run_metadata
10 rows where experiment.library_selection = "other", experiment.library_source = "TRANSCRIPTOMIC" and tissue_curation_coarse = "Undetermined"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 33123 | 33123 | SRR29672615 | SRX25176099 | SRS21866000 | SRP517393 | PRJNA1130538 | ac4C transcriptomes of Zebrafish and Worm | GSE271258 | Other | ac4C modification appears in mutilple model organisms including Zebrafish and Worm Overall design: To investigate whether ac4C modification is involved in evolution we performed ac4C RIP seq on Zebrafish and Worm. | Zebrafish ac4C | GSM8372406 | tissue:Animal organ cells|cell type:Animal organ cells|genotype:Wild type|rip antibody:anti ac4C Abcam catalog No. ab252215|geo loc name:missing|collection date:missing | Zebrafish ac4C | The raw ac4C RIP seq data were aligned to genome reference sequences by Hisat2. The aligned reads were used for ac4C modification peak calling and the significant methylation was identified by exomepeak2 and the ac4C peak calling can be visualized by IGV software. The MetaTX was used to examine the distribution pattern of epitranscriptome profiles. The STREME was used to determine if the ac4C peaks contained the consensus of ac4C motif sequences. For mRNA seq the mRNA expression level was analyzed by StringTie and differentially expressed mRNAs were calculated by DEseq. The substrates of ac4C regulators were obtained from starBase v2.0. The statistical enrichment analysis of Gene Ontology GO and Kyoto Encyclopedia of Genes and Genomes KEGG pathway for differentially expressed genes DEGs and differentially methylated mRNAs were applied by DAVID. Assembly: danRer10 or WBcel235 Supplementary files format and content: The processed data files is in CSV format containing the expression levels and ac4C status changes for each gene. | Animal organ cells | Total RNA was extracted according to manufacturer’s instruction. The stranded RNA sequencing library was constructed by KC DigitalTM Stranded mRNA Library Prep Kit for Illumina® Catalog NO. DR08502 Wuhan Seqhealth Co. Ltd. China following the manufacturer’s instruction. The kit eliminates duplication bias in PCR and sequencing steps by using unique molecular identifier UMI of 8 random bases to label the pre amplified cDNA molecules. The library products corresponding to 200 500 bps were enriched | cell type:Animal organ cells|genotype:Wild type|rip antibody:anti ac4C Abcam catalog No. ab252215 | GSM8372406 | GSM8372406: Zebrafish ac4C; Danio rerio; RIP Seq | GSM8372406 r1 | GSM8372406 | 1 | Total RNA was extracted according to manufacturer's instruction. The stranded RNA sequencing library was constructed by KC DigitalTM Stranded mRNA Library Prep Kit for Illumina® Catalog NO. DR08502 Wuhan Seqhealth Co. Ltd. China following the manufacturer's instruction. The kit eliminates duplication bias in PCR and sequencing steps by using unique molecular identifier UMI of 8 random bases to label the pre amplified cDNA molecules. The library products corresponding to 200 500 bps were enriched | RIP-Seq | TRANSCRIPTOMIC | other | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP517393 | Fish_IP.clean.R2.fastq.gz Fish_IP.clean.R1.fastq.gz | fastq fastq | 816231517.0 | 3830403.0 | GSM8372406 r1 | 0:102.34 1:110.76 | A:199381841;C:207571478;G:205740791;T:203535549;N:1858 | 102 | 110 | 199381841 | 207571478 | 205740791 | 203535549 | 1858 | SRX25176099 | SRS21866000 | SRA1914369 | Fujian Medical University | Fujian Medical University | 2 | 0.60415 | 0.60545 | 0.08824 | 0.08794 | 0.78796 | 0.78733 | 0.43432 | 0.43842 | 126 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-07-01 | Undetermined | Undetermined | Undetermined | Undetermined | |||||||||||||
