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run_metadata

12 rows where experiment.library_selection = "cDNA_randomPriming" and tissue_curation_coarse = "Surface Structure"

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tissue_curation_coarse 1

  • Surface Structure · 12 ✖

tissue_curation 1

technology 1

experiment.platform 1

experiment.library_strategy 1

experiment.library_source 1

experiment.library_selection 1

  • cDNA_randomPriming · 12 ✖

experiment.library_layout 1

devstage_curation_coarse 1

devstage_curation 1

Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
35980 35980 SRR33796117 SRX29017800 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P2000 1 P2000 1 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P2000_1_1.fq.gz P2000_1_2.fq.gz fastq fastq 6682157100.0 22273857.0 P2000 1 1.fq.gz 0:150 1:150 A:1750970707;C:1591819991;G:1593263349;T:1746103053;N:0 150 150     1750970707 1591819991 1593263349 1746103053 0 SRX29017800 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35981 35981 SRR33796118 SRX29017799 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P1000 3 P1000 3 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P1000_3_1.fq.gz P1000_3_2.fq.gz fastq fastq 6596943000.0 21989810.0 P1000 3 1.fq.gz 0:150 1:150 A:1725528987;C:1574140428;G:1576046656;T:1721226929;N:0 150 150     1725528987 1574140428 1576046656 1721226929 0 SRX29017799 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35982 35982 SRR33796119 SRX29017798 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P1000 2 P1000 2 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P1000_2_1.fq.gz P1000_2_2.fq.gz fastq fastq 6677331000.0 22257770.0 P1000 2 1.fq.gz 0:150 1:150 A:1758237128;C:1582217484;G:1584403835;T:1752472553;N:0 150 150     1758237128 1582217484 1584403835 1752472553 0 SRX29017798 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35983 35983 SRR33796120 SRX29017797 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P1000 1 P1000 1 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P1000_1_1.fq.gz P1000_1_2.fq.gz fastq fastq 6771926100.0 22573087.0 P1000 1 1.fq.gz 0:150 1:150 A:1788071352;C:1600799117;G:1602227700;T:1780827931;N:0 150 150     1788071352 1600799117 1602227700 1780827931 0 SRX29017797 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35984 35984 SRR33796121 SRX29017796 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P500 3 P500 3 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P500_3_1.fq.gz P500_3_2.fq.gz fastq fastq 6691956300.0 22306521.0 P500 3 1.fq.gz 0:150 1:150 A:1763763611;C:1584417511;G:1585842124;T:1757933054;N:0 150 150     1763763611 1584417511 1585842124 1757933054 0 SRX29017796 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35985 35985 SRR33796122 SRX29017795 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P500 2 P500 2 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P500_2_1.fq.gz P500_2_2.fq.gz fastq fastq 6720858300.0 22402861.0 P500 2 1.fq.gz 0:150 1:150 A:1752279513;C:1609781801;G:1611483135;T:1747313851;N:0 150 150     1752279513 1609781801 1611483135 1747313851 0 SRX29017795 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35986 35986 SRR33796123 SRX29017794 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P500 1 P500 1 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P500_1_1.fq.gz P500_1_2.fq.gz fastq fastq 6820862100.0 22736207.0 P500 1 1.fq.gz 0:150 1:150 A:1812260874;C:1601860387;G:1602603805;T:1804137034;N:0 150 150     1812260874 1601860387 1602603805 1804137034 0 SRX29017794 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35987 35987 SRR33796124 SRX29017793 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body MX 3 MX 3 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     MX_3_1.fq.gz MX_3_2.fq.gz fastq fastq 6795327600.0 22651092.0 MX 3 1.fq.gz 0:150 1:150 A:1768016462;C:1630727253;G:1632326554;T:1764257331;N:0 150 150     1768016462 1630727253 1632326554 1764257331 0 SRX29017793 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35988 35988 SRR33796125 SRX29017792 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P2000 3 P2000 3 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P2000_3_1.fq.gz P2000_3_2.fq.gz fastq fastq 6620786400.0 22069288.0 P2000 3 1.fq.gz 0:150 1:150 A:1736628496;C:1574653543;G:1577791512;T:1731712849;N:0 150 150     1736628496 1574653543 1577791512 1731712849 0 SRX29017792 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35989 35989 SRR33796126 SRX29017791 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body P2000 2 P2000 2 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     P2000_2_1.fq.gz P2000_2_2.fq.gz fastq fastq 6613914000.0 22046380.0 P2000 2 1.fq.gz 0:150 1:150 A:1733574880;C:1575483348;G:1576793005;T:1728062767;N:0 150 150     1733574880 1575483348 1576793005 1728062767 0 SRX29017791 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35990 35990 SRR33796127 SRX29017790 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body MX 2 MX 2 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     MX_2_1.fq.gz MX_2_2.fq.gz fastq fastq 6769929300.0 22566431.0 MX 2 1.fq.gz 0:150 1:150 A:1773923783;C:1611785261;G:1615111695;T:1769108561;N:0 150 150     1773923783 1611785261 1615111695 1769108561 0 SRX29017790 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure
35991 35991 SRR33796128 SRX29017789 SRS25233880 SRP589371 PRJNA1256770 Danio rerio Raw sequence reads PRJNA1256770 Whole Genome Sequencing Transcriptome sequencing was used to identify genes regulated by the polysaccharides that affect the immune activity of zebrafish.       Model organism or animal sample from Danio rerio Zebrafish treatment with polysaccharides   strain:transgenic neutrophil green fluorescent MPX|age:3 dpf|dev stage:embryo|collection date:2024 05 01|geo loc name:China: Zhejiang|sex:pooled male and female|tissue:whole body|disease:Immunosuppression|MX:control sample|P500:immune suppression model treated with 500 ug/mL polysaccharide|P1000:immune suppression model treated with 1000 ug/mL polysaccharide|P2000:immune suppression model treated with 2000 ug/mL polysaccharide|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: embryo whole body MX 1 MX 1 Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using polyA selection and fragmented. First and second strand cDNA synthesis was performed followed by end repair A tailing and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ 2000RS platform with paired end 150 bp reads.     RNA-Seq TRANSCRIPTOMIC cDNA_randomPriming PAIRED BGISEQ MGISEQ-2000RS   SRP589371     MX_1_1.fq.gz MX_1_2.fq.gz fastq fastq 6715295400.0 22384318.0 MX 1 1.fq.gz 0:150 1:150 A:1774590051;C:1584963102;G:1587348964;T:1768393283;N:0 150 150     1774590051 1584963102 1587348964 1768393283 0 SRX29017789 SRS25233880 SRA2141272 West Anhui Universiaty|College of Biotechnology and Pharmaceutical Engine West Anhui Universiaty                       B B biological fallback assumption bgi bgi unknown random_priming unknown bulk unknown unknown   China 2025-06-02 Larval Larval Trunk Surface Structure

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;