62506048	74	74	973851133	877578711	873528708	994449171	62506048	SRX6603825	SRS5168874	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93396	0.93615	0.11943	0.11847	0.69428	0.69597	0.51454	0.51378	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53361	53361	SRR9849263	SRX6603824	SRS5168873	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 5 mpf replicate 4	wt 14560 g gill	strain:AB\|age:5months\|sex:male\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 4\|BioSampleModel:Model organism or animal	wildtype gill 5 mpf replicate 4	wt 14560 g gill	wt 14560 g gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-g-gill_merged_S74-1.fastq.gz wt-14560-g-gill_merged_S74-2.fastq.gz	fastq fastq	3197383391.0	23146133.0	wt 14560 g gill merged S74 1.fastq.gz	0:68.96 1:69.18	A:761238423;C:704040317;G:699107362;T:780204404;N:252792885	68	69	761238423	704040317	699107362	780204404	252792885	SRX6603824	SRS5168873	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.9322	0.93439	0.11465	0.11387	0.6996	0.70145	0.51674	0.52391	59	59	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53362	53362	SRR9849264	SRX6603823	SRS5168872	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 5 mpf replicate 3	wt 14560 e gill	strain:AB\|age:5months\|sex:male\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 3\|BioSampleModel:Model organism or animal	wildtype gill 5 mpf replicate 3	wt 14560 e gill	wt 14560 e gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-e-gill_merged_S37-1.fastq.gz wt-14560-e-gill_merged_S37-2.fastq.gz	fastq fastq	6534462524.0	46930991.0	wt 14560 e gill merged S37 1.fastq.gz	0:69.60 1:69.64	A:1419832692;C:1577789914;G:1592333860;T:1463766163;N:480739895	69	69	1419832692	1577789914	1592333860	1463766163	480739895	SRX6603823	SRS5168872	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95759	0.96007	0.08549	0.08527	0.72352	0.7263	0.51737	0.51106	76	71	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53363	53363	SRR9849265	SRX6603822	SRS5168871	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 5 mpf replicate 6	wt 14560 f gill	strain:AB\|age:5months\|sex:female\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 6\|BioSampleModel:Model organism or animal	wildtype gill 5 mpf replicate 6	wt 14560 f gill	wt 14560 f gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-f-gill_merged_S61-1.fastq.gz wt-14560-f-gill_merged_S61-2.fastq.gz	fastq fastq	8181016280.0	56620046.0	wt 14560 f gill merged S61 1.fastq.gz	0:72.23 1:72.26	A:1923562561;C:1974860714;G:1970177126;T:1991223564;N:321192315	72	72	1923562561	1974860714	1970177126	1991223564	321192315	SRX6603822	SRS5168871	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95764	0.95938	0.08178	0.08069	0.69483	0.6955	0.4956	0.49531	75	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53364	53364	SRR9849266	SRX6603821	SRS5168870	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 5 mpf replicate 5	wt 14560 b gill	strain:AB\|age:5months\|sex:female\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 5\|BioSampleModel:Model organism or animal	wildtype gill 5 mpf replicate 5	wt 14560 b gill	wt 14560 b gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-b-gill_merged_S14-1.fastq.gz wt-14560-b-gill_merged_S14-2.fastq.gz	fastq fastq	2362372367.0	23358970.0	wt 14560 b gill merged S14 1.fastq.gz	0:49.78 1:51.36	A:342145885;C:327654164;G:338531990;T:348076960;N:1005963368	49	51	342145885	327654164	338531990	348076960	1005963368	SRX6603821	SRS5168870	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95925	0.96332	0.40158	0.40128	0.83307	0.83439	0.53786	0.53636	35	35	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53365	53365	SRR9849267	SRX6603820	SRS5168869	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 3 mpf replicate 5	kl 14744 b gill	strain:AB\|age:3months\|sex:female\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 5\|BioSampleModel:Model organism or animal	Klotho gill 3 mpf replicate 5	kl 14744 b gill	kl 14744 b gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-b-gill_merged_S15-1.fastq.gz kl-14744-b-gill_merged_S15-2.fastq.gz	fastq fastq	3896384369.0	26369685.0	kl 14744 b gill merged S15 1.fastq.gz	0:73.87 1:73.89	A:1006926925;C:896442422;G:893773611;T:1027571320;N:71670091	73	73	1006926925	896442422	893773611	1027571320	71670091	SRX6603820	SRS5168869	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93425	0.93671	0.10573	0.10362	0.68925	0.69055	0.50881	0.50958	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53366	53366	SRR9849268	SRX6603819	SRS5168868	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	αKlotho gill 3 mpf replicate 4	kl 14744 g gill	strain:AB\|age:3months\|sex:male\|tissue:gills\|genotype:aKlotho\|Replicate:Bilogical replicate 4\|BioSampleModel:Model organism or animal	Klotho gill 3 mpf replicate 4	kl 14744 g gill	kl 14744 g gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	kl-14744-g-gill_merged_S40-1.fastq.gz kl-14744-g-gill_merged_S40-2.fastq.gz	