| 52210 | 52210 | SRR9021058 | SRX5799154 | SRS4730324 | SRP195685 | PRJNA541367 | Global transcriptomic analysis of zebrafish glucagon receptor mutant | PRJNA541367 | Other | We performed RNA sequencing RNA seq analysis of whole fish to provide a comprehensive view of its global transcriptomic regulation in this study. | WT 1 | replicate:biological replicate 1|strain:AB|age:7 days|sex:not applicable|tissue:total|BioSampleModel:Model organism or animal | Diano rerio transcriptome | WT 1 20190506 1 | WT 1 20190506 1 | RNA seq of WT Diano rerio | RNA-Seq | TRANSCRIPTOMIC | other | SINGLE | BGISEQ | BGISEQ-500 | SRP195685 | loader:fastq load.py | WT1.1.fq | fastq | 1094498950.0 | 21889979.0 | WT1.1.fq | 0:50 | A:291195764;C:255539280;G:262638316;T:284737693;N:387897 | 50 | 291195764 | 255539280 | 262638316 | 284737693 | 387897 | SRX5799154 | SRS4730324 | SRA883435 | Xiamen University|School of Pharmaceutical Sciences | Xiamen University | 1 | 0.94195 | 0.09713 | 0.66689 | 0.47749 | 50 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-18 | Larval | Larval | Undetermined | Undetermined | |||||||||||||||||||||||||||
| 52211 | 52211 | SRR9021059 | SRX5799153 | SRS4730323 | SRP195685 | PRJNA541367 | Global transcriptomic analysis of zebrafish glucagon receptor mutant | PRJNA541367 | Other | We performed RNA sequencing RNA seq analysis of whole fish to provide a comprehensive view of its global transcriptomic regulation in this study. | WT 2 | replicate:biological replicate 2|strain:AB|age:7 days|sex:not applicable|tissue:total|BioSampleModel:Model organism or animal | Diano rerio transcriptome | WT 2 20190506 2 | WT 2 20190506 2 | RNA seq of WT Diano rerio | RNA-Seq | TRANSCRIPTOMIC | other | SINGLE | BGISEQ | BGISEQ-500 | SRP195685 | loader:fastq load.py | WT2.1.fq | fastq | 1095133200.0 | 21902664.0 | WT2.1.fq | 0:50 | A:290594757;C:255644044;G:259681140;T:288014102;N:1199157 | 50 | 290594757 | 255644044 | 259681140 | 288014102 | 1199157 | SRX5799153 | SRS4730323 | SRA883435 | Xiamen University|School of Pharmaceutical Sciences | Xiamen University | 1 | 0.93368 | 0.09166 | 0.67085 | 0.46523 | 50 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-18 | Larval | Larval | Undetermined | Undetermined | |||||||||||||||||||||||||||
| 52212 | 52212 | SRR9021060 | SRX5799152 | SRS4730322 | SRP195685 | PRJNA541367 | Global transcriptomic analysis of zebrafish glucagon receptor mutant | PRJNA541367 | Other | We performed RNA sequencing RNA seq analysis of whole fish to provide a comprehensive view of its global transcriptomic regulation in this study. | WT 3 | replicate:biological replicate 3|strain:AB|age:7 days|sex:not applicable|tissue:total|BioSampleModel:Model organism or animal | Diano rerio transcriptome | WT 3 20190506 3 | WT 3 20190506 3 | RNA seq of WT Diano rerio | RNA-Seq | TRANSCRIPTOMIC | other | SINGLE | BGISEQ | BGISEQ-500 | SRP195685 | loader:fastq load.py | WT3.1.fq | fastq | 1096083900.0 | 21921678.0 | WT3.1.fq | 0:50 | A:290332094;C:256993500;G:263043956;T:285349722;N:364628 | 50 | 290332094 | 256993500 | 263043956 | 285349722 | 364628 | SRX5799152 | SRS4730322 | SRA883435 | Xiamen University|School of Pharmaceutical Sciences | Xiamen University | 1 | 0.94147 | 0.10157 | 0.66135 | 0.47301 | 50 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-18 | Larval | Larval | Undetermined | Undetermined | |||||||||||||||||||||||||||