fastq fastq	4106174367.0	27588154.0	kl 14744 g gill merged S40 1.fastq.gz	0:74.44 1:74.40	A:1027552543;C:990923882;G:985638754;T:1052538016;N:49521172	74	74	1027552543	990923882	985638754	1052538016	49521172	SRX6603819	SRS5168868	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94712	0.94847	0.086	0.08492	0.69065	0.69213	0.50545	0.50658	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53367	53367	SRR9849269	SRX6603818	SRS5168867	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 5 mpf replicate 2	wt 14560 c gill	strain:AB\|age:5months\|sex:male\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 2\|BioSampleModel:Model organism or animal	wildtype gill 5 mpf replicate 2	wt 14560 c gill	wt 14560 c gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-c-gill_merged_S50-1.fastq.gz wt-14560-c-gill_merged_S50-2.fastq.gz	fastq fastq	3502888551.0	23649590.0	wt 14560 c gill merged S50 1.fastq.gz	0:74.05 1:74.07	A:903995121;C:812069624;G:808206962;T:922560926;N:56055918	74	74	903995121	812069624	808206962	922560926	56055918	SRX6603818	SRS5168867	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93602	0.93796	0.1196	0.11851	0.7054	0.70642	0.51487	0.51576	76	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53368	53368	SRR9849270	SRX6603817	SRS5168866	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 5 mpf replicate 1	wt 14560 a gill	strain:AB\|age:5months\|sex:male\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 1\|BioSampleModel:Model organism or animal	wildtype gill 5 mpf replicate 1	wt 14560 a gill	wt 14560 a gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14560-a-gill_merged_S2-1.fastq.gz wt-14560-a-gill_merged_S2-2.fastq.gz	fastq fastq	3961292962.0	26763233.0	wt 14560 a gill merged S2 1.fastq.gz	0:74.00 1:74.01	A:1030164139;C:909271583;G:896656858;T:1054638293;N:70562089	74	74	1030164139	909271583	896656858	1054638293	70562089	SRX6603817	SRS5168866	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93066	0.93386	0.11894	0.11767	0.70333	0.70305	0.52591	0.5296	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53369	53369	SRR9849271	SRX6603816	SRS5168865	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 3 mpf replicate 8	wt 14746 i gill	strain:AB\|age:3months\|sex:female\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 8\|BioSampleModel:Model organism or animal	wildtype gill 3 mpf replicate 8	wt 14746 i gill	wt 14746 i gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-i-gill_merged_S76-1.fastq.gz wt-14746-i-gill_merged_S76-2.fastq.gz	fastq fastq	3270182743.0	22131464.0	wt 14746 i gill merged S76 1.fastq.gz	0:73.88 1:73.89	A:804863458;C:789181461;G:789200500;T:825128130;N:61809194	73	73	804863458	789181461	789200500	825128130	61809194	SRX6603816	SRS5168865	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94667	0.94869	0.09194	0.09083	0.68982	0.69071	0.49863	0.49843	73	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53370	53370	SRR9849272	SRX6603815	SRS5168864	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 3 mpf replicate 7	wt 14746 g gill	strain:AB\|age:3months\|sex:female\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 7\|BioSampleModel:Model organism or animal	wildtype gill 3 mpf replicate 7	wt 14746 g gill	wt 14746 g gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-g-gill_merged_S52-1.fastq.gz wt-14746-g-gill_merged_S52-2.fastq.gz	fastq fastq	3993806223.0	26757111.0	wt 14746 g gill merged S52 1.fastq.gz	0:74.64 1:74.63	A:1042379582;C:926777409;G:917134457;T:1066753507;N:40761268	74	74	1042379582	926777409	917134457	1066753507	40761268	SRX6603815	SRS5168864	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.92989	0.93168	0.12566	0.12387	0.69483	0.69398	0.51742	0.51246	75	75	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53371	53371	SRR9849273	SRX6603814	SRS5168863	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 3 mpf replicate 6	wt 14746 d gill	strain:AB\|age:3months\|sex:female\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 6\|BioSampleModel:Model organism or animal	wildtype gill 3 mpf replicate 6	wt 14746 d gill	wt 14746 d gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-d-gill_merged_S75-1.fastq.gz wt-14746-d-gill_merged_S75-2.fastq.gz	fastq fastq	3446469484.0	23064072.0	wt 14746 d gill merged S75 1.fastq.gz	0:74.71 1:74.72	A:914383238;C:784073061;G:782840866;T:932786258;N:32386061	74	74	914383238	784073061	782840866	932786258	32386061	SRX6603814	SRS5168863	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93218	0.9355	0.13837	0.13689	0.69187	0.69449	0.50233	0.50335	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53372	53372	SRR9849274	SRX6603813	SRS5168862	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 3 mpf replicate 5	wt 14746 b gill	strain:AB\|age:3months\|sex:female\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 5\|BioSampleModel:Model organism or animal	wildtype gill 3 mpf replicate 5	wt 14746 b gill	wt 14746 b gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-b-gill_merged_S27-1.fastq.gz wt-14746-b-gill_merged_S27-2.fastq.gz	fastq fastq	2683814716.0	17955287.0	wt 14746 b gill merged S27 1.fastq.gz	0:74.75 1:74.73	A:709454000;C:617240570;G:609841284;T:722539050;N:24739812	74	74	709454000	617240570	609841284	722539050	24739812	SRX6603813	SRS5168862	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93811	0.94116	0.11249	0.11071	0.71386	0.71356	0.52649	0.5305	73	73	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53373	53373	SRR9849275	SRX6603812	SRS5168861	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 3 mpf replicate 4	wt 14746 h gill	strain:AB\|age:3months\|sex:male\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 4\|BioSampleModel:Model organism or animal	wildtype gill 3 mpf replicate 4	wt 14746 h gill	wt 14746 h gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-h-gill_merged_S64-1.fastq.gz wt-14746-h-gill_merged_S64-2.fastq.gz	fastq fastq	3456397754.0	23102971.0	wt 14746 h gill merged S64 1.fastq.gz	0:74.83 1:74.78	A:859599641;C:847277874;G:840222901;T:880593110;N:28704228	74	74	859599641	847277874	840222901	880593110	28704228	SRX6603812	SRS5168861	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.94758	0.9497	0.07851	0.07813	0.69213	0.69292	0.49624	0.49526	76	74	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53374	53374	SRR9849276	SRX6603811	SRS5168860	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 3 mpf replicate 3	wt 14746 f gill	strain:AB\|age:3months\|sex:male\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 3\|BioSampleModel:Model organism or animal	wildtype gill 3 mpf replicate 3	wt 14746 f gill	wt 14746 f gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-f-gill_merged_S16-1.fastq.gz wt-14746-f-gill_merged_S16-2.fastq.gz	fastq fastq	3246895297.0	22327407.0	wt 14746 f gill merged S16 1.fastq.gz	0:72.72 1:72.70	A:790813773;C:771278393;G:771142617;T:806255420;N:107405094	72	72	790813773	771278393	771142617	806255420	107405094	SRX6603811	SRS5168860	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.93991	0.94246	0.09684	0.09535	0.69613	0.69662	0.51599	0.51405	35	35	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53375	53375	SRR9849277	SRX6603810	SRS5168859	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 3 mpf replicate 2	wt 14746 e gill	strain:AB\|age:3months\|sex:male\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 2\|BioSampleModel:Model organism or animal	wildtype gill 3 mpf replicate 2	wt 14746 e gill	wt 14746 e gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-e-gill_merged_S87-1.fastq.gz wt-14746-e-gill_merged_S87-2.fastq.gz	fastq fastq	3327810713.0	22620977.0	wt 14746 e gill merged S87 1.fastq.gz	0:73.57 1:73.54	A:790747041;C:826908991;G:822421934;T:811444797;N:76287950	73	73	790747041	826908991	822421934	811444797	76287950	SRX6603810	SRS5168859	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.95403	0.95572	0.0703	0.07016	0.70291	0.70443	0.49483	0.50182	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System
53376	53376	SRR9849278	SRX6603809	SRS5168858	SRP216556	PRJNA556842	aKlotho regulates age associated vascular calcification and lifespan in zebrafish	PRJNA556842	Transcriptome Analysis	The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast its overexpression increases lifespan. Given the severe mouse phenotype we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body most dramatically in the outflow tract of the heart the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.	wildtype gill 3 mpf replicate 1	wt 14746 c gill	strain:AB\|age:3months\|sex:male\|tissue:gills\|genotype:wildtype\|Replicate:Bilogical replicate 1\|BioSampleModel:Model organism or animal	wildtype gill 3 mpf replicate 1	wt 14746 c gill	wt 14746 c gill	Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills heart and kidney were collected from 32 individual fish at two time points namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point 4 males 4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C and samples were stored at 80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing mercaptoethanol and stored at 80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.	RNA-Seq	TRANSCRIPTOMIC	other	PAIRED	ILLUMINA	Illumina HiSeq 2500	SRP216556	wt-14746-c-gill_merged_S39-1.fastq.gz wt-14746-c-gill_merged_S39-2.fastq.gz	fastq fastq	3137718864.0	21347890.0	wt 14746 c gill merged S39 1.fastq.gz	0:73.51 1:73.47	A:739814481;C:782304288;G:781747265;T:759470557;N:74382273	73	73	739814481	782304288	781747265	759470557	74382273	SRX6603809	SRS5168858	SRA927891	Novartis Institutes for Biomedical Research\|Chemical Biology and Therapeutics	Novartis Institutes for Biomedical Research	2	0.955	0.95885	0.07154	0.07213	0.71001	0.71163	0.50235	0.49593	76	76	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown	Germany	2019-07-27	Adult	Adult	Gill	Respiratory System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;