| 52213 | 52213 | SRR9021061 | SRX5799151 | SRS4730320 | SRP195685 | PRJNA541367 | Global transcriptomic analysis of zebrafish glucagon receptor mutant | PRJNA541367 | Other | We performed RNA sequencing RNA seq analysis of whole fish to provide a comprehensive view of its global transcriptomic regulation in this study. | gcgr 1 | replicate:biological replicate 1|strain:AB|age:7 days|sex:not applicable|tissue:total|BioSampleModel:Model organism or animal | Diano rerio transcriptome | gcgr 1 20190506 1 | gcgr 1 20190506 1 | RNA seq of Diano rerio with gcgr mutant | RNA-Seq | TRANSCRIPTOMIC | other | SINGLE | BGISEQ | BGISEQ-500 | SRP195685 | loader:fastq load.py | gcgr1.1.fq | fastq | 1096307200.0 | 21926144.0 | gcgr1.1.fq | 0:50 | A:288839446;C:258297625;G:264630675;T:284199738;N:339716 | 50 | 288839446 | 258297625 | 264630675 | 284199738 | 339716 | SRX5799151 | SRS4730320 | SRA883435 | Xiamen University|School of Pharmaceutical Sciences | Xiamen University | 1 | 0.94131 | 0.09307 | 0.67574 | 0.46993 | 50 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-18 | Larval | Larval | Undetermined | Undetermined | |||||||||||||||||||||||||||
| 52214 | 52214 | SRR9021062 | SRX5799150 | SRS4730321 | SRP195685 | PRJNA541367 | Global transcriptomic analysis of zebrafish glucagon receptor mutant | PRJNA541367 | Other | We performed RNA sequencing RNA seq analysis of whole fish to provide a comprehensive view of its global transcriptomic regulation in this study. | gcgr 2 | replicate:biological replicate 2|strain:AB|age:7 days|sex:not applicable|tissue:total|BioSampleModel:Model organism or animal | Diano rerio transcriptome | gcgr 2 20190506 2 | gcgr 2 20190506 2 | RNA seq of Diano rerio with gcgr mutant | RNA-Seq | TRANSCRIPTOMIC | other | SINGLE | BGISEQ | BGISEQ-500 | SRP195685 | loader:fastq load.py | gcgr2.1.fq | fastq | 1094410100.0 | 21888202.0 | gcgr2.1.fq | 0:50 | A:290062779;C:256358687;G:264488431;T:282812212;N:687991 | 50 | 290062779 | 256358687 | 264488431 | 282812212 | 687991 | SRX5799150 | SRS4730321 | SRA883435 | Xiamen University|School of Pharmaceutical Sciences | Xiamen University | 1 | 0.94074 | 0.09337 | 0.67633 | 0.46861 | 50 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-18 | Larval | Larval | Undetermined | Undetermined | |||||||||||||||||||||||||||
| 52215 | 52215 | SRR9021063 | SRX5799149 | SRS4730319 | SRP195685 | PRJNA541367 | Global transcriptomic analysis of zebrafish glucagon receptor mutant | PRJNA541367 | Other | We performed RNA sequencing RNA seq analysis of whole fish to provide a comprehensive view of its global transcriptomic regulation in this study. | gcgr 3 | replicate:biological replicate 3|strain:AB|age:7 days|sex:not applicable|tissue:total|BioSampleModel:Model organism or animal | Diano rerio transcriptome | gcgr 3 20190506 3 | gcgr 3 20190506 3 | RNA seq of Diano rerio with gcgr mutant | RNA-Seq | TRANSCRIPTOMIC | other | SINGLE | BGISEQ | BGISEQ-500 | SRP195685 | loader:fastq load.py | gcgr3.1.fq | fastq | 1094284800.0 | 21885696.0 | gcgr3.1.fq | 0:50 | A:289313514;C:257096636;G:264011408;T:283143600;N:719642 | 50 | 289313514 | 257096636 | 264011408 | 283143600 | 719642 | SRX5799149 | SRS4730319 | SRA883435 | Xiamen University|School of Pharmaceutical Sciences | Xiamen University | 1 | 0.94222 | 0.09557 | 0.67521 | 0.46664 | 50 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-18 | Larval | Larval | Undetermined | Undetermined | |||||||||||||||||||||||||||
| 59503 | 59503 | SRR11924320 | SRX8469993 | SRS6770644 | SRP265951 | PRJNA637293 | The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2' O Me sites | GSE151797 | Other | A sequencing based profiling method RiboMeth seq for ribose methylations was used to study methylation patterns during Zebrafish Danio rerio development Overall design: All samples were analyzed in biological triplicates except for adult tail trunk that was in duplicate. | pubmed:32912962 | PM 3 | GSM4591060 | source name:protruding mouth stage|tissue:protruding mouth stage|rna fraction:size fractionated 20 40 nt whole cell RNA | PM 3 | Library strategy: RiboMeth seq Barcode separation using python script Adaptor trimming using Cutadapt v. 2.0 Mapping to rRNA reference sequence using Bowtie2 v. 2.3.4.1 Counting read ends and calculating RiboMeth seq scores using python scripts The output FASTA files from small RNA seq were merged and used as the basis of the SNORD search and rRNA interaction prediction. Initially SNORDs were identified by running the merged FASTA file through snoScan Schattner et al. 2005 against zebrafish early and late rRNA reference sequences Locati et al. 2017. Genome build: early and late zebrafish rRNA locati et al. The reference sequences are available in the FASTA file on the series record. Supplementary files format and content: MS Excel file contains five prime and three prime read count and calculated RiboMeth seq score at all positions in the rRNA sequence. | protruding mouth stage | Tissues were homogenized and whole cell RNA was extracted using Qiazol Qiagen according to the manufacturer. RiboMeth seq: 5 10 ug of RNA was partially degraded by alkaline at denaturing temperatures. The size fraction 20 40 nt was purified on gels and linkers added using a system relying on a modified Arabidopsis tRNA ligase joining 2' three prime cyclic phosphate and five prime phosphate ends. The library fragments were then sequenced on the Ion Proton platform. See Birkedal U Christensen Dalsgaard M Krogh N Sabarinathan R Gorodkin J Nielsen H. Profiling of ribose methylations in RNA by high throughput sequencing. Angewandte Chemie. 2015;542:451 5 for detailed description | tissue:protruding mouth stage|rna fraction:size fractionated 20 40 nt whole cell RNA | GSM4591060 | GSM4591060: PM 3; Danio rerio; OTHER | GSM4591060 | 1 | Tissues were homogenized and whole cell RNA was extracted using Qiazol Qiagen according to the manufacturer. RiboMeth seq: 5 10 ug of RNA was partially degraded by alkaline at denaturing temperatures. The size fraction 20 40 nt was purified on gels and linkers added using a system relying on a modified Arabidopsis tRNA ligase joining 2' three prime cyclic phosphate and five prime phosphate ends. The library fragments were then sequenced on the Ion Proton platform. See Birkedal U Christensen Dalsgaard M Krogh N Sabarinathan R Gorodkin J Nielsen H. Profiling of ribose methylations in RNA by high throughput sequencing. Angewandte Chemie. 2015;542:451 5 for detailed description | GEO Accession:GSM4591060 | OTHER | TRANSCRIPTOMIC | other | SINGLE | ION_TORRENT | Ion Torrent Proton | SRP265951 | intentional duplicate | GSE151797_Reference_sequence.fa PM_3.bam | bam bam | 215125332.0 | 6403488.0 | GSM4591060 r1 | 0:33.60 | A:50202267;C:65116674;G:52637331;T:47169060;N:0 | 33 | 50202267 | 65116674 | 52637331 | 47169060 | 0 | SRX8469993 | SRS6770644 | SRA1083099 | GEO | RNA Group - Prof. Henrik Nielsen, Department of Cellular and Molecular Medicine, University of Copenhagen | 1 | 0.73594 | 0.21617 | 0.86251 | 0.68892 | 42 | B | usable mapping rate | ion_torrent | ion_torrent | 5prime | small_rna | unknown | bulk | unknown | unknown | Denmark | 2020-06-04 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||
| 59504 | 59504 | SRR11924318 | SRX8469992 | SRS6770643 | SRP265951 | PRJNA637293 | The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2' O Me sites | GSE151797 | Other | A sequencing based profiling method RiboMeth seq for ribose methylations was used to study methylation patterns during Zebrafish Danio rerio development Overall design: All samples were analyzed in biological triplicates except for adult tail trunk that was in duplicate. | pubmed:32912962 | PM 2 | GSM4591059 | source name:protruding mouth stage|tissue:protruding mouth stage|rna fraction:size fractionated 20 40 nt whole cell RNA | PM 2 | Library strategy: RiboMeth seq Barcode separation using python script Adaptor trimming using Cutadapt v. 2.0 Mapping to rRNA reference sequence using Bowtie2 v. 2.3.4.1 Counting read ends and calculating RiboMeth seq scores using python scripts The output FASTA files from small RNA seq were merged and used as the basis of the SNORD search and rRNA interaction prediction. Initially SNORDs were identified by running the merged FASTA file through snoScan Schattner et al. 2005 against zebrafish early and late rRNA reference sequences Locati et al. 2017. Genome build: early and late zebrafish rRNA locati et al. The reference sequences are available in the FASTA file on the series record. Supplementary files format and content: MS Excel file contains five prime and three prime read count and calculated RiboMeth seq score at all positions in the rRNA sequence. | protruding mouth stage | Tissues were homogenized and whole cell RNA was extracted using Qiazol Qiagen according to the manufacturer. RiboMeth seq: 5 10 ug of RNA was partially degraded by alkaline at denaturing temperatures. The size fraction 20 40 nt was purified on gels and linkers added using a system relying on a modified Arabidopsis tRNA ligase joining 2' three prime cyclic phosphate and five prime phosphate ends. The library fragments were then sequenced on the Ion Proton platform. See Birkedal U Christensen Dalsgaard M Krogh N Sabarinathan R Gorodkin J Nielsen H. Profiling of ribose methylations in RNA by high throughput sequencing. Angewandte Chemie. 2015;542:451 5 for detailed description | tissue:protruding mouth stage|rna fraction:size fractionated 20 40 nt whole cell RNA | GSM4591059 | GSM4591059: PM 2; Danio rerio; OTHER | GSM4591059 | 1 | Tissues were homogenized and whole cell RNA was extracted using Qiazol Qiagen according to the manufacturer. RiboMeth seq: 5 10 ug of RNA was partially degraded by alkaline at denaturing temperatures. The size fraction 20 40 nt was purified on gels and linkers added using a system relying on a modified Arabidopsis tRNA ligase joining 2' three prime cyclic phosphate and five prime phosphate ends. The library fragments were then sequenced on the Ion Proton platform. See Birkedal U Christensen Dalsgaard M Krogh N Sabarinathan R Gorodkin J Nielsen H. Profiling of ribose methylations in RNA by high throughput sequencing. Angewandte Chemie. 2015;542:451 5 for detailed description | GEO Accession:GSM4591059 | OTHER | TRANSCRIPTOMIC | other | SINGLE | ION_TORRENT | Ion Torrent Proton | SRP265951 | intentional duplicate | GSE151797_Reference_sequence.fa PM_2.bam | bam bam | 426315355.0 | 12915702.0 | GSM4591059 r1 | 0:33.01 | A:92276988;C:141702085;G:104175159;T:88161123;N:0 | 33 | 92276988 | 141702085 | 104175159 | 88161123 | 0 | SRX8469992 | SRS6770643 | SRA1083099 | GEO | RNA Group - Prof. Henrik Nielsen, Department of Cellular and Molecular Medicine, University of Copenhagen | 1 | 0.76385 | 0.1921 | 0.87207 | 0.71495 | 40 | B | usable mapping rate | ion_torrent | ion_torrent | 5prime | small_rna | unknown | bulk | unknown | unknown | Denmark | 2020-06-04 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||
| 59505 | 59505 | SRR11924317 | SRX8469990 | SRS6770641 | SRP265951 | PRJNA637293 | The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2' O Me sites | GSE151797 | Other | A sequencing based profiling method RiboMeth seq for ribose methylations was used to study methylation patterns during Zebrafish Danio rerio development Overall design: All samples were analyzed in biological triplicates except for adult tail trunk that was in duplicate. | pubmed:32912962 | PM 1 | GSM4591058 | source name:protruding mouth stage|tissue:protruding mouth stage|rna fraction:size fractionated 20 40 nt whole cell RNA | PM 1 | Library strategy: RiboMeth seq Barcode separation using python script Adaptor trimming using Cutadapt v. 2.0 Mapping to rRNA reference sequence using Bowtie2 v. 2.3.4.1 Counting read ends and calculating RiboMeth seq scores using python scripts The output FASTA files from small RNA seq were merged and used as the basis of the SNORD search and rRNA interaction prediction. Initially SNORDs were identified by running the merged FASTA file through snoScan Schattner et al. 2005 against zebrafish early and late rRNA reference sequences Locati et al. 2017. Genome build: early and late zebrafish rRNA locati et al. The reference sequences are available in the FASTA file on the series record. Supplementary files format and content: MS Excel file contains five prime and three prime read count and calculated RiboMeth seq score at all positions in the rRNA sequence. | protruding mouth stage | Tissues were homogenized and whole cell RNA was extracted using Qiazol Qiagen according to the manufacturer. RiboMeth seq: 5 10 ug of RNA was partially degraded by alkaline at denaturing temperatures. The size fraction 20 40 nt was purified on gels and linkers added using a system relying on a modified Arabidopsis tRNA ligase joining 2' three prime cyclic phosphate and five prime phosphate ends. The library fragments were then sequenced on the Ion Proton platform. See Birkedal U Christensen Dalsgaard M Krogh N Sabarinathan R Gorodkin J Nielsen H. Profiling of ribose methylations in RNA by high throughput sequencing. Angewandte Chemie. 2015;542:451 5 for detailed description | tissue:protruding mouth stage|rna fraction:size fractionated 20 40 nt whole cell RNA | GSM4591058 | GSM4591058: PM 1; Danio rerio; OTHER | GSM4591058 | 1 | Tissues were homogenized and whole cell RNA was extracted using Qiazol Qiagen according to the manufacturer. RiboMeth seq: 5 10 ug of RNA was partially degraded by alkaline at denaturing temperatures. The size fraction 20 40 nt was purified on gels and linkers added using a system relying on a modified Arabidopsis tRNA ligase joining 2' three prime cyclic phosphate and five prime phosphate ends. The library fragments were then sequenced on the Ion Proton platform. See Birkedal U Christensen Dalsgaard M Krogh N Sabarinathan R Gorodkin J Nielsen H. Profiling of ribose methylations in RNA by high throughput sequencing. Angewandte Chemie. 2015;542:451 5 for detailed description | GEO Accession:GSM4591058 | OTHER | TRANSCRIPTOMIC | other | SINGLE | ION_TORRENT | Ion Torrent Proton | SRP265951 | intentional duplicate | GSE151797_Reference_sequence.fa PM_1.bam | bam bam | 130388595.0 | 5124384.0 | GSM4591058 r1 | 0:25.44 | A:27290253;C:42592303;G:31075292;T:29430747;N:0 | 25 | 27290253 | 42592303 | 31075292 | 29430747 | 0 | SRX8469990 | SRS6770641 | SRA1083099 | GEO | RNA Group - Prof. Henrik Nielsen, Department of Cellular and Molecular Medicine, University of Copenhagen | 1 | 0.78884 | 0.23004 | 0.85113 | 0.67571 | 38 | B | usable mapping rate | ion_torrent | ion_torrent | 5prime | small_rna | unknown | bulk | unknown | unknown | Denmark | 2020-06-04 | Larval | Larval | Undetermined | Undetermined |